CN106376544B - Protective solution for preserving artemia nauplii and preserving method - Google Patents

Protective solution for preserving artemia nauplii and preserving method Download PDF

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Publication number
CN106376544B
CN106376544B CN201610783273.7A CN201610783273A CN106376544B CN 106376544 B CN106376544 B CN 106376544B CN 201610783273 A CN201610783273 A CN 201610783273A CN 106376544 B CN106376544 B CN 106376544B
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preservation
artemia nauplii
angelica
protective solution
solution
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CN106376544A (en
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于宏
孙朦朦
路斌
王永苓
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Tianjin Ocean Pal Carol Biotech Co Ltd
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Tianjin Ocean Pal Carol Biotech Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a protective solution for preserving artemia nauplii and a preservation method, wherein the protective solution comprises 5-15 mg/L of angelica polysaccharide with the following concentration. The density of the artemia nauplii in the protective solution is 1-30 per mL. The preservation temperature is 0-10 ℃; the pH value of the preservation solution is 7-10. The invention can ensure the stability of the survival rate of the artemia nauplii. By controlling factors such as temperature, the survival rate of the artemia nauplii can be effectively ensured at a low temperature, and the survival rate of the artemia nauplii reaches over 80% in 24 hours, so that the problem of low survival rate of the artemia nauplii at the low temperature is solved.

Description

Protective solution for preserving artemia nauplii and preserving method
Technical Field
The invention relates to the technical field of artemia breeding, in particular to a protective solution for preserving artemia nauplii and a preserving method.
Background
The artemia nauplii are generally used as living feeds for aquaculture, particularly living feeds for early larval stages of sea fishes and sea shrimps, and the living feeds such as the artemia nauplii are not generally available on the market, only artemia eggs are provided, the nauplii are hatched from the artemia eggs, and the hatching of the artemia eggs can be completed in hatching liquid, but at present, the research on the low-temperature storage method of the artemia nauplii is particularly few. If the problem is solved, the artemia nauplii can be directly sold, the burden of aquaculture enterprises is reduced, and the artemia hatching is more commercialized.
Disclosure of Invention
In view of the above, the present invention aims to provide a protection solution for preserving artemia nauplii and a preservation method thereof, so as to solve the problem of low survival rate of the artemia nauplii after low temperature preservation.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
a protective solution for preserving artemia nauplii comprises 5-15 mg/L of angelica polysaccharide.
Preferably, the Chinese angelica polysaccharide comprises 8-12 mg/L of Chinese angelica polysaccharide with the following concentration; preferably, 10 mg/L.
Preferably, the detergent also comprises components with the following concentration, wherein NaCl accounts for 15-25 g/L; MgSO (MgSO)40.002～0.008g/L；FeCl30.002～0.008g/L。
Preferably, the detergent also comprises the following components with the concentration of 18-22 g/L NaCl; MgSO (MgSO)40.004～0.006g/L；FeCl30.004～0.006g/L。
Preferably, the composition also comprises the following components with the concentration of 20g/L of NaCl; MgSO (MgSO)40.005g/L；FeCl30.005g/L。
Preferably, the angelica polysaccharide is obtained by extracting the following steps of processing fresh Chinese herbal medicine angelica into small blocks, naturally drying the small blocks, soaking the small blocks in ethanol for more than one week until the color of the ethanol is unchanged, boiling the processed angelica in water for 20-40 min, keeping the liquid, and precipitating the liquid with ethanol to obtain floccule, namely the angelica polysaccharide; preferably, boiling for 30 min.
Preferably, the angelica is soaked in the ethanol with the proportion of 0.8-1.2 g of the angelica and 30-50 mL of ethanol; the angelica was soaked in 1g of angelica in 40mL of ethanol.
The invention also provides a method for preserving artemia by using the protective solution for preserving the artemia nauplii, which is characterized by comprising the following steps: the density of the artemia nauplii in the protective solution is 1-30 per mL.
Preferably, the preservation temperature is 0-10 ℃; the pH value of the preservation solution is 7-10.
Preferably, the preservation temperature is 2-6 ℃; the pH value of the preservation solution is 8-9.
Compared with the prior art, the protection solution for preserving the artemia nauplii and the preservation method have the following advantages:
(1) the protection solution and the method provided by the invention realize the storage problem of the artemia nauplii in a low-temperature state and solve the problem of short storage time of the artemia nauplii in the low-temperature state.
(2) The protection solution and the method can ensure the stability of the survival rate of the artemia nauplii. By controlling factors such as temperature, the survival rate of the artemia nauplii can be effectively ensured at a low temperature, and the survival rate of the artemia nauplii reaches over 80% in 24 hours, so that the problem of low survival rate of the artemia nauplii at the low temperature is solved.
(3) The present invention is also suitable for ensuring the survival rate of stable artemia nauplii in large hatcheries.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The present invention will be described in detail with reference to examples.
Example one
Hatching is carried out by using a transparent incubator and a 250ml hatching tube, and hatching is carried out by using 200ml hatching solution in the hatching tube. The hatching fluid is prepared by preparing artificial seawater crystal from tap water, wherein the salinity is 22g dissolved salt/L, the pH is 8.5, the concentration of dissolved oxygen is more than 6mg/L, and the water temperature is 25 ℃;
the incubated artemia nauplii are added into a protective solution, and the temperature of the protective solution is 25 ℃.
The density of the artemia nauplii in the protective solution is 30 per mL; and uses the decapsulated Bohai Bay insect egg.
After being stored for 24 hours at the temperature of 4 ℃, the survival rate of the artemia nauplii reaches 80 percent.
The protective solution comprises the following components with the concentration of 10mg/L angelica polysaccharide; NaCl 20 g/L; MgSO (MgSO)40.005g/L；FeCl30.005g/L。
The angelica polysaccharide is obtained by extracting the following steps of processing fresh Chinese herbal medicine angelica into small blocks, naturally drying the small blocks, soaking the small blocks in ethanol for more than one week until the color of the ethanol is unchanged, boiling the processed angelica in water for 30min, reserving liquid, and precipitating the liquid with ethanol to obtain floccule, namely the angelica polysaccharide. The angelica was soaked in 1g of angelica in 40mL of ethanol.
Example two
Hatching was carried out using a transparent incubator and a 250ml hatching tube, and hatching was carried out using 200ml hatching fluid in the hatching tube. Hatching is carried out by using a transparent incubator and a 250ml hatching tube, and hatching is carried out by using 200ml hatching solution in the hatching tube. The hatching fluid is prepared by preparing artificial seawater crystal from tap water, wherein the salinity is 22g dissolved salt/L, the pH is 8.5, the concentration of dissolved oxygen is more than 6mg/L, and the water temperature is 25 ℃;
the incubated artemia nauplii are added to a protective solution at a temperature of 25 ℃.
The density of the artemia nauplii in the protective solution is 30 per mL; and uses Bohai Bay insect eggs.
After being stored for 24 hours at the temperature of 4 ℃, the survival rate of the artemia nauplii reaches 80 percent.
The protective solution comprises the following components with the concentration of 9mg/L angelica polysaccharide; 19g/L of NaCl; MgSO (MgSO)40.004g/L；FeCl30.004g/L。
The angelica polysaccharide is obtained by extracting the following steps of processing fresh Chinese herbal medicine angelica into small blocks, naturally drying the small blocks, soaking the small blocks in ethanol for more than one week until the color of the ethanol is unchanged, boiling the processed angelica in water for 30min, reserving liquid, and precipitating the liquid with ethanol to obtain floccule, namely the angelica polysaccharide. The angelica was soaked in 1g of angelica in 40mL of ethanol.
EXAMPLE III
Hatching is carried out by using a transparent incubator and a 250ml hatching tube, and hatching is carried out by using 200ml hatching solution in the hatching tube. The hatching fluid is prepared by preparing artificial seawater crystal from tap water, wherein the salinity is 22g dissolved salt/L, the pH is 8.5, the concentration of dissolved oxygen is more than 6mg/L, and the water temperature is 25 ℃;
the incubated artemia nauplii are added to a protective solution at a temperature of 4 ℃.
The density of the artemia nauplii in the protective solution is 30 per mL; and uncoating Bohai Bay insect eggs are used.
After being stored for 24 hours at the temperature of 4 ℃, the survival rate of the artemia nauplii reaches 80 percent.
The protective solution comprises the following components with the concentration of 11mg/L angelica polysaccharide; NaCl 21 g/L; MgSO (MgSO)40.006g/L；FeCl30.006g/L。
The angelica polysaccharide is obtained by extracting the following steps of processing fresh Chinese herbal medicine angelica into small blocks, naturally drying the small blocks, soaking the small blocks in ethanol for more than one week until the color of the ethanol is unchanged, boiling the processed angelica in water for 30min, reserving liquid, and precipitating the liquid with ethanol to obtain floccule, namely the angelica polysaccharide. The angelica was soaked in 1g of angelica in 40mL of ethanol.
The decapsulated treated bohai gulf artemia cysts used in example two.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the invention, so that any modifications, equivalents, improvements and the like, which are within the spirit and principle of the present invention, should be included in the scope of the present invention.

Claims (12)

1. A protection solution for preserving artemia nauplii is characterized in that: comprises 5-15 mg/L of angelica polysaccharide with the following concentration; 15-25 g/L of NaCl; MgSO (MgSO)40.002～0.008g/L；FeCl30.002-0.008 g/L; the preservation temperature is 0-10 ℃; the pH value of the preservation solution is 7-10.
2. The protective solution for artemia nauplii preservation according to claim 1, wherein: comprises 8-12 mg/L of angelica polysaccharide with the following concentration.
3. The protective solution for artemia nauplii preservation according to claim 1, wherein: comprises the following concentration of 10mg/L angelica polysaccharide.
4. A protective solution for the preservation of artemia nauplii according to any one of claims 1 to 3, wherein the protective solution is for the preservation of artemia naupliiIn the following steps: NaCl 18-22 g/L; MgSO (MgSO)40.004～0.006g/L；FeCl30.004～0.006g/L。
5. The protective solution for artemia nauplii preservation according to any one of claims 1 to 3, wherein: NaCl 20 g/L; MgSO (MgSO)40.005g/L；FeCl30.005g/L。
6. The protective solution for artemia nauplii preservation according to any one of claims 1 to 3, wherein: the angelica polysaccharide is obtained by extracting the following steps of processing fresh Chinese herbal medicine angelica into small blocks, naturally drying the small blocks, soaking the small blocks in ethanol for more than one week until the color of the ethanol is unchanged, boiling the processed angelica in water for 20-40 min, retaining liquid, and precipitating the liquid with ethanol to obtain floccule, namely the angelica polysaccharide.
7. The protective solution for artemia nauplii preservation according to claim 6, wherein: the treated radix Angelicae sinensis is boiled in water for 30 min.
8. The protective solution for artemia nauplii preservation according to claim 6, wherein: soaking the angelica in 0.8-1.2 g of angelica and 30-50 mL of ethanol.
9. The protective solution for artemia nauplii preservation according to claim 8, wherein: the angelica was soaked in 1g of angelica in 40mL of ethanol.
10. A method of artemia preservation using the protective solution for artemia nauplii preservation according to any one of claims 1 to 9, wherein: the density of the artemia nauplii in the protective solution is 1-30 per mL.
11. The method of claim 10, wherein: the preservation temperature is 0-10 ℃; the pH value of the preservation solution is 7-10.
12. The method of claim 10, wherein: the preservation temperature is 2-6 ℃; the pH value of the preservation solution is 8-9.
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CN107347872B (en) * 2017-06-30 2021-11-19 天津海友佳音生物科技股份有限公司 Formula and method for freezing, storing and hatching artemia unshelled eggs

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CN101235097A (en) * 2008-02-29 2008-08-06 武汉大学 Method for extracting polysaccharide from angelica and application thereof
CN102630663A (en) * 2012-01-10 2012-08-15 李新书 Method for storing shelled brine shrimp eggs
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CN102630663A (en) * 2012-01-10 2012-08-15 李新书 Method for storing shelled brine shrimp eggs
CN103374526A (en) * 2012-04-18 2013-10-30 上海市农业科学院 Coccidian oocyst culture preservative fluid and application method thereof
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