KR101800810B1 - Method for prevention and treatment for scuticociliatosis in fishes using blue light emitting diode(LED) - Google Patents
Method for prevention and treatment for scuticociliatosis in fishes using blue light emitting diode(LED) Download PDFInfo
- Publication number
- KR101800810B1 KR101800810B1 KR1020160022617A KR20160022617A KR101800810B1 KR 101800810 B1 KR101800810 B1 KR 101800810B1 KR 1020160022617 A KR1020160022617 A KR 1020160022617A KR 20160022617 A KR20160022617 A KR 20160022617A KR 101800810 B1 KR101800810 B1 KR 101800810B1
- Authority
- KR
- South Korea
- Prior art keywords
- fish
- light source
- wavelength
- led
- led light
- Prior art date
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 66
- 235000019688 fish Nutrition 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims abstract description 35
- 230000002265 prevention Effects 0.000 title claims description 5
- 230000001678 irradiating effect Effects 0.000 claims abstract description 15
- 210000004027 cell Anatomy 0.000 claims description 51
- 241000269908 Platichthys flesus Species 0.000 claims description 28
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- 206010021033 Hypomenorrhoea Diseases 0.000 claims description 5
- 239000003504 photosensitizing agent Substances 0.000 claims description 5
- 241000252233 Cyprinus carpio Species 0.000 claims description 4
- 241001125048 Sardina Species 0.000 claims description 4
- 241000269821 Scombridae Species 0.000 claims description 4
- 235000020640 mackerel Nutrition 0.000 claims description 4
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 claims description 4
- 235000019512 sardine Nutrition 0.000 claims description 4
- 241000972773 Aulopiformes Species 0.000 claims description 3
- 241001454694 Clupeiformes Species 0.000 claims description 3
- 235000019513 anchovy Nutrition 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 235000019515 salmon Nutrition 0.000 claims description 3
- MHIITNFQDPFSES-UHFFFAOYSA-N 25,26,27,28-tetrazahexacyclo[16.6.1.13,6.18,11.113,16.019,24]octacosa-1(25),2,4,6,8(27),9,11,13,15,17,19,21,23-tridecaene Chemical class N1C(C=C2C3=CC=CC=C3C(C=C3NC(=C4)C=C3)=N2)=CC=C1C=C1C=CC4=N1 MHIITNFQDPFSES-UHFFFAOYSA-N 0.000 claims description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 2
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 claims description 2
- ZGXJTSGNIOSYLO-UHFFFAOYSA-N 88755TAZ87 Chemical compound NCC(=O)CCC(O)=O ZGXJTSGNIOSYLO-UHFFFAOYSA-N 0.000 claims description 2
- 241000252203 Clupea harengus Species 0.000 claims description 2
- 241001149724 Cololabis adocetus Species 0.000 claims description 2
- 241000276457 Gadidae Species 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 claims description 2
- 241001282110 Pagrus major Species 0.000 claims description 2
- 241000276498 Pollachius virens Species 0.000 claims description 2
- 241001529596 Pontinus kuhlii Species 0.000 claims description 2
- 241000277331 Salmonidae Species 0.000 claims description 2
- 241000269851 Sarda sarda Species 0.000 claims description 2
- 241001441724 Tetraodontidae Species 0.000 claims description 2
- 229960002749 aminolevulinic acid Drugs 0.000 claims description 2
- 210000004081 cilia Anatomy 0.000 claims description 2
- 235000019514 herring Nutrition 0.000 claims description 2
- MOFVSTNWEDAEEK-UHFFFAOYSA-M indocyanine green Chemical compound [Na+].[O-]S(=O)(=O)CCCCN1C2=CC=C3C=CC=CC3=C2C(C)(C)C1=CC=CC=CC=CC1=[N+](CCCCS([O-])(=O)=O)C2=CC=C(C=CC=C3)C3=C2C1(C)C MOFVSTNWEDAEEK-UHFFFAOYSA-M 0.000 claims description 2
- 229960004657 indocyanine green Drugs 0.000 claims description 2
- DZVCFNFOPIZQKX-LTHRDKTGSA-M merocyanine Chemical compound [Na+].O=C1N(CCCC)C(=O)N(CCCC)C(=O)C1=C\C=C\C=C/1N(CCCS([O-])(=O)=O)C2=CC=CC=C2O\1 DZVCFNFOPIZQKX-LTHRDKTGSA-M 0.000 claims description 2
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 2
- IEQIEDJGQAUEQZ-UHFFFAOYSA-N phthalocyanine Chemical compound N1C(N=C2C3=CC=CC=C3C(N=C3C4=CC=CC=C4C(=N4)N3)=N2)=C(C=CC=C2)C2=C1N=C1C2=CC=CC=C2C4=N1 IEQIEDJGQAUEQZ-UHFFFAOYSA-N 0.000 claims description 2
- 150000004032 porphyrins Chemical class 0.000 claims description 2
- 229950003937 tolonium Drugs 0.000 claims description 2
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 claims description 2
- MYDZUHZYHKZGBW-UHFFFAOYSA-N formamide;sodium Chemical compound [Na].NC=O MYDZUHZYHKZGBW-UHFFFAOYSA-N 0.000 claims 1
- 230000002165 photosensitisation Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 18
- 238000005259 measurement Methods 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 102100037600 P2Y purinoceptor 1 Human genes 0.000 description 9
- 108050008996 P2Y purinoceptor 1 Proteins 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 230000006378 damage Effects 0.000 description 6
- 230000008602 contraction Effects 0.000 description 5
- 230000001186 cumulative effect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000497908 Miamiensis avidus Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 230000004899 motility Effects 0.000 description 4
- 244000045947 parasite Species 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000238366 Cephalopoda Species 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 239000003610 charcoal Substances 0.000 description 3
- 230000009849 deactivation Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012091 fetal bovine serum Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 201000009030 Carcinoma Diseases 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 240000005546 Piper methysticum Species 0.000 description 2
- 235000016787 Piper methysticum Nutrition 0.000 description 2
- 241000382353 Pupa Species 0.000 description 2
- 230000002141 anti-parasite Effects 0.000 description 2
- 230000001640 apoptogenic effect Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 210000005013 brain tissue Anatomy 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 208000010932 epithelial neoplasm Diseases 0.000 description 2
- 231100000636 lethal dose Toxicity 0.000 description 2
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 108010009004 proteose-peptone Proteins 0.000 description 2
- 239000003642 reactive oxygen metabolite Substances 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000219357 Cactaceae Species 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001125831 Istiophoridae Species 0.000 description 1
- 241001280377 Oncorhynchus tshawytscha Species 0.000 description 1
- 241000269982 Paralichthys Species 0.000 description 1
- 241000269978 Pleuronectiformes Species 0.000 description 1
- 241000096946 Pseudocohnilembus persalinus Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000209051 Saccharum Species 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 241001491881 Uronema marinum Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 231100000676 disease causative agent Toxicity 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000009372 pisciculture Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
- A61L2/0029—Radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
- A01K63/006—Accessories for aquaria or terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21K—NON-ELECTRIC LIGHT SOURCES USING LUMINESCENCE; LIGHT SOURCES USING ELECTROCHEMILUMINESCENCE; LIGHT SOURCES USING CHARGES OF COMBUSTIBLE MATERIAL; LIGHT SOURCES USING SEMICONDUCTOR DEVICES AS LIGHT-GENERATING ELEMENTS; LIGHT SOURCES NOT OTHERWISE PROVIDED FOR
- F21K9/00—Light sources using semiconductor devices as light-generating elements, e.g. using light-emitting diodes [LED] or lasers
- F21K9/60—Optical arrangements integrated in the light source, e.g. for improving the colour rendering index or the light extraction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Optics & Photonics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Zoology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a method of preventing or treating scuticacidosis of fish using blue LEDs, and more particularly, to a method of preventing or treating scuticacidosis of fishes having excellent sterilization or bacteriostatic effect against scuticacid when irradiated with blue LED Or a method of treatment. The method for preventing or treating scutchikosis of fish according to the present invention is carried out by irradiating an LED light source of blue light to an underwater environment including fishes, thereby being environmentally friendly and economical. In addition to increasing productivity of fishes, Can be saved.
Description
The present invention relates to a method of preventing or treating scuticacidosis of fish using blue LEDs, and more particularly, to a method of preventing or treating scuticacidosis of fishes having excellent sterilization or bacteriostatic effect against scuticacid when irradiated with blue LED Or a method of treatment.
Among the aquaculture flounder, the flounder is fast growing and highly active in the production, which accounts for a large part of the total fish production. Domestic flounder farms are distributed in Jeju Island, Wando in the southern coast, part of Geoje Island, Busan, and Gyeongju Gampo area on the East Coast, and most of the flounder farms are concentrated in Jeju Island.
Sututika spp. Is a 30 ~ 40 ㎛ ciliate, and it is often found in artificial seedling and amphibian of flounder. It has been reported that Sukutika is largely induced in fish body surface, fins, kidney, blood vessels and brain tissues, and mass destruction of flounder mostly through destruction of plastic connective tissue. In addition, unlike other parasites of fish, it penetrates into brain tissue through blood vessels and is parasitic. There is a great deal of damage from the flounder to the adult fish. In flounder fish farms, formalin for fish is used as an insecticide for Sukutika, and it is considered that this drug can cause some damage to the host fish, and its effectiveness is not high. It is also a less desirable method in terms of food safety and ecosystem conservation.
Therefore, it is urgent to develop a method for relieving the scuticacid of fish which is safe for fish and is not harmful to the aquaculture environment while being effective in relieving sututika.
The inventors of the present invention have found that when an LED light source of blue light is irradiated to an underwater environment including fishes while searching for a method of preventing or treating scuticacidosis of fish, The inventors confirmed that they can be prevented or treated in an environmentally friendly manner without using antibiotics and completed the present invention.
Accordingly, the present invention provides a method for preventing or treating scutchikosis of fish, comprising irradiating an LED light source of blue light to an underwater environment including fish.
The present invention also provides a device for preventing or treating scutchikosis of fish, comprising a system for irradiating an LED light source of blue light to an underwater environment including fish.
In order to achieve the above object,
The present invention
A method for preventing or treating scutchikosis of fish, comprising the step of irradiating an LED light source of blue light to an underwater environment including fish.
In addition,
A system for preventing or treating scutchikosis in fish, comprising a system for irradiating an LED light source of blue light to an underwater environment including fishes.
Hereinafter, the present invention will be described in detail.
The present invention provides a method for preventing or treating scutchikosis of fish, comprising irradiating an LED light source of blue light to an underwater environment including fish.
In the fish farming industry, various breeding systems are being developed to increase productivity and to prevent or treat diseases. However, there is a demand for measures to maximize the productivity with minimum investment cost in breeding farms.
The method for preventing or treating scutchikosis of fish according to the present invention is a method for preventing or treating scuticacid in a fish by irradiating an LED light source of a specific wavelength to an underwater environment including a fish to sterilize or bacterium the fullness of the scutica without causing any physiological or physical damage to the fish .
The LED (LED) is a light source for sterilization, exposure and identification as well as general illumination. It is used for lighting plants, insect pest control, light source for attracting fish in the sea, light source for medical and skin care, It is mainly used for special purposes. These LEDs illuminate various colors of light (Red, Blue, Green, White, Yellow). Through the above-mentioned color light, it is possible to strengthen the tolerance to the stress of the fish and to promote the growth of the fish, Can
The LED light source may have one or more wavelengths, preferably a wavelength in the range of 380 to 480 nm, and is preferably maintained at a light amount in the range of 20 to 1500 占 ㆍ m 占 퐉 -2 s -1 . Concretely, the quantity of light in the range of 20 to 1000 占 mol m 占 ㆍ -2占-1 -1 for the wavelength of 405nm and the quantity of light in the range of 80 to 1500 占 ㆍ m 占 ㆍ 2占 퐏 -1 for the wavelength of 465nm are maintained .
The wavelength of the blue light LED light source and the range of the light amount are the same as those of the LED light source that does not cause physical damage such as inflammation or necrosis to the fish, And the amount of light.
The light period of the LED light source is preferably 6 to 12: 18 to 12 hours (L: D). According to one embodiment of the present invention, in case of Sucutika infected flounder, when the blue light of 6:18 hours (L: D) was irradiated for 24 days, the survival rate was as high as 80 to 90%.
The fish includes all kinds that can be infected by Sukhitaka, and can be cultured fish or aquarium fish. Specific examples include flounder, blowfish, carp, cilia, eel, trout, mackerel, mackerel, marlin, flounder, ten gangs, strength bridge, rockfish, uroguardia, sardine, pollock, codfish, saury, herring, eel, sardine, salmon, tuna , Bonito, anchovy, hawk, red sea bream, early stage, and the like.
The above-mentioned Sucutika spp. Refers to Miamiensis avidus, Uronema marinum or Pseudocohnilembus persalinus , which is the cause of infectious diseases of scutica, mainly M. avids , and can be separated from diseased flounder, etc. Also, proteose peptone, yeast (Epithelioma papulosum cyprini cell line or Chinook Salmon Enbryi cell line) containing yeast extract, sodium chloride, or Fetal Bovine Serum It is preferable to be cultured. This is because the culture medium composition can increase the expression of the strain, so that a large amount of pathogenic agent can be obtained.
The fish may include, but is not limited to, 1 to 1 x 10 < 8 > cells / ml concentration of scutica.
The fish may be infected with early, middle or late scuticacid, and preferably infected with early scuticacid.
When an LED light source of blue light is irradiated to the fish, active oxygen is generated in the fish, and the sututica can be destroyed.
The method for preventing or treating scuticacid according to the present invention can be carried out by treating fish with a photosensitizer and irradiating the LED light source with blue light. The photosensitizer can be activated by light to change its oxygen molecule (O 2 ) to active oxygen, to create new radicals, or to create new species and destroy pathogens. The photosensitizer material may be indocyanine green, methylene blue, toluidine blue, aminolevulinic acid, phthalocyanine, porphyrin, texapyrine, bacterioclomer, merocyanine, psoralen, benzoporphyrin derivatives, But is not limited thereto.
The underwater environment may be, but not limited to, a fish tank, an aquarium, a farm, a pond, a kennel, or the ocean.
The present invention also provides an apparatus for preventing or treating scutchikosis in fish, comprising a system for irradiating an LED light source of blue light to an underwater environment including fish.
For the prevention or treatment of scuticacid, conventional formalin or antibiotics have been added to the fish to be orally administered to the fish. However, such a method has been known to cause pathogenic bacteria to colonize and proliferate in the intestinal tract when a bacterial disease occurs, And the use of antibiotics is likely to produce resistant pathogens. On the other hand. When the method for preventing or treating scutchikosis using the blue light LED of the present invention is used, there is an advantage that the scuticosis of fish can be prevented or treated without any side effect.
The method for preventing or treating scutchikosis of fish according to the present invention is carried out by irradiating an LED light source of blue light to an underwater environment including fishes, thereby being environmentally friendly and economical. In addition to increasing productivity of fishes, Can be saved.
1 is a diagram showing a wavelength region (405, 465, 520, 640 nm) of an LED light source.
Fig. 2 is a graph showing the killing rate of Sutica kava according to irradiation time of LED wavelengths (405, 465, 520 and 640 nm).
Fig. 3 is a diagram showing the inactivation coefficients of Sutica cactus with time, treated with
FIG. 4 is a graph showing a change in the size of the Sutuca cordata according to the LED wavelength analysis using a flow cytometer. FIG.
FIG. 5 is a graph showing the rate of death of Sutica pupa according to an LED wavelength survey using a flow cytometer.
Fig. 6 is a graph showing the degree of active oxygen generated in a sutucca filament according to an LED wavelength survey. Fig.
7 is a graph showing the degree of deactivation of the Sutica cord according to the amount of LED light.
8 is a chart showing the degree of inactivation of Sutica spp according to the concentration of scutica.
Fig. 9 is a diagram showing the cumulative mortality rate of the flounder infected with scutichariae.
10 is a diagram showing an image of a flounder infected with scutichariae.
11 is a diagram showing the cumulative mortality rate of flounder infected with scutchikia after irradiating 405 nm LED light with 6L: 18D, 12L: 12D, 18L: 6D, 24L: 0D light period.
Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.
EXAMPLES Example 1. Preparation of LED light source and scutica
1-1. Preparation of LED light source
A light emitting diode (LED) light source was a blue light LED light source of 405, 465, 520, and 640 nm wavelengths supplied by Pukyong National University LED-Marine Fusion Technology Research Center and was measured with a radio meter (Biospherical Instruments Inc., USA) And the light quantity of each wavelength was measured. As a result, the amount of light was 250, 516, 303 and 586 μmol / m² ㆍ s, respectively.
The wavelength band of the LED light source is shown in FIG. 1 using a temperature-controlled integration sphere system (Withlight Co. Ltd., Korea).
1-2. Preparation of Sukutika
The causative agent of scuticus infection was obtained from diseased flounder, and then 2% proteose peptone, 1% yeast extract, 1.17% sodium chloride and 10% fetal bovine serum Bovine Serum) (P2Y1 medium) and incubated at 20 ° C for 3 days before use.
Example 2. Anti-parasitic effect according to LED wavelength
2-1. Effect of LED wavelength on Hansukutika
2-1-1. How to measure
First, the concentration of parasites was measured using a hemocytometer in a scuticacid cultured according to Example 1, suspended in P2Y1 medium to have a parasite concentration of 10 5 cells / ml, and 5 ml Was inoculated into a culture flask (T25). Subsequently, 405, 465, 520, and 640 nm wavelengths of the dark region (control group) and the maximum light amount were irradiated and reacted at 20 ° C. After 3, 6, 12, 24, and 48 hours, The cells were stained with the same amount of 0.25% tryphan blue for 5 minutes, and the total cell count and the number of stained cells were counted using a hemocytometer.
2-1-2. Measurement result
The killing rate of Sutica kava (10 5 cells / ml) according to irradiation time of
Expression (a): The death of the killed Sututika ( M. avidus ) Ratio (%) = number of stained cells on the trypan blue / total number of cells
As shown in FIG. 2, it was interesting to note that all the Sutica kernels were killed at a wavelength of 405 nm for 24 hours, and 80% of Sutica kernels were killed at a wavelength of 465 nm for 48 hours. In the dark zone (control) Most of the Sukutika populations survived the 640nm wavelength.
From the above results, it can be seen that the blue wavelength band of 405 nm and 465 nm is a wavelength band capable of directly killing M. avidus .
2-2. Quantitative analysis of the effect of Hanskutika
2-2-1. How to measure
In order to compare the effectiveness of Hanskutika at the wavelengths of 405nm and 465nm, which are effective wavelengths of scuticacid killing, compared to formalin, which is the only existing treatment method, formalin concentration higher than 100-200ppm, which is the recommended concentration of domestic scutica, is 200 and 400ppm And the experiment was conducted. Briefly, in a T25 flask, 5 ml of 5 × 10 4 cells / ml of scutica charcoal suspended in P2Y1 medium was inoculated, and then the dark section, the
2-2-2. Measurement result
The inactivation coefficients of Sutuca cordata treated with
As shown in FIG. 3, in the irradiated section of 405 nm wavelength, significant decrease of Sukutika was observed compared to the dark period (control group) from 3 hours. After 6 hours, all the Sukutika larvae lost their motility, but the formalin treatment In one section, the first minute after 24 hours. Also, in the section irradiated with 465 nm, there was no significant decrease until 24 hours, but after 48 hours irradiation, the decrease was significant.
From the above results, it can be seen that the blue LED irradiation method of 405 nm and 465 nm exhibits a superior deactivation effect of Sukutika compared with formalin, which is an existing method.
Example 3: Observation of changes in the interior of Sukitika ward according to LED wavelength
3-1. Observation of scuticacid killing and size granules
3-1-1. How to measure
In order to confirm the change in the inside of the squid cell by the LED wavelength irradiation, the squid cell was suspended in P2Y1 medium at a concentration of 10 5 and 10 6 cells / ml, 10 ml was inoculated into the T25 flask, and 405 nm and 465 nm Wavelength blue light. After the irradiation, 1 ml of the culture solution was dispensed into the E-tube at 3, 6, 12, 24, and 48 hours, and centrifuged at 500 g for 5 minutes. The supernatant was removed and then diluted with phosphate buffered saline (Phosphate Buffered Saline , PBS, pH 7.2-7.4). Subsequently, PI-solution was prepared by diluting Propidium Iodine (PI) to a concentration of 5 μg / μl in PBS, and then 100 μl of the PI-solution was inoculated into an E-tube and reacted at 20 ° C for 15 minutes . The reacted cells were observed for PI staining, size, and complexity using a flow cytometer. After treatment of the same concentration of Sukutika saccharum at 95 ° C for 15 minutes with a positive control (positve control), the same PI staining was performed and compared.
3-1-2. Measurement result
Fig. 4 shows the change in the size of the Sukutika cell according to the LED wavelength irradiation using the flow cytometer. In addition, the ratio of PI + cells is shown in FIG. 5 by reading red fluorescence and gating to observe whether or not the scutica is killed. Because PI is stained only in dead cells and emits red fluorescence, only dead cells can be selected using FL-2, which reads the degree of red fluorescence of cells.
(B): ratio of relative contraction cells = FSC Contraction-treated group / FSC Contraction - control
Expression (c): ratio of relative apoptotic cells = PI + Treated group / PI + Positive control group
The distribution ratios of the relative contraction cells using the above formula (b) are shown in Table 1, and the ratios of the relative apoptotic cells using the above formula (c) are shown in Table 2. .
As shown in Fig. 4 and Tables 1 and 2, when the blue LED was irradiated to the scutica charcoal, cell shrinkage occurred compared with that of the control group scutica. Especially, at 405nm wavelength, the percentage of FSC contraction group cells was more than 140% after 24 hours irradiation, and the proportion of dead cells tended to increase gradually with time.
However, interestingly 10 6 cells / ㎖ the yeoteuna 46.9% when the relative PI +
3-2. Measurement of intracellular reactive oxygen
3-2-1. How to measure
Inoculated Surgical urticae charge after the P2Y1 rich medium at a concentration of 5 × 10 5 cells / ㎖, T25 flask, which was then irradiated with blue light of 405nm and 465nm wavelength. 5 × 10 4 cells were collected at 3, 6, 12, 24, and 48 hours after the irradiation, and the cells were placed in an E-tube, centrifuged at 500 g for 5 minutes, and the supernatant was removed. Subsequently, the pelletized Sutuca pupae were mixed with 100 μl of nitroblue tetrazolium (NBT) (1 mg / ml P2Y1 medium), reacted at 20 ° C for 30 minutes, and then washed with P2Y1 medium Respectively. After washing, the cells were fixed with 100% methyl alcohol for 10 minutes and then washed twice with 70% methyl alcohol. Then, the cells were resuspended in 100 μl of 70% methyl alcohol and inoculated into 96-well plates, then completely dried, and the absorbance was measured at 630 nm by adding 120 μl 2 M KOH and 140 μl of dimethyl sulfoxide.
3-2-2. Measurement result
Fig. 6 shows the degree of active oxygen generated in the sutucca filament according to the LED wavelength irradiation using the following formula (d).
Expression (d): Measurement of active oxygen production amount in the cell = (absorbance at 630 nm Treated group / Absorbance at 630 nm Control group )
As shown in FIG. 6, in the 405 nm wavelength irradiation period, significant increase in reactive oxygen species was observed from 3 hours after the irradiation, and significant increase in reactive oxygen species was observed in the irradiation wavelength of 465 nm from 6 hours. The above results indicate that when the LED of the effective wavelength is directly irradiated to the squid, the amount of active oxygen generated in the parasite is larger than that of the control.
Example 4. Effect of Hanskutika on LED light intensity
4-1. How to measure
500 μl of Sucutica infestation (10 3 cells / ml P2Y1 medium) was inoculated in 12 wells of a 48-well plate and incubated at a wavelength of 405 nm regulated at 250, 185, 99, 37 and 20 mol / 248, 146, and 80 nm / ms, respectively. Thereafter, the time at which all of the 10 3 cells / well of the scutica sacrifice loses motility was observed at 30 min 2 n (n = 0, 1, 2, 3 ...) hours and the result was observed at half the lethal dose LD 50 values.
4-2. Measurement result
The degree of deactivation of the Sutica cord according to the amount of LED light is shown in Fig.
As shown in Figure 7, 405nm (R 2 = 0.984 ) and 465nm (R 2 = 0.9438) was found need more irradiation time as power in order to get the same sterilization efficiency all the more reduced the amount of light irradiation section. From the above results, it can be seen that irradiating a high light quantity to deactivate the scutica plug is more effective than raising the irradiation time.
Example 5. Effects of Huskutikka on the concentration of Sukutika
5-1. How to measure
5-2. Measurement result
As shown in FIG. 8, as the concentration of the Suticaca larvae increased, the time required to kill all of them increased gradually, and the degree of the increase was found to be an exponential relationship (R 2 = 0.9887).
Example 6. Results of biological experiments on flounder
6-1. Effect of LED wavelength on Hansukutika
6-1-1. How to measure
5 * 10 < 6 > cells of Epithelioma Papulosum Cyprini (EPC), a cell line derived from carp epithelium, were collected from carp and then cultured in Leibovitz-15 medium containing 10% FBS and 1% penicillin- streptomycin solution , Cultured at 20 ° C for 3 days, and then used for infecting Sucutika infections in flounder.
The flounder (Paralichthys olicaceus, average weight = 28 ± 6.82g) of Surgical urticae charge (M. avidus) for more than a week after acclimation in 200L water bath, water of a 10ℓ diluted with one-third being held at 20 ℃ 10 3 Cells / ml, and 30 flounders were immersed for 1 hour. After the attack, the cells were housed in a water tank of 40 × 30 × 30 cm and irradiated with LEDs or natural light (control group) at 405 nm (light intensity used = 250 mol / ms) and 465 nm (light intensity used = 516 mol / ms) for 12 hours a day. At this time, the salinity was continuously controlled to 1/3 of the existing seawater and the cumulative mortality rate was observed for 24 days while the water temperature was maintained at 20 ° C.
6-1-2. Measurement result
The cumulative mortality rate of the flounder infected with scuticacidosis is shown in Fig. 9, and the image of the flounder on
As shown in FIG. 9, 100% of the flounder was killed by scuticacid infection in the natural light (control group) for 24 days, and 30% and 70% of the 405 nm and 465 nm wavelength were irradiated, respectively, 465nm wavelength group showed 70% and 30% relative survival rate compared to the control group, respectively.
In addition, as shown in Fig. 10, in the 405nm wavelength region, the ulcer formation by scuticchaemia was found to be very slight compared to the control group. The 465nm wavelength region was not as severe as the control group, but the 405nm wavelength And more severe symptoms. From the above results, it can be seen that the 405 nm wavelength irradiation period is a very effective wavelength range for treating and preventing scuticacidosis.
6-2. Antiparasitic effect according to photoperiod
6-2-1. How to measure
The flounder (average weight = 15 ± 4.40 g) was allowed to pass for more than one week in a 200 L water tank maintained at 20 ° C, and then 10 ℓ of seawater diluted 1/3 was added to 10 3 cells / ml , And the flounder was immersed for 1 hour. (6L: 18D), 12-hour irradiation period (12L: 12D), 18-hour irradiation period (18L: 6D), and 6-hour irradiation period The 24-hour irradiation interval (24L: 0D) and the natural light irradiation interval (12L: 12D) were measured at the maximum light quantity.
6-2-2. Measurement result
Figure 11 shows the cumulative mortality of flounder infested with Sututika after irradiating 405nm LED light with 6L: 18D, 12L: 12D, 18L: 6D, 24L: 0D light period. Where L represents the light irradiation time and the D irradiation time.
As shown in Fig. 11, 24 days after the irradiation, the control group showed a mortality rate of 67%, 6L:
Claims (12)
Wherein the LED light source is maintained at an amount of light in the range of 20 to 1500 占 mol m 占 퐉 -2占 퐏 -1 .
The fishes include flounder, blowfish, carp, cilia, eel, trout, mackerel, mackerel, limoney, flounder, ten gang, strong legs, rockfish, uroguardia, sardine, pollock, codfish, saury, herring, eel, sardine, salmon, tuna Wherein the fish is at least one member selected from the group consisting of anchovy, bonito, anchovy, salmon, red sea bream, and early fish.
Characterized in that the fish comprises a sutucca insect at a concentration of 1 to 1 x 10 8 cells / ml.
Wherein said fish is infected with early scutichosis. ≪ RTI ID = 0.0 > 11. < / RTI >
A method for the prevention or treatment of scutchikosis of fish, characterized in that active oxygen is generated in the sututa crab when an LED light source of blue light is irradiated to the fish.
Characterized in that the fish is treated with a photosensitizer and then irradiated with an LED light source of blue light.
Wherein the photosensitizing substance is selected from the group consisting of indocyanine green, methylene blue, toluidine blue, aminolevulinic acid, phthalocyanine, porphyrin, texapyrine, bacterioclomer, merocyanine, psoralen, benzoporphyrin derivatives and sodium formamide A method for preventing or treating scutchikosis in a fish, the method comprising the steps of:
Wherein the underwater environment is a fish tank, an aquarium, a farm, a pond, a breeding ground, or a marine.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020160022617A KR101800810B1 (en) | 2016-02-25 | 2016-02-25 | Method for prevention and treatment for scuticociliatosis in fishes using blue light emitting diode(LED) |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020160022617A KR101800810B1 (en) | 2016-02-25 | 2016-02-25 | Method for prevention and treatment for scuticociliatosis in fishes using blue light emitting diode(LED) |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20170100266A KR20170100266A (en) | 2017-09-04 |
KR101800810B1 true KR101800810B1 (en) | 2017-11-24 |
Family
ID=59924324
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020160022617A KR101800810B1 (en) | 2016-02-25 | 2016-02-25 | Method for prevention and treatment for scuticociliatosis in fishes using blue light emitting diode(LED) |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR101800810B1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20190127223A (en) | 2018-05-04 | 2019-11-13 | 한국해양대학교 산학협력단 | Aquaculture method using a reducing stress and enhancing immunity by irradiation of light emitting diode in the shrimp |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102372100B1 (en) | 2019-01-22 | 2022-03-10 | 단국대학교 천안캠퍼스 산학협력단 | Method of Removing Contaminant During Solid Fermentation of Tacrolimus Producing Strain Using Blue Light Emitting Diodes |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007252358A (en) * | 2006-03-23 | 2007-10-04 | Akira Yamaoka | Method for breeding fish etc. by using led light |
US20080166304A1 (en) * | 2004-09-17 | 2008-07-10 | The General Hospital Corpoartion | Inactivation of Microorganisms With Multidrug Resistance Inhibitors and Phenothiaziniums |
-
2016
- 2016-02-25 KR KR1020160022617A patent/KR101800810B1/en active IP Right Grant
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080166304A1 (en) * | 2004-09-17 | 2008-07-10 | The General Hospital Corpoartion | Inactivation of Microorganisms With Multidrug Resistance Inhibitors and Phenothiaziniums |
JP2007252358A (en) * | 2006-03-23 | 2007-10-04 | Akira Yamaoka | Method for breeding fish etc. by using led light |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20190127223A (en) | 2018-05-04 | 2019-11-13 | 한국해양대학교 산학협력단 | Aquaculture method using a reducing stress and enhancing immunity by irradiation of light emitting diode in the shrimp |
Also Published As
Publication number | Publication date |
---|---|
KR20170100266A (en) | 2017-09-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP3720710B2 (en) | Novel uses of δ-aminolevulinic acid for the prevention and treatment of fish pathogenic microorganisms and parasites {Noveluseofdelta-aminolevulinicacidforventionation and treatmentofiftificationbypathogeneticcroorganismandparasitic} | |
ES2778844T3 (en) | Complemented fish feed | |
Middlemiss et al. | Combined effects of UV irradiation, ozonation, and the probiotic Bacillus spp. on growth, survival, and general fitness in European lobster (Homarus gammarus) | |
CA2702811A1 (en) | Natural photodynamic agents and their use | |
JP2008044862A (en) | Method for treating and preventing scuticociliatosis in fishes | |
Roh et al. | Blue light-emitting diode light at 405 and 465 nm can inhibit a Miamiensis avidus infection in olive flounder, Paralichthys olivaceus | |
DK202170358A1 (en) | Composition for removing crustacean ectoparasites from farmed salmonid fish using curcumin or a pharmaceutical acceptable derivate thereof and light | |
CN111067007A (en) | Method for killing salmonella through photodynamic | |
KR101800810B1 (en) | Method for prevention and treatment for scuticociliatosis in fishes using blue light emitting diode(LED) | |
Owatari et al. | Amyloodiniosis in farmed Sardinella brasiliensis (Steindachner, 1879): Case report of successful therapeutic control with copper sulfate | |
Linh et al. | Development of mud crab crablet, the identification of ciliates and the bioefficacy of leaf extract of Rhizophora apiculata as anti-protozoal agent | |
CN102349543B (en) | Application of calcined shell/nanometer Cu2O composite | |
EP1836901A1 (en) | Method for producing a feed comprising Artemia nauplii | |
CN106727471A (en) | Inositol as Florfenicol synergist application | |
KR100373147B1 (en) | Treatment agent of Scuticocilictida | |
JP2016163558A (en) | Feed for aquatic animals, growth improving agents for aquatic animals, and methods for rearing cultured fishes using the same feed and agents | |
Li et al. | Effects of sunlamp-based lighting mode on growth performance, survival rate, stress response, and oxidative stress of juvenile Chinese soft-shelled turtles (Pelodiscus sinensis) in a greenhouse | |
CN113100128B (en) | Ultraviolet irradiation device for preventing and treating cryptocaryon irritans disease of marine fishes and use method | |
CN108837029A (en) | A kind of pharmaceutical composition and preparation method thereof preventing and treating saprolegniasis of aquatic animals | |
CN113367099B (en) | Cultivation method and application of coccidiosis-free experimental pigeon | |
KR100451906B1 (en) | A remedy for prevention against fish disease, reinforcement of immune vitality and promotion of growth in a fish farm based on active component of chitosan and oligosaccharide | |
Foroutan et al. | The effects of razor clam (Solen vagina) acetone extract on immunity parameters and bacterial disease resistance in Litopenaeus vannamei. | |
Hartami et al. | Leaf extract effectiveness of pecut kuda (Stachytarpheta jamaicensis L. Vahl) on barramundi (Lates calcarifer) eggs hatchability | |
JP2017000085A (en) | Ectoparasite control method for marine pisciculture | |
NO20210775A1 (en) | Method for removing crustacean ectoparasites from farmed salmonid fish using water-soluble tetrapyrrole compounds and light |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant |