CN106361616A - Golden flower fungus, tea and toadstool facial mask and preparation method thereof - Google Patents
Golden flower fungus, tea and toadstool facial mask and preparation method thereof Download PDFInfo
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- CN106361616A CN106361616A CN201610971586.5A CN201610971586A CN106361616A CN 106361616 A CN106361616 A CN 106361616A CN 201610971586 A CN201610971586 A CN 201610971586A CN 106361616 A CN106361616 A CN 106361616A
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- eurotium cristatum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention provides a golden flower fungus, tea and toadstool facial mask. The facial mask is prepared by adding auxiliary materials after fermenting extract liquid of tea leaves and toadstool, particularly golden flower fungus is used for fermentation; on one hand, the moisture preserving effect of the toadstool is improved, the skin color can be improved effectively, particular nutritional ingredients of the toadstool can be absorbed by skin, the skin is prevented from aging effectively, and wrinkles are reduced; on the other hand, the golden flower fungus is fermented, and a plenty of microbial metabolites or various functional healthcare factors of microbial thalli are added, so that the product nutrition is more abundant, the health of people is facilitated, and the facial mask is easily absorbed by the skin; and thirdly, oxyradical removal and antioxidant functions of the particular abundant amino acids and vitamin C of the toadstool are combined with antioxidant and anti-radiation functions of inherent tea polyphenols, theanine and tea polysaccharides in tea, so that the prepared nutrient facial mask has the all-in-one functions of toxicant elimination, human free radical removal, oxidation resistance, radiation resistance and the like.
Description
Technical field
The present invention relates to facial film technical field is and in particular to a kind of eurotium cristatum tea Morchella esculenta (L.) Perss facial film and preparation method thereof.
Background technology
The mankind are used facial film with a long history as cosmetic tool, can trace back to ancient Egypt and Chinese Tang Dynasty.Facial film former
Reason is to be applied in face using the dressing of a thick layer of not same-action and composition, intercepts the contact with air for the skin.When this layer applies
When material tightly pastes on skin, skin temperature can be made to increase because of the secretion of suppression sebum and antiperspirant evaporation of water；Skin temperature
After rising, will blood circulation promoting, so that the nutrient of infiltration skin is broadly spread apart iuntercellular is deeper.Skin surface that
The moisture that cannot evaporate a bit then can be deposited in epidermal area, and the moisture of epidermal area is full, and skin seems will smoother
Tight, microgroove also can become light；In addition, temperature and warm can make cutin softening, capillary bore expansion；Capillary bore expansion, piles up
Sweat mixed with dirt inside just can seize the opportunity exclusion, and therefore facial film can more thoroughly clean skin.
Morchella esculenta (L.) Perss delicious flavour itself, mouthfeel is tender, is high-grade top food materials.The free amino acid wherein containing and
5 '-nucleotide is extremely abundant, have concurrently simultaneously the delicate flavours such as abundant aspartic acid, glutamic acid, glycine, alanine aminoacid and
The nucleotide of the delicate flavours such as 5'-GMP, 5'-inosinic acid.And, the effects such as Morchella esculenta (L.) Perss have supplementing the brain, refresh oneself, not only nutrition is rich
Rich, delicious flavour, and have the health-care effects such as unique whitening, antiinflammatory, smoothing wrinkle.
Eurotium Cristatum is a kind of probiotic bacteria, is distinctive a kind of microorganism in Fu-brick tea, has important work to the quality of Fu-brick tea
With.Research shows that Eurotium Cristatum can produce extracellular polysaccharide, all has effect for suppression tumor cell, lipid-loweringing, additionally, Eurotium Cristatum is sent out
Zymotic fluid scalable digestion function of stomach and intestine, its mycelium contains the essential amino acids of needed by human body.Function to human immune system
Play an important role.
Existing patent cn201610397978.5 discloses a kind of Morchella esculenta (L.) Perss clay mask and preparation method thereof, by following raw material system
Become: Gaster caprae seu Ovis bacterium mud 12-24wt%, wetting agent 4-8wt%, tackifier 0.5-1wt%, metal ion chelation agent 3-10wt%, anti-
Rotten agent 0.05-0.08wt%, brightening agent 2-4wt% and nourishing additive agent 6-10wt%, balance of deionized water.Preparation method
For: first Gaster caprae seu Ovis bacterium mud and deionized water are heated to 35-55 DEG C of insulation 2-3 hour, are subsequently cooled to room temperature and obtain Morchella esculenta (L.) Perss
Mud solution；Wetting agent, metal ion chelation agent, preservative, brightening agent and nourishing additive agent are dissolved in deionized water, Ran Houjia
Enter Gaster caprae seu Ovis bacterium mud solution and continue stirring, add tackifier and deionized water while stirring, after stirring, obtain Gaster caprae seu Ovis bacterium mud face
Film.
How effectively to prepare top cosmetics and skincare product with probiotics fermention so that skin care item back to nature and safety
It is effectively a main trend of current research.
Content of the invention
It is an object of the invention to provide a kind of natural, healthy, nutrition, health care, convenient eurotium cristatum tea Morchella esculenta (L.) Perss facial film, carry
It is effectively increased epiderm skin moisture for one kind, moistening effect is excellent, also there is whitening, antiinflammatory, smoothing wrinkle and the eurotium cristatum tea refreshed oneself
Morchella esculenta (L.) Perss facial film, eurotium cristatum tea Morchella esculenta (L.) Perss facial film be fermented with the lixiviating solution of Folium Camelliae sinensis, Morchella esculenta (L.) Perss after add adjuvant preparation and
Become.
For solving above-mentioned technical problem, the technical solution used in the present invention is as follows:
On the one hand, the present invention provides a kind of eurotium cristatum tea Morchella esculenta (L.) Perss facial film, and eurotium cristatum tea Morchella esculenta (L.) Perss facial film includes Eurotium Cristatum
Tea fermented morel liquid, wetting agent, tackifier, preservative and brightening agent.
Further, the concrete preparation process of described eurotium cristatum tea fermented morel liquid is:
(a) pretreatment: take Morchella esculenta (L.) Perss, go the removal of impurity, clean, use 100 mesh sieve after pulverizing, siftage is added to water
In, add water, homogenizing by the amount of solid-liquid ratio 1:1～5, be heated to 90～100 degrees Celsius of extraction 1～2h, cooling is standby；
B () inoculation liquid is cultivated: the sucrose adding 10%～15% in test tube after sterilization is carbon source, adds Folium Camelliae sinensis, tea
Leaf consumption is 0.5～1% fluid medium, with white vinegar, ph is adjusted to 5～6, high temperature sterilize；Under sterile conditions, connect
Plant Eurotium Cristatum, (spore concentration is 5～6 × 10 to inoculum concentration 1%～5%8Individual/ml), rocking-turn number 120r/min, cultivation temperature is
28～30 degrees Celsius, cultivate 3 days；
C culture fluid aseptic water washing bacterium colony surface that step (b) has been inoculated by (), prepares Eurotium Cristatum spore suspension
(spore concentration is 5～6 × 108Individual/ml), stand-by；
D () feeds: the Morchella esculenta (L.) Perss handled well aqueous solution is placed in solid-state fermentation tank, stocking volume is 30%～85%,
After being cooled down with 100～120 degrees Celsius of steam high temperature sterilizes, by the Eurotium Cristatum spore suspension of preparation, (spore concentration is 5～6
×108Individual/ml) uniformly add in solid-state fermentation tank, suitably mix；
E () is fermented: charged solid-state fermentation tank is sealed, the temperature of fermentation tank is maintained at 22～38 degrees Celsius, ph is 3
Under conditions of～6, after the sweat of 5～10 days, filter, collect filtrate, obtain final product eurotium cristatum tea fermented morel liquid.
Further, the temperature that described step (e) is fermented is maintained at 28～30 degrees Celsius, and ph is 5.
Morchella esculenta (L.) Perss of the present invention can be fresh collection or dry Morchella esculenta (L.) Perss.
Further, in described facial film, the number of each component is 85～95 parts of eurotium cristatum tea fermented morel liquid, wetting agent 4
～8 parts, 0.5～1 part of tackifier, 0.05～0.08 part of preservative and 2～4 parts of brightening agent.
Further, described wetting agent is the group of one or more of glycerol, propylene glycol, butanediol and hyaluronate sodium
Close.
Further, described tackifier is the combination of one of high molecular cellulose and HANSHENGJIAO or two kinds.
Further, described preservative is the combination of one of phenoxyethanol or Sang Pu k15 or two kinds.
Further, described brightening agent is the combination of one of arbutin, nicotiamide and Trenaxmine or two kinds.
Further, described Eurotium Cristatum is Eurotium cristatum, and deposit number is the bacterial strain of cgmcc3.2167.
Further, described Folium Camelliae sinensis are green tea, black tea, postfermented tea or oolong tea.
On the one hand, the present invention provides a kind of method preparing eurotium cristatum tea Morchella esculenta (L.) Perss facial film, and concrete preparation process is as follows:
Will be soluble in water to wetting agent, preservative and brightening agent, it is subsequently adding eurotium cristatum tea fermented morel liquid and stir,
Add tackifier and water while stirring, after stirring, obtain eurotium cristatum tea Morchella esculenta (L.) Perss facial film.
On the other hand, the present invention provides a kind of method preparing eurotium cristatum tea Morchella esculenta (L.) Perss facial film, and concrete preparation process is as follows:
1) will be soluble in water to wetting agent, preservative and brightening agent, it is subsequently adding the stirring of eurotium cristatum tea fermented morel liquid all
Even, add tackifier and water while stirring, mix to obtain solution；
2), in solution and then by Biofibre facial mask soaking step 1), fully absorb after Biofibre facial mask and take after solution
Go out, obtain final product eurotium cristatum tea Morchella esculenta (L.) Perss facial film.
Further, described Eurotium Cristatum is Eurotium cristatum (eurotium cristatum).
Further, the deposit number of described Eurotium Cristatum is the bacterial strain of cgmcc3.2167.
Further, described Biofibre facial mask is the corpus fibrosum being made up of acetobacter xylinum natural fermentation.
Biofibre facial mask is ultra-thin because of it, and cooperation skin maintenance material is docile with skin super, is attached to and seems do not have on the face
As thing, as script, fit tightly skin, elastic force compacts lifting, and compact skin, strengthening absorbs.
The facial film of the present invention has the effect that
The present invention provide eurotium cristatum tea Morchella esculenta (L.) Perss facial film be fermented with the lixiviating solution of Folium Camelliae sinensis, Morchella esculenta (L.) Perss after add auxiliary
Material is prepared from, and is fermented especially with Eurotium Cristatum, has on the one hand both improved the moistening effect of Morchella esculenta (L.) Perss, and colour of skin energy
The exclusive nutritional labeling of enough effectively improvement, Morchella esculenta (L.) Perss itself can effectively be prevented skin aging, reduce wrinkle by percutaneous absorption
Stricture of vagina；Another aspect Eurotium Cristatum ferments, and adds the several functions guarantor that many microbe metabolites or microbial cells have in itself
The strong factor, product nutrition is more rich, favorably with the health of human body it is easy to be absorbed by the skin；3rd, exclusive rich of Morchella esculenta (L.) Perss itself
The aminoacid of richness, the removing oxyradical of vitamin c and anti-oxidation function, in conjunction with intrinsic tea polyphenols in tea, theanine, tea
The antioxidation of polysaccharide and radiation-resisting functional are so that be prepared into many with toxin expelling, removing human free radical, antioxidation, radioprotective etc.
The nourishing pack of position one body function.
The eurotium cristatum tea Morchella esculenta (L.) Perss facial film of the life-time service present invention, has functions that anticancer, enhance immunity, to refreshing oneself,
Toxin expelling, whitening, moisturizing, slow down aging etc. have multi-efficiency.The facial film of the present invention, safe and non-stimulating, for a long time can be safe to use,
For sensitivity, skin crowd is also suitable.
The preparation method of the eurotium cristatum tea Morchella esculenta (L.) Perss facial film that the present invention provides, raw material sources are simple, and sweat is easy to control
System, is conducive to control and the popularization of product quality.The facial film preparation method of the present invention is convenient and simple quick, can be suitable for various scales
Quick production, can be effectively cost-effective.
Specific embodiment
With specific embodiment, the present invention is further illustrated below, but the present invention does not accept the restriction stating embodiment.
Embodiment 1
Preparation eurotium cristatum tea Morchella esculenta (L.) Perss facial film, concrete preparation process is as follows:
(a) pretreatment: take dry Morchella esculenta (L.) Perss, go the removal of impurity, clean, use 100 mesh sieve after pulverizing, siftage is added to
In water, add water, homogenizing by the amount of feed liquid mass ratio 1:5, be heated to 100 degrees Celsius of extraction 2h, cooling is standby；
B () inoculation liquid is cultivated: the sucrose adding 10% in test tube after sterilization is carbon source, adds green tea, Folium Camelliae sinensis
Consumption is 0.8% fluid medium, with white vinegar, ph is adjusted to 5.5, high temperature sterilize；Under sterile conditions, inoculate JINHUA
Bacterium, (spore concentration is 5～6 × 10 to inoculum concentration 1%8Individual/ml), rocking-turn number 120r/min, cultivation temperature is that 28-30 is Celsius
Degree, cultivates 3 days；
C culture fluid aseptic water washing bacterium colony surface that step (b) has been inoculated by (), prepares Eurotium Cristatum spore suspension
(spore concentration is 5～6 × 108Individual/ml), stand-by；
D () feeds: the Morchella esculenta (L.) Perss handled well aqueous solution is placed in solid-state fermentation tank, stocking volume is 85%, with 100～
After 120 degrees Celsius of steam high temperature sterilize cooling, by the Eurotium Cristatum spore suspension of preparation, (spore concentration is 5～6 × 108Individual/
Ml) in uniform addition solid-state fermentation tank, suitably mix；
E () is fermented: charged solid-state fermentation tank is sealed, the temperature of fermentation tank is maintained at 32～38 degrees Celsius, ph is 6
Under conditions of, after the sweat of 5 days, filter, collect filtrate, obtain final product eurotium cristatum tea fermented morel liquid；
F prepared by () facial film: 4 parts of wetting agent, 0.05 part of preservative and 2 parts of brightening agent are dissolved in 5 parts of water, are subsequently adding step
Suddenly 85 parts of the eurotium cristatum tea fermented morel liquid of (e) stirs, and adds 0.5 part and 1 part water of tackifier while stirring, and stirring is all
Eurotium cristatum tea Morchella esculenta (L.) Perss facial film is obtained after even；
Described wetting agent is the combination of glycerol and hyaluronate sodium, and mass ratio is 1:1；Described tackifier is macromolecular fibre
Element；Described preservative is phenoxyethanol；Described brightening agent is the combination of arbutin and Trenaxmine, and mass ratio is 2:1.
Embodiment 2
Preparation eurotium cristatum tea Morchella esculenta (L.) Perss facial film, concrete preparation process is as follows:
(a) pretreatment: take fresh Morchella esculenta (L.) Perss, go the removal of impurity, clean, use 100 mesh sieve after pulverizing, siftage adds
To in water, add water, homogenizing by the amount of feed liquid mass ratio 1:1, be heated to 90～100 degrees Celsius of extraction 1h, cooling is standby；
B () inoculation liquid is cultivated: the sucrose adding 15% in test tube after sterilization is carbon source, adds black tea leaf, Folium Camelliae sinensis
Consumption is 0.5% fluid medium, with white vinegar, ph is adjusted to 5, high temperature sterilize；Under sterile conditions, inoculate Eurotium Cristatum,
(spore concentration is 5～6 × 10 to inoculum concentration 5%8Individual/ml), rocking-turn number 120r/min, cultivation temperature is 28-30 degree Celsius, training
Support 3 days；
C culture fluid aseptic water washing bacterium colony surface that step (b) has been inoculated by (), prepares Eurotium Cristatum spore suspension
(spore concentration is 5～6 × 108Individual/ml), stand-by；
D () feeds: the Morchella esculenta (L.) Perss handled well aqueous solution is placed in solid-state fermentation tank, stocking volume is 30%, with 100～
After 120 degrees Celsius of steam high temperature sterilize cooling, by the Eurotium Cristatum spore suspension of preparation, (spore concentration is 5～6 × 108Individual/
Ml) in uniform addition solid-state fermentation tank, suitably mix；
E () is fermented: charged solid-state fermentation tank is sealed, the temperature of fermentation tank is maintained at 22～30 degrees Celsius, ph is 3
Under conditions of, after the sweat of 10 days, filter, collect filtrate, obtain final product eurotium cristatum tea fermented morel liquid；
F prepared by () facial film: 8 parts of wetting agent, 0.08 part of preservative and 4 parts of brightening agent are dissolved in 8 parts of water, are subsequently adding step
Suddenly 95 parts of the eurotium cristatum tea fermented morel liquid of (e) stirs, and adds 1 part and 2 parts water of tackifier while stirring, stirs
Afterwards by Biofibre facial mask soak, fully absorb after Biofibre facial mask and take out after solution, obtain eurotium cristatum tea Morchella esculenta (L.) Perss
Facial film；
Described wetting agent is the combination of propylene glycol, butanediol and hyaluronate sodium, and mass ratio is 0.5:1:1；Described adhesion-promoting
Agent is the combination of high molecular cellulose and HANSHENGJIAO, and mass ratio is 0.5:1；Described preservative is Sang Pu k15；Described brightening agent is
Nicotiamide and the combination of Trenaxmine, mass ratio is 1:3.
Embodiment 3
Preparation eurotium cristatum tea Morchella esculenta (L.) Perss facial film, concrete preparation process is as follows:
(a) pretreatment: take dry Morchella esculenta (L.) Perss, go the removal of impurity, clean, use 100 mesh sieve after pulverizing, siftage is added to
In water, add water, homogenizing by the amount of solid-liquid ratio 1:3, be heated to 90～100 degrees Celsius of extraction 1.5h, cooling is standby；
B () inoculation liquid is cultivated: the sucrose adding 13% in test tube after sterilization is carbon source, adds frozen fresh oolong tea, tea
Leaf consumption is 1% fluid medium, with white vinegar, ph is adjusted to 6, high temperature sterilize；Under sterile conditions, inoculate Eurotium Cristatum,
(spore concentration is 5～6 × 10 to inoculum concentration 3%8Individual/ml), shaking flask revolution 120r/min, cultivation temperature is 28～30 degrees Celsius,
Culture 3 days；
C culture fluid aseptic water washing bacterium colony surface that step (b) has been inoculated by (), prepares Eurotium Cristatum spore suspension
(spore concentration is 5～6 × 108Individual/ml), stand-by；
D () feeds: the Morchella esculenta (L.) Perss handled well aqueous solution is placed in solid-state fermentation tank, stocking volume is 30%～85%,
After being cooled down with 100～120 degrees Celsius of steam high temperature sterilizes, by the Eurotium Cristatum spore suspension of preparation, (spore concentration is 5～6
×108Individual/ml) uniformly add in solid-state fermentation tank, suitably mix；
E () is fermented: charged solid-state fermentation tank is sealed, the temperature of fermentation tank is maintained at 28～30 degrees Celsius, ph is 5
Under conditions of, after the sweat of 7 days, filter, collect filtrate, obtain final product eurotium cristatum tea fermented morel liquid；
F prepared by () facial film: 5 parts of wetting agent, 0.06 part of preservative and 3 parts of brightening agent are dissolved in 2 parts of water, are subsequently adding step
Suddenly 90 parts of the eurotium cristatum tea fermented morel liquid of (e) stirs, and adds 0.8 part and 0.5 part water of tackifier, stirring while stirring
Eurotium cristatum tea Morchella esculenta (L.) Perss facial film is obtained after uniformly；
Described wetting agent is glycerol；Described tackifier is HANSHENGJIAO；Described preservative is the group of phenoxyethanol and Sang Pu k15
Close, mass ratio is 4:1；Described brightening agent is Trenaxmine.
Embodiment 4
Eurotium cristatum tea Morchella esculenta (L.) Perss facial film performance of keeping humidity is tested:
Weigh eurotium cristatum tea Morchella esculenta (L.) Perss facial film, blank and the positive are made respectively with the glycerine water solution of distilled water and 15%
Comparison, solution is respectively placed in tared dish, uncovered placement in 25 DEG C of temperature, the climatic chamber of relative humidity 30%, places
Time is 12,24,36h, sample is taken out together with weighing botle and weighs, calculate its moisturizing rate, test parallel 3 groups.
Moisturizing rate=m/m0× 100%
In formula: m is the gross mass of tared dish before placement；m0Gross mass for tared dish after placing.
The experimental result of eurotium cristatum tea Morchella esculenta (L.) Perss facial film, distilled water and 15% glycerine water solution is specifically as shown in table 1,
Table 1: eurotium cristatum tea Morchella esculenta (L.) Perss facial film performance of keeping humidity test
Knowable to result shown in from table 1, eurotium cristatum tea Morchella esculenta (L.) Perss facial film, after 36 hours, still has preferable moisturizing
Effect, its moisturizing rate reaches 70%.
Embodiment 5
B16 cell experiment in suppression mouse melanin cell
B16 mouse melanin tumor cell is inoculated in culture dish with the density of 1 × 80/ml, in 37 DEG C of 5%co2Bar
Cultivate 24h under part, abandon supernatant, add eurotium cristatum tea fermented morel liquid 18～22ml, continue culture 36h, abandon supernatant,
Pbs washs, and every hole adds the trypsin solution peptic cell 3min of 0.5ml mass percent 0.2%, plus 2ml maintaining liquid terminates disappearing
Change.After mixing, take out 0.5ml and make cell counting, remaining cell suspension is centrifuged 5min with 2500r/min, abandons supernatant, to precipitation
Middle addition 1m naoh solution 0.5ml, heating makes melanin dissolve, and selects to survey extinction on the enzyme-linked immunosorbent assay instrument of 490nm wavelength
Angle value.
B16 cell suppression ratio=(1- (medicine hole absorbance+medicine hole cell density)+(control wells absorbance+right
According to hole cell density)) × 100%.Experimental result is as shown in table 2:
B16 cell experiment in table 2 suppression mouse melanin cell
As known from Table 2, the eurotium cristatum tea Morchella esculenta (L.) Perss facial film of present invention preparation can effectively suppress melanin to generate, and effect shows
Write.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, made any repair
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of eurotium cristatum tea Morchella esculenta (L.) Perss facial film is it is characterised in that described eurotium cristatum tea Morchella esculenta (L.) Perss facial film includes eurotium cristatum tea sheep
Tripe fermented liquid, wetting agent, tackifier, preservative and brightening agent.
2. eurotium cristatum tea Morchella esculenta (L.) Perss facial film according to claim 1 it is characterised in that
The concrete preparation process of described eurotium cristatum tea fermented morel liquid is:
(a) pretreatment: take Morchella esculenta (L.) Perss, go the removal of impurity, clean, use 100 mesh sieve after pulverizing, siftage is added to the water, and presses
The amount of solid-liquid ratio 1:1～5 adds water, homogenizing, is heated to 90～100 degrees Celsius of extraction 1～2h, and cooling is standby；
B () inoculation liquid is cultivated: the sucrose adding 10%～15% in test tube after sterilization is carbon source, adds Folium Camelliae sinensis, Folium Camelliae sinensis are used
Measure the fluid medium for 0.5～1%, with white vinegar, ph is adjusted to 5～6, high temperature sterilize；Under sterile conditions, inoculate gold
Flower bacterium, (spore concentration is 5～6 × 10 to inoculum concentration 1%～5%8Individual/ml), shaking flask revolution 120r/min, cultivation temperature be 28～
30 degrees Celsius, cultivate 3 days；
C culture fluid aseptic water washing bacterium colony surface that step (b) has been inoculated by (), prepares Eurotium Cristatum spore suspension (spore
Concentration is 5～6 × 108Individual/ml), stand-by；
D () feeds: the Morchella esculenta (L.) Perss handled well aqueous solution is placed in solid-state fermentation tank, stocking volume is 30%～85%, uses 100
After～120 degrees Celsius of steam high temperature sterilize cooling, by the Eurotium Cristatum spore suspension of preparation, (spore concentration is 5～6 × 108
Individual/ml) uniformly add in solid-state fermentation tank, suitably mix；
E () is fermented: charged solid-state fermentation tank is sealed, the temperature of fermentation tank is maintained at 22～38 degrees Celsius, ph is 3～6
Under conditions of, after the sweat of 5～10 days, filter, collect filtrate, obtain final product eurotium cristatum tea fermented morel liquid.
3. eurotium cristatum tea Morchella esculenta (L.) Perss facial film according to claim 2 is it is characterised in that the temperature fermented of described step (e)
Degree is maintained at 28～30 degrees Celsius, and ph is 5.
4. eurotium cristatum tea Morchella esculenta (L.) Perss facial film according to claim 1 it is characterised in that in described facial film each component number
For 85～95 parts of eurotium cristatum tea fermented morel liquid, 4～8 parts of wetting agent, 0.5～1 part of tackifier, 0.05～0.08 part of preservative
With 2～4 parts of brightening agent.
5. eurotium cristatum tea Morchella esculenta (L.) Perss facial film according to claim 1 it is characterised in that described wetting agent be glycerol, the third two
The combination of one or more of alcohol, butanediol and hyaluronate sodium.
6. eurotium cristatum tea Morchella esculenta (L.) Perss facial film according to claim 1 is it is characterised in that described tackifier is macromolecular fibre
The combination of element and one of HANSHENGJIAO or two kinds.
7. eurotium cristatum tea Morchella esculenta (L.) Perss facial film according to claim 1 it is characterised in that described preservative be phenoxyethanol or
The combination of one of Sang Pu k15 or two kinds.
8. eurotium cristatum tea Morchella esculenta (L.) Perss facial film according to claim 1 is it is characterised in that described brightening agent is arbutin, cigarette
The combination of one of amide and Trenaxmine or two kinds.
9. a kind of method preparing eurotium cristatum tea Morchella esculenta (L.) Perss facial film is it is characterised in that concrete preparation process is as follows:
Will be soluble in water to wetting agent, preservative and brightening agent, it is subsequently adding eurotium cristatum tea fermented morel liquid and stirs, while stirring
Mix side and add tackifier and water, after stirring, obtain eurotium cristatum tea Morchella esculenta (L.) Perss facial film；
The concrete preparation process of described eurotium cristatum tea fermented morel liquid is:
(a) pretreatment: take Morchella esculenta (L.) Perss, go the removal of impurity, clean, use 100 mesh sieve after pulverizing, siftage is added to the water, and presses
The amount of solid-liquid ratio 1:1～5 adds water, homogenizing, is heated to 90～100 degrees Celsius of extraction 1～2h, and cooling is standby；
B () inoculation liquid is cultivated: the sucrose adding 10～15% in test tube after sterilization is carbon source, adds Folium Camelliae sinensis, Folium Camelliae sinensis consumption
Ph is adjusted to 5～6 with white vinegar, high temperature sterilize by the fluid medium for 0.5～1%；Under sterile conditions, inoculate JINHUA
Bacterium, (spore concentration is 5～6 × 10 to inoculum concentration 1～5%8Individual/ml), shaking flask revolution 120r/min, cultivation temperature is taken the photograph for 28-30
Family name's degree, cultivates 3 days；
C culture fluid aseptic water washing bacterium colony surface that step (b) has been inoculated by (), prepares Eurotium Cristatum spore suspension (spore
Concentration is 5～6 × 108Individual/ml), stand-by；
D () feeds: the Morchella esculenta (L.) Perss handled well aqueous solution is placed in solid-state fermentation tank, stocking volume is 30%～85%, uses 100
After～120 degrees Celsius of steam high temperature sterilize cooling, by the Eurotium Cristatum spore suspension of preparation, (spore concentration is 5～6 × 108
Individual/ml) uniformly add in solid-state fermentation tank, suitably mix；
E () is fermented: charged solid-state fermentation tank is sealed, the temperature of fermentation tank is maintained at 22～38 degrees Celsius, ph is 3～6
Under conditions of, after the sweat of 5～10 days, filter, collect filtrate, obtain final product eurotium cristatum tea fermented morel liquid.
10. a kind of method preparing eurotium cristatum tea Morchella esculenta (L.) Perss facial film is it is characterised in that concrete preparation process is as follows:
1) will be soluble in water to wetting agent, preservative and brightening agent, it is subsequently adding eurotium cristatum tea fermented morel liquid and stir, side
Stirring side adds tackifier and water, mixes to obtain solution；
2), in solution and then by Biofibre facial mask soaking step 1), fully absorb after Biofibre facial mask and take out after solution,
Obtain final product eurotium cristatum tea Morchella esculenta (L.) Perss facial film；
The concrete preparation process of described eurotium cristatum tea fermented morel liquid is:
(a) pretreatment: take Morchella esculenta (L.) Perss, go the removal of impurity, clean, use 100 mesh sieve after pulverizing, siftage is added to the water, and presses
The amount of solid-liquid ratio 1:1～5 adds water, homogenizing, is heated to 90～100 degrees Celsius of extraction 1～2h, and cooling is standby；
B () inoculation liquid is cultivated: the sucrose adding 10～15% in test tube after sterilization is carbon source, adds Folium Camelliae sinensis, Folium Camelliae sinensis consumption
Ph is adjusted to 5～6 with white vinegar, high temperature sterilize by the fluid medium for 0.5～1%；Under sterile conditions, inoculate JINHUA
Bacterium, (spore concentration is 5～6 × 10 to inoculum concentration 1～5%8Individual/ml), shaking flask revolution 120r/min, cultivation temperature is taken the photograph for 28-30
Family name's degree, cultivates 3 days；
C culture fluid aseptic water washing bacterium colony surface that step (b) has been inoculated by (), prepares Eurotium Cristatum spore suspension (spore
Concentration is 5～6 × 108Individual/ml), stand-by；
D () feeds: the Morchella esculenta (L.) Perss handled well aqueous solution is placed in solid-state fermentation tank, stocking volume is 30%～85%, uses 100
After～120 degrees Celsius of steam high temperature sterilize cooling, by the Eurotium Cristatum spore suspension of preparation, (spore concentration is 5～6 × 108
Individual/ml) uniformly add in solid-state fermentation tank, suitably mix；
E () is fermented: charged solid-state fermentation tank is sealed, the temperature of fermentation tank is maintained at 22～38 degrees Celsius, ph is 3～6
Under conditions of, after the sweat of 5～10 days, filter, collect filtrate, obtain final product eurotium cristatum tea fermented morel liquid.
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| CN110613655A (en) * | 2019-11-04 | 2019-12-27 | 沈阳农业大学 | Preparation method and application of novel natural facial mask liquid |
| CN114452241A (en) * | 2022-03-16 | 2022-05-10 | 渤海大学 | Probiotic small-molecule facial mask and preparation method thereof |
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| CN109820778A (en) * | 2019-02-20 | 2019-05-31 | 田朝晖 | A kind of hickory chick biofermentation bacterium solution composite skin care product and preparation method thereof |
| CN110613655A (en) * | 2019-11-04 | 2019-12-27 | 沈阳农业大学 | Preparation method and application of novel natural facial mask liquid |
| CN110613655B (en) * | 2019-11-04 | 2022-06-03 | 沈阳农业大学 | Preparation method and application of natural facial mask liquid |
| CN114452241A (en) * | 2022-03-16 | 2022-05-10 | 渤海大学 | Probiotic small-molecule facial mask and preparation method thereof |
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