CN106360036A - Laying duck additive premix for relieving salted egg yolk core hardening and use thereof - Google Patents
Laying duck additive premix for relieving salted egg yolk core hardening and use thereof Download PDFInfo
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Abstract
The invention discloses a laying duck additive premix for relieving salted egg yolk core hardening and a use thereof and relates to the field of feed additive premix production. The laying duck additive premix comprises additives and a carrier. The additives comprise 6-15mg/kg of soy isoflavone and 20-100mg/kg of vitamin E. The carrier is cob powder, zeolite powder or bentonite. The laying duck additive premix is rich in biologically active substances and vitamins, has biological functions of improving a body antioxidation capability, has no toxic or side effect, is beneficial for laying duck laying and human health and is prepared and used through simple and convenient processes. The laying duck additive premix is used for laying duck and laying breeding duck feed, improves a body antioxidation capability, improves duck egg quality, reduces salted egg yolk fat and protein oxidation, promotes salted egg yolk oil production and arenaceous taste formation, reduces salted egg yolk core hardening and is environmentally friendly and safe.
Description
Technical field
The present invention relates to feedstuff additive premix compound production field is and in particular to a kind of laying ducks of alleviation preserved egg yellow hard-core add
Plus agent premix material and its application.
Background technology
In recent years, with the propulsion of China's aquatic bird industrialization process, regular power, protein feed resources shortage, laying ducks
Feedstuff is widely using unconventional water resources to alleviate the feed resources such as Semen Maydiss, bean cake deficiency, reduction feeding cost, the economic effect of raising
While beneficial, quality preserved egg yellow declines year by year.It is mainly manifested in preserved egg yellow hard-core, white heart rate increases, fuel-displaced, dusting
Ratio decline, cause huge economic losses to food service industrys such as the related moon cake of preserved egg yellow, rice tamale, vitellus-pie.Unconventional raise
Material nutrient is unbalanced, and Semen Maydiss and big rice processed side product such as Testa oryzae, Semen Maydiss ddgs class are rich in non-starch polysaccharides(nsp) and how unsaturated
Fatty acid, the miscellaneous dregs of rice such as Sorghum vulgare Pers., rapeseed cake, cottonseed meal because containing some noxious substances and antinutritional factor, using unreasonable can be to poultry machine
Body oxidation balance system adversely affects, and reduces livestock products quality.Studies have reported that in recent years, in food processing process,
Lipid peroxide and its catabolite can be with protein covalent bond, and oxygen-derived free radicals can cause protein oxidation, cause albumen
Between matter molecule or subunit, covalent cross-linking and hydrophobic group expose, and form polymer.
At present, preserved egg yellow maturation mechanism research aspect, existing research both at home and abroad is concentrated mainly on salted egg in curing process
Study point with the change of physical behavior on, be not directed to the aspects such as duck kind, feed formula, feeding manner, processing technique.
Content of the invention
In order to overcome shortcoming and the deficiency of prior art, the primary and foremost purpose of the present invention is to provide a kind of alleviation preserved egg yellow hard
The laying ducks additive premix of the heart.This premix material is efficient, safe and environment-friendly；To improve laying ducks oxidation resistance, to improve Ovum Anas domestica product
Matter, reduction preserved egg yellow hard-core, provide the preserved egg yellow of high-quality for consumer.
Another object of the present invention is to providing the application of the laying ducks additive premix of above-mentioned alleviation preserved egg yellow hard-core.
The purpose of the present invention is achieved through the following technical solutions:
A kind of laying ducks additive premix alleviating preserved egg yellow hard-core, is made up of additive and carrier, and described additive becomes
It is divided into: soybean isoflavone and vitamin e.
The addition of described additive is soybean isoflavone 6～15mg/kg, vitamin e 20～100mg/kg.
Described carrier is maize cob meal, zeolite powder or bentonite；
The laying ducks additive premix of described alleviation preserved egg yellow hard-core is in preparation for laying ducks and meat egg dual-purpose type duck
Application in mixed feed.
The using method of the additive premix of alleviation preserved egg yellow hard-core that the present invention provides, pre- with other feed additives
Batch mixing is similar, this premix material respectively according to 5～7.5kg/ ton, 1～1.5kg/ ton, 200～300g/ ton ratio add to
4% premix material, 20% concentrated feed, in perfect compound feed.
The present invention, with respect to prior art, has such advantages as and effect:
(1) additive premix of the present invention has and has no toxic side effect, is beneficial to laying ducks and lays eggs and human health, and tool
There is preparation and application simply and easily advantage；
(2) additive premix of the present invention is rich in bioactive substance and vitamin, has raising body antioxidation energy
The aspect biological function such as power；
(3) additive premix of the present invention is applied in the feedstuff such as laying ducks and meat egg dual-purpose type duck, machine can be improved
Body oxidation resistance, improves Ovum Anas domestica quality, reduces preserved egg yellow fat and protein oxidation, promotes the fuel-displaced dusting of preserved egg yellow, minimizing
Preserved egg yellow hard-core and other effects, is green, the safe feedstuff additive premix compound of a class.
Brief description
Fig. 1 is the scanning electron microscope (SEM) photograph pickling 50d preserved egg yellow in embodiment 1,500 ×.
Fig. 2 is to pickle the scanning electron microscope (SEM) photograph that 50d boils preserved egg yellow hard-core in embodiment 1,500 ×.
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention do not limit
In this.
Embodiment 1
Laying ducks are used for alleviating the laying ducks additive premix of preserved egg yellow hard-core, and wherein premix material includes following ingredients: containing big
Bean isoflavone 10mg/kg, vitamin e 40mg/kg, the carrier of described premix material is stone powder, zeolite powder or bentonite.Originally raised
Feed additives premix material is added in laying ducks complete feed according to the ratio of 200～300g/ ton.
This feedstuff additive premix compound is suitable for laying ducks and meat egg dual-purpose type laying ducks.Egg is fed with this feedstuff additive premix compound
The Ovum Anas domestica that duck is produced carries out preserved egg yellow hard-core test.From 19 week old Longyan Boehmeria longispica Steud. ducks of 360 average weights, it is randomly divided into 2
Individual group, every group 6 repetitions, often repeat 30 ducks.Duck is raised in 40 × 40 × 60cm galvanized iron using Double-ladder formula single cage for examination
Silk duck cage.The laying ducks additive of feeding basal diet (matched group) and basal diet+300g/ ton alleviation preserved egg yellow hard-core is pre- respectively
Batch mixing (test group), terminates when feeding 4 months to test., without any other antioxidant, each treatment group is equal for test group daily ration
Using Semen Maydiss-Sorghum vulgare Pers.-miscellaneous meal type normal feedstuff, test feed is prepared with reference to Guangdong Academy of Agricultural Sciences's animal science institute
Formulate " laying ducks feeding standard " (draft) to carry out.During test, try chicken free choice feeding and drinking-water, other feedings and managements are according to routine
Feeding and management code is carried out.Every day entry laying ducks health status, feed intake and egg production.
Average daily gain: the weight of every duck searched for food feedstuff daily in experimental period.
Egg size: daily Total egg weight amount/number of laying eggs.
Daily output egg size: daily Total egg weight amount/laying duckses number.
Feedstuff-egg ratio: the ratio of daily ingestion amount and daily output egg size.
During test duck (35 week old), each repeats to choose 2 test ducks close to average weight, and wing venous are taken a blood sample 5ml, quiet
After putting 30min, 3500rpm is centrifuged 10min, separated plasma sample, and -80 DEG C preserve reduced glutathion in test plasma
(gsh), oxidized form of glutathione (gssg) content, antioxidative activities (t-aoc), total number born (t-sod) and paddy
Guang sweet peptide peroxidase (gsh-px) active biochemical index.
Examination Sanguis Anas domestica slurry gsh content adopts dithiobis formic acid (dtnb) colorimetry, and blood plasma t-aoc adopts fe2+With phenanthrene
Quinoline class forms firm complex colorimetry, and blood plasma total number born (t-sod) adopts xanthine oxidase, gluathione
Peptide peroxidase (gsh-px) activity adopts gsh exhaust method, and in blood plasma and egg yolk, mda content adopts thiobarbituricacidα-
(tba) colorimetry, in preserved egg yellow, content of protein carbonyl group adopts ultraviolet colorimetry, all in ultraviolet-visible spectrophotometer (north
Capital Rayleigh analytical tool company, uv-1601) on measured with the corresponding reagent box that Bioengineering Research Institute's offer is built up in Nanjing.
From experimental period the 20th, week old starts, the egg by monthly often repeating continuously to collect 3d is put into same as 1 batch
Labelling carried out by individual egg frame, continuous collection 6 batches, and the qualified egg after the Ovum Anas domestica sample of collection is checked and accepted through imported from Holland moba machine full inspection is adopted
With being pickled using plant ash+yellow mud salt pack.By 40kg Ovum Anas domestica proportioning straw ash 4.0kg, yellow mud 5kg, Sal 4.2kg,
Water 3.0kg, every for every batch group of 300 pieces of fresh duck eggs are uniformly wrapped up in one layer of stickiness mortar, are positioned over transparent plastic film closing
Warehouse, pickles 50d under room temperature.Take every group of every batch to pickle 100 pieces of the Salted duck egg of maturation, isolate complete preserved egg yellow tiling and put
It is placed in pallet, preserved egg yellow is toasted 15min in baking box (ks-410, China) in 145 DEG C, after room temperature cooling 15min, specialty
Preserved egg yellow is pulverized by assessment officer using gentle force, if having lump inside egg yolk it is determined that being hard-core, and separation lump is weighed, if
No lump, in comminution it is determined that being non-hard-core inside egg yolk.
Every group takes 24 Salted duck eggs pickling maturation, and the preserved egg yellow after every 4 pieces of separation Ovum Gallus domesticus album makes 1 sample, measures salted egg
Yellow malonaldehyde and content of protein carbonyl group.Shell thickness digimatic micrometer measures egg blunt end, middle part and sharp end and calculates meansigma methodss；
Eggshell strength, hangh unit and yolk color adopt intensity meter and full-automatic egg product analyser, and (orka, Beijing Tianxiang flies domain instrument
Equipment company limited) measure, measure and complete in 48h.
After taking the complete egg yolk sample liquid nitrogen flash freezer 5min pickling, natural separation goes out the egg yolk at center, cut into 0.5 ×
0.5cm size, keeps inspection surface to become initial condition, is fixed with 2.5% glutaraldehyde and be stored in 4 degree of refrigerator overnight；Take and fix
Sample repeatedly rinsed 3 times with the pbs buffer (ph 7.2) of 0.1m, every all over 10min；With 1% in ventilating kitchen under room temperature
Fix 30min after osmic acid, continue repeatedly to be rinsed 3 times with the pbs buffer (ph 7.2) of 0.1m, every time 10min.Rinse rear sample
Product graded ethanol (30%, 50%, 70%, 80%, 90% and 100%) is dehydrated step by step, and every kind of concentration is dehydrated 10min, finally
It is stored in drying tube with isoamyl acetate transition 15min dress sample；Scanning electron microscope (s-3700n Hitachi, day is adopted after sample metal spraying
Originally) microstructure to preserved egg yellow carries out photographic analysis.See Fig. 1 and Fig. 2.
Test data carries out significance using the one-way anova in sas (v8.1, sas institute, usa) and divides
Analysis, and dual comparison is carried out using bonferroni t-test.Result is represented with meansigma methodss ± standard error (means ± se), system
Meter significance level is p ＜ 0.05.
Table 1 Longyan Boehmeria longispica Steud. duck egg laying performance
| Process | Matched group | Test group |
| Laying rate (%) | 87.14±1.58 | 91.02±1.16 |
| Average egg weight (g) | 61.80±0.49 | 62.52±0.45 |
| Daily output egg size (g) | 53.89±1.01b | 56.92±0.84a |
| Feedstuff-egg ratio | 2.91±0.06b | 2.75±0.05a |
Note: colleague shoulder marking-up mother difference person represents significant difference (p < 0.05).
Result shows, the laying ducks additive premix that diet adds alleviation preserved egg yellow hard-core significantly improves peak period Longyan
Boehmeria longispica Steud. duck produces egg size daily, reduces feedstuff-egg ratio.Compared with matched group, daily output egg size improves 5.62%, feed-weight ratio and reduces
5.50%.It can be seen that, the laying ducks additive premix alleviating preserved egg yellow hard-core has the egg laying performance effect that is significantly improved.
Boehmeria longispica Steud. Sanguis Anas domestica slurry antioxygenic property in Longyan during table 2 test
| Project | Matched group | Test group |
| Reduced glutathion content (mg/l) | 9.37±0.57 | 11.98±0.59 |
| Oxidized form of glutathione content (μm ol/l) | 96.82±6.07 | 84.82±6.70 |
| Reduced glutathion/oxidized form of glutathione | 0.10±0.02 | 0.13±0.02 |
| Mda content (nmol/ml) | 12.58±0.97a | 8.98±0.95b |
| Antioxidative activities (u/ml) | 6.75±0.39b | 9.12±0.21a |
| Total number born activity (u/ml) | 54.59±3.18 | 59.66±2.15 |
| Activity of glutathione peroxidase (u/ml) | 2302.6±125.8 | 2322.1±100.06 |
Note: colleague shoulder marking-up mother difference person represents significant difference (p < 0.05).
Result shows, the laying ducks additive premix that diet adds alleviation preserved egg yellow hard-core significantly improves peak period Longyan
Boehmeria longispica Steud. Sanguis Anas domestica starches antioxidative activities, and reduces plasma malonaldehyde content, improves antioxidant ability of organism.
Longyan Boehmeria longispica Steud. Ovum Anas domestica quality during table 3 test
| Project | Matched group | Test group |
| Shell thickness (mm) | 0.334±0.01 | 0.323±0.01 |
| Eggshell strength (kgf) | 3.96±0.17 | 4.11±0.15 |
| Egg shape index (%) | 73.36±0.42 | 73.27±0.32 |
| Hangh unit | 70.93±0.62b | 76.51±0.73a |
| Yolk color | 12.13±0.10 | 12.00±0.11 |
| Eggshell weight (g) | 5.779±0.05 | 5.554±0.06 |
| Egg yolk ratio (%) | 29.38±0.39 | 29.31±0.24 |
| Egg yolk malonaldehyde (nmol/g) | 1.30±0.11a | 0.85±0.08b |
Note: colleague shoulder marking-up mother difference person represents significant difference (p < 0.05).
Result shows, the laying ducks additive premix that diet adds alleviation preserved egg yellow hard-core significantly improves test group Ovum Anas domestica
Hangh unit, and reduce Ovum Anas domestica yolk mda content, improve Ovum Anas domestica Egg Quality.
Table 4 preserved egg yellow hard-core, malonaldehyde, content of protein carbonyl group
| Project | Matched group | Test group |
| Hard-core rate (%) | 20.05±0.30a | 11.00±0.25b |
| Hard-core weight (g) | 0.98±0.09a | 0.75±0.06b |
| Malonaldehyde (nmol/g) | 172.59±7.29a | 129.81±5.05b |
| Protein carbonyl group (nmol/mg prot) | 6.13±0.40a | 4.57±0.28b |
Note: colleague shoulder marking-up mother difference person represents significant difference (p < 0.05).
Result shows, diet adds the test group preserved egg yellow hard-core of the laying ducks additive premix alleviating preserved egg yellow hard-core
Rate, hard-core weight, malonaldehyde and content of protein carbonyl group are substantially less than matched group.Illustrate to alleviate the feed additive of preserved egg yellow hard-core
Premix material can suppress preserved egg yellow fat and protein oxidation, reduces preserved egg yellow hard-core.
Learn from Fig. 1 and Fig. 2, matched group preserved egg yellow granule polyhedral structure is crude, and between gap, little oil droplet oozes out and substantially subtracts
Few, fuel-displaced less, boil rear hard-core preserved egg yellow and glue is resilient by one layer of wire-element parcel；And add in diet and alleviate salted egg
The laying ducks additive premix of yellow hard-core test group preserved egg yellow granule polyhedron arrangement in granulating arenaceous texture, between produce
Gap, oozes out much fatty little oil droplets, is attached to egg yolk granule surface between gap, boiling rear preserved egg yellow wire-element is in fragmentation
Shape.
In sum, feed the laying ducks of laying ducks additive premix and the matched group phase that this formula alleviates preserved egg yellow hard-core
Than, it is possible to increase laying ducks produce egg size daily, reduce feedstuff-egg ratio, effectively improve antioxidant ability of organism, improve Ovum Anas domestica quality, Ovum Anas domestica salts down
Preserved egg yellow fat and protein oxidation can substantially be suppressed during system, be conducive to infiltration and the dehydration of Sal, change egg yolk granule
Structure causes fuel-displaced, dusting, reduces preserved egg yellow hard-core.
Embodiment 2
Laying ducks are used for alleviating the laying ducks additive premix of preserved egg yellow hard-core, and wherein premix material includes following ingredients: containing big
Bean isoflavone 6mg/kg, vitamin e 60mg/kg, the carrier of described pre-mixing agent is stone powder, zeolite powder or bentonite.By this feedstuff
Additive pre-mixing agent is added in laying ducks complete feed according to the ratio of 200～300g/ ton.
Embodiment 3
Laying ducks are used for alleviating the laying ducks additive premix of preserved egg yellow hard-core, and wherein premix material includes following ingredients: containing big
Bean isoflavone 15mg/kg, vitamin e 20mg/kg, the carrier of described pre-mixing agent is stone powder, zeolite powder or bentonite.Originally raised
Feed additives pre-mixing agent is added in laying ducks complete feed according to the ratio of 200～300g/ ton.
Embodiment 4
Laying ducks are used for alleviating the laying ducks additive premix of preserved egg yellow hard-core, and wherein premix material includes following ingredients: containing big
Bean isoflavone 10mg/kg, vitamin e 100mg/kg, the carrier of described pre-mixing agent is stone powder, zeolite powder or bentonite.Originally raised
Feed additives pre-mixing agent is added in laying ducks complete feed according to the ratio of 200～300g/ ton.
Product mixed by above-described embodiment 2,3,4, does comparing of identical test process with embodiment 1, all
The result of the test approximate with embodiment 1 can be obtained, here does not repeat one by one.In each embodiment, each component raw material is all commercially available
Obtain.
Above example is used merely to explain the present invention, and protection scope of the present invention is not intended to be limited to above enforcement
Example.The those of ordinary skill of described technical field, all can be real according to above present disclosure and the taken scope of each parameter
The existing purpose of the present invention.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not subject to above-described embodiment
Limit, other any spirit without departing from the present invention and the change made under principle, modification, replacement, combine, simplify,
All should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (7)
1. a kind of alleviate preserved egg yellow hard-core laying ducks additive premix it is characterised in that: this premix material is by additive and carrier
Composition, described additive component is: soybean isoflavone and vitamin e.
2. according to claim 1 alleviate preserved egg yellow hard-core laying ducks additive premix it is characterised in that: described adds
Plus the addition of agent is soybean isoflavone 6～15mg/kg, vitamin e 20～100mg/kg.
3. according to claim 1 and 2 alleviate preserved egg yellow hard-core laying ducks additive premix it is characterised in that: described
Carrier be maize cob meal, zeolite powder or bentonite.
4. the laying ducks additive premix of the alleviation preserved egg yellow hard-core described in any one of claims 1 to 3 is used for laying ducks in preparation
With the application in the mixed feed of meat egg dual-purpose type duck.
5. application according to claim 4 it is characterised in that: the laying ducks additive premix of described alleviation preserved egg yellow hard-core
Material adds to 4% premix material according to the ratio of 5～7.5kg/ ton.
6. application according to claim 4 it is characterised in that: the laying ducks additive premix of described alleviation preserved egg yellow hard-core
Material adds to 20% concentrated feed according to the ratio of 1～1.5kg/ ton.
7. application according to claim 4 it is characterised in that: the laying ducks additive premix of described alleviation preserved egg yellow hard-core
Material adds to perfect compound feed according to the ratio of 200～300g/ ton.
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| CN201610692562.6A CN106360036A (en) | 2016-08-19 | 2016-08-19 | Laying duck additive premix for relieving salted egg yolk core hardening and use thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110367381A (en) * | 2019-08-09 | 2019-10-25 | 广东省农业科学院动物科学研究所 | A kind of laying duck drinking water additive, laying duck drinking-water formula and its preparation method and application |
| CN111838449A (en) * | 2020-06-10 | 2020-10-30 | 广东省农业科学院动物科学研究所 | Feed premix for improving egg quality and application thereof |
| CN116616373A (en) * | 2023-06-01 | 2023-08-22 | 广东省农业科学院动物科学研究所 | Application of beef tallow in improving hard heart rate of salted duck eggs |
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| CN106071309A (en) * | 2016-06-20 | 2016-11-09 | 安徽省家牧动物营养科技有限公司 | A kind of feed additive for egg-laying ducks that can significantly improve egg yolk color depth |
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- 2016-08-19 CN CN201610692562.6A patent/CN106360036A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106071309A (en) * | 2016-06-20 | 2016-11-09 | 安徽省家牧动物营养科技有限公司 | A kind of feed additive for egg-laying ducks that can significantly improve egg yolk color depth |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110367381A (en) * | 2019-08-09 | 2019-10-25 | 广东省农业科学院动物科学研究所 | A kind of laying duck drinking water additive, laying duck drinking-water formula and its preparation method and application |
| CN111838449A (en) * | 2020-06-10 | 2020-10-30 | 广东省农业科学院动物科学研究所 | Feed premix for improving egg quality and application thereof |
| CN116616373A (en) * | 2023-06-01 | 2023-08-22 | 广东省农业科学院动物科学研究所 | Application of beef tallow in improving hard heart rate of salted duck eggs |
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Application publication date: 20170201 |