CN106359411A - Compound bactericide for preventing and controlling soft rot of kiwifruits and use method of compound bactericide - Google Patents

Compound bactericide for preventing and controlling soft rot of kiwifruits and use method of compound bactericide Download PDF

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Publication number
CN106359411A
CN106359411A CN201610779445.3A CN201610779445A CN106359411A CN 106359411 A CN106359411 A CN 106359411A CN 201610779445 A CN201610779445 A CN 201610779445A CN 106359411 A CN106359411 A CN 106359411A
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China
Prior art keywords
parts
fructus actinidiae
soft rot
actinidiae chinensiss
prevention
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Pending
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CN201610779445.3A
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Chinese (zh)
Inventor
徐朝云
黎晓茜
龙友华
尹显慧
吴小毛
张承
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Guizhou Nongda Qiansheng Ecological Agriculture Co Ltd
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Guizhou Nongda Qiansheng Ecological Agriculture Co Ltd
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Priority to CN201610779445.3A priority Critical patent/CN106359411A/en
Publication of CN106359411A publication Critical patent/CN106359411A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a compound bactericide for preventing and controlling soft rot of kiwifruits and a use method of the compound bactericide. The compound bactericide is a suspoemulsion prepared from the following components in parts by weight: 3 parts of fluxapyroxad, 20 parts of tetramycin, 3 parts of sodium-rich calcium, 15 parts of additive and 20 parts of filler. The fluxapyroxad and the tetramycin in the compound bactericide disclosed by the invention are compounded to realize a remarkable synergic effect; the compound agent is safe and harmless to the growth of the kiwifruits, has the advantages of good environmental compatibility, simple operation, low production cost, good prevention and control effect and the like, and is easy to popularize; fruit diseases of the kiwifruits can be effectively prevented and controlled, the fruiting rate of the fruits is improved, and the quality and storage properties of the fruits are improved.

Description

A kind of composite bactericide of prevention and control Fructus actinidiae chinensiss soft rot and its using method
Technical field
The present invention relates to a kind of composite bactericide of prevention and control Fructus actinidiae chinensiss soft rot and its using method, belong to agricultural economy fruit Woods techniques of pest control field.
Background technology
Fructus actinidiae chinensiss (actinidia) are described as " king of fruit ", not only sweet mouthfeel, delicious flavour, are also rich in abundant Vitamin c, potassium, cellulose etc., it is subject to wide because its fruit contains higher nutritive value, unique flesh color and local flavor The favor of big consumer, has become a kind of emerging fruit tree in the whole world at present.However, easily there is decayed fruit phenomenon in Fructus actinidiae chinensiss in recent years, The orchard deuterogenesis decayed fruit that bears fruit came off before this, after be that the aggravation of storage period fruit is rotted, decayed fruit problem has become restriction macaque Fructus actinidiae chinensiss are produced and constitute a serious threat by the key factor that Fructus Persicae produces.
It is reported that, Fructus actinidiae chinensiss decayed fruit is mainly by botrytis cinerea (botrytis cinerea), Fructus Vitis viniferae seat chamber bacterium (botryosphaeria dothidea), Fu Shi Fructus Vitis viniferae spore cup fungi (botryotiniafuckeliana), pestalotia bacteria (pestalotiopsis sp.), fusarium prolifertum (fusariumproliferatum), Phomopsis bacterium (phomopsis Sp.) cause with 7 kinds of soft rot pathogenic bacterium such as alternaric bacteria (aiternaria sp.).Xiuwen County Fructus actinidiae chinensiss soft rot is carried out Isolation identification draws and causes Fructus actinidiae chinensiss soft rot nosophyte to have Fructus Vitis viniferae seat chamber bacterium (botryosphaeria dothidea), disk many Hair spore bacterium (pestalotiopsis sp.), fusarium prolifertum (fusariumproliferatum), Phomopsis bacterium (phomopsis sp.).
Fructus actinidiae chinensiss soft rot is short for incubation period, and infection velocity is fast, fruit can be caused to rot on a large scale in several days, currently for Fructus actinidiae chinensiss Disease-Control for Soft-Rotting report is less, and agriculturally mainly based on chemical prevention, and the preventing and treating of Fructus actinidiae chinensiss soft rot there is no spy Effect medicament, single dose prevention effect is also undesirable.
Content of the invention
It is an object of the invention to provide a kind of composite bactericide of prevention and control Fructus actinidiae chinensiss soft rot and its using method, this Bright filter out the preferable two kinds of antibacterial of prevention effect for its main pathogenic fungi Fructus Vitis viniferae seat chamber bacterium, i.e. fluxapyroxad and four mould Element, carries out Disease-Control for Soft-Rotting using both antibacterial fluxapyroxad, tetramycin 3:20 by weight ratio, reaches significantly Prevention effect.
In order to solve above-mentioned technical problem, the present invention adopts the following technical scheme that: a kind of prevention and control Fructus actinidiae chinensiss soft rot Composite bactericide, calculates in parts by weight, and it is by 2～4 parts of fluxapyroxad, 15～25 parts of tetramycin, amino acid calcium 2～4 Part, 10～20 parts of auxiliary agent and 10～30 parts of suspension emulsions made of filler.
Further, calculate in parts by weight, it is by 3 parts of fluxapyroxad, 20 parts of tetramycin, 3 parts of amino acid calcium, auxiliary agent 15 parts and 20 parts of wettable powders made of filler.
Described auxiliary agent is one of dispersant, wetting agent, suspending agent and stabilizer or two or more group Close.
Described filler is one of clay, Pulvis Talci, kieselguhr, meerschaum, Kaolin, white carbon and montmorillonite Or two or more combination.
Meanwhile, the present invention also provides a kind of using method of the composite bactericide for above-mentioned prevention and control Fructus actinidiae chinensiss soft rot, The method is to carry out spraying for the first time by the bud stage in Fructus actinidiae chinensiss after 2000 times of compound sterilizing dilution agent, thanks 15～20 days after spending Sprayed for the second time, harvesting carries out third time the previous moon and sprays, thus reaching significant Disease-Control for Soft-Rotting effect.
Compared with prior art, it is an advantage of the current invention that: the present invention is to according to fluxapyroxad, two kinds of medicines of tetramycin Features reasonably compounded, maximize favourable factors and minimize unfavourable ones, quick-acting is good, wide sterilization spectrum, the present invention has to Fructus actinidiae chinensiss soft rot Significantly prevention effect, safe and harmless to Kiwi growth, have that Environmental compatibility is good, simple to operate, low production cost, prevention and control The advantages of effect is good, it is easy to promote, can promote the increasing both production and income of Fructus actinidiae chinensiss industry, improve fruit quality, and it is huge to be that orchard worker brings Big economic benefit.
Specific embodiment
In order that the object, technical solutions and advantages of the present invention are clearer, with reference to embodiment the present invention is made into One step describes in detail.
Toxicity Determination is the important step of agriculture chemical compounding agent, can be obtained in a large number for reference by Toxicity Determination Data.Agriculture chemical compounding can have potentiation and antagonism, if antagonism, as unqualified.Therefore, first below Toxicity Determination is carried out to the antibacterial providing, filters out fluxapyroxad, the preferable antibacterial of two kinds of effects of tetramycin, more right Fluxapyroxad, tetramycin carry out different ratio co-toxicity test:
Test material is Fructus actinidiae chinensiss Bacteria erwinia Fructus Vitis viniferae seat chamber bacterium.Test pesticide by BASF China, Liaoning Wkioc Bioengineering Co., Ltd. provides.Weigh 3 parts of fluxapyroxad, 20 parts of tetramycin, 3 parts of amino acid calcium, auxiliary agent 15 Part and 20 parts of filler, are separately added into a certain amount of aquesterilisa and are made into mother solution, be diluted to a series of concentration with mother solution, each process 4 repetitions of setting.
Test adopts mycelial growth rate algoscopy.According to 6 mass concentration gradients of the every kind of medicament configuration of trial test result, Every kind of reagent agent is added in the pda plating medium be cooled to 45 DEG C about and is respectively prepared flat board, inoculate single diameter 6mm Bacteria cake of the same age, if aquesterilisa is comparison, each process sets 4 repetitions.Check each after culture 3d in 27 DEG C of constant incubators Process the growing state of bacterium, rice word interior extrapolation method measures colony diameter, calculate bacteriostasis rate.
Bacteriostasis rate (%)=(comparison colony diameter-process colony diameter)/(comparison colony diameter-bacteria cake diameter) × 100.
Growth bacteriostasis rate is converted into suppression ratio and as vertical coordinate (y), pharmacy quality concentration is converted to 10 as bottom Logarithm value as abscissa (x), according to the linear regression relation between bacteriostasis rate and log concentration try to achieve virulence regression equation and Correlation coefficient, and calculate ec50.
Using the abundant method of Sun Yun, calculate co-toxicity coefficient ctc, pass judgment on the co-toxicity effect of two kinds of medicaments with ctc value.Ctc value It is antagonism less than 80, is potentiation more than 120, be summation action between 80-120.
The single dose toxicity index ti=(ec of standard agent50The ec of/reagent agent50)×100
The actual toxicity index ati=(ec of standard agent50The ec of/Mixed Pharmacy50) × 100 theoretical toxicity index tti= A medicament toxicity index × a medicament percentage+b medicament toxicity index × b medicament institute in mixing assembles in mixing assembles The percentage ratio accounting for
Co-toxicity coefficient ctc=ati/tti × 100 experiment mixture result is as follows:
The table 1 fluxapyroxad and tetramycin different ratio toxicity test to Fructus actinidiae chinensiss soft rot
As shown in Table 1, fluxapyroxad and two kinds of composite reagents of tetramycin, have higher potentiation to make Fructus actinidiae chinensiss soft rot With its fluxapyroxad connects tetramycin proportioning 3:20 potentiation synergistic effect by weight most preferably, remaining 5:20,6:20,7:20 Deng all showing different degrees of potentiation, co-toxicity coefficient is all higher than 120.
Embodiment 1:
A kind of composite bactericide of prevention and control Fructus actinidiae chinensiss soft rot of the present invention, calculates in parts by weight, and it is by fluorine azoles bacterium Amide hangs 3 parts, 20 parts of tetramycin, 3 parts of amino acid calcium, 15 parts of auxiliary agent and 20 parts of wettable powders made of filler, then will 2000 times of this wettable powder dilution agent is stand-by.Experimental field it is selected in Xiuwen County gold woods Fructus actinidiae chinensiss Experimental Base, test material is selected expensive Long Kiwifruit Cultivars.Apply according to scheme, five process, each processes 3 repetitions, 15 cells, divides 15 hillslope processes, Every 10 plants of Fructus actinidiae chinensiss of cell, totally 150 plants of Fructus actinidiae chinensiss fruit trees, ck: blank；Process a: spray fluxapyroxad suspending agent 2000 Times；Process b: spray 1000 times of tetramycin water preparation；Process c: spray 2000 times of liquid of 5:20 composite bactericide；Process d: spray 3:20 2000 times of liquid of composite bactericide.Respectively bud stage, thank spend after sprayed and harvested within 15～20 days the spray of the previous moon for the second time Apply.
Carry out fruit-setting rate investigation, each processes and randomly draws 5 plants of Fructus actinidiae chinensiss and carry out fruit disease after fruit maturation after bearing fruit, Carry out the mensure of storge quality, fruit quality after fruit harvesting.
With x, disease scale standard (represents that scab adds up diameter):
0 grade: do not fall ill；1 grade: 0 ＜ x≤1cm；2 grades: 1 ＜ x≤2cm；3 grades: 2 ＜ x≤3cm；4 grades: 3 ＜ x≤4cm；5 Level: 4 ＜ x≤5cm；6 grades: 5cm≤x
Sickness rate (%)=(morbidity fruit number/investigation fruit number) × 100
Fruit-setting rate (%)=(number is spent in fruit number/pollination) × 100
Disease index=∑ (disease fruit numbers at different levels × representative values at different levels)/(investigating total fruit number × highest level representative value) × 100
Preventive effect (%)=(comparison disease index-process disease index)/comparison disease index × 100
Mushy fruit rate (%)=softening fruit number/investigation sum × 100
Table 2 kiwifruit fruit disease a situation arises investigation
Kiwifruit fruit quality and storage performance after table 3 harvesting
By table 2 and table 3 it can be seen that compound drug d is substantially better than single dose to Fructus actinidiae chinensiss Disease-Control for Soft-Rotting effect, preventing and treating Effect reaches 89.20%, and compared with comparison ck, fruit-setting rate increases by 24.93%.After this compound drug is processed, kiwifruit fruit is hard Degree, Soluble adhesion molecule and Determination of Vitamin C are processed apparently higher than other, and difference is extremely aobvious compared with comparison ck, single dose process Write；Dispenser processes storage performance and is substantially better than matched group, and storage performance is good, and the longest storage natural law was up to 82 days.

Claims (5)

1. a kind of composite bactericide of prevention and control Fructus actinidiae chinensiss soft rot it is characterised in that: calculate in parts by weight, it is by fluorine azoles bacterium It is outstanding that 2～4 parts of amide, 15～25 parts of tetramycin, 2～4 parts of amino acid calcium, 10～20 parts of auxiliary agent and 10～30 parts of filler are made Emulsion.
2. prevention and control Fructus actinidiae chinensiss soft rot according to claim 1 composite bactericide it is characterised in that: in parts by weight Calculate, it be by 3 parts of fluxapyroxad, 20 parts of tetramycin, 3 parts of amino acid calcium, 15 parts of auxiliary agent and 20 parts of filler make wettable Property powder.
3. prevention and control Fructus actinidiae chinensiss soft rot according to claim 1 and 2 composite bactericide it is characterised in that: described auxiliary agent For one of dispersant, wetting agent, suspending agent and stabilizer or two or more combination.
4. prevention and control Fructus actinidiae chinensiss soft rot according to claim 1 and 2 composite bactericide it is characterised in that: described fills out Expect for one of clay, Pulvis Talci, kieselguhr, meerschaum, Kaolin, white carbon and montmorillonite or two or more Combination.
5. the using method of the composite bactericide of prevention and control Fructus actinidiae chinensiss soft rot as described in a kind of any one as Claims 1 to 4, It is characterized in that: the bud stage in Fructus actinidiae chinensiss after 2000 times of compound sterilizing dilution agent is carried out spraying for the first time, thank 15 after spending～ Sprayed for the second time within 20 days, harvesting carries out third time the previous moon and sprays.
CN201610779445.3A 2016-08-31 2016-08-31 Compound bactericide for preventing and controlling soft rot of kiwifruits and use method of compound bactericide Pending CN106359411A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107793217A (en) * 2017-10-24 2018-03-13 贵州大学 A kind of red kiwi fruit Pesticidal foliage fertilizer and preparation method thereof
CN107926405A (en) * 2017-11-16 2018-04-20 贵阳学院 A kind of Synthetical prevention method of Kiwi berry soft rot
CN108935499A (en) * 2018-08-20 2018-12-07 贵州道元生物技术有限公司 A kind of combination sterilization composition for preventing and treating Kiwi berry soft rot
CN112674107A (en) * 2021-01-11 2021-04-20 河北省农林科学院植物保护研究所 Bactericidal composition for preventing and treating fruit and vegetable diseases and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103385256A (en) * 2012-05-07 2013-11-13 陕西韦尔奇作物保护有限公司 Synergistic sterilization composition containing Xemium and triazoles
WO2015121231A1 (en) * 2014-02-13 2015-08-20 Bayer Cropscience Ag Active compound combinations comprising phenylamidine compounds and further fungicides
CN104982445A (en) * 2015-07-25 2015-10-21 海利尔药业集团股份有限公司 Bactericidal composition comprising fluxapyroxad and tetramycin

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103385256A (en) * 2012-05-07 2013-11-13 陕西韦尔奇作物保护有限公司 Synergistic sterilization composition containing Xemium and triazoles
WO2015121231A1 (en) * 2014-02-13 2015-08-20 Bayer Cropscience Ag Active compound combinations comprising phenylamidine compounds and further fungicides
CN104982445A (en) * 2015-07-25 2015-10-21 海利尔药业集团股份有限公司 Bactericidal composition comprising fluxapyroxad and tetramycin

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107793217A (en) * 2017-10-24 2018-03-13 贵州大学 A kind of red kiwi fruit Pesticidal foliage fertilizer and preparation method thereof
CN107926405A (en) * 2017-11-16 2018-04-20 贵阳学院 A kind of Synthetical prevention method of Kiwi berry soft rot
CN108935499A (en) * 2018-08-20 2018-12-07 贵州道元生物技术有限公司 A kind of combination sterilization composition for preventing and treating Kiwi berry soft rot
CN112674107A (en) * 2021-01-11 2021-04-20 河北省农林科学院植物保护研究所 Bactericidal composition for preventing and treating fruit and vegetable diseases and application thereof

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Application publication date: 20170201