CN107173393A - Postharvest disease and somatotrophic microbial inoculum, preparation method and application before preventing and treating strawberry is adopted - Google Patents
Postharvest disease and somatotrophic microbial inoculum, preparation method and application before preventing and treating strawberry is adopted Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
Postharvest disease and the microbial inoculum of promotion growth before strawberry is adopted are prevented and treated the invention discloses a kind of, raw material is made up of the component of following mass percent：85~95% mixed fermentation bacterium solution, 0.05~0.1% Tween 80,0.2~0.5% calcium chloride, 0.1~0.5% sodium metasilicate, 0.75~2% chitosan, surplus is water；Mixed fermentation bacterium solution is by the strange zymocyte liquids of yeast Y 3 of plum, the zymocyte liquids of Burkholderia B 1 and the zymocyte liquids of enterobacteria B 61 according to 1：1~2：0.5~1 mass ratio composition.In addition, present invention also offers a kind of preparation method and applications for preventing and treating postharvest disease and the microbial inoculum for promoting growth before strawberry is adopted.The microbial inoculum preharvest spraying of the present invention, effectively reduces the incidence of postharvest disease of strawberry, reduction strawberry fruit adopt after rotting rate, further promote strawberry growth, be the green production of strawberry and fresh-keeping provide new way.
Description
Technical field
The invention belongs to microbial technology field, and in particular to postharvest disease and promotion grow before a kind of preventing and treating strawberry is adopted
Microbial inoculum, preparation method and applications.
Background technology
Fragaria rose family Fragaria, fruit color is bright-coloured, pulp sour and sweet palatability, with very high nutrition and economic valency
Value.But it is due to that its pericarp is tender and lovely, easily produces machinery wound, is easily caused post-harvest physiology imbalance, moisture loss and pathogen infection.
Strawberry causes the pathogen of fruit rot to derive from field mostly, therefore, taken before field in postharvest storage or transporting procedures
Effective measures reduce the content of molds on post-harvest fruits surface, are played an important role for reducing postharvest decay.
The main method of postharvest decay is to use chemical bactericide before reduction strawberry is adopted at present, and chemical bactericide can give ring
The problems such as border and human health threaten and brought the resistance to the action of a drug of pathogen, so that it is increasingly limited using scope
System, so it is imperative to find other prevention and controls.
The content of the invention
Postharvest disease and the microbial inoculum of promotion growth, solve strawberry and adopt preceding appearance before a kind of preventing and treating strawberry that the present invention is provided is adopted
Easily occurs disease, the problem of easily being rotted after adopting.
First purpose of the present invention is to provide postharvest disease and the microbial inoculum of promotion growth before a kind of preventing and treating strawberry is adopted, and it is former
Material is made up of the component of following mass percent：85~95% mixed fermentation bacterium solution, 0.05~0.1% Tween 80,0.2
~0.5% calcium chloride, 0.1~0.5% sodium metasilicate, 0.75~2% chitosan, surplus are supplied with water to 100%；
Total plate count is 1.0 × 10 in the mixed fermentation bacterium solution6~1.0 × 1011cfu/ml；
The mixed fermentation bacterium solution is by Metschnikowia zizyphicola Y-3 zymocyte liquids, Burkholderia B-1
(Burkholderia contaminans B-1) zymocyte liquid and enterobacteria B-6-1 (Enterobacter cowanii
B-6-1) zymocyte liquid is according to 1：1~2：0.5~1 mass ratio is mixed；
Wherein, the Metschnikowia zizyphicola Y-3 zymocyte liquids are by Metschnikowia
The pure strain culturings of zizyphicola Y-3 are obtained to late log phase；The Burkholderia B-1 zymocyte liquids are by Burkholderia B-
What 1 pure strain culturing was obtained to late log phase；The enterobacteria B-6-1 zymocyte liquids are by the pure strain culturings of enterobacteria B-6-1
Obtained to late log phase.
It is preferred that, postharvest disease and the microbial inoculum of promotion growth before the preventing and treating strawberry is adopted, its raw material is by following quality percentage
The component composition of ratio：90% mixed fermentation bacterium solution, 0.08% Tween 80,0.3% calcium chloride, 0.3% sodium metasilicate,
1% chitosan, surplus is supplied with water to 100%；
Total plate count is 1.0 × 10 in the mixed fermentation bacterium solution8cfu/ml；
The mixed fermentation bacterium solution is by Metschnikowia zizyphicola Y-3 zymocyte liquids, Burkholderia B-1
Zymocyte liquid and enterobacteria B-6-1 zymocyte liquids are according to 2：2：1 mass ratio is mixed.
Second object of the present invention is to provide a kind of system for preventing and treating postharvest disease and the microbial inoculum for promoting growth before strawberry is adopted
Preparation Method, comprises the following steps：
Step 1, by Metschnikowia zizyphicola Y-3 pure bacterial strain -80 DEG C take out after at 28 DEG C
48h is cultivated in PDA plate line, and then picking single bacterium is fallen within NYDB fluid nutrient mediums, with 200r/min hunting speed culture
72h, obtains Metschnikowia zizyphicola Y-3 Preliminary fermentation bacterium solution；
By Burkholderia B-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, choose
Take single bacterium to fall within YSP fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain the first of Burkholderia B-1 and originate
Yeast-like fungi liquid；
By enterobacteria B-6-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, choose
Take single bacterium to fall within LB fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain the first of enterobacteria B-6-1 and originate
Yeast-like fungi liquid；
Step 2, Metschnikowia zizyphicola Y-3 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums
In, then at 28 DEG C with 200r/min hunting speed cultures to late log phase, obtain Metschnikowia
Zizyphicola Y-3 zymocyte liquids；
Burkholderia B-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain Burkholderia B-1 zymocyte liquids to late log phase；
Enterobacteria B-6-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain enterobacteria B-6-1 zymocyte liquids to late log phase；
Step 3, by the Metschnikowia zizyphicola Y-3 zymocyte liquids, the Burkholderia B- that are obtained in step 2
1 zymocyte liquid and enterobacteria B-6-1 zymocyte liquids press 1：1~2：0.5~1 mass ratio mixing, obtains mixed fermentation bacterium solution；
Step 4, weigh mass percent be 90% above-mentioned mixed fermentation bacterium solution, 0.05%~0.1% Tween 80,
0.2~0.5% calcium chloride, 0.1~0.5% sodium metasilicate, 0.75%~2% chitosan, surplus supply 100% with water；
Step 5, Tween 80, calcium chloride, the silicon weighed in step 4 is added in the mixed fermentation bacterium solution weighed into step 4
Sour sodium, chitosan and water, after being well mixed, that is, obtain postharvest disease and the microbial inoculum of promotion growth before the preventing and treating strawberry is adopted.
It is preferred that, following components are included in every liter of YSP fluid nutrient medium：Peptone 10g, yeast extract 5g, glucose
10g, surplus is water.
It is preferred that, following components are included in every liter of NYDB fluid nutrient medium：Beef extract 8g, yeast extract 5g, grape
Sugared 10g, surplus is water.
It is preferred that, following components are included in every liter of LB fluid nutrient medium：Tryptone 10g, yeast extract 5g,
NaCl 10g, surplus is water.
Third object of the present invention is to provide a kind of preventing and treating strawberry and adopts preceding postharvest disease and promote the microbial inoculum of growth in grass
Application before the certain kind of berries grows and adopted in postharvest disease preventing and treating.
Microorganism Deposit Information explanation：
Metschnikowia zizyphicola Y-3：It has been preserved in China typical culture collection center, address：In
Wuhan University of Wuhan City of state, its preserving number is CCTCC NO.M2016564, preservation date：On October 17th, 2016.
Burkholderia B-1 (Burkholderia contaminans B-1)：It has been preserved in China typical culture collection
Center, address：Wuhan University of Wuhan, China city, preserving number is CCTCC NO.M2014076, preservation date：On March 7th, 2014.
Enterobacteria B-6-1 (Enterobacter cowanii B-6-1)：It has been preserved in China typical culture collection
The heart, address：Wuhan University of Wuhan, China city, preserving number is CCTCC NO.M2012398, preservation date：On October 11st, 2012.
Wherein, the Authorization Notice No. that Burkholderia B-1 was authorized on October 12nd, 2016 is the B of CN 104109645
Chinese patent《For the anti-microbial inoculum of bacterial strain and biological and ecological methods to prevent plant disease, pests, and erosion for preventing and treating postharvest disease of fruits and vegetables》In be disclosed；
The China that the Authorization Notice No. that enterobacteria B-6-1 was authorized on April 27th, 2016 is the B of CN 103952327
Patent《Prevent and treat biological antagonist bacterial strain, its preparation method and its application of postharvest disease of fruits and vegetables》In be disclosed.
Compared with prior art, the beneficial effects of the present invention are：
1) postharvest disease and the microbial inoculum of promotion growth can be effective in preharvest spraying before the preventing and treating strawberry that the present invention is provided is adopted
The incidence of postharvest disease of strawberry is reduced, while strawberry can also be promoted to grow.
2) postharvest disease and the microbial inoculum of promotion growth are sprayed after adopting and can effectively dropped before the preventing and treating strawberry that the present invention is provided is adopted
Low fruit surface content of molds, reduction strawberry fruit adopt after rotting rate, be strawberry green production and it is fresh-keeping provide new way.
Brief description of the drawings
Fig. 1 is that the preventing and treating strawberry that preharvest spraying embodiment 2 is prepared adopts preceding postharvest disease and promotes the microbial inoculum of growth to grass
The influence result figure of certain kind of berries fruit gray mold；
Fig. 2 is that the preventing and treating strawberry that preharvest spraying embodiment 2 is prepared adopts preceding postharvest disease and promotes the microbial inoculum of growth to grass
The influence result figure that certain kind of berries fruit rots naturally.
Embodiment
In order that those skilled in the art more fully understand that technical scheme can be practiced, with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Experimental method described in various embodiments of the present invention, is conventional method unless otherwise specified.
Embodiment 1
It is a kind of prevent and treat strawberry adopt before postharvest disease and promote growth microbial inoculum, its raw material by following mass percent component
Composition：85% mixed fermentation bacterium solution, 0.05% Tween 80,0.5% calcium chloride, 0.1% sodium metasilicate, 2% shell gather
Sugar, surplus is supplied with water to 100%；
Total plate count is 1.0 × 10 in mixed fermentation bacterium solution6cfu/ml；
Mixed fermentation bacterium solution is by Metschnikowia zizyphicola Y-3 zymocyte liquids, Burkholderia B-1 zymophytes
Liquid and enterobacteria B-6-1 zymocyte liquids composition are according to 1：1.5：0.8 mass ratio is mixed；
Wherein, Metschnikowia zizyphicola Y-3 zymocyte liquids are by Metschnikowia
The pure strain culturings of zizyphicola Y-3 are obtained to late log phase；Burkholderia B-1 zymocyte liquids are pure by Burkholderia B-1
Strain culturing is obtained to late log phase；Enterobacteria B-6-1 zymocyte liquids are by the pure strain culturings of enterobacteria B-6-1 to logarithm
What latter stage obtained.
Specific implementation step is as follows：
Step 1, by Metschnikowia zizyphicola Y-3 pure bacterial strain -80 DEG C take out after at 28 DEG C
48h is cultivated in PDA plate line, and then picking single bacterium is fallen within NYDB fluid nutrient mediums, with 200r/min hunting speed culture
72h, obtains Metschnikowia zizyphicola Y-3 Preliminary fermentation bacterium solution；
By Burkholderia B-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, choose
Take single bacterium to fall within YSP fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain the first of Burkholderia B-1 and originate
Yeast-like fungi liquid；
By enterobacteria B-6-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, choose
Take single bacterium to fall within LB fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain the first of enterobacteria B-6-1 and originate
Yeast-like fungi liquid；
Wherein, following components are included in every liter of YSP fluid nutrient medium：Peptone 10g, yeast extract 5g, glucose 10g, it is remaining
Measure as water；
Wherein, following components are included in every liter of NYDB fluid nutrient medium：Beef extract 8g, yeast extract 5g, glucose 10g,
Surplus is water；
Wherein, following components are included in every liter of LB fluid nutrient medium：Tryptone 10g, yeast extract 5g, NaCl
10g, surplus is water；
Step 2, Metschnikowia zizyphicola Y-3 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums
In, then at 28 DEG C with 200r/min hunting speed cultures to late log phase, obtain Metschnikowia
Zizyphicola Y-3 zymocyte liquids；
Burkholderia B-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain Burkholderia B-1 zymocyte liquids to late log phase；
Enterobacteria B-6-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain enterobacteria B-6-1 zymocyte liquids to late log phase；
Step 3, by the Metschnikowia zizyphicola Y-3 zymocyte liquids, the Burkholderia B- that are obtained in step 2
1 zymocyte liquid and enterobacteria B-6-1 zymocyte liquids are according to 1：1.5：0.8 mass ratio mixing, obtains mixed fermentation bacterium solution；
Step 4, above-mentioned mixed fermentation bacterium solution, 0.05% Tween 80,0.5% that mass percent is 85% are weighed
Calcium chloride, 0.1% sodium metasilicate, 2% chitosan, surplus supply 100% with water；
Step 5, Tween 80, calcium chloride, the silicon weighed in step 4 is added in the mixed fermentation bacterium solution weighed into step 4
Sour sodium, chitosan and water, after being well mixed, that is, obtain postharvest disease and the microbial inoculum of promotion growth before the preventing and treating strawberry is adopted.
Embodiment 2
It is a kind of prevent and treat strawberry adopt before postharvest disease and promote growth microbial inoculum, its raw material by following mass percent component
Composition：90% mixed fermentation bacterium solution, 0.08% Tween 80,0.3% calcium chloride, 0.3% sodium metasilicate, 1% shell gather
Sugar, surplus is supplied with water to 100%；
Total plate count is 1.0 × 10 in mixed fermentation bacterium solution8cfu/ml；
Mixed fermentation bacterium solution is by Metschnikowia zizyphicola Y-3 zymocyte liquids, Burkholderia B-1 zymophytes
Liquid and enterobacteria B-6-1 zymophytes are according to 1：1：0.5 mass ratio is mixed.
Specific implementation step is as follows：
Step 1, by Metschnikowia zizyphicola Y-3 pure bacterial strain~80 DEG C take out after at 28 DEG C
48h is cultivated in PDA plate line, and then picking single bacterium is fallen within NYDB fluid nutrient mediums, with 200r/min hunting speed culture
72h, obtains Metschnikowia zizyphicola Y-3 Preliminary fermentation bacterium solution；
By Burkholderia B-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, choose
Take single bacterium to fall within YSP fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain the first of Burkholderia B-1 and originate
Yeast-like fungi liquid；
By enterobacteria B-6-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, choose
Take single bacterium to fall within LB fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain the first of enterobacteria B-6-1 and originate
Yeast-like fungi liquid；
Wherein, NYDB fluid nutrient mediums, YSP fluid nutrient mediums and LB fluid nutrient mediums be the same as Example 1；
Step 2, Metschnikowia zizyphicola Y-3 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums
In, then at 28 DEG C with 200r/min hunting speed cultures to late log phase, obtain Metschnikowia
Zizyphicola Y-3 zymocyte liquids；
Burkholderia B-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain Burkholderia B-1 zymocyte liquids to late log phase；
Enterobacteria B-6-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain enterobacteria B-6-1 zymocyte liquids to late log phase；
Step 3, by the Metschnikowia zizyphicola Y-3 zymocyte liquids, the Burkholderia B- that are obtained in step 2
1 zymocyte liquid and enterobacteria B-6-1 zymocyte liquids are according to 1：1：0.5 mass ratio mixing, obtains mixed fermentation bacterium solution；
Step 4, above-mentioned mixed fermentation bacterium solution, 0.08% Tween 80,0.3% that mass percent is 90% are weighed
Calcium chloride, 0.3% sodium metasilicate, 1% chitosan, surplus supply 100% with water；
Step 5, Tween 80, calcium chloride, the silicon weighed in step 4 is added in the mixed fermentation bacterium solution weighed into step 4
Sour sodium, chitosan and water, after being well mixed, that is, obtain postharvest disease and the microbial inoculum of promotion growth before the preventing and treating strawberry is adopted.
Embodiment 3
It is a kind of prevent and treat strawberry adopt before postharvest disease and promote growth microbial inoculum, its raw material by following mass percent component
Composition：95% mixed fermentation bacterium solution, 0.1% Tween 80,0.2% calcium chloride, 0.5% sodium metasilicate, 0.75% shell
Glycan, surplus supplies 100% with water；
Total plate count is 1.0 × 10 in the mixed fermentation bacterium solution11cfu/ml；
The mixed fermentation bacterium solution is by Metschnikowia zizyphicola Y-3 zymocyte liquids, Burkholderia B-1
Zymocyte liquid and enterobacteria B-6-1 zymocyte liquids are according to 1：2：1 mass ratio is mixed；
Specific implementation step is as follows：
Step 1, by Metschnikowia zizyphicola Y-3 pure bacterial strain -80 DEG C take out after at 28 DEG C
48h is cultivated in PDA plate line, and then picking single bacterium is fallen within NYDB fluid nutrient mediums, with 200r/min hunting speed culture
72h, obtains Metschnikowia zizyphicola Y-3 Preliminary fermentation bacterium solution；
By Burkholderia B-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, choose
Take single bacterium to fall within YSP fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain the first of Burkholderia B-1 and originate
Yeast-like fungi liquid；
By enterobacteria B-6-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, choose
Take single bacterium to fall within LB fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain the first of enterobacteria B-6-1 and originate
Yeast-like fungi liquid；
Wherein, NYDB fluid nutrient mediums, YSP fluid nutrient mediums and LB fluid nutrient mediums be the same as Example 1；
Step 2, Metschnikowia zizyphicola Y-3 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums
In, then at 28 DEG C with 200r/min hunting speed cultures to late log phase, obtain Metschnikowia
Zizyphicola Y-3 zymocyte liquids；
Burkholderia B-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain Burkholderia B-1 zymocyte liquids to late log phase；
Enterobacteria B-6-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain enterobacteria B-6-1 zymocyte liquids to late log phase；
Step 3, by the Metschnikowia zizyphicola Y-3 zymocyte liquids, the Burkholderia B- that are obtained in step 2
1 zymocyte liquid and enterobacteria B-6-1 zymocyte liquids are according to 1：2：1 mass ratio mixing, obtains mixed fermentation bacterium solution；
Step 4, weigh mass percent be 95% mixed fermentation bacterium solution, 0.1% Tween 80,0.2% calcium chloride,
0.5% sodium metasilicate, 0.75% chitosan, surplus supply 100% with water；
Step 5, Tween 80, calcium chloride, the silicon weighed in step 4 is added in the mixed fermentation bacterium solution weighed into step 4
Sour sodium, chitosan and water, after being well mixed, that is, obtain postharvest disease and the microbial inoculum of promotion growth before the preventing and treating strawberry is adopted.
The application of postharvest disease and the microbial inoculum of promotion growth is done come the effect to the present invention before being adopted with reference to preventing and treating strawberry
Further instruction.
Because the embodiment 1-3 microbial inoculums prepared are used for spraying after strawberry, the strawberry various performance parameters determined are similar,
Therefore preferred microbial inoculum sprays the performance parameter determined after strawberry and is used as explanation only using in embodiment 2.
First, the preventing and treating of postharvest disease and the microbial inoculum of promotion growth to Diseases of Strawberry before being adopted using the preventing and treating strawberry of embodiment 2
Experiment
Experiment material：Strawberry (kind is " beauty ") is in Yuci City in Shanxi Province's Dongyang town chamber planting, and unused chemistry is killed
Microbial inoculum.
Control sample：Sprayed using sterilized water.
Handle sample：Postharvest disease and the microbial inoculum of promotion growth before the preventing and treating strawberry prepared using embodiment 2 is adopted.
Implementation：Strawberry is sprayed using hand sprayer when preceding 6d, 3d and 0d is adopted, liquid has been sprayed application to
Untill dripping, phase repeat spraying is each sprayed 3 times, 40 plants of strawberries of repetition are sprayed every time, will be sprayed when the Strawberry ripening phase
Strawberry fruit harvesting after transport laboratory back, select the consistent fruit without machinery wound of color and luster and tested, experimental result is as follows：
1st, control of the preharvest spraying microbial inoculum to strawberry fruit gray mold
By the strawberry fruit after above-mentioned harvesting concentration be 1 × 10510s, nothing are dipped in spore/ml Botrytis cinerea suspension
It is loaded into sterilizing plastic casing, plus is sequentially placed after polybag moisturizing in 7d at 6 DEG C 15d and 16 DEG C, note after bacterium wind is dry
Disease incidence is recorded, is repeated 3 times per batch processing, specific experiment result is shown in Fig. 1.
It will be seen from figure 1 that being preserved at 16 DEG C after 7d, the incidence of disease of the strawberry fruit botrytis cinerea handled with microbial inoculum is
32.23%, and the incidence of disease of the strawberry fruit botrytis cinerea of control sample is 93.87%；15d strawberry fruit grey mold is preserved at 6 DEG C
The incidence of bacterium is similar to the situation that 7d is preserved at 16 DEG C, the incidence of disease of strawberry fruit botrytis cinerea after 3 microbial inoculum processing of sprinkling
It is significantly reduced for control sample, it can be seen that, for the strawberry control sample for spraying sterilized water, spray this hair
Bright microbial inoculum can be good at the incidence of disease for controlling to adopt rear strawberry fruit gray mold.
2nd, preharvest spraying microbial inoculum is to strawberry fruit rotten influence naturally
Strawberry fruit after harvesting is fitted into plastic casing, plastic casing moisturizing is positioned at 6 DEG C 7d at 15d and 16 DEG C,
The rotting rate of fruit is recorded, is repeated 3 times per batch processing, specific experiment result is shown in Fig. 2.
As can be seen from Figure 2, after preharvest spraying microbial inoculum, at two different temperatures, the treated strawberry fruit of microbial inoculum is sprayed
Rotting rate is substantially reduced, at 6 DEG C after processing, and rotting rate is 24.14% during 15d, and 16 DEG C, rotting rate is 20.72% during 7d.And
The rotting rate of control sample, at 16 DEG C, is 78.98% during 7d, is 76.73% at 6 DEG C, during 15d.Therefore, answering for the present invention is sprayed
Close microbial inoculum to can be good at preventing strawberry from rotting, reduce the rotting rate of strawberry.
3rd, influence of the preharvest spraying microbial inoculum to strawberry fruit Post-harvest quality
Fruit surface value of chromatism is determined using CR-400 types color difference meter (Minolta, Japan), and wherein L* values represent brightness,
A* value negative values represent green glow, and on the occasion of representing feux rouges, H values represent hue angle, and value of chromatism is entered to determine at fruit equator position, before adopting
Spray microbial inoculum the influence of rear surface chromatic aberration is adopted to strawberry fruit and be shown in Table 1.
Table 1 adopts the influence that rear color and luster change is adopted in preceding composite bacteria agent processing to strawberry
From table 1 it follows that by the sample treated with composite bacteria agent under storage period latter two condition of different temperatures
Product brightness value declines.Compared with control sample, preserved at two temperature after 7d and 15d, although the L* values of processing sample compare control sample
It is slightly higher, but difference is not notable.A* values and H values also obtain similar conclusion, and each processing sample a* values are generally raised after storage, and
H values are generally reduced, and show that fruit color further turns red, and it is unobvious to handle difference between sample and control sample, shows preharvest spraying
Microbial inoculum is not obvious to strawberry fruit storage period Color influences.
Influence of the preharvest spraying microbial inoculum to strawberry quality is shown in Table 2.
Composite bacteria agent handles the influence to strawberry Post-harvest quality before table 2 is adopted
Influence of the preharvest spraying microbial inoculum to strawberry quality be shown in Table at 2,16 DEG C preserve 7d after strawberry fruit weight-loss ratio compared with 6 DEG C
15d is big, but without significant difference under identical temperature conditionss.In addition, preharvest spraying microbial inoculum can delayed fruit to a certain extent
Hardness, SSC, titratable acid, the decline of Vc contents.
2nd, postharvest disease and the microbial inoculum of promotion growth are to strawberry growth effect before being adopted using the preventing and treating strawberry of embodiment 2
Experiment
Experiment material：Strawberry (kind is " beauty ") is in Yuci City in Shanxi Province's Dongyang town chamber planting, and unused chemistry is killed
Microbial inoculum.
Control sample：Sprayed using sterilized water.
Handle sample：Postharvest disease and the microbial inoculum of promotion growth before the preventing and treating strawberry prepared using embodiment 2 is adopted.
Experimentation：At the strawberry best fruiting period (early April), occur more serious plot in powdery mildew and strawberry is entered
Row is sprayed, and 1 microbial inoculum was sprayed every 2 days, is sprayed 3 times altogether.Strawberry is sprayed using hand sprayer when spraying, liquid has been sprayed application to
Untill dripping, 120 plants of strawberries of repetition are sprayed every time, and control is not dealt with.Last time is sprayed after microbial inoculum 10d, observation note
Record control and the strawberry growing state of processing, the plant height of record strawberry, overground part fresh weight, single-strain blade number, and
A situation arises for powdery mildew.
1st, growgh promoting effects of the microbial inoculum to strawberry
Spray and 3 are shown in Table to the growth-promoting effect of strawberry after composite bacteria agent.
The influence that the processing of the microbial inoculum of table 3 grows to Strawberry Seedlings
| Processing | Plant height (cm) | Plant fresh weight | Single-strain blade number |
| Handle sample | 24.26±0.27 | 103.59±2.42 | 7.53±0.28 |
| Control sample | 19.56±0.43 | 89.42±2.83 | 5.31±0.42 |
From table 3 it can be seen that growth of 3 microbial inoculums of preharvest spraying to strawberry shows certain promotion.With it is right
Compared according to plant, the strawberry for spraying 3 times is more healthy and stronger, leaf dark green, growing way is substantially better than control, preharvest spraying 3 times
Plant plant height, plant fresh weight and single-strain blade number are significantly increased.
2nd, inhibitory action of the microbial inoculum to powdery mildew
Spray after microbial inoculum agent 10d, will handle and control is all harvested into the strawberry after turn red phase, and count respectively careless
The incidence of disease of certain kind of berries fruit and plant powdery mildew, experimental result is shown in Table 4.
Influence of the microbial inoculum of table 4 processing to Strawberry Seedlings powdery mildew
| Processing | The plant incidence of disease | The fruit incidence of disease |
| Handle sample | 12.97±1.42 | 56.43±2.18 |
| Control sample | 27.52±1.35 | 29.08±1.53 |
From table 4, it can be seen that the plant incidence of disease of processing sample and fruit incidence of disease bacterium are significantly lower than control sample, spray compound
The effect of the Strawberry Seedlings suppression powdery mildew incidence of disease is obvious after microbial inoculum.
, but those skilled in the art once know basic creation although preferred embodiments of the present invention have been described
Property concept, then can make other change and modification to these embodiments.So, appended claims are intended to be construed to include excellent
Select embodiment and fall into having altered and changing for the scope of the invention.
Obviously, those skilled in the art can carry out the essence of various changes and modification without departing from the present invention to the present invention
God and scope.So, if these modifications and variations of the present invention belong to the scope of the claims in the present invention and its equivalent technologies
Within be also intended to comprising these change and modification including.
Claims (7)
1. a kind of prevent and treat postharvest disease and the microbial inoculum of promotion growth before strawberry is adopted, it is characterised in that its raw material is by following quality hundred
Divide the component composition of ratio：85~95% mixed fermentation bacterium solution, 0.05~0.1% Tween 80,0.2~0.5% calcium chloride,
0.1~0.5% sodium metasilicate, 0.75~2% chitosan, surplus are supplied with water to 100%；
Total plate count in the mixed fermentation bacterium solution is 1.0 × 106~1.0 × 1011cfu/mL；
The mixed fermentation bacterium solution is by the strange yeast Y-3 of plum (Metschnikowia chrysoperlae Y-3) zymocyte liquid, primary
Kirschner bacterium B-1 (Burkholderia contaminans B-1) zymocyte liquids and enterobacteria B-6-1
(EnterobactercowaniiB-6-1) zymocyte liquid is according to 1：1~2：0.5~1 mass ratio is mixed；
Wherein, the strange yeast Y-3 zymocyte liquids of the plum are obtained by the strange pure strain culturings of yeast Y-3 of plum to late log phase；It is described
Burkholderia B-1 zymocyte liquids are obtained by the pure strain culturings of Burkholderia B-1 to late log phase；The enterobacteria B-6-1
Zymocyte liquid is obtained by the pure strain culturings of enterobacteria B-6-1 to late log phase.
Postharvest disease and the microbial inoculum of promotion growth before 2. preventing and treating strawberry according to claim 1 is adopted, it is characterised in that it is former
Material is made up of the component of following mass percent：90% mixed fermentation bacterium solution, 0.08% Tween 80,0.3% calcium chloride,
0.3% sodium metasilicate, 1% chitosan, surplus are supplied with water to 100%；
Total plate count is 1.0 × 10 in the mixed fermentation bacterium solution8cfu/ml；
The mixed fermentation bacterium solution is by the strange yeast Y-3 zymocyte liquids of plum, Burkholderia B-1 zymocyte liquids and enterobacteria B-6-1
Zymocyte liquid is according to 1：1：0.5 mass ratio is mixed.
The preparation method of postharvest disease and the microbial inoculum for promoting growth before 3. preventing and treating strawberry according to claim 1 is adopted, it is special
Levy and be, comprise the following steps：
Step 1, after cultivating 48h in PDA plate line at 28 DEG C, then the strange yeast Y-3 of plum pure bacterial strain is taken out at -80 DEG C
Picking single bacterium is fallen within NYDB fluid nutrient mediums, with 200r/min hunting speed culture 72h, obtains the first of the strange yeast Y-3 of plum
Beginning zymocyte liquid；
By Burkholderia B-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, picking single bacterium
Fall within YSP fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain Burkholderia B-1 Preliminary fermentation bacterium
Liquid；
By enterobacteria B-6-1 pure bacterial strain -80 DEG C take out after at 28 DEG C PDA plate line culture 48h after, picking single bacterium
Fall within LB fluid nutrient mediums, with 200r/min hunting speed culture 24h, obtain enterobacteria B-6-1 Preliminary fermentation bacterium solution；
Step 2, the strange yeast Y-3 of plum Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then with 200r/ at 28 DEG C
Min hunting speed cultures obtain the strange yeast Y-3 zymocyte liquids of plum to late log phase；
Burkholderia B-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then shaken at 28 DEG C with 200r/min
Speed culture is swung to late log phase, obtains Burkholderia B-1 zymocyte liquids；
Enterobacteria B-6-1 Preliminary fermentation bacterium solution is placed in NYDB fluid nutrient mediums, then shaken at 28 DEG C with 200r/min
Speed culture is swung to late log phase, obtains enterobacteria B-6-1 zymocyte liquids；
Step 3, by the strange yeast Y-3 zymocyte liquids of the plum obtained in step 2, Burkholderia B-1 zymocyte liquids and enterobacteria B-
6-1 zymocyte liquids press 1：1~2：0.5~1 mass ratio mixing, obtains mixed fermentation bacterium solution；
Step 4, weigh mass percent be 85~95% above-mentioned mixed fermentation bacterium solution, 0.05%~0.1% Tween 80,
0.2~0.5% calcium chloride, 0.1~0.5% sodium metasilicate, 0.75%~2% chitosan, surplus supply 100% with water, standby
With；
Step 5, in the mixed fermentation bacterium solution weighed into step 4 add step 4 in weigh Tween 80, calcium chloride, sodium metasilicate,
Chitosan and water, after being well mixed, that is, obtain postharvest disease and the microbial inoculum of promotion growth before the preventing and treating strawberry is adopted.
The preparation method of postharvest disease and the microbial inoculum for promoting growth before 4. preventing and treating strawberry according to claim 3 is adopted, it is special
Levy and be, following components are included in every liter of YSP fluid nutrient medium：Peptone 10g, yeast extract 5g, glucose 10g, surplus
For water.
The preparation method of postharvest disease and the microbial inoculum for promoting growth before 5. preventing and treating strawberry according to claim 4 is adopted, it is special
Levy and be, following components are included in every liter of NYDB fluid nutrient medium：Beef extract 8g, yeast extract 5g, glucose 10g, it is remaining
Measure as water.
The preparation method of postharvest disease and the microbial inoculum for promoting growth before 6. preventing and treating strawberry according to claim 5 is adopted, it is special
Levy and be, following components are included in every liter of LB fluid nutrient medium：Tryptone 10g, yeast extract 5g, NaCl 10g,
Surplus is water.
7. application of the microbial inoculum according to claim 1 in postharvest disease preventing and treating before strawberry grows and adopted.
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| CN108077319A (en) * | 2018-01-16 | 2018-05-29 | 河北省农林科学院石家庄果树研究所 | A kind of biological and ecological methods to prevent plant disease, pests, and erosion fermented liquid for preventing fruit tree putrefaction disease and preparation method thereof |
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