CN106358753A - Inoculation process for edible fungi - Google Patents

Inoculation process for edible fungi Download PDF

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Publication number
CN106358753A
CN106358753A CN201610731016.9A CN201610731016A CN106358753A CN 106358753 A CN106358753 A CN 106358753A CN 201610731016 A CN201610731016 A CN 201610731016A CN 106358753 A CN106358753 A CN 106358753A
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CN
China
Prior art keywords
carrying
weight portion
bacterium
temperature
edible fungi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610731016.9A
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Chinese (zh)
Inventor
曹光标
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fuzhou Kaida Ecological Agriculture Co Ltd
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Fuzhou Kaida Ecological Agriculture Co Ltd
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Filing date
Publication date
Application filed by Fuzhou Kaida Ecological Agriculture Co Ltd filed Critical Fuzhou Kaida Ecological Agriculture Co Ltd
Priority to CN201610731016.9A priority Critical patent/CN106358753A/en
Publication of CN106358753A publication Critical patent/CN106358753A/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C9/00Fertilisers containing urea or urea compounds

Abstract

The invention relates to an inoculation process for edible fungi. The process comprises the following steps: (1) carrying out crushing: mixing wood sawdust, cottonseed hulls, bamboo sawdust and wheat bran, and carrying out crushing, so as to obtain a powder mixture; (2) carrying out low-temperature baking; (3) carrying out raw material stirring: adding ingredients, i.e., lime powder, gypsum, urea and a compound fertilizer into the dried powder mixture obtained in the step (2), and carrying out mixing and stirring, so as to obtain a secondary mixture; (4) carrying out bagging; (5) carrying out quick-freezing sterilization; (6) carrying out perforation and high-temperature steam sterilization, and regulating the moisture content of the bagged mixture; (7) carrying out temperature adjustment; (8) carrying out inoculation; and (9) carrying out inoculum culture. Tremella fuciformis produced by the process has the advantages of high quality, high efficiency, increased yield and low cost.

Description

A kind of inoculating process of edible fungi
Technical field
The present invention relates to a kind of inoculating process of edible fungi, belong to edible fungi inoculation field.
Background technology
Tremella is also referred to as Tremella, Tremella, Tremella etc., belongs to Mycophyta Tremellaceae Tremella, is that a Basidiomycota is true The sporophore of bacterium Tremella, has the laudatory title of " hat in bacterium ".Tremella fructification pure white to milky, 5~10 centimetres of diameter, soft clean In vain, translucent, high resilience.Tremella sweet in the mouth, light, mild-natured, nontoxic, effect of existing spleen reinforcing appetizing, have QI invigorating bowel relieving, YIN nourishing again The effect of lung moistening.Body immunity can be strengthened, the tolerance to Radiotherapy chemotherapy for the tumor patient can be strengthened again.Tremella is rich in natural Vegetalitas colloid, additional its has the effect of YIN nourishing, is can be with the good skin moistening food of long-term taking.
Therefore, for those skilled in the art, a kind of formula material of suitable Tremella growth of exploitation and inoculating process are needed badly.
Content of the invention
In view of the above problems, the technical problem to be solved in the present invention is to provide a kind of method of Tremella inoculation, produces The Tremella mycoplasma amount come is good, efficiency is fast, low cost.
For solving above-mentioned technical problem, a kind of cultural method of Tremella that the present invention provides, comprise the following steps:
1. pulverize: the wood flour of following weight portion, cotton seed hullss, bamboo scraps and wheat bran are pressed and pulverizes after proportioning mixing, obtain powder mixing Material;
Wood flour 52 weight portion
Cotton seed hullss 23 weight portion
Bamboo scraps 20 weight portion
Wheat bran 3 weight portion
2. oven drying at low temperature: the powder mixture oven drying at low temperature 3-5 hour of step 1. gained is obtained dry pack;
3. raw material stirring: the pulverized limestone of following weight portion, Gypsum Fibrosum, carbamide and compound fertilizer are pressed and adds step after proportioning mix and blend 2. the dry pack obtaining obtains secondary mixed material;
Pulverized limestone 0.8 weight portion
Gypsum Fibrosum 0.5 weight portion
Carbamide 0.1 weight portion
Compound fertilizer 0.1 weight portion
4. pack: the secondary mixed material that 3. step is obtained loads a diameter of 16 centimetres, and length is in 30 centimetres of bacterium bag, and presses Flat is become to obtain packed compound;
5. IQF sterilization: the packed compound of step 4. gained is put into IQF case, 5 minutes will at a temperature of Drop to subzero 100 DEG C, keep 30 minutes;
6. high-temp steam sterilizing, symmetrically opens 8 holes, and adjusts and seal 8 holes with adhesive tape immediately after water content reaches 60%;
7. homoiothermic: be cooled to 30 DEG C;
8. inoculate: inoculated in transfer room, carry out asepticization process in advance in transfer room, extract strain with inoculator, concentrate Run through alcohol burner flame to access in cave;During inoculation, it is respectively connected to Tremella bacterium, incense ashes bacterium and cellulomonas cartae;
9. spawn culture: put into culturing room and carry out sending out bacterium culture 13 days, at 25 DEG C, air humidity is maintained at culturing room's temperature control 80%, culturing room is carried out disinfection sterilization treatment with disinfectant before spawn culture, carried out a miscellaneous bacteria every 3 days and check and send out The inspection of bacterium situation, in morning and evening ventilation when temperature is high, it is to avoid sunlight irradiates.
The invention has the following beneficial effects:
1st, the present invention adds bamboo scraps in the feed, and bamboo product leftover bits and pieces can be had to obtain, and belongs to garbage and recycles, resource is fully sharp With reducing production cost;
2nd, add incense ashes bacterium as the concomitance bacterium of Tremella bacterium in the present invention, the ingredient breakdown that can cannot absorb Tremella bacterium becomes Tremella The absorbent nutrient of bacterium;
3rd, add fungin can be decomposed bamboo and wood fiber in the present invention, promote the growth such as Tremella;
4th, the present invention adopts IQF to sterilize, and can achieve sterilization more thoroughly, especially in the middle part of compound;
5th, the present invention adopts high-temperature steam re-pasteurization, can sterilize further;
6th, the present invention adjusts water content by high-temperature steam, and early stage reduces water content and can prolong the compound shelf lives of early stage Long, be conducive to the operation producing.
Specific embodiment
To further describe the present invention referring to specific embodiment, to make those skilled in the art with reference to description literary composition Word can be implemented according to this, and the scope of the present invention is not limited to embodiments of the present invention.
1. pulverize: the wood flour of following weight portion, cotton seed hullss, bamboo scraps and wheat bran are pressed and pulverizes after proportioning mixing, obtain powder Compound;
Wood flour 52 weight portion
Cotton seed hullss 23 weight portion
Bamboo scraps 20 weight portion
Wheat bran 3 weight portion
2. oven drying at low temperature: the powder mixture oven drying at low temperature 3-5 hour of step 1. gained is obtained dry pack;
3. raw material stirring: the pulverized limestone of following weight portion, Gypsum Fibrosum, carbamide and compound fertilizer are pressed and adds step after proportioning mix and blend 2. the dry pack obtaining obtains secondary mixed material;
Pulverized limestone 0.8 weight portion
Gypsum Fibrosum 0.5 weight portion
Carbamide 0.1 weight portion
Compound fertilizer 0.1 weight portion
4. pack: the secondary mixed material that 3. step is obtained loads a diameter of 16 centimetres, and length is in 30 centimetres of bacterium bag, and presses Flat is become to obtain packed compound;
5. IQF sterilization: the packed compound of step 4. gained is put into IQF case, 5 minutes will at a temperature of Drop to subzero 100 DEG C, keep 30 minutes;
6. high-temp steam sterilizing, symmetrically opens 8 holes, and adjusts and seal 8 holes with adhesive tape immediately after water content reaches 60%;
7. homoiothermic: be cooled to 30 DEG C;
8. inoculate: inoculated in transfer room, carry out asepticization process in advance in transfer room, extract strain with inoculator, concentrate Run through alcohol burner flame to access in cave;During inoculation, it is respectively connected to Tremella bacterium, incense ashes bacterium and cellulomonas cartae;
9. spawn culture: put into culturing room and carry out sending out bacterium culture 13 days, at 25 DEG C, air humidity is maintained at culturing room's temperature control 80%, culturing room is carried out disinfection sterilization treatment with disinfectant before spawn culture, carried out a miscellaneous bacteria every 3 days and check and send out The inspection of bacterium situation, in morning and evening ventilation when temperature is high, it is to avoid sunlight irradiates.
The foregoing is only embodiments of the invention, not thereby limit the present invention the scope of the claims, every using this The equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technical fields, all wrap in the same manner Include in the scope of patent protection of the present invention.

Claims (2)

1. a kind of inoculating process of edible fungi it is characterised in that:
1. pulverize: the wood flour of following weight portion, cotton seed hullss, bamboo scraps and wheat bran are pressed and pulverizes after proportioning mixing, obtain powder mixing Material;
Wood flour 52 weight portion,
Cotton seed hullss 23 weight portion,
Bamboo scraps 20 weight portion,
Wheat bran 3 weight portion;
2. oven drying at low temperature: the powder mixture oven drying at low temperature 3-5 hour of step 1. gained is obtained dry pack;
3. raw material stirring: the pulverized limestone of following weight portion, Gypsum Fibrosum, carbamide and compound fertilizer are pressed and adds step after proportioning mix and blend 2. the dry pack obtaining obtains secondary mixed material;
Pulverized limestone 0.8 weight portion,
Gypsum Fibrosum 0.5 weight portion,
Carbamide 0.1 weight portion,
Compound fertilizer 0.1 weight portion;
4. pack: the secondary mixed material that 3. step is obtained loads a diameter of 16 centimetres, and length is in 30 centimetres of bacterium bag, and presses Flat is become to obtain packed compound;
5. IQF sterilization: the packed compound of step 4. gained is put into IQF case, 5 minutes will at a temperature of Drop to subzero 100 DEG C, keep 30 minutes;
6. high-temp steam sterilizing, symmetrically opens 8 holes, and adjusts and seal 8 holes with adhesive tape immediately after water content reaches 60%;
7. homoiothermic: be cooled to 30 DEG C;
8. inoculate: inoculated in transfer room, carry out asepticization process in advance in transfer room, extract strain with inoculator, concentrate Run through alcohol burner flame to access in cave;During inoculation, it is respectively connected to Tremella bacterium, incense ashes bacterium and cellulomonas cartae;
9. spawn culture: put into culturing room and carry out sending out bacterium culture 13 days, at 25 DEG C, air humidity is maintained at culturing room's temperature control 80%, carried out a miscellaneous bacteria every 3 days and check and send out the inspection of bacterium situation, in morning and evening ventilation when temperature is high, it is to avoid sunlight shines Penetrate.
2. edible fungi according to claim 1 inoculating process it is characterised in that: with disappearing before step 9. spawn culture Toxic agent carries out disinfection to culturing room sterilization treatment.
CN201610731016.9A 2016-08-26 2016-08-26 Inoculation process for edible fungi Pending CN106358753A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610731016.9A CN106358753A (en) 2016-08-26 2016-08-26 Inoculation process for edible fungi

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Application Number Priority Date Filing Date Title
CN201610731016.9A CN106358753A (en) 2016-08-26 2016-08-26 Inoculation process for edible fungi

Publications (1)

Publication Number Publication Date
CN106358753A true CN106358753A (en) 2017-02-01

Family

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109168961A (en) * 2018-10-31 2019-01-11 芒市喜丰农业发展有限公司 A kind of cultural method of white ginseng

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102696401A (en) * 2012-07-03 2012-10-03 韶关学院 Factorial method for cultivating white fungi
CN102771313A (en) * 2012-08-15 2012-11-14 苏州硒谷科技有限公司 Method for culturing selenium-enriched agarics by utilizing selenium-enriched crop straws
CN102972210A (en) * 2012-12-12 2013-03-20 张德品 New tremella cultivation method
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CN103626553A (en) * 2013-12-03 2014-03-12 邬金梅 Method utilizing sunflower by-products to manufacture white fungus cultivation material
CN103798057A (en) * 2014-02-19 2014-05-21 韦承梭 Tremella cultivation medium and tremella cultivation method
CN103880537A (en) * 2014-03-26 2014-06-25 泗阳县农业科学研究所 Culture medium and cultivation method of tremella fuciformis
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CN102696401A (en) * 2012-07-03 2012-10-03 韶关学院 Factorial method for cultivating white fungi
CN102771313A (en) * 2012-08-15 2012-11-14 苏州硒谷科技有限公司 Method for culturing selenium-enriched agarics by utilizing selenium-enriched crop straws
CN102972210A (en) * 2012-12-12 2013-03-20 张德品 New tremella cultivation method
CN103355094A (en) * 2013-07-10 2013-10-23 重庆市中药研究院 Method of cultivating tremella by utilizing mulberry twig
CN103626553A (en) * 2013-12-03 2014-03-12 邬金梅 Method utilizing sunflower by-products to manufacture white fungus cultivation material
CN103798057A (en) * 2014-02-19 2014-05-21 韦承梭 Tremella cultivation medium and tremella cultivation method
CN103880537A (en) * 2014-03-26 2014-06-25 泗阳县农业科学研究所 Culture medium and cultivation method of tremella fuciformis
CN104557201A (en) * 2014-12-29 2015-04-29 河南牧业经济学院 Selenium-rich white fungus cultivation material and preparation method thereof
CN105036920A (en) * 2015-07-22 2015-11-11 福建信龙农产品开发有限公司 Novel tremella culture medium and tremella cultivation method
CN105850510A (en) * 2016-05-20 2016-08-17 灵璧县恒利健食用菌种植有限公司 Agaric cultivating method

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Publication number Priority date Publication date Assignee Title
CN109168961A (en) * 2018-10-31 2019-01-11 芒市喜丰农业发展有限公司 A kind of cultural method of white ginseng

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Application publication date: 20170201