CN106353143A - Device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection - Google Patents
Device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection Download PDFInfo
- Publication number
- CN106353143A CN106353143A CN201611002797.4A CN201611002797A CN106353143A CN 106353143 A CN106353143 A CN 106353143A CN 201611002797 A CN201611002797 A CN 201611002797A CN 106353143 A CN106353143 A CN 106353143A
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- Prior art keywords
- detection
- antigen elisa
- dog
- filter tube
- supernatant collection
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 239000000427 antigen Substances 0.000 title claims abstract description 23
- 102000036639 antigens Human genes 0.000 title claims abstract description 23
- 108091007433 antigens Proteins 0.000 title claims abstract description 23
- 238000002965 ELISA Methods 0.000 title abstract description 5
- 210000003608 fece Anatomy 0.000 title abstract 5
- 239000010871 livestock manure Substances 0.000 title abstract 5
- 239000006228 supernatant Substances 0.000 claims description 33
- 239000004033 plastic Substances 0.000 claims description 12
- 229920003023 plastic Polymers 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 6
- 229920003002 synthetic resin Polymers 0.000 claims description 5
- 239000000057 synthetic resin Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 8
- 238000005119 centrifugation Methods 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 7
- 238000005406 washing Methods 0.000 description 6
- 239000000377 silicon dioxide Substances 0.000 description 5
- 241000244160 Echinococcus Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 238000005213 imbibition Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000007781 pre-processing Methods 0.000 description 3
- 238000002203 pretreatment Methods 0.000 description 3
- 239000012898 sample dilution Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009790 rate-determining step (RDS) Methods 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection. The device comprises a filtering tube and an outer-layer casing, wherein holes are formed in the filtering tube; the filtering tube is arranged inside the outer-layer casing. When being used, the outer-layer casing is filled with a dog manure sample and a diluent; after being sufficiently and uniformly mixed, the dog manure sample and the diluent are not centrifuged or centrifuged for 10 minutes at a speed of 3000rpm/min, subsequently, the filtering tube is added into the outer-layer casing, and then the supernate can be directly detected or is sucked out for detection. The device can be adopted to collect the supernate after centrifugation in the pretreatment process of the dog manure sample, the supernate is not affected by floating impurities in the sample, the supernate can be collected without obstacle, the testing process can be accelerated, and the labor and the time can be saved.
Description
Technical field
The present invention relates to a kind of experiment detection device, more particularly, to one kind is for dog stool antigen enzyme-linked immunosorbent assay
(elisa) device of supernatant collection in detecting.
Background technology
Elisa experiment is combined into ultimate principle with the immunology of antigen-antibody, has been widely used in antigen or antibody thing
The mensure of matter, is particularly applied successfully to multiple viruses, the infectious disease caused by antibacterials and parasite and other and has biological point
The immunology diagnosiss of the aspects such as the disease of son identification thing.Elisa method, in addition to having susceptiveness and the preferable feature of specificity, operates
Process is simple and quick, is easy to automatization, at present existing full-automatic elisa detecting system.So that a small-size laboratory
Application elisa method can check hundreds of or even thousands of part of specimen within one day it is adaptable to the detection of clinical samples and people (animal)
Group's seroepidemiological survey.
At present, the usual experimental procedure of elisa experiment be the pre-treatment of specimen, reagent prepare, be loaded, incubating, wash plate,
Colour developing, colorimetric, result judge and the aspect such as result report and explanation, at present in addition to the pre-treatment and reagent preparation of specimen, other
Step all can be operated by self-reacting device and complete.For large sample detection limit laboratory, its rate-limiting step is mainly before specimen
Process.For dog excrement sample, because impurity is more, pre-treatment is more complicated, after needing broken and diluent to be ground, being centrifuged
Take the steps such as supernatant, occupy relatively large number of detection time and manpower.In Aspirate supernatant, dog excrement sample often has
Scum silica frost, leads to imbibition process slower.
Content of the invention
The imbibition obstacle leading to for scum silica frost when overcoming Sample pretreatment Aspirate supernatant in dog excrement sample elisa experiment,
The invention provides a kind of for dog stool antigen elisa detection in supernatant collection device, can be used for dog excrement Sample pretreatment
During centrifuged supernatant collection, do not affected by scum silica frost in sample, make supernatant collection accessible, accelerate test into
Journey, saves manpower and time.
The present invention is achieved by the following technical solutions:
The device of supernatant collection in a kind of detection for dog stool antigen elisa, including filter tube with holes and outer layer sleeve,
Described filter tube is inserted in outer layer sleeve.
As currently preferred technical scheme, material the appointing in glass, plastics, synthetic resin of described filter tube
A kind of.
As currently preferred technical scheme, described filter tube is stereoplasm tube, non-deformable.
As currently preferred technical scheme, described filter tube is less than 2cm for caliber, pipe range is more than the straight tube of 5cm, excellent
Elect as caliber be 0.6～1.5cm, length be 5～10cm straight tube.
As currently preferred technical scheme, the tube wall of described filter tube has the multiple little of 50-300 μm of aperture
Hole.
As currently preferred technical scheme, described aperture is distributed in the bottom of filter tube, and little hole number is 5-50.
As currently preferred technical scheme, the material of described outer layer sleeve is arbitrary in plastics and synthetic resin
Kind.
As currently preferred technical scheme, described outer layer sleeve is deformable flexible pipe.
As currently preferred technical scheme, the caliber of described outer layer sleeve is 1～1.5cm, pipe range 8-12cm, can hold
By the centrifugal force of 3000rpm/min, do not crush.
During use, add dog excrement sample and diluent in outer layer sleeve, after fully mixing, be not centrifuged or 3000rpm/min
Centrifugation 10min after, filter tube is added in outer layer sleeve, then direct detection or by supernatant suction out detection.
Compared with prior art, the beneficial effects of the present invention is: apparatus of the present invention can be used for dog excrement Sample pretreatment mistake
The collection of centrifuged supernatant in journey, is not affected by scum silica frost in sample, makes supernatant collection accessible, accelerates experiment process,
Save manpower and time, the imbibition that when overcoming Sample pretreatment Aspirate supernatant in dog excrement sample elisa experiment, scum silica frost leads to
Obstacle, overcomes the rate-limiting step in original detection technique, the open-assembly time to sample during reducing the personnel to operate, reduces personnel
Exposure to pathogen.Present configuration is simple, and low cost of manufacture, using easy and simple to handle.
Brief description
Fig. 1 is the structural representation for the device of supernatant collection in dog stool antigen elisa detection of the present invention.
In Fig. 1, description of reference numerals is as follows:
1 is filter tube, and 2 is outer layer sleeve, and 3 is aperture.
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention is further illustrated.
As shown in figure 1, the present invention a kind of for dog stool antigen elisa detection in supernatant collection device, including with holes
Filter tube 1, described filter tube 1, preferably caliber 0.6～1.5cm, length are the straight tube of 5～10cm, using glass, plastics,
Any one material in synthetic resin is prepared from, and is stereoplasm tube, non-deformable.Aperture 50- is had on the tube wall of filter tube 1
300 μm of aperture 3, aperture 3 is perfectly even distributed in the bottom of filter tube, and the quantity of aperture 3 is 5-50.
In a kind of described dog stool antigen elisa detection, the device of supernatant collection also includes outer layer sleeve 2, filter tube 1
Can insert in outer layer sleeve 2.The material of outer layer sleeve 2 is deformable plastic flexible pipe, caliber 1～1.5cm, pipe range 8-12cm,
The centrifugal force of 3000rpm/min can be born, do not crush.
During use, add dog excrement sample and diluent in outer layer sleeve 2, after fully mixing, be not centrifuged or 3000rpm/min
Centrifugation 10min after, filter tube 1 is added in outer layer sleeve 2, then direct detection or by supernatant suction out detection.
Embodiment 1 dog excrement echinococcus antigen elisa detection Sample pretreatment is without the direct supernatant collection of centrifugation
Step:
1. dog excrement sample preprocessing: (- 70 DEG C) of low temperature freezes 14 days dog excrement sample in advance.
2. concentrating sample diluent deionized water is pressed 1:1 dilution, fully mix and be sample diluting liquid.
3. weigh dog excrement sample 1g and add caliber 1cm, in the deformable plastic flexible pipe of pipe range 10cm (i.e. outer layer sleeve 2),
Add 1ml Sample dilution, be sufficiently stirred for mixing, stand 10min.
4. by the bottom of the caliber 0.9cm being prepared from using plastics, the hard straight type filter tube 1(filter tube 1 of pipe range 8cm
The aperture 3 in 100 μm of aperture is had on portion's tube wall, the quantity of aperture 3 is 30) put in outer layer sleeve 2, see Fig. 1.
5. draw 100 μ l supernatant sample-adding, washing, enzyme combination, washing successively to be described, shows according to elisa producer test kit
Color, termination measured value and result judgement.
Direct supernatant collection after embodiment 2 dog excrement echinococcus antigen elisa detection Sample pretreatment centrifugation
1. dog excrement sample preprocessing: (- 70 DEG C) of low temperature freezes 14 days dog excrement sample in advance.
2. concentrating sample diluent deionized water is pressed 1:1 dilution, fully mix and be sample diluting liquid.
3. weigh dog excrement sample 1g and add caliber 1.2cm, the deformable plastic flexible pipe of pipe range 10cm (i.e. outer layer sleeve 2)
In, add 1ml Sample dilution, be sufficiently stirred for mixing, 3000rpm/min is centrifuged 10min.
4. by the bottom of the caliber 1.1cm being prepared from using plastics, the hard straight type filter tube 1(filter tube 1 of pipe range 6cm
The aperture 3 in 100 μm of aperture is had on portion's tube wall, the quantity of aperture 3 is 30) put in outer layer sleeve 2, see Fig. 1.
5. draw 100 μ l supernatant sample-adding, washing, enzyme combination, washing successively to be described, shows according to elisa producer test kit
Color, termination measured value and result judgement.
Embodiment 3 dog excrement echinococcus antigen elisa detection Sample pretreatment centrifuged supernatant is drawn and is collected
1. dog excrement sample preprocessing: (- 70 DEG C) of low temperature freezes 14 days dog excrement sample in advance.
2. concentrating sample diluent deionized water is pressed 1:1 dilution, fully mix and be sample diluting liquid.
3. weigh dog excrement sample 1g and add caliber 1.5cm, in the deformable plastic flexible pipe of pipe range 10cm (outer layer sleeve 2),
Add 1ml Sample dilution, be sufficiently stirred for mixing, 3000rpm/min is centrifuged 10min.
4. by the bottom of the caliber 1.4cm being prepared from using plastics, the hard straight type filter tube 1(filter tube 1 of pipe range 7cm
The aperture 3 in 100 μm of aperture is had on portion's tube wall, the quantity of aperture 3 is 30) put in outer layer sleeve 2, see Fig. 1.
5. draw 100 μ l supernatant sample-adding, washing, enzyme combination, washing successively to be described, shows according to elisa producer test kit
Color, termination measured value and result judgement.
With the above-mentioned desirable embodiment according to the present invention for enlightenment, by above-mentioned description, relevant staff is complete
Entirely various change and modification can be carried out in the range of without departing from this invention technological thought.The technology of this invention
The content that property scope is not limited in description it is necessary to determine its technical scope according to right.
Claims (10)
1. in a kind of detection for dog stool antigen elisa supernatant collection device it is characterised in that: include filter tube with holes
And outer layer sleeve, described filter tube inserts in outer layer sleeve.
2. in a kind of detection for dog stool antigen elisa according to claim 1 supernatant collection device, its feature exists
In: any one in glass, plastics, synthetic resin of the material of described filter tube.
3. in a kind of detection for dog stool antigen elisa according to claim 1 supernatant collection device, its feature exists
In: described filter tube is stereoplasm tube, non-deformable.
4. in a kind of detection for dog stool antigen elisa according to claim 1 supernatant collection device, its feature exists
In: described filter tube is less than 2cm for caliber, pipe range is more than the straight tube of 5cm.
5. in a kind of detection for dog stool antigen elisa according to claim 4 supernatant collection device, its feature exists
In: described filter tube for caliber be 0.6～1.5cm, length be 5～10cm straight tube.
6. in a kind of detection for dog stool antigen elisa according to claim 1 supernatant collection device, its feature exists
In: multiple apertures in 50-300 μm of aperture are had on the tube wall of described filter tube.
7. in a kind of detection for dog stool antigen elisa according to claim 6 supernatant collection device, its feature exists
In: described aperture is distributed in the bottom of filter tube, and little hole number is 5-50.
8. in a kind of detection for dog stool antigen elisa according to claim 1 supernatant collection device, its feature exists
In: the material of described outer layer sleeve is selected from any one in plastics and synthetic resin.
9. in a kind of detection for dog stool antigen elisa according to claim 1 or 8 supernatant collection device, it is special
Levy and be: described outer layer sleeve is deformable flexible pipe.
10., according to the device of supernatant collection in a kind of detection for dog stool antigen elisa described in claim 1, its feature exists
In: the caliber of described outer layer sleeve is 1～1.5cm, pipe range 8-12cm, can bear the centrifugal force of 3000rpm/min, not crush.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611002797.4A CN106353143A (en) | 2016-11-15 | 2016-11-15 | Device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611002797.4A CN106353143A (en) | 2016-11-15 | 2016-11-15 | Device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106353143A true CN106353143A (en) | 2017-01-25 |
Family
ID=57863080
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611002797.4A Pending CN106353143A (en) | 2016-11-15 | 2016-11-15 | Device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection |
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| Country | Link |
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| CN (1) | CN106353143A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108519338A (en) * | 2018-02-12 | 2018-09-11 | 云南中烟工业有限责任公司 | A kind of high-throughput Continuous Flow Analysis method for water-soluble sugar in tobacco gene editor's material |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994015703A1 (en) * | 1993-01-14 | 1994-07-21 | Scimat Limited | A filter |
| TWM292601U (en) * | 2005-12-06 | 2006-06-21 | Da-De Jang | Structure of tubular material |
| CN203154940U (en) * | 2013-04-08 | 2013-08-28 | 李涛 | Punching filter tube |
| CN203705198U (en) * | 2013-12-17 | 2014-07-09 | 深圳华大基因研究院 | Device and system for collecting and processing saliva sample |
| CN203999615U (en) * | 2014-07-25 | 2014-12-10 | 何伟 | A kind of trace amount DNA extraction centrifuge shield |
| CN206470083U (en) * | 2016-11-15 | 2017-09-05 | 中国疾病预防控制中心寄生虫病预防控制所 | The device of supernatant collection in a kind of detection for dog stool antigen ELISA |
-
2016
- 2016-11-15 CN CN201611002797.4A patent/CN106353143A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994015703A1 (en) * | 1993-01-14 | 1994-07-21 | Scimat Limited | A filter |
| TWM292601U (en) * | 2005-12-06 | 2006-06-21 | Da-De Jang | Structure of tubular material |
| CN203154940U (en) * | 2013-04-08 | 2013-08-28 | 李涛 | Punching filter tube |
| CN203705198U (en) * | 2013-12-17 | 2014-07-09 | 深圳华大基因研究院 | Device and system for collecting and processing saliva sample |
| CN203999615U (en) * | 2014-07-25 | 2014-12-10 | 何伟 | A kind of trace amount DNA extraction centrifuge shield |
| CN206470083U (en) * | 2016-11-15 | 2017-09-05 | 中国疾病预防控制中心寄生虫病预防控制所 | The device of supernatant collection in a kind of detection for dog stool antigen ELISA |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108519338A (en) * | 2018-02-12 | 2018-09-11 | 云南中烟工业有限责任公司 | A kind of high-throughput Continuous Flow Analysis method for water-soluble sugar in tobacco gene editor's material |
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