CN106350455A - Hyphae pellet mediated fast microalga harvesting method - Google Patents

Hyphae pellet mediated fast microalga harvesting method Download PDF

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CN106350455A
CN106350455A CN201610827066.7A CN201610827066A CN106350455A CN 106350455 A CN106350455 A CN 106350455A CN 201610827066 A CN201610827066 A CN 201610827066A CN 106350455 A CN106350455 A CN 106350455A
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microalgae
culture
pellet
fungi
culture medium
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周文广
李晶晶
黎俊
陈杰
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Nanchang University
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Nanchang University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00—Spore forming or isolating processes

Abstract

The invention discloses a hyphae pellet mediated fast microalga harvesting method, including steps of (1) transferring microalgae; (2) culturing the microalgae under high density; (3) culturing balling fungal spore; (4) forming and culturing mycelium pellets; (5) transferring the mycelium pellets to microalga culture solution; (6) culturing the mycelium pellets during the microalga absorbing process; (7) harvesting the mycelium pellets of the microalgae by filtering. Through introducing the balling hyphae pellets into the microalga culture solution to be harvested and mixing and culturing, rapidly absorbing microalgae by mycelium pellets can be realized; finally, the mycelium pellets of microalgae can be harvested through simple filtering method.

Description

A kind of quick harvesting method of microalgae of fungi pellet mediation
Technical field
The invention belongs to microalgae recovery field, it is related to a kind of quick collecting method of microalgae of fungi pellet mediation.Specifically It is exactly to allow microalgae cell grow under the conditions of photoautotrophy or heterotrophism first in fact；Then the different times in cell growth import Fungi pellet, and control condition of culture, so that microalgae is adsorbed by fungi pellet completely, and then using simple filtration side Method harvesting microalgae the process producing for bioenergy.
Background technology
Microalgae is that a class is nutritious, the high photosynthetic microorganism of photosynthetic efficiency.There is growth fast, oil content is high, culture is not The advantages such as occupation of land, are that the bio-fuel of preparing receiving much concern in the world is rich in co with fixing2The green cell factory of waste gas.This Outward, microalgae can be using the small organic molecule in sewage and inorganic nutrients, and ammonia nitrogen is the nitrogen source form of its most easily utilization, microalgae Can by photosynthesis purify high ammonia-nitrogen wastewater, need not additional organic carbon, less need the exposure in conventional waste water processing procedure Gas, significantly reduces water process energy consumption and cost.Microalgae can also accumulate the super long-chain unsaturated fatty acid of a large amount of needed by human body, such as flower Raw tetraenoic acid (ara), eicosapentaenoic acid (epa) and docosahexenoic acid (dha) etc. and various pigments, such as chlorophyll, shrimp Blue or green element, phycocyanin, carotenoid etc., these raw materials that can act as functional food additives and produce health product.
Microalgae has many advantages, and its commercial applications also obtains extensive concern, but the harvesting of microalgae always limit micro- The maximum bottleneck that algae is commercially produced.The microalgae balling-up flocculant biological harvesting worked in coordination with based on funguses that our seminars propose first Technology, this technology due to its have safe, to environment non-secondary pollution, high recovery rate, low energy consumption, low water content, can be continuous The advantages of harvesting, high biomass and lipid-producing, reusable edible medium matrix, and energy consumption and equipment investment and additive method Compare and significantly reduce it is considered to be most one of harvesting technique of future, but the greatest problem that this technology exists is collecting time Long (about 48 hours about), and there is species specificity and to balling-up key factor shortage systematic study for the funguses of harvesting And evaluation.Based on this, the present invention passes through to cultivate filamentous fungis balling-up, directly adds spheroid in algae solution to be harvested, and reaches fast The purpose of fast harvesting microalgae.The method is simple, and single mycelium pellet culture efficiency is high, and technology maturation is with low cost, and microalgae is received Time of obtaining short efficiency high, is a kind of microalgae bioflocculation collecting method of optimization.
Content of the invention
It is an object of the invention to provide a kind of quick harvesting method of microalgae of fungi pellet mediation.Can be become by culture Ball funguses, being forwarded to after culture balling-up in the micro algae culturing liquid after enrichment, making fungi pellet that microalgae is adsorbed, thus opening Send out new microalgae harvesting method simply efficient, with low cost a kind of.
The method of described fungi pellet harvesting microalgae is to cultivate microalgae cell in bioreactor middle-high density, then Import fungi pellet in the different times of cell growth and control culture to obtain the mycelium pellet of absorption microalgae, and then using simple Filter method harvesting microalgae and for bioenergy produce process.
A kind of quick harvesting method of microalgae of described fungi pellet mediation follows the steps below: (1) microalgae Switching；(2) High Density Cultivation of microalgae；(3) culture of energy balling-up fungal spore；(4) the formation culture of mycelium pellet；(5) by bacterium Pompon is transferred in micro algae culturing liquid；(6) mycelium pellet is cultivated to microalgae adsorption process；(7) harvest absorption microalgae with filter method Mycelium pellet.
Described microalgae includes but is not limited to Chlorella (chlorella sp.), barrel mast Trentepohlia (cylindrotheca Sp.), diatom (diatom), rhombus algae (nitzschia sp.), split kettle algae (schizochytrium sp.), Dunaliella (dunaliella), scenedesmus (scenedesmus sp.), Nannochloropsis oceanica (nannochloris sp.), Chlamydomonas (chlamydomonas sp.), flat algae (tetraselmis sp.), empty ball Trentepohlia (eudorina sp.) etc..
Described micro-algae culture medium includes but is not limited to bg-11 culture medium, f/2 culture medium, walne culture medium, tap culture Base and other any micro-algae culture mediums that may be modified.
The fungal bacterial strain of described energy balling-up includes, but are not limited to Rhizopus oryzae (rhizopus oryzae), variable color Auricularia (anthracophyllum discolor), oyster cap fungus (pleurolus ostreatus), Morchella esculenta (L.) Perss (morchella. Sp), Penicllium chrysogenum (penicillum chrysogenum), Agaricus bisporuss (agaricus bisporus), mucor (mucorcircillenous), Mortierella isabellina (mortierella isabellina), Aspergillus phoenicis (aspergillus Phonecis), trichoderma reesei (trichoderma reesei), the flat lead fungi (phanerochaete of yellow spore ) and Pleurotus eryngii (pleurotus eryngii), Lentinus Edodess (letinous edodes), Pleurotus ostreatus chrysosporium (pleuroutus ostreatus), agrocyb eaegerita (agrocybe aegerita), JIGU (pleurotus cornucopiae), Pleurotus geesteranus (pleurotus pulmonarius), Flammulina velutiper (Fr.) Sing (flammulina velutipes), Volvariella volvacea (Bull.Ex Franch.) Singer． (volvariella Volvacea), Agaricus bisporuss (agaricus bisporus), Hericium erinaceus (Bull. Ex Fr.) Pers. (hericium erinaceus) and crab flavour mushroom Edible fungi such as (hypsizygus marmoreus).
Described fungi culture medium includes but is not limited to potato dextrose agar (pda) or the training of potato glucose liquid Foster base (pdb) or mmn culture medium.
In the present invention, the culture of described helotism body contains autotrophy culture or the method for Heterotrophic culture.Switching mycelia Bulb diameter is the preferred 3-5mm of 0.1-50mm；The mycelium pellet quantity of switching is 5-100/100ml algae solution；Ph scope is 0.1-10； Temperature range is 4-45 DEG C；Rotating speed 10-300 rpm；Heterotrophic culture base organic carbon source concentration initial reduction sugar concentration range be 0.01-200g·l-1.
The techniqueflow that present invention is related to and method and step are described in detail as follows.
(1) switching of microalgae
Take out frozen microalgae algae strain from -70 degree refrigerators and with inoculating loop scraping a little to solid slope flat board illumination cultivation.
Described autotrophy condition of culture is as follows: temperature control is in the range of 4-45 DEG C；Illumination cultivation nitrogen source such as glycine, The initial concentrations such as yeast extract are between 1-15g l-1, it is passed through air or air and co2Mixed gas, ventilation 50- 300l/h；co2Concentration 0.9-3%;10-200 μm of ol m is adopted in incubation-2s-1Daylight illumination, ph value control 5-9 it Between, depending on total incubation time visual cell growing state, it is typically in the range of 50-400 hour, preferably 120-200 hour.
Described Heterotrophic culture condition is as follows: organic carbon source such as concentration of glucose 0.01-200g l-1；It is passed through air, lead to Tolerance 100-400l/h, preferably 150-250l/h；1-200 μm of ol m is adopted in incubation-2s-1Sun exposure, ph value Control between 1-10, depending on total incubation time visual cell growing state, be typically in the range of 72-400 hour, preferably 100-150 is little When.
(2) High Density Cultivation of microalgae
Plate culture is seeded to bioreactor culture, in the culture of Heterotrophic culture base middle-high density；Biological reaction apparatus bag Include shaking flask, ventilation bottle, bioreactor, fermentation tank and open culturing pond.In the culture of above-mentioned suitable condition, until cell log Trophophase, cell density reaches 106-1010More than.
Described autotrophy condition of culture is as follows: temperature control is in the range of 4-45 DEG C；Illumination cultivation nitrogen source such as glycine, The initial concentrations such as yeast extract are between 1-15g l-1, it is passed through the mixed gas of air or air and co2, ventilation 50- 300l/h, preferably 80-120l/h；co2Concentration 0.9-3%;1-200 μm of ol m is adopted in incubation-2s-1Daylight illumination, ph Value controls between 5-9, depending on total incubation time visual cell growing state, is typically in the range of 50-400 hour, preferably 120-200 is little When.
Described Heterotrophic culture condition is as follows: organic carbon source such as concentration of glucose 0.01-200g l-1, preferably 20 g l-1；It is passed through air, ventilation 100-400l/h, preferably 150-250l/h；5-40 μm of ol m is adopted in incubation-2s-1Day Light irradiation, ph value controls between 5-9, is preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 72- 400 hours, preferably 100-150 hour.
(3) Spore cultivation of energy balling-up funguses
Take out frozen fungal bacterial strain and with inoculating loop scraping a little to solid plate culture 4-7 days from -70 degree refrigerators, then use no Bacterium water repeatedly rinses and obtains fresh fungal spore.
(4) the formation culture of mycelium pellet
The Fresh spores that will obtain, are added in pdb culture medium, use 250ml shaking flask, cultivate in 28 DEG C of ± 5 DEG C of shaking tables, and shape Become bulb forms;, add 1 × 10 taking Aspergillus phoenicis (aspergillus. sp.) as a example2l-1To 1 × 1010l-1Fresh spore Son in pdb culture medium, preferably 1 × 104l-1Fungal spore concentration.For preventing from causing miscellaneous bacteria during funguses balling-up Pollution, all operations are all aseptically carried out.
(5) mycelium pellet is transferred in micro algae culturing liquid
The mycelium pellet that step (1) logarithmic growth latter stage microalgae culture and step (5) culture are obtained is transferred to the micro- of step (3) In algae Heterotrophic culture base, use 250ml shaking flask, cultivate in 28 DEG C of ± 5 DEG C of shaking tables, and form bulky grain helotism body；
(6) mycelium pellet is cultivated to microalgae adsorption process
Optimum culture condition, until the relayed mycelium pellet absorption of all microalgae.
(7) harvest the mycelium pellet of absorption microalgae with filter method, and produce for bioenergy
Above-mentioned autotrophy culture medium prescription is: k2hpo4·3h2O 0.04g/l, mgso4·7h2O 0.075g/l, cacl2·2h2o 0.036g/l, citric acid 0.006g/l, ferric ammonium citrate 0.006g/l, edta 0.001g/l, nano31.5g/l, na2co3 0.02g/l, a5 liquid microelement 1.5ml/l, wherein a5 liquid microelement form: h3bo32.86g/l, mncl2·4h2o 1.81g/l, znso4·7h2O 0.222g/l, namoo4·2h2O 0.39g/l, cuso4·5h2O 0.079g/l and cocl2·6h2o 0.05g/l；
Above-mentioned Heterotrophic culture based formulas are: in above-mentioned autotrophy culture medium, addition organic carbon source to initial reduction sugar concentration is 0.01-200g·l-1；Preferred scope is in 1-20 g l-1.
The present invention is by quoting fungi pellet in the micro-algae culture medium that will harvest, co-culturing with optimal conditions And all microalgae are adsorbed, thus reaching the purpose of harvesting microalgae, can harvest microalgae finally by simple filter method The mycelium pellet of absorption, and produce for bioenergy.
The beneficial effects of the present invention is: introduce the mechanism that fungi pellet adsorbs microalgae.This technique efficiently controls The cost that microalgae is collected, meets the requirement of microalgae biodiesel industrial applications, is economy, efficiently microalgae receipts processed The new way obtaining.
Brief description
Fig. 1 is mycelium pellet photo of the present invention；
Fig. 2 is state before mycelium pellet absorption algae solution of the present invention；
Fig. 3 is mycelium pellet of the present invention absorption algae solution state after 2.5 hours；
Fig. 4 is flocculation efficiency under difference concentration of glucose of the present invention；
In figure abscissa is concentration of glucose (g/l), and vertical coordinate is flocculation efficiency (%)
Fig. 5 is flocculation efficiency under different rotating speeds of the present invention；
In figure abscissa is rotating speed (rpm), and vertical coordinate is flocculation efficiency (%)
Fig. 6 is that the difference number mycelium pellet adsorption efficiencies that add of the present invention change；
In figure abscissa is mycelium pellet number (individual), and vertical coordinate is flocculation efficiency (%)；
Fig. 7 is adsorption efficiency under different temperatures of the present invention；
In figure abscissa temperature (DEG C), vertical coordinate is flocculation efficiency (%)；
Fig. 8 is adsorption efficiency under difference ph of the present invention；
In figure abscissa is ph, and vertical coordinate is flocculation efficiency (%).
Specific embodiment
The method of the invention comprises the following steps.
(1) switching of microalgae
Take out frozen microalgae algae strain from -70 degree refrigerators and with inoculating loop scraping a little to solid slope flat board illumination cultivation.
Described autotrophy condition of culture is as follows: temperature control, in the range of 20-45 DEG C, is optimal with 28 DEG C；Illumination is trained The foster nitrogen source such as initial concentration such as glycine, yeast extract is between 1-15g l-1, preferably 4 g l-1, it is passed through air or air With co2Mixed gas, ventilation 50-300l/h, preferably 80-120l/h；co2Concentration 0.9-3%.10- is adopted in incubation 200μmol·m-2s-1Daylight illumination, ph value controls between 5-9, is preferred with 7.0；Total incubation time visual cell growing state Depending on, it is typically in the range of 50-400 hour, preferably 120-200 hour.
Described Heterotrophic culture condition is as follows: organic carbon source such as concentration of glucose 0.01-200g l-1, preferably 20 g l-1；It is passed through air, ventilation 100-400l/h, preferably 150-250l/h；5-40 μm of ol m is adopted in incubation-2s-1Day Light irradiation, ph value controls between 5-9, is preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 72- 400 hours, preferably 100-150 hour.
(2) High Density Cultivation of microalgae.
Plate culture is seeded to bioreactor culture, in the culture of Heterotrophic culture base middle-high density；Biological respinse fills Put including shaking flask, ventilation bottle, bioreactor, fermentation tank and open culturing pond.In the culture of above-mentioned suitable condition, until cell Exponential phase, cell density reaches 106-1010More than.
Described autotrophy condition of culture is as follows: temperature control, in the range of 20-45 DEG C, is optimal with 28 DEG C；Illumination is trained The foster nitrogen source such as initial concentration such as glycine, yeast extract is between 1-15g l-1, preferably 4 g l-1, it is passed through air or air With the mixed gas of co2, ventilation 50-300l/h, preferably 80-120l/h；co2Concentration 0.9-3%.10- is adopted in incubation 200μmol·m-2s-1Daylight illumination, ph value controls between 5-9, is preferred with 7.0；Total incubation time visual cell growing state Depending on, it is typically in the range of 50-400 hour, preferably 120-200 hour.
Described Heterotrophic culture condition is as follows: organic carbon source such as concentration of glucose 0.01-200g l-1, preferably 20 g l-1；It is passed through air, ventilation 100-400l/h, preferably 150-250l/h；5-40 μm of ol m is adopted in incubation-2s-1Day Light irradiation, ph value controls between 5-9, is preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 72- 400 hours, preferably 100-150 hour.
(3) Spore cultivation of energy balling-up funguses.
From -70 degree refrigerators take out frozen fungal bacterial strains and with inoculating loop scraping a little to solid plate cultivate 4-7 days, then Repeatedly rinsed with sterilized water and obtain fresh fungal spore.
(5) the formation culture of mycelium pellet.
The Fresh spores that will obtain, are added in pdb culture medium, use 250ml shaking flask, cultivate in 28 DEG C of ± 5 DEG C of shaking tables, And form bulb forms., add 1 × 10 taking Aspergillus phoenicis (aspergillus. sp.) as a example2l-1To 1 × 1010l-1New Fresh spore in pdb culture medium, preferably 1 × 104l-1Fungal spore concentration.For preventing from causing during funguses balling-up Living contaminantses, all operations are all aseptically carried out.
(5) mycelium pellet is transferred in micro algae culturing liquid.
The mycelium pellet that step (1) logarithmic growth latter stage microalgae culture and step (5) culture are obtained is transferred to step (3) Heterotrophic microalgae culture medium in, use 250ml shaking flask, cultivate in 28 DEG C of ± 5 DEG C of shaking tables, and form bulky grain helotism body；
(6) mycelium pellet is cultivated to microalgae adsorption process.
Optimum culture condition, until the relayed mycelium pellet absorption of all microalgae.
(7) harvest the mycelium pellet of absorption microalgae with filter method, and produce for bioenergy.
Above-mentioned autotrophy culture medium prescription is: k2hpo4·3h2O 0.04g/l, mgso4·7h2O 0.075g/l, cacl2· 2h2O 0.036g/l, citric acid 0.006g/l, ferric ammonium citrate 0.006g/l, edta 0.001g/l, nano31.5g/l, na2co30.02g/l, a5 liquid microelement 1.5ml/l, wherein a5 liquid microelement form: h3bo32.86g/l, mncl2· 4h2O 1.81g/l, znso4·7h2O 0.222g/l, namoo4·2h2O 0.39g/l, cuso4·5h2O 0.079g/l, and cocl2·6h2o 0.05g/l；
Above-mentioned Heterotrophic culture based formulas are: in above-mentioned autotrophy culture medium, addition organic carbon source to initial reduction sugar concentration is 0.01-200g·l-1；Preferred scope is in 1-20 g l-1.
Embodiment
Oil-containing algae chlorella (cholorella vulgaris) is local to be screened, and its autotrophy culture medium prescription is as follows.
Above-mentioned autotrophy culture medium prescription is: k2hpo4·3h2O 0.04g/l, mgso4·7h2O 0.075g/l, cacl2· 2h2O 0.036g/l, citric acid 0.006g/l, ferric ammonium citrate 0.006g/l, edta 0.001g/l, nano31.5g/l, na2co30.02g/l and cocl2·6h2O 0.05g/l, a5 liquid microelement 1.5ml/l, wherein a5 liquid microelement group Become: h3bo32.86g/l, mncl2·4h2O 1.81g/l, znso4·7h2O 0.222g/l, namoo4·2h2O 0.39g/l, cuso4·5h2o 0.079g/l.
Heterotrophic culture based formulas ibid, and add organic carbon source to be 20 g l to initial reduction sugar concentration-1.
By the method described in step (1), the chlorella being grown on solid medium advantage individual plant group is connect Plant to shaking table culture in 250ml shaking flask, temperature control is at 20 DEG C ± 5 DEG C；After cell enters logarithmic growth latter stage, prepare switching Fungi pellet.
In -70 DEG C of refrigerators, inoculation Aspergillus phoenicis (aspergillus. sp.) spore activates in solid plate culture medium Fungal bacterial strain, then with sterilized water washing plate surface repeatedly, obtains every liter and contains 1 × 106l-1Fresh Aspergillus phoenicis (aspergillus. sp.) spore, in liquid medium within, shaking table culture generates oarse-grained Aspergillus phoenicis (aspergillus. sp.) bulb forms, culture gained mycelium pellet are forwarded in micro algae culturing liquid and adsorb fixing microalgae.
Fig. 1 to Fig. 3 is respectively fungi pellet, mycelium pellet absorption is front and adsorbs the photo completing, and chooses concentration of glucose 0th, 1,3,5g/l, adsorption efficiency is as shown in figure 4, concentration of glucose is that during 3g/l, adsorption effect is optimal；Right under the conditions of different rotating speeds Microalgae cell is adsorbed, and result shows that rotating speed is that during 100rpm, adsorption effect is optimal, such as Fig. 5；Also investigated not equality of temperature simultaneously Add the different impacts to adsorption effect for the number mycelium pellet, result shows, temperature is to add 30 mycelia when 38 DEG C under the conditions of degree Ball adsorption effect is preferably (Fig. 6, Fig. 7).Ph is very big on adsorption efficiency impact, and Fig. 8 is mycelium pellet adsorption effect under the conditions of different ph, It can be seen that ph is that when 4, adsorption effect is optimal.
Immobilized chlorella (cholorella vulgaris)-Aspergillus phoenicis are harvested by simple filter method The helotism spheroid of (aspergillus. sp.) simultaneously reclaims for biomass.

Claims (10)

1. a kind of quick harvesting method of microalgae of fungi pellet mediation, is characterized in that: comprise the following steps:
(1) described microalgae cultivates the latter stage of exponential phase in microalgae autotrophy culture medium;
(2) inoculated fungi spore activates fungal bacterial strain in solid plate culture medium, and produces a large amount of Fresh spores；
(3) add organic carbon source in the autotrophy culture medium of step (1), become Heterotrophic microalgae culture medium；
(4) with sterilized water rinsing step (2) fungus solids plating medium surface repeatedly, obtain every liter and contain 1 × 102To 1 × 1010l-1Fresh fungal spore liquid；
(5) by the fresh fungal spore liquid being obtained in fungi culture medium, use 250ml shaking flask, cultivate in shaking table, formed true Bacterium bulb forms；
(6) step (1) logarithmic growth latter stage microalgae culture and step (5) fungi pellet are transferred to the microalgae of step (3) Heterotrophic culture base in, use 250ml shaking flask, in shaking table cultivate, until culture fluid in microalgae adsorbed completely by mycelium pellet；
(7) harvest the mycelium pellet by microalgae absorption completely of step (6) by filter method, with harvesting microalgae.
2. a kind of fungi pellet mediation as claimed in claim 1 the quick harvesting method of microalgae it is characterised in that: described micro- Algae is Chlorella, barrel mast Trentepohlia, diatom, rhombus algae, split kettle algae, Dunaliella, scenedesmus, Nannochloropsis oceanica, Chlamydomonas, flat algae or Empty ball Trentepohlia.
3. a kind of fungi pellet mediation as claimed in claim 1 the quick harvesting method of microalgae it is characterised in that: described micro- Algae culture medium, including microalgae autotrophy culture medium and Heterotrophic microalgae culture medium, is bg-11 culture medium, f/2 culture medium, walne culture Base or tap culture medium.
4. a kind of fungi pellet mediation as described in claim 1 and 3 the quick harvesting method of microalgae it is characterised in that: institute Stating microalgae autotrophy culture medium prescription is k2hpo4·3h2o 0.04g/l、mgso4·7h2o 0.075g/l、 cacl2·2h2o 0.036g/l, citric acid 0.006g/l, ferric ammonium citrate 0.006g/l, edta 0.001g/l, nano31.5g/l、na2co3 0.02g/l, a5 liquid microelement 1.5ml/l and cocl2·6h2O 0.05g/l, wherein a5 liquid microelement consists of: h3bo32.86g/l、mncl2·4h2o1.81g/l、znso4·7h2o 0.222g/l、namoo4·2h2o 0.39g/l、 cuso4·5h2o 0.079g/l.
5. a kind of fungi pellet mediation as claimed in claim 1 the quick harvesting method of microalgae it is characterised in that: described energy The fungal bacterial strain of balling-up is Rhizopus oryzae, variable color Auricularia, oyster cap fungus, Morchella esculenta (L.) Perss, Penicllium chrysogenum, Agaricus bisporuss, mucor, deep yellow Mortierella, Aspergillus phoenicis, trichoderma reesei or the yellow flat lead fungi of spore.
6. a kind of fungi pellet mediation as claimed in claim 1 the quick harvesting method of microalgae it is characterised in that: described energy The fungal bacterial strain of balling-up include Pleurotus eryngii in edible fungi, Lentinus Edodess, Pleurotus ostreatus, agrocyb eaegerita, JIGU, Pleurotus geesteranus, Flammulina velutiper (Fr.) Sing, Volvariella volvacea (Bull.Ex Franch.) Singer．, Agaricus bisporuss, Hericium erinaceus (Bull. Ex Fr.) Pers. or crab flavour mushroom.
7. a kind of fungi pellet mediation as claimed in claim 1 the quick harvesting method of microalgae it is characterised in that: described Fungi culture medium is that potato dextrose agar is pda or potato dextrose broth is pdb or mmn culture medium.
8. a kind of fungi pellet mediation as claimed in claim 1 the quick harvesting method of microalgae it is characterised in that: described bacterium The culture of algae homobium comprises autotrophy culture or the method for Heterotrophic culture；The described switching a diameter of 0.1-50mm of mycelium pellet；Switching Mycelium pellet quantity be 5-100/100ml；Described culture medium ph is 0.1-10, and cultivation temperature is 4-45 DEG C；Described shaking table turns Fast 10-300 rpm；Described Heterotrophic culture base organic carbon source concentration initial reduction sugar concentration is 0.01-200g l-1.
9. a kind of fungi pellet mediation as claimed in claim 8 the quick harvesting method of microalgae it is characterised in that: described from Foster condition of culture is as follows: temperature control 4-45 DEG C；Illumination cultivation nitrogen source glycine or yeast extract initial concentration are between 1- 15g·l-1, it is passed through air or air and co2Mixed gas, ventilation 50-300l/h, co2Concentration 0.9-3%, incubation Middle 10-200 μm of ol m of employing-2s-1Daylight illumination, ph value 5-9, total incubation time 50-400 hour.
10. a kind of fungi pellet mediation as claimed in claim 8 the quick harvesting method of microalgae it is characterised in that: described Heterotrophic culture condition is as follows: organic carbon source concentration of glucose 0.01-200g l-1, it is passed through air, ventilation 100-400l/h, training 1-200 μm of ol m is adopted during supporting-2s-1Sun exposure, ph value 0.1-10, total incubation time 72-400 hour.
CN201610827066.7A 2016-09-18 2016-09-18 Hyphae pellet mediated fast microalga harvesting method Pending CN106350455A (en)

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CN107723242A (en) * 2017-08-28 2018-02-23 北京联合大学 A kind of method for comprehensively utilizing rubbish from cooking zymotic fluid culture microalgae
CN109370926A (en) * 2018-12-25 2019-02-22 黑龙江八农垦大学 A method of pot algae compound is split by co-culturing production oyster mushroom-using starch
CN109439535A (en) * 2018-12-04 2019-03-08 南昌大学 A kind of micro algae culturing device
CN110846234A (en) * 2019-12-23 2020-02-28 上海市农业科学院 Pleurotus cornucopiae culture medium
CN112553084A (en) * 2020-11-05 2021-03-26 扬州大学 Preparation method of composite microorganism functional feed
CN114395481A (en) * 2022-01-25 2022-04-26 成都大学 Method for harvesting microalgae by using fungal floating biological mat
CN115044484A (en) * 2022-06-16 2022-09-13 中南大学 Method for harvesting microalgae

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Cited By (12)

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Publication number Priority date Publication date Assignee Title
CN107354098A (en) * 2017-08-08 2017-11-17 江苏大学 A kind of common harvesting method of microalgae cultivated using filamentous algae when similar
CN107723242A (en) * 2017-08-28 2018-02-23 北京联合大学 A kind of method for comprehensively utilizing rubbish from cooking zymotic fluid culture microalgae
CN107723242B (en) * 2017-08-28 2021-05-25 北京联合大学 Method for culturing microalgae by comprehensively utilizing kitchen garbage fermentation liquor
CN109439535A (en) * 2018-12-04 2019-03-08 南昌大学 A kind of micro algae culturing device
CN109439535B (en) * 2018-12-04 2023-09-26 南昌大学 Microalgae culture device
CN109370926A (en) * 2018-12-25 2019-02-22 黑龙江八农垦大学 A method of pot algae compound is split by co-culturing production oyster mushroom-using starch
CN109370926B (en) * 2018-12-25 2022-03-01 黑龙江八一农垦大学 Method for producing oyster mushroom-schizochytrium limacinum compound by co-culture by using starch
CN110846234A (en) * 2019-12-23 2020-02-28 上海市农业科学院 Pleurotus cornucopiae culture medium
CN112553084A (en) * 2020-11-05 2021-03-26 扬州大学 Preparation method of composite microorganism functional feed
CN114395481A (en) * 2022-01-25 2022-04-26 成都大学 Method for harvesting microalgae by using fungal floating biological mat
CN114395481B (en) * 2022-01-25 2024-01-09 成都大学 Method for harvesting microalgae by using fungal floating biological pad
CN115044484A (en) * 2022-06-16 2022-09-13 中南大学 Method for harvesting microalgae

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Application publication date: 20170125