CN115044484A - Method for harvesting microalgae - Google Patents

Method for harvesting microalgae Download PDF

Info

Publication number
CN115044484A
CN115044484A CN202210736457.3A CN202210736457A CN115044484A CN 115044484 A CN115044484 A CN 115044484A CN 202210736457 A CN202210736457 A CN 202210736457A CN 115044484 A CN115044484 A CN 115044484A
Authority
CN
China
Prior art keywords
microalgae
culturing
fungus
harvesting
mycelium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210736457.3A
Other languages
Chinese (zh)
Inventor
申丽
王俊俊
田庆华
曾伟民
吴学玲
李交昆
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Central South University
Original Assignee
Central South University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Central South University filed Critical Central South University
Priority to CN202210736457.3A priority Critical patent/CN115044484A/en
Publication of CN115044484A publication Critical patent/CN115044484A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/68—Aspergillus fumigatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/80—Penicillium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method for harvesting microalgae, which comprises the steps of inoculating 5-8% of microalgae to a sterilized BG-11 culture medium, and culturing in a light incubator to logarithmic phase to obtain microalgae culture solution for later use; culturing fungi for 48h to form mycelium pellets, separating the mycelium pellets, washing with sterilized normal saline, adding into microalgae culture solution, shake culturing in a shaking table, and regularly observing the fixed harvesting effect of microalgae. According to the invention, the microalgae fixed recovery is relatively researched through the industrial flocculant and the fungal mycelium pellet, a mycelium pellet symbiotic system (a mycelium pellet symbiotic system) formed by filamentous fungi and microalgae is a novel immobilization mode, the filamentous fungi capable of forming the pellet is used as a biomass carrier, and the microalgae is fixed on the surface and inside of the mycelium pellet in the ways of adsorption, embedding and the like, so that the microalgae can be efficiently recovered at low cost.

Description

Method for harvesting microalgae
Technical Field
The invention relates to a method for harvesting microalgae, in particular to a method for harvesting microalgae by using biomass carrier mycelium pellets.
Background
The biological adsorption is an adsorption process based on various biochemical action mechanisms such as ion exchange, surface complexation and intracellular precipitation by taking a microbial material as an adsorbent, has the advantages of simple operation, low operation cost, no secondary pollution, heavy metal recovery, water body recycling after treatment and the like, and is a novel heavy metal wastewater treatment technology. In recent years, researches on adsorption theory and practical application of heavy metal wastewater adsorption treatment by using biological adsorbents such as bacteria, fungi and algae and other biomass adsorbents such as chitin and straw are remarkably increased. Among them, microalgae have high heavy metal enrichment capacity and excellent regeneration characteristics, and show great advantages in the aspects of treating heavy metal pollution and recovering precious metals.
Microalgae are microorganisms which are widely distributed in nature, commonly exist in aquatic environment, contain chlorophyll A and can perform photoautotrophy, the outer cell wall is of a porous reticular structure, and is rich in Extracellular Polymeric Substances (EPS) taking polysaccharides, proteins, nucleic acids and lipids as key components, the EPS has abundant charges and can provide various adsorption groups (such as carboxyl, hydroxyl, carbonyl, phosphodiester and other groups), and the microalgae are ideal materials for combining charged heavy metal ions, metalloids and organic pollutants. Pradhan and the like can efficiently remove Cr from wastewater by using Scenedesmus sp 6+ The highest removal rate can reach 92.89%; husien et al showed that brown algae Sargassum wightii is responsible for Pb 2+ And Cd 2+ The adsorption rate of the compound is 80 percent, and the green alga Caulerpa racemosa has Cd pair 2+ And Cr 6+ The adsorption rate of (2) was 85%. Therefore, the microalgae can be used as an efficient biological adsorbent to treat various heavy metal pollutions.
Although microalgae have great potential and unique advantages in heavy metal wastewater treatment, the problem of high cost of microalgae recovery is a bottleneck limiting their industrial application. The microalgae suspension has tiny cell individuals, low density and negative charge on the cell surface, forms a natural and stable dispersion system in the culture solution, and is difficult to collect and recycle after adsorbing heavy metal ions. At present, different methods are used by researchers for harvesting microalgae, and the species of microalgae to be harvested are different according to the requirements. At present, microalgae recovery mainly comprises technologies such as centrifugation, filtration, flocculation, gravity sedimentation, flotation and the like, but the methods generally have the problems of low recovery efficiency, high energy consumption, high operation cost and the like, the recovery cost usually accounts for about 20-30% of the total production cost, even reaches 50% in some cases, and the development of a simple, efficient and low-cost microalgae recovery method is urgently needed.
Disclosure of Invention
The invention aims to provide a microalgae harvesting method which can flocculate and settle microalgae and has high efficiency and low cost. The invention is realized by the following technical scheme:
the invention relates to a method for harvesting microalgae, which comprises the following steps:
(1) inoculating 5-8% microalgae to the sterilized BG-11 culture medium, and culturing in a light incubator to logarithmic phase to obtain microalgae culture solution;
(2) culturing fungus for 48h to form mycelium pellet, separating the mycelium pellet, washing with sterilized normal saline, adding into microalgae culture solution, shake culturing in shaking table, and observing the fixing effect of the mycelium pellet on microalgae at regular time.
Further, the conditions of the illumination incubator in the step (1) are that the illumination intensity is 2000Lux, the temperature is 25-30 ℃, the pH value is 7.0-7.1, and the illumination/darkness ratio is 12h:12 h.
Further, the log phase bacterial concentration of the step (1) is 10 8 one/mL.
Further, the fungus of step (2) is selected from the group consisting of:
fungus Aspergillus fumigatus No. 1;
fungus Penicillium glaucosum No. 2;
fungus Aspergillus fumigatus No. 3.
Further, the fungal sources include:
the fungus used in the experiment is from the water sample of the Toping Town Liuxi heavy metal wastewater
Separating and purifying fungi: the filamentous fungi are obtained by screening PDA plates containing heavy metals Cd (II) and Cr (VI). Firstly, respectively coating collected wastewater samples (200uL) on solid plates containing 5mg/L Cd (II) and Cr (VI). Culturing the plate in 30 deg.C constant temperature incubator for 5-7 days, after single fungus grows out, streaking onto fresh PDA plate containing heavy metal, repeating for at least 5 times, and finally obtaining single filamentous fungus. Selecting two liquid culture mediums, PDB culture medium and beef extract culture medium, respectively, from different fungi obtained by separation, performing balling test in a constant temperature shaking table under the conditions of rotation speed of 120r/min, temperature of 30 deg.C and initial spore inoculation amount of 10 4 And (4) comparing the balling condition of the filamentous fungi in different liquid culture media.
And secondly, selecting 3 separated fungi, preparing hyphae into a fungus suspension, transferring the fungus suspension to a liquid culture medium filled with 100mL of fungi, placing the fungus suspension in a constant-temperature shaking table for culturing for 48h, setting the temperature to be 30 ℃, rotating the speed to be 150r/min, observing the balling condition of different fungi in the culture medium, separating the mycelium pellets in a simple filtering mode, and washing the mycelium pellets with sterilized distilled water for 3 times to avoid the influence of the residual culture medium on the surface on subsequent results. The DNA was extracted and submitted to sequencing, the sequencing results were compared with the NCBI database by the BLAST Genbank and the sequencing results of the strains were used to construct phylogenetic trees using MEGA 6.06.
Thirdly, the strain identification is respectively as follows: aspergillus fumigatus, fungus No. 1, fungus No. 2, Penicillium glaucosum, fungus No. 3.
Further, the shaking table in the step (2) is vibrated under the conditions that the temperature is 25-30 ℃ and the rotating speed is 180 rpm/min.
Further, the timing of the step (2) is 0.5-24 h.
The invention has the beneficial effects that:
the method is simple to operate, has low requirements on equipment, and effectively solves the problems of high energy consumption and high cost in the microalgae harvesting technology.
Drawings
FIG. 1 is a graph of the effect of polyacrylamide on microalgae recovery at various time points;
FIG. 2 is a graph of the effect of aluminum sulfate on microalgae recovery at different time points;
FIG. 3 is a graph showing the effect of agar on microalgae harvesting at different time points;
FIG. 4 is a graph of the effect of chitosan on microalgae recovery at different time points;
FIG. 5 is a graph of the effect of calcium hydroxide on microalgae recovery at various time points;
FIG. 6 is a graph of the effect of sodium hydroxide on microalgae recovery at various time points;
FIG. 7 is a graph of the effect of fungus No. 1 on microalgae recovery at different time points;
FIG. 8 is a graph of the effect of fungus No. 2 on microalgae recovery at various time points;
FIG. 9 is a graph of the effect of fungus No. 3 on microalgae recovery at various time points.
Detailed Description
The present invention will be described in detail with reference to specific examples. It should be noted that the following examples are only illustrative of the present invention, but the scope of the present invention is not limited to the following examples.
Example 1
A method of harvesting microalgae, comprising:
(1) inoculating 5% microalgae to sterilized BG-11 culture medium, and culturing at 28 deg.C and pH7.1 under illumination intensity of 2000Lux and illumination/dark ratio of 12h:12h until the concentration is 10 8 Per mL;
(2) culturing Aspergillus fumigatus No. 1 (Aspergillus fumigatus fungus) for 48h to form mycelium pellet, separating the mycelium pellet, washing with sterilized normal saline, adding into the microalgae culture solution of (1), and performing shake culture in a shaking table at 28 deg.C and 150rpm/min for 0.5-24 h.
Example 2
A method of harvesting microalgae, comprising:
(1) inoculating 6% microalgae to sterilized BG-11 culture medium, and culturing at illumination intensity of 2000Lux, temperature of 30 deg.C, pH of 7.0, and illumination/dark ratio of 12h:12h until the concentration is 10 8 Per mL;
(2) culturing Penicillium glaucosum fungus No. 2 for 48h to form mycelium pellets, separating the mycelium pellets, washing with sterilized normal saline, adding into the microalgae culture solution of (1), and performing shake culture in a shaking table at 30 deg.C and 180rpm/min for 0.5-24h to observe the fixing effect of the mycelium pellets on microalgae regularly.
Example 3
A method of harvesting microalgae, comprising:
(1) inoculating 8% microalgae to sterilized BG-11 culture medium, and culturing at illumination intensity of 2000Lux, temperature of 30 deg.C, pH of 7.1 and illumination/dark ratio of 12h:12h until the concentration is 10 8 Per mL;
(2) culturing Aspergillus fumigatus No. 3 (Aspergillus fumigatus fungus) for 48h to form mycelium pellet, separating the mycelium pellet, washing with sterilized normal saline, adding into the microalgae culture solution of (1), and performing shake culture in a shaking table at 30 deg.C and 160rpm/min for 0.5-24h to observe the fixing effect of the mycelium pellet on microalgae regularly.
Comparative example
A method of harvesting microalgae, comprising:
(1) inoculating 5% microalgae to sterilized BG-11 culture medium, and culturing at illumination intensity of 2000Lux, temperature of 30 deg.C, pH of 7.1, and illumination/dark ratio of 12h:12h until the concentration is 10 8 Per mL;
(2) 0.15g/100mL of polyacrylamide (comparative example 1 group), aluminum sulfate (comparative example 2 group), agar (comparative example 3 group), chitosan (comparative example 4 group), calcium hydroxide (comparative example 5 group), and sodium hydroxide (comparative example 6 group) were added to the microalgal culture solution, respectively, and the flocculation fixation effect was observed for 0.5 to 24 hours.
Test example 1
System effect measurement analysis
Statistical analysis was performed on the microalgae harvesting effects in examples 1-3 and comparative examples 1-6, and the specific results are shown in fig. 1 and table 1.
TABLE 1 statistical analysis of microalgae harvesting effects in examples 1-3 and comparative examples 1-6
Grouping 0.5 h 3h 12h 24h
Polyacrylamide (D1) 90.08％ 95.56％ 136.55％ 109.92％
Aluminum sulfate (D2) 4.58％ 1.45％ -3.13％ -3.61％
Agar (D3) 103.78％ 101.03％ 92.44％ 93.81％
Chitosan (D4) 102.56％ 100.77％ 76.98％ 80.56％
Calcium hydroxide (D5) 1.60％ 9.89％ -2.67％ -3.48％
Sodium hydroxide (D6) 12.77％ 2.55％ -5.47％ -5.11％
Fungus No. 1 (S1) 14.53％ 100.63％ 101.88％ 101.88％
Fungus No. 2 (S2) 14.06％ 35.78％ 101.09％ 100.31％
Fungus No. 3 (S3) 25.00％ 88.59％ 95.94％ 102.19％
As can be seen from the results shown in fig. 1 and table 1, after the microalgae are fixed for 24 hours by the 6 industrial flocculants shown in fig. 1-6, good flocculation effects are shown only by aluminum sulfate (comparative example 2, fig. 2), calcium hydroxide (comparative example 5, fig. 5), and sodium hydroxide (comparative example 6, fig. 6), but all of the three process flocculants can cause the microalgae liquid to turn yellow, which indicates that the microalgae are close to death to a great extent, and no obvious advantages are shown in the microalgae harvesting effect. After the fungi 1 (embodiment 1, fig. 7), 2 (embodiment 2, fig. 8) and 3 (embodiment 3, fig. 9) fix the microalgae for 24 hours, the color of the culture solution tends to be clear and transparent, and the mycelium pellet adsorbs a large amount of microalgae cells to turn into green, so that the fungi 1, 2 and 3 are adopted to harvest the microalgae, not only can a good fixed harvesting effect be achieved, but also the death of the microalgae caused by the traditional industrial flocculant can be avoided, and the harvesting quantity and quality of the microalgae are further improved.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.

Claims (6)

1. A method of harvesting microalgae, comprising:
(1) inoculating 5-8% microalgae to the sterilized BG-11 culture medium, and culturing in a light incubator to logarithmic phase to obtain microalgae culture solution;
(2) culturing fungi for 48h to form mycelium pellets, separating the mycelium pellets, washing with sterilized normal saline, adding into microalgae culture solution, shake culturing in a shaking table, and regularly observing the fixed harvesting effect of microalgae.
2. The recovery method of claim 1, wherein:
the condition of the illumination incubator in the step (1) is that the illumination intensity is 2000Lux, the temperature is 25-30 ℃, the pH is 7.0-7.1, and the illumination/darkness ratio is 12h:12 h.
3. The recovery method of claim 1, wherein:
the logarithmic phase bacterial concentration of the step (1) is 10 8 one/mL.
4. The recovery method of claim 1, wherein:
the fungus of step (2) is selected from:
fungus Aspergillus fumigatus No. 1;
fungus Penicillium glaucosum No. 2;
fungus Aspergillus fumigatus No. 3.
5. The recovery method of claim 1, wherein:
the shaking table in the step (2) is vibrated at the temperature of 25-30 ℃ and the rotation speed of 150-.
6. The recovery method of claim 1, wherein:
and (3) regularly observing the fixed harvesting effect of the microalgae for 0.5-24 h.
CN202210736457.3A 2022-06-16 2022-06-16 Method for harvesting microalgae Pending CN115044484A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210736457.3A CN115044484A (en) 2022-06-16 2022-06-16 Method for harvesting microalgae

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210736457.3A CN115044484A (en) 2022-06-16 2022-06-16 Method for harvesting microalgae

Publications (1)

Publication Number Publication Date
CN115044484A true CN115044484A (en) 2022-09-13

Family

ID=83162875

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210736457.3A Pending CN115044484A (en) 2022-06-16 2022-06-16 Method for harvesting microalgae

Country Status (1)

Country Link
CN (1) CN115044484A (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120282651A1 (en) * 2011-05-02 2012-11-08 Renewuel Llc System and Method of Co-Cultivating Microalgae with Fungus
WO2013055887A1 (en) * 2011-10-14 2013-04-18 Regents Of The University Of Minnesota Microalgae culture and harvest
CN105602858A (en) * 2016-01-11 2016-05-25 南昌大学 Preparation method for edible fungus/selenium-rich microalgae complex
CN106350455A (en) * 2016-09-18 2017-01-25 南昌大学 Hyphae pellet mediated fast microalga harvesting method
CN112898374A (en) * 2021-02-05 2021-06-04 中南大学 Method for extracting extracellular polymer of bacterial-algae symbiotic system
CN114395481A (en) * 2022-01-25 2022-04-26 成都大学 Method for harvesting microalgae by using fungal floating biological mat

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120282651A1 (en) * 2011-05-02 2012-11-08 Renewuel Llc System and Method of Co-Cultivating Microalgae with Fungus
WO2013055887A1 (en) * 2011-10-14 2013-04-18 Regents Of The University Of Minnesota Microalgae culture and harvest
CN105602858A (en) * 2016-01-11 2016-05-25 南昌大学 Preparation method for edible fungus/selenium-rich microalgae complex
CN106350455A (en) * 2016-09-18 2017-01-25 南昌大学 Hyphae pellet mediated fast microalga harvesting method
CN112898374A (en) * 2021-02-05 2021-06-04 中南大学 Method for extracting extracellular polymer of bacterial-algae symbiotic system
CN114395481A (en) * 2022-01-25 2022-04-26 成都大学 Method for harvesting microalgae by using fungal floating biological mat

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HIRSTINA BODIN等: "Effects of biopellets composed of microalgae and fungi on cadmium present at environmentally relevant levels in water", INT J PHYTOREMEDIATION *
叶晨松: "基于丝状真菌的微藻收获技术研究及废水处理初探", 中国优秀硕士学位论文全文数据库 工程科技I辑 *

Similar Documents

Publication Publication Date Title
CN104673710B (en) Rhodococcus strain and its application
CN108587915B (en) Chlorella W5 capable of removing heavy metals in water with high heavy metal content and application thereof
CN108546649B (en) Chlorella W2 capable of removing heavy metals in water with high heavy metal content and application thereof
Li et al. Response of fungi-microalgae pellets to copper regulation in the removal of sulfonamides and release of dissolved organic matters
CN109554300B (en) Method for harvesting oil-producing microalgae in urban sewage culture system by using self-flocculating microalgae
CN112940961B (en) Microbial flocculant and preparation method and application thereof
CN109576159B (en) Chlorella W4 capable of removing heavy metals in water with high heavy metal content and application thereof
Zhang et al. Ammonia-nitrogen and orthophosphate removal by immobilized Chlorella sp. isolated from municipal wastewater for potential use in tertiary treatment
CN106047768A (en) Thauera bacterial strain and application thereof
Wang et al. Optimization of microalgal bead preparation with Scenedesmus obliquus for both nutrient removal and lipid production
CN109576160B (en) Chlorella W3 capable of removing heavy metals in water with high heavy metal content and application thereof
CN110656066A (en) Acinetobacter strain for shortcut nitrification and denitrification and application thereof
CN105567575A (en) New Purpureocillium sp strain F1 and application thereof
CN103833144A (en) Method for removing heavy metal ions in water by using fermentation broth of bacterium producing flocculant
CN112961790A (en) Heterotrophic nitrifying bacteria resistant to high-salt environment and application thereof
CN109576161B (en) Chlorella W1 capable of removing heavy metals in water with high heavy metal content and application thereof
CN115044484A (en) Method for harvesting microalgae
CN108546650B (en) Chlorella W6 capable of quickly removing heavy metals in water with high heavy metal content and application thereof
CN102994394A (en) Fungal strain LP-18-3 and application of fungal strain LP-18-3 in lead-containing water body treatment
CN107227260B (en) Biological adsorbent for adsorbing thallium ions and preparation method and application thereof
CN113249276B (en) Bacillus cereus and application thereof
CN116515665A (en) Pseudomonas capable of degrading microcystin, immobilized microbial agent and application
CN111378592A (en) Bacillus licheniformis and method for treating malodorous organic wastewater by using same to purify water
CN114437940A (en) Fungus flocculant, preparation method and application of fungus flocculant
CN108085350B (en) Method for preparing flocculant by using fast-growing bacillus strain

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination