CN100348514C - Deep-processing method of using starch watewater - Google Patents
Deep-processing method of using starch watewater Download PDFInfo
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- CN100348514C CN100348514C CNB2005100114982A CN200510011498A CN100348514C CN 100348514 C CN100348514 C CN 100348514C CN B2005100114982 A CNB2005100114982 A CN B2005100114982A CN 200510011498 A CN200510011498 A CN 200510011498A CN 100348514 C CN100348514 C CN 100348514C
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Abstract
The present invention discloses a method for producing microbial polyunsaturated fatty acid by making use of starch to process waste water. The method makes use of large amount of waste water generated in the starch processing process to produce the microbial polyunsaturated fatty acid. Starch is taken as a basic culture medium for processing the waste water, part of nutrition is added, and metallic ions are adjusted. After the waste water is sterilized, the waste water is supplied to be sieved, and microbial polyunsaturated fatty acid high yield bacterial strain is obtained. After the waste water is fermented by adopting an optimized fermentation technology, mycelium bodies are filtered, microbial grease with an obvious function is extracted and obtained, effluent water is controlled under a certain COD loading, and the present invention is recycled in a starch processing process. The waste water generated in the starch processing process by implementing the method of the present invention has resource utilization, the science level of a starch processing industry can be lifted effectively, the clean production in the starch processing process is realized, and the present invention generates obvious economic and social benefits.
Description
Technical field
The present invention relates to utilize starch wastewater to carry out deep-processing method, specifically, the invention provides a kind of starch wastewater that utilizes and produce the greasy method of microbial polyunsaturated fatty acid.
Background technology
Starch is widely used in a lot of industries, formed very big scale. according to of international food and the farming research report of nearest Dutch La Bo bank portion about world's Starch Production situation, world's starch ultimate production was about 4,900 ten thousand tons in 2003, wherein W-Gum is about 3,940 ten thousand tons, account for 80.4% of total amount, wheat starch accounts for 8.36% for about 4,100,000 tons, and tapioca (flour) accounts for 6.12% for about 3,000,000 tons, about 2,600,000 tons of potato starch accounts for 5.3%.
Need a large amount of water in the starch production process, 1 ton of starch of general production needs 10 tons more than the water, and this kind of starch waste water contains abundant organic matter such as starch mostly, micro-and relevant nutritional factor, COD is different because of different process, and high concentration starch water can reach about 80000.There is limited evidence currently of is administered, is utilized, and causes the environmental pollution and the wasting of resources.Along with the continuous enhancing of environmental protection requirement, waste water becomes the principal element of restriction starch industry development gradually.
The result that we cultivate the grease fungi to starch wastewater shows: starch wastewater can be used as the culture medium of grease fungi.Some fungies can be synthesized the PUFA grease in starch wastewater.Reduce waste water COD simultaneously, waste water is by resource type treating as a result.
Polyunsaturated fat is meant the polyunsaturated fatty acid that contains 20 and 20 above carbon atoms, is divided into ω-3 series as alpha linolenic acid (ALA, C according to the terminal double link position
18: 3n-3), timnodonic acid (EPA, C
20: 5n-3), docosahexenoic acid (DHA, C
22: 6n-3) and ω-6 series as linolic acid (LA, C
18: 2n-6), gamma-linolenic acid (GLA, C
18: 3n-6) arachidonic acid (AA, C
20: 4n-6) two big classes.
Present commercial ω one 3 polyunsaturated fatty acids mainly extract from fish oil, and complex process, cost height, poor stability also are subjected to the restriction of weather condition and resource.Except that the part animals and plants, polyunsaturated fatty acid is mainly derived from microorganism, especially ω one 3 polyunsaturated fatty acids.Many algae, fungi, bacterium have ω one 3 polyunsaturated fatty acids required serial desaturase and ligase enzyme, are the initial production persons of ω one 3 polyunsaturated fatty acids, and higher plant and animal then seldom contain ω one 3 polyunsaturated fatty acids.Microorganism has that adaptability is strong, growth and breeding is rapid, growth cycle is short, easily cultivate, be not subjected to characteristics such as places of origin of raw materials restriction, so utilizing the microorganisms producing polyunsaturated fatty acid is an important channel. at present, countries such as Japan, the Britain leavened prods such as gamma-linolenic acid, two high gamma-linolenic acid, arachidonic acid that successively come out, and EPA, DHA etc. still are in conceptual phase.
The emphasis that the research of microbial fermentation production polyunsaturated fatty acid is paid close attention to remains seeks superior strain and research zymotechnique.The production fermentation period is long, and producing in the special culture media needs high quality, high-load quick-acting carbon sources such as limiting factors such as glucose, maltose, has hindered microbial fermentation and has produced the PUFAs industrialization process.Therefore utilize low-cost resource necessary as fermented substrate.Utilize microbial technique recycling treatment starch wastewater then to can be to form the starch cleaner production and the recycling economy industry lays the foundation.
Summary of the invention
The object of the present invention is to provide a kind of starch processing waste water that utilizes to carry out the greasy microorganism deep process technology of microbial polyunsaturated fatty acid.Realize the ecology of starch secondary industry, solve the pollution and the resource utilization problem of waste water in the starch course of processing.
For achieving the above object, the inventive method can satisfy the needs of microorganisms producing at the relevant trace element that contains abundant starch nutritive ingredient and microorganism needs in the starch production wastewater.The advantage that obtains by screening is produced the polyunsaturated fatty acid grease microorganism and is utilized nutritive ingredient in the starch wastewater, and the synthesized micro-organism polyunsaturated fatty acid reduces the COD load in the waste water simultaneously, reaches the purpose of waste water as fermentation raw material and improvement.
Specifically, the invention provides a kind of starch wastewater that utilizes and carry out deep-processing method, it is characterized in that: in starch wastewater, add carbon source and promotor, after the sterilization, insert the polyunsaturated fatty acid grease superior strain and carry out fermentation culture, after the fermentation mycelium is filtered, and with effluent reuse in the starch course of processing.
Wherein said carbon source is a table sugar, and promotor is MgSO
4, MnSO
4, (NH
4)
2SO
4Preferably, in 1L waste water, add table sugar 10～30g, MgSO
42～3g, MnSO
41～2.5g, (NH
4)
2SO
40.8～1g.
The condition of described fermentation culture is: inoculum size (volume fraction v/v) 1～10%, plant 2d in age, 25～30 ℃ of temperature, incubation time 5～7 d, air flow (volume fraction v/v/h) 1: 1.2～1: 1.5, rotating speed 80～150r/min.
Preferably, liquid fermentation condition of the present invention is: inoculum size (volume fraction v/v) 1～5%,, temperature 25-28 ℃, incubation time 6～7 days, air flow 1: 1.2-1: 1.4, rotating speed 100～120r/min.
More preferably, fermentation condition of the present invention is: inoculum size (volume fraction v/v) 3%, incubation time 7 days, 26 ℃ of kind 2d temperature in age, air flow 1: 1.3, rotating speed 110r/min.
In the aforesaid method, described starch wastewater is cereal and piece root, tubers, the waste water that produces as all types starch such as corn starch wastewater, wheat starch waste water, potato starch waste water, sweet potato starch waste water, tapioca (flour) waste water, pueraria starch waste water, wild fruit starch processing back.
Polyunsaturated fatty acid grease microorganism of the present invention comprises, the bacterial strain of main product gamma-linolenic acid (GLA): cunninghamella echinulata (Cunninghamella echinulata) Fg etc.Single mao of spore of bacterial strain multiform of main product arachidonic acid (AA), timnodonic acid (EPA), docosahexenoic acid (DHA) (Monoblepharis polymorpha Comu) Ma and mutagenic fungi (Me), (Md) etc.
The hypha biomass that fermentation condition of the present invention produces is 16～20g/L, oil quantity is 7～10g/L, basal component is in the grease: nutmeg acid 0.27%～0.50%, palmitinic acid 12.65%～14.85%, physetoleic acid 0.78%～1.38%, stearic acid 2.89%～3.30%, oleic acid 33.4%～40.98%, linolic acid 16.47%～20.65%, eicosanoic acid 0.30%～1.67%, arachidonic acid 0.36%～0.78%, peanut diolefinic acid 0.20%～0.89%.
The main polyunsaturated fatty acid of imitating is because of the bacterial strain difference, and wherein the gamma-linolenic acid master imitates that bacterium (Fg) GLA content is 15.23%～25.45%, to imitate bacterium (Ma) AA content be 30.36%～42.45% to the arachidonic acid master; Dodecahexaene acid is main imitates that bacteria strain single mao of spore mutagenic fungi of multiform (Md) DHA content is 6.32%～9.65%, the timnodonic acid master is imitated single mao of spore mutagenic fungi of bacterium multiform mutagenic fungi (Me) EPA content 5.76%～9.90%.
Advantage of the present invention is:
(1) effectively solves pollution and the waste that starch wastewater produces, waste water is carried out reuse after the recycling treatment, for starch fabrication cycles economic industry is established technical foundation.
(2) produce polyunsaturated fatty acid with starch wastewater as fermentation raw material, because of utilizing waste water.The expense that not only can economize in raw materials, and save waste water treatment expense and water expense, help starch cleaner production and recycling economy.
(3) technology is controlled strong, is difficult for imitateing.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.
Embodiment 1
(1) strain preparation:
Polyunsaturated fatty acid grease high-yield strains preparation: with refrigerator preservation inclined-plane bacterial strain cunninghamella echinulata (Fg) (Cunninghamella echinulata), single mao of spore of multiform (Monoblepharis polymorpha Comu) (Ma) and mutagenic fungi (Me) (Md) insert respectively on PDA (solid potato culture medium) inclined-plane and activate, cultivate 7d, get mycelia and insert the 250ml that fills 100ml PDY (potato liquid) substratum and shake and carry out liquid culture in the bottle.Rotating speed 120～150r/min cultivates after 2 days down for 25-28 ℃, gets inoculum size (volume fraction v/v) 5～10%, and same liquid culture condition switching once obtains the liquid shaking bottle kind, and the seed preparation is carried out according to the reactor situation in the back.
(2) liquid fermenting production
Add table sugar with pressing 20g/L in the yam starch waste water, press MgSO
42g/l, MnSO
42.5g/l, (NH
4)
2SO
40.8g/l ratio adds promotor, form fermention medium, add polyunsaturated fat high yield bacterium cunninghamella echinulata (Fg) seed liquor for preparing by 3% (v/v), at 25 ℃ of temperature, air flow 1: 1.3, rotating speed 100r/min condition bottom fermentation 5 days, hypha biomass is 16.2g/L, and oil quantity is 7.2g/L, nutmeg acid 0.27% in the grease, palmitinic acid 12.65%, physetoleic acid 1.38%, stearic acid 2.89%, oleic acid 30.4%, linolic acid 20.47%, gamma-linolenic acid 18.67%, eicosanoic acid 1.67%, arachidonic acid 0.78%, peanut diolefinic acid 0.20%, EPA 0.6
Embodiment 2
Add table sugar with pressing 20g/L in the sweet potato starch waste water, press MgSO
43g/l, MnSO
41g/l, (NH
4)
2SO
4The 1g/l ratio adds promotor, forms fermention medium, adds single mao of spore of polyunsaturated fat high yield bacterium multiform (Ma) seed liquor of embodiment 1 preparation by 5% (v/v), 25 ℃ of temperature, air flow 1: 1.5, rotating speed 120r/min condition bottom fermentation 7 days, hypha biomass is 19.8g/L, oil quantity is 7.9g/L, nutmeg acid 0.50% in the grease, palmitinic acid 15.85%, physetoleic acid 1.38%, stearic acid 3.30%, oleic acid 10.56%, linolic acid 12.47%, gamma-linolenic acid 9.80%, eicosanoic acid 0.30%, arachidonic acid 0.72%, peanut diolefinic acid 0.89%, arachidonic acid 35.23%, EPA 0.80%, and DHA 1.98%.
Embodiment 3
Add table sugar with pressing 10g/L in the corn starch wastewater, press MgSO
42g/l, MnSO
42.5g/l, (NH
4)
2SO
4The 1g/l ratio adds promotor, form fermention medium, add polyunsaturated fat high yield bacterium single mao of spore mutagenic fungi of multiform (Me) seed liquor that embodiment 1 prepares by 3% (v/v), 25 ℃ of temperature, air flow 1: 1.5, rotating speed 80r/min condition bottom fermentation 7 days, hypha biomass is 18.5g/L, oil quantity is 8.5g/L, nutmeg acid 0.27% in the grease, palmitinic acid 14.85%, physetoleic acid 1.38%, stearic acid 3.30%, oleic acid 20.98%, linolic acid 12.47%, gamma-linolenic acid 9.80%, eicosanoic acid 0.30%, arachidonic acid 0.78%, peanut diolefinic acid 0.20, EPA 7.60%, and DHA 2.08%.
Embodiment 4
Add table sugar with pressing 30g/L in the wheat starch waste water, press MgSO
42g/l, MnSO
42.5g/l, (NH
4)
2SO
4The 1g/l ratio adds promotor, form fermention medium, add polyunsaturated fat high yield bacterium single mao of spore mutagenic fungi of multiform (Md) seed liquor that embodiment 1 prepares by 10% (v/v), 25 ℃ of temperature, air flow 1: 1.5, rotating speed 80r/min condition bottom fermentation 7 days, hypha biomass is 20g/L, oil quantity is 9.5g/L, nutmeg acid 0.27% in the grease, palmitinic acid 13.85%, physetoleic acid 2.38%, stearic acid 3.80%, oleic acid 23.98%, linolic acid 12.47%, gamma-linolenic acid 11.80%, eicosanoic acid 0.38%, arachidonic acid 0.82%, peanut diolefinic acid 0.25, EPA 2.60%, and DHA 8.08%.
Claims (7)
1, a kind of starch wastewater that utilizes carries out deep-processing method, it is characterized in that: add carbon source and promotor in starch wastewater, after the sterilization, insert product polyunsaturated fatty acid grease bacterial strain and carry out fermentation culture, after the fermentation mycelium is filtered, and with effluent reuse in the starch course of processing
Wherein, carbon source is a table sugar;
Wherein, promotor is selected from MgSO
4, MnSO
4, (NH
4)
2SO
4In one or more;
Wherein, the condition of fermentation culture is: inoculum size 1～10%, plant 2～3d in age, 25～30 ℃ of temperature, incubation time 5～7d, air flow 1: 1.2～1: 1.5, rotating speed 80～150r/min.
2, the method for claim 1, wherein the condition of described fermentation culture is: inoculum size 1～5%, and, 25～28 ℃ of temperature, incubation time 6～7d, air flow 1: 1.2～1: 1.4, rotating speed 100～120r/min.
3, the method for claim 1, wherein the condition of described fermentation culture is: inoculum size 3%, incubation time 7 days is planted 2d in age, 26 ℃ of temperature, air flow 1: 1.3, rotating speed 110r/min.
4, as each described method of claim 1～3, wherein said product polyunsaturated fatty acid grease bacterial strain is cunninghamella echinulata (Cunninghamella echinulata) or single mao of spore of multiform (Monoblepharis polymorpha Cornu).
5, as each described method of claim 1～3, it is characterized in that, in 1L waste water, add table sugar 10～30g, MgSO
42～3g, MnSO
41～2.5g, (NH
4)
2SO
40.8～1g.
As each described method of claim 1～3, it is characterized in that 6, described starch wastewater is corn starch wastewater, wheat starch waste water, potato starch waste water, sweet potato starch waste water, tapioca (flour) waste water, pueraria starch waste water.
7, method as claimed in claim 4 is characterized in that, described starch wastewater is corn starch wastewater, wheat starch waste water, potato starch waste water, sweet potato starch waste water, tapioca (flour) waste water, pueraria starch waste water.
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| CN100348514C true CN100348514C (en) | 2007-11-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363037B (en) * | 2008-08-27 | 2011-12-28 | 金翼 | Xanthan polysacchdride culture medium, preparation and application method thereof |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101108997B (en) * | 2006-07-19 | 2010-07-21 | 中国科学院大连化学物理研究所 | Process for preparing microbe oil |
| CN102876749A (en) * | 2012-09-21 | 2013-01-16 | 湖北梨花湖食品有限公司 | Deep processing method for corn processing residues |
| CN104445832B (en) * | 2014-12-18 | 2016-08-10 | 陕西科技大学 | Potato starch processing factory method of wastewater treatment |
| CN112655777A (en) * | 2021-01-11 | 2021-04-16 | 程凯 | Healthy compound olive oil and preparation method thereof |
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|---|---|---|---|---|
| CN1069070A (en) * | 1991-07-30 | 1993-02-17 | 中国科学院沈阳应用生态研究所 | Method for preparing gamma-linolenic acid and biological preparation mainly containing gamma-linolenic acid |
| CN1223079A (en) * | 1997-11-18 | 1999-07-21 | 河南莲花味之素有限公司 | Process for extracting protein from waster water of starch |
| JP2003310291A (en) * | 2002-04-26 | 2003-11-05 | Suntory Ltd | Method for producing lipid containing highly- unsaturated fatty acid |
-
2005
- 2005-03-29 CN CNB2005100114982A patent/CN100348514C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069070A (en) * | 1991-07-30 | 1993-02-17 | 中国科学院沈阳应用生态研究所 | Method for preparing gamma-linolenic acid and biological preparation mainly containing gamma-linolenic acid |
| CN1223079A (en) * | 1997-11-18 | 1999-07-21 | 河南莲花味之素有限公司 | Process for extracting protein from waster water of starch |
| JP2003310291A (en) * | 2002-04-26 | 2003-11-05 | Suntory Ltd | Method for producing lipid containing highly- unsaturated fatty acid |
Non-Patent Citations (2)
| Title |
|---|
| 多不饱和脂肪酸的研究进展 阮征等.中国油脂，第28卷第2期 2003 * |
| 微生物生产多价不饱和脂肪酸 罗明典.精细与专用化学品，第18卷 2003 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363037B (en) * | 2008-08-27 | 2011-12-28 | 金翼 | Xanthan polysacchdride culture medium, preparation and application method thereof |
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