CN107354098A - A kind of common harvesting method of microalgae cultivated using filamentous algae when similar - Google Patents
A kind of common harvesting method of microalgae cultivated using filamentous algae when similar Download PDFInfo
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- CN107354098A CN107354098A CN201710670488.2A CN201710670488A CN107354098A CN 107354098 A CN107354098 A CN 107354098A CN 201710670488 A CN201710670488 A CN 201710670488A CN 107354098 A CN107354098 A CN 107354098A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to a kind of common harvesting method of microalgae cultivated using filamentous algae when similar, belong to biotechnology or food technology field, hardly possible, with that can form algae group complex during unicellular micro algae growth, is easily collected unicellular alga in culture systems and realizes efficient harvest by the present invention using thread algae；The yield rate of microalgae is brought up to 97% by the method for the present invention；The method of the present invention need not deliberately increase the difference of the culture medium composition between inhomogeneity species, enormously simplify culture link and improve collection efficiency.
Description
Technical field
The present invention relates to a kind of common harvesting method of microalgae cultivated using filamentous algae when similar, belong to biotechnology or food
Technical field.
Background technology
The efficient harvest of microalgae biomass is the premise that downstream is processed trans-utilization.The autotrophy microalgae of common commercial
Concentration is cultivated between 0.3-0.5 g/L, moisture content is very big.Simultaneously microalgae volume very little, diameter probably 1 to 30 μm it
Between, how negatively charged frustule surface is, and individual can relatively evenly disperse to suspend in nutrient solution, form stable dispersion,
It is difficult to settle.These increase difficulty to microalgae harvesting, and harvest cost is very high.In general harvest into instinct and account for microalgae production assembly
This 20-30%.Traditional microalgae harvesting method mainly includes：Centrifugation, dissolved air flotation, flocculation separate with membrane filtration.Should be according to
According to algae Species differences, different concentration of algae convenient separation method is selected with condition of culture.
Centrifugal process efficiency is higher, cost is also higher, is most widely used a kind of collecting method in amplification culture microalgae.It is beautiful
State ASP studies title in the works, and microalgae is harvested by centrifugation cost estimation and accounts for the 40% of total cost of production, accounts for the 50% of capital invested.It is super
Filter technology is used for the concentration of microalgae, and cost of investment is big, operating cost is high, it is necessary to selects suitable operating pressure；Pressure is too small,
Then the operating time is grown, and production efficiency is not high, if pressure is too big, can influence the activity of cell again, it is also possible to cause milipore filter
Damage.In algae solution, the absorption deposition of gel solute molecule can cause filter membrane to pollute.Need often to carry out filter membrane cleaning.Flocculence
Refer to add flocculant in nutrient solution, the frustule of suspension is produced flocculating setting so as to harvest.Flocculation can be divided into chemical wadding
Solidifying, bioflocculation and electric flocculation.Although chemical flocculation efficiency high, excessive chemical reagent such as metal ion Al3+、Fe2+Deng meeting
Absorption is unfavorable to downstream processes in frustule；Bioflocculation can avoid toxic reagent absorption frustule from utilizing downstream
Impact, but cost is higher.
In summary, find a kind of microalgae harvesting method of green high-efficient be have it is very necessary.Low energy consumption microalgae harvesting
Technology is to realize one of major issue that algal biomass low cost is commercially produced.
The content of the invention
It is an object of the invention to overcome technological deficiency of the prior art, propose that one kind realizes that the growth of algae bio amount is tired
Product harvests progress one simultaneously with algal biomass and couples new technology.
To achieve the above objectives, the technological means that the present invention uses is as follows：
The present invention provides a kind of microalgae cultivated using filamentous algae when similar harvesting method altogether, and methods described is the culture in microalgae
Thread algae is added in system to be co-cultured to reach the method for improving microalgae harvesting rate.
Wherein described filamentous algae class is Huang Sizao, water silk floss, anabena etc., the difficult collection of microalgae it is unicellular or how thin
Born of the same parents algae colony；More further, the microalgae is Chlorophyta chlorella, Chlorophyta grid, micro- plan ball algae etc..
The material such as algae secretion algal polysaccharides, albumen forms algae group complex during the co-cultivation, to realize from training
Harvest is separated in the system of supporting.
Fe is such as added by the physicochemical environment factor of regulation culture system during the co-cultivation2+、Ca2+、Mg2+, grape
The formation of the condition such as the materials such as sugar or temperature, pH, light intensity, ultrasound induction algae group complex.
Accelerate the shape of algae group precipitation ball during the co-cultivation using concussion shaking table, bubble type or airlift reactor
Into.
In addition, all changes that one of ordinary skill in the art directly exports or associated from present disclosure
Shape, it is considered as protection scope of the present invention.
Compared with prior art, it is the device have the advantages that as follows：
The present invention realizes the microalgae cultivated with filamentous algae when similar and harvests technology altogether, and collection method is co-cultured with algae fungi etc.
Compare, the present invention need not deliberately increase the difference of the culture medium composition between inhomogeneity species, enormously simplify culture link and carry
High collection efficiency.With existing conventional centrifugal, flocculation collection method compared to also with cost of investment is small, operating cost is cheap, no
Need to select suitable operating pressure；Avoid toxic reagent absorption frustule to downstream using impacting, energy consumption is low, and microalgae is received
It is good to collect effect.
Embodiment
Below according to specific embodiment to the more preferable elaboration of technical scheme, so that those skilled in the art are more preferable
Understanding the present invention.
Embodiment 1：
Chlorella, Huang Sizao are co-cultured in BG11 nutrient solutions；Chlorella strainChlorellaSp. with yellow silk algae kindTribonemaSp. be purchased from Chinese Academy of Sciences's algae kind storehouse, take the algae kind of chlorella and yellow silk algae exponential phase by
10%（V/V）It is inoculated with, BG11 nutrient solutions is sterilized, chlorella, Huang Sizao, two kinds of mixed algaes is then respectively connected to BG11
Nutrient solution, it is appropriate to adjust culture medium composition Mg in 25 DEG C of illumination constant-temperature table cultures2+：0.25 mM, the d of continuous illumination culture 7
Afterwards, find to add the collected rate for co-culturing and can significantly improving chlorella；Compared with individually culture, optimal chlorella yield rate
80.2% is brought up to from 41.9%.
Embodiment 2：
Chlorophyta chlorellaChlorella vulgarisWith Cyanophyta anabenaAnabeana sp.It is purchased from the Chinese Academy of Sciences
Algae kind storehouse；Algae kind is cultivated respectively using sterile BG11 medium cultures base.1.0 are transferred to from the algae solution of activation
In L pillar Photoreactor, volume about 10% is inoculated with.Light source provides for the incandescent lamp tube positioned at pillar Photoreactor side（Light
By force: 200±50 μmol/(m2·s)）.Air agitation relies on the glass tube of intercalation reaction device bottom, is passed through containing 5% -6%
CO2, the vvm of throughput 1.0, adjusted by gas flowmeter.25 ± 1 °C of air-conditioning regulations of cultivation temperature, Fe2+：0.1–0.5mM.Even
After the continuous d of illumination cultivation 7, compared with individually culture, optimal chlorella yield rate brings up to 90.8% from 21.2%.
Embodiment 3：
Chlorophyta grid algaeScenedesmus obliquusWith Huang SizaoTribonemaSp. it is purchased from Chinese Academy of Sciences algae
Kind storehouse, the BG11 medium cultures base that 5%-20% glucose is added using sterilizing are cultivated algae kind respectively；From the algae of activation
Liquid is transferred in 1.0 L pillar Photoreactor, is inoculated with volume about 10%.Air agitation relies on the glass of intercalation reaction device bottom
Pipe, is passed through air, throughput is about 0.5 vvm, is adjusted by gas flowmeter.25 ± 1 °C of cultivation temperature, air-conditioning regulation.Even
After 10 d of continuous culture.Compared with individually culture, the optimal yield rate of grid algae brings up to 97% from 40.2%.
Claims (6)
1. the common harvesting method of a kind of microalgae cultivated using filamentous algae when similar, it is characterised in that methods described is in microalgae
Thread algae is added in cultivating system to be co-cultured to reach the method for improving microalgae harvesting rate.
2. according to the method for claim 1, it is characterised in that the thread algae is Huang Sizao, anabena, water silk floss etc.,
The unicellular or multicellular algae colony of the difficult collection of microalgae.
3. according to the method for claim 2, it is characterised in that the microalgae is Chlorophyta chlorella, grid algae, intends ball algae
Deng.
4. according to the method for claim 1, it is characterised in that methods described is thread to be added in the cultivating system of microalgae
Algae induces the materials such as algae secretion algal polysaccharides, albumen to form algae group complex during being co-cultured, to realize from training
Harvest is separated in the system of supporting.
5. according to the method for claim 1, it is characterised in that pass through the reason of regulation culture system during the co-cultivation
Change envirment factor and such as add Fe2+、Ca2+、Mg2+, the condition such as material or temperature, pH, light intensity, ultrasound such as glucose induction algae group it is multiple
Fit formation.
6. according to the method for claim 1, it is characterised in that concussion shaking table, bubble type are used during the co-cultivation
Or airlift reactor accelerates the formation of algae group precipitation ball.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710670488.2A CN107354098A (en) | 2017-08-08 | 2017-08-08 | A kind of common harvesting method of microalgae cultivated using filamentous algae when similar |
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| CN201710670488.2A CN107354098A (en) | 2017-08-08 | 2017-08-08 | A kind of common harvesting method of microalgae cultivated using filamentous algae when similar |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109430651A (en) * | 2018-11-26 | 2019-03-08 | 江苏科技大学 | A kind of chlorella health beverages and preparation method thereof |
| CN109536484A (en) * | 2018-11-23 | 2019-03-29 | 江苏大学 | A method of yellow silk algae biomass and metabolin are improved using magnetic field intervention |
| CN110282747A (en) * | 2019-07-05 | 2019-09-27 | 宁波大学 | A method of processing marine culture wastewater is co-cultured using Filamentous sheath silk algae and bait micro-algae chlorella |
| US20210146308A1 (en) * | 2018-04-04 | 2021-05-20 | Clariant International Ltd. | Process for the purification of complex biocompositions |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513846A (en) * | 2014-12-23 | 2015-04-15 | 清华大学 | Method for preparing microalgae biodiesel from oil-production green alga collected through self-floating of hydrogen-production blue alga |
| CN105638425A (en) * | 2016-01-18 | 2016-06-08 | 中国科学院水生生物研究所 | Space-adjustable aquatic plant co-culture device |
| CN106350455A (en) * | 2016-09-18 | 2017-01-25 | 南昌大学 | Hyphae pellet mediated fast microalga harvesting method |
-
2017
- 2017-08-08 CN CN201710670488.2A patent/CN107354098A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513846A (en) * | 2014-12-23 | 2015-04-15 | 清华大学 | Method for preparing microalgae biodiesel from oil-production green alga collected through self-floating of hydrogen-production blue alga |
| CN105638425A (en) * | 2016-01-18 | 2016-06-08 | 中国科学院水生生物研究所 | Space-adjustable aquatic plant co-culture device |
| CN106350455A (en) * | 2016-09-18 | 2017-01-25 | 南昌大学 | Hyphae pellet mediated fast microalga harvesting method |
Non-Patent Citations (6)
| Title |
|---|
| JIANGUO ZHANG 等: "A novel method to harvest microalgae via co-culture of filamentous fungi to form cell pellets", 《BIORESOURCE TECHNOLOGY》 * |
| OLGA TIRON 等: "Overcoming Microalgae Harvesting Barrier by Activated Algae Granules", 《SCIENTIFIC REPORTS》 * |
| WENGUANG ZHOU 等: "Filamentous fungi assisted bio-flocculation: A novel alternative technique for harvesting heterotrophic and autotrophic microalgal cells", 《SEPARATION AND PURIFICATION TECHNOLOGY》 * |
| Y. WANG 等: "Optimization of Chlorella vulgaris and bioflocculant producing bacteria co-culture: enhancing microalgae harvesting and lipid content", 《LETTERS IN APPLIED MICROBIOLOGY》 * |
| 樊华 等: "微藻生物采收技术的现状和展望", 《生物学杂志》 * |
| 马志欣 等: "微藻絮凝采收的研究进展", 《基因组学与应用生物学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210146308A1 (en) * | 2018-04-04 | 2021-05-20 | Clariant International Ltd. | Process for the purification of complex biocompositions |
| CN109536484A (en) * | 2018-11-23 | 2019-03-29 | 江苏大学 | A method of yellow silk algae biomass and metabolin are improved using magnetic field intervention |
| CN109536484B (en) * | 2018-11-23 | 2022-06-21 | 江苏大学 | Method for improving biomass and metabolite of chrysophyceae by magnetic field intervention |
| CN109430651A (en) * | 2018-11-26 | 2019-03-08 | 江苏科技大学 | A kind of chlorella health beverages and preparation method thereof |
| CN110282747A (en) * | 2019-07-05 | 2019-09-27 | 宁波大学 | A method of processing marine culture wastewater is co-cultured using Filamentous sheath silk algae and bait micro-algae chlorella |
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