CN104513846A - Method for preparing microalgae biodiesel from oil-production green alga collected through self-floating of hydrogen-production blue alga - Google Patents
Method for preparing microalgae biodiesel from oil-production green alga collected through self-floating of hydrogen-production blue alga Download PDFInfo
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- CN104513846A CN104513846A CN201410811948.5A CN201410811948A CN104513846A CN 104513846 A CN104513846 A CN 104513846A CN 201410811948 A CN201410811948 A CN 201410811948A CN 104513846 A CN104513846 A CN 104513846A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a method for preparing microalgae biodiesel from oil-production green alga collected through self-floating of hydrogen-production blue alga. The method comprises the following steps: first, cells of hydrogen-production microalga and oil-production microalga are cultured; then the hydrogen-production glue-green alga and the oil-production green alga are subjected to co-induction regulation in a certain proportion for hydrogen production and oil production, the cells of the hydrogen-production microalga and the oil-production microalga are collected through self-floating, and the purity analysis and rationing of hydrogen and extraction of microalgae oil are conducted; finally, the hydrogen produced through microalgae photosynthesis is directly added to the microalgae oil for preparation of high-quality biodiesel. The oil-production green alga are collected through the phenomenon that the filiform nitrogen-fixing blue alga can float automatically during hydrogen production; compared with a traditional microalgae collection method, the method does not need addition of flocculants or ventilation, does not need aiding of other high-energy-consumption equipment either, and solves the problem that the microalgae collection cost is excessively high. Meanwhile, the high-quality biofuel can be obtained through reaction of hydrogen and oil produced by the alga, and the method has vital practical significance and economic efficiency.
Description
Technical field
The invention belongs to bioenergy preparing technical field, be specifically related to a kind of method utilizing product hydrogen blue-green algae to prepare microalgae biodiesel from floating produce oil green alga of gathering.
Background technology
Society, fossil energy exhaustion, scarcity of fresh water resources and Greenhouse effect seriously govern society and expanding economy, in the urgent need to finding efficient, clean, free of contamination new green power.Micro-algae, as a kind of fast growth, crop that oil offtake is high, less to the Resource Dependence such as fresh water and arable land, attracts the sight of global energy scholar and environmentalist gradually.
In extensive both culturing microalgae, gathering of frustule is a key link.Due to microalgae cell individuality small (3-30 μm), and concentration in nutrient solution is very low, determines its difficulty of gathering larger.According to the literature, its cost accounts for the 20-30% of total cost of production.Gathering of biomass is exactly remove the free water in algae liquid, reduces the process of frustule water content, for the links such as follow-up grease extraction are prepared.Therefore, effectively the rate of recovery of raising microalgae cell, the water content of reduction frustule not only can improve the efficiency that biomass are gathered, and can also arrive at energy consumption and the cost of subsequent technique, significant.
Traditional microalgae cell collecting method mainly contains centrifuging, filtration method, flocculation sedimentation and By Bubble-floating Method etc.In addition, also there is the biomass percentage recovery that some are emerging, if supersonic method, biotransformation method etc. are also in constantly researching and developing.
Centrifuging processing speed is fast, also less to the damage of frustule, and the biomass rate of recovery high (> 90%), anhydrate effective, applied widely, but the energy consumption of whizzer is almost the highest in all separating devices, and treatment capacity also can be subject to machine size and number quantitative limitation, and this separation method causes the results of micro-algae almost to occupy the 1/3-1/2 of microdisk electrode cost.
Filtration method is conventional, ripe chemical industry method.But ordinary filter paper aperture is excessive, microalgae cell only has 3-10 μm, and it is more difficult to filter.If change filter membrane into, even if pore size is suitable, but a lot of cell has viscosity, easily pollutes, blocking filter membrane, and cause disengaging time long, even filtrate cannot be passed through, if increase pressure, cost raises on the one hand, and physical strength is on the other hand inadequate.In addition, because the density of micro-algae suspension is too low, need lot of energy just algae liquid pump can be entered filtration unit, realize the object of filtering separation, high cost.
Flocculation sedimentation is by regulating pH or add inorganic or organic compound in substratum, making micro-algae generation flocculating settling, realizes algae water and is separated.But the flocculation agent added probably produces destruction to micro-algae, and in follow-up micro-algae course of processing, owing to must extract the flocculation agent in institute's harvesting microalgae, adds numerous and diverse degree of technique.In addition, can produce micro algae growth when some residual flocculant concentration is too high and poison, cost is higher again for the polysaccharide flocculant of some safety non-toxic.
By Bubble-floating Method is the more potential micro-algae enriching method of one, but the method needs rational air-flotation separation equipment and separating technology, and separating effect is subject to the many factors such as device structure and processing condition.
The research such as Ming Chen shows, thread Azotica can complete from floating in product hydrogen process, from floating rate up to 91.71%, greatly reduces the results cost of micro-algae.
Summary of the invention
The object of the present invention is to provide a kind of method utilizing product hydrogen blue-green algae to prepare microalgae biodiesel from floating produce oil green alga of gathering, the hydrogen produced by blue-green algae photosynthesis is completed from produce oil green alga that is floating and Dual culture of gathering simultaneously, and the hydrogen of generation and algae oil carry out direct hydrogenation and prepares high quality microalgae biofuel.
The technical solution adopted in the present invention is:
Utilize the method for producing hydrogen blue-green algae and preparing microalgae biodiesel from floating produce oil green alga of gathering, comprise step as follows:
(1) product hydrogen blue-green algae and produce oil green alga are cultivated;
(2) product hydrogen blue-green algae, produce oil green alga are carried out to common induction regulating controlling and produce hydrogen, produce oil, by from floating two kinds of frustules of gathering, and carry out the purity check, quantitatively and the extraction of microalgae grease of hydrogen;
(3) H producing the photosynthetic generation of hydrogen blue-green algae is utilized
2directly to micro-algae oil Hydrogenation for biofuel.
Wherein, carry out the cultivation of producing hydrogen blue-green algae, produce oil green alga before coinduction, culturing process basic medium used is BGII or TAP substratum.Constant temperature in culturing process also adopts 24h continuous light, and liquid ventilation is cultivated.Algae liquid is placed in 30 DEG C of temperature and 30 μm of ol photon m
– 2s
– 1under (Model MQ-100) intensity of illumination, and pass to CO
2: Air=2:98 (v:v) mixed gas.
Produce hydrogen, produce oil efficiency reach from floating to improve, optionally, producing the algae kind that hydrogen process adopts is the thread Azotica that hydrogen generation efficiency is higher, and the algae kind that produce oil process adopts is the unicell green alga that in cell, fat content is higher.
The method of described common induction is: product hydrogen blue-green algae, produce oil green alga are cultured to late log phase OD respectively
750value mixes for during 0.8-0.95, and coinduction condition is more than one in auxotrophy induction, anaerobic induction, dark induction.
Product hydrogen blue-green algae, produce oil green alga mixed volume ratio are 10:1-1:10.
After cultivation terminates, High-pressure gas chromatography can be utilized to carry out purity check with quantitative to hydrogen, gather from floating frond, extract the grease in microalgae cell, direct hydrogenation production biofuel.
The high-quality biological diesel oil that main component is hydro carbons is obtained after reaction terminates.
The advantage of the inventive method compared with additive method is:
(1) the collecting method technique that adopts of the inventive method is simple and quick, does not need to add special biomass recovering device and can complete and gather from floating.
(2) collecting method that adopts of the inventive method is compared with other traditional technologys, does not need the equipment relying on high energy consumption, only relies on frond self to produce hydrogen and completes from floating results, greatly reduce the energy consumption of recovery process.
(3) the inventive method utilizes thread Azotica from the produce oil green alga of floating Dual culture of gathering, and pass through from hydrogen product and grease production high quality microalgae biofuel, profound conversion is carried out to the sun power be stored in microalgae cell, fundamentally improve transformation efficiency, the collecting method adopted does not need to add any physics or chemical floc, also do not need can complete the sepn process of algae water by regulating pH or adding metal-salt, without subsequent treatment process, and environmentally safe.
(4) microalgae biodiesel is prepared by the inventive method, the starting material hydrogen utilizing micro-algae self-produced and algae oil react, there is higher using value, have wide potential market, significant to the research of microalgae biodiesel, there is higher potential economic benefit and social benefit.
Embodiment
Provide specific embodiment below, by embodiment, the present invention is specifically described.What be necessary to herein means out is; embodiment is only for being further detailed the present invention; can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential improvement and adjustment according to the content of the invention described above.
Embodiment 1
BGII culture medium culturing is utilized to produce hydrogen blue-green algae A7119, produce oil green alga Chlorella.Algae liquid is placed in 30 DEG C of temperature and 30 μm of ol photon m
– 2s
– 1under (Model MQ-100) intensity of illumination, and pass to CO
2: Air=2:98 (v:v) mixed gas.Product hydrogen blue-green algae, produce oil green alga are cultured to late log phase OD respectively
750when value is 0.8, according to the ratio of volume ratio 1:5, hydrogen, produce oil are produced to A7119 and Chlorella coinduction.The method of coinduction is auxotrophy induction; After cultivation terminates, utilize High-pressure gas chromatography that hydrogen is carried out to purity check, quantitatively, gathers, gather from two kinds of floating frustules, utilize the grease in chloroform/methanol method extraction microalgae cell.Carry out hydrogenation reaction, after reaction terminates, obtain the high-quality biological diesel oil that main component is hydro carbons.
Embodiment 2
BGII culture medium culturing is utilized to produce hydrogen blue-green algae A7119, produce oil green alga cc124.Algae liquid is placed in 30 DEG C of temperature and 30 μm of ol photon m
– 2s
– 1under (Model MQ-100) intensity of illumination, and pass to CO
2: Air=2:98 (v:v) mixed gas.Product hydrogen blue-green algae, produce oil green alga are cultured to late log phase OD respectively
750when value is 0.95, according to the ratio of volume ratio 1:2, hydrogen, produce oil are produced to A7119 and cc124 coinduction.The method of coinduction is anaerobic induction, after cultivation terminates, utilizes High-pressure gas chromatography that hydrogen is carried out to purity check, quantitatively, gathers, gathers from two kinds of floating frustules, utilize the grease in chloroform/methanol method extraction microalgae cell.Carry out hydrogenation reaction, after reaction terminates, obtain the high-quality biological diesel oil that main component is hydro carbons.
Embodiment 3
TAP culture medium culturing is utilized to produce hydrogen blue-green algae A7119, produce oil green alga cc503.Algae liquid is placed in 30 DEG C of temperature and 30 μm of ol photon m
– 2s
– 1under (Model MQ-100) intensity of illumination, and pass to CO
2: Air=2:98 (v:v) mixed gas.Product hydrogen blue-green algae, produce oil green alga are cultured to late log phase OD respectively
750when value is 0.9, according to the ratio of volume ratio 10:1, hydrogen, produce oil are produced to A7119 and cc503 coinduction.The method of coinduction is dark induction, after cultivation terminates, utilizes High-pressure gas chromatography that hydrogen is carried out to purity check, quantitatively, gathers, gathers from two kinds of floating frustules, utilize the grease in chloroform/methanol method extraction microalgae cell.Carry out hydrogenation reaction, after reaction terminates, obtain the high-quality biological diesel oil that main component is hydro carbons.
Claims (5)
1. utilize the method for producing hydrogen blue-green algae and preparing microalgae biodiesel from floating produce oil green alga of gathering, it is characterized in that, comprise step as follows:
(1) respectively product hydrogen blue-green algae and produce oil green alga are cultivated;
(2) product hydrogen blue-green algae, produce oil green alga are carried out to common induction regulating controlling and produce hydrogen, produce oil, by from floating two kinds of frustules of gathering, and carry out the purity check, quantitatively and the extraction of microalgae grease of hydrogen;
(3) H producing the photosynthetic generation of hydrogen blue-green algae is utilized
2directly to micro-algae oil Hydrogenation for biofuel.
2. method according to claim 1, is characterized in that, culturing process basic medium used is BGII or TAP substratum.
3. method according to claim 1, is characterized in that, described product hydrogen blue-green algae is thread Azotica, and described produce oil green alga is unicellular produce oil green alga.
4. method according to claim 1, is characterized in that, the method for described common induction is: product hydrogen blue-green algae, produce oil green alga are cultured to late log phase OD respectively
750value mixes for during 0.8-0.95, and coinduction condition is more than one in auxotrophy induction, anaerobic induction, dark induction.
5. method according to claim 4, is characterized in that, product hydrogen blue-green algae, produce oil green alga mixed volume ratio are 10:1-1:10.
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| CN201410811948.5A CN104513846A (en) | 2014-12-23 | 2014-12-23 | Method for preparing microalgae biodiesel from oil-production green alga collected through self-floating of hydrogen-production blue alga |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107354098A (en) * | 2017-08-08 | 2017-11-17 | 江苏大学 | A kind of common harvesting method of microalgae cultivated using filamentous algae when similar |
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| CN101280328A (en) * | 2008-05-27 | 2008-10-08 | 清华大学 | Method for producing biodiesel by autotrophic culture and heterotrophic culture of chlorella |
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| CN103571877A (en) * | 2013-10-14 | 2014-02-12 | 清华大学 | Novel and high-efficiency method for producing hydrogen by using blue-green alga and solar energy and application thereof |
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2014
- 2014-12-23 CN CN201410811948.5A patent/CN104513846A/en active Pending
Patent Citations (3)
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| CN101280328A (en) * | 2008-05-27 | 2008-10-08 | 清华大学 | Method for producing biodiesel by autotrophic culture and heterotrophic culture of chlorella |
| CN101368193A (en) * | 2008-10-14 | 2009-02-18 | 蔡志武 | Process for preparing fine algae cultivation coupling biological diesel oil refining |
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| CN107354098A (en) * | 2017-08-08 | 2017-11-17 | 江苏大学 | A kind of common harvesting method of microalgae cultivated using filamentous algae when similar |
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Application publication date: 20150415 |
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