CN106344628A - Application of osmunda japonica extract in preparation of antihypertensive drugs - Google Patents
Application of osmunda japonica extract in preparation of antihypertensive drugs Download PDFInfo
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- CN106344628A CN106344628A CN201610894528.7A CN201610894528A CN106344628A CN 106344628 A CN106344628 A CN 106344628A CN 201610894528 A CN201610894528 A CN 201610894528A CN 106344628 A CN106344628 A CN 106344628A
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- common vetch
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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Abstract
The invention relates to an application of an osmunda japonica extract in preparation of antihypertensive drugs, belonging to the technical field of food deep processing and application. According to the application of an osmunda japonica extract in preparation of antihypertensive drugs, the osmunda japonica extract comprises 40-50% of ecdysterone, 12% of amino acids and 8% of tannic acid all by mass, wherein the amino acids include threonine, valine, methionine, leucine, lysine, isoleucine and phenylalanine in a mass ratio of 2:3:3:2:4:5:6. The invention provides an osmunda japonica extract which has the advantages of clear composition, good quality, controllable quality and wide application as well as multiple physiological and pharmacological activities; and the osmunda japonica extract is applied to antihypertensive drugs and realizes a good antihypertensive effect.
Description
Technical field
The present invention relates to wild vegetable processing and utilization, the specially common vetch dish extract application in preparation blood pressure lowering medicine.
Background technology
Living standard with people and the raising of health level, people increasingly note nutritive value of food and dietetic therapy
It is worth, pollution-free food is favored by people very much, and wild vegetable is exactly one type.Wild vegetable not only has higher edibility, also
There is certain value medical health care.In China, edible wild herbs are with a history of thousands of years.Hill vegetable food is nuisanceless, pollution-free,
Edible safety.Edible wild vegetable, as the supply source of vitamin, constant or trace element, has additional nutrients, good for health.
Common vetch dish is the tender leaf handle of pteridophyta osmund, is one kind of wild vegetable, with not deployed sporinite tender leaf for food, battalion
Support abundant, delicious flavour, there is higher edible health and be worth；The effect of tool flat stomach of promoting blood circulation, bright knowledge, be wild draft
Plant.Common vetch dish is nutritious, and bitter in the mouth is cool in nature, have calm the nerves, blood pressure lowering, anticancer, heat clearing away, fat-reducing the effects such as, common vetch dish matter is crisp and few fibre
Dimension, delicious tasty and refreshing, nutritious.Its protein, inorganic salt, ascorbic acid content are above more than ten times of common green vegetable.Additionally, common vetch
Dish also has the trace element such as ecdysone, aminoacid, tannic acid and potassium, calcium, phosphorus, ferrum, zinc.Being fabricated to dehydration salted Brassicae siccus common vetch dish more
Or canned food, to being confined to change in edible way the processing of common vetch dish more, but what needleless developed and utilized to its pharmacological action
Research, the many constituents to common vetch dish and function understand less, especially hypotensive effect, are to expand common vetch dish deep processing and utilization
Important directions.
Content of the invention
The technical problem to be solved in the present invention is to extract beneficiating ingredient from common vetch dish and applied in antihypertensive drugs.
In order to solve above-mentioned technical problem, technical scheme is as follows: common vetch dish extract is in preparation blood pressure lowering medicine
Application, in described common vetch dish extract: the mass percent of ecdysone is 40-50%, and the mass percent of aminoacid is
12%, the mass percent of tannic acid is 8%；
Described aminoacid includes threonine, L-Valine, Methionine, leucine, lysine, isoleucine and phenylpropyl alcohol ammonia
Acid, the mass ratio between described threonine, L-Valine, Methionine, leucine, lysine, isoleucine and Phenylalanine is
2:3:3:2:4:5:6；
The preparation method of described common vetch dish extract comprises the steps:
(1) pulverize dry for Dried Osmund as raw material, be placed in separating still, carry out supercritical fluid extraction: extractant is
co2, extracting pressure is 55-60mpa, and extraction temperature is 25 DEG C, and separation temperature is 30 DEG C, and separating pressure is 9mpa, and circulation is dynamic
Extraction 6h；Obtain extract；
(2) obtain common vetch dish extract by freeze-dried for extract.
Further, the mass percent of described ecdysone is 45%.
Further, described extracting pressure is 55mpa.
Further, common vetch dish extract can be prepared into compositionss, be then added in medicine, described compositionss are by 0.01-
The common vetch dish extract of 99.9wt% and the acceptable carriers composition as surplus.Described carrier includes but is not limited to: buffer,
Glucose, glycerol, ethanol or combinations thereof.
The mode of the medicine preferred oral of the present invention is administered, and usage amount is pressed common vetch dish extract and calculated, usually daily about 20-
50g.Additionally, the preparation of the present invention can also be used together with other therapeutic agents, other therapeutic agents are various existing depressor.
Beneficial effects of the present invention are as follows:
(1) provide one kind to form distinct, best in quality, quality controllable, of many uses and there is multiple physiology and pharmacology
The common vetch dish extract of activity；And applied in antihypertensive drugs, there is good antihypertensive effect；
(2) pass through supercritical co2Fluid extraction technology achieves the deep processing and utilization of common vetch dish, in extraction process, extraction
Pressure and temperature and detached temperature and pressure serve decisive action to the ratio of beneficiating ingredient in common vetch dish extract, this
The extraction conditionss of bright employing are: extractant is co2, extracting pressure is 55-60mpa, and extraction temperature is 25 DEG C, and separation temperature is 30
DEG C, separating pressure is 9mpa so that each one-tenth in common vetch dish extract is grouped into distinct, reasonable mixture ratio；And simple to operate, obtain
Common vetch dish extract quality is good, stay in grade；
(3) in the common vetch dish extract that the present invention extracts ecdysone, aminoacid and tannic acid reasonable mixture ratio, and amino
Acid includes 7 kinds of essential amino acids, and each beneficiating ingredient cooperates performance hypotensive effect.
Specific embodiment
With reference to specific embodiment, technical scheme is further described, but the present invention is not with any shape
Formula is limited to embodiment content.Test method described in embodiment if no special instructions, is conventional method；As no special theory
Bright, described reagent and biomaterial, all commercially obtain.
Embodiment 1
(1) Operation in Dandong Area taken from by the present embodiment common vetch dish, is ground into 30 mesh powder after natural drying, and the water content of powder is
3%.
(2) common vetch vegetable powder end is placed in separating still, carries out supercritical fluid extraction: extractant is co2, extracting pressure is
55mpa, extraction temperature is 25 DEG C, and separation temperature is 30 DEG C, and separating pressure is 9mpa, circulates dynamic extraction 6h；Obtain extract.
(3) obtain common vetch dish extract by freeze-dried for extract.
In the common vetch dish extract of the present embodiment: the mass percent of ecdysone is 40%, the percent mass of aminoacid
Number is 12%, and the mass percent of tannic acid is 8%；
Described aminoacid includes threonine, L-Valine, Methionine, leucine, lysine, isoleucine and phenylpropyl alcohol ammonia
Acid, the mass ratio between described threonine, L-Valine, Methionine, leucine, lysine, isoleucine and Phenylalanine is
2:3:3:2:4:5:6.
Common vetch dish extract is prepared into compositionss, common vetch dish extract (0.01wt%) is dissolved in the mixed of phosphate buffer and ethanol
Close in liquid.
Embodiment 2
(1) Operation in Dandong Area taken from by the present embodiment common vetch dish, is ground into 30 mesh powder after natural drying, and the water content of powder is
4%.
(2) common vetch vegetable powder end is placed in separating still, carries out supercritical fluid extraction: extractant is co2, extracting pressure is
60mpa, extraction temperature is 25 DEG C, and separation temperature is 30 DEG C, and separating pressure is 9mpa, circulates dynamic extraction 6h；Obtain extract.
(3) obtain common vetch dish extract by freeze-dried for extract.
In the common vetch dish extract of the present embodiment: the mass percent of ecdysone is 45%, the percent mass of aminoacid
Number is 12%, and the mass percent of tannic acid is 8%；
Described aminoacid includes threonine, L-Valine, Methionine, leucine, lysine, isoleucine and phenylpropyl alcohol ammonia
Acid, the mass ratio between described threonine, L-Valine, Methionine, leucine, lysine, isoleucine and Phenylalanine is
2:3:3:2:4:5:6.
Common vetch dish extract is prepared into compositionss, common vetch dish extract (99.9wt%) is dissolved in glucose solution.
Embodiment 3
(1) Operation in Dandong Area taken from by the present embodiment common vetch dish, is ground into 30 mesh powder after natural drying, and the water content of powder is
3.5%.
(2) common vetch vegetable powder end is placed in separating still, carries out supercritical fluid extraction: extractant is co2, extracting pressure is
55mpa, extraction temperature is 25 DEG C, and separation temperature is 30 DEG C, and separating pressure is 9mpa, circulates dynamic extraction 6h；Obtain extract；
(3) obtain common vetch dish extract by freeze-dried for extract.
In the common vetch dish extract of the present embodiment: the mass percent of ecdysone is 45%, the percent mass of aminoacid
Number is 12%, and the mass percent of tannic acid is 8%；
Described aminoacid includes threonine, L-Valine, Methionine, leucine, lysine, isoleucine and phenylpropyl alcohol ammonia
Acid, the mass ratio between described threonine, L-Valine, Methionine, leucine, lysine, isoleucine and Phenylalanine is
2:3:3:2:4:5:6.
Common vetch dish extract is prepared into compositionss, common vetch dish extract (50wt%) is dissolved in phosphate buffer.
Embodiment 4
The raw material of the present embodiment, with embodiment 3, differs only in the condition of extraction.
The extraction conditionss of the present embodiment are: extractant is co2, extracting pressure is 30mpa, and extraction temperature is 50 DEG C, separates
Temperature is 50 DEG C, and separating pressure is 4mpa, circulates dynamic extraction 6h；Obtain extract.
In common vetch dish extract by the preparation of this extraction conditions: the mass percent of ecdysone is 30%, aminoacid
Mass percent is 51%, and the mass percent of tannic acid is 2%；
Aminoacid includes threonine, L-Valine, Methionine, leucine, lysine, isoleucine, described threonine, figured silk fabrics
Mass ratio between propylhomoserin, Methionine, leucine, lysine, isoleucine is 3:4:1:2:5:2.
From the present embodiment result, extraction conditionss have significant impact to the composition of common vetch dish extract and proportioning, control extraction
Pressure power is 55-60mpa, and extraction temperature is 25 DEG C, and separation temperature is 30 DEG C, and separating pressure is 9mpa, circulates dynamic extraction 6h；
The extract component content obtaining is clear, reasonable mixture ratio.
Common vetch dish extract is prepared into compositionss, common vetch dish extract (50wt%) is dissolved in phosphate buffer.
Embodiment 5
The compositionss that embodiment 3 is prepared with embodiment 4 carry out animal experiment respectively
Laboratory animal: shr (spontaneous hypertensive rat) male Body Weight 275g
Test apparatuses: animal non-invasive blood pressure instrument
Shr rat is divided into three groups, makes to respectively the compositionss of each group shr rat oral gavage embodiment 3 preparation, embodiment 4
Standby compositionss and normal saline, gavage volume is 1ml/100g body weight.Respectively at administration pre-test basic blood pressure value 3 times, give
After medicine, 2 hours measurement rat blood pressure values are averaged for 3 times.Observe rat blood pressure situation of change.
Mean pressure result such as table 1 below:
From the above results, according to the common vetch dish extract of technical scheme preparation, there is good reducing blood pressure
Effect.
Claims (4)
1. application in preparation blood pressure lowering medicine for the common vetch dish extract is it is characterised in that in described common vetch dish extract: ecdysone
Mass percent be 40-50%, the mass percent of aminoacid is 12%, and the mass percent of tannic acid is 8%；
Described aminoacid includes threonine, L-Valine, Methionine, leucine, lysine, isoleucine and Phenylalanine, institute
Mass ratio between threonine, L-Valine, Methionine, leucine, lysine, isoleucine and the Phenylalanine stated is 2:3:3:
2:4:5:6；
The preparation method of described common vetch dish extract comprises the steps:
(1) pulverize dry for Dried Osmund as raw material, be placed in separating still, carry out supercritical fluid extraction: extractant is co2, extraction
Pressure is 55-60mpa, and extraction temperature is 25 DEG C, and separation temperature is 30 DEG C, and separating pressure is 9mpa, circulates dynamic extraction 6h；?
Extract；
(2) obtain common vetch dish extract by freeze-dried for extract.
2. application in preparation blood pressure lowering medicine for the common vetch dish extract as claimed in claim 1 is it is characterised in that described rush takes off
The mass percent of corticosterone is 45%.
3. application in preparation blood pressure lowering medicine for the common vetch dish extract as claimed in claim 1 is it is characterised in that described extraction
Pressure is 55mpa.
4. application in preparation blood pressure lowering medicine for the described common vetch dish extract as arbitrary in claim 1-3 is it is characterised in that by common vetch
Dish extract is prepared into compositionss, and described compositionss are by the common vetch dish extract of 0.01-99.9wt% and being subjected to as surplus
Carrier forms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610894528.7A CN106344628B (en) | 2016-10-13 | 2016-10-13 | Application of the common vetch dish extract in preparation decompression drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610894528.7A CN106344628B (en) | 2016-10-13 | 2016-10-13 | Application of the common vetch dish extract in preparation decompression drug |
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| Publication Number | Publication Date |
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| CN106344628A true CN106344628A (en) | 2017-01-25 |
| CN106344628B CN106344628B (en) | 2019-09-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610894528.7A Active CN106344628B (en) | 2016-10-13 | 2016-10-13 | Application of the common vetch dish extract in preparation decompression drug |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105614627A (en) * | 2014-10-27 | 2016-06-01 | 哈尔滨天一生态农副产品有限公司 | Osmunda japonica blood pressure lowering medicinal instant granules and preparation method thereof |
| CN105942034A (en) * | 2016-05-30 | 2016-09-21 | 青岛海之源智能技术有限公司 | Prochilodus scrofa advanced fry mixed feed and preparation method thereof |
-
2016
- 2016-10-13 CN CN201610894528.7A patent/CN106344628B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105614627A (en) * | 2014-10-27 | 2016-06-01 | 哈尔滨天一生态农副产品有限公司 | Osmunda japonica blood pressure lowering medicinal instant granules and preparation method thereof |
| CN105942034A (en) * | 2016-05-30 | 2016-09-21 | 青岛海之源智能技术有限公司 | Prochilodus scrofa advanced fry mixed feed and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 班丽敏: "薇菜中氨基酸及无机元素含量的分析", 《中国林副特产》 * |
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