CN106318876A - Ceriporia lacerata strain and culture method and application thereof - Google Patents
Ceriporia lacerata strain and culture method and application thereof Download PDFInfo
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- CN106318876A CN106318876A CN201510395180.2A CN201510395180A CN106318876A CN 106318876 A CN106318876 A CN 106318876A CN 201510395180 A CN201510395180 A CN 201510395180A CN 106318876 A CN106318876 A CN 106318876A
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Abstract
The invention relates to the field of industrial microorganisms and discloses a ceriporia lacerata strain. The ceriporia lacerata strain has a collection number of CGMCC No.10485. The invention also discloses a method for culturing the ceriporia lacerata strain. The method comprises a step of inoculating the ceriporia lacerata strain into a culture medium for culturing. In addition, the invention also discloses application of the ceriporia lacerata strain in preparation of degradable materials. The mycelium of the ceriporia lacerata strain disclosed by the invention is capable of winding and fixing scraps of plant medium materials. By virtue of the ceriporia lacerata strain disclosed by the invention, plant materials can be prepared into the degradable materials under the condition that sterilization or bacteriostatic treatment is not performed, an aseptic environment is not needed in the preparation process, and the capacity of resisting infectious microbes is high; and moreover, because the ceriporia lacerata strain disclosed by the invention has high capacity of resisting infectious microbes, the various culture processes (liquid culture or solid culture) can be performed under non-sterile conditions; and therefore, the preparation cost of the degradable materials can be greatly reduced.
Description
Technical field
The invention belongs to industrial microorganism field, relate to strain tear wax pore fungi (Ceriporia lacerata)
And cultural method and application.
Background technology
Foam plastics is common insulation, heat insulation and padded coaming, building, pack, fill, energy
Absorptions etc. are multi-field to be widely used.But, foam plastics faces two hang-ups: one be raw material from
Oil, as often produced 1m3Polystyrene foam plastics needs 65L oil, and oil is limited,
The dependence on external supply degree of China's oil has exceeded 60%, seeks the sub gesture of petroleum based foams plastics must
OK；Two is to be difficult to be decomposed in natural environment, serious harm ecological environment and living environment.Rivers lake
The plastic refuse being mingled with in the plastic refuse drifted about in pool and herbage is own to be caused Fish and domestic animal
Threaten.Such as, there are the animals such as substantial amounts of seabird dead because of edible discarded plastics every year.According to California
The investigation of seashore committee (California Coastal Commission), foam plastics has been main
Ocean drift, and for eating the marine organisms of this kind of plastic cement by mistake, its digestive system can be damaged,
But there is presently no any of organism can it be degraded.Plastic garbage not only affects environment
Attractive in appearance, and polluted source and soil, jeopardize poultry and wild animal, bring to ball ecological environment
Heavy burden.China processes a main approaches and methods of municipal refuse and fills exactly.On the one hand by
, volume little in foam plastic density is big, and selected place quickly can be filled up by it, processes with causing landfill yard
The ability of rubbish is greatly reduced, and environment causes bigger pollution；On the other hand, place is all filled
After, ground the most just becomes the softest, and the harmful substance such as antibacterial in rubbish, virus is easy to
It is impregnated into underground, polluted underground water, jeopardizes environment；When plastics burn, dioxin can be produced, burn bubble
Environment will be caused serious secondary pollution by foam plastic garbage.Due to the external resistance to foam plastics, produce
The foam plastics padded coaming that product packaging uses has had a strong impact on the outlet of some products of China.
For solving the problems referred to above, the research of bio-based degradation plastic is in the ascendant, but bio-based so far
Plastics, such as polylactic acid, PBS, PPC, biological PP, bio-based PET, biological poly amide and biology
Base polyethylene etc., are all with sugar as raw material, strive grain with people and animals；Even if with lignocellulose
(lignocellulose) it is fermentation raw material, also first has to lignocellulose is hydrolyzed to sugar, then pass through
Liquid submerged fermentation or be biologically converted into the monomer of synthetic plastic (PHA is to utilize sugar the most biosynthetic
Macromolecular material, is accumulated in intracellular after synthesis, therefore has to pass through technology for broken wall, could obtain thick PHA),
The most chemically it is polymerized to bio-based plastics.The consumption of such technique, raw material and the energy is big, technique
Route is long, complex manufacturing, and equipment investment is big, and product cost is high, synthesizes high molecular degradable plastics
Cost is higher than existing general-purpose plastics, production process heavy contamination.Therefore, this series products of present stage is the most difficult
With universal.
More ripe starch plastic obtains flourish from the eighties in last century, and U.S.'s annual rate of growth is the highest
Reach 75%, the most mainly filled-type starch-based degradable plastics.Starch-based degradable plastics be to
The class biodegradable plastic that the present develops at most, research unit both domestic and external is a lot, the only research of China
Unit just has more than 40.The result of study delivered successively from the nineties in 20th century shows, they are only
Degrade starch therein, other component only broken into pieces, it is still among soil and waters, can not
Fundamentally solve " white pollution " of plastics.
Terrestrial plant, in long-term evolutionary process, defines and by cellulose, hemicellulose and lignin is
The compact structure of main component and the cell wall of complexity, lignin is tight together with hemi-cellulose components knot
Be wrapped in fibrination sugar moieties, the mixture of this three dimensional structure is referred to as lignocellulose.Wooden
Cellulose is the maximum amount of renewable biomass, estimates that whole world annual production reaches 1 × 1010MT(Sánchez,
ó.J.,Cardona,C.A.,2008.Trends in biotechnological production of fuel ethanol
From different feedstocks.Bioresour.Technol.99,5270-5295.), it is human future
Preferable resource.At present, the reproducible wood fibre such as China's waste straw having up to 6-7 hundred million tons every year
Element resource is economically and effectively developed, and even becomes the burden of rural area and peasant, burns straw
Stalk remains incessant after repeated prohibition, and produces substantial amounts of PM 2.5, becomes the polluter that China is new.How by such flood tide
Straw is really converted into can be with the resource of the creation of value, and the strategy having become as Chinese society economic development is asked
Topic.
If can be with stalk resource as raw material, with novel fermentation technology low cost, contamination-freely manufacturing market
Capacity bio-based materials big, good in economic efficiency, eco-friendly, will adjust Industry Structure, very
To the economic and great significance of society.
Although, currently also there are some about the report utilizing microorganism to prepare bio-based materials, but, its
The liquid culture process related to and/or solid culture process are required to aseptically carry out, to cultivating bar
Part requires harshness, and the preparation cost of bio-based materials is high.
Summary of the invention
It is an object of the invention to provide one to may be used for preparing mycelium material and to condition of culture requirement
Low tear wax pore fungi and cultural method thereof and application.
To achieve these goals, the present inventor has carried out great many of experiments, and screening a strain can
The tear wax pore fungi of degradable bulk material prepared by the vegetalitas that is effectively fastened material.Therefore, first party
Face, the invention provides a kind of tear wax pore fungi (Ceriporia lacerata), the guarantor of this tear wax pore fungi
Hide numbered CGMCC No.10485.
Second aspect, a kind of method that the invention provides tear wax pore fungi cultivated described in first aspect,
The method includes being seeded in culture medium cultivate by the tear wax pore fungi described in first aspect.
The third aspect, the invention provides the tear wax pore fungi described in first aspect in preparation degradation material
In application.
The present invention tears the mycelium of wax pore fungi and can be fastened the chip of vegetalitas culture medium material, borrows
Helping the tear wax pore fungi of the present invention, vegetalitas material can be prepared in the case of the most sterilized or antibacterial process
Degradation material, and preparation process do not requires gnotobasis, the ability of opposing miscellaneous bacteria is strong.Due to the present invention
The ability of opposing miscellaneous bacteria of tear wax pore fungi strong, its various cultivations (liquid culture or solid culture) mistake
Cheng Jun can be carried out under conditions of non-sterile, so degradation material can be reduced to a great extent
Preparation cost.
Other features and advantages of the present invention will be described in detail in detailed description of the invention part subsequently.
Biological deposits
The tear wax pore fungi (Ceriporia lacerata) of the present invention, was preserved on April 28th, 2015
In (address: north, Chaoyang District, Beijing City, China Committee for Culture Collection of Microorganisms's common micro-organisms center
Occasion West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101) (preservation
Unit be abbreviated as CGMCC), deposit number is CGMCC No.10485.
Accompanying drawing explanation
Accompanying drawing is used to provide a further understanding of the present invention, and constitutes the part of description, with
Detailed description below is used for explaining the present invention together, but is not intended that limitation of the present invention.?
In accompanying drawing:
Fig. 1 is a kind of embodiment utilizing the tear wax pore fungi of the present invention to prepare degradation material method
Flow chart；
Fig. 2 is the tear wax pore fungi aspect graph after liquid culture of the present invention, and wherein, Fig. 2 A represents
Form after cultivating with the fluid medium of sterilizing, Fig. 2 B represents with the fluid medium training of sterilized
Form after Yanging；
Fig. 3 is the tear wax pore fungi aspect graph after solid culture of the present invention, and wherein, Fig. 3 A represents
Cultivate with the fluid medium of sterilizing the liquid-spawn inoculation that obtains to after solid medium through solid culture
The form of the solid culture obtained, Fig. 3 B represents with the fluid medium cultivation acquisition of sterilized
Liquid-spawn inoculation is to the form of the solid culture obtained through solid culture after solid medium；
Fig. 4 is the aspect graph of the degradation material using the tear wax pore fungi of the present invention to obtain.
Detailed description of the invention
Below in conjunction with accompanying drawing, the detailed description of the invention of the present invention is described in detail.It should be appreciated that
Detailed description of the invention described herein is merely to illustrate and explains the present invention, is not limited to this
Bright.
The inoculum concentration that the present invention relates to and bacteria containing amount all with mycelial dry weight (105 DEG C be dried to constant weight time
Weight) meter；The quality of the culture medium that the present invention relates to equally with dry weight (105 DEG C be dried to constant weight time
Weight) meter.
The deposit number of the tear wax pore fungi that the present invention provides is CGMCC No.10485.This tear wax
Pore fungi has the ability of stronger opposing miscellaneous bacteria, fast growth, and the most sterilized available culture medium is carried out
Open cultivation.
The method of the cultivation tear wax pore fungi that the present invention provides includes above-mentioned tear wax pore fungi is seeded to training
Support in base and cultivate.
The tear wax pore fungi of the present invention has the performance of stronger opposing miscellaneous bacteria, it is therefore preferable that in the case of,
Described culture medium is that sterilized or antibacterial process (include that the suppression bacterium of the various routines such as sterilization and sterilization is raw
Long or the mode of breeding) culture medium, and/or the mode of described cultivation is open cultivation (non-sterile formula
Cultivate, i.e. the culture medium used can not be sterilized or antibacterial and directly use, and is also not required to after inoculation
Itself and external environment are sterilely isolated and carries out aseptic culture).Wherein, sterilizing or antibacterial process bag
Include moist heat sterilization, dry heat sterilization, heat sterilization, thermal sterilization, radicidation, interpolation antibacterial and/or antibacterial
Agent and/or the various sterilizing such as antibacterial and/or lysozyme or antibacterial processing mode.
In the present invention, to the condition cultivated not particularly requirement and identical with other funguses, the present invention
Tear wax pore fungi can carry out solid culture, it is also possible to carry out liquid culture.When the tear to the present invention
When wax pore fungi carries out solid culture, the condition of described cultivation preferably includes: temperature is 15-35 DEG C, (cultivates
Environment) relative humidity is 40-95%.When the tear wax pore fungi of the present invention is carried out liquid culture,
The condition of described cultivation includes: temperature is 15-35 DEG C.Additionally, during liquid culture, according to conventional side
Formula (e.g., be passed through compressed air) controls the dissolved oxygen content in cultivating system, usually, dissolves
Oxygen saturation is 5-75%.
The time of described cultivation can carry out suitable selection according to the final purpose of inoculum concentration and cultivation, and one
As, during solid culture, the time of described cultivation can be 5-15 days.Wax hole is torn during solid culture
The inoculum concentration of bacterium can be 1-10g/kg culture medium.During liquid culture, the time of cultivation can be 2-5
My god.
In the present invention, it is required that described culture medium contains the tear wax pore fungi growths such as carbon source, nitrogen source and inorganic salt
Nutrient substance, to the particularly requirement of described culture medium, as long as containing carbonaceous material (such as fiber
Element, starch, hemicellulose, lignin) as carbon source (for solid culture, in terms of C, carbon source
Consumption can be 0.1-0.65g/g culture medium；For liquid culture, in terms of C, the consumption of carbon source is permissible
For 20-45g/L).Under preferable case, described culture medium can also contain wheat bran, Testa oryzae, Semen Maydis
The nitrogenous materials such as slurry, ammonium salt, nitrate, nitrite as nitrogen source (for solid culture, with N
Meter, the consumption in nitrogen source can be 2-15mg/g culture medium；For liquid culture, in terms of N, nitrogen source
Consumption can be 0.1-0.55g/L).During liquid culture, one can also be added as required in the medium
Fixed inorganic salt, such as, (water soluble also provides phosphate anion to phosphate, as selected from biphosphate
At least one in potassium, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate and ammonium dihydrogen phosphate) sulfur
Acid calcium, consumption can be respectively 1-8mg/g culture medium and 5-15mg/g culture medium.
In the present invention, described cultivation can be each stage cultivating tear wax pore fungi, such as activation, seed
Cultivate or (fermentation) stage of use.Those skilled in the art can be easily to various cultivation stage institutes
The culture medium and the condition that use select, and such as, can use PDA culture medium during activation.Activation
Condition can include 20-35 DEG C cultivate 5-10 days.Can use containing following composition during seed culture
Culture medium (by percentage to the quality): water soluble starch 1.5-10%, Semen Maydis pulp 0.5-1% is (with dry weight
Meter), potassium dihydrogen phosphate 0.1-0.8%.The condition of seed culture can include that 15-35 DEG C of cultivation 3-4 days (is
Obtain more seed liquor, liquid seeds training can be carried out in the way of using two-stage liquid seed culture
Support).
In the present invention, either liquid culture or solid culture, all can carry out under non-sterile conditions,
I.e. need not sterile working.And according to the preferred embodiment of the present invention, described cultivation tear wax pore fungi
Method includes being seeded in fluid medium carry out seed culture by the tear wax pore fungi of the present invention, and will obtain
The seed liquor obtained is seeded in solid medium carry out solid culture.Culture medium and actual conditions etc. such as front institute
State, do not repeat them here.
Present invention also offers the application in preparation degradation material of the described tear wax pore fungi.
Use described tear wax pore fungi to prepare degradation material and (tear wax pore fungi is carried out solid foregoing
Body is cultivated) time, described culture medium can be the culture medium containing lignocellulose chip.
Wherein, described lignocellulose chip can derive from plant, such as seed, stem stalk, root, leaf
With at least one in fruit, i.e. can derive from timber, Cotton Gossypii, velveteen, paper, wheat straw, Caulis et Folium Oryzae,
Kaoliang stalk, phragmites communis, fiber crops, Cortex Mori, Folium et Cacumen Broussonetiae Kazinoki, corn stalk, rape straw, Jerusalem artichoke stem stalk, Herba penniseti, thatch
Grass, Miscanthus, grassiness, Jujun grasses, rattan, switchgrass, vine, Caulis Sacchari sinensis and energy-source plant and it
Waste material (i.e. vegetalitas waste material).Described lignocellulose can also derive from microorganism, such as algae
(the raw algae in the most medium-and-large-sized sea, such as Thallus Laminariae (Thallus Eckloniae), Entermorpha) etc..
As it was previously stated, described lignocellulose chip can also be provided by vegetalitas waste material.Described vegetalitas
Waste material can be that the stem and leaf part of crops is (as straw (includes the standing grain such as Oryza sativa L., Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers.
Remaining stem and leaf part after undergraduate course crop maturity threshing), cotton stem, Semen sojae atricolor bar, rape straw, Jerusalem artichoke
Stem stalk, Herba penniseti, Rhizoma Imperatae, Miscanthus, grassiness, Jujun grasses, rattan, switchgrass etc.), seed hulls (as
Cotton seed hulls, rice husk, Pericarppium arachidis hypogaeae, wheat bran, Testa oryzae etc.), wooden waste (wood flour, leftover pieces, fuel wood,
Bark, branch bavin, volume skin, wood shavings etc.), paper scrap, flocking, corn cob, bagasse etc..
It is highly preferred that described vegetalitas waste material is Semen sojae atricolor bar, corn straw, wheat bran, cotton seed hulls, Semen arachidis hypogaeae
At least one in shell, corn cob and leftover pieces.
To the particle diameter of described lignocellulose chip, there is no particular limitation, in order to make degradation material obtain
More preferably molding effect, it is preferable that particle diameter (more preferably at below 25mm, enters one at more than 2mm
Walk preferred 2-15mm) the weight of lignocellulose chip account for lignocellulose chip gross weight
20-100% (such as 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% or
Scope between above-mentioned any two numerical value).
As it is shown in figure 1, it is permissible to utilize the tear wax pore fungi of the present invention to prepare the method for degradable bulk material
It is seeded in the culture medium containing lignocellulose chip enter successively including by the tear wax pore fungi of the present invention
Cultivate in row preculture, mould and cultivate outside mould, being the most optionally dried.Described preculture
Purpose be to increase the Biomass of mycelia.It is certain that the main purpose cultivated in mould makes culture obtain
Three-dimensional shape, is shaped culture by mycelial growth.The purpose cultivated outside described mould is to make bacterium
Filament grows in media surface, improves the intensity of degradation material further, improves outside material simultaneously
See.
In order to pre-incubated above-mentioned purpose is better achieved, it is preferable that relative to the inoculation of tear wax pore fungi
Amount is 1-10g/kg culture medium, and the pre-incubated time is 1-5 days (1,2,3,4,5 or above-mentioned
Anticipate the scope between two numerical value), most preferably 2-4 days.
For the above-mentioned purpose cultivated in mould is better achieved, it is preferable that relative to tear wax pore fungi
Inoculum concentration is 1-10g/kg culture medium, the time cultivated in mould be 1-9 days (1,2,3,4,5,6,
7, the scope between 8,9 or above-mentioned any two numerical value), most preferably 3-6 days.
In order to the above-mentioned purpose cultivated is better achieved outside mould, it is preferable that relative to tear wax pore fungi
Inoculum concentration is 1-10g/kg culture medium, the time cultivated outside mould be 1-5 days (1,2,3,4,5 or
Scope between person's above-mentioned any two numerical value), most preferably 1-3 days.
Material after described preculture is cultivated in generally carrying out mould again after breaing up (such as stirring, rubbing).
The condition cultivated in described preculture, mould and cultivate outside mould may each comprise temperature and is 15-35 DEG C,
(culture environment) relative humidity is 40-95%, and cultivates in preculture, mould and cultivate outside mould
Condition can be identical or different.
After cultivation terminates, the culture obtained is dried, the finished product of degradation material can be obtained.
Described dry mode is preferably vacuum drying, hot air drying, microwave drying, infrared drying or freezing
It is dried.Described dry condition can be conventional drying condition, such as, when the side using hot air drying
When formula is dried, dry condition can include that temperature is 50-150 DEG C, and the time is 0.1-36h.
The density of the degradation material prepared by the tear wax pore fungi of the present invention is 70-400kg/m3, compression
Performance the highest (as in certain embodiment up to 0.5MPa), pressure, degradable, have light weight,
The characteristics such as heat insulation, sound-absorbing, damping；And, the tear wax pore fungi of the application of the invention so that can drop
The preparation technology solving material is simpler.
Hereinafter will be described the present invention by embodiment.
Preparation embodiment 1
Will tear wax pore fungi (deposit number is CGMCC No.10485, hereinafter referred to as YY bacterium) bacterium
Planting and transfer into Kolle flask inclined-plane, culture medium uses PDA culture medium, cultivates 7 days, obtains inclined-plane for 25 DEG C
Strain.
Slant strains accesses first order seed liquid culture medium, and the mass percent formula of this culture medium is:
Soluble starch 2%, Dried Corn Steep Liquor Powder 0.6%, potassium dihydrogen phosphate 0.1%, natural pH, 121 DEG C of sterilizings
20min.The condition cultivated: liquid amount is 150mL/500mL baffle flask, inoculum concentration about 3cm2
Lawn, cultivates 3-4 days in 25 DEG C of shaking table 150rpm, obtains primary seed solution.
Primary seed solution is accessed secondary seed liquid culture medium, the mass percent formula of this culture medium
For: corn starch 6%, Dried Corn Steep Liquor Powder 0.8%, potassium dihydrogen phosphate 0.5%, α-amylase 0.0198%,
PH is natural, 121 DEG C of sterilizing 20min.Volume ratio by 5% is inoculated, liquid amount 150mL/500mL,
Cultivate 3-4 days in 25 DEG C of shaking table 150rpm.The fermentation liquid obtained is (raw as the seed liquor of solid culture
Thing amount dry weight is 5g/L).
Embodiment 1
The present embodiment is used for illustrating the cultural characteristic of the bacterial strain of the present invention.
(1) be respectively adopted sterilizing (composition such as prepares reality with unsterilised secondary seed liquid culture medium
Execute shown in example 1) cultivate (25 DEG C, 150rpm, 7 days) YY bacterium, YY bacterium all can grow, as
Shown in Fig. 2, (Fig. 2 A represents the form after cultivating with the fluid medium of sterilizing, and Fig. 2 B represents with not
The sterilized form after fluid medium cultivation).After the fluid medium using sterilized is cultivated
Hypha body form and culture medium are clearly distinguished from through the nothing of sterilizing, and ullage all has milky bacterium circle, nothing
Microbiological contamination sign, and fermentation liquid abnormal smells from the patient is identical with the YY bacterium cultivated under sterilising conditions.Illustrate that YY bacterium has
Fast growth, the features such as opposing miscellaneous bacteria ability is strong.
(2) by above two fermentation liquid (fluid medium of sterilizing and sterilized in step (1)
Fluid medium cultivate after obtain fermentation liquid) access sterilizing the solid medium (percent mass of composition
Than being cotton seed hulls 79%, wheat bran 20%, Gypsum Fibrosum 1%) in, 25 DEG C of open culturing after 5 days such as Fig. 3 institute
Show that (Fig. 3 A represents that the liquid-spawn inoculation with the fluid medium cultivation acquisition of sterilizing is to solid medium
The form of solid culture (degradation material) obtained by solid culture, Fig. 3 B represent with without
The fluid medium of sterilizing is cultivated the liquid-spawn inoculation obtained and is obtained through solid culture after solid medium
The form of the solid culture (degradation material) obtained), the YY that unsterilised fluid medium is cultivated
The liquid-spawn inoculation of bacterium is to normal growth after solid medium, upgrowth situation and the fluid medium of sterilizing
The liquid-spawn inoculation of the YY bacterium cultivated to the upgrowth situation after solid medium without significant difference.
(3) unsterilised fluid medium is used to carry out open cultivation YY bacterium (condition of culture and step
Suddenly (1) is identical, differ only in employing open cultivation), YY bacterium can normal growth, without microbiological contamination
Sign, and fermentation liquid abnormal smells from the patient is identical with the fermentation liquid of shake-flask culture in step (1).By open cultivation
The fermentation liquid obtained accesses (condition of culture is identical with step (2)), bacterium in solid medium as strain
The normal germination and growth of silk.
Test result indicate that the culture medium that YY bacterium can use sterilized to process is cultivated, and/or institute
Stating cultivation can be open cultivation.
Embodiment 2
The present embodiment is used for the bacterial strain that the present invention is described application in preparation degradation material.
The culture medium that seed liquor preparation embodiment 1 obtained and sterilized process be (medium component
Mass percent is: (in the range of 0.1-15mm, particle diameter is the big of below 2mm to particle diameter to Semen sojae atricolor bar
Bean bar and particle diameter be the weight ratio of the Semen sojae atricolor bar of more than 2mm be 1:1) 99%, Gypsum Fibrosum 1%) mixing,
Inoculum concentration is 4g/kg culture medium, carries out open cultivation at 25 DEG C, and the relative humidity of culture environment is
85%: preculture 3 days so that mycelium the most fully grows, preculture will be cultivated after terminating
Material scattering be placed in mould and carry out cultivating 5 days in mould, after mycelium covers with culture medium, the demoulding
Carry out cultivating 2 days outside mould.It is placed at 55 DEG C and is dried 10h, obtain degradation material A, its aspect graph
As shown in Figure 4 A.
Embodiment 3
The present embodiment is used for the bacterial strain that the present invention is described application in preparation degradation material.
Preparing degradation material B according to the method for embodiment 2, except for the difference that, the particle diameter of Semen sojae atricolor bar is 2
Below mm, the aspect graph of degradation material B is as shown in Figure 4 B.
Embodiment 4-8
These embodiments are used for the bacterial strain that the present invention is described application in preparation degradation material.
Degradation material C-G is prepared, except for the difference that, respectively with " mass ratio according to the method for embodiment 2
Semen sojae atricolor bar and corn straw for 1:1 ", Semen sojae atricolor bar and the cotton seed hulls of 1:1 " mass ratio be ", " mass ratio
Semen sojae atricolor bar and corn cob for 1:1 ", Semen sojae atricolor bar and the Pericarppium arachidis hypogaeae of 1:1 " mass ratio be ", " mass ratio is
The Semen sojae atricolor bar of 1:1 and paper scrap " and replace " Semen sojae atricolor bar ", all energy growth shaping and molding effects are preferable.
Embodiment 9
The present embodiment is used for the bacterial strain that the present invention is described application in preparation degradation material.
The culture medium that seed liquor preparation embodiment 1 obtained and sterilized process be (medium component
Mass percent is: the leftover pieces (particle diameter is in the range of 2-15mm) 99% of willow, Gypsum Fibrosum 1%)
Mixing, inoculum concentration is 10g/kg culture medium, carries out open cultivation (culture environment wet at 35 DEG C
Degree is 65%): preculture 1 day so that mycelium the most fully grows, after preculture terminates
The material scattering of cultivation is placed in mould in carrying out mould and cultivates 3 days, after mycelium covers with culture medium,
The demoulding carries out cultivating 1 day outside mould.Being placed at 65 DEG C and be dried 8h, obtain degradation material H, it becomes
Type effect is substantially the same manner as Example 2.
Embodiment 10
The present embodiment is used for the bacterial strain that the present invention is described application in preparation degradation material.
The culture medium that seed liquor preparation embodiment 1 obtained and sterilized process be (medium component
Mass percent is: (in the range of 0.1-5mm, particle diameter is the Semen Maydis of below 2mm to particle diameter to corn cob
Core and particle diameter be the mass ratio of the corn cob of more than 2mm be 4:1) 99%, Gypsum Fibrosum 1%) mixing, connect
Kind of amount for 2g/kg culture medium, carries out open cultivation (humidity of culture environment is 55%) at 15 DEG C:
Preculture 4 days so that mycelium the most fully grows, the material that preculture will be cultivated after terminating
Breaing up to be placed in mould and carry out the interior cultivation of mould 8 days, after mycelium covers with culture base-material, the demoulding is carried out
Cultivate 3 days outside mould.Be placed at 80 DEG C be dried 20h, obtain degradation material I, its molding effect with
Embodiment 2 is essentially identical.
Testing example 1
This testing example is used for the performance of the degradation material illustrating to utilize the bacterial strain of the present invention to prepare.
By measuring quality and volume, calculate the density of degradation material A-I that embodiment 2-10 prepares；
Degradation material A-I is compressed performance test, and (method of testing sees GB/T 8813-2008
" mensuration of rigid foam compression performance "): use degradation material to need when compressing 50% to apply
Pressure characterize, need apply pressure the biggest, illustrate that the voltage endurance capability of degradation material is the highest；
According in GB/T10294-2008 " adiabator thermal resistance and the mensuration protective plate method about characteristic "
Method, measure degradation material A heat conductivity；
According to the GB/T 18696.1-2010 " measurement of acoustic absorptivity and acoustic resistance the 1st in acoustic impedance pipe
Point: standing-wave ratio method " in method, measure degradation material A noise reduction coefficient；
The measurement result of above-mentioned density, compression performance, heat conductivity and noise reduction coefficient is as shown in table 1 below.
Table 1
| The numbering of degradation material | Density (kg/m3) | Compression performance (MPa) | Heat conductivity (W/ (m K)) | Noise reduction coefficient |
| A | 159±7 | 0.7 | 0.054 | 0.55 |
| B | 162±16 | 0.025 | — | — |
| C | 124±5 | 0.74 | — | — |
| D | 235.4±25 | 1.6 | — | — |
| E | 199.2±5 | 1.8 | — | — |
| F | 199.1±16 | 2.1 | — | — |
| G | 166.1±8 | — | — | — |
| H | 223.6±9 | 2.3 | — | — |
| I | 345.1±23 | 3.5 | — | — |
Note: " " represents and do not test
The degradation material compression performance prepared by the tear wax pore fungi of the present invention is higher, has certain resisting
Compressive Strength；After measured, the degradation material using the tear wax pore fungi of the present invention to obtain also has preferably
Heat-insulating property and sound absorbing capabilities.
The preferred embodiment of the present invention described in detail above, but, the present invention is not limited to above-mentioned reality
Execute the detail in mode, in the technology concept of the present invention, can be to the technical side of the present invention
Case carries out multiple simple variant, and these simple variant belong to protection scope of the present invention.
It is further to note that each the concrete technology described in above-mentioned detailed description of the invention is special
Levy, in the case of reconcilable, can be combined by any suitable means, in order to avoid need not
The repetition wanted, various possible compound modes are illustrated by the present invention the most separately.
Additionally, combination in any can also be carried out between the various different embodiment of the present invention, as long as its
Without prejudice to the thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (8)
1. strain tear wax pore fungi (Ceriporia lacerata), the deposit number of this tear wax pore fungi is
CGMCC No.10485。
2. the method cultivating tear wax pore fungi, it is characterised in that the method includes claim 1
Described tear wax pore fungi is seeded in culture medium cultivate.
Method the most according to claim 2, wherein, described culture medium is sterilized or antibacterial
The culture medium processed.
The most according to the method in claim 2 or 3, wherein, the mode of described cultivation is open
Cultivate.
Method the most according to claim 2, wherein, described cultivation is solid culture, described training
The condition supported includes: temperature is 15-35 DEG C, and relative humidity is 40-95%.
Method the most according to claim 2, wherein, described cultivation is liquid culture, described training
The condition supported includes: temperature is 15-35 DEG C.
7. according to the method described in claim 2,5 or 6, wherein, described method includes wanting right
The tear wax pore fungi described in 1 is asked to be seeded in fluid medium carry out seed culture, and the seed that will obtain
Liquid is seeded in solid medium carry out solid culture.
8. the application in preparation degradation material of the tear wax pore fungi described in claim 1.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164245A (en) * | 2017-07-14 | 2017-09-15 | 西南大学 | One plant tear wax pore fungi growth-promoting functions and its application |
| CN107201317A (en) * | 2017-07-14 | 2017-09-26 | 西南大学 | One plant of tear wax pore fungi and its application for preventing and treating crop fungal disease |
| WO2022068092A1 (en) * | 2020-09-30 | 2022-04-07 | 中国科学院天津工业生物技术研究所 | Use of mycelium material in oil absorption |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925947A (en) * | 2020-08-14 | 2020-11-13 | 四川金珠生态农业科技有限公司 | Preparation method of Ceriporia lacerata spore liquid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101031605B1 (en) * | 2010-11-11 | 2011-04-27 | 김병천 | Manufacturing method of culture extract of ceriporia lacerata for the therapy of diabetic diseases and culture extract of ceriporia lacerata using the same |
| CN102807956A (en) * | 2012-07-12 | 2012-12-05 | 华东理工大学 | Ceriporia lacerata strain and application thereof |
-
2015
- 2015-07-07 CN CN201510395180.2A patent/CN106318876B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101031605B1 (en) * | 2010-11-11 | 2011-04-27 | 김병천 | Manufacturing method of culture extract of ceriporia lacerata for the therapy of diabetic diseases and culture extract of ceriporia lacerata using the same |
| CN102807956A (en) * | 2012-07-12 | 2012-12-05 | 华东理工大学 | Ceriporia lacerata strain and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 图力古尔等: "多孔菌科4种白腐菌培养特性", 《中国食用菌》 * |
| 岳爱玲: "一株撕裂蜡孔菌的分离培养及其系统发育分析", 《生物技术》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164245A (en) * | 2017-07-14 | 2017-09-15 | 西南大学 | One plant tear wax pore fungi growth-promoting functions and its application |
| CN107201317A (en) * | 2017-07-14 | 2017-09-26 | 西南大学 | One plant of tear wax pore fungi and its application for preventing and treating crop fungal disease |
| CN107164245B (en) * | 2017-07-14 | 2020-03-31 | 西南大学 | Growth promoting effect of Ceriporia lacerata and application thereof |
| CN107201317B (en) * | 2017-07-14 | 2020-06-05 | 西南大学 | Ceriporia lacerata and application thereof in preventing and treating crop fungal diseases |
| WO2022068092A1 (en) * | 2020-09-30 | 2022-04-07 | 中国科学院天津工业生物技术研究所 | Use of mycelium material in oil absorption |
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| CN106318876B (en) | 2019-10-11 |
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