CN106318876B - One plant of tearing wax pore fungi and its cultural method and application - Google Patents
One plant of tearing wax pore fungi and its cultural method and application Download PDFInfo
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Abstract
The present invention relates to industrial microorganism fields, disclose one plant of tearing wax pore fungi, and the deposit number of the tearing wax pore fungi is CGMCC No.10485.The invention also discloses a kind of methods for cultivating the tearing wax pore fungi, and this method includes that the tearing wax pore fungi is seeded in culture medium to cultivate.In addition, the application the invention discloses the tearing wax pore fungi in preparation degradation material.The mycelium that the present invention tears wax pore fungi can wind the clast of fixed vegetalitas culture medium material, by tearing wax pore fungi of the invention, vegetalitas material can prepare degradation material in the case where not sterilized or antibacterial processing, and preparation process does not require gnotobasis, and the ability for resisting miscellaneous bacteria is strong；Since the ability of the resistance miscellaneous bacteria of tearing wax pore fungi of the invention is strong, various culture (Liquid Culture or solid culture) processes can carry out under conditions of non-sterile, so can largely reduce the preparation cost of degradation material.
Description
Technical field
The invention belongs to industrial microorganism fields, are related to one plant of tearing wax pore fungi (Ceriporia lacerata) and its training
Support methods and applications.
Background technique
Foamed plastics is common heat preservation, heat-insulated and padded coaming, multi-field in building, packaging, filling, energy absorption etc.
It is widely used.But foamed plastics faces two hang-ups: first is that raw material comes from petroleum, such as every production 1m3Polystyrene bubble
Foam plastics need 65L petroleum, and petroleum is limited, and the dependence on external supply degree of China's oil has been more than 60%, seek petroleum base
The sub of foamed plastics is imperative；Second is that being difficult to be decomposed in the natural environment, ecological environment and life cycle are seriously endangered
Border.The plastic refuse to drift about in rivers and lakes and the plastic refuse being mingled in herbage oneself prestige is caused to fish and livestock
The side of body.For example, there is the animals such as a large amount of seabird dead because eating discarded plastics every year.According to the California seashore committee
The investigation of (California Coastal Commission), foamed plastics have been main ocean drifts, and to eating this by mistake
For the marine organisms of class plastic cement, its digestive system can be damaged, but there is presently no any of organisms can
It degrades to it.Plastic garbage not only influences environmental beauty, but also polluted source and soil, jeopardizes poultry and wild animal,
Heavy burden is brought to ball ecological environment.The main approaches and methods that China handles municipal refuse are exactly to fill.
On the one hand since foam plastic density is small, volume is big, selected place can be filled up quickly by it, handle rubbish with causing landfill yard
Ability is greatly reduced, and causes biggish pollution to environment；On the other hand, after place is all filled, comparatively ground just becomes
Must be especially soft, the harmful substances such as bacterium, virus in rubbish are easy to be impregnated into underground, and polluted underground water jeopardizes environment；Modeling
When material burns, dioxin can be generated, serious secondary pollution will be caused to environment by burning foam plastic waste.Since foreign countries are to bubble
The resistance of foam plastics, the foamed plastics padded coaming that product packaging uses have seriously affected the outlet of some products in China.
To solve the above problems, the research of biology base degradation plastic is in the ascendant, but bio-based plastics so far, it is such as poly-
Lactic acid, PBS, PPC, biology PP, biology base PET, biological poly amide and biological poly ethylene etc., be using sugar as raw material, with
People and animals strive grain；Even also first had to for fermentation raw material by lignocellulosic water with lignocellulosic (lignocellulose)
Solution is sugar, then by liquid deep layer fermenting or is biologically converted into the monomer of synthetic plastic (PHA is to utilize the direct biosynthesis of sugar
High molecular material, accumulated in the cell after synthesis, therefore have to pass through technology for broken wall, thick PHA could be obtained), then with chemistry side
Method is polymerized to bio-based plastics.The consumption of such technique, raw material and the energy is big, and process route is long, and production technology is complicated, equipment
Investment is big, and product cost is high, and synthesis high molecular degradable plastics cost is higher than existing general-purpose plastics, production process pollution compared with
Weight.Therefore, this kind of product is still difficult to popularize at this stage.
More mature starch plastic is flourished from the eighties in last century, and U.S.'s annual growth is once up to 75%, when
When mainly filled-type starch-based degradable plastics.Starch-based degradable plastics are one kind biologies for developing so far at most
Degradative plastics, there are many research unit both domestic and external, and only the research unit in China just has 40 or more.Successively from the 1990s
The result of study delivered shows that they only degrade starch therein, and other components only broken into pieces is still in soil and waters
Among, it can not fundamentally solve " white pollution " of plastics.
For terrestrial plant in long-term evolutionary process, foring by cellulose, hemicellulose and lignin is main component
Compact structure and complexity cell wall, lignin and hemi-cellulose components knot are tightly wrapped cellulose polysaccharide portion together
Point, the mixture of this three-dimensional structure is referred to as lignocellulosic.Lignocellulosic is the maximum amount of renewable biomass, estimation
Global annual output is up to 1 × 1010MT(Sánchez,ó.J.,Cardona,C.A.,2008.Trends in
biotechnological production of fuel ethanol from different
Feedstocks.Bioresour.Technol.99,5270-5295.), it is the ideal resource of human future.Currently, China is every
Year has the reproducible lignocellulosic sources such as up to hundred million tons of 6-7 of waste straw not developed and used economically and effectively,
Even become the burden in rural area and peasant, crop straw burning remains incessant after repeated prohibition, generates a large amount of PM 2.5, become the new pollution in China
Source.How really to convert the stalk of such flood tide to the resource that can create value, has become Chinese society economic development
Strategic issue.
If can be using stalk resource as raw material, with novel fermentation technology low cost, contamination-freely manufacturing market capacity be big, passes through
Help profitable, environmental-friendly bio-based materials, will adjust to Industry Structure, or even has weight to the development of economy and society
Want meaning.
Although at present there are also about the report for preparing bio-based materials using microorganism, the liquid being related to
Incubation and/or solid culture process are required to aseptically carry out, and require condition of culture harsh, bio-based materials
Preparation cost it is high.
Summary of the invention
It can be used for preparing mycelium material and the tearing low to condition of culture requirement the object of the present invention is to provide a kind of
Wax pore fungi and its cultural method and application.
To achieve the goals above, the present inventor has carried out many experiments, and screening one plant can effectively wind
Fixed vegetalitas material prepares the tearing wax pore fungi of degradable bulk material.Therefore, in a first aspect, the present invention provides one kind to tear
It splits wax pore fungi (Ceriporia lacerata), the deposit number of the tearing wax pore fungi is CGMCC No.10485.
Second aspect, the present invention provides a kind of method that wax pore fungi is torn described in culture first aspect, this method packets
It includes for tearing wax pore fungi described in first aspect to be seeded in culture medium and cultivate.
The third aspect, the present invention provides the answering in preparation degradation material of the tearing wax pore fungi described in first aspect
With.
The mycelium that the present invention tears wax pore fungi can wind the clast of fixed vegetalitas culture medium material, by the present invention
Tearing wax pore fungi, vegetalitas material can prepare degradation material in the case where not sterilized or antibacterial processing, and prepare
Journey does not require gnotobasis, and the ability for resisting miscellaneous bacteria is strong.Since the ability of the resistance miscellaneous bacteria of tearing wax pore fungi of the invention is strong,
Various culture (Liquid Culture or solid culture) processes can carry out under conditions of non-sterile, so can be largely
The upper preparation cost for reducing degradation material.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Biological deposits
It is micro- to be deposited in China on April 28th, 2015 to tearing wax pore fungi (Ceriporia lacerata) of the invention
Biological inoculum preservation administration committee common micro-organisms center (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese section
Institute of microbiology, institute, postcode: 100101) (depositary institution is abbreviated as CGMCC), deposit number CGMCC
No.10485。
Detailed description of the invention
The drawings are intended to provide a further understanding of the invention, and constitutes part of specification, with following tool
Body embodiment is used to explain the present invention together, but is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is the flow chart using a kind of embodiment of tearing wax pore fungi preparation degradation material method of the invention；
Fig. 2 is the aspect graph of tearing wax pore fungi of the invention after Liquid Culture, wherein Fig. 2A indicates the liquid with sterilizing
Form after culture medium culture, Fig. 2 B indicate the form after non-sterilized fluid nutrient medium culture；
Fig. 3 is the aspect graph of tearing wax pore fungi of the invention after solid culture, wherein Fig. 3 A indicates the liquid with sterilizing
The liquid spawn that culture medium culture obtains is inoculated into the form of the solid culture obtained after solid medium through solid culture, figure
3B indicates that the liquid spawn obtained with non-sterilized fluid nutrient medium culture is inoculated into after solid medium and obtains through solid culture
The form of the solid culture obtained；
Fig. 4 is the aspect graph of the degradation material obtained using tearing wax pore fungi of the invention.
Specific embodiment
Below in conjunction with attached drawing, detailed description of the preferred embodiments.It should be understood that this place is retouched
The specific embodiment stated is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
Inoculum concentration of the present invention and bacteria containing amount are with mycelial dry weight (weight when 105 DEG C of drying to constant weights)
Meter；The quality of culture medium of the present invention is equally in terms of dry weight (weight when 105 DEG C of drying to constant weights).
The deposit number of tearing wax pore fungi provided by the invention is CGMCC No.10485.The tearing wax pore fungi has relatively strong
Resistance miscellaneous bacteria ability, the speed of growth is fast, can carry out open culture with not sterilized culture medium.
It is provided by the invention culture tearing wax pore fungi method include above-mentioned tearing wax pore fungi is seeded in culture medium into
Row culture.
Tearing wax pore fungi of the invention has the stronger performance for resisting miscellaneous bacteria, it is therefore preferable that in the case of, the culture medium
For the culture of non-sterilized or antibacterial processing (including the various conventional modes for inhibiting bacterium growth or breeding such as sterilizing and sterilizing)
Base and/or the mode of the culture are open culture (non-sterile formula culture, that is, used culture medium can not be sterilized
Or it is antibacterial and directly use, also do not need for it to be sterilely isolated with external environment after inoculation and carry out sterile culture).Wherein,
Sterilizing or antibacterial processing include moist heat sterilization, hot air sterilization, heat sterilization, thermal sterilization, radio sterilization, addition fungicide and/or suppression
The various sterilizings such as microbial inoculum and/or antibacterial agent and/or lysozyme or antibacterial processing mode.
In the present invention, the condition of culture is not required particularly, and identical as other fungies, tearing wax hole of the invention
Bacterium can carry out solid culture, can also carry out Liquid Culture.When carrying out solid culture to tearing wax pore fungi of the invention, institute
The condition for stating culture preferably includes: temperature is 15-35 DEG C, and (culture environment) relative humidity is 40-95%.When to of the invention
When tearing wax pore fungi progress Liquid Culture, the conditions for the training include: temperature is 15-35 DEG C.In addition, being pressed when Liquid Culture
Dissolved oxygen content in mode (e.g., being passed through compressed air) the control cultivating system of more solito generally dissolves oxygen saturation
Degree is 5-75%.
The time of the culture can make appropriate choice according to inoculum concentration and the final purpose of culture, generally, Gu
When body culture, the time of the culture can be 5-15 days.It can be 1-10g/ that the inoculum concentration of wax pore fungi is torn when solid culture
Kg culture medium.When Liquid Culture, the time of culture can be 2-5 days.
In the present invention, nutrients needed for the culture medium contains the tearing wax pore fungi growth such as carbon source, nitrogen source and inorganic salts
Matter does not require the culture medium particularly, as long as (such as cellulose, starch, hemicellulose, wooden containing carbonaceous material
Element) as carbon source, (for solid culture, in terms of C, the dosage of carbon source can be 0.1-0.65g/g culture medium；Liquid is trained
It supports, in terms of C, the dosage of carbon source can be 20-45g/L).Under preferable case, the culture medium can also contain wheat bran, rice
The nitrogenous material such as chaff, corn pulp, ammonium salt, nitrate, nitrite is as nitrogen source (for solid culture, in terms of N, nitrogen source
Dosage can be 2-15mg/g culture medium；For Liquid Culture, in terms of N, the dosage of nitrogen source can be 0.1-0.55g/L).Liquid
When culture, certain inorganic salts can also be added in the medium as needed, it is (water-soluble and phosphorus is provided for example, phosphate
Acid ion, such as in potassium dihydrogen phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate and ammonium dihydrogen phosphate extremely
Few one kind) calcium sulfate, dosage can be respectively 1-8mg/g culture medium and 5-15mg/g culture medium.
In the present invention, the culture can be each stage of culture tearing wax pore fungi, such as activation, seed culture or use
(fermentation) stage.Those skilled in the art can easily select culture medium used in various cultivation stages and condition
It selects, for example, PDA culture medium can be used when activation.The condition of activation may include 20-35 DEG C of culture 5-10 days.Seed culture
When the culture medium (by percentage to the quality) containing following ingredient: water soluble starch 1.5-10%, corn pulp 0.5- can be used
1% (in terms of dry weight), potassium dihydrogen phosphate 0.1-0.8%.The condition of seed culture may include 15-35 DEG C of culture 3-4 days (in order to
More seed liquors are obtained, liquid seeds culture can be carried out by the way of two-stage liquid seed culture).
In the present invention, either Liquid Culture or solid culture can be carried out under non-sterile conditions, that is, not needed
Sterile working.And according to the preferred embodiment of the present invention, the method for the culture tearing wax pore fungi includes tearing of the invention
It splits wax pore fungi and is seeded in fluid nutrient medium and carry out seed culture, and the seed liquor of acquisition is seeded in solid medium and is carried out
Solid culture.Culture medium and actual conditions etc. are as previously mentioned, details are not described herein.
The present invention also provides application of the tearing wax pore fungi in preparation degradation material.
It uses tearing wax pore fungi preparation degradation material (tearing wax pore fungi is subjected to foregoing solid culture)
When, the culture medium can be the culture medium containing lignocellulosic clast.
Wherein, the lignocellulosic clast can be in plant, such as seed, stalk, root, leaf and fruit extremely
Few one kind, that is, timber, cotton, velveteen, paper, wheat straw, straw, kaoliang stalk, reed, fiber crops, mulberry skin, paper mulberry skin, corn can be derived from
Straw, rape straw, jerusalem artichoke stalk, Chinese pennisetum, cogongrass, Chinese silvergrass, napier grass, Jujun grasses, rattan, switchgrass, grapevine, sugarcane and the energy
Plant and their waste material (i.e. vegetalitas waste material).The lignocellulosic can also derive from microorganism, (especially such as algae
It is the medium-and-large-sized raw algae in sea, such as kelp, Enteromorpha) etc..
As previously mentioned, the lignocellulosic clast can also be provided by vegetalitas waste material.The vegetalitas waste material can be with
For the stem and leaf parts of crops, (such as stalk is (including remaining after rice, wheat, corn, sorghum gramineae farm crop maturation threshing
Stem and leaf part), cotton stem, soybean bar, rape straw, jerusalem artichoke stalk, Chinese pennisetum, cogongrass, Chinese silvergrass, napier grass, Jujun grasses, rattan,
Switchgrass etc.), seed hulls (such as cotton seed hulls, rice husk, peanut shell, wheat bran, rice bran), wooden waste (sawdust, leftover pieces, fuel wood,
Bark, branch bavin, volume skin, wood shavings etc.), paper scrap, flocking, corncob, bagasse etc..
It is highly preferred that the vegetalitas waste material be soybean bar, corn stover, wheat bran, cotton seed hulls, peanut shell, corncob and
At least one of leftover pieces.
To the partial size of the lignocellulosic clast, there is no particular limitation, in order to make degradation material obtain more preferably at
Type effect, it is preferable that partial size is broken in the lignocellulosic of 2mm or more (more preferably in 25mm hereinafter, further preferred 2-15mm)
The weight of bits account for lignocellulosic clast total weight 20-100% (such as 20%, 30%, 40%, 50%, 60%, 70%,
80%, the range between 90%, 100% or above-mentioned any two numerical value).
As shown in Figure 1, may include by this using the method that tearing wax pore fungi of the invention prepares degradable bulk material
The tearing wax pore fungi of invention be seeded in the culture medium containing lignocellulosic clast and successively carry out preculture, cultivate in mold and
It cultivates outside mold, is then selectively dried.The purpose of the preculture is the biomass for increasing mycelia.Culture in mold
Main purpose be that culture is made to obtain certain three-dimensional shape, culture is formed by mycelial growth.The mold
The purpose of outer culture is to grow mycelium in media surface, further increases the intensity of degradation material, while improving material
The appearance of material.
In order to which the above-mentioned purpose of preculture is better achieved, it is preferable that the inoculum concentration relative to tearing wax pore fungi is 1-
10g/kg culture medium, the time of preculture are 1-5 days (range between 1,2,3,4,5 or above-mentioned any two numerical value), most
Preferably 2-4 days.
In order to which the above-mentioned purpose cultivated in mold is better achieved, it is preferable that be relative to the inoculum concentration for tearing wax pore fungi
1-10g/kg culture medium, the mold interior time cultivated is 1-9 days (1,2,3,4,5,6,7,8,9 or above-mentioned any two numerical value
Between range), most preferably 3-6 days.
In order to which the above-mentioned purpose cultivated outside mold is better achieved, it is preferable that be relative to the inoculum concentration for tearing wax pore fungi
1-10g/kg culture medium, the time that mold is cultivated outside are the 1-5 days (models between 1,2,3,4,5 or above-mentioned any two numerical value
Enclose), most preferably 1-3 days.
Material after the preculture carries out culture in mold after usually being broken up (such as stirring, rubbing) again.The pre- training
It is 15-35 DEG C that the condition support, cultivated outside the interior culture of mold and mold, which may each comprise temperature, and (culture environment) relative humidity is
40-95%, and the condition that culture and mold are cultivated outside in preculture, mold can be identical or different.
After culture, obtained culture is dried, can be obtained the finished product of degradation material.The drying
Mode is preferably vacuum drying, heated-air drying, microwave drying, infrared drying or freeze-drying.The condition of the drying can be with
For conventional drying condition, for example, dry condition may include that temperature is when being dried by the way of heated-air drying
50-150 DEG C, time 0.1-36h.
The density of the degradation material as made from tearing wax pore fungi of the invention is 70-400kg/m3, compression performance also compared with
It is high that (up to 0.5MPa) such as in certain embodiment, pressure resistance is degradable, has the characteristics such as light, heat-insulated, sound-absorbing, damping；And
And by using tearing wax pore fungi of the invention, so that the preparation process of degradation material is simpler.
The present invention will be described in detail by way of examples below.
Prepare embodiment 1
It is oblique that tearing wax pore fungi (deposit number is CGMCC No.10485, hereinafter referred to as YY bacterium) strain transfer is entered into Kolle flask
Face, culture medium use PDA culture medium, and 25 DEG C are cultivated 7 days, obtain slant strains.
Slant strains are accessed into first order seed liquid culture medium, the mass percent formula of the culture medium are as follows: solubility is formed sediment
Powder 2%, Dried Corn Steep Liquor Powder 0.6%, potassium dihydrogen phosphate 0.1%, natural pH, 121 DEG C of sterilizing 20min.The condition of culture: liquid amount
For 150mL/500mL baffle flask, inoculum concentration about 3cm2Lawn is cultivated 3-4 days in 25 DEG C of shaking table 150rpm, obtains level-one kind
Sub- liquid.
Primary seed solution is accessed into secondary seed liquid culture medium, the mass percent formula of the culture medium are as follows: corn forms sediment
Powder 6%, Dried Corn Steep Liquor Powder 0.8%, potassium dihydrogen phosphate 0.5%, alpha-amylase 0.0198%, pH is naturally, 121 DEG C of sterilizing 20min.
It is inoculated with by 5% volume ratio, liquid amount 150mL/500mL is cultivated 3-4 days in 25 DEG C of shaking table 150rpm.The fermentation liquid of acquisition is made
For the seed liquor (biomass dry weight 5g/L) of solid culture.
Embodiment 1
The present embodiment is used to illustrate the cultural characteristic of bacterial strain of the invention.
(1) sterilizing and unsterilised secondary seed liquid culture medium is respectively adopted (ingredient is as shown in preparation embodiment 1)
(25 DEG C, 150rpm, 7 days) YY bacterium is cultivated, YY bacterium can grow, and (Fig. 2A indicates the fluid nutrient medium with sterilizing as shown in Figure 2
Form after culture, Fig. 2 B indicate the form after non-sterilized fluid nutrient medium culture).It is trained using non-sterilized liquid
By sterilizing without significant difference, ullage has milky bacterium circle, nothing for hypha body form and culture medium after supporting base culture
Microbiological contamination sign, and the YY bacterium cultivated under fermentation liquid smell and sterilising conditions is identical.Illustrate that YY bacterium has the speed of growth fast, resists miscellaneous
The features such as bacterium ability is strong.
(2) by above two fermentation liquid, (fluid nutrient medium and non-sterilized fluid nutrient medium to sterilize in step (1) is trained
The fermentation liquid that obtains after supporting) access sterilizing solid medium (mass percent of ingredient is cotton seed hulls 79%, wheat bran 20%,
Gypsum 1%) in, 25 DEG C (Fig. 3 A indicates the liquid obtained with the fluid nutrient medium culture of sterilizing as shown in Figure 3 after open culturing 5 days
Body strain is inoculated into the form of the solid culture (degradation material) obtained after solid medium through solid culture, and Fig. 3 B is indicated
Consolidate after being inoculated into solid medium with the liquid spawn that non-sterilized fluid nutrient medium culture obtains through what solid culture obtained
The form of body culture (degradation material)), the liquid spawn of the YY bacterium of unsterilised fluid nutrient medium culture is inoculated into solid
The liquid spawn of the YY bacterium of the fluid nutrient medium culture of normal growth after culture medium, upgrowth situation and sterilizing is inoculated into solid culture
Upgrowth situation after base is without significant difference.
(3) using the unsterilised open culture YY bacterium of fluid nutrient medium progress, (condition of culture is identical as step (1), area
Be not only that using open culture), YY bacterium can normal growth, no microbiological contamination sign, and being shaken in fermentation liquid smell and step (1)
The fermentation liquid of bottle culture is identical.Using open obtained fermentation liquid of cultivating as (condition of culture in strain access solid medium
It is identical as step (2)), the normal germination and growth of mycelia.
The experimental results showed that YY bacterium can be cultivated using the culture medium of non-sterilized processing and/or the culture can
Think open culture.
Embodiment 2
The present embodiment is used to illustrate the application of bacterial strain of the invention in preparation degradation material.
Culture medium (the quality percentage of medium component of seed liquor and non-sterilized processing that embodiment 1 obtains will be prepared
Than are as follows: (for partial size within the scope of 0.1-15mm, partial size is the soybean bar that 2mm soybean bar below is 2mm or more with partial size to soybean bar
Weight ratio be 1:1) 99%, gypsum 1%) mixing, inoculum concentration be 4g/kg culture medium, open culture is carried out at 25 DEG C, train
The relative humidity for supporting environment is 85%: preculture 3 days, so that mycelium is sufficiently grown in the medium, it will after preculture
The material scattering of culture, which is placed in mold, carries out culture 5 days in mold, and after mycelium covers with culture medium, demoulding is carried out outside mold
Culture 2 days.It is placed at 55 DEG C dry 10h, obtains degradation material A, aspect graph is as shown in Figure 4 A.
Embodiment 3
The present embodiment is used to illustrate the application of bacterial strain of the invention in preparation degradation material.
Degradation material B is prepared according to the method for embodiment 2, unlike, the partial size of soybean bar is 2mm hereinafter, can drop
The aspect graph for solving material B is as shown in Figure 4 B.
Embodiment 4-8
These embodiments are used to illustrate the application of bacterial strain of the invention in preparation degradation material.
Degradation material C-G is prepared according to the method for embodiment 2, unlike, respectively with " mass ratio is the soybean of 1:1
Bar and corn stover ", " soybean bar and cotton seed hulls that mass ratio is 1:1 ", " soybean bar and corncob that mass ratio is 1:1 ", " matter
Amount is than the soybean bar and peanut shell by 1:1 ", " soybean bar and paper scrap of the mass ratio by 1:1 " and replace " soybean bar ", can grow
It forms and molding effect is preferable.
Embodiment 9
The present embodiment is used to illustrate the application of bacterial strain of the invention in preparation degradation material.
Culture medium (the quality percentage of medium component of seed liquor and non-sterilized processing that embodiment 1 obtains will be prepared
Than are as follows: the leftover pieces (partial size is within the scope of 2-15mm) 99% of poplar, gypsum 1%) mixing, inoculum concentration is 10g/kg culture medium,
It is carried out at 35 DEG C open culture (humidity of culture environment is 65%): preculture 1 day, so that mycelium fills in the medium
The material scattering of culture is placed in mold after preculture and cultivate 3 days in mold, covers with training to mycelium by mitogenetic length
After supporting base, demoulding cultivate 1 day outside mold.It is placed at 65 DEG C dry 8h, obtains degradation material H, molding effect and reality
It is essentially identical to apply example 2.
Embodiment 10
The present embodiment is used to illustrate the application of bacterial strain of the invention in preparation degradation material.
Culture medium (the quality percentage of medium component of seed liquor and non-sterilized processing that embodiment 1 obtains will be prepared
Than are as follows: (for partial size within the scope of 0.1-5mm, partial size is the corncob that 2mm corncob below is 2mm or more with partial size to corncob
Mass ratio be 4:1) 99%, gypsum 1%) mixing, inoculum concentration be 2g/kg culture medium, carried out at 15 DEG C it is open culture (training
55%) humidity for supporting environment is: preculture 4 days, so that mycelium is sufficiently grown in the medium, it will culture after preculture
Material scattering be placed in mold carry out mold in culture 8 days, after mycelium covers with culture base-material, demoulding carry out mold outside train
It supports 3 days.It is placed at 80 DEG C dry 20h, obtains degradation material I, molding effect is substantially the same manner as Example 2.
Testing example 1
This testing example is used to illustrate the performance of the degradation material prepared using bacterial strain of the invention.
By measurement quality and volume, the density of degradation material A-I made from embodiment 2-10 is calculated；
Degradation material A-I is carried out compression performance test, and (test method referring to GB/T 8813-2008, " mould by rigid foam
Expect the measurement of compression performance "): it needs the pressure applied to be characterized when using degradation material compression 50%, needs to apply
Pressure is bigger, illustrates that the voltage endurance capability of degradation material is higher；
According to the method in GB/T10294-2008 " heat-insulating material thermal resistance and measurement protective plate method " in relation to characteristic, measurement
The thermal coefficient of degradation material A；
According to GB/T 18696.1-2010 " the measurement part 1 of acoustic absorptivity and acoustic resistance in acoustic impedance pipe: standing-wave ratio
Method " in method, measure degradation material A noise reduction coefficient；
Above-mentioned density, compression performance, the measurement result of thermal coefficient and noise reduction coefficient are as shown in table 1 below.
Table 1
| The number of degradation material | Density (kg/m3) | Compression performance (MPa) | Thermal coefficient (W/ (mK)) | Noise reduction coefficient |
| A | 159±7 | 0.7 | 0.054 | 0.55 |
| B | 162±16 | 0.025 | — | — |
| C | 124±5 | 0.74 | — | — |
| D | 235.4±25 | 1.6 | — | — |
| E | 199.2±5 | 1.8 | — | — |
| F | 199.1±16 | 2.1 | — | — |
| G | 166.1±8 | — | — | — |
| H | 223.6±9 | 2.3 | — | — |
| I | 345.1±23 | 3.5 | — | — |
Note: "-" representative is not tested
The degradation material compression performance as made from tearing wax pore fungi of the invention is higher, has certain compression strength；
After measured, also there is preferable thermal insulation property and sound absorbing performance using the degradation material that tearing wax pore fungi of the invention obtains.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (8)
1. one plant of tearing wax pore fungi (Ceriporia lacerata), the deposit number of the tearing wax pore fungi is CGMCC
No.10485。
2. a kind of method of culture tearing wax pore fungi, which is characterized in that this method includes by tearing wax hole described in claim 1
Bacterium is seeded in culture medium and is cultivated.
3. according to the method described in claim 2, wherein, the culture medium is the culture medium of non-sterilized or antibacterial processing.
4. according to the method in claim 2 or 3, wherein the mode of the culture is open culture.
5. the culture is solid culture according to the method described in claim 2, wherein, the conditions for the training include: temperature
It is 15-35 DEG C, relative humidity 40-95%.
6. the culture is Liquid Culture according to the method described in claim 2, wherein, the conditions for the training include: temperature
It is 15-35 DEG C.
7. according to method described in claim 2,5 or 6, wherein the method includes by tearing wax hole described in claim 1
Bacterium, which is seeded in fluid nutrient medium, carries out seed culture, and the seed liquor of acquisition is seeded to progress solid training in solid medium
It supports.
8. application of the tearing wax pore fungi described in claim 1 in preparation degradation material.
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| CN107164245B (en) * | 2017-07-14 | 2020-03-31 | 西南大学 | Growth promoting effect of Ceriporia lacerata and application thereof |
| CN112225326A (en) * | 2020-09-30 | 2021-01-15 | 中国科学院天津工业生物技术研究所 | Application of mycelium material in oil absorption |
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