CN106309346B - Compound skin external preparation and preparation method and application containing water shield - Google Patents
Compound skin external preparation and preparation method and application containing water shield Download PDFInfo
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Abstract
The invention discloses a kind of preparation for external application to skin feedstock compositions, and the composition is by weight, comprising: 10-90 parts of water shield powder, 10-50 parts of Radix Glycyrrhizae, 5-90 parts of tremella.A kind of preparation for external application to skin stoste is also disclosed in the present invention, and the stoste is by weight, comprising: water shield extracting solution 10-90,10-50 parts of licorice extract, 5-90 parts of tremella extract.The method for preparing the preparation for external application to skin stoste is also disclosed in the present invention, this method is to be placed in water water shield powder, licorice powder, tremella powder, prior to 60 DEG C heat preservation 1-2 hours, it extracts again 0.5-2 hours, when extraction, temperature be not less than 60 DEG C, by extract carry out centrifuging and taking supernatant and filter, cross polyamide column purifying, concentration.The present invention is also disclosed the stoste in the application for preparing preparation for external application to skin.Raw material of the present invention is easy to get, is cheap；Performance of keeping humidity, antioxygenic property, removing free radical performance are very outstanding；Preparation process is simple, low production cost；Without organic solvent, pigment tanning.
Description
Technical field
The invention belongs to preparation for external application to skin fields, and in particular to a kind of compound skin external preparation raw material containing water shield,
Stoste and its preparation method and application.
Background technique
When whether evaluation preparation for external application to skin (especially cosmetic formulations) is excellent, in addition to humidity-preserving type, such as antioxygen
The property changed and removing free radical performance are also the important indicator of evaluation.The preparation for external application to skin for having both multiple excellent efficacy is research
The important research content of personnel.
Existing preparation for external application to skin generally all contains a small amount of organic solvent (such as ethyl alcohol), although the use side of organic solvent
The extraction of effective component (such as antioxidant and antifree radical agent) in preparation, but also result in high production cost enterprise and can
Skin can be adversely affected.
Since many medicinal and edible plants are smaller to skin damage, always using medicinal and edible plant as preparation for external application to skin
It is concerned by people.However, since (1) various medicinal and edible plants are used to prepare the active constituent content difference of preparation for external application to skin very
Greatly；(2) medicinal and edible plant ingredient is more, and it is larger to extract difficulty；(3) medicinal and edible plant is easy corruption, and ingredient is not easily-controllable
System；Therefore, suitable medicinal and edible plant how is selected, stablizes its ingredient and constitutes and efficiently extract its effective component and be made
Excellent preparation for external application to skin, becomes urgent problem to be solved.
Chinese medicine is the rarity of the Chinese nation, is applied to preparation for external application to skin, especially cosmetic formulations, is ability
The pursuit of domain researcher for many years.However, since external preparation for skin product has particular/special requirement, such as humidity-preserving type, inoxidizability, no
Irritation etc., and it is not yet very deep to the related development of Chinese herbal medicine, cause the application of Chinese herbal medicine to be restricted bad
Status.
Therefore, medicinal and edible plant and Chinese herbal medicine how to be selected to carry out compounding excellent preparation for external application to skin is made being one
The research of great challenge.
Summary of the invention
In view of the shortcomings of the prior art with this field urgent need to resolve the problem of, one of the objects of the present invention is to provide one kind
Preparation for external application to skin feedstock composition, the composition is by weight, comprising:
10-90 parts of water shield powder
10-50 parts of Radix Glycyrrhizae
5-90 parts of tremella；
The water shield powder is that fresh water shield is spray-dried gained after homogenized；
The preparation for external application to skin includes facial mask, Essence, lotion, emulsifiable paste, gel preparation.
Preferably, the composition is by weight, comprising:
30-60 parts of water shield powder
10-30 parts of Radix Glycyrrhizae
20-60 parts of tremella.
Preferably, the water shield powder is that volume is added and is no more than the pure of volume shared by water shield leaf after cleaning fresh water shield
Water handles 30 min using refiner under 4000rpm, be spray-dried at 60 DEG C obtained by.
It is another object of the present invention to provide a kind of preparation for external application to skin stostes, and the stoste is by weight, comprising:
10-90 parts of water shield extracting solution
10-50 parts of licorice extract
5-90 parts of tremella extract；
In the water shield extracting solution, polyoses content is at least 0.6 mg/ml, and water shield total polyphenols are at least 1.2 × 10-4mg/
Ml is free of pigment tanning and organic solvent；Glycyrrhizic acid is at least 2.08 × 10 in the licorice extract-3mg/ml；The tremella
Tremella polysaccharides are at least 2.5 × 10 in extracting solution-3mg/ml。
As shown in the embodiment of present invention, stoste of the present invention has very outstanding humidity-preserving type, than existing outstanding commercialization
The performance of keeping humidity of product is more outstanding and has very significant inoxidizability and removes the ability of free radical.
Tremella polysaccharides and Polysaccharide of Brasenia Schreberi are used cooperatively, and the synergistic effect of polysaccharide enhances its skin-moisturizing, skin effect, are provided simultaneously
More nutrition, the addition of tremella, so that skin preparation quality obtained is more preferable, the enhancing of skin affinity, viscosity and transparency increase
Add, moistening effect increases.Radix Glycyrrhizae plays anti-inflammatory whitening function, and with water shield compatible use, anti-inflammatory antioxidant activity enhancing is had both
Mild whitening and skin-protecting effect.Radix Glycyrrhizae and tremella are all warm nature, and sweet in flavor nontoxic, water shield is cold in nature sweet in flavor nontoxic, throws after three's compatibility
Doses is reduced, and skin care effect is strengthened, and is avoided extract concentrations height from influencing the quality of product, is achieved the desired results simultaneously.
Preferably, the stoste is by weight, comprising:
30-60 parts of water shield extracting solution
10-30 parts of licorice extract
20-60 parts of tremella extract.
It is another object of the present invention to provide the methods for preparing above-mentioned stoste, and this method comprises the following steps:
(1) prepared by water shield powder: fresh water shield being homogenized and is spray-dried again, water shield powder is obtained, is stored；
(2) water shield powder obtained by licorice powder, tremella powder, step (1) is extracted with water respectively, obtains Radix Glycyrrhizae respectively and extracts
Liquid, tremella extract, water shield extracting solution；It controls in water shield extracting solution, polyoses content is at least 0.6 mg/ml, water shield total polyphenols
At least 1.2 × 10-4Mg/ml is free of pigment tanning and organic solvent；In control licorice extract glycyrrhizic acid be at least 2.08 ×
10-3mg/ml；Tremella polysaccharides are at least 2.5 × 10 in control tremella extract-3mg/ml；By weight, by water shield extracting solution
10-90 parts, 10-50 parts of licorice extract, 5-90 parts of tremella extract mixing；
Alternatively, by weight, by 5-90 parts of licorice powders, 10-50 parts of tremella powder, the 10-90 parts of resulting water shields of step (1)
Powder is added to the water as raw material, and the volume additional amount of water is at least 40 times of volume shared by raw material dry weight, keeps the temperature 1- at 60 DEG C
It 2 hours, is extracted 0.5-2 hours at a temperature of at least 60 DEG C；
(3) by step (2) gains under at least 6000 rpm, it is centrifuged 10-30 min, takes supernatant；
(4) step (3) gained sediment is extracted and is separated with step (2) and step (3) the method, take supernatant
Liquid, and merge with supernatant obtained by step (3)；
(5) step (4) gains are crossed into polyamide column, spends centrifugation water elution；
(6) by step (5) efflux filter, be concentrated into 20 times of volume shared by raw material dry weight to get.
The country is concentrated mainly on to Polysaccharide of Brasenia Schreberi semifinished product extraction process the research of water shield, and extracting method is more multiple
It is miscellaneous, and it is easy to extract the impurity such as pigment tannin, and polysaccharide viscosity is big, it is difficult to it purifies, to influence product quality.Water shield attachment
Colloid picking a period of time after be easy to fall off, it is easily rotten due to more containing moisture, traditional furnace drying method time-consuming and
Cause the discoloration of water shield leaf serious, freeze drying efficiency is low and at high cost.Fresh tender leaf colloid is best in quality, and with the production of summer water shield
Measure highest, how largely good water shield saves and do not destroy its colloid and is a problem to be solved by summer.
Recovery rate to Polysaccharide of Brasenia Schreberi of the invention at least can achieve 0.6 mg/ml, and not use organic solvent,
Without containing sundries such as pigment tannings, not only production cost is low, it is often more important that particularly suitable as preparing preparation for external application to skin
Raw material.
Preferably, in step (1), the water shield powder is that volume is added and is no more than water shield leaf institute after cleaning fresh water shield
The pure water for accounting for volume handles 30 min using refiner under 4000rpm, be spray-dried at 60 DEG C obtained by；Step
(5) in, the packing volume of polyamide column is 3-4 times of extracting solution, and flow velocity is 1.0 ml/min, the deionization washing of 1 times of column volume
Column collects efflux；Filter method is successively with 200 mesh gauzes, filter paper, 5 μm of membrane filtrations in step (6)；Method for concentration packet
Include rotary evaporation, ultrafiltration, dialysis.
In step 1, fresh water shield contains large quantity of moisture (about 97%), and external colloid is abundant (965g volume is 1000ml), adds
Enter a small amount of pure water and is unfavorable for homogenate and spray drying when additional amount is greater than monoploid product.Homogenate 4000rpm, 30min can be incited somebody to action
Blade is broken, and degree of grinding is moderate.60 DEG C of dryings will not destroy its ingredient, and drying efficiency liquid is ideal.
In step 2, water shield and tremella ratio are slightly more, and the control of Radix Glycyrrhizae ratio hereinafter, Radix Glycyrrhizae is excessive, influences extracting solution 30%
Color.Since water shield and tremella water absorption are big, polysaccharide water absorption and swelling forms viscous solution, therefore 40 times of volumes or more are extracted, low
It is sticky in the volume extracting solution, it is unfavorable for effective component dissolution and subsequent processing.It is impregnated before extracting with warm water, so that dry powder absorbs water
Swelling, especially water shield powder and tremella powder are conducive to the dissolution of polysaccharide, then homogenate extracts.
Parameter of noncentricity extracts fluid viscosity height, and 6000rpm could separate blade with clear liquid, needs 6000rpm or more,
10min can reach desired separated effect.
The adsorbable wherein tannin of polyamide, and adsorption effect is better than macroreticular resin and zwitterion resin, passes through adsorbance
It investigates, amount of filler: when loading volume is 3:1, reaching preferable adsorption effect, 1.0 ml/min of flow velocity can be abundant under this flow velocity
Absorption is higher than this flow velocity, and detanning matter is insufficient, tannin test positive, and sample liquid viscosity is high, is extremely difficult to high flow rate.
Efflux is concentrated into 20 times of volumes, viscosity and effective component moderate concentration.
Preferably, in step (2), by weight, licorice powder is 10-30 parts, and tremella powder is 20-60 parts, and water shield powder is
30-60 parts.
It is another object of the present invention to provide preparing the application on preparation for external application to skin using stoste of the present invention.Skin
Skin external preparation includes facial mask, Essence, lotion, emulsifiable paste, gel preparation.
It should be understood that due to the present invention have the function of it is very outstanding, the present invention suitable for all need moisturizing or
Anti-oxidation function or the preparation for external application to skin for removing free radical, not cited by the limitation present invention.
The facial mask includes 20-50% stoste, 0.05-5% hyaluronic acid, 0.5-5% jojoba oil by mass percentage；
The lotion includes 20-50% stoste, 0.05-5% hyaluronic acid, 0.5-5% jojoba oil, 1-10% lecithin by mass percentage
Rouge；The Essence includes 20-50% stoste, 0.05-5% hyaluronic acid by mass percentage.
Beneficial effects of the present invention:
1, raw material is easy to get, is cheap；
2, with very outstanding performance of keeping humidity, antioxygenic property and removing free radical performance；
3, preparation process is simple, is not necessarily to expensive device, low production cost；
4, organic solvent, pigment tanning are not contained, does not constitute any injury to human health.
Detailed description of the invention
Fig. 1 is the infrared spectrogram of water shield extracting solution；
Fig. 2 is performance of keeping humidity effect picture of the invention；
Fig. 3 is antioxygenic property effect picture of the invention.
Specific embodiment
Specific embodiment by the following examples is described in further detail above content of the invention, but not
The range that this should be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.
Embodiment 1
1. the preparation of water shield powder: choosing fresh water shield 1000g, clear water is cleaned, volume 1000ml, equal pulp grinder
4000rpm, 30min, 60 DEG C of spray drying, obtain water shield powder, spare.
2 stoste extracting methods:
Pure water 1200ml is added in a water shield powder 10g, licorice powder 10g, tremella powder 10g, and 60 DEG C of heat preservations are swollen 1 hour, homogenate
It extracts 2 hours.
B is centrifuged (6000rpm, 10min), collects supernatant, and precipitating is extracted again, merges supernatant.
C supernatant crosses polyamide column, deionized water elution.
The filtering of d efflux is filtered using 200 mesh filtered through gauze, then filter paper, 5 micron membrane filters, is concentrated by ultrafiltration to 20 times and does
Sample bulking value 600ml to get.Stoste obtained is clear viscous solution, slightly opalescence, and viscosity is high.
Embodiment 2
1. the preparation of water shield powder: choosing fresh water shield 1000g, clear water is cleaned, volume 1000ml, equal pulp grinder
4000rpm, 30min, 60 DEG C of spray drying, obtain water shield powder, spare.
2 stoste extracting methods:
Pure water 1200ml is added in a water shield powder 15g, licorice powder 5g, tremella powder 10g, and 60 DEG C of heat preservations are swollen 1 hour, and homogenate mentions
It takes 2 hours.
B is centrifuged (6000rpm, 10min), collects supernatant, and precipitating is extracted again, merges supernatant.
C supernatant crosses polyamide column, deionized water elution.
The filtering of d efflux is filtered using 200 mesh filtered through gauze, then filter paper, 5 micron membrane filters, is concentrated by ultrafiltration to 20 times and does
Sample bulking value 600ml to get.Stoste obtained is clear viscous solution, slightly opalescence, and viscosity is high.
Embodiment 3
1. the preparation of water shield powder: choosing fresh water shield 1000g, clear water is cleaned, volume 1000ml, equal pulp grinder
4000rpm, 30min, 60 DEG C of spray drying, obtain water shield powder, spare.
2 stoste extracting methods:
Pure water 1600ml is added in a water shield powder 20g, licorice powder 10g, tremella powder 10g, and 60 DEG C of heat preservations are swollen 1 hour, homogenate
It extracts 2 hours.
B is centrifuged (6000rpm, 10min), collects supernatant, and precipitating is extracted again, merges supernatant.
C supernatant crosses polyamide column, deionized water elution.
The filtering of d efflux is filtered using 200 mesh filtered through gauze, then filter paper, 5 micron membrane filters, is concentrated by ultrafiltration to 20 times and does
Sample bulking value 800ml to get.Stoste obtained is clear viscous solution, slightly opalescence, and viscosity is high.
Embodiment 5
In addition to water shield powder used is 90g, licorice powder is 50g and tremella powder is 90g, remaining is consistent with embodiment.
Embodiment 6
In addition to water shield powder used is 10g, licorice powder is 10g and tremella powder is 5 g, remaining is consistent with embodiment.
Embodiment 7
Moisturizing essence production.
Formula such as table 1.
Table 1
Embodiment 8
Moisturizing emulsion production.
Formula such as table 2.
Table 2
Embodiment 9
Gel-type making face mask.
Formula such as table 3.
Table 3
Embodiment 10
Water shield powder addition pure water, 20 times of dry weight volumes, heating extraction 2 hours；It is centrifuged (6000rpm or more, 10min),
Supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values to get water shield
Gel extraction liquid.
Licorice powder addition pure water, 20 times of dry weight volumes, heating extraction 2 hours；It is centrifuged (6000rpm or more, 10min),
Supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values to get water shield
Gel extraction liquid.
Tremella powder addition pure water, 20 times of dry weight volumes, heating extraction 2 hours；It is centrifuged (6000rpm or more, 10min),
Supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values to get water shield
Gel extraction liquid.
By 90 parts of water shield extracting solution, 50 parts of licorice extract, 90 parts of tremella extract mixing.
Embodiment 11
Water shield powder addition pure water, 20 times of dry weight volumes, heating extraction 0.5 hour；Centrifugation (6000rpm or more,
10min), supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values,
Up to water shield gel extraction liquid.
Licorice powder addition pure water, 20 times of dry weight volumes, heating extraction 0.5 hour；Centrifugation (6000rpm or more,
10min), supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values,
Up to water shield gel extraction liquid.
Tremella powder addition pure water, 20 times of dry weight volumes, heating extraction 0.5 hour；Centrifugation (6000rpm or more,
10min), supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values,
Up to water shield gel extraction liquid.
By 10 parts of water shield extracting solution, 10 parts of licorice extract, 5 parts of tremella extract mixing.
Embodiment 12
Water shield powder addition pure water, 20 times of dry weight volumes, heating extraction 1 hour；It is centrifuged (6000rpm or more, 10min),
Supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values to get water shield
Gel extraction liquid.
Licorice powder addition pure water, 20 times of dry weight volumes, heating extraction 1 hour；It is centrifuged (6000rpm or more, 10min),
Supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values to get water shield
Gel extraction liquid.
Tremella powder addition pure water, 20 times of dry weight volumes, heating extraction 1 hour；It is centrifuged (6000rpm or more, 10min),
Supernatant is collected, precipitating is extracted again, merges supernatant；Supernatant liquid filtering is concentrated into 20 times of dry sample bulking values to get water shield
Gel extraction liquid.
By 50 parts of water shield extracting solution, 40 parts of licorice extract, 20 parts of tremella extract mixing.
Experimental example 1
Water shield extracting solution constituent analysis (infrared analysis)
Water shield extracting solution obtained by embodiment 10-12 is further processed rear 500-4000cm-1 infrared scan analysis, as a result
Display main component is polysaccharide: 3432cm-1(O-H), 2919cm-1(C-H), 1627cm-1(-C=O) and, 1423cm-1 (- C-
), O 1045cm-1 (C-OH), as shown in Figure of description 1.
Experimental example 2
Humidity-preserving type measurement is carried out to stoste obtained by embodiment 1-3.Stoste moistening effect of the present invention is best.Hyaluronic acid 0.1%
Have a certain viscosity, thus retentiveness it is minimum compared with viscosity essence lotion it is good, however Lancome essence lotion moisturizing rate is 12% after 24 hours.
Moisturizing rate calculates, and under RH60% constant humidity, for the actual conditions of better simulated skin, selects appropriately sized
Glass plate stick 3M micro-porous permeable adhesive tape (1 × 2cm), amount of samples about 0.05g/cm2.Aqueous solution is coated in and posts 3M
It on adhesive tape, puts constant humidity drier into and weighs after a certain period of time, calculate moisturizing rate.
It calculates: moisturizing rate %=(1- (M1-M2)/m) × 100
Moisture weight after M2 mono- is placed
M1 mono- places preceding moisture weight
Mono- coating weight of m
Experimental example 3
Inoxidizability test is carried out to stoste obtained by embodiment 1-3.Pyrogallol autoxidation under alkaline condition, constantly
Release O2-, O2-Autoxidation can further be promoted again, after mouse thymus cells 30s, intermediary accumulated concentrations are in the time
Linear relationship, general linear time can maintain 4min, can acquire oxidation rate to characterize O2-Understand ability.Clearance rate=(in formula: A0 is the blank absorbency of non-dosing solution;Ai is the absorbance of solution after dosing;Aj is sample solution
The absorbance of itself, pyrogallol oxidation system color products have reached stabilization when 9min, thus radicals scavenging effect with
Light absorption value calculates when 9min.)
4, table anti-oxidant experimental group sample-addings table (n=3)
Table 4
Pyrogallol 0.5ml is added in reaction system, cuvette, while manual time-keeping are poured into shaking immediately.All-wave length
30s, 60s, 90s, the UV absorption figure of 120s, 180s are scanned, 320nm goes out absorbance and increases increase with time.Select 320nm
Absorbance is measured out.
1 measurement system autoxidation rate of group is 0.0036(y=0.0036x+0.2587；R2=0.9910)；2 measurement sample-adding of group
0.0013 (y=0.0013x+0.2318 of oxidation rate afterwards；R2=0.9832)；Group 3 is blank control；4 measurement sample itself of group is inhaled
Luminosity.Measurement result such as Fig. 3, after stoste (i.e. compound fluid) of the present invention is added, oxidation rate is decreased obviously, and has stronger removing
Free radical ability, free radical scavenging activity 49.01%.
Experimental example 4
The evaluation of extracting solution antioxidant activity obtained by Different Extraction Method
The antioxidant activity of alkali extracting solution is slightly below Aqueous extracts, and more impurity is contained mainly in alkali extract, affects it
Antioxidant activity, and color is brown, is not used to cosmetics；Ethanol extract equally has preferable antioxidant activity, still
Pigment etc. is more, and color is deeper, and acts on without moisture retention.The oxidation rate of Aqueous extracts is 0.0013(y=0.0013x+0.2183,
R2=0.9915), the oxidation rate of alkali extract is 0.0018(y=0.0018x+0.2703, R2=0.9816), alcohol extract oxidation rate
For 0.0011(y=0.0011x+0.2874, R2=0.9682).
Experimental example 5
Organic free radical scavenging capacity detection.DPPH existsOrganic solventIn be a kind of stable free radical, alcoholic solution is in
Purple, and low-temperature dark is needed to store has single electronics, thus can receive an electronics orHydrogen ion, have in the case where wavelength is 517nm
There is absorption maximum.HaveFree radical scavengerIn the presence of, the single electron of DPPH is captured and makes its lighter, in maximumLight absorption
Light absorption value decline at wavelength, and decline degree and be inLinear relationship,AbsorbanceHorizontal reduction shows antioxidative increase, from
And with the oxidation resistance of evaluation test sample.This oxidation resistance indicates that inhibiting rate is bigger with inhibiting rate, and inoxidizability is got over
By force.
Experimental material: DPPH reagent, extracting solution sample.
Experimental method:
DPPH preparation of reagents: DPPH (0.2mM) 0.0079g, 50% ethyl alcohol SDS 1.5% dissolution are settled to 100ml, now match
It is current.Experimental group: DPPH 2ml, extracting solution 1ml, retarder thinner 2ml, blank group: DPPH 2ml, water 1ml, retarder thinner 2ml,
Sample sets: extracting solution 1ml, retarder thinner 4ml.
Radicals scavenging effect after stoste of the present invention is added in experimental group measurement, and blank group measures DPPH itself under equal conditions
Absorbance, sample sets measure sample itself absorbance.The absorbance A that measurement solution changes over time, every 60s value 1 time, wait inhale
When luminosity is basically unchanged, extracting solution is complete with radical reaction, absorbance when record is stablized.After experiment shows reaction 1 hour, base
This stabilization.
Clearance rate=(in formula: A0 is the blank absorbency of non-dosing DPPH solution;Ai is DPPH after dosing
The absorbance of solution;Aj is the absorbance of sample solution itself.)
5 organic free radical clearance rate (n=3) of table
Water shield extracting solution: 20g, 40 times of volumes are extracted, and are concentrated into 20 times after purification, measurement.
Licorice extract: 20g, same method handle to obtain.
Compound extract: water shield 10g, Radix Glycyrrhizae 10g, same method handle to obtain.
Claims (7)
1. a kind of preparation for external application to skin stoste, which is characterized in that the stoste is by weight, comprising:
10-90 parts of water shield extracting solution
10-50 parts of licorice extract
5-90 parts of tremella extract；
In the water shield extracting solution, polyoses content is at least 0.6 mg/mL, and water shield total polyphenols are at least 1.2 × 10-4Mg/mL,
Without pigment, tannin and organic solvent；Glycyrrhizic acid is at least 2.08 × 10 in the licorice extract-3mg/mL；The tremella mentions
Tremella polysaccharides in liquid are taken to be at least 2.5 × 10-3mg/mL。
2. stoste according to claim 1, which is characterized in that the stoste is by weight, comprising:
30-60 parts of water shield extracting solution
10-30 parts of licorice extract
20-60 parts of tremella extract；
3. the preparation method of stoste as described in claim 1, which is characterized in that described method includes following steps:
(1) prepared by water shield powder: fresh water shield being homogenized and is spray-dried again, water shield powder is obtained, is stored；
(2) with water to licorice powder, tremella powder, water shield powder extracts respectively obtained by step (1), obtain respectively licorice extract,
Tremella extract, water shield extracting solution；It controls in water shield extracting solution, polyoses content is at least 0.6 mg/ml, and water shield total polyphenols are at least
It is 1.2 × 10-4Mg/mL is free of pigment, tannin and organic solvent；In control licorice extract glycyrrhizic acid be at least 2.08 ×
10-3mg/mL；Tremella polysaccharides are at least 2.5 × 10 in control tremella extract-3mg/mL；By weight, by water shield extracting solution
10-90 parts, 10-50 parts of licorice extract, 5-90 parts of tremella extract mixing；
Alternatively, by weight, 5-90 parts of licorice powders, 10-50 parts of tremella powder, the resulting water shield powder of 10-90 parts of steps (1) are made
It is added to the water for raw material, the volume additional amount of water is at least 40 times of volume shared by raw material dry weight, and it is small that 1-2 is kept the temperature at 60 DEG C
When, it is extracted 0.5-2 hours at a temperature of at least 60 DEG C；
(3) by step (2) gains under at least 6000 rpm, it is centrifuged 10-30 min, takes supernatant；
(4) step (3) gained sediment is extracted and is separated with step (2) and step (3) the method, take supernatant,
And merge with supernatant obtained by step (3)；
(5) step (4) gains are crossed into polyamide column, spends centrifugation water elution；
(6) by step (5) efflux filter, be concentrated into 20 times of volume shared by raw material dry weight to get.
4. according to the method described in claim 3, it is characterized in that, the water shield powder is to clean fresh water shield in step (1)
Afterwards, the pure water that volume is no more than volume shared by water shield leaf is added, 30 min are handled under 4000rpm using refiner, then at 60
Obtained by being spray-dried at DEG C；In step (5), the packing volume of polyamide column is 3-4 times of extracting solution, and flow velocity is 1.0 mL/
The deionization of min, 1 times of column volume wash column, collect efflux；Filter method is successively with 200 mesh gauzes, filter in step (6)
Paper, 5 μm of membrane filtrations；Method for concentration includes rotary evaporation, ultrafiltration, dialysis.
5. according to the method described in claim 3, it is characterized in that, in step (2), by weight, licorice powder 10-30
Part, tremella powder is 20-50 parts, and water shield powder is 30-60 parts.
6. stoste as claimed in claim 1 or 2 is preparing the application on preparation for external application to skin, which is characterized in that the external preparation for skin
Preparation includes facial mask, Essence, lotion, emulsifiable paste, gel preparation.
7. application according to claim 6, which is characterized in that the facial mask includes 20-50% original by mass percentage
Liquid, 0.05-5% hyaluronic acid, 0.5-5% jojoba oil；The lotion includes 20-50% stoste, 0.05- by mass percentage
5% hyaluronic acid, 0.5-5% jojoba oil, 1-10% lecithin；The Essence include by mass percentage 20-50% stoste,
0.05-5% hyaluronic acid.
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| CN102078271A (en) * | 2010-11-26 | 2011-06-01 | 新时代健康产业（集团）有限公司 | Skin care composition with moisturizing efficacy and preparation and preparation method thereof |
| CN102579290A (en) * | 2011-01-07 | 2012-07-18 | 李庆华 | Extraction method for gelatin in water shield |
| CN104161715A (en) * | 2014-06-26 | 2014-11-26 | 吴雪琴 | Preparing method and product of whitening skincare lotion |
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| CN102078271A (en) * | 2010-11-26 | 2011-06-01 | 新时代健康产业（集团）有限公司 | Skin care composition with moisturizing efficacy and preparation and preparation method thereof |
| CN102579290A (en) * | 2011-01-07 | 2012-07-18 | 李庆华 | Extraction method for gelatin in water shield |
| CN104161715A (en) * | 2014-06-26 | 2014-11-26 | 吴雪琴 | Preparing method and product of whitening skincare lotion |
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