CN102309472B - Compound essential oil with anti-free radical function - Google Patents
Compound essential oil with anti-free radical function Download PDFInfo
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Abstract
The invention relates to compound essential oil with an anti-free radical function. The compound essential oil comprises the following components of dl-citronellol, eugenol, citral, eugenol methyl ether and thymol. A measurement method which is widely used for analyzing the anti-oxidation capacity of substances comprise the following step of: detecting the capacity of eliminating 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, the capacity of eliminating 2,2'-azino-bis(3-ethylbenzthiazoline-6-suphonic acid)<+>(ABTS<+>) free radicals, the capacity of chelating metal ions and reduction capacity, so the condition that the compound essential oil really has high anti-free radical capacity and anti-oxidation capacity is proved.
Description
Technical field
The present invention relates to a kind of compound essential Oil, the compound essential Oil that refers to a kind of tool anti-free radical function is particularly arranged.
Background technology
American chemical expert Dr.Harman confirms in the DrugsAging periodical that in the time of 1993 too much free radical is the major reason that causes biological aging.Yet, no matter be the course of reaction of organism autoxidation metabolism, or the ionizing radiation in the external environment, and such as the environmental contaminants of air pollution and chemical substance etc., all can make and constantly produce free radical (free radical) and derivant thereof in the organism.With regard to human, be coated on the easiest impact that is subject to external environment of the outer field skin of human body, for example the ultraviolet in the environment can make fibroblast and grain line body in the dermis of skin be upset, and then discharges superoxide anion (superoxide anion, O
2 -), excessive superoxide anion then can convert the stronger free radical of other destructiveness to.Although have in the human body be used to the Antioxidative Defense System of keeping poised state between the oxidative and anti-oxidative, can slow down active oxygen (reactive oxygen species, ROS) and the generation of free radical, yet, if long-term excessive tanning by the sun under sunlight, then a large amount of free radicals that produce will cause the anti-oxidative defense ability of skin to reduce in the human body, and then cause the acute or chronic injury of skin, such as photoaging (photoaging), creasy surface, the imbalance of skin immunization power and skin carcinoma etc.
Therefore, having occurred the many products that can remove free radical and have anti-oxidation function of bragging about on the market, mainly is by it being applied in skin surface, reaching the effect that increases the human body skin oxidation resistance.Yet the molecule of contained antioxidant content is all large or be water solublity in the better product of most of oxidation resistances, causes human body skin to be difficult to absorption, so the actual oxidation resistance that can reach is limited.
On the other hand, the various compound essential Oil that emerge for satisfying consumer's uphold nature now, also since some special essential oil contain can the antioxidative composition, and its molecule makes characteristics that human body skin absorbs than Xiao Yi and popular, by incense, smear, massage or the mode such as immersion, the antioxidant content in the quintessence oil is absorbed by Skin Cell and respiratory system, infiltrate skin histology through the conduction of pore again, then arrive systemic cell, finally reach the antioxidative effect.Yet bragging about of selling on the market at present can the antioxidative compound essential Oil, does not have objective experimental data and confirms that these compound essential Oil have the free radical of removing and antioxidative ability really.
Summary of the invention
For the problems referred to above, main purpose of the present invention is to provide a kind of compound essential Oil of tool anti-free radical function, and it has than commercially available compound essential Oil through experiment confirm really is strong antiradical activities and oxidation resistance.
For achieving the above object, the compound essential Oil of a kind of tool anti-free radical function provided by the present invention, it consists predominantly of citronellol (dl-Citronellol), eugenol (eugenol), citral (cis/trans-Citral), Eugenol methyl ether (Eugenol methyl ether) and thymol (Thymol).
Wherein include the citronellol of 0.5～8wt%, the eugenol of 4.5～24.6wt%, the citral of 3.75～20wt%, the Eugenol methyl ether of 0.15～12.5wt% and the thymol of 4.5～25.2wt%.
This citronellol is for taking from the Flos Pelargonii quintessence oil.
This eugenol is for taking from cinnamon essential oil.
This citral is for taking from the lemon grass (Cymbopogon citratus) quintessence oil.
This Eugenol methyl ether is for taking from western India laurin oil.
This thymol is for taking from wild horse Yu Lan quintessence oil.
Adopt technique scheme, because the contained chemical constituent of compound essential Oil of the present invention, be and have splendid scavenging ability of DPPH free radical in the plants essential oil, remove ABTS
+The chemical constituent of free radical ability, chelated metal ions ability and reducing power, therefore compound essential Oil of the present invention can show excellent antiradical activities and oxidation resistance.
The specific embodiment
The present invention is described in detail now to lift following examples.
The compound essential Oil of a kind of tool anti-free radical function provided by the present invention, it consists predominantly of citronellol, eugenol, citral, Eugenol methyl ether and thymol composition.
The compound essential Oil that a preferred embodiment of the present invention provides, it includes the citronellol of 0.5～8wt%, the eugenol of 4.5～24.6wt%, the citral of 3.75～20wt%, the Eugenol methyl ether of 0.15～12.5wt% and the thymol of 4.5～25.2wt%.
Wherein, although phenols has fabulous removal free radical ability, excessive phenols can produce pungent taste, and therefore the content of above-mentioned each composition is the optimal proportion of testing repeatedly gained, can when possessing removal free radical ability, can also take into account the abnormal smells from the patient of compound essential Oil.
The applicant at first must illustrate at this, since be used for extracting quintessence oil plant the place of production and planting type, the opportunity of plucking a plant, extraction quintessence oil method and extract the factors such as position, the difference of chemical composition content in the quintessence oil is caused in the capital, therefore the quintessence oil of hereinafter mentioning, the content of its chemical constituent all can change in a scope.
This citronellol is for taking from the Flos Pelargonii quintessence oil, and account for the Flos Pelargonii quintessence oil 5 to 40wt%.This eugenol be take from cinnamon essential oil and account for cinnamon essential oil 30 to 82wt%.This citral be take from the lemon grass (Cymbopogon citratus) quintessence oil and account for the lemon grass (Cymbopogon citratus) quintessence oil 25 to 80wt%.This Eugenol methyl ether be take from western India laurin oil and account for western India laurin oil 0.1 to 5wt%.This thymol be take from wild horse Yu Lan quintessence oil and account for wild horse Yu Lan quintessence oil 30 to 84wt%.
The western India laurin oil of the Eugenol methyl ether composition that the following embodiment of the present invention is Flos Pelargonii quintessence oil, the cinnamon essential oil that contains the eugenol composition of 82wt%, the lemon grass (Cymbopogon citratus) quintessence oil that contains the citral composition of 80wt% that will contain the citronellol composition of 40wt%, contain 5wt% and the wild horse Yu Lan quintessence oil that contains the thymol composition of 84wt% evenly mix to make compound essential Oil in different ratios.
Wherein, the employed method of aforesaid mixing can be used and generally know the whole bag of tricks that can reach even mixing purpose, and there is no particular restriction.
The below enumerates following example and further illustrates the present invention; yet these embodiment only are used for more understanding the present invention; rather than for limiting scope of patent protection of the present invention; have in the technical field under all and usually know the knowledgeable, do not violating the various variations of doing under the creation spirit of the present invention and modifying all genus categories of the present invention.
Embodiment 1
The wild horse Yu Lan quintessence oil of the western India laurin oil of the lemon grass (Cymbopogon citratus) quintessence oil of the cinnamon essential oil of the Flos Pelargonii quintessence oil of 10g, 30g, 15g, 15g and 30g is mixed into the compound essential Oil of total amount 100g, so, contains the citronellol of 4wt% in this compound essential Oil; The eugenol of 24.6wt%; The citral of 12wt%; The Eugenol methyl ether of 0.75wt%; And the thymol of 25.2wt%.
Embodiment 2
The wild horse Yu Lan quintessence oil of the western India laurin oil of the lemon grass (Cymbopogon citratus) quintessence oil of the cinnamon essential oil of the Flos Pelargonii quintessence oil of 10g, 25g, 20g, 20g and 25g is mixed into the compound essential Oil of total amount 100g, so, contains the citronellol of 4wt% in this compound essential Oil; The eugenol of 20.5wt%; The citral of 16wt%; The Eugenol methyl ether of 1wt%; And the thymol of 21wt%.
Embodiment 3
The wild horse Yu Lan quintessence oil of the western India laurin oil of the lemon grass (Cymbopogon citratus) quintessence oil of the cinnamon essential oil of the Flos Pelargonii quintessence oil of 20g, 15g, 25g, 25g and 15g is mixed into the compound essential Oil of total amount 100g, so, contains the citronellol of 8wt% in this compound essential Oil; The eugenol of 12.3wt%; The citral of 20wt%; The Eugenol methyl ether of 1.25wt%; And the thymol of 12.6wt%.
Comparative example 1
The easypro work that The Body Shop (The Body Shop) is sold improves compound essential Oil, its include organic Fructus Citri Sarcodactylis, Fructus Citri sinensis, grapefruit, rosewood, Cymbopogon martini, Herba Rosmarini Officinalis, Herba Menthae, Rhizoma Zingiberis Recens, Fructus Citri Limoniae, Cedrus deoclar (Roxb.) G. Don and Herba Pogostemonis composition.
Comparative example 2
The scent virtue that DHC sells is treated essential oils (the wooden perfume (or spice) of Citrus), and it includes Citrus, Fructus Citri Sarcodactylis, orange, orange blossom, Lignum pterocarpi indici, Cedrus deoclar (Roxb.) G. Don and Lignum Santali Albi composition.
Comparative example 3
Le Bon moment sells improves the excitation compound essential Oil, and it includes gin, permanent flower, rose wood, Herba Rosmarini Officinalis and Herba Menthae composition.
Comparative example 4
The lucky star shines bright compound essential Oil that Luo Ruina sells, it includes Damascus Flos Rosae Rugosae, Myrrha, Herba Pogostemonis, Vetiveria zizanoides, Rhizoma Zingiberis Recens and rosewood composition.
Comparative example 5
The orange liquor-saturated romantic compound essential Oil that Luo Ruina sells, it includes Petitgrain, Flos Pelargonii, Salvia sclarea, cypress, rosewood and grapefruit composition.
Comparative example 6
The glamour that Luo Ruina sells is put the indigo compound essential Oil, its include pelargonium roseum, Herba Rosmarini Officinalis, Herba Ocimi (Herba Ocimi Pilosi), Lignum cinnamomi camphorae, highland Garden lavender and Flos Pelargonii composition.
Comparative example 7
Luo Ruina sells that The Purple Air comes from the east compound essential Oil, it includes highland Garden lavender, rosewood, Olibanum, Myrrha and Herba Ocimi (Herba Ocimi Pilosi) composition.
Comparative example 8
The spotless and flawless compound essential Oil that Luo Ruina sells, it includes highland Garden lavender, grapefruit, Vetiveria zizanoides, Lignum cinnamomi camphorae and Fructus Citri Sarcodactylis composition.
Adopt method as described below to detect the compound essential Oil of above embodiment and each comparative example, to assess its antiradical activities and oxidation resistance.Assessment result is as shown in table 1.
The scavenging ability of DPPH free radical test
First embodiment 1 to 3 is formulated as respectively the solution that concentration is 10mg/mL with comparative example 1 to 8.
Again 1,1-diphenyl-2-picryl hydrazine (TCI is for selling for 1,1-Diphenyl-2-picrylhydrazyl, DPPH) is dissolved in the ethanol, to be formulated as the DPPH alcoholic solution of concentration 1.52 * 10-4M.
Then, respectively get the solution 500 μ L of previous embodiment 1 to 3 and comparative example 1 to 8, and respectively with this DPPH alcoholic solution concussion mix homogeneously of 2500 μ L after, at room temperature lucifuge left standstill 30 minutes, as experimental group.
In addition, matched group is with the solution of the embodiment 1 to 3 in butylated hydroxytoluene (BHT) the replacement previous experiments group of 500 μ L with comparative example 1 to 8.
Then utilize ultraviolet light/visible spectrometry (UV-2001Spectrophotometer, HITACHI company is for selling) to measure this experimental group and the light absorption value of this matched group when wavelength 517nm.
The power of scavenging ability of DPPH free radical is to assess with following equation 1.The ability that the DPPH free radical is removed in the higher expression of numerical value is stronger.
Equation 1
Remove ABTS
+The free radical aptitude tests
First embodiment 1 to 3 and comparative example 1 to 8 are formulated as respectively the solution of concentration 1mg/mL.
Again with the hydrogen peroxide (H of the concentration 500 μ M of the deionized water of 250 μ L, 250 μ L
2O
2) ABTS (2 of concentration 1000 μ M of solution, 250 μ L, the peroxidase (Peroxidase) of the concentration 8.8U/mL of 2 '-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) solution and 250 μ L, (VortexGenie 2 with multipurpose test tube agitator, Scientific Industries company is for selling) mix homogeneously, when after 10 minutes, producing stable aeruginous ABTS+ radical cation, add respectively immediately the previous embodiment 1 to 3 of 250 μ L and the solution of comparative example 1 to 8.After using afterwards multipurpose test tube agitator mix homogeneously, standing and reacting 10 minutes is as experimental group.
In addition, matched group is take butylated hydroxyanisol (butylated hydroxyl anisole, BHA) as standard substance, with the solution of the embodiment 1 to 3 in the deionized water replacement previous experiments group of 250 μ L with comparative example 1 to 8.
Then utilize immune enzymatic analysis instrument (model Sunrise-Basic-Tecan, Austrian Tecan company is for selling for Enzyme-linked immunoassay, ELISA) to measure this experimental group and the light absorption value of this matched group when wavelength 410nm.
Remove ABTS
+The power of free radical ability is to assess with following equation 2.ABTS is removed in the higher expression of numerical value
+The ability of free radical is stronger.
Equation 2
The chelated metal ions aptitude tests
First embodiment 1 to 3 and comparative example 1 to 8 are formulated as respectively the solution of concentration 1mg/mL.
Iron chloride (Iron (II) chloride) solution with the concentration 2mM of 750 μ L mixed for 30 seconds with the solution concussion of comparative example 1 to 8 with the previous embodiment 1 to 3 of 500 μ L respectively with the methanol of 250 μ L again.Then luxuriant and rich with fragrance Lip river piperazine (Ferrozine) solution that adds the concentration 5mM of 1500 μ L, and lucifuge left standstill 30 minutes under room temperature.After complete reaction, with rotating speed 3000rpm centrifugal 5 minutes, get afterwards supernatant liquid, as experimental group.
In addition, matched group is with the solution of the embodiment 1 to 3 in the ethylenediaminetetraacetic acid of 500 μ L (ethylenediaminetetraacetic acid, EDTA) the replacement previous experiments group with comparative example 1 to 8, and does not carry out centrifugation step.
Then utilize ultraviolet light/visible spectrometry (UV-2001Spectrophotometer, HITACHI company is for selling) to measure this experimental group and the light absorption value of this matched group when wavelength 562nm.
The power of chelated metal ions ability is to assess with following equation 3, and the ability of the higher expression chelated metal ions of numerical value is stronger.
Equation 3
The reducing power test
First embodiment 1 to 3 is formulated as respectively the solution that concentration is 10mg/mL with comparative example 1 to 8.
Sodium dihydrogen phosphate (sodium dihydrogenphosphate) solution of concentration 0.2M being become concentration 2mM and pH value with sodium hydrogen phosphate (disodium hydrogenphosphate) solution preparation of concentration 0.2M is 6.6 phosphate buffered solution (phosphate buffer solution) again.
Then, respectively get the solution 500 μ L of previous embodiment 1 to 3 and comparative example 1 to 8, and respectively with the potassium ferricyanide solution mix homogeneously of the concentration 1% of this phosphate buffered solution of 250 μ L and 250 μ L.Then under temperature 50 C, carry out water-bath 20 minutes, cooled off rapidly subsequently 5 minutes.The trichloroacetic acid solution mix homogeneously with it that adds afterwards the concentration 10% of 250 μ L.The supernatant liquid of 100 μ L is taken out, and add the ferric chloride solution mix homogeneously with it of the concentration 0.1% of the deionized water of 500 μ L and 750 μ L.At room temperature lucifuge leaves standstill after 30 minutes and namely gets experimental group.
In addition, matched group is take butylated hydroxyanisol as standard substance, replaces the previous experiments group with the ethanol of the concentration 99.8% of 500 μ L.
In the hole of the ferment immunoassay dish that then this experimental group and this matched group of 200 μ L is added respectively 96 holes, utilize immune enzymatic analysis instrument to measure this experimental group and the light absorption value of this matched group when wavelength 700nm.
The power of reducing power is to assess with following equation 4, and the higher expression reducing power of numerical value is stronger.
Equation 4
[table 1]
| Classification | DPPH free radical scavenging activity (%) | ABTS +Free radical scavenging activity (%) | Chelation percent (%) | Reducing power (%) |
| Embodiment 1 | 94.52±0.23 | 88.47±0.33 | 62.32±0.77 | 86.88±1.31 |
| Embodiment 2 | 88.02±0.55 | 83.97±1.21 | 58.08±1.81 | 80.64±0.98 |
| Embodiment 3 | 82.67±0.94 | 78.31±1.01 | 56.22±0.63 | 77.62±1.75 |
| Comparative example 1 | 42.12±1.65 | 37.33±1.82 | 21.39±2.06 | 33.98±1.87 |
| Comparative example 2 | 28.24±0.98 | 27.88±0.45 | 18.65±0.77 | 25.85±1.74 |
| Comparative example 3 | 22.11±1.82 | 19.38±2.27 | 11.34±0.69 | 16.15±0.71 |
| Comparative example 4 | 38.32±0.83 | 36.18±1.28 | 28.7±0.22 | 18.28±0.06 |
| Comparative example 5 | 44.12±0.69 | 40.37±0.24 | 27.68±0.69 | 14.17±0.13 |
| Comparative example 6 | 48.35±0.63 | 44.97±0.68 | 55.31±0.52 | 20.21±0.92 |
| Comparative example 7 | 33.62±0.12 | 30.92±0.77 | 51.32±1.37 | 13.80±1.45 |
| Comparative example 8 | 28.12±0.31 | 26.41±0.32 | 19.55±0.61 | 16.61±0.44 |
By the result of table 1 obviously as seen, the embodiment of the invention 1 to 3 scavenging ability of DPPH free radical, remove ABTS
+Free radical ability, chelated metal ions ability and reducing power are all far above comparative example 1 to 8.
In addition, carry out human experimentation for the compound essential Oil of embodiment 3 in the incense mode, the result shows that compound essential Oil of the present invention has the effect of removing free radical in the blood of human body.Experiment is analyzed for blood and the blood of incense after 10 minutes that 15 adults extract before the incenses, at first, 0.2ml blood is evenly mixed with 0.1ml 0.9% normal saline solution, and be warming up to 37 ℃ with simulation body temperature, 1ml reagent reinjects, this reagent is 2.5mglucigenin to be dissolved in the allotment of 50ml 0.9% normal saline solution form, utilize (the TohokuElectronic Co. of northeast Electronics Co., Ltd, Ltd) the determining free radicals instrument of manufacturing (Chemiluminescence Spectrometer) is measured free radical quantity, and measurement result is as shown in table 2:
[table 2]
| Numbering | Free radical quantity before the incense | Free radical quantity behind the incense | Free radical scavenging activity |
| 1 | 12136 | 5554 | 54.24％ |
| 2 | 15038 | 3020 | 79.92％ |
| 3 | 6283 | 5247 | 16.49％ |
| 4 | 20511 | 3840 | 81.28％ |
| 5 | 90235 | 31152 | 65.48％ |
| 6 | 98326 | 2144 | 97.82％ |
| 7 | 33897 | 14113 | 58.37％ |
| 8 | 59844 | 5877 | 90.18％ |
| 9 | 10673 | 3725 | 65.10％ |
| 10 | 26632 | 8820 | 66.88％ |
| 11 | 15351 | 7123 | 53.60％ |
| 12 | 47392 | 15997 | 66.25％ |
| 13 | 27340 | 2687 | 90.17％ |
| 14 | 39810 | 2357 | 94.08％ |
| 15 | 8991 | 4689 | 47.85％ |
| On average | 34164 | 7756 | 69％ |
By as seen from Table 2, by the incense mode, the compound essential Oil of embodiment 3 can effectively be removed the free radical in the blood of human body, average free radical scavenging activity even near 7 one-tenth, and effect is quite remarkable.
In gross, the compound essential Oil of the embodiment of the invention 1 to 3, its scavenging ability of DPPH free radical, removing ABTS
+The general performance of free radical ability, chelated metal ions ability and reducing power is all very excellent, and it is remarkable to confirm that through human trial it removes the free radical effect.Therefore, the compound essential Oil of tool anti-free radical function of the present invention can show excellent antiradical activities and oxidation resistance really.
Claims (1)
1. the compound essential Oil of a tool anti-free radical function is characterized in that including following composition:
The Flos Pelargonii quintessence oil, cinnamon essential oil, the lemon grass (Cymbopogon citratus) quintessence oil, west India's laurin oil and wild horse Yu Lan quintessence oil, wherein the Flos Pelargonii quintessence oil contains 5 to 40wt% citronellol, cinnamon essential oil contains 30 to 82wt% eugenol, the lemon grass (Cymbopogon citratus) quintessence oil contains 25 to 80wt% citral, west India laurin oil contains 0.1 to 5wt% Eugenol methyl ether, wild horse Yu Lan quintessence oil contains 30 to 84wt% thymol, makes this compound essential Oil contain 0.5 to 8wt% citronellol, 4.5 the eugenol to 24.6wt%, 3.75 the citral to 20wt%, 0.15 to the Eugenol methyl ether of 12.5wt% and 4.5 to 25.2wt% thymol.
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| CN102860941B (en) * | 2012-10-22 | 2013-06-05 | 上海韬鸿投资管理咨询有限公司 | Nursing compound essential oil for neutral skin |
| CN106492152A (en) * | 2016-11-02 | 2017-03-15 | 陈厚兵 | A kind of skin surface disinfecting anti inflammatory agent and preparation method thereof |
| CN110974730A (en) * | 2019-09-27 | 2020-04-10 | 中山大学新华学院 | Antioxidant compound cinnamon essential oil and application |
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| CN101057817A (en) * | 2007-05-16 | 2007-10-24 | 徐海波 | Pine nut skin-protection oil containing natural rose essential |
| US20090131539A1 (en) * | 2005-07-12 | 2009-05-21 | Frank Schneider | Mixture |
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| US20090131539A1 (en) * | 2005-07-12 | 2009-05-21 | Frank Schneider | Mixture |
| CN101057817A (en) * | 2007-05-16 | 2007-10-24 | 徐海波 | Pine nut skin-protection oil containing natural rose essential |
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