CN106305706A - Long-term seedling storing method capable of decreasing early bolting rate of angelica - Google Patents
Long-term seedling storing method capable of decreasing early bolting rate of angelica Download PDFInfo
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Abstract
The invention discloses a long-term seedling storing method capable of decreasing early bolting rate of angelica. According to the method, seedlings are stored at 5-15 DEG C; a refrigerator door is opened and closed at regular intervals so as to change air during the storage; seedlings cannot be stored by virtue of dry soil and need to be stored by virtue of 'damp soil'. Experiments prove that after being taken out after one year, the seedlings stored at -5 DEG C can still have good vitality, and the plating survival rate reaches 100%, so that the early bolting rate of the angelica seedlings stored within the temperature interval can be decreased, and the angelica seedlings can be stored for a long time.
Description
Technical field
The invention belongs to Chinese herbal medicine growing and cultivation technical field, particularly relate to a kind of length reducing Radix Angelicae Sinensis early a kind of sedge rate
Phase storage seedling-growing method.
Background technology
Radix Angelicae Sinensis is Umbelliferae angelica perennial herb medicinal plants, is one of famous important Chinese medicine material, tool
There is the highest medical value, have saying of " ten side's rules for doing division with a one-digit divisor on the abacus ".Theory of Chinese medical science is thought that working as is classified as enriching blood and is adjusted the product of blood,
For gynecological's common medicine, it is classified as grass portion fragrant grass class by Compendium of Material Medica, is called " woman's key medicine ".Radix Angelicae Sinensis contains
The excited two-way function with suppression uterine smooth muscle, can be used to regulating menstruation, it is also possible to be used for treating uterine prolapse
With the enuresis etc., the patient for menoxenia, blood deficiency and merging constipation is especially suitable.In addition Radix Angelicae Sinensis
Can be additionally used in cardiovascular and cerebrovascular disease, respiratory tract disease, osteoarthrosis, muscular tissue disease and senile disease
Sick etc..Radix Angelicae Sinensis is also widely used in the manufacture process of cosmetics, health product, beverage, wine etc..Radix Angelicae Sinensis
In Gansu, Sichuan, the ground such as Yunnan all have distribution and cultivation, but Gansu yield is most, account for total output 95% with
On.Gansu is with Minxian County as main product, and all there is cultivation on the ground such as periphery Zhang County, Weiyuan, wherein, with Minxian County Radix Angelicae Sinensis
Multimass is best, is described as " Min returns ".Minxian County, Gansu, Zhang County and periphery are semi-arid area, grassland, Gannan
District is bordered on south of Gansu Province mountain region, weather cold and damp, domestic height above sea level 2,040 3754 meters, average temperature of the whole year 5.7 DEG C,
120 days frost-free periods, normal year rainfall about 600mm, rainfall concentration, 6 JIUYUE account for the whole year
64.5%.Its special geographic climate, has bred the natural resources of uniqueness.Meanwhile, this condition also causes
Make the actual planting area of Radix Angelicae Sinensis the narrowest.This is also the major reason limiting Radix Angelicae Sinensis yield, and utilization has
The natural resources of limit improves Radix Angelicae Sinensis yield and is particularly important.Radix Angelicae Sinensis (Angelica sinensis [Oliv.] Diels)
It it is low temperature long day plant.Cultivation period is 3 years, First Year nursery, Second Year patent medicine, the 3rd year bolting knot
Seed.But the Second Year of Radix Angelicae Sinensis cultivation, was originally in the patent medicine Radix Angelicae Sinensis of vegetative growth phase, there are about 20%-30%'s
Plant bolting, is proceeded to reproductive growth by nourishing and growing, and this is the Early bolting phenomenon of Radix Angelicae Sinensis, is called for short early a kind of sedge.
Early a kind of sedge Radix Angelicae Sinensis, in root, secondary phloem is changed into 1:10 with the ratio of secondary xylem by normal 2:1, secondary
The parenchyma cell lignifying of xylem, the parenchyma cell of life and secretory canal reduce, and affect its secondary metabolite
Formed, therefore root bavin is big, lack oil gas, it is impossible to medicinal, make unit are patent medicine strain number reduce, yield
Loss is serious.About the physiological foundation of Radix Angelicae Sinensis early a kind of sedge, lack further investigation and report.Guess its Early bolting
Physiological foundation should with other with type plant Early bolting as, i.e. after low temperature vernalization, the long-day promotes it
Educate bolting to bloom.Vernalization is the plant reaction to winter low temperature, and after completing vernalization, plant overcomes and experiences light
The obstacle of cycle induced flowering.There is the biggest seedling difference in a kind of sedge morning of Radix Angelicae Sinensis, grower thinks, vernalization
Whether being the main cause causing Radix Angelicae Sinensis early a kind of sedge, the vernalization ability of big seedling is different, causes this species diversity
Reason.But it is about Radix Angelicae Sinensis vernalization and the research of condition thereof, extremely limited.The earliest about Radix Angelicae Sinensis treatment temperature
Research discloses, and 1～4 DEG C can promote Radix Angelicae Sinensis early a kind of sedge with storage at-4～-2 DEG C；Preserve at 10～13 DEG C, then
Early a kind of sedge rate is the lowest.This research temperature scale is relatively big, processes number of days and fails to understand, and with the storage of surviving the winter of medicinal herb grower's Radix Angelicae Sinensis Seedling
Deposit temperature conditions quite different, and be not inconsistent with the observation phenomenon of early stage, therefore the present invention to Radix Angelicae Sinensis vernalization temperature,
The process time does further investigation further, to disclose vernalization characteristic a kind of sedge reason early of Radix Angelicae Sinensis.
Radix Angelicae Sinensis (Angelica sinensis) is the plant with medical value of crucial importance, but Radix Angelicae Sinensis planting conditions is severe
Carve, planting area limited, limit its large-scale production.And Early bolting (being called for short " early a kind of sedge ") is to limit it to produce
One major reason of amount.
Summary of the invention
It is an object of the invention to provide a kind of reduce Radix Angelicae Sinensis early a kind of sedge rate store up seedling-growing method for a long time, it is intended to solve to work as
Return planting conditions harshness, planting area limited, the problem limiting its large-scale production.
The present invention is achieved in that the invention discloses a kind of described reduction Radix Angelicae Sinensis early a kind of sedge rate stores up Seedling for a long time
Method is stored in the temperature range of-5 DEG C～-15 DEG C；Regular opening and closing refrigerator doors ventilation during storage；Seedling
Storage can't use dry ground, must use wetland.
Further, described purging steps: open refrigerator doors, pull chamber door, work fan is vaporous agitates for several times, closes
Closing, in completing case, gas is changed.
Further, described wetland, anhydrous leaching when extruding with hands, and have wet trace for tide；Squeeze with hands
Pressure, accumulated water appears as wet.Moist i.e. between damp and wet between any ratio (note, manual operations cannot be concrete
To a certain ratio, as long as between this scope).
The method that the present invention provides is experimentally confirmed, the Seedling of storage at-5 DEG C, takes out after storing 1 year,
Still having good vitality, planting survival rate reaches 100%.Therefore, the temperature range that the present invention provides
Storage Radix Angelicae Sinensis Seedling i.e. advantageously reduces Radix Angelicae Sinensis early a kind of sedge rate, also can long-term storage.
Accompanying drawing explanation
Fig. 1 is that the bolting reaction of the Temperature Treatment that the embodiment of the present invention provides becomes positive correlation to show with the size of Radix Angelicae Sinensis
It is intended to.
Fig. 2 is that the embodiment of the present invention provides-5 DEG C, and 0 DEG C and 5 DEG C persistently processes Radix Angelicae Sinensis early a kind of sedge influence curve
Schematic diagram.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment,
The present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to
Explain the present invention, be not intended to limit the present invention.
Below in conjunction with test, the application principle of the present invention is further described.
1 materials and methods
1.1 test materials and confession examination soil
Test Radix Angelicae Sinensis Seedling used is " Minxian County Radix Angelicae Sinensis ", educates from Dongshan, Minxian County, Dingxi City, Gansu Province, height above sea level about 3000m
Nursery.Nursery was opened up wasteland by wild wasteland (not cultivating crop) and is formed.Reclamation of wasteland nursery is year
Accumulating the black earth (local person " hollow earth ") rich in humus for a long time, seed used by nursery is 3 years Radix Angelicae Sinensis institutes
Tie normal seed.Screening does not has the Radix Angelicae Sinensis Seedling of disease and mechanical damage to test, and in November, 2012 starts more
Winter K cryogenic treatment, is carried out from the Tianshui of Minxian County 300km, height above sea level about 1000m to Temperature Treatment, next year 3
The moon 23, taking back height above sea level 2300m under Dongshan, Minxian County, Dingxi City, Gansu Province, front stubble is that the farming land of Semen Viciae fabae stubble moves
Planting, agricultural geology is loessal soil.
The Temperature Treatment of 1.2 Radix Angelicae Sinensis Seedlings
1) classification of Radix Angelicae Sinensis Seedling
First results are divided into different stage from the Seedling of nursery by root diameter, use slide gauge to measure and return under head 1
Diameter at cm, all of young plant is divided into 3 classes: diameter is respectively smaller than 0.45cm, between 0.46-0.65cm
And between 0.66-0.85cm, within each class Radix Angelicae Sinensis retains the short 1cm of root length, the close Seedling of side radical, root length.
* long measure is cm, and the above-mentioned experiment measurement date is on November 14th, 2012.
2) Temperature Treatment
Temperature Treatment refrigerator is Aukma heterothermic refrigerator (model), and range of temperature is ± 1.Arrange-5 DEG C,
0 DEG C and 5 DEG C of three kinds of Temperature Treatment, Seedling different size of to annual Radix Angelicae Sinensis carries out the lasting process of three kinds of temperature,
On this basis, the regular conversion process between different temperatures is carried out.In conversion process, periodically (every 30d)
By the Radix Angelicae Sinensis Seedling under a kind of Temperature Treatment, continue with at a temperature of proceeding to other two kinds to transplanting, to create not
Synthermal and the integrated treatment of different number of days.Different disposal temperature and Seedling size is observed to Radix Angelicae Sinensis early a kind of sedge after transplanting
Impact, analyze the vernalization performance of Radix Angelicae Sinensis Seedling and difference.
Temperature Treatment starts from November 15th, 2012, and December carries out temperature transition for the first time on the 15th and processes,
On January 15th, 2013 is second time batch, and January 15 was third time batch, transplanted to February 15.?
In the temperature transition of every 30d, proceeded to the process material of 5 DEG C by-5 DEG C, or proceeded to the process of-5 DEG C by 5 DEG C,
7d transition adaptation is carried out at 0 DEG C of refrigerator before proceeding to.The conversion of adjacent temperature is then without transition.Total for carrying out
21 kinds of different temperatures and the combined treatment of number of days.Different size of Seedling is given every kind of concrete temperature by quantity
Process, with nursery soil seedling, tired prick after, be embedded in and contain in native box, together with little during regular temperature transition
Box shifts together.The Temperature Treatment of Seedling originates on November 22nd, 2012.Temperature Treatment period, 5 DEG C of Seedlings were wet
Degree is maintained at more than 80%.While processing Radix Angelicae Sinensis Seedling with refrigerator, transplant ground at Radix Angelicae Sinensis, arrange one the most right
According to, the winter Tibetan carrying out Seedling according to locality custom processes.
The plantation of 1.3 materials
Radix Angelicae Sinensis Seedling processes transplanted on March 22nd, 2013.Transplanting ground is under Dongshan, Minxian County, Dingxi City, Gansu Province
Height above sea level is the plot of 2300m.Chosen place elder generation stubble is Semen Viciae fabae, smooth after deep ploughing, and loosing soil is used
Base fertilizer is the farm manure that high-quality is become thoroughly decomposed.Plastic film is black 1m width.Transplanting method is by local habit of planting
Being used to carry out, seedlings use individual plant to plant, and seedling every 2 or 3 Seedling is a brood of.Community uses single factor test random district group
EXPERIMENTAL DESIGN, repeats for three times.Plantation spacing in the rows 30cm, line-spacing 40cm, aisle 40cm, surrounding protection row
More than 1m is wide.Mulch film is first stabbed into aperture (degree of depth in hole cannot be below the length of Seedling), so with scuppit by plantation
After vertically put Seedling into hole, the head of Seedling is slightly below mulch film, Seedling place Miao Houjia in right amount soil covering with plastic film
On cave eye, earthing thickness 2cm.Pushing mulch film in time aside after spire is unearthed and put Seedling, final-period management to be rooted out in time
Weeds.Fertilising and other management are with local field-transplanting custom.
2 results and analysis
2.1 3 kinds of constant temperature process the impact on Radix Angelicae Sinensis early a kind of sedge
First, for examination Radix Angelicae Sinensis respectively at-5 DEG C, persistently process at a temperature of 0 DEG C and 5 DEG C three kinds and survive the winter, to next year
Spring transplants.Result shows, under the lasting process of each temperature, and the Radix Angelicae Sinensis seedling of below diameter 0.45cm,
Bolting rate is extremely low, and between the process of between process and locality storage for the winter, difference is notable (P < 0.05) (table 1).Can
Seeing, Radix Angelicae Sinensis seedling does not have experiences thermal stimulus and vernalization ability, is i.e. in non-competence.And 0.46cm with
On relatively gigantic angelica Seedling, bolting, to treatment of different temperature, has different reactions.0 DEG C and 5 DEG C process, bright
Aobvious a kind of sedge morning promoting Radix Angelicae Sinensis seedlings, the Radix Angelicae Sinensis Seedling of 0.46-0.65cm is at a temperature of above-mentioned two kinds, and early a kind of sedge rate is divided
Not for reaching 32.4% and 20.8%, it is much higher than-5 DEG C and naturally preserve 2.5% and 3.6%.0.66-0.85cm
Radix Angelicae Sinensis 0 DEG C and 5 DEG C persistently process under a kind of sedge rate morning be respectively 73.1% and 32.4%, be much higher than-5 DEG C and
Naturally 2.7% and 17.1% (table 1) of storage.
Visible, Wintering Period 0 DEG C and 5 DEG C persistently processes, and has effectively facilitated diameter 0.46cm above Radix Angelicae Sinensis Seedling
Bolting, and the lasting process of-5 DEG C, it is shown that the bolting rate of extremely low level.Moreover, at 0 DEG C and 5 DEG C
Diameter 0.46cm above Radix Angelicae Sinensis Seedling under Li, and Miao Yue great, early a kind of sedge reaction the strongest, it is seen then that 0 DEG C and with
Upper temperature can effectively facilitate Radix Angelicae Sinensis spring flower bolting, and the bolting reaction of Temperature Treatment becomes positive correlation with the size of Radix Angelicae Sinensis
(Fig. 1), dependency reaches 0.998.
The lasting process of the table 1 different temperatures impact on Radix Angelicae Sinensis vernalization a kind of sedge early
The Seedling of § 0.35cm often processes 30 strains；The Seedling of 0.36-0.45cm processes 30 strains.Strain number is mean value ±
Standard error.
As it is shown in figure 1, dependency 0.998*；* significant correlation in 0.05 level.
The impact on Radix Angelicae Sinensis early a kind of sedge of the conversion process between 2.2 different temperatures
In above-mentioned experimental basis, for the vernalization performance of further clear and definite Radix Angelicae Sinensis Seedling, so that Temperature Treatment is failed to understand
The Radix Angelicae Sinensis seedling (0.35cm) felt and the Radix Angelicae Sinensis that constant temperature is processed the critical range with bolting reaction
Seedling (0.46～0.65cm) is material, carries out periodically shifting conversion between three kinds of temperature 0 DEG C ,-5 DEG C and 5 DEG C
Process, with Plant age and the vernalization number of days marginal value of clear and definite Radix Angelicae Sinensis, and the impact of temperature transition.
2.2.1 high temperature (5 DEG C) shifts the impact on Radix Angelicae Sinensis early a kind of sedge to low temperature (0 DEG C/-5 DEG C)
In the experience process in advance of 5 DEG C, after proceed to respectively process at 0 DEG C and-5 DEG C to transplanting.From a kind of sedge number morning
From the point of view of mesh, seedling (0.35cm), is not caused to the regular transfer processing of 0 DEG C He-5 DEG C by 5 DEG C
The early notable change of a kind of sedge rate, wherein, first through 5 DEG C of 90d pretreatment, by 0 DEG C of Radix Angelicae Sinensis seedling processed, early
A kind of sedge rate is the highest, reaches 8.4% (table 2), it is seen then that by 5 DEG C to 0 DEG C of regular transfer processing, can not cause little
Increasing substantially of Seedling Radix Angelicae Sinensis early a kind of sedge rate, but between each process, difference is notable (P ＜ 0.05), and this still shows,
Seedling Radix Angelicae Sinensis do not possess experience variations in temperature process and the ability of vernalization.And the Radix Angelicae Sinensis Seedling of (0.46～0.65cm)
By 5 DEG C of conversions processed in advance to 0 DEG C He-5 DEG C, bolting rate has had large increase, has naturally compareed with locality
Between significant difference (P ＜ 0.05), early a kind of sedge rate processes the prolongation of time in advance with 5 DEG C and improves, it is seen that 5 DEG C
Long-term disposal may advantageously facilitate Radix Angelicae Sinensis vernalization and early a kind of sedge, and high temperature storage of surviving the winter is prone to vernalization bolting.
Table 2 by 5 DEG C to 0 DEG C or the impact on Radix Angelicae Sinensis early a kind of sedge rate of-5 DEG C of transfer processing
The Seedling of § 0.35cm often processes 30 strains；The Seedling of 0.36-0.45cm processes 30 strains；Strain number is mean value ±
Standard error.
From the point of view of going out shoot survival percent after transplanting, 5 DEG C of process of refrigerator, greatly reduce the planting percent of Radix Angelicae Sinensis；5℃
With under the conversion process of low temperature, the survival rate of young plant is up to 70%, minimum reaches 35.5%.This may be with 0
Upper temperature preserves and causes part Seedling bacteria infection, after transplanting rotten, dead relevant.Therefore, the temperature of Radix Angelicae Sinensis
Treatment research, especially notes the preservation of young plant, reduces loss.
2.2.2 0 DEG C processes backward 5 DEG C and-5 DEG C transfers impact on Radix Angelicae Sinensis early a kind of sedge
The Radix Angelicae Sinensis seedling of below diameter 0.35cm, processes in 0 DEG C of early stage of experience, more periodically proceeds to 5 DEG C and-5 DEG C
Under process, bolting rate is extremely low, still the highest with 0 DEG C of bolting rate persistently processed, and reaches 4.9%, but each other
Difference is not notable (P ＜ 0.05).Visible, 0 DEG C of early stage of experience is processed by Radix Angelicae Sinensis seedling, then continues at other temperature
The process of degree is insensitive, again shows that, the Plant age that temperature inversion is processed by seedling Radix Angelicae Sinensis is insensitive.So
And, diameter, between the relatively gigantic angelica Seedling of 0.46-0.65cm, shows significantly higher bolting rate.By 0 DEG C
To the transfer processing of 5 DEG C, proceeding to the process of 5 DEG C after processing 30d with 0 DEG C, bolting rate is the highest, reaches 27.3%；
Processing reducing and the increase of 5 DEG C of process times of time with 0 DEG C, bolting rate gradually steps up.As can be seen here, 0 DEG C
Early stage processes and 5 DEG C of process subsequently, processes the increasing the minimizing with 5 DEG C of natural law of number of days with 0 DEG C and improves.
By 0 DEG C of freezing processing to-5 DEG C of transfers, the impact on bolting is relatively low, the lasting place being significantly less than at 0 DEG C
Reason, wherein, with 0 DEG C of 30d, the freezing processing of latter-5 DEG C, bolting rate is minimum, reaches 5.4%, with but 0 DEG C
The prolongation of process time, bolting rate has increase trend (table 3).Visible, 0 DEG C and 5 DEG C of combined treatment, to rush
The vernalization bolting entering Radix Angelicae Sinensis seedlings is more effective.
Table 3 by 0 DEG C to 5 DEG C or the impact on Radix Angelicae Sinensis early a kind of sedge rate of-5 DEG C of transfer processing
The Seedling of § 0.35cm often processes 30 strains；The Seedling of 0.36-0.45cm processes 30 strains.Strain number is mean value ±
Standard error.
2.2.3 by-5 DEG C of pretreatment to impact on Radix Angelicae Sinensis early a kind of sedge of the temperature transfer of 0 DEG C or 5 DEG C
Experience the early stage process of-5 DEG C, respectively at going to 0 DEG C and the process of 5 DEG C, wherein, below 0.35cm
Radix Angelicae Sinensis seedling, bolting rate extremely low (table 4), and between different disposal, difference is the most notable.Visible, before-5 DEG C
Phase processes backward 0 DEG C and the process of 5 DEG C, can not promote the bolting of below 0.35cm Radix Angelicae Sinensis seedling equally, then
Secondary explanation, seedling Radix Angelicae Sinensis does not have the ability experiencing K cryogenic treatment and vernalization.But root diameter is between 0.46-0.65
The seedlings Radix Angelicae Sinensis of cm, after experiencing this process, bolting rate is greatly improved, and there are differences between different number of days process.
Proceeding to the process of 0 DEG C after experiencing-5 DEG C of 30d, bolting rate is higher than the lasting process of-5 DEG C and compares, and warp
Go through-5 DEG C of 33d, 83d early stages to process, after proceed to the process of 0 DEG C, bolting rate reduces successively, bolting rate with
0 DEG C and 5 DEG C processes the increase of number of days and improves, and i.e.-5 DEG C freezings pretreated 0 DEG C and 5 DEG C of process can have
Effect promotes Radix Angelicae Sinensis bolting, and bolting rate becomes positive correlation (table 4) with 0 DEG C and 5 DEG C of cold treatment numbers of days.
Table 4 by-5 DEG C to 0 DEG C or the impact on Radix Angelicae Sinensis early a kind of sedge rate of 5 DEG C of transfer processing
The Seedling of § 0.35cm often processes 36 strains；Strain number is mean value ± standard error.
-5 DEG C of early stages of the different number of days of Radix Angelicae Sinensis experience process, and proceed to 5 DEG C and the process of 0 DEG C, to 0.35cm with
The ahead of time bolting of interior Radix Angelicae Sinensis Seedling does not affect, thus, the most freezing, after carry out the high-temperature process thawed, equally
Not affecting the ahead of time bolting rate of seedling, illustrate, seedling Radix Angelicae Sinensis does not have a perception low temperature stimulation and the ability of vernalization,
I.e. it is in non-competence.
Existing result of the test:
| Storage temperature | -25℃ | -18～-16 DEG C |
| Survival rate | 0% | 0% |
According to above result of the test, in conjunction with forefathers result of study (Wang Wenjie, 1968；1972), will the most specially
The temperature range of profit protection is limited between-15 DEG C to-5 DEG C.
This test sets 5 DEG C, and 0 DEG C and-5 DEG C of three temperature, to simulate the annual Radix Angelicae Sinensis Seedling that grows directly from seeds, storage of surviving the winter
The temperature being likely encountered during Tibetan, respectively high temperature in simulation 0, critical temperature 0 DEG C and-5 DEG C of freezing state,
The latter is higher than the minimum temperature (-7--8 DEG C) of the Wintering Period storage of Radix Angelicae Sinensis.Visible the selected temperature 5 DEG C of test,
0 DEG C ,-5 DEG C, substantially from the point of view of within the scope of selected temperature is positioned at medicinal herb grower's storage temperature fluctuation, but minimum with the highest
Temperature difference is relatively big (medicinal herb grower's estimation).When being classified as low temperature long-day plant, after receiving K cryogenic treatment,
Bolting is bloomed and is often also needed to the illumination of certain time.
Visible, 0 DEG C and the storage of temperatures above, effectively facilitate the vernalization bolting of Radix Angelicae Sinensis, and-5 DEG C of freezings
Under process, then suppression Radix Angelicae Sinensis bolting as shown in Figure 2.By above-mentioned test, preliminary announcement is more following asks
Topic:
(1). the lasting process of each temperature :-5,0 and 5 DEG C of three temperature will be placed in by different size of Radix Angelicae Sinensis Seedling
Under degree, Wintering Period persistently processes, and transplants to next year.Result shows, lasting process early a kind of sedge rate of 0 DEG C is the highest,
0 DEG C of process is more beneficial for promoting the vernalization bolting of Radix Angelicae Sinensis, especially seedlings；5 DEG C persistently process take second place；At-5 DEG C
Storage, early a kind of sedge rate is minimum.-5 DEG C process and make owing to temperature is too low Radix Angelicae Sinensis Seedling be nearly at hibernation-like state, therefore its
Physiological activity temporarily tends to stop, thus Wintering Period m-5 DEG C process under Radix Angelicae Sinensis Seedling failed vernalization, or
Say the condition failing possess bolting.
Through the Wintering Period temperature of this test, process and be found to have rotten Seedling phenomenon, especially with the lasting place of 5 DEG C when transplanting
Reason is the most serious, and the basic of Radix Angelicae Sinensis is easily rotted by microbial infection, causes actual plantation strain number to compare temperature
Reduce before processing；Moreover, experience 5 DEG C persistently process transplant time serious phenomenon of emerging, bud elder occur
Reach 4,5cm.Wintering Period there is also same with 4 DEG C of process, and they wipe out before transplanting and sprout.Radix Angelicae Sinensis Seedling goes out
Bud and the person that enters early development have had a strong impact on the early stage after it is transplanted and have emerged.This point from emergence rate it can be seen that
Emergence rate 0 DEG C process is the highest, and-5 DEG C lower slightly, and the two difference is not notable, and worst (P > 0.05) is emerged in 5 DEG C of process.
This part Radix Angelicae Sinensis is easily affected by cold spell in spring in early days etc. after transplanting and fails to grow further, causes the big of emergence rate
Amount loss, the Seedling that this partial loss is fallen, then may complete vernalization, possess bolting ability, sprout weak or not
Viability of sprouting is strong and emerges, and this part does not then obtain fine vernalization, and this phenomenon explains 5 DEG C well and holds
The continuous Seedling early a kind of sedge rate that processes is significantly less than 0 DEG C of anomalism persistently processed.Store about at Wintering Period 10～13 DEG C
Hide the conclusion that early a kind of sedge rate is the lowest, its reason be that grow the vernalization that sprouts can early a kind of sedge Seedling, cannot be just after transplanting
Chang Chengmiao, thus reduce a kind of sedge rate morning of this Temperature Treatment.To this end, relevant process of the present invention 5 DEG C suitably increases
Seedling number.
(2). the Radix Angelicae Sinensis large and small Seedling difference reaction to 0 DEG C of storage conversion process.It can be seen that generally, 0 DEG C
A kind of sedge rate morning persistently processing comparison is higher than the conversion process of 0 DEG C and other temperature；0 DEG C to high temperature 5 DEG C and low temperature
Transfer, do not affect vernalization early a kind of sedge of seedling (0.46～0.65).0 DEG C at the transfer of high temperature 5 DEG C and low temperature
Reason, has different impacts to a kind of sedges morning of seedlings, wherein, 0 DEG C to the transfer of-5 DEG C, to promoting Radix Angelicae Sinensis vernalization
Early a kind of sedge is most effective, and effect is even better than the lasting process of 0 DEG C.
(3). the reaction to the conversion process of different temperatures of the Radix Angelicae Sinensis seedling:
<1>. identical temperature (conversion) processes, and seedlings are higher than seedling vernalization bolting rate.With the increase of young plant,
Early a kind of sedge probability increases, the transfer processing (-5 DEG C to 5 DEG C, centre carries out the transition of 0 DEG C) of portion temperature, place
Reason time and bolting rate present obvious positive correlation.
<2>. the conversion process between different temperatures, seedling bolting is not affected, therefore, Radix Angelicae Sinensis seedling does not has
There is the ability experiencing low temperature stimulation and vernalization；I.e. the same with most of fruit tree kinds, there is a childhood, only
But fruit tree kind is before completing childhood, is in strict not bloom；And seedling Radix Angelicae Sinensis the most also has Seedling
Sub-bolting, it appears that unlike fruit tree kind is so strict, this preservation being probably Radix Angelicae Sinensis organ of surviving the winter is root, Radix Angelicae Sinensis
A kind of sedge morning affected by mechanical wounding, microbial activities etc..
<3>. treatment of different temperature, all can effectively facilitate the bolting of seedlings, illustrate that seedlings are sensitive to Temperature Treatment.
<4>.5 DEG C is shifted-5 DEG C, favourable to suppression Radix Angelicae Sinensis bolting；
<5>. by freezing state to the transfer of high temperature (0 and 5 DEG C), may advantageously facilitate the vernalization bolting of Radix Angelicae Sinensis；Its
In, to the transfer of 0 DEG C, effect is weaker than the transfer to 5 DEG C.To the process time shifting each temperature of 0 DEG C,
The most notable on bolting impact；To the transfer of 5 DEG C, early a kind of sedge rate becomes negative correlation with freezing processing number of days, at high temperature
Reason number of days becomes positive correlation.
3.2 K cryogenic treatment numbers of days and conversion process temperature and test correlation analysis
In low-temperature process, set to each treatment temperature and process number of days accordingly, Er Qie
Can conversion process temperature after processing a period of time.Three selected in test Temperature Treatment have takes out in early days
A kind of sedge plant, it was demonstrated that these three temperature all can induce Radix Angelicae Sinensis Seedling early a kind of sedge；And the most different process
Time, under each process number of days, early a kind of sedge rate was roughly the same, does not has notable difference on a kind of sedge rate impact display morning；And
Under same initial temperature, no matter temperature through having transformed to which temperature is after a period of time carried out, early a kind of sedge rate
Also without significant difference, it is possible to determine, in this test after three initial temperatures process 23 days
A kind of sedge situation morning of Seedling is basicly stable, after transformation temperature and corresponding to process the time less on a kind of sedge rate impact morning.
The reaction to Temperature Treatment of the 3.3 Seedling sizes
Miao Yue a kind of sedge rate very early in the morning is the highest, and≤0.45cm, it is right for the Radix Angelicae Sinensis Seedling of 0.46-0.65cm, 0.66-0.85cm
A kind of sedge rate morning answered is respectively 1.92%, 11.01%, 78.26%.The Radix Angelicae Sinensis Seedling of 0.66-0.85cm scope early a kind of sedge rate
Up to 78.26%, the yield of Radix Angelicae Sinensis is caused and has a strong impact on by this, and the Radix Angelicae Sinensis Seedling of≤0.45cm early a kind of sedge rate is
1.92%, in terms of morning a kind of sedge rate angle, the Seedling of≤0.45cm should be optimum selection.Comprehensive with early a kind of sedge rate to emergence rate
Though the Seedling of analysis≤0.45cm early a kind of sedge rate is low, but emergence rate is only 47.87%, does not also reach half；
Though the Radix Angelicae Sinensis Seedling emergence rate up to 83.78% of 0.66-0.85cm, its early a kind of sedge rate reached 78.26%；
The Seedling emergence rate of 0.46-0.65cm is 77.99%, and early a kind of sedge rate is 11.01%.It is not difficult to find out from this, at Radix Angelicae Sinensis
In production process, the Seedling of 0.46-0.65cm is only optimum selection (optimum selection utilizes Juanjuan ZHANG data analysis).
Radix Angelicae Sinensis there will be a certain proportion of bolting plant after accepting K cryogenic treatment, the viewpoint generally admitted
Being exactly through low temperature induction, part Radix Angelicae Sinensis Seedling completes vernalization process.
The relation of Seedling size and early a kind of sedge rate
Radix Angelicae Sinensis Seedling a kind of sedge rate the least morning is the lowest, and Seedling a kind of sedge rate the most very early in the morning is the highest, it is seen then that the different size of Seedling perception spring
The ability changing Temperature Treatment is different, only completes the plant of vernalization process, could bloom by bolting.Diameter >=0.45cm
And following Radix Angelicae Sinensis seedling, do not possess or the ability of few perception vernalization Temperature Treatment, and fail early
A kind of sedge.Diameter has possessed the ability of perception low temperature vernalization at 0.46cm and above Radix Angelicae Sinensis Seedling thereof, and with the increasing of Seedling
Big ability gradually strengthens, and presents Seedling vernalization ability and becomes positive correlation with Radix Angelicae Sinensis size, but its size neither one is bright
True vernalization marginal value；On 0 DEG C or 0, the vernalization of temperature early a kind of sedge becomes positive correlation with the time of process.Visible, Radix Angelicae Sinensis
Vernalization be that a nursery size becomes positively related quantitative trait.Thus speculating, nursery ahead of time, prolongation are educated
The Radix Angelicae Sinensis Seedling that Seedling time gained is bigger only needs of short duration K cryogenic treatment or possesses without vernalization at all.
Both comprehensive ,-5 DEG C not only can ensure that emergence rate but also can reduce early a kind of sedge rate to a certain extent, it is seen then that
5 DEG C, 0 DEG C, the temperature of surviving the winter that at-5 DEG C, Seedling is optimal should be-5 DEG C ,-5 DEG C process do not exist Seedling sprout in advance or
Grow tender shoots and affect the situation of emergence rate,.In reality is cultivated, storage for the winter especially storage for the winter in early days or
Next year gives freezing K cryogenic treatment before transplanting all the time, and raising and a kind of sedge rate morning to emergence rate have ideal
Result.
The present invention draws, reduces early a kind of sedge rate by the size and storage temperature of surviving the winter controlling Seedling, improves Radix Angelicae Sinensis
Yield, this conclusion can effectively instruct Radix Angelicae Sinensis to produce.
By this test it follows that the emergence rate after Radix Angelicae Sinensis is transplanted by the size of<1>Radix Angelicae Sinensis Seedling has the biggest shadow
Ring.The biggest emergence rate of Seedling is the highest, and the least emergence rate is the lowest, and both relations are positive correlations.<2>Radix Angelicae Sinensis
A kind of sedge rate impact morning after Radix Angelicae Sinensis is transplanted by the size of Seedling is obvious, and Radix Angelicae Sinensis Seedling a kind of sedge rate the most very early in the morning is the highest, and Miao Yue little is early
A kind of sedge rate is the lowest, and both are positive correlations.<3>under Different hypothermia processes, 0 DEG C early a kind of sedge rate the highest, 5 DEG C are taken second place,
-5 DEG C early a kind of sedge rate minimum.<4>test in treatment temperature midway change and process number of days difference to morning a kind of sedge rate and
Emergence rate does not significantly affect.In production, the storage of 0 DEG C and temperatures above is survived the winter, the easy vernalization of Radix Angelicae Sinensis Seedling,
Cause early a kind of sedge；Can be using the chilled storage of less than 0 DEG C as the method reducing Radix Angelicae Sinensis early a kind of sedge rate.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this
Any amendment, equivalent and the improvement etc. made within bright spirit and principle, should be included in the present invention
Protection domain within.
Claims (3)
1. one kind reduce Radix Angelicae Sinensis early a kind of sedge rate store up seedling-growing method for a long time, it is characterised in that described reduction Radix Angelicae Sinensis early a kind of sedge
The long-term storage seedling-growing method of rate is stored in the temperature range of-5 DEG C～-15 DEG C；Regular opening and closing refrigerator during storage
Valve scavenging；The storage of Seedling can't use dry ground, must use wetland.
2. the Radix Angelicae Sinensis early a kind of sedge rate that reduces described in claim 1 store up seedling-growing method for a long time, it is characterised in that described
Purging steps: open refrigerator doors, pulls chamber door, and work fan is vaporous agitates for several times, closes, completes gas in case
Change；Or Special scavenging class device completes gas replacing in case.
3. the Radix Angelicae Sinensis early a kind of sedge rate that reduces described in claim 1 store up seedling-growing method for a long time, it is characterised in that described
Wetland, anhydrous leaching when extruding with hands, there is wet trace for tide；Extruding with hands, accumulated water appears as wet；
Moist i.e. between damp and wet between arbitrarily than.
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| CN112425706A (en) * | 2020-11-30 | 2021-03-02 | 天水师范学院 | Method for treating waste by utilizing microbial fermentation |
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| CN104429427A (en) * | 2013-09-23 | 2015-03-25 | 方子森 | Planting method for preventing and treating early bolting of Angelica sinensis |
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