CN1063012C - Artificial seed - Google Patents
Artificial seed Download PDFInfo
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- CN1063012C CN1063012C CN96103270A CN96103270A CN1063012C CN 1063012 C CN1063012 C CN 1063012C CN 96103270 A CN96103270 A CN 96103270A CN 96103270 A CN96103270 A CN 96103270A CN 1063012 C CN1063012 C CN 1063012C
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- Prior art keywords
- artificial
- calcium alginate
- endosperm
- artificial seed
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- Pretreatment Of Seeds And Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to an artificial seed which comprises an adventitious embryo or an adventitious bud, artificial endosperm and a seed coat, wherein the artificial endosperm contains microporous calcium alginate as a filling material of biodegradable macromolecular cysts, and the seed coat is a block copolymer of biodegradable polycaprolactone polyether. The artificial seed has the advantages of easy germination of embryo buds, simple preparation technology and low cost,and a bud ratio is 70%.
Description
The present invention relates to a kind of artificial seed technology, specially refer to the artificial seed field of alginates of adopting as artificial endosperm matrix.Artificial seed is a kind of technology that proposes the latter stage seventies.Usually; the artificial seed core is by the adventitious embryo of embedding (bud); the intermediate layer is the artificial endosperm that contains long desired nutritional composition of adventitious embryo blastogenesis and plant growth regulator; outermost layer is the kind skin of being made up of macromolecule membrane, avoids the impact of losing and preventing outside insect pest and physics strength in order to the protection contained humidity.The artificial seed that similar functions is arranged by a kind of and natural seed of being combined to form of three parts.
Clear and 60-214811 and clear and 63-5009111 have reported and have added water-absorbing material such as water-absorbing resins or active carbon to improve water retention capacity in the artificial endosperm of alginate matrices.But this technology can make the endosperm gel strength improve and be unfavorable for the plumule sprouting.In addition, the viscosity of alginate jelly can cause inter-adhesive between artificial seed, is unfavorable for carrying out the mechanization sowing.
Also reported in the above-mentioned document and adopted high-temperature sterilization and add the antiseptic power of the method for antibiotic, insecticide with the raising artificial endosperm, but because operating process needs to carry out under aseptic condition, make its complex process, cost improves, and particularly high-temperature sterilization will cause the molecular weight and molecular weight and the quality instability of alginates.In addition, because general macromolecule membrane can natural degradation, intensity can not change, and hinders bigger to seed germination during as kind of skin.
The present invention is in order to overcome complex process in the artificial seed preparation, the cost height, plumule is sprouted slow, and bud ratio is low, and the easy shortcoming of adhesion and provide a kind of and contain the artificial endosperm of little porous aggregate and have the artificial seed of the macromolecule membrane of biodegradability as kind of degree between artificial seed.Utilize the present invention also can not need through autoclaving and do not need aseptic operating condition, under general clean and tidy laboratory condition, operate getting final product, thereby further simplifies the preparation condition of artificial seed and the preparation cost of reduction artificial seed.
The adventitious embryo (bud) of the core of artificial seed of the present invention for getting by Plant Tissue Breeding, adventitious embryo (bud) is by containing little porous aggregate, the MS medium, the alginates artificial endosperm parcel of biological sterilization agent and plant growth regulator, outermost kind of skin is the Biodegradable polycaprolactone polyether block polymer film, and this membrane action is to slow down the forfeiture of nutrient and moisture and prevent between insect pest, foreign impacts and artificial seed inter-adhesive.
Above-mentioned artificial endosperm comprises following component and content:
Constituent content (weight portion)
Mosanom 1-8
Treated little porous aggregate 10
-2～2 are preferably 5 * 10
-2～1
MS medium 100
Biological sterilization agent 10
-4～10
-1Be preferably 5 * 10
-3～10
-2
Plant growth regulator 10
-6～10
-4
Above-mentioned little porous aggregate is the calcium alginate grain of handling through the Biodegradable high molecular packing, and it and alginate jelly affinity are good.Because the inside and outside wall of micropore all has one deck biodegradation high molecular as slow release layer, can make the moisture, the nutrient that comprise in it pass through the slow release layer sustained release.Because the existence of little porous aggregate also can make the intensity of alginate jelly reduce, and makes embryo (bud) be easy to sprout.Micropore contains air, can supply the needs of embryo (bud) growth.
Described MS medium is the normal structure medium that contains mineral salt and carbohydrate.Bactericidal agent is carbendazim, streptomycin, sorbic acid, actinomycin, captan, aspirin or its compound.Plant growth regulator is indolebutyric acid, naphthalene diacid, 2, and 4-D, kinetin, 6-benzyl purine, zeatin etc.
The method for making of above-mentioned artificial endosperm is with mosanom 1-8 part, treated little porous calcium alginate grain 10
-2～2 parts, biological sterilization agent 10
-4～10
-1Part, plant growth regulator 1,000,000/to ten thousand/portion, inject the container that fills 100 parts of MS medium under high-speed stirred stirred 1-5 minute, obtained the artificial endosperm matrix solution.
Artificial seed method for making of the present invention is that adventitious embryo or indefinite bud are mixed with the artificial endosperm matrix solution, splashing into concentration expressed in percentage by weight with dropper is the divalence beyond the demagging of 1-5% or the chloride solution of the above metal of divalence, placed 10-30 minute under stirring at a slow speed, solution of sodium alginate becomes alginate jelly.With the rinsing of MS medium after 1-2 minute, the alginate jelly ball that will comprise adventitious embryo or indefinite bud takes out, it is to soak for 10 seconds in the polycaprolactone polyether block polymer chloroformic solution of 1-10% (weight) in concentration that the back is blotted with filter paper in the surface, takes out and treats promptly to make artificial seed after the solvent evaporates.
Plant that the present invention is suitable for and crop varieties can be that eucalyptus, wheat, rape, Chinese cabbage, mung bean, logical tawny daylily, jonquil etc. are woody, draft, beans, vegetables and cereal crops kind.The growth object can be adventitious embryo or indefinite bud.
The adventitious embryo or the bud of artificial seed of the present invention are easily sprouted, and bud ratio can reach 70%, adopt the endosperm that contains little porous aggregate can keep moisture, nutrient, make embryo (bud) be easy to sprout, also can play the oxygen supply effect, simultaneously mutual adhesion between artificial seed, preparation technology is simple, and conditional request is low, and cost is low.
Embodiment 1,
Get mosanom 2.5 grams, through little porous calcium alginate grain 0.5 gram that biodegradation high molecular was handled, biological sterilization agent 0.1 gram, 0.01 milligram of plant growth regulator after mixing, injects 100 gram MS medium under high-speed stirred, stirred 3 minutes, and made the artificial endosperm matrix solution.After will leading to the segment of tawny daylily stem and above-mentioned artificial endosperm matrix solution mixing, splash into dropper in the calcium chloride solution of 3% (weight), under stirring at a slow speed, placed 10 minutes.Use the rinsing of MS medium after 2 minutes again, calcium alginate gel ball that will parcel stem segment takes out, and blot on the surface, is 10 seconds of dipping in 2% the polycaprolactone polyether block polymer chloroformic solution in concentration, takes out the back solvent flashing, obtains logical tawny daylily artificial seed.
Embodiment 2,
Adventitious embryo (bud) is long-lived scape segment, and other method for making makes the jonquil artificial seed with embodiment 1.
Claims (5)
1, a kind of artificial seed comprises adventitious embryo or indefinite bud, endosperm and kind skin, it is characterized in that described adventitious embryo or indefinite bud are by containing filler, the MS medium, the calcium alginate artificial endosperm parcel of bactericidal agent and plant growth substance, outermost kind of skin is the Biodegradable polycaprolactone polyether block polymer film; Filler is the calcium alginate grain of handling through the Biodegradable high molecular parcel, and described adventitious embryo or indefinite bud are logical tawny daylily stem segment or eucalyptus bud embryo or long-lived scape segment.
2, artificial seed according to claim 1 is characterized in that described endosperm comprises the weight portion of following component and content
Constituent content
Mosanom 1-8
Handle 10 through biodegradation high molecular
-2～2
The calcium alginate grain
MS medium 100
Bactericidal agent 10
-4～10-1
Plant growth regulator 10
-6～10-4
3, artificial seed according to claim 2 is characterized in that described calcium alginate grain is 5 * 10 by weight
-2-1.
4, artificial seed according to claim 2 is characterized in that described bactericidal agent is 5 * 10 by weight
-3～10
-2
5, a kind of method for making of artificial seed is characterized in that carrying out in the following order:
(1) method for making of artificial endosperm matrix is with mosanom 1-8 part by weight, the calcium alginate grain of handling through Biodegradable high molecular 10
-2～2 parts, bactericidal agent 10
-6～10
-4, under agitation inject the container that fills 100 parts of MS medium, stirred 1-5 minute, obtain the artificial endosperm matrix solution,
(2) adventitious embryo or indefinite bud are mixed with the artificial endosperm matrix solution, splash in the calcium chloride solution that concentration expressed in percentage by weight is 1-5% with dropper, stirred 10-30 minute, calcium alginate solution becomes the calcium alginate gel ball, with the rinsing of MS medium after 1-2 minute, the calcium alginate gel ball that will comprise adventitious embryo or indefinite bud takes out, and the surface is blotted the back with filter paper and soaked for 10 seconds in weight concentration is the polycaprolactone polyether block polymer chloroformic solution of 1-10%, solvent evaporates is treated in taking-up, obtains artificial seed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96103270A CN1063012C (en) | 1996-03-15 | 1996-03-15 | Artificial seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96103270A CN1063012C (en) | 1996-03-15 | 1996-03-15 | Artificial seed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1148463A CN1148463A (en) | 1997-04-30 |
| CN1063012C true CN1063012C (en) | 2001-03-14 |
Family
ID=5117951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96103270A Expired - Fee Related CN1063012C (en) | 1996-03-15 | 1996-03-15 | Artificial seed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1063012C (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1080744C (en) * | 1997-09-25 | 2002-03-13 | 中国科学院化学研究所 | Biodegradation high molecular composition and its preparing method and use |
| CN101861832B (en) * | 2001-06-28 | 2013-03-27 | 森下仁丹株式会社 | Artificial seed |
| CN100403883C (en) * | 2006-05-11 | 2008-07-23 | 南京大学 | Anoectochilus formosanus Hayata artificial seed preparation method |
| CN100407905C (en) * | 2006-05-11 | 2008-08-06 | 南京大学 | Cremastra appendiculata(D.Don)Makino artificial seed preparation method |
| CN104798498B (en) * | 2015-04-29 | 2016-08-17 | 广西大学 | A kind of preparation method of Chinese Fir Plantation seed |
| CN105850736B (en) * | 2016-04-11 | 2017-11-10 | 中南林业科技大学 | A kind of preparation method of thizoma curculiginis artificial seed |
| CN108129197A (en) * | 2017-12-23 | 2018-06-08 | 吉林省农业科学院 | A kind of preparation method of the special drought-resistance seed-soaking agent of corn |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH034706A (en) * | 1989-05-31 | 1991-01-10 | Kubota Corp | Preparation of artificial seed |
| JPH03127920A (en) * | 1989-10-11 | 1991-05-31 | Kubota Corp | Preparation of artificial seed |
| JPH03206825A (en) * | 1990-01-08 | 1991-09-10 | Kubota Corp | Artificial seed and production thereof |
| JPH03285624A (en) * | 1990-03-30 | 1991-12-16 | Pentel Kk | Cultivation of plant tissue and artificial seed |
| WO1993003069A1 (en) * | 1991-08-07 | 1993-02-18 | Henkel Kommanditgesellschaft Auf Aktien | Polyacrylates and their use as levelling agents for powder coatings |
| EP0605721A1 (en) * | 1991-08-20 | 1994-07-13 | Kirin Beer Kabushiki Kaisha | Self-dehiscent artificial seed |
-
1996
- 1996-03-15 CN CN96103270A patent/CN1063012C/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH034706A (en) * | 1989-05-31 | 1991-01-10 | Kubota Corp | Preparation of artificial seed |
| JPH03127920A (en) * | 1989-10-11 | 1991-05-31 | Kubota Corp | Preparation of artificial seed |
| JPH03206825A (en) * | 1990-01-08 | 1991-09-10 | Kubota Corp | Artificial seed and production thereof |
| JPH03285624A (en) * | 1990-03-30 | 1991-12-16 | Pentel Kk | Cultivation of plant tissue and artificial seed |
| WO1993003069A1 (en) * | 1991-08-07 | 1993-02-18 | Henkel Kommanditgesellschaft Auf Aktien | Polyacrylates and their use as levelling agents for powder coatings |
| EP0605721A1 (en) * | 1991-08-20 | 1994-07-13 | Kirin Beer Kabushiki Kaisha | Self-dehiscent artificial seed |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1148463A (en) | 1997-04-30 |
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