JPH03127920A - Preparation of artificial seed - Google Patents
Preparation of artificial seedInfo
- Publication number
- JPH03127920A JPH03127920A JP26453989A JP26453989A JPH03127920A JP H03127920 A JPH03127920 A JP H03127920A JP 26453989 A JP26453989 A JP 26453989A JP 26453989 A JP26453989 A JP 26453989A JP H03127920 A JPH03127920 A JP H03127920A
- Authority
- JP
- Japan
- Prior art keywords
- meristem
- undifferentiated
- capsules
- artificial seeds
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002775 capsule Substances 0.000 claims abstract description 15
- 230000001939 inductive effect Effects 0.000 claims abstract description 5
- 230000004069 differentiation Effects 0.000 claims description 4
- 230000002062 proliferating effect Effects 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 8
- 230000002950 deficient Effects 0.000 abstract description 3
- 239000011259 mixed solution Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 abstract description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 abstract description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract description 2
- 229930006000 Sucrose Natural products 0.000 abstract description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 2
- 239000000648 calcium alginate Substances 0.000 abstract description 2
- 235000010410 calcium alginate Nutrition 0.000 abstract description 2
- 229960002681 calcium alginate Drugs 0.000 abstract description 2
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 abstract description 2
- 210000000056 organ Anatomy 0.000 abstract description 2
- 239000000661 sodium alginate Substances 0.000 abstract description 2
- 235000010413 sodium alginate Nutrition 0.000 abstract description 2
- 229940005550 sodium alginate Drugs 0.000 abstract description 2
- 239000005720 sucrose Substances 0.000 abstract description 2
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 239000001110 calcium chloride Substances 0.000 abstract 1
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 1
- 238000000034 method Methods 0.000 description 16
- 238000005538 encapsulation Methods 0.000 description 7
- 241000209094 Oryza Species 0.000 description 6
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 206010020649 Hyperkeratosis Diseases 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000003349 gelling agent Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000000442 meristematic effect Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 239000005972 6-Benzyladenine Substances 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000032459 dedifferentiation Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野）
本発明は、未分化な分裂組織から同一の形質をもった多
数の植物体を得るための人工種子の作成方法に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for producing artificial seeds for obtaining a large number of plants with the same traits from undifferentiated meristems.
従来、広く行われていた人工種子の作成方法としては、
第４図に示すように、植物体の一部をあらかじめカルス
培養や細胞感温培養等により未分化な分裂組織を培地内
で一定の大きさまで増殖しておき、その後、植物ホルモ
ン等で再分化させて幼植物体に誘導した後、寒天やアガ
ロース等でできたカプセルで包むことで人工種子化を行
っていた。Conventionally, the methods of creating artificial seeds that have been widely used include:
As shown in Figure 4, undifferentiated meristems of a part of the plant are grown in a culture medium to a certain size by callus culture or cell temperature-sensitive culture, and then redifferentiated using plant hormones, etc. After the seedlings were induced into seedlings, they were wrapped in capsules made of agar or agarose to create artificial seeds.
〔発明が解決しようとする課８］
上記方法によると、未分化な分裂組織の再分化を行った
後カプセル化処理を行い人工種子化するため、正常な幼
植物体のみを選別して人工種子化でき、効率的ではある
が、上記幼植物体は非常に傷つき易く、傷ついた幼植物
体は死滅するためカプセル化処理を慎重に行う必要があ
り、また、カプセル化処理は熟練を要するものであった
。そして、上記カプセル化処理工程での不良品の発生率
も高かった。[Problem 8 to be solved by the invention] According to the above method, since undifferentiated meristems are redifferentiated and then encapsulated to produce artificial seeds, only normal seedlings are selected and artificial seeds are produced. However, the above-mentioned seedlings are very easily damaged, and damaged seedlings will die, so the encapsulation process must be carried out carefully, and the encapsulation process requires skill. there were. Furthermore, the incidence of defective products in the encapsulation process was also high.
本発明の目的は、未分化な分裂組織からの人工種子の作
成方法を改良し、上記不具合を改善することにある。An object of the present invention is to improve the method for creating artificial seeds from undifferentiated meristems and to improve the above-mentioned problems.
〔課題を解決するための手段］
上記目的を達成するために、本発明においては、未分化
な分裂ｍ織を増殖させ、これを前記分裂組織が増−励可
能なカプセルで包み、その後前記カプセル内で分化誘導
して人工種子を作成した。[Means for Solving the Problems] In order to achieve the above object, the present invention proliferates undifferentiated meristem tissue, wraps it in a capsule in which the meristematic tissue can be stimulated, and then encloses it in a capsule capable of promoting the meristem tissue. Artificial seeds were created by inducing differentiation within the plant.
未分化な分裂組織の細胞塊においては、無傷の細胞が一
定数残存している限り組織の一部に損傷を与えても増殖
可能であるため、未分化な分裂組織に対する操作は比較
的容易である。つまり、上記方法によるとカプセル化処
理を増殖後の未分化な分裂Ｍｉ織の段階で行っているた
めカプセル化処理操作は容易に行うことができ、かつ、
熟練を要しない。As long as a certain number of intact cells remain in a cell mass of undifferentiated meristem, it is possible to proliferate even if a part of the tissue is damaged, so operations on undifferentiated meristem are relatively easy. be. In other words, according to the above method, the encapsulation process is performed at the stage of undifferentiated divided Mi tissue after proliferation, so the encapsulation process can be easily performed, and
No skill required.
従って、上記方法によると、人工種子の作成の操作を簡
素化でき、また、従来法に比べ人工種子の操作過程での
不良品の発生率を抑えることができ、コストダウンを図
ることができた。Therefore, according to the above method, it is possible to simplify the operation of creating artificial seeds, and it is also possible to reduce the incidence of defective products in the process of manipulating artificial seeds compared to the conventional method, thereby reducing costs. .
以下、本発明に係る人工種子の作成方法の実施例をイネ
を例にとり説明する。Hereinafter, an embodiment of the method for producing artificial seeds according to the present invention will be described using rice as an example.
第１図に示すように、まず、人工種子の対象作物の一例
としてのイネにおける種々の植物器官（例えば根、シュ
ート、葉）や、特定の細胞型（例えば内胚乳、花粉）を
Ｎ６培地で脱分化してカルス誘導を行い、そのＮ６培地
で引き続き培養・増殖を行い多数個の未分化な分裂組織
（１）を調整する。この場合、種子の内胚乳、又は、イ
ネ植物体の成長点がカルス誘導の原料としてより好まし
い。As shown in Figure 1, first, various plant organs (e.g. roots, shoots, leaves) and specific cell types (e.g. endosperm, pollen) of rice, which is an example of the target crop for artificial seeds, were grown in N6 medium. Callus is induced by dedifferentiation, followed by culture and proliferation in the N6 medium to prepare a large number of undifferentiated meristems (1). In this case, the endosperm of the seed or the growing point of the rice plant is more preferable as the raw material for callus induction.
次に人工種子化工程について説明する。Next, the artificial seed production process will be explained.
上記分裂組織（１）を（Ｎ６培地＋ＮＡＡ（１ｎａｐｈ
ｔｈａｌｅｎｅ ａｃｅｔｉｃ ａｃｉｄ）０．０
１ｍｇ＃２ ＋ＢＡ（６ｂｅｎｚｙｌａｄｅｎｉｎｅ
）０．１ｍｇ ＋ ４％ショｔＰ’＋１％アルギン酸ナ
トリウム）なる組成の混合液（３）に−様に分散させる
。そして、第２図に示すように、この混合液（３）を５
０ｍＭ塩化カルシウム溶液（１０）中に滴下し、未分化
な分裂組織（１）をゲル状のアルギン酸カルシウムでで
きたカプセル（２）に包み込む。そして、このカプセル
（２）の中には未分化な組織を分化誘導させる作用のあ
るＮＡＡ及びＢＡが含まれているため、前記分裂ｉＪｌ
織（１）がカプセル（２）内で再分化されて幼植物体と
なり、つまり、前記カプセル（２）が人工種子となる。The above meristem (1) (N6 medium + NAA (1naph)
thalene acetic acid) 0.0
1mg #2 + BA (6benzyladenine
) 0.1 mg + 4% ShotP' + 1% sodium alginate). Then, as shown in Figure 2, this mixed solution (3) was
It is dropped into a 0 mM calcium chloride solution (10), and the undifferentiated meristem (1) is encapsulated in a capsule (2) made of gel-like calcium alginate. Since this capsule (2) contains NAA and BA, which have the effect of inducing differentiation of undifferentiated tissue, the division iJl
The weave (1) is redifferentiated within the capsule (2) to become a seedling, that is, the capsule (2) becomes an artificial seed.
なお、カルスに誘導する植物はイネ以外の全ての植物を
対象とすることができる。Note that all plants other than rice can be targeted as plants to be induced into callus.
上記実施例と同様の方法で調整した多数個の未分化な分
裂Ｍ１織（１）を、第３図に示すように、ＮＡＡ及びＢ
Ａ （分化誘導物質）及びショ糖、アミノ酸等の栄養物
を含み、かつ、分裂組織（１）を傷害しない範囲内で加
温し、一定の流動性を持たせたゲル化剤（９）の中に一
様に分散させる。As shown in FIG.
A gelling agent (9) that contains A (differentiation inducing substance) and nutrients such as sucrose and amino acids, is heated within a range that does not damage the meristem tissue (1), and has a certain fluidity. evenly distributed throughout.
そして、このゲル化剤（９）を第１モータ（門、）及び
第１偏心カム（４）で周期的に駆動されるピストン（６
）で一定量ずつノズル（７）から下方の溶液内に押し出
すとともに、溶液内でゲル化した一定量のゲル化剤（９
）を第２モータ（Ｍ２）及び第２偏心カム（５）で駆動
されるカッター（８）で切断してカプセル（２）を形成
してもよい。Then, this gelling agent (9) is applied to a piston (6) which is periodically driven by a first motor (gate) and a first eccentric cam (4).
) into the solution below from the nozzle (7), and a certain amount of the gelling agent (9) gelled in the solution.
) may be cut by a cutter (8) driven by a second motor (M2) and a second eccentric cam (5) to form a capsule (2).
尚、特許請求の範囲の項に図面との対照を便利にする為
に符号を記すが、該記入により本発明は添付図面の構造
に限定されるものではない。Incidentally, although reference numerals are written in the claims section for convenient comparison with the drawings, the present invention is not limited to the structure shown in the accompanying drawings.
図面は本発明に係る人工種子の作成方法の実施例を示し
、第１図はイネから人工種子に導く経路を示す工程図、
第２図は人工種子のカプセル化工程を示す構成図、第３
図は人工種子のカプセル化工程の別実施例を示す構成図
、第４図はイネから人工種子に導く経路の従来例を示す
工程図である。
（１）・・・・・・分裂組織、（２）・・・・・・カプ
セル。The drawings show an example of the method for creating artificial seeds according to the present invention, and FIG. 1 is a process diagram showing the route leading from rice to artificial seeds;
Figure 2 is a block diagram showing the encapsulation process of artificial seeds, Figure 3
The figure is a block diagram showing another embodiment of the encapsulation process for artificial seeds, and FIG. 4 is a process diagram showing a conventional example of the route leading from rice to artificial seeds. (1)... meristematic tissue, (2)... capsule.
Claims (1)
織（１）が増殖可能なカプセル（２）で包み、その後前
記カプセル（２）内で分化誘導することを特徴とする人
工種子の作成方法。An artificial seed characterized by proliferating an undifferentiated meristem (1), wrapping it in a capsule (2) in which the meristem (1) can proliferate, and then inducing differentiation within the capsule (2). How to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26453989A JPH03127920A (en) | 1989-10-11 | 1989-10-11 | Preparation of artificial seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26453989A JPH03127920A (en) | 1989-10-11 | 1989-10-11 | Preparation of artificial seed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03127920A true JPH03127920A (en) | 1991-05-31 |
Family
ID=17404673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP26453989A Pending JPH03127920A (en) | 1989-10-11 | 1989-10-11 | Preparation of artificial seed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03127920A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5660630A (en) * | 1994-03-31 | 1997-08-26 | Yazaki Corporation | Seedlike substance making apparatus |
| US5902398A (en) * | 1995-10-03 | 1999-05-11 | Yazaki Corporation | Artificial seed manufacturing apparatus |
| CN1060909C (en) * | 1996-03-15 | 2001-01-24 | 中国科学院化学研究所 | Artificial seed and preparation method thereof |
| CN1063012C (en) * | 1996-03-15 | 2001-03-14 | 中国科学院化学研究所 | Artificial seed |
| JP2007189999A (en) * | 2006-01-23 | 2007-08-02 | Agritecno Yazaki Co Ltd | Gel-covered seed |
| WO2011085446A1 (en) * | 2010-01-13 | 2011-07-21 | Bses Limited | Methods of plant regeneration and apparatus therefor |
-
1989
- 1989-10-11 JP JP26453989A patent/JPH03127920A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5660630A (en) * | 1994-03-31 | 1997-08-26 | Yazaki Corporation | Seedlike substance making apparatus |
| US5902398A (en) * | 1995-10-03 | 1999-05-11 | Yazaki Corporation | Artificial seed manufacturing apparatus |
| CN1060909C (en) * | 1996-03-15 | 2001-01-24 | 中国科学院化学研究所 | Artificial seed and preparation method thereof |
| CN1063012C (en) * | 1996-03-15 | 2001-03-14 | 中国科学院化学研究所 | Artificial seed |
| JP2007189999A (en) * | 2006-01-23 | 2007-08-02 | Agritecno Yazaki Co Ltd | Gel-covered seed |
| WO2011085446A1 (en) * | 2010-01-13 | 2011-07-21 | Bses Limited | Methods of plant regeneration and apparatus therefor |
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