CN106282296A - A kind of method studying sewage disposal based on simulating riverway - Google Patents
A kind of method studying sewage disposal based on simulating riverway Download PDFInfo
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- CN106282296A CN106282296A CN201610825667.4A CN201610825667A CN106282296A CN 106282296 A CN106282296 A CN 106282296A CN 201610825667 A CN201610825667 A CN 201610825667A CN 106282296 A CN106282296 A CN 106282296A
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- sewage
- thalline
- sewage disposal
- yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
Abstract
The present invention relates to field of environment engineering, a kind of method studying sewage disposal based on simulating riverway, specifically comprise the following steps that actication of culture: the yeast, the bacillus subtilis that are preserved on 4 DEG C of inclined-planes activate, it is lined respectively on corresponding solid medium, cultivate 23 days respectively at 30 DEG C, cultivate 1 day for 37 DEG C；Prepared by seed liquor: the bacterium colony of picking activation, is inoculated in respectively in respective fluid medium, yeast and bacillus subtilis in 28 38 DEG C, 180 200rpm cultivate 12 days；Seed liquor 5000rpm cultivating each bacterial strain to logarithmic (log) phase is centrifuged 5 15min, and the thalline of gained cleans by empty culture medium；The complex situation that energy concentrated expression microorganism of the present invention is faced in river, after the problem that the dissolved oxygen solved in sewage and later stage thalline reduce, the degradation rate of ammonia nitrogen can reach more than 70%, reaches the secondary discharge standard of sewage.
Description
Technical field
The present invention relates to field of environment engineering, a kind of method studying sewage disposal based on simulating riverway.
Technical background
The problem of water environment pollution is difficult to administer at present, along with the development of science and technology, with the processing method such as physics, chemistry
Difference, microorganism can be decomposed Organic substance abundant in sewage by approach such as oxidoreduction fermentations, and be utilized its flourish；Raw
Thing technology the most gradually comes into one's own, and is used widely in fields such as sewage disposals.
Eutrophication problem refers to that the nutrient substance such as nitrogen, phosphorus enters slow flow water bodies, and aquatic ecosystem balance is destroyed
A kind of water body effect, the equipment that currently this type of research is conventional is shaking table, reaction tank and film processing equipment etc., and these are the most unsuitable
It is applied to the truth of river regulation.
Summary of the invention
In order to overcome above-mentioned technical problem, the present invention provides a kind of side studying sewage disposal based on simulating riverway
Method, simulates sewage situation in river course, the ability of microorganism treating sewage is carried out comprehensive study, probes into microorganism in river course
In situation and problem encountered, and for these problems propose solution.
The technical solution adopted for the present invention to solve the technical problems is: one is studied at sewage based on simulating riverway
The method of reason, specifically comprises the following steps that
(1) actication of culture: the yeast, the bacillus subtilis that are preserved on 4 DEG C of inclined-planes activate, and it lines corresponding solid respectively
In culture medium, cultivate 2-3 days respectively at 30 DEG C, cultivate 1 day for 37 DEG C；
(2) prepared by seed liquor: the bacterium colony of picking activation, is inoculated in respectively in respective fluid medium, yeast and hay spore
Bacillus in 28-38 DEG C, 180-200rpm cultivate 1-2 days；
(3) seed liquor 5000rpm cultivating each bacterial strain to logarithmic (log) phase is centrifuged 5-15min, the thalline of gained sky culture medium
Clean, join in sewage, by the sewage adding apparatus of mix homogeneously, start motor, make sewage slowly circulate；And etc.
Away from putting into 2-8 air pump, increase dissolved oxygen in water, beneficially thalline and preferably grow；
(4) monitoring its index ammonia nitrogen, total phosphorus and the change of COD, the cycle is 7-10 days, and adds thalline and nutrition at middle and late stage
Salt.
Described thalline ratio is that bacillus subtilis is than yeast 1-5:1-4.
During in described sewage, the addition of thalline is described sewage, the addition of thalline is that 1L sewage adds 100-300ml bacterium
Body.
Described motor is fully loaded with for 18L, and charging ratio is 70%.
With the cycle for 7 to 10 days, start the monitoring of index.Monitoring according to index finds, when the 3rd to 5 day, index declines
Slowly so that ging up；Same procedure need to be used to cultivate thalline, added to sewage, make the index in sewage drop as far as possible
Low, to reach by the purpose of the contaminated water body of microorganism remediation
Described microzyme culture medium is YEPD culture medium, the most each concentration of component is peptone 1-4g/L, yeast extract 1-3g/L, Portugal
Grape sugar 1-4g/L, PH6.0-7.0,121 DEG C sterilizing 20min.
Described bacillus subtilis bacterium culture medium is broth bouillon, and the most each concentration of component is peptone 1-5g/L, beef
Cream 0.5-2g/L, NaCl 0.5-2g/L, PH7.2-7.4,121 DEG C sterilizing 20min.
The invention has the beneficial effects as follows, a kind of method studying sewage disposal based on simulating riverway, can concentrated expression
The complex situation that microorganism is faced in river, the dissolved oxygen in solving sewage and the problem of later stage thalline minimizing
After, the degradation rate of ammonia nitrogen can reach more than 70%, reaches the secondary discharge standard of sewage, and the degradation rate of total phosphorus reaches more than 30%,
COD degradation rate can reach more than 60%.
Detailed description of the invention
Embodiment 1
The every Raw performance of sewage is ammonia nitrogen 173.4mg/L, total phosphorus be 63.2mg/L, COD be 775mg/L；By bacillus subtilis
Bacterium is that the seed liquor after 2:4 cultivates is centrifuged than yeast, takes thalline, adds in simulating riverway sewage device；By air mass flow it is
In the equidistant embedding device of air pump of 1.8L/min, group one puts into 3, and group two puts into 5, and group two puts into 7；Other condition phases
With, index is monitored.
Final ammonia nitrogen degradation rate is respectively 63.3%, 75.6%, 77.1%, the degradation rate of total phosphorus is respectively 22.3%, 30.0%,
30.2%, COD degradation rate is respectively 45.5%, 50.8%, 53.4%；Embodiment 1 shows, during group one only 3 air pumps, molten in sewage
Oxygen not, and organize two and the individual degradation rate difference of group three inconspicuous, it was demonstrated that preferably use 5 air pumps.
Embodiment 2
The every Raw performance of sewage is ammonia nitrogen 179.7mg/L, total phosphorus be 71.4mg/L, COD be 855mg/L；Organize adding of a thalline
Add ratio be bacillus subtilis be 1:1 than yeast, organizing two thalline bacillus subtilises is 5:3 than yeast, and group three is withered
Grass bacillus cereus is 2:4 than yeast；Equidistantly adding 5, the air pump of air mass flow 1.8L/min, other conditions are identical,
Index is monitored.
Final ammonia nitrogen degradation rate is respectively 72.6%, 63.8%, 78.4%, the degradation rate of total phosphorus is respectively 29.7%, 34.2%,
The degradation rate of 30.8%, COD is respectively 53.7%, 58.3%, 51.5%；Embodiment 2 shows, bacillus subtilis is more conducive to ammonia of degrading
Nitrogen, yeast is more conducive to degradation of ammonia nitrogen, can adjust the ratio between thalline according to the Raw performance of handled sewage, make thalline utilize
Rate reaches optimal.
Claims (6)
1. the method studying sewage disposal based on simulating riverway, it is characterised in that specifically comprise the following steps that
(1) actication of culture: the yeast, the bacillus subtilis that are preserved on 4 DEG C of inclined-planes activate, and it lines corresponding solid respectively
In culture medium, cultivate 2-3 days respectively at 30 DEG C, cultivate 1 day for 37 DEG C；
(2) prepared by seed liquor: the bacterium colony of picking activation, is inoculated in respectively in respective fluid medium, yeast and hay spore
Bacillus in 28-38 DEG C, 180-200rpm cultivate 1-2 days；
(3) seed liquor 5000rpm cultivating each bacterial strain to logarithmic (log) phase is centrifuged 5-15min, the thalline of gained sky culture medium
Clean, join in sewage, by the sewage adding apparatus of mix homogeneously, start motor, make sewage slowly circulate；And equidistantly put
Enter 2-8 air pump；
(4) monitoring its index ammonia nitrogen, total phosphorus and the change of COD, the cycle is 7-10 days, and adds thalline and nutrition at middle and late stage
Salt.
The method studying sewage disposal based on simulating riverway the most according to claim 1, it is characterised in that described bacterium
Body ratio is that bacillus subtilis is than yeast 1-5:1-4.
The method studying sewage disposal based on simulating riverway the most according to claim 1, it is characterised in that described dirt
In water, the addition of thalline is that 1L sewage adds 100-300ml thalline.
The method studying sewage disposal based on simulating riverway the most according to claim 1, it is characterised in that described electricity
Machine is fully loaded with for 18L, and charging ratio is 70%.
The method studying sewage disposal based on simulating riverway the most according to claim 1, it is characterised in that described ferment
Female bacterium culture medium is YEPD culture medium, and the most each concentration of component is peptone 1-4g/L, yeast extract 1-3g/L, glucose 1-4g/
L, PH6.0-7.0,121 DEG C of sterilizing 20min.
The method studying sewage disposal based on simulating riverway the most according to claim 1, it is characterised in that described
Bacillus subtilis bacterium culture medium is broth bouillon, the most each concentration of component is peptone 1-5g/L, Carnis Bovis seu Bubali cream 0.5-2g/L,
NaCl 0.5-2g/L, PH7.2-7.4,121 DEG C of sterilizing 20min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610825667.4A CN106282296A (en) | 2016-09-18 | 2016-09-18 | A kind of method studying sewage disposal based on simulating riverway |
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| CN201610825667.4A CN106282296A (en) | 2016-09-18 | 2016-09-18 | A kind of method studying sewage disposal based on simulating riverway |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106987536A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | Complex micro organism fungicide for river regulation and its preparation method and application |
| CN106987535A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | It is a kind of for complex micro organism fungicide of river regulation and preparation method thereof, river sewage processing method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101898825A (en) * | 2010-07-16 | 2010-12-01 | 浙江大学 | Simulating riverway biological respinse unit for repairing light polluted water body |
| CN202039320U (en) * | 2011-04-18 | 2011-11-16 | 中国科学院水生生物研究所 | Experimental system device for simulating natural river course |
| CN103966126A (en) * | 2014-04-25 | 2014-08-06 | 北京山地生态科技研究所 | Bacillus subtilis, microbial agent and application of bacillus subtilis and microbial agent in ecological restoration |
| CN104178433A (en) * | 2014-09-04 | 2014-12-03 | 山东省农业科学院生物技术研究中心 | Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation |
| CN104911120A (en) * | 2015-03-11 | 2015-09-16 | 浙江理工大学 | Bacillus subtilis fermentation production technology |
| CN105585129A (en) * | 2016-01-12 | 2016-05-18 | 南京大学 | Device and method for simulating fate of nitrogen in in-situ river channel ecosystem |
-
2016
- 2016-09-18 CN CN201610825667.4A patent/CN106282296A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101898825A (en) * | 2010-07-16 | 2010-12-01 | 浙江大学 | Simulating riverway biological respinse unit for repairing light polluted water body |
| CN202039320U (en) * | 2011-04-18 | 2011-11-16 | 中国科学院水生生物研究所 | Experimental system device for simulating natural river course |
| CN103966126A (en) * | 2014-04-25 | 2014-08-06 | 北京山地生态科技研究所 | Bacillus subtilis, microbial agent and application of bacillus subtilis and microbial agent in ecological restoration |
| CN104178433A (en) * | 2014-09-04 | 2014-12-03 | 山东省农业科学院生物技术研究中心 | Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation |
| CN104911120A (en) * | 2015-03-11 | 2015-09-16 | 浙江理工大学 | Bacillus subtilis fermentation production technology |
| CN105585129A (en) * | 2016-01-12 | 2016-05-18 | 南京大学 | Device and method for simulating fate of nitrogen in in-situ river channel ecosystem |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106987536A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | Complex micro organism fungicide for river regulation and its preparation method and application |
| CN106987535A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | It is a kind of for complex micro organism fungicide of river regulation and preparation method thereof, river sewage processing method |
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