CN105293689A - Culturing method for phenol-tolerating anaerobic-ammonia-oxidation-granule-sludge culture - Google Patents
Culturing method for phenol-tolerating anaerobic-ammonia-oxidation-granule-sludge culture Download PDFInfo
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Abstract
The invention provides a culturing method for a phenol-tolerating anaerobic-ammonia-oxidation-granule-sludge culture. The culturing method includes the steps that an upflowing anaerobic-sludge-bed reactor is adopted, anaerobic-ammonia-oxidation granule sludge serves as an inoculating source, simulated wastewater with ammonia nitrogen and nitrite nitrogen serves as fed water, and culturing is carried out under the conditions that the anaerobic and photophobic conditions are achieved, temperature is 35+/-1 DEG C, the pH of the fed water is 8.10+/-0.14, the hydraulic retention time is 0.8 h to 2.0 h; the granule sludge is cultured through simulated wastewater without phenol for 2 weeks to 5 weeks till the reactor stably runs, then the phenol is added into the fed water in the mode that the concentration is gradually reduced in the early stage and rapidly increased in the later stage, culturing is carried out for 2 weeks to 12 weeks under the each concentration condition, and culturing is completed when the microbial activity in the reactor is 4-5 times that of the anaerobic-ammonia-oxidation granule sludge obtained after the phenol is added for the first time. The anaerobic-ammonia-oxidation granule sludge cultured with the method has the good phenol tolerating capacity, and is excellent in character and high in activity.
Description
(1) technical field
The present invention relates to a kind of cultural method of anaerobic ammonium oxidation granular sludge culture, particularly a kind of cultural method tolerating phenol anaerobic ammonium oxidation granular sludge culture.
(2) background technology
Anammox, as a kind of Process of Biological Nitrogen Removal, has broad application prospects in wastewater biological denitrificaion field with efficient advantage.And what bear this technique core roles is anaerobic ammonia oxidizing bacteria.
At present, the research of anaerobic ammonia oxidation process in wastewater treatment mostly concentrate on simulation inorganic wastewater or high ammonia nitrogen, low ratio of carbon to ammonium actual waste water.But all containing the organism that quantity differs in nearly all actual waste water.Anammox Pseudomonas autotrophic microorganism, with CO
2for sole carbon source, it is generally acknowledged that organism has restraining effect to it.Phenol is as important Organic Chemicals, be present in as in many trade effluents such as pulp mill, textile industry, petroleum chemical plant and coking plant, it is one of common causing toxicity organism of four classes, can suppress by poisoning microorganism or making corresponding biological enzyme inactivation produce anaerobic ammonia oxidizing bacteria, and this suppression is irreversible often.Therefore, use based on the anaerobic ammonia oxidation process of Anammox culture, denitrogenation processing is carried out to these waste water time, likely can face the risk of anaerobic ammonia oxidizing bacteria inactivation, and then affect the operation stability for the treatment of system.
Given this, the anaerobic ammonium oxidation granular sludge cultivating tolerance phenol is of great advantage containing the trade effluent of phenol for process.Although existing research uses Treatment of Coking Effluent factory mud success enrichment anaerobic ammonium oxidation granular sludge, and the anaerobic ammonium oxidation granular sludge of tolerance phenol is cultivated by progressively improving phenol content in coking simulated wastewater, but this training method is lasted longer, in actual waste water process, there is no clear superiority.Research finds by progressively to reduce early stage and the later stage improves rapidly the mode of phenol concentration, anaerobic ammonium oxidation granular sludge can be made to experience adaptive phase and domestication thus possess the ability of tolerance phenol fast, and sludge activity is better.
(3) summary of the invention
The object of the invention is the cultural method of the anaerobic ammonium oxidation granular sludge culture for obtaining tolerance phenol, by progressively reduction and later stage improve rapidly the mode of phenol concentration in earlier stage, anaerobic ammonium oxidation granular sludge can be made to experience adaptive phase and domestication thus possess the ability of tolerance phenol fast, and sludge activity is better, for anaerobic ammonia oxidation process creates approach in the application of the wastewater treatments such as the trade effluent, particularly coking chemical waste water containing phenolic compound.
The technical solution used in the present invention is:
The invention provides a kind of cultural method tolerating phenol anaerobic ammonium oxidation granular sludge culture, described method is: adopt upflow anaerobic sludge blanket reactor, take anaerobic ammonium oxidation granular sludge as inoculation source, microorganism active is detected before initial launch, with the simulated wastewater containing ammonia nitrogen and nitrite nitrogen for water inlet, in anaerobism, lucifuge, temperature is 35 ± 1 DEG C, inlet flow-patterm is 8.10 ± 0.14, hydraulic detention time be under the condition of 0.8 ~ 2.0h cultivate 2 ~ 5 thoughtful reactors stable after, first in water inlet, add phenol progressively to reduce concentration style, continuing to be cultured to microorganism active in reactor is initial launch anaerobic ammonium oxidation granular sludge bioactive 65% ~ 75% when stablizing, then in water inlet, phenol is added in the mode improving rapidly concentration, continue to be cultured to reactor endoparticle sludge activity reach reactor stable after add phenol first after active 4 ~ 5 times, namely obtain the anaerobic ammonium oxidation granular sludge culture of tolerance phenol, described simulated wastewater consists of: ammonia nitrogen 70 ~ 280mgL
-1, nitrite nitrogen 70 ~ 280mgL
-1, KH
2pO
45 ~ 20mgL
-1, CaCl
22H
2o1 ~ 10mgL
-1, MgSO
42H
2o200 ~ 400mgL
-1, KHCO
3800 ~ 1500mgL
-1, solvent is water, the each add-on of described phenol with water inlet in phenol quality final concentration for 12.5 ~ 50mgL
-1, described anaerobic ammonium oxidation granular sludge in the biomass of volatile solid concentration VSS for 12 ~ 30gL
-1.
Further, the mode that described phenol adopts in earlier stage progressively reduction and later stage to improve rapidly concentration adds, and add-on is with the quality final concentration of phenol in water inlet, and the reduction amplitude progressively reducing phenol concentration described early stage is 50%; It is point in water inlet that to add the quality final concentration adding phenol after phenol makes the quality final concentration of phenol in water stable with reactor first identical that the described later stage improves rapidly phenol concentration.
Further, described phenol Adding Way is: in water inlet, add phenol first early stage and make its quality final concentration be 50mgL
-1, cultivate 3 weeks for 35 ± 1 DEG C, second time adds phenol in water inlet makes its quality final concentration be 25mgL
-1, cultivate 2 weeks for 35 ± 1 DEG C, progressively reducing phenol concentration in intaking, to continue to be cultured to microorganism active in reactor be initial launch anaerobic ammonium oxidation granular sludge bioactive 65% ~ 75% when stablizing; Then in water inlet, adding phenol makes its quality final concentration be 50mgL
-1, 35 ± 1 DEG C continue microorganism active in cultivation 3 thoughtful reactors be reactor stable after add bioactive 4 ~ 5 times of anaerobic ammonium oxidation granular sludge after phenol first.
Further, described simulated wastewater is composed as follows: ammonia nitrogen 70 ~ 280mgL
-1, nitrite nitrogen 70 ~ 280mgL
-1, KH
2pO
410mgL
-1, CaCl
22H
2o7.4mgL
-1, MgSO
42H
2o300mgL
-1, KHCO
31250mgL
-1, solvent is water.
Further, the inoculation volume of described anaerobic ammonium oxidation granular sludge accounts for 30% ~ 90% of reactor cumulative volume.
Further, in described water inlet, the ratio of the amount of substance of ammonia nitrogen and nitrite nitrogen is 1:1.
The cultural method of tolerance phenol anaerobic ammonium oxidation granular sludge of the present invention carries out as follows: adopt upflow anaerobic sludge blanket reactor, at 35 ± 1 DEG C, inoculation accounts for the anaerobic ammonium oxidation granular sludge of 30% ~ 90% of reactor cumulative volume, to contain 266mgL
-1ammonia nitrogen and 266mgL
-1the simulated wastewater of nitrite nitrogen is water inlet, and adopting Continuous Flow, is 0.8 ~ 2.0h at hydraulic detention time, anaerobism, lucifuge, and inlet flow-patterm is that under the condition of 8.10 ± 0.14, cultivation 2 ~ 5 thoughtful reactors are stable; Early stage progressively reduces the phenol concentration stage, adds phenol first and make its quality final concentration be 50mgL in water inlet
-1, cultivate 3 weeks at 35 ± 1 DEG C, second time adds phenol in water inlet makes its quality final concentration be 25mgL
-1, cultivate 2 weeks at 35 ± 1 DEG C, the amplitude with 50% progressively reduces phenol concentration in water inlet, and to continue to be cultured to microorganism active in reactor be anaerobic ammonium oxidation granular sludge when initial launch is stablized bioactive 65% ~ 75%; Then in water inlet, adding phenol makes its quality final concentration be 50mgL
-1, cultivate 3 ~ 4 weeks at 35 ± 1 DEG C, to reactor endoparticle sludge activity reach reactor stable after add 4 ~ 5 times active after phenol first, obtain the anaerobic ammonium oxidation granular sludge culture tolerating phenol; The biomass of described inoculation anaerobic ammonium oxidation granular sludge is 16.7gL
-1, activity is 245mgNg
-1vSSd
-1.
The present invention is by progressively reduction and later stage improve rapidly the mode of phenol concentration in water inlet in earlier stage, anaerobic ammonium oxidation granular sludge can be made to experience adaptive phase and domestication thus possess the ability of tolerance phenol fast, and sludge activity is better, test proves that this method is effective for cultivating the anaerobic ammonium oxidation granular sludge tolerating phenol.
Beneficial effect of the present invention is mainly reflected in: the granule sludge that 1. the present invention cultivates can tolerate 50mgL
-1phenol, has excavated the practical application potential of anaerobic ammonia oxidation process; 2. significantly shorten domestication culture cycle.
(4) accompanying drawing explanation
Fig. 1 is the structural representation of upflow anaerobic sludge blanket reactor; In Fig. 1: 1. lower cone; 2. water-in; 3. reactor body; 4. go up frustum; 5. settling region; 6. water outlet; 7. triphase separator.
(5) embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in this:
Embodiment 1:
In Continuous Flow, adopt upflow anaerobic sludge blanket reactor (Fig. 1), at 35 ± 1 DEG C, the sludge organism amount represented with volatile suspended solid concentration VSS is 16.7gL
-1, granule sludge activity is 245mgNg
-1vSSd
-1anaerobic ammonium oxidation granular sludge be inoculation source, with containing 266mgL
-1ammonia nitrogen and 266mgL
-1the simulated wastewater of nitrite nitrogen is water inlet, and hydraulic detention time is 0.8h, and in anaerobism, lucifuge, inlet flow-patterm is cultivate mud under the condition of 8.10 ± 0.14.Wherein in simulated wastewater used, other component is composed as follows: KH
2pO
410mgL
-1, CaCl
22H
2o7.4mgL
-1, MgSO
42H
2o300mgL
-1, KHCO
31250mgL
-1, solvent is water.Upflow anaerobic sludge blanket reactor (as Fig. 1) that two effective volumes are 1.0L is set as a control group and test group.Control group is one group that is left intact, and test group is in water inlet, add finite concentration phenol on this basis one group.Reactor is directly placed in the thermostatic chamber of 35 ± 1 DEG C, through within 2 ~ 5 weeks, not cultivate containing the simulated wastewater of phenol stable after, control group granule sludge activity is 342mgNg
-1vSSd
-1, the activity of test group granule sludge is 271.2mgNg
-1vSSd
-1, enter subsequently early stage progressively reduce the phenol concentration stage, test group first phenol addition with water inlet in quality final concentration for 50mgL
-1, cultivate 3 weeks, now the activity of control group granule sludge is 156mgNg
-1vSSd
-1, the activity of test group granule sludge is 14.4mgNg
-1vSSd
-1; In second time water inlet, phenol quality final concentration is 25mgL
-1, after cultivating 2 weeks, the activity of control group granule sludge is 213.6mgNg
-1vSSd
-1, the activity of test group granule sludge is 31.2mgNg
-1vSSd
-1; In last water inlet, phenol quality final concentration is 12.5mgL
-1, after cultivating 5 weeks, the activity of control group granule sludge is 213.6mgNg
-1vSSd
-1, the activity of test group granule sludge is 24mgNg
-1vSSd
-1, continue cultivation after 7 weeks, the activity of control group granule sludge is 288mgNg
-1vSSd
-1, the activity of test group granule sludge is 192mgNg
-1vSSd
-1, compared with first 5 weeks, granule sludge enters the adaptive phase, and test group activity improves 8 times; And then entering the later stage improves concentration period rapidly, now in water inlet, adds phenol and makes its quality final concentration be increased to 50mgL
-1, after cultivating 3 weeks, the activity of control group granule sludge is 393.6mgNg
-1vSSd
-1, the activity of test group granule sludge is 70mgNg
-1vSSd
-1, go through identical incubation time, the test group granule sludge activity through domestication is approximately 4.7 times that add granule sludge activity after same concentrations phenol first, and the sustainable steady running of reactor, obtain tolerance phenol anaerobic ammonium oxidation granular sludge culture.Thus, anaerobic ammonium oxidation granular sludge is progressively reducing adaptive phase of phenol concentration through early stage, and after the later stage improves rapidly the domestication of phenol concentration, can obtain the tolerance of Pyrogentisinic Acid, proterties is excellent, and better active.
Claims (7)
1. one kind tolerates the cultural method of phenol anaerobic ammonium oxidation granular sludge culture, it is characterized in that described method is: adopt upflow anaerobic sludge blanket reactor, take anaerobic ammonium oxidation granular sludge as inoculation source, with the simulated wastewater containing ammonia nitrogen and nitrite nitrogen for water inlet, in anaerobism, lucifuge, temperature is 35 ± 1 DEG C, inlet flow-patterm is 8.10 ± 0.14, hydraulic detention time be under the condition of 0.8 ~ 2.0h cultivate 2 ~ 5 thoughtful reactors stable after, first in water inlet, add phenol progressively to reduce concentration style, continuing to be cultured to microorganism active in reactor is initial launch anaerobic ammonium oxidation granular sludge bioactive 65% ~ 75% when stablizing, then in water inlet, add phenol in the mode improving rapidly concentration, continue to be cultured to reactor endoparticle sludge activity reach add phenol first after active 4 ~ 5 times, namely obtain the anaerobic ammonium oxidation granular sludge culture of tolerance phenol, described simulated wastewater consists of: ammonia nitrogen 70 ~ 280mgL
-1, nitrite nitrogen 70 ~ 280mgL
-1, KH
2pO
45 ~ 20mgL
-1, CaCl
22H
2o1 ~ 10mgL
-1, MgSO
42H
2o200 ~ 400mgL
-1, KHCO
3800 ~ 1500mgL
-1, solvent is water, the each add-on of described phenol with the quality final concentration of phenol in water inlet for 12.5 ~ 50mgL
-1, described anaerobic ammonium oxidation granular sludge biomass is 12 ~ 30gL
-1.
2. the method for claim 1, it is characterized in that: the mode that described phenol adopts in earlier stage progressively reduction and later stage to improve rapidly phenol concentration adds, add-on is with the quality final concentration of phenol in water inlet, and when described early stage progressively reduces, in water inlet, the reduction amplitude of phenol concentration is 50%; It is point in water inlet that to add the quality final concentration adding phenol after phenol makes the quality final concentration of phenol in water stable with reactor first identical that the described later stage improves rapidly phenol concentration.
3. method as claimed in claim 2, is characterized in that described phenol Adding Way is: in water inlet, add phenol first early stage and make its quality final concentration be 50mgL
-1, cultivate 3 weeks for 35 ± 1 DEG C, then add phenol and make its quality final concentration be 25mgL in intaking
-1, cultivate 2 weeks for 35 ± 1 DEG C, progressively reducing phenol concentration in intaking, to continue to be cultured to microorganism active in reactor be initial launch anaerobic ammonium oxidation granular sludge bioactive 65% ~ 75% when stablizing; Then in water inlet, adding phenol makes its quality final concentration be 50mgL
-1, continue microorganism active in cultivation 3 thoughtful reactor be reactor stable after add bioactive 4 ~ 5 times of anaerobic ammonium oxidation granular sludge after phenol first.
4. the method for claim 1, is characterized in that, described simulated wastewater is composed as follows: ammonia nitrogen 70 ~ 280mgL
-1, nitrite nitrogen 70 ~ 280mgL
-1, KH
2pO
410mgL
-1, CaCl
22H
2o7.4mgL
-1, MgSO
42H
2o300mgL
-1, KHCO
31250mgL
-1, solvent is water.
5. method as claimed in claim 1, it is characterized in that, the inoculation volume of described anaerobic ammonium oxidation granular sludge accounts for 30% ~ 90% of reactor cumulative volume.
6. method as claimed in claim 1, it is characterized in that, in described water inlet, the ratio of the amount of substance of ammonia nitrogen and nitrite nitrogen is 1:1.
7. method as claimed in claim 1, is characterized in that described method is carried out as follows: adopt upflow anaerobic sludge blanket reactor, and at 35 ± 1 DEG C, inoculation accounts for the anaerobic ammonium oxidation granular sludge of 30% ~ 90% of reactor cumulative volume, with containing 266mgL
-1ammonia nitrogen and 266mgL
-1the simulated wastewater of nitrite nitrogen is water inlet, and adopt Continuous Flow, hydraulic detention time is 0.8 ~ 2.0h, and in anaerobism, lucifuge, inlet flow-patterm is that to be cultured to reactor under the condition of 8.10 ± 0.14 stable; In water inlet, adding phenol first makes its final concentration be 50mgL
-1, cultivate 3 weeks at 35 ± 1 DEG C, second time adds phenol in water inlet makes its quality final concentration be 25mgL
-1, cultivate 2 weeks at 35 ± 1 DEG C, the amplitude with 50% progressively reduces phenol concentration in water inlet, and to continue to be cultured to microorganism active in reactor be anaerobic ammonium oxidation granular sludge when initial launch is stablized bioactive 65% ~ 75%; Then in water inlet, adding phenol makes its quality final concentration be 50mgL
-1, cultivate 3 ~ 4 weeks at 35 ± 1 DEG C, to reactor endoparticle sludge activity reach reactor stable after add 4 ~ 5 times active after phenol first, obtain the anaerobic ammonium oxidation granular sludge culture tolerating phenol; The biomass of described inoculation anaerobic ammonium oxidation granular sludge is 16.7gL
-1, activity is 245mgNg
-1vSSd
-1.
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Cited By (3)
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CN112047464A (en) * | 2020-08-04 | 2020-12-08 | 北京工业大学 | Culture method of tolerant low-dose fulvic acid anaerobic ammonium oxidation granular sludge |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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