CN111875049A - Strain domestication method for improving activity of anaerobic granular sludge and application - Google Patents
Strain domestication method for improving activity of anaerobic granular sludge and application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2833—Anaerobic digestion processes using fluidized bed reactors
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The invention relates to a strain domestication method for improving anaerobic granular sludge activity and application thereof, wherein the domestication method comprises the following steps: 1) preparing simulated wastewater; 2) adding the volcanic rock filler into an EGSB anaerobic reactor; 3) inoculating hydrolysis acidification facultative anaerobic activated sludge, and externally circulating simulated wastewater which does not enter a water tank for 2-3 days through a circulating pump; 4) the EGSB anaerobic reactor starts to continuously feed the simulated wastewater in the water distribution tank; 5) adding benzene or ether toxic substances into the entered simulated wastewater, completing the domestication of the strains for improving the activity of the anaerobic granular sludge, and obtaining the volcanic rock filler attached with the strains for improving the activity of the anaerobic granular sludge. The volcanic rock filler attached with the strain is added into the EGSB anaerobic reactor for treating the organic wastewater containing toxic substances, so that the inhibition effect of the toxic substances on the anaerobic granular sludge is effectively avoided, the activity of the anaerobic granular sludge is improved, the COD concentration of effluent is reduced, and the organic treatment load of the EGSB anaerobic reactor is improved.
Description
Technical Field
The invention relates to the technical field of strain domestication and environment-friendly water treatment, in particular to a strain domestication method for improving the activity of anaerobic granular sludge and application thereof.
Background
At present, the anaerobic biotechnology is widely applied to the industries of petroleum, chemical industry, steel, gas generation stations, mechanical processing and the like.
An anaerobic Expanded granular sludge bed reactor (EGSB) is a 3 rd generation high-efficiency anaerobic reactor researched and developed by Lettinga professor of Wageningen university in the Netherlands in the later 80 th century in an upflow anaerobic sludge bed reactor, and has the advantages of low sludge yield, small floor area, strong impact resistance and the like, and particularly has more remarkable treatment effect on high-concentration organic wastewater. The core role of the process is the activity of anaerobic granular sludge.
The activities affecting the anaerobic granular sludge include, besides temperature, pH, nutrients, toxic substances in organic wastewater, and common toxic organic substances include phenol, ether, aldehyde and the like, and the toxic substances inhibit the anaerobic granular sludge by poisoning microorganisms or inactivating corresponding biological enzymes, and the inhibition is irreversible. Therefore, when EGSB is used to treat these wastewaters, the risk of inactivation of anaerobic granular sludge may be encountered, which in turn affects the operational stability of the treatment system.
Disclosure of Invention
The invention aims to solve the problems in the background art and provides a strain acclimatization method and application for improving the activity of anaerobic granular sludge, which are greatly beneficial to an EGSB (expanded granular sludge bed) anaerobic reactor to treat organic wastewater containing toxic substances.
A strain domestication method for improving the activity of anaerobic granular sludge comprises the following steps:
1) preparing simulated wastewater: the simulated wastewater comprises 2000mg/L of carbon source, 20mg/L of urea, 4mg/L of monopotassium phosphate, 50 mu g/L of quicklime, 62.5 mu g/L of magnesium sulfate and 25 mu g/L of trace elements, and the solvent is fresh water.
2) Adding the volcanic rock filler into an EGSB anaerobic reactor.
3) And then putting one part of the simulated wastewater prepared in the step 1) into a water distribution tank, putting the other part into an EGSB anaerobic reactor, inoculating hydrolytic acidification facultative anaerobic activated sludge in the EGSB anaerobic reactor at the temperature of 36.5-39.5 ℃, and externally circulating the simulated wastewater which does not enter the water distribution tank for 2-3 days by a circulating pump.
4) The EGSB anaerobic reactor starts to continuously feed the simulated wastewater in the distribution water tank, and the residual hydrolytic acidification facultative anaerobic activated sludge in the EGSB anaerobic reactor and on the surface of the volcanic rock filler is taken away by means of higher hydraulic power rising flow velocity.
5) Adding benzene or ether toxic substances into the entered simulated wastewater;
finally, the EGSB anaerobic reactor runs stably, the COD of the effluent of the EGSB anaerobic reactor reaches more than 90% of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added, the strain acclimation for improving the activity of the anaerobic granular sludge is completed, and the volcanic rock filler attached with the strain for improving the activity of the anaerobic granular sludge is obtained.
In one embodiment, the carbon source in step 1) is provided by glucose, or sodium acetate, or citric acid, or ethanol.
In one embodiment, in the step 2), the volcanic stone filler is a spherical volcanic stone filler which is obtained through screening and polishing, a plurality of pinholes are formed in the volcanic stone filler, the particle size of the volcanic stone filler is 2-6 mm, and the specific gravity of the volcanic stone filler is 1.01-1.10 g/cm3The hollow rate is 48-60%, and the surface of the volcanic rock filler is rough.
In one embodiment, in the step 2), the adding amount of the volcanic rock filler is 30-60% of the total volume of the EGSB anaerobic reactor.
In one embodiment, the hydrolysis acidification facultative anaerobic activated sludge in the step 3) is obtained from a hydrolysis acidification tank of a municipal or sewage treatment plant, the sludge concentration is 8 g/L-12 g/L, and the sludge sedimentation ratio is 30% -60%.
In the step 3), the inoculation amount of the hydrolytic acidification facultative anaerobic activated sludge accounts for 60-80% of the total volume of the EGSB anaerobic reactor.
In one embodiment, in the step 4), the EGSB anaerobic reactor starts to continuously feed the simulated wastewater in the water distribution tank, the hydraulic retention time is 10-30 h, the ascending flow rate is 4-10 m/h, and the operation is carried out for 2-3 days; meanwhile, residual hydrolytic acidification facultative anaerobic activated sludge in the EGSB anaerobic reactor and on the surface of the volcanic rock filler is taken away by means of higher hydraulic power rising flow velocity, so that dead sludge enrichment is prevented.
In one embodiment, the method for adding benzene or ether toxic substances into the simulated wastewater in the step 5) comprises the following steps:
a) benzene or ether toxic substances are added into the entered simulated wastewater for the first time to ensure that the mass concentration of the benzene or ether toxic substances is 60mg/L, the wastewater is cultured for 3-4 weeks at the temperature of 36.5-39.5 ℃, and the COD of the effluent reaches 65-75 percent of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added.
b) Then benzene or ether toxic substances are added into the entered simulated wastewater to ensure that the mass concentration of the benzene or ether toxic substances is 35mg/L, the wastewater is cultured for 2-3 weeks at the temperature of 36.5-39.5 ℃, and the COD of the effluent reaches 75-85% of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added.
c) Then benzene or ether toxic substances are added into the entered simulated wastewater to ensure that the mass concentration is 15mg/L, the wastewater is cultured for 2 weeks at the temperature of 36.5-39.5 ℃, and the COD of the effluent reaches more than 90 percent of the COD of the effluent of the EGSB anaerobic reactor when the toxic substances are not added.
d) And gradually increasing the mass concentration of the benzene or ether toxic substances in the entered simulated wastewater to 120mg/L in the later period, wherein the adding amount is 1.3-2.5 times of that of the toxic substances in the simulated wastewater each time, and the culture period is 3-5 weeks.
In one embodiment, the ether-based toxic substance in step 5) is diethyl ether.
In one embodiment, the EGSB anaerobic reactor comprises an EGSB body reactor, a sampling port, a feed port, a wet gas flow meter, a reactor outlet, a three-phase separator and a circulating pump.
The water distribution tank is communicated with the bottom in the EGSB main body reactor through a water inlet pump. The side surface of the EGSB main body reactor is sequentially provided with a reactor outlet, a feed inlet and a sampling port from top to bottom. The outlet of the reactor is divided into two paths, one path is connected with the outside, and the other path is communicated with the bottom in the EGSB main reactor through a circulating pump. The three-phase separator is arranged at the top inside the EGSB main body reactor, and the wet gas flowmeter is arranged at the top outside the EGSB main body reactor.
The application of the volcanic rock filler attached with the strain for improving the activity of the anaerobic granular sludge, which is obtained by the strain domestication method for improving the activity of the anaerobic granular sludge, comprises the following steps: adding the volcanic rock filler attached with the strain for improving the activity of the anaerobic granular sludge into the EGSB anaerobic reactor for treating the organic wastewater containing toxic substances, wherein the adding amount is 1-15% of the total volume of the EGSB anaerobic reactor.
The invention has the advantages and beneficial effects that:
the invention utilizes biological filler volcanic rock, namely volcanic rock filler, to obtain strains through culturing and domesticating facultative anaerobic activated sludge in a special water quality environment by hydrolyzing and acidifying facultative anaerobic activated sludge, the strains are attached in needle holes of the volcanic rock filler, the volcanic rock filler attached with the strains is added into an EGSB anaerobic reactor for treating organic wastewater containing toxic substances, the inhibition effect of the toxic substances on the anaerobic granular sludge is effectively avoided, thereby improving the activity of the anaerobic granular sludge, reducing the COD concentration of effluent and improving the organic treatment load of the EGSB anaerobic reactor, and tests prove that the method is effective for domesticating the strains capable of improving the activity of the anaerobic granular sludge.
Drawings
FIG. 1 is a schematic view of the state of an EGSB anaerobic reactor during acclimation.
FIG. 2 is a schematic view showing the state of an EGSB anaerobic reactor in the application process.
Fig. 3 is a schematic structural diagram of a volcanic rock filler.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "disposed" on another element, it can be directly disposed or attached to the other element or intervening elements may also be present. The terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," and the like are used in an orientation or positional relationship indicated in the drawings for ease of description and simplicity of description, but do not indicate or imply that the referenced device or element must have a particular orientation, be constructed in a particular orientation, and be operated.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Example 1
Referring to fig. 1, a strain acclimation method for improving anaerobic granular sludge activity includes the following steps:
1) preparing simulated wastewater: the simulated wastewater comprises 2000mg/L of carbon source, 20mg/L of urea, 4mg/L of monopotassium phosphate, 50 mu g/L of quicklime, 62.5 mu g/L of magnesium sulfate and 25 mu g/L of trace elements, and the solvent is fresh water. The carbon source is provided by glucose, or sodium acetate, or citric acid, or ethanol.
2) Adding the volcanic rock filler 10 into the EGSB anaerobic reactor, wherein the adding amount is 45 percent of the total volume of the EGSB anaerobic reactor.
3) Then, one part of the simulated wastewater prepared in the step 1) is put into a water distribution tank 1, and the other part is put into an EGSB anaerobic reactor; the hydrolysis acidification facultative anaerobic activated sludge 11 is inoculated in the EGSB anaerobic reactor at the temperature of 36.5-39.5 ℃, the inoculation amount accounts for 65 percent of the total volume of the EGSB anaerobic reactor, and the simulated wastewater which is not added into the water distribution tank 1 is externally circulated for 3 days by a circulating pump 9.
The hydrolysis acidification facultative anaerobic activated sludge is obtained from a hydrolysis acidification pool of a sewage treatment plant, the sludge concentration is 10.4526g/L, and the sludge sedimentation ratio is 48%.
4) The EGSB anaerobic reactor starts to continuously feed the simulated wastewater in the water distribution tank 1, the hydraulic retention time is 20h, the ascending flow rate is 7m/h, the operation is carried out for 3 days, the COD of the effluent of the EGSB anaerobic reactor tends to be stable, and the volcanic rock filler film-forming is finished; meanwhile, residual hydrolytic acidification facultative anaerobic activated sludge in the EGSB anaerobic reactor and on the surface of the volcanic rock filler is taken away by means of higher hydraulic power rising flow velocity, so that the enrichment of dead sludge is prevented, and the treatment effect of the EGSB anaerobic reactor is prevented from being influenced.
5) Adding benzene or ether toxic substances into the entered simulated wastewater, comprising the following steps:
a) adding ether into the entered simulated wastewater for the first time to ensure that the mass concentration of the ether is 60mg/L, culturing for 4 weeks at the temperature of 36.5-39.5 ℃, and ensuring that the COD of the effluent reaches 72.4 percent of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added.
b) And adding diethyl ether into the entered simulated wastewater to ensure that the mass concentration of the simulated wastewater is 35mg/L, culturing for 2 weeks at 36.5-39.5 ℃, wherein the COD of the effluent reaches 81.7 percent of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added.
c) And adding diethyl ether into the entered simulated wastewater to ensure that the mass concentration of the simulated wastewater is 15mg/L, culturing for 2 weeks at 36.5-39.5 ℃, and ensuring that the COD of the effluent reaches over 90.4 percent of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added.
d) And gradually increasing the mass concentration of the ether in the simulated wastewater to 120mg/L in the later period, wherein the adding amount of the ether in each time is 1.3-2.5 times of that in the previous period, and the culture period is 4 weeks.
Finally, the EGSB anaerobic reactor runs stably, the COD of the effluent of the EGSB anaerobic reactor reaches more than 91.2% of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added, the strain acclimation for improving the activity of the anaerobic granular sludge is completed, and the volcanic rock filler attached with the strain for improving the activity of the anaerobic granular sludge is obtained.
Wherein, in step 2), the volcanic stone filler 10 is the globular volcanic stone filler after polishing through the screening, and has a plurality of pinhole on the volcanic stone filler, the particle diameter of volcanic stone filler is 2~ 6mm, and the proportion is 1.01 ~ 1.10g/cm3, and the hollow rate is 48% ~ 60%, and the volcanic stone filler surface is crude, and difficult jam, and even the higher upward flow velocity of water conservancy in EGSB anaerobic reactor cuts the volcanic stone filler, and the biomembrane is difficult for droing. Meanwhile, the specific gravity of the volcanic rock is slightly heavier than that of water, and the volcanic rock can be in a suspension state in an environment with higher water lifting force and is uniformly distributed in the expanded anaerobic granular sludge as shown in figure 2.
Specifically, as shown in fig. 1 and 2, the EGSB anaerobic reactor comprises an EGSB main body reactor 2, a sampling port 4, a feed port 5, a wet gas flowmeter 6, a reactor outlet 7, a three-phase separator 8, and a circulation pump 9.
The water distribution tank 1 is communicated with the bottom in the EGSB main body reactor 2 through a water inlet pump 3. The side surface of the EGSB main body reactor 2 is provided with a reactor outlet 7, a feed inlet 5 and a sampling port 4 from top to bottom in sequence. The outlet 7 of the reactor is divided into two paths, one path is connected with the outside, and the other path is communicated with the bottom in the EGSB main body reactor 2 through a circulating pump 9. A three-phase separator 8 is arranged at the top inside the EGSB main reactor 2. A wet gas flow meter 6 is arranged at the top outside the EGSB main body reactor 2.
2 EGSB anaerobic reactors with the same volume are set as a test group and a control group, the volcanic rock filler 10 attached with the strain for improving the anaerobic granular sludge activity, which is obtained by the strain acclimation method for improving the anaerobic granular sludge activity, is added into the EGSB anaerobic reactors of the test group, the adding amount is 7 percent of the total volume of the EGSB anaerobic reactors, and the control group is not added.
Then anaerobic granular sludge 12 of a citric acid plant is respectively inoculated in the EGSB anaerobic reactors of the test group and the control group, the inlet water is 3 times of water diluted by alcohol-containing wastewater of a chemical plant, the EGSB anaerobic reactors of the test group and the control group are simultaneously started at the temperature of 36.5-39.5 ℃, the hydraulic retention time is set for 20h, and the rising flow rate is 7 m/h.
Wherein the inoculation amount of anaerobic granular sludge in a certain citric acid plant is 50 percent of the total volume of the EGSB reactor, and the sludge biomass expressed by the concentration of volatile suspended solids VSS is 58.4 g/L; the sedimentation rate is 124 m/h; the grain diameter of the anaerobic granular sludge 12 is 0.3-3 mm; the activity of the anaerobic granular sludge 12 is 321mgN g-1VSS d-1.
Wherein, certain chemical plant contains alcohol waste water, and the quality of water condition: COD 31200 mg/L; pH 3.4; ammonia nitrogen is 44.25 mg/L; 13.547mg/L total phosphorus; ethers 0.42%; the B/C ratio is 0.22, and mainly contains alcohols, citric acid, etc.
After the EGSB anaerobic reactor test group and the control group run for 3 weeks, the system effluent is stable, COD (chemical oxygen demand) and anaerobic granular sludge activity of the test group and the control group are continuously measured for one week, the COD mean value of the test group effluent is 1630mg/L, and the mean value of the granular sludge activity is 314mgN g-1VSS d-1; the COD mean value of effluent of the control group is 2150mg/L, and the activity mean value of the granular sludge is 272mgN g-1VSS d-1.
As can be seen from the comparison between the test group and the control group, the volcanic rock filler attached with the strain for improving the activity of the anaerobic granular sludge is added into the EGSB anaerobic reactor to treat the alcohol-containing wastewater, the COD of the effluent is reduced by 24.2 percent compared with that of the unadditized wastewater, and the activity of the granular sludge is increased by 13.4 percent compared with that of the unadditized wastewater.
Therefore, the strain capable of improving the activity of the granular sludge is added into the EGSB anaerobic reactor for treating the organic wastewater containing toxic substances, the COD concentration of the effluent can be reduced, the activity of the anaerobic granular sludge is improved, and the organic treatment load of the EGSB anaerobic reactor is improved.
The embodiments of the present invention are described only for the preferred embodiments of the present invention, and not for the limitation of the concept and scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the design concept of the present invention shall fall into the protection scope of the present invention, and the technical content of the present invention which is claimed is fully set forth in the claims.
Claims (10)
1. A strain domestication method for improving the activity of anaerobic granular sludge is characterized by comprising the following steps:
1) preparing simulated wastewater: the simulated wastewater comprises 2000mg/L of carbon source, 20mg/L of urea, 4mg/L of monopotassium phosphate, 50 mu g/L of quicklime, 62.5 mu g/L of magnesium sulfate and 25 mu g/L of trace elements, and the solvent is fresh water;
2) adding the volcanic rock filler into an EGSB anaerobic reactor;
3) then putting one part of the simulated wastewater prepared in the step 1) into a water distribution tank, putting the other part into an EGSB anaerobic reactor, inoculating hydrolytic acidification facultative anaerobic activated sludge in the EGSB anaerobic reactor at the temperature of 36.5-39.5 ℃, and externally circulating the simulated wastewater which does not enter the water distribution tank for 2-3 days through a circulating pump;
4) the EGSB anaerobic reactor starts to continuously feed the simulated wastewater in the distribution water tank, and the residual hydrolytic acidification facultative anaerobic activated sludge in the EGSB anaerobic reactor and on the surface of the volcanic rock filler is taken away by means of higher hydraulic power rising flow velocity;
5) adding benzene or ether toxic substances into the entered simulated wastewater;
finally, the EGSB anaerobic reactor runs stably, the COD of the effluent of the EGSB anaerobic reactor reaches more than 90% of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added, the strain acclimation for improving the activity of the anaerobic granular sludge is completed, and the volcanic rock filler attached with the strain for improving the activity of the anaerobic granular sludge is obtained.
2. The strain acclimatization method for improving anaerobic granular sludge activity according to claim 1, wherein the carbon source in step 1) is provided by glucose, or sodium acetate, or citric acid, or ethanol.
3. The strain acclimatization method for improving anaerobic granular sludge activity according to claim 1, wherein in the step 2), the vesuvianite filler is a spherical vesuvianite filler which is subjected to screening and polishing, a plurality of pinholes are formed in the vesuvianite filler, the grain size of the vesuvianite filler is 2-6 mm, and the specific gravity of the vesuvianite filler is 1.01-1.10 g/cm3The hollow rate is 48-60%, and the surface of the volcanic rock filler is rough.
4. The strain acclimation method for improving anaerobic granular sludge activity according to claim 1, wherein in the step 2), the adding amount of the vesuvianite filler is 30-60% of the total volume of the EGSB anaerobic reactor.
5. The strain acclimatization method for improving the activity of anaerobic granular sludge according to claim 1, wherein the hydrolysis acidification facultative anaerobic activated sludge in the step 3) is obtained from a hydrolysis acidification tank of a municipal or sewage treatment plant, the sludge concentration is 8-12 g/L, and the sludge settlement ratio is 30-60%;
in the step 3), the inoculation amount of the hydrolytic acidification facultative anaerobic activated sludge accounts for 60-80% of the total volume of the EGSB anaerobic reactor.
6. The strain acclimatization method for improving the activity of anaerobic granular sludge according to claim 1, wherein in the step 4), the EGSB anaerobic reactor starts to continuously feed simulated wastewater in the water tank, the hydraulic retention time is 10-30 h, the ascending flow rate is 4-10 m/h, and the operation lasts for 2-3 days; meanwhile, residual hydrolytic acidification facultative anaerobic activated sludge in the EGSB anaerobic reactor and on the surface of the volcanic rock filler is taken away by means of higher hydraulic power rising flow velocity, so that dead sludge enrichment is prevented.
7. The strain acclimation method for improving anaerobic granular sludge activity according to claim 1, wherein the method for adding benzene or ether toxic substances into the simulated wastewater in the step 5) comprises the following steps:
a) benzene or ether toxic substances are added into the entered simulated wastewater for the first time to ensure that the mass concentration of the benzene or ether toxic substances is 60mg/L, the wastewater is cultured for 3-4 weeks at the temperature of 36.5-39.5 ℃, and the COD of the effluent of the EGSB anaerobic reactor reaches 65-75% of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added;
b) then benzene or ether toxic substances are added into the entered simulated wastewater to ensure that the mass concentration of the benzene or ether toxic substances is 35mg/L, the wastewater is cultured for 2-3 weeks at the temperature of 36.5-39.5 ℃, and the COD of the effluent of the EGSB anaerobic reactor reaches 75-85% of the COD of the effluent of the EGSB anaerobic reactor when no toxic substances are added;
c) then benzene or ether toxic substances are added into the entered simulated wastewater to ensure that the mass concentration is 15mg/L, the wastewater is cultured for 2 weeks at the temperature of 36.5-39.5 ℃, and the COD of the effluent reaches more than 90 percent of the COD of the effluent of the EGSB anaerobic reactor when the toxic substances are not added;
d) and gradually increasing the mass concentration of the benzene or ether toxic substances in the entered simulated wastewater to 120mg/L in the later period, wherein the adding amount is 1.3-2.5 times of that of the toxic substances in the simulated wastewater each time, and the culture period is 3-5 weeks.
8. The strain acclimatization method for improving anaerobic granular sludge activity according to claim 1, wherein the ether toxic substance in the step 5) is ethyl ether.
9. The strain acclimatization method for improving anaerobic granular sludge activity according to claim 1, wherein the EGSB anaerobic reactor comprises an EGSB main body reactor, a sampling port, a feeding port, a wet gas flowmeter, a reactor outlet, a three-phase separator and a circulating pump;
the water distribution tank is communicated with the bottom in the EGSB main body reactor through a water inlet pump, the side surface of the EGSB main body reactor is sequentially provided with a reactor outlet, a feed inlet and a sampling port from top to bottom, the reactor outlet is divided into two paths, one path is connected with the outside, the other path is communicated with the bottom in the EGSB main body reactor through a circulating pump, the three-phase separator is arranged at the top in the EGSB main body reactor, and the wet gas flowmeter is arranged at the top outside the EGSB main body reactor.
10. The application of the volcanic rock filler attached with the strain for improving the activity of the anaerobic granular sludge, which is obtained by the strain acclimation method for improving the activity of the anaerobic granular sludge in claim 1, is characterized by comprising the following steps:
adding the volcanic rock filler attached with the strain for improving the activity of the anaerobic granular sludge into the EGSB anaerobic reactor for treating the organic wastewater containing toxic substances, wherein the adding amount is 1-15% of the total volume of the EGSB anaerobic reactor.
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