CN110156170B - Biological growth promoter for sewage treatment - Google Patents
Biological growth promoter for sewage treatment Download PDFInfo
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- CN110156170B CN110156170B CN201810256813.5A CN201810256813A CN110156170B CN 110156170 B CN110156170 B CN 110156170B CN 201810256813 A CN201810256813 A CN 201810256813A CN 110156170 B CN110156170 B CN 110156170B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The invention discloses a biological growth promoter for sewage treatment, which consists of an agent A and an agent B, is matched according to the mass ratio (1-2) to 20, and comprises the following components in percentage by mass: 2-6% of complex enzyme preparation, 10-15% of compound amino acid, 6-8% of compound vitamin, 1-5% of adenine, 2-6% of lecithin, 6-10% of glycerol, 20-28% of cottonseed cake meal hydrolysate, 10-18% of corn steep liquor and the balance of phosphate buffer salt solution; and (2) agent B: 15-22% of organic acid, 8-12% of sodium succinate, 25-30% of methanol, 3-6% of ferriporphyrin, 8-12% of potassium fulvate, 3-6% of pyridoxal phosphate, 2-6% of manganese chloride, 2-5% of copper sulfate and the balance of sterile water. The bio-stimulant disclosed by the invention is low in consumption, beneficial microorganisms can be rapidly enriched, and the activity and concentration of sludge can be rapidly improved, so that the sewage treatment efficiency can be improved, the impact resistance of a system can be improved, the sludge domestication speed can be increased, the damaged activated sludge can be rapidly repaired, and the debugging time of the system can be shortened; the tolerance of the wastewater biochemical treatment system to chemical substances is improved, and the adaptability of microorganisms to temperature and salinity is improved.
Description
Technical Field
The invention belongs to the technical field of biochemical treatment of wastewater, and particularly relates to a biological growth promoter for sewage treatment.
Background
The biochemical treatment of waste water aims at eliminating organic matter, nitrogen and phosphorus elements and colloid solid matter incapable of being naturally precipitated. According to the reports of literature data, about more than 60% of organic matters in municipal sewage are most economical to remove only by a biological method, while the removal of nitrogen in wastewater generally depends on the biological method, more than 90% of the municipal sewage treatment plants established in the world currently apply a biological treatment method, and most industrial sewage treatment plants mainly adopt the biological method.
The biochemical treatment of wastewater is to treat pollutants in wastewater by the metabolism of microorganisms, and can be generally divided into two categories, namely aerobic treatment and anaerobic treatment. However, when the water body is seriously polluted and the bottom mud contains more organic substances and toxic substances, beneficial microorganisms in the system are inhibited, so that the effect of decomposing pollutants cannot be achieved, even the number of organisms is reduced, and the water body ecology is unbalanced; in the wastewater treatment, the sludge activity is reduced or a large impact is applied due to factors such as water quality fluctuation and oxygen deficiency, and the sludge cannot be self-repaired in a short time.
Therefore, the bio-growth promoting technology is suitable for growth, the investment is low, the energy consumption is low, the management is convenient, and the bio-growth promoting technology is safe and reliable, but most of the existing bio-growth promoting agents are bio-nutrients, the bio-nutrients provided by the bio-growth promoting agents are single, the bio-growth promoting agent has limited effect when being used for treating water bodies with complex and difficult degradation of pollutants and sewage treatment systems with seriously unbalanced ecology, the improvement and restoration time is long, and the influence on promoting the ecological structure to be highly evolved is small.
Disclosure of Invention
Based on the technical problems existing in the background technology, the invention provides a bio-stimulant for sewage treatment, which can improve or recover the number of beneficial flora in a biochemical system in a short time, so that the biochemical system forms a higher-level ecological structure to improve the shock resistance of the system, thereby improving the degradation effect of the system on COD and ammonia nitrogen.
The invention provides a biological growth promoter for sewage treatment, which consists of an agent A and an agent B, is used in a matching way according to the mass ratio (1-2) to 20, and comprises the following components in percentage by mass:
an agent A: 2-6% of complex enzyme preparation, 10-15% of compound amino acid, 6-8% of compound vitamin, 1-5% of adenine, 2-6% of lecithin, 6-10% of glycerol, 20-28% of cottonseed cake meal hydrolysate, 10-18% of corn steep liquor and the balance of phosphate buffer salt solution.
And (2) agent B: 15-22% of organic acid, 8-12% of sodium succinate, 25-30% of methanol, 3-6% of ferriporphyrin, 8-12% of potassium fulvate, 3-6% of pyridoxal phosphate, 2-6% of manganese chloride, 2-5% of copper sulfate and the balance of sterile water.
Preferably, the complex enzyme preparation comprises the following components in percentage by mass: 30% of laccase, 30% of cellulase, 20% of saccharifying enzyme and 20% of amylase.
Preferably, the compound amino acid comprises the following components in percentage by mass: 35% of L-histidine, 35% of L-tyrosine and 30% of L-methionine.
Preferably, the vitamin complex comprises the following components in percentage by mass: 35% of biotin, 30% of riboflavin and 35% of nicotinamide.
Preferably, the formulation of the phosphate buffered saline solution is as follows: 8g/L of sodium chloride, 0.2g/L of potassium chloride, 0.27g/L of potassium dihydrogen phosphate and 1.42g/L of disodium hydrogen phosphate, and the solvent is deionized water.
Preferably, the organic acid comprises the following components in percentage by mass: 20% of pyruvic acid, 50% of malic acid, 15% of alpha-ketoglutaric acid and 15% of oxaloacetic acid.
The preparation method of the biological growth promoter for sewage treatment comprises the following steps:
s1, sterilizing glycerol, cottonseed cake meal hydrolysate and corn steep liquor by high-pressure steam, adding into a stirring container, cooling to 40 ℃, sequentially adding phosphate buffer salt solution, complex enzyme preparation, complex amino acid, complex vitamin, adenine and lecithin, and stirring at normal temperature and normal pressure for 30min at a speed of 50r/min to obtain the preparation A.
S2, sterilizing tap water by high-pressure steam, cooling to 40 ℃ to obtain sterile water, adding the sterile water into a stirring container, sequentially adding methanol, organic acid, sodium succinate, ferriporphyrin, potassium fulvate, pyridoxal phosphate, manganese chloride and copper sulfate, and stirring at normal temperature and normal pressure for 30min at 50r/min to obtain the preparation B.
Preferably, the autoclaving conditions of the glycerol, the cottonseed meal hydrolysate and the corn steep liquor in the step S1 are 115 ℃ for 30min, and the autoclaving conditions of the tap water in the step S2 are 121 ℃ for 15 min.
The application method of the biological growth promoter for sewage treatment comprises the following steps: adding the agent B, and adding the agent A after 20min, wherein the adding points of the agent A and the agent B are both at the water inlet end, the dosage of the agent A is 2g/t-5g/t, and the dosage of the agent B is 20g/t-100 g/t.
The innovation points of the invention are as follows:
1. the growth promoter consists of an agent A and an agent B, wherein the agent A mainly consists of biomacromolecules and multiple vitamins, the part is easy to inactivate, and the agent B consists of a carbon source required by microorganisms and trace elements in a microorganism metabolic pathway. The existing growth promoter in the market mixes substances such as vitamins, enzymes and the like with various metals and organic acid substances, so that various components are easily inactivated or the shelf life is short. The agent A and the agent B can respectively form a buffer system, so that the quality guarantee period is greatly prolonged.
2. The invention aims at important metabolites in TCA metabolic pathway, lipid metabolic pathway and electronic respiration transfer chain pathway of the microorganism to promote growth so as to strengthen the metabolic action and the respiration action of the microorganism, thereby achieving the purposes of rapid propagation and strong impact resistance of the microorganism.
3. The pyruvic acid, oxaloacetic acid, ferriporphyrin, pyridoxal phosphate, lecithin and the like used in the invention are not common substances for activating the strains, but can play a role in promoting growth by cooperating with other components.
The invention has the beneficial effects that: the biological growth promoter controls C to N to P through different adding proportions of the agent A and the agent B, and can be better suitable for biochemical treatment of various industrial wastewater and domestic wastewater such as coking wastewater, papermaking wastewater, textile printing and dyeing wastewater, chemical wastewater and the like which are difficult to degrade. The bio-stimulant comprises enzyme, amino acid, organic acid, carbon source, nitrogen source, vitamin, trace elements and a buffer system, is low in dosage, is beneficial to rapid enrichment of beneficial microorganisms, and rapidly improves the activity and concentration of sludge, thereby improving the efficiency of a sewage treatment system; the shock resistance of the system is improved; the sludge domestication speed is improved, the damaged activated sludge can be quickly repaired, and the debugging time of the system is shortened; the tolerance of the wastewater biochemical treatment system to chemical substances is improved, and the adaptability of microorganisms to temperature and salinity is improved.
After the biological growth promoter is used, the removal rate of COD and ammonia nitrogen in sewage can reach more than 90%.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
The contents of the components of the agent A and the agent B are shown in Table 1.
TABLE 1 weight ratio of the components of agent A and agent B
The preparation method of the biological growth promoter comprises the following steps: sterilizing glycerol, cottonseed cake meal hydrolysate and corn steep liquor by high-pressure steam at 115 ℃ for 30min according to the proportion, adding into a stirring container, cooling to 40 ℃ 9, sequentially adding phosphate buffer solution, complex enzyme preparation, complex amino acid, complex vitamin, adenine and lecithin, and stirring at normal temperature and normal pressure for 30min at 50r/min to obtain the agent A.
Sterilizing tap water at 121 deg.C under high pressure for 15min, cooling to 40 deg.C to obtain sterile water, adding into stirring container, sequentially adding methanol, organic acid, sodium succinate, ferriporphyrin, potassium fulvate, pyridoxal phosphate, manganese chloride, and copper sulfate, and stirring at normal temperature and pressure for 30min at a speed of 50r/min to obtain preparation B.
The prepared biological growth promoting agent is applied in the following mode: after the agent B is added into the biochemical treatment tank for 20min, the agent A is added, and the specific addition amount is shown in Table 2.
TABLE 2 dosage amounts of agent A and agent B
| Examples | 1 | 2 | 3 | 4 | 5 |
| Kind of waste water | Domestic sewage | Coking wastewater | Printing and dyeing wastewater | Papermaking wastewater | Coal chemical wastewater |
| Agent A | 2g/t | 5g/t | 4g/t | 3g/t | 5g/t |
| Agent B | 20g/t | 60g/t | 80g/t | 36g/t | 100g/t |
Physical and chemical index detection
COD and ammonia nitrogen of inlet water and outlet water treated by the biological growth promoting agent of the invention in examples 1-5 are detected by adopting standard HJ535-2009 and HJ828-2017, and the detection results are shown in Table 3:
TABLE 3 test results
The COD and ammonia nitrogen removal rates of the original biochemical treatment and the biochemical treatment after adding the biological growth promoting agent are compared and shown in the table 4:
TABLE 4 comparison of COD and Ammonia Nitrogen removal
From table 4, it can be seen that the bio-stimulant of the present invention can significantly enhance the degradation of domestic sewage, coking wastewater, printing and dyeing wastewater, papermaking wastewater, coal chemical wastewater COD, ammonia nitrogen by a biochemical treatment system.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (1)
1. A biological growth promoter for sewage treatment is characterized by consisting of an agent A and an agent B, which are matched according to a mass ratio (1-2) to 20, and comprise the following components in percentage by mass:
an agent A: 2-6% of complex enzyme preparation, 10-15% of compound amino acid, 6-8% of compound vitamin, 1-5% of adenine, 2-6% of lecithin, 6-10% of glycerol, 20-28% of cottonseed cake meal hydrolysate, 10-18% of corn steep liquor and the balance of phosphate buffer salt solution;
and (2) agent B: 15-22% of organic acid, 8-12% of sodium succinate, 25-30% of methanol, 3-6% of ferriporphyrin, 8-12% of potassium fulvate, 3-6% of pyridoxal phosphate, 2-6% of manganese chloride, 2-5% of copper sulfate and the balance of sterile water;
the compound enzyme preparation comprises the following components in percentage by mass: 30% of laccase, 30% of cellulase, 20% of glucoamylase and 20% of amylase;
the compound amino acid comprises the following components in percentage by mass: 35% of L-histidine, 35% of L-tyrosine and 30% of L-methionine;
the compound vitamin comprises the following components in percentage by mass: 35% of biotin, 30% of riboflavin and 35% of nicotinamide;
the phosphate buffer solution comprises the following formula: 8g/L of sodium chloride, 0.2g/L of potassium chloride, 0.27g/L of monopotassium phosphate and 1.42g/L of disodium hydrogen phosphate, and the solvent is deionized water;
the organic acid comprises the following components in percentage by mass: 20% of pyruvic acid, 50% of malic acid, 15% of alpha-ketoglutaric acid and 15% of oxaloacetic acid;
the preparation method of the biological growth promoter comprises the following steps:
s1, sterilizing glycerol, cottonseed cake meal hydrolysate and corn steep liquor by high-pressure steam, adding into a stirring container, cooling to 40 ℃, sequentially adding phosphate buffer salt solution, complex enzyme preparation, complex amino acid, complex vitamin, adenine and lecithin, and stirring at normal temperature and normal pressure for 30min at 50r/min to obtain agent A;
s2, sterilizing tap water by high-pressure steam, cooling to 40 ℃ to obtain sterile water, adding the sterile water into a stirring container, sequentially adding methanol, organic acid, sodium succinate, ferriporphyrin, potassium fulvate, pyridoxal phosphate, manganese chloride and copper sulfate, and stirring at normal temperature and normal pressure for 30min at 50r/min to obtain a preparation B;
the high-pressure steam sterilization condition of the glycerol, the cottonseed cake meal hydrolysate and the corn steep liquor in the step S1 is 115 ℃ for 30min, and the high-pressure steam sterilization condition of the tap water in the step S2 is 121 ℃ for 15 min;
the use method of the biological growth promoting agent comprises the following steps:
adding the agent B, and adding the agent A after 20min, wherein the adding points of the agent A and the agent B are both at the water inlet end, the dosage of the agent A is 2g/t-5g/t, and the dosage of the agent B is 20g/t-100 g/t.
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| CN114835267B (en) * | 2022-05-24 | 2023-05-30 | 浙江桃花源环保科技有限公司 | Microbial activity promoting method for biological wastewater treatment |
| CN114958812B (en) * | 2022-06-27 | 2023-04-07 | 上海交通大学 | Method for promoting sludge feed conversion by using complex enzyme |
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| US5015385A (en) * | 1986-03-13 | 1991-05-14 | Btc Biotechnik International Gmbh | Process for promotion of microbial consumption of waste water fats |
| WO2004108609A1 (en) * | 2003-06-06 | 2004-12-16 | Forskningscenter Risø | Fermentation media comprising wastewater and use hereof |
| CN101736031A (en) * | 2008-11-06 | 2010-06-16 | 山东沃德生物技术有限公司 | Fat-soluble biological composite fermentation accelerator and application thereof |
| CN102198980A (en) * | 2010-03-24 | 2011-09-28 | 同济大学 | Biostimulation agent and preparation method thereof |
| CN102515364A (en) * | 2011-11-03 | 2012-06-27 | 袁磊 | Broad-spectrum bio-energizer for industrial wastewater treatment and preparation method thereof |
| CN106256773A (en) * | 2016-08-09 | 2016-12-28 | 湖北凌卓生物工程有限公司 | A kind of Biostimulation agent rapidly promoting microbial reproduction |
| CN106830358A (en) * | 2017-02-21 | 2017-06-13 | 佛山市碧沃丰生物科技股份有限公司 | A kind of sewage disposal biological growth promoter and preparation method thereof, applying method |
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- 2018-03-27 CN CN201810256813.5A patent/CN110156170B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5015385A (en) * | 1986-03-13 | 1991-05-14 | Btc Biotechnik International Gmbh | Process for promotion of microbial consumption of waste water fats |
| WO2004108609A1 (en) * | 2003-06-06 | 2004-12-16 | Forskningscenter Risø | Fermentation media comprising wastewater and use hereof |
| CN101736031A (en) * | 2008-11-06 | 2010-06-16 | 山东沃德生物技术有限公司 | Fat-soluble biological composite fermentation accelerator and application thereof |
| CN102198980A (en) * | 2010-03-24 | 2011-09-28 | 同济大学 | Biostimulation agent and preparation method thereof |
| CN102515364A (en) * | 2011-11-03 | 2012-06-27 | 袁磊 | Broad-spectrum bio-energizer for industrial wastewater treatment and preparation method thereof |
| CN106256773A (en) * | 2016-08-09 | 2016-12-28 | 湖北凌卓生物工程有限公司 | A kind of Biostimulation agent rapidly promoting microbial reproduction |
| CN106830358A (en) * | 2017-02-21 | 2017-06-13 | 佛山市碧沃丰生物科技股份有限公司 | A kind of sewage disposal biological growth promoter and preparation method thereof, applying method |
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