CN106279387A - A kind of grass leaf protein extraction process - Google Patents

A kind of grass leaf protein extraction process Download PDF

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Publication number
CN106279387A
CN106279387A CN201610649381.5A CN201610649381A CN106279387A CN 106279387 A CN106279387 A CN 106279387A CN 201610649381 A CN201610649381 A CN 201610649381A CN 106279387 A CN106279387 A CN 106279387A
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leaf protein
grass leaf
herbage
extraction process
grass
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阿苏尔坡
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Yuexi County Green Fields Of Modern Ecological Agriculture Development Co Ltd
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Yuexi County Green Fields Of Modern Ecological Agriculture Development Co Ltd
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Priority to CN202210523477.2A priority Critical patent/CN114805516A/en
Priority to CN201610649381.5A priority patent/CN106279387A/en
Publication of CN106279387A publication Critical patent/CN106279387A/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/40—Treatment of liquids or slurries
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/004—Liquid waste from mechanical processing of material, e.g. wash-water, milling fluid, filtrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y115/00—Oxidoreductases acting on superoxide as acceptor (1.15)
    • C12Y115/01—Oxidoreductases acting on superoxide as acceptor (1.15) with NAD or NADP as acceptor (1.15.1)
    • C12Y115/01001—Superoxide dismutase (1.15.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Mechanical Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention provides a kind of grass leaf protein extraction process, belong to vegetable protein extractive technique field, it comprises the following steps: pretreatment, centrifugal filtration, ultra-filtration and separation, is concentrated by ultrafiltration and is spray-dried.This grass leaf protein extraction process passes sequentially through pretreatment, centrifugal filtration, ultra-filtration and separation, ultrafiltration concentration and spray drying step and extracts from fresh herbage and obtain grass leaf protein; therefore; improve the added value of herbage; development for pasture industry provides wide space, and provides precondition for scale, the grass leaf protein construction of production base of industrialization.

Description

A kind of grass leaf protein extraction process
Technical field
The present invention relates to vegetable protein extractive technique field, in particular to a kind of grass leaf protein extraction process.
Background technology
Along with developing rapidly of animal husbandry and feed industry, the contradiction that protein feeds is not enough is the most prominent, how to open Send out the protein new feedstuff resource utilizing good quality and high output, be to put the most urgent but also difficult task in face of us, after current herbage Continuous purchase intensive processing problem seriously governs the development of Forage grass industry, and the outlet solving this problem is only high-quality to be herded Grass elite leaf protein extracts, and as pig, the high protein feed of fowl, using remaining grass slag as ruminant feed, enters Row intensive processing, promotes grass cultivation added value.
Since the nineties, by substantial amounts of research and production practices, the production technology of leaf protein feedstuff is selected from raw material Select, pulverize squeezing, condense and separate, be dried and the aspect such as work continuously of each procedure all achieves bigger improvement.
But, owing to the process technology of plant leaf protein matter is fallen behind by China, the most high factor of economic benefit affects, and causes Plant leaf protein Quality Research focuses primarily upon the research of laboratory method, and to extracting the raw material of leaf protein, extracting method And affect the research of the aspects such as leaf protein extraction ratio, not yet accomplish scale production.
Summary of the invention
In order to solve the problems referred to above, it is an object of the invention to provide the leaf protein extraction process of a kind of herbage, to promote Herbage added value, the development for pasture industry provides wide space, and the grass leaf protein for scale, industrialization produces base Ground is built and is provided precondition.
The technical solution adopted in the present invention is:
A kind of grass leaf protein extraction process, it comprises the following steps:
(1) pretreatment: take that fresh herbage is the most cleaned, guillotine cutting, pulverize and after making beating processes, be prepared as serosity, so After add and soften water and be prepared as the feed liquid of pulpous state；
(2) centrifugal filtration: respectively obtain herbage juice and herbage slag after the feed liquid in step (1) being filtered by centrifugation；
(3) ultra-filtration and separation: taking the herbage juice in step (2), isolated contains the filter of grass leaf protein after ultrafiltration Liquid；Described filtrate respectively obtains grass leaf protein extracting solution and residue liquid again after membrance separation；
(4) it is concentrated by ultrafiltration: take the grass leaf protein extracting solution in step (3), is concentrated after membrance concentration system concentrates Liquid；
(5) it is spray-dried: the concentrated solution in step (4) is spray-dried, prepares grass leaf protein after drying.
Further, the described herbage slag drying in step (2) makes grass particle.
Further, the described residue liquid in step (3) is used for preparing fertilizer after microbe inoculation bacterium.
Further, step (3) also includes SOD enzyme preparation process: takes described grass leaf protein extracting solution and carries out adsorption column suction Attached process, and the SOD enzyme in described grass leaf protein is adsorbed in adsorption column；Wherein, the SOD enzyme warp successively of adsorption column it is adsorbed in SOD enzyme is prepared after eluting, concentration, drying steps；Described grass leaf protein extracting solution after adsorption column processes carries out institute State the subsequent step of grass leaf protein extraction process.
Further, described herbage is Hairy vetch herbage or Radix rumicis acetosae herbage.
Wherein, Hairy vetch herbage is preferably the Hairy vetch herbage of Goats in Liangshan Prefecture, and it has aboundresources, quality better Etc. advantage, can preferably be applicable to this grass leaf protein extraction process.
Beneficial effects of the present invention:
Grass leaf protein extraction process provided by the present invention, passes sequentially through pretreatment, centrifugal filtration, ultra-filtration and separation, surpasses Filter concentrates and spray drying step extracts from fresh herbage and obtains grass leaf protein, therefore, improves the added value of herbage, Development for pasture industry provides wide space, and the grass leaf protein construction of production base for scale, industrialization provides Precondition.
Accompanying drawing explanation
Fig. 1 is the Hairy vetch grass leaf protein extraction process flow chart described in embodiment 1；
Fig. 2 is the Hairy vetch grass leaf protein extraction process flow chart described in embodiment 2.
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below will be in the embodiment of the present invention Technical scheme be clearly and completely described, it is clear that described embodiment be a part of embodiment of the present invention rather than Whole embodiments.
Therefore, detailed description to embodiments of the invention is not intended to limit the model of claimed invention below Enclose, but be merely representative of the selected embodiment of the present invention.Based on the embodiment in the present invention, those of ordinary skill in the art are not having Have and make the every other embodiment obtained under creative work premise, broadly fall into the scope of protection of the invention.
Embodiment 1
As it is shown in figure 1, present embodiments provide a kind of Hairy vetch grass leaf protein extraction process, it includes following step Rapid:
Take fresh Hairy vetch herbage, first that it is the most cleaned, guillotine cutting, pulverize and making beating process after be prepared as Serosity, adds softening water and is prepared as the feed liquid of pulpous state, and wherein, serosity is 1:4 with the volume ratio softening water of addition, makes light leaf Leaf protein in Hairy Vetch herbage is fully extracted.
Then Hairy vetch herbage juice and Hairy vetch herbage slag, light leaf are respectively obtained after feed liquid filters by centrifugation Hairy Vetch herbage slag is dried and is prepared as grass particle, for the foodstuff of the domestic animal such as cattle, sheep；Hairy vetch herbage juice is then through ultrafiltration Rear isolated Hairy vetch grass leaf protein extracting solution and residue liquid.
Wherein, may be used for preparing fertilizer after residue liquid microbe inoculation；Hairy vetch grass leaf protein is carried Taking liquid to be passed through membrance concentration system and concentrate, the concentrated solution after finally concentrating carries out being spray-dried and to prepare light leaf pale reddish brown Sweet potato grass leaf protein.
Therefore, this Hairy vetch grass leaf protein extraction process not only achieves carrying of Hairy vetch grass leaf protein Taking, improve the added value of herbage, the development for pasture industry provides wide space, and is scale, the herbage of industrialization Leaf protein construction of production base provides precondition；And it is produced Hairy vetch herbage slag in its extraction process, residual Slag liquid can also be fully utilized, it is to avoid the waste of resource, environmentally friendly.
It is spray-dried to make Hairy vetch grass leaf protein extracting solution be applicable to, it is preferable that Hairy vetch is herded Blade of grass protein extract is concentrated into the half of its original volume, the most i.e. eliminates part water, is applicable to again be spray-dried.
In the present embodiment, can use bag filter that Hairy vetch herbage juice is carried out ultrafiltration, it has filtration Treating capacity is big, side leakage rate is little, easy cleaning, low cost and use the advantage such as flexibly.
Embodiment 2
As in figure 2 it is shown, present embodiments provide the leaf protein extraction process of a kind of Hairy vetch herbage, it includes following Step:
Take fresh Hairy vetch herbage, first that it is the most cleaned, guillotine cutting, pulverize and making beating process after be prepared as Serosity, adds softening water and is prepared as the feed liquid of pulpous state, and wherein, serosity is 1:4 with the volume ratio softening water of addition, makes light leaf Leaf protein in Hairy Vetch herbage is fully extracted.
Then Hairy vetch herbage juice and Hairy vetch herbage slag, light leaf are respectively obtained after feed liquid filters by centrifugation Hairy Vetch herbage slag is dried and is prepared as grass particle, for the foodstuff of the domestic animal such as cattle, sheep；Hairy vetch herbage juice is then through ultrafiltration Rear isolated Hairy vetch grass leaf protein extracting solution and residue liquid.
May be used for preparing fertilizer again after residue liquid microbe inoculation；By Hairy vetch grass leaf protein extracting solution Add in adsorption column and carry out SOD enzyme absorption；Wherein, the Hairy vetch grass leaf protein extracting solution after adsorption column adsorption treatment It is passed through membrance concentration system to concentrate, and is concentrated into the half of original volume；Concentrated solution after finally concentrating is spray-dried And prepare Hairy vetch grass leaf protein；The SOD enzyme being adsorbed in adsorption column is then through eluting, concentration, lyophilization SOD enzyme product is obtained after process.
Therefore, this Hairy vetch grass leaf protein extraction process not only achieves carrying of Hairy vetch grass leaf protein Take, but also can therefrom separate and prepare SOD enzyme product, it is achieved that herbage intensive processing, preferably improve the attached of herbage Value added.
In above-described embodiment, the volume ratio softening water of serosity and addition including, but not limited to 1:4, but, if softening Water consumption is less and causes serosity Leaf Protein extraction unit abundant, causes waste；If soften water consumption too much, extraction effect without Significant change, not only waste water resource, but also add the difficulty of subsequent processing steps, therefore, its ratio is preferably 1:4- 6。
Leaf protein extraction process in above-described embodiment can be also used for Radix rumicis acetosae herbage, thus extracts the leaf in Radix rumicis acetosae herbage Albumen.
Embodiment 3
Use the Hairy vetch herbage leaf prepared by Hairy vetch grass leaf protein extraction process in embodiment 2 It is qualified that protein product analyzes inspection center's survey inspection through Sichuan Academy of Agricultural Sciences, and its assay is as shown in table 1 below.
Table 1 assay
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, that is made any repaiies Change, equivalent, improvement etc., should be included within the scope of the present invention.

Claims (5)

1. a grass leaf protein extraction process, it is characterised in that comprise the following steps:
Pretreatment: take that fresh herbage is the most cleaned, guillotine cutting, pulverize and after making beating processes, be prepared as serosity, then add Soften water and be prepared as the feed liquid of pulpous state；
Centrifugal filtration: respectively obtain herbage juice and herbage slag after the feed liquid in step (1) being filtered by centrifugation；
Ultra-filtration and separation: taking the herbage juice in step (2), isolated contains the filtrate of grass leaf protein after ultrafiltration；Described Filtrate respectively obtains grass leaf protein extracting solution and residue liquid again after membrance separation；
It is concentrated by ultrafiltration: take the grass leaf protein extracting solution in step (3), after membrance concentration system concentrates, obtains concentrated solution；
It is spray-dried: the concentrated solution in step (4) is spray-dried, prepares grass leaf protein after drying.
Grass leaf protein extraction process the most according to claim 1, it is characterised in that the described herbage slag in step (2) Drying makes grass particle.
Grass leaf protein extraction process the most according to claim 1 and 2, it is characterised in that the described residue in step (3) Liquid is used for preparing fertilizer after microbe inoculation bacterium.
Grass leaf protein extraction process the most according to claim 1 and 2, it is characterised in that step (3) also includes SOD enzyme Preparation process: take described grass leaf protein extracting solution and carry out adsorption column adsorption treatment, and by the SOD enzyme in described grass leaf protein It is adsorbed in adsorption column；Wherein, the SOD enzyme being adsorbed in adsorption column prepares SOD enzyme successively after eluting, concentration, drying steps； Described grass leaf protein extracting solution after adsorption column processes carries out the subsequent step of described grass leaf protein extraction process.
Grass leaf protein extraction process the most according to claim 1 and 2, it is characterised in that described herbage is that light leaf is pale reddish brown Sweet potato herbage or Radix rumicis acetosae herbage.
CN201610649381.5A 2016-08-09 2016-08-09 A kind of grass leaf protein extraction process Pending CN106279387A (en)

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CN202210523477.2A CN114805516A (en) 2016-08-09 2016-08-09 Pretreatment method and extraction process for extracting grass leaf protein
CN201610649381.5A CN106279387A (en) 2016-08-09 2016-08-09 A kind of grass leaf protein extraction process

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1943391A (en) * 2006-11-09 2007-04-11 武汉光盛生物工程技术开发有限公司 Composite extracting method for plant leaf protein and its use on hybrid giant napier
CN102450394A (en) * 2010-10-25 2012-05-16 郝明然 Extraction method of grass leaf protein
CN103289969A (en) * 2013-06-08 2013-09-11 中国科学院烟台海岸带研究所 Method for extracting superoxide dismutase from plant stem leaves
CN103509763A (en) * 2013-10-11 2014-01-15 中国科学院烟台海岸带研究所 Process method for extracting leaf protein and superoxide dismutase from plants
CN103509097A (en) * 2013-10-11 2014-01-15 中国科学院烟台海岸带研究所 Extraction process method for plant leaf protein

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003093464A1 (en) * 2002-05-06 2003-11-13 Genesis Research And Development Corporation Limited Compositions isolated from forage grasses and methods for their use

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1943391A (en) * 2006-11-09 2007-04-11 武汉光盛生物工程技术开发有限公司 Composite extracting method for plant leaf protein and its use on hybrid giant napier
CN102450394A (en) * 2010-10-25 2012-05-16 郝明然 Extraction method of grass leaf protein
CN103289969A (en) * 2013-06-08 2013-09-11 中国科学院烟台海岸带研究所 Method for extracting superoxide dismutase from plant stem leaves
CN103509763A (en) * 2013-10-11 2014-01-15 中国科学院烟台海岸带研究所 Process method for extracting leaf protein and superoxide dismutase from plants
CN103509097A (en) * 2013-10-11 2014-01-15 中国科学院烟台海岸带研究所 Extraction process method for plant leaf protein

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