CN106265843A - A kind of extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins - Google Patents
A kind of extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins Download PDFInfo
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- CN106265843A CN106265843A CN201610679992.4A CN201610679992A CN106265843A CN 106265843 A CN106265843 A CN 106265843A CN 201610679992 A CN201610679992 A CN 201610679992A CN 106265843 A CN106265843 A CN 106265843A
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- herba hylotelephii
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- 241001165596 Hylotelephium erythrostictum Species 0.000 title claims abstract description 82
- 229930182490 saponin Natural products 0.000 title claims abstract description 57
- 150000007949 saponins Chemical class 0.000 title claims abstract description 57
- 235000017709 saponins Nutrition 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 52
- 239000002904 solvent Substances 0.000 claims abstract description 35
- 230000008569 process Effects 0.000 claims abstract description 30
- 238000010298 pulverizing process Methods 0.000 claims abstract description 21
- 238000004821 distillation Methods 0.000 claims abstract description 17
- 230000006837 decompression Effects 0.000 claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 238000001035 drying Methods 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 3
- 230000001476 alcoholic effect Effects 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 25
- 238000004904 shortening Methods 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 23
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 15
- 238000010025 steaming Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000002386 leaching Methods 0.000 description 4
- 238000003811 acetone extraction Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 235000008216 herbs Nutrition 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000002137 ultrasound extraction Methods 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 208000034507 Haematemesis Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 201000008197 Laryngitis Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 235000003143 Panax notoginseng Nutrition 0.000 description 1
- 241000180649 Panax notoginseng Species 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- 208000024780 Urticaria Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 210000003026 hypopharynx Anatomy 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000004371 toothache Diseases 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/41—Crassulaceae (Stonecrop family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
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Abstract
The present invention provides the extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins, it is characterised in that including: dried by Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti, pulverize；Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after soaked in solvent pulverizing, obtains soak；By described soak supersound process；Product after described supersound process is filtered, takes filter liquor；By the decompression distillation of described filter liquor, take concentrated solution；Described concentrated solution is dried；Wherein, described Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is (5～20) with the weight ratio of described solvent: 1.The extracting method of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins that the present invention provides, simple to operate, while shortening extraction time, improve the extraction ratio of total saponins.
Description
Technical field
The present invention relates to active ingredient of Chinese herbs and extract field, be specifically related to the extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins.
Background technology
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti, is commonly called as Radix Notoginseng of invigorating blood circulation, and has its record as medical material among the people in Shennong's Herbal, with entirely
Grass is used as medicine, and the whole year can adopt, many using fresh herbs.Have the effects such as promoting blood circulation and hemostasis, expelling wind and removing dampness, heat-clearing and toxic substances removing, interior treatment laryngitis, haematemesis,
Urticaria, mastitis etc., the diseases such as traumatic injury, hypertension, laryngopharynx swelling and pain, toothache are controlled in external.Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins is as eight
The active substance that in precious Herba hylotelephii erythrosticti, a class is important, can improve the activity of the antioxidases such as internal SOD, CAT, thus enhancing body antioxygen
Change systemic-function, prevent the infringement to body of the too much oxygen-derived free radicals.
In prior art, the total saponins used in microwave loss mechanisms and acetone extraction extraction Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti more, but these
In method, the extraction ratio of total saponins all ratios are relatively low.
Summary of the invention
For defect of the prior art, it is desirable to provide the extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins, operation
Simply, while shortening extraction time, improve the extraction ratio of total saponins.
For solving the problems referred to above, the present invention provides the extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins, it is characterised in that bag
Include: Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes；Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after soaked in solvent pulverizing, obtains soak；By ultrasonic for described soak
Process；Product after supersound process is filtered, takes filter liquor；By filter liquor decompression distillation, take concentrated solution；Described concentrated solution is dried
Dry；Wherein, described Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is (5～20) with the weight ratio of described solvent: 1.
In the further embodiment of the present invention, described solvent is water or ethanol solution.
In the further embodiment of the present invention, the mass concentration of described ethanol solution is 55%～65%.
In the further embodiment of the present invention, when described solvent is water, the temperature of described supersound process be 75 DEG C～
80℃。
In the further embodiment of the present invention, when described solvent is ethanol, the temperature of described supersound process is 65 DEG C
～75 DEG C.
In the further embodiment of the present invention, the time of described supersound process is 30 minutes～60 minutes.
In the further embodiment of the present invention, described drying temperature is 65 DEG C～85 DEG C.
In the further embodiment of the present invention, the time of described solvent soaking is 3 hours～5 hours.
In the further embodiment of the present invention, the temperature of described decompression distillation is 50 DEG C～65 DEG C.
The extracting method of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins that the present invention provides, dries Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti, pulverizes；Soaked in solvent powder
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after broken, obtains soak.Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after drying is pulverized, the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after soaked in solvent pulverizing,
The permeability of solvent can be strengthened, also help the more saponin of leaching simultaneously.The soak supersound process that will obtain；Utilize super
The judder that sound produces, high acceleration and strong cavitation effect destroy the plant cell within Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti, make solvent
Penetrate in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti ground product faster, promote the dissolving of saponin in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti further；I.e. utilize ultrasonic extraction,
The extraction ratio of saponin is improved while shortening extraction time.Product after supersound process is filtered, undissolved in molten to remove
Solid impurity in agent.The filter liquor decompression distillation that will be filtrated to get, to reclaim the solvent in leaching process, reduces disappearing of solvent
Consumption；Obtain the saponin of high enrichment simultaneously.Concentrated solution is dried and i.e. can get the total saponins in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti.
Accompanying drawing explanation
Fig. 1 is the flow chart of the extracting method of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins in the embodiment of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, embodiments of the invention are described in detail.Following example are only used for clearer
Explanation technical scheme, be therefore only used as example, and can not limit the scope of the invention with this.
As it is shown in figure 1, the present invention provides the extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins, it is characterised in that including:
S101: Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes.Drying, pulverizing Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti are to obtain the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti of powder, convenient
Follow-up processing procedure.
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after the pulverizing of S102: soaked in solvent, obtains soak.Wherein, described Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is molten with described
The weight ratio of agent is (5～20): 1；Described solvent is water or mass concentration is 55%～65% ethanol solution；Described soak time
It it is 3 hours～5 hours.Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after solvent soaking pulverizing, can strengthen the permeability of solvent, also help leaching simultaneously
More saponin.
S103: by described soak supersound process.Wherein, when described solvent is water, described supersound process be at 75 DEG C～
At 80 DEG C ultrasonic 30 minutes～60 minutes；When described solvent is ethanol, described supersound process is at 65 DEG C～75 DEG C ultrasonic 30
Minute～60 minutes.The soak supersound process that will obtain, utilizes the judder of ultrasonic generation, high acceleration and strong
Cavitation effect destroys the plant cell within Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti, makes solvent penetrate in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti ground product faster, further
Promote the dissolving of saponin in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti；I.e. utilize ultrasonic extraction, while shortening extraction time, improve the extraction of saponin
Rate.When described solvent is water, at 75 DEG C～80 DEG C, carry out supersound process；When described solvent is ethanol, at 65 DEG C～75 DEG C
Carry out supersound process；Under this temperature conditions, saponin extraction rate is higher, extraction time is shorter and will not destroy the structure of saponin.
The structure of saponin it is easily destroyed, so that extraction ratio declines when temperature is the highest；When temperature is the lowest, extraction rate is slow and saponin
Extraction ratio low.
S104: the product after supersound process is filtered, takes filter liquor.Product after supersound process is filtered, to remove not
The solid impurity being dissolved in solvent.
S105: by filter liquor decompression distillation, take concentrated solution.Wherein, decompression distillation is carried out, directly under the conditions of 50 DEG C～65 DEG C
Steaming to no liquid.The filter liquor decompression distillation that will be filtrated to get, decompression distillation, to reclaim the solvent in leaching process, subtracts
The consumption of few solvent；Obtain the saponin of high enrichment simultaneously.
S106: described concentrated solution is dried.Dry at 65 DEG C～85 DEG C that concentrated solution i.e. can get in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is total
Saponin.
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment one
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes；The Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti being soaked in water after pulverizing 3 hours, wherein, the Hylotelephium erythrostictum (Miq.) H.Ohba scape after pulverizing
It is 5:1 with the weight ratio of water, obtains soak；By soak at 75 DEG C ultrasonic 30 minutes；By the product after supersound process
Filter, take filter liquor；Filter liquor is reduced pressure at 50 DEG C distillation, till solvent-free steaming, take concentrated solution；Concentrated solution is existed
The total saponins obtaining in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried at 65 DEG C.
Embodiment two
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes；The Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti being soaked in water after pulverizing 5 hours, wherein, the Hylotelephium erythrostictum (Miq.) H.Ohba scape after pulverizing
It is 20:1 with the weight ratio of water, obtains soak；By soak at 80 DEG C ultrasonic 60 minutes；By the product after supersound process
Filter, take filter liquor；Filter liquor is reduced pressure at 65 DEG C distillation, till solvent-free steaming, take concentrated solution；Concentrated solution is existed
The total saponins obtaining in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried at 75 DEG C.
Embodiment three
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes；The Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti being soaked in water after pulverizing 4 hours, wherein, the Hylotelephium erythrostictum (Miq.) H.Ohba scape after pulverizing
It is 15:1 with the weight ratio of water, obtains soak；By soak at 80 DEG C ultrasonic 50 minutes；By the product after supersound process
Filter, take filter liquor；Filter liquor is reduced pressure at 70 DEG C distillation, till solvent-free steaming, take concentrated solution；Concentrated solution is existed
The total saponins obtaining in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried at 85 DEG C.
Select water as solvent, because the hydrophilic of saponin is relatively strong, water has preferable dissolubility, so selecting water logging
Bubble can leach the saponin in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti to a great extent；And make solvent with water, low cost and having no side effect.When
So, situation about enumerating except embodiment one to embodiment three, other soak times, ultrasonic temperature, ultrasonic time, decompression are distilled
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after temperature, drying temperature and pulverizing is also possible with other ratios of water；Simply in embodiment one to reality
Executing in the case of example three enumerates, the extraction ratio of total saponins is higher.
During it addition, select ethanol to make solvent, the most specifically there are following several embodiments.
Embodiment four
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes；Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after pulverizing by the soak with ethanol that mass concentration is 55% 3 hours,
Wherein, the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after pulverizing is 5:1 with the weight ratio of water, obtains soak；By soak at 65 DEG C ultrasonic 30 minutes；
Product after supersound process is filtered, takes filter liquor；Filter liquor is reduced pressure at 50 DEG C distillation, till solvent-free steaming,
Take concentrated solution；Concentrated solution is dried at 65 DEG C the total saponins obtaining in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti.
Embodiment five
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes；Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after pulverizing by the soak with ethanol that mass concentration is 65% 5 hours,
Wherein, the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after pulverizing is 20:1 with the weight ratio of water, obtains soak；By soak at 75 DEG C ultrasonic 60 points
Clock；Product after supersound process is filtered, takes filter liquor；Filter liquor is reduced pressure at 65 DEG C distillation, until solvent-free steam into
Only, concentrated solution is taken；Concentrated solution is dried at 75 DEG C the total saponins obtaining in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti.
Embodiment six
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes；Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after pulverizing by the soak with ethanol that mass concentration is 60% 4 hours,
Wherein, the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after pulverizing is 15:1 with the weight ratio of water, obtains soak；By soak at 70 DEG C ultrasonic 50 points
Clock；Product after supersound process is filtered, takes filter liquor；Filter liquor is reduced pressure at 70 DEG C distillation, until solvent-free steam into
Only, concentrated solution is taken；Concentrated solution is dried at 85 DEG C the total saponins obtaining in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti.
Certainly, situation about enumerating except embodiment four to embodiment six, other soak times, ultrasonic temperature, ultrasonic time,
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after the temperature of decompression distillation, drying temperature and pulverizing is also possible with other ratios of ethanol；Simply exist
In the case of embodiment four to embodiment six is enumerated, the extraction ratio of total saponins is higher.
It addition, the product after drying for concentrated solution in embodiment one to embodiment six detects, also include following reality
Execute mode:
Embodiment seven
By in embodiment one to embodiment six, the product after concentrated solution drying takes sub-fraction and is respectively placed in 6 small beakers
In, 6 small beakers add a certain amount of water and carries out foaming experiment；Experiment finds, the aqueous solution in 6 small beakers is through strong
Shaking all can produce persistent foam, and foam does not disappears because of heating.
It addition, the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti that the total saponins extraction ratio present invention obtained extracts with microwave loss mechanisms and acetone extraction
Total saponins extraction ratio is made comparisons, and also specifically includes implementation below.
Embodiment eight
The present invention uses the extraction ratio of gravimetric detemination total saponins, concrete detection method to be:
Quality × 100% of the quality of the extraction ratio of total saponins=Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins/Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti powder.Measure and implement
The extraction ratio of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins in example one to embodiment six, and with the Hylotelephium erythrostictum (Miq.) H.Ohba extracted by microwave loss mechanisms and acetone extraction
Herba hylotelephii erythrosticti total saponins extraction ratio is made comparisons, shown in table specific as follows:
Therefore, the extracting method of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins that the present invention provides, simple to operate, shortening the same of extraction time
The extraction ratio of Shi Tigao total saponins.
In describing the invention, it is to be understood that term " first ", " second " are only used for describing purpose, and can not
It is interpreted as instruction or hint relative importance or the implicit quantity indicating indicated technical characteristic.Thus, define " the
One ", the feature of " second " can express or implicitly include one or more this feature.In describing the invention,
" multiple " are meant that two or more, unless otherwise expressly limited specifically.
In the description of this specification, reference term " embodiment ", " some embodiments ", " example ", " specifically show
Example " or the description of " some examples " etc. means to combine this embodiment or example describes specific features, structure, material or spy
Point is contained at least one embodiment or the example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.And, the specific features of description, structure, material or feature can be in office
One or more embodiments or example combine in an appropriate manner.Additionally, in the case of the most conflicting, the skill of this area
The feature of the different embodiments described in this specification or example and different embodiment or example can be tied by art personnel
Close and combination.
Although above it has been shown and described that embodiments of the invention, it is to be understood that above-described embodiment is example
Property, it is impossible to being interpreted as limitation of the present invention, those of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, revises, replaces and modification.
Claims (9)
1. the extracting method of a Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins, it is characterised in that including:
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is dried, pulverizes；
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti after soaked in solvent pulverizing, obtains soak；
By described soak supersound process；
Product after described supersound process is filtered, takes filter liquor；
By the decompression distillation of described filter liquor, take concentrated solution；
Described concentrated solution is dried；
Wherein, described Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is (5～20) with the weight ratio of described solvent: 1.
The extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins the most according to claim 1, it is characterised in that described solvent is water or second
Alcoholic solution.
The extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins the most according to claim 2, it is characterised in that the matter of described ethanol solution
Amount concentration is 55%～65%.
The extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins the most according to claim 2, it is characterised in that when described solvent is water,
The temperature of described supersound process is 75 DEG C～80 DEG C.
The extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins the most according to claim 2, it is characterised in that described solvent is ethanol
Time, the temperature of described supersound process is 65 DEG C～75 DEG C.
The extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins the most according to claim 1, it is characterised in that described supersound process time
Between be 30 minutes～60 minutes.
The extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins the most according to claim 1, it is characterised in that described drying temperature is 65
DEG C～85 DEG C.
The extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins the most according to claim 1, it is characterised in that described solvent soaking time
Between be 3 hours～5 hours.
The extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins the most according to claim 1, it is characterised in that the temperature of described decompression distillation
Degree is 50 DEG C～65 DEG C.
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| CN201610679992.4A CN106265843A (en) | 2016-08-16 | 2016-08-16 | A kind of extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti total saponins |
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| FR3099992A1 (en) * | 2019-08-21 | 2021-02-26 | Laboratoires De Biologie Vegetale Yves Rocher | COSMETIC USE OF A SPECTABILE SEDUM EXTRACT AS A SLIMMING AGENT, AND COMPOSITIONS CONTAINING SUCH EXTRACT |
| CN113214908A (en) * | 2021-04-29 | 2021-08-06 | 浙江吉叶生物科技有限公司 | Environment-friendly detergent and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| FR3099992A1 (en) * | 2019-08-21 | 2021-02-26 | Laboratoires De Biologie Vegetale Yves Rocher | COSMETIC USE OF A SPECTABILE SEDUM EXTRACT AS A SLIMMING AGENT, AND COMPOSITIONS CONTAINING SUCH EXTRACT |
| CN113214908A (en) * | 2021-04-29 | 2021-08-06 | 浙江吉叶生物科技有限公司 | Environment-friendly detergent and preparation method thereof |
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