CN107674066A - A kind of method that Isoschaftoside is extracted from Desmodium styracifolium - Google Patents

A kind of method that Isoschaftoside is extracted from Desmodium styracifolium Download PDF

Info

Publication number
CN107674066A
CN107674066A CN201711044749.6A CN201711044749A CN107674066A CN 107674066 A CN107674066 A CN 107674066A CN 201711044749 A CN201711044749 A CN 201711044749A CN 107674066 A CN107674066 A CN 107674066A
Authority
CN
China
Prior art keywords
extraction
extracted
desmodium styracifolium
isoschaftoside
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711044749.6A
Other languages
Chinese (zh)
Inventor
谢冬养
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guilin Newtel Biological Technology Co Ltd
Original Assignee
Guilin Newtel Biological Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guilin Newtel Biological Technology Co Ltd filed Critical Guilin Newtel Biological Technology Co Ltd
Priority to CN201711044749.6A priority Critical patent/CN107674066A/en
Publication of CN107674066A publication Critical patent/CN107674066A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D407/00Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
    • C07D407/14Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing three or more hetero rings

Abstract

The invention discloses a kind of method that Isoschaftoside is extracted from Desmodium styracifolium, comprise the steps:Comprise the following steps:1) Desmodium styracifolium is cleaned up, dried, crushed with pulverizer, sieved;2) Subcritical Water Extraction:The raw material handled well is placed in extraction kettle, add the hydroxyethyl cellulose equivalent to the parts by weight of raw material 0.001, deionized deoxygenated water is injected in kettle, 150 160 DEG C of extraction temperature, the 10Mpa of pressure 8, the 1.0h of extraction time 0.5, Desmodium styracifolium is extracted, extraction terminates, pressure release, extract flows into collecting tank and is cooled to room temperature, obtains extract;3) purify:The extract that step 2) is obtained concentrates, and carries out column chromatography by macroreticular resin, 55%v/v ethanol elution, obtains eluent, concentrate, dries, obtain finished product.

Description

A kind of method that Isoschaftoside is extracted from Desmodium styracifolium
Technical field
The present invention relates to the extracting method of biological technical field, and in particular to one kind extracts different summer pagoda from Desmodium styracifolium The method of glycosides.
Background technology
Isoschaftoside (Isoschaftoside), chemical formula C26H28O14, molecular weight 564.492, and Schaftoside (Schaftoside) it is isomer, is a kind of flavone compound, can be from Desmodium styracifolium, rabdosia lophanthide, fine yarn line scented tea Extraction obtains in dish.Isoschaftoside has the function that liver protecting, anti-inflammatory, clearing heat and expelling damp.
Desmodium styracifolium (Herba Desmodii styracifolii) is parts of generic medicinal plants, is wide money in legume The ground drying nest of careless [Desmodium styracifolium (Osh.) Merr.], is embodied in《People's Republic of China's medicine Allusion quotation》In versions in 2010.Former plant is suffruticose draft, is distributed in the provinces and regions such as Guangdong, Guangxi, Fujian, Hunan, and Guangdong is medicinal material Main producing region, be mainly used as medicine with its branches and leaves, have clearing heat and expelling damp, it is treating stranguria, row stone function, for urinary tract infections, stone in urinary system, The illnesss such as cholecystolithiasis, ephritis edema.The prescription of lithangiuria illness is cured mainly, much using Desmodium styracifolium as monarch drug in a prescription, its curative effect is quite It is good.Desmodium styracifolium has the hot dampness removing that disappears, Tonglin Paishi effect, contains all multicomponents such as flavonoids, saponins, tannin and alkaloid. Proved by pharmacological research:The general flavone of Desmodium styracifolium possesses the effect that prevention calcinm oxalate calculus is formed and recurred, in addition, also having The effect of significant anti-inflammatory and increase coronary blood flow etc..
Extraction of the prior art to flavones, ultrasonic extraction method Flavonoid substances mainly are used, its principle is ultrasonic Destruction of the cavitation to cell membrane, contributing to the release and dissolution of flavone compound, ultrasonic wave makes extract solution constantly shake, Promote solutes accumulation, concussion effect caused by ultrasonic wave may additionally facilitate the release, diffusion and dissolving of intracellular matter, be advantageous to carry The recovery rate of high extract.The fuel factor of ultrasonic wave makes water temperature at 57 DEG C, there is water-bath effect to raw material substantially simultaneously.Therefore, surpass Sonic method shortens extraction time, improves the extraction rate of active ingredient, the utilization rate of raw material relative to heating extraction.Have big Measure document report and extract Flavonoid substances from the root of kudzu vine, Chinese celery, duck wheat, ginkgo leaf etc..
Existing extracting process, application steam distillation and organic solvent extractionprocess in terms of volatile oil are at present should With widest traditional plant volatile oil or the extracting process of active ingredient, but operating time length, high energy consumption, poor selectivity, have The defects of solvent easily remains is so that the industrialization of both conventional methods, scale application are greatly limited.SFE technologies So that its is nontoxic, residual quantity is low, active ingredient is not easy the advantages that destroyed and enjoys praise.But due to common solvent C02With non-pole Property and relative molecular mass it is small the characteristics of so that it is relatively low to the solubility of Cucumber, selectivity is not high and makes extraction effect simultaneously It is undesirable, often extraction yield is improved by adding entrainer in practical operation, after extraction terminates, it is necessary to try to remove volatilization Entrainer in oil, this certainly will cause the complexity of technique and the loss and oxidation of volatile component;Do not dissolved in additionally, due to water C02, thus overcritical C02Often raw material need to be pre-dried for extraction process, adds additional cost, also make phenolic compound oily Lose.In addition, when volatile oil is extracted from plant, due to overcritical C02Not only to volatile oil but also to the change of low polarity Wax, aliphatic acid, coloring matter and resin in compound such as cuticula also have a similar dissolubility, these materials in extraction process Also can be extracted, it is cumbersome and to equipment although purer volatile oil can also be obtained using complicated system It is it is required that also more harsh.
The critical pressure of water and critical-temperature are respectively 22.1MPa and 374 DEG C, in f>374 DEG C, p>Under the conditions of 22.1MPa, The dielectric constant of water is 5-15.It is referred to as in the low temperature pressure more slightly lower with 22.1MPa than 374.2 DEG C into the water of liquid condition " subcritical water ".Heat water to more than boiling point, below critical point, and control system pressure makes water remain liquid, this shape The water of state is referred to as subcritical water, and it is referred to as superheated water and high-temperature water also to have it in the literature.Under usual conditions, water is polar compounds Thing.Under 505kPa pressure, raise (50-300 DEG C) with temperature, its dielectric constant is decreased to 1 by 70, that is to say, that its property by It is highly polar to fade to nonpolar, solute can be extracted from high to low by polarity.Under conditions of temperature and pressure is all higher, The polarity of water reduces, can be with extracting apolar compound;Under conditions of temperature and pressure is all relatively low, the polarity of water improves, can be with Extract polar compound.During actual extraction, because the influence that influences be not so good as temperature of the pressure to dielectric constant is big, so Mainly the dielectric constant of water is controlled by adjusting temperature.Due to be without using acid, alkali and catalyst water under high thermal high Treatment technology, therefore the extracting method of subcritical water is referred to as " green facture ".In addition, extraction can be in the several seconds Completed in several minutes of short time, so with the advantages of can carrying out continuous processing.Subcritical Water Extraction technology is natural There is very much prospect in the extraction of the active component in product field.
The content of the invention
It is simple with technique it is an object of the invention to provide a kind of method that Isoschaftoside is extracted from Desmodium styracifolium, It is adapted to production, the advantages that time is short.
The present invention is achieved through the following technical solutions.
A kind of method that Isoschaftoside is extracted from Desmodium styracifolium, specifically comprises the following steps:
1) Desmodium styracifolium is cleaned up, dried, crushed with pulverizer, sieved;
2) Subcritical Water Extraction:The raw material handled well is placed in extraction kettle, added equivalent to the parts by weight of raw material 0.001 Hydroxyethyl cellulose, deionized deoxygenated water is injected in kettle, 150-160 DEG C of extraction temperature, pressure 8-10Mpa, extraction time 0.5-1.0h, Desmodium styracifolium is extracted, extraction terminates, pressure release, and extract flows into collecting tank and is cooled to room temperature, is extracted Take liquid;
3) purify:The extract that step 2) is obtained is concentrated, and column chromatography, 55% (v/v) second are carried out by macroreticular resin Alcohol elutes, and obtains eluent, concentrates, and dries, obtains finished product.
Sieving described in step 1) of the present invention, preferably cross 20-80 mesh sieves.
Deionized deoxygenated water is injected in kettle described in step 2), preferably by deionized deoxygenated water with less than 10mL/min's In flow velocity injection kettle, solid-liquid ratio 1:2-3.
Concentration described in step 3), preferable temperature control≤50 DEG C, and vacuum control is concentrated into -0.08 ± 0.01MPa 0.95-1.05g/ml。
Macroreticular resin described in step 3), resin model preferred D101, DM130 and AB-8.
Column chromatography, preferably wet method dress post, sample concentration 8.0mg/mL, loading are carried out by macroreticular resin described in step 3) Measure as 3BV, during elution, be first washed with water closely colourless to efflux, then use 6BV 55% (v/v) ethanol elution, collect elution Liquid.
Compared with prior art, advantages of the present invention:
1st, in the prior art, either ultrasonic extraction method, heating extraction, enzymolysis and extraction etc., ash rate is higher, typically exists 3-5% or so, and many American-European clients also require ash content below 3% while high content is required now, and existing work Skill, traditional technique can not directly produce the product of such specification.And the Isoschaftoside that the method for the present invention obtains, content More than 99.5%, ash content is below 1.5%.
2nd, for the present invention in Subcritical Water Extraction, it is crucial that a certain amount of hydroxyethyl cellulose is added in material.Preceding If the phase it was found that, do not add hydroxyethyl cellulose, directly using Subcritical Water Extraction, obtained final products ash Divide (3% or so) compared to the prior art there is no otherness, and add hydroxyethyl cellulose, then can significantly reduce final production Product ash content (less than 1.5%), analysis are because extraction quantity is with extraction temperature and extraction time during Subcritical Water Extraction Increase and increase, extract the dissolution of product, while active component dissolution, the dissolution of other compositions can also increased, And with the addition of hydroxyethyl cellulose, a certain degree of parcel is formd to the material of extraction, can be in the molten of active component Go out and prevent from reaching a balance between the dissolutions of other impurities.
3rd, the purifying after existing extracting method, the reuse of resin, typically at 3-4 times, the rate of recovery of Isoschaftoside It can decline, cause content to decline, it is necessary to regenerate, and the method for using the present invention, resin may be reused more than 8 times, just need Regeneration, has saved a large amount of man-hours and cost.
Embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1:
A kind of method that Isoschaftoside is extracted from Desmodium styracifolium, specifically comprises the following steps:
1) Desmodium styracifolium is cleaned up, dried, crushed with pulverizer, cross 20 mesh sieves;
2) Subcritical Water Extraction:The raw material handled well is placed in extraction kettle, added equivalent to the parts by weight of raw material 0.001 Hydroxyethyl cellulose, deionized deoxygenated water is injected in kettle with the flow velocity less than 10mL/min, 150-160 DEG C of extraction temperature, pressure Power 8-10Mpa, extraction time 0.5h, is extracted to Desmodium styracifolium, and extraction terminates, pressure release, and extract flows into cold in collecting tank But to room temperature, extract is obtained;
3) purify:The extract that step 2) is obtained concentrates, temperature control≤50 DEG C, vacuum control -0.08 ± 0.01MPa, 0.95-1.05g/ml is concentrated into, column chromatography, sample concentration 8.0mg/mL, loading are carried out by DM130 macroreticular resins Measure as 3BV, during elution, be first washed with water closely colourless to efflux, then use 6BV 55% (v/v) ethanol elution, collect elution Liquid, concentrate, dry, obtain finished product.
Embodiment 2:
A kind of method that Isoschaftoside is extracted from Desmodium styracifolium, specifically comprises the following steps:
1) Desmodium styracifolium is cleaned up, dried, crushed with pulverizer, cross 40 mesh sieves;
2) Subcritical Water Extraction:The raw material handled well is placed in extraction kettle, added equivalent to the parts by weight of raw material 0.001 Hydroxyethyl cellulose, deionized deoxygenated water is injected in kettle with the flow velocity less than 10mL/min, 150-160 DEG C of extraction temperature, pressure Power 8-10Mpa, extraction time 1.0h, is extracted to Desmodium styracifolium, and extraction terminates, pressure release, and extract flows into cold in collecting tank But to room temperature, extract is obtained;
3) purify:The extract that step 2) is obtained concentrates, temperature control≤50 DEG C, vacuum control -0.08 ± 0.01MPa, 0.95-1.05g/ml is concentrated into, column chromatography, sample concentration 8.0mg/mL, loading are carried out by AB-8 macroreticular resins Measure as 3BV, during elution, be first washed with water closely colourless to efflux, then use 6BV 55% (v/v) ethanol elution, collect elution Liquid, concentrate, dry, obtain finished product.
Embodiment 3:
A kind of method that Isoschaftoside is extracted from Desmodium styracifolium, specifically comprises the following steps:
1) Desmodium styracifolium is cleaned up, dried, crushed with pulverizer, cross 80 mesh sieves;
2) Subcritical Water Extraction:The raw material handled well is placed in extraction kettle, added equivalent to the parts by weight of raw material 0.001 Hydroxyethyl cellulose, deionized deoxygenated water is injected in kettle with the flow velocity less than 10mL/min, 150-160 DEG C of extraction temperature, pressure Power 8-10Mpa, extraction time 1.0h, is extracted to Desmodium styracifolium, and extraction terminates, pressure release, and extract flows into cold in collecting tank But to room temperature, extract is obtained;
3) purify:The extract that step 2) is obtained concentrates, temperature control≤50 DEG C, vacuum control -0.08 ± 0.01MPa, 0.95-1.05g/ml is concentrated into, column chromatography, sample concentration 8.0mg/mL, loading are carried out by D101 macroreticular resins Measure as 3BV, during elution, be first washed with water closely colourless to efflux, then use 6BV 55% (v/v) ethanol elution, collect elution Liquid, concentrate, dry, obtain finished product.
In early stage, by contrast experiment, effect is carried out to Different Extraction Method and has compared (the same embodiment of following purification steps 3):
Comparative example 1:
The preparation of water extraction extract solution:Weigh dry Desmodium styracifolium coarse powder (crossing 20 mesh sieves) 10g, water 10mL, immersion 30min, boiling rear slow fire and decoct 40min slowly, filtered while hot with 4 layers of gauze, the dregs of a decoction add water 100mL, decoct 30min after boiling slowly again, Filter while hot, merge decocting liquid twice, be concentrated to 20mL, 55% methanol dissolves and is settled to 50mL.
Comparative example 2:
The preparation of alcohol extracting method extract solution:Dry Desmodium styracifolium coarse powder (crossing 20 mesh sieves) 10g is weighed, adds 60% ethanol 100mL, Backflow twice, first time 2h, second of 1.5h, merges extract solution twice, is concentrated to 20mL, 55% methanol dissolves and is settled to 50mL。
Comparative example 3:
The preparation of enzymatic isolation method extract solution:Dry Desmodium styracifolium coarse powder (crossing 20 mesh sieves) 10g is weighed, adds 10 times of amount water to mix, Cellulase 006g, enzymolysis 1h (temperature 50 C, pH 6) are added, then with 60% ethanol solution relative to 6 times of amounts of coarse powder 1h, cooling filtration are extracted, the dregs of a decoction add 5 times of 60% alcohol reflux 30min of amount, cooling filtration, discard the dregs of a decoction, merge extract solution, dense 20mL is reduced to, 55% methanol dissolves and is settled to 50mL.
Comparative example 4:
The preparation of ultrasonic method extract solution:Coarse powder is ground into, weighs 30.0g, is put into 1000mL flask, adds 60% Ethanol, it is put into ultrasonic machine and after ultrasonic extraction 30min at a temperature of 50 DEG C, stops ultrasound, filtering, merge extract solution, recovery Solvent.
Experimental example
1st, Isoschaftoside content assaying method (HPLC)
Chromatographic condition C18Chromatographic column (4.6mm × 250mm, 5 μm);Mobile phase is acetonitrile -0.025mol/L phosphoric acid solutions (three Ethamine adjusts pH to 3.02) (15: 85);30 DEG C of column temperature;Flow velocity 0.9mL/min;Detection wavelength 270nm, the μ L of sample size 10.
As a result:
2nd, resin stability is investigated:The D101 type dried resin lOg of pretreatment are weighed, wet method is fitted into chromatographic column, with 20mg/ ML loading 10mL, after 60mL water washings, with 55% ethanol elution to colourless, collection 8-48mL section eluents, then with largely Water rinses pillar to without alcohol taste, and this process is 1 cycle.This process is repeated, collects the eluent of each collection, and is concentrated, is done It is dry, weigh, calculate content.
Resin measures Isoschaftoside content (%) after being reused many times
When resin is reused 4 times, the Isoschaftoside content of embodiment 3 have dropped 3.2%, resin compared to for the first time When reusing 8 times, the Isoschaftoside content of embodiment 3 have dropped 5.1%, far better than comparative example compared to for the first time.
3rd, in the experiment of early stage, in Subcritical Water Extraction, applicant also added in material a certain amount of xanthans, CMC, but effect and unobvious, compared with the technique without addition, effect does not have otherness.

Claims (6)

  1. A kind of 1. method that Isoschaftoside is extracted from Desmodium styracifolium, it is characterised in that comprise the following steps:
    1) Desmodium styracifolium is cleaned up, dried, crushed with pulverizer, sieved;
    2) Subcritical Water Extraction:The raw material handled well is placed in extraction kettle, adds the hydroxyl second equivalent to the parts by weight of raw material 0.001 Base cellulose, deionized deoxygenated water is injected in kettle, 150-160 DEG C of extraction temperature, pressure 8-10Mpa, extraction time 0.5- 1.0h, Desmodium styracifolium is extracted, extraction terminates, pressure release, and extract flows into collecting tank and is cooled to room temperature, is extracted Liquid;
    3) purify:The extract that step 2) is obtained is concentrated, and column chromatography is carried out by macroreticular resin, 55%v/v ethanol elution, Eluent is obtained, is concentrated, dries, obtains finished product.
  2. A kind of 2. method that Isoschaftoside is extracted from Desmodium styracifolium according to claim 1, it is characterised in that:Step 1) sieving described in, it was 20-80 mesh sieves.
  3. A kind of 3. method that Isoschaftoside is extracted from Desmodium styracifolium according to claim 1, it is characterised in that:Step 2) injecting deionized deoxygenated water in kettle described in, it is with the flow velocity injection kettle less than 10mL/min by deionized deoxygenated water.
  4. A kind of 4. method that Isoschaftoside is extracted from Desmodium styracifolium according to claim 1, it is characterised in that:Step 3) concentration described in, temperature control≤50 DEG C, vacuum control are concentrated into 0.95-1.05g/ml in -0.08 ± 0.01MPa.
  5. A kind of 5. method that Isoschaftoside is extracted from Desmodium styracifolium according to claim 1, it is characterised in that:Step 3) macroreticular resin described in, resin model are selected from D101, DM130 and AB-8.
  6. A kind of 6. method that Isoschaftoside is extracted from Desmodium styracifolium according to claim 1, it is characterised in that:Step 3) column chromatography is carried out by macroreticular resin described in, is wet method dress post, sample concentration 8.0mg/mL, applied sample amount 3BV, elutes When, the subsequent 55%v/v ethanol elution of using 6BV closely colourless to efflux, collection eluent is first washed with water.
CN201711044749.6A 2017-10-31 2017-10-31 A kind of method that Isoschaftoside is extracted from Desmodium styracifolium Pending CN107674066A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711044749.6A CN107674066A (en) 2017-10-31 2017-10-31 A kind of method that Isoschaftoside is extracted from Desmodium styracifolium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711044749.6A CN107674066A (en) 2017-10-31 2017-10-31 A kind of method that Isoschaftoside is extracted from Desmodium styracifolium

Publications (1)

Publication Number Publication Date
CN107674066A true CN107674066A (en) 2018-02-09

Family

ID=61141945

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201711044749.6A Pending CN107674066A (en) 2017-10-31 2017-10-31 A kind of method that Isoschaftoside is extracted from Desmodium styracifolium

Country Status (1)

Country Link
CN (1) CN107674066A (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102329312A (en) * 2011-07-26 2012-01-25 苏州宝泽堂医药科技有限公司 Method for purifying schaftoside
CN104926897A (en) * 2015-06-19 2015-09-23 武汉光谷人福生物医药有限公司 Method and system for extracting and separating novel Schaftoside in Desmodium styracifolium
CN107098942A (en) * 2017-05-02 2017-08-29 江苏大学 A kind of method of kaempferia galamga glycosides in Subcritical Water Extraction radish leaves
CN107582611A (en) * 2017-10-31 2018-01-16 桂林纽泰生物科技有限公司 A kind of method that general flavone is extracted from Desmodium styracifolium
CN107652259A (en) * 2017-10-31 2018-02-02 桂林纽泰生物科技有限公司 A kind of method that Quercetin is extracted from Desmodium styracifolium
CN107652179A (en) * 2017-10-31 2018-02-02 桂林纽泰生物科技有限公司 A kind of method that forulic acid is extracted from Desmodium styracifolium
CN107840862A (en) * 2017-10-31 2018-03-27 桂林纽泰生物科技有限公司 A kind of method that isoquercitrin is extracted from Desmodium styracifolium
CN107840844A (en) * 2017-10-31 2018-03-27 桂林纽泰生物科技有限公司 A kind of method that Schaftoside is extracted from Desmodium styracifolium

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102329312A (en) * 2011-07-26 2012-01-25 苏州宝泽堂医药科技有限公司 Method for purifying schaftoside
CN104926897A (en) * 2015-06-19 2015-09-23 武汉光谷人福生物医药有限公司 Method and system for extracting and separating novel Schaftoside in Desmodium styracifolium
CN107098942A (en) * 2017-05-02 2017-08-29 江苏大学 A kind of method of kaempferia galamga glycosides in Subcritical Water Extraction radish leaves
CN107582611A (en) * 2017-10-31 2018-01-16 桂林纽泰生物科技有限公司 A kind of method that general flavone is extracted from Desmodium styracifolium
CN107652259A (en) * 2017-10-31 2018-02-02 桂林纽泰生物科技有限公司 A kind of method that Quercetin is extracted from Desmodium styracifolium
CN107652179A (en) * 2017-10-31 2018-02-02 桂林纽泰生物科技有限公司 A kind of method that forulic acid is extracted from Desmodium styracifolium
CN107840862A (en) * 2017-10-31 2018-03-27 桂林纽泰生物科技有限公司 A kind of method that isoquercitrin is extracted from Desmodium styracifolium
CN107840844A (en) * 2017-10-31 2018-03-27 桂林纽泰生物科技有限公司 A kind of method that Schaftoside is extracted from Desmodium styracifolium

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
张蕾,等: "大孔吸附树脂富集纯化广金钱草总黄酮的工艺研究", 《中草药》 *
朱洪法: "《精细化工常用原材料手册》", 1 December 2003 *
郭娟,等: "亚临界水萃取技术在天然产物提取中的研究进展", 《现代化工》 *
郭盼盼: "广金钱草总黄酮纯化工艺优化及总黄酮提取物的抗氧化活性、定性和定量研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 *

Similar Documents

Publication Publication Date Title
CN100503626C (en) Producing raw material containing benzyl carbinol glycosides from Cistanche deserticola by using membrane separation technique and preparation method thereof
CN103961381B (en) Method for negative-pressure boiling extraction and preparation of low-acid ginkgo extract
CN101817816A (en) Method for preparing silybin
CN107556354A (en) The method that isoquercitrin is extracted from beggar-ticks
CN102532244A (en) Method for preparing high-purity asiaticosid
CN103520233A (en) Preparation method and application of apocynum venetum leaf total polyphenol
CN100439319C (en) Method for preparing salviol acid A
CN104771930B (en) The Subcritical Water Extraction agent of polyphenols and extracting process in a kind of lotus solid waste
CN107556277A (en) Quercetin, the method for isoquercitrin are extracted from hyperici,herba
CN107652258A (en) Quercetin, the method for isoquercitrin are extracted from beggar-ticks
CN107669716A (en) The method that general flavone is extracted from desmodium
CN107582593A (en) The method that general flavone is extracted from beggar-ticks
CN107519220A (en) The extracting method of flavone compound in a kind of sunset abelmoschus flower
CN107556194A (en) The method of chlorogenic acid extracting from desmodium
CN107840844A (en) A kind of method that Schaftoside is extracted from Desmodium styracifolium
CN107006854A (en) A kind of cocoa polyphenol is extracted and enrichment preparation method
CN102579564B (en) Preparing method of Tibetan capillaris effective part extractives
CN107652259A (en) A kind of method that Quercetin is extracted from Desmodium styracifolium
CN107582611A (en) A kind of method that general flavone is extracted from Desmodium styracifolium
CN107840862A (en) A kind of method that isoquercitrin is extracted from Desmodium styracifolium
CN104224867B (en) A kind of desmodium sugar-free particle and preparation method thereof
CN107652179A (en) A kind of method that forulic acid is extracted from Desmodium styracifolium
CN107674103A (en) The method that Hyperoside is extracted from beggar-ticks
CN107674102A (en) The method that rutin is extracted from beggar-ticks
CN107840863A (en) The method that isoquercitrin is extracted from hyperici,herba

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20180209

RJ01 Rejection of invention patent application after publication