CN104491836B - Prevention senile dementia health products containing european bird cherry anthocyanin - Google Patents
Prevention senile dementia health products containing european bird cherry anthocyanin Download PDFInfo
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Abstract
The present invention relates to a kind of prevention senile dementia health products containing european bird cherry anthocyanin.Composition by weight percentage is as follows: european bird cherry anthocyanin 30% 50%, european bird cherry polysaccharide 10% 30%, sorghum rice polypeptide 5% 15%, Chinese chestnut polypeptide 5% 15%, yam extract 5% 10%, tuckahoe extracts 5% 10%, Rhizoma Chuanxiong extract 5% 10%, tuber of dwarf lilyturf extract 5% 10%.Its prevention senile dementia function being had is carried out experiment in vitro and animal experiment study, find that these health products have activity and the function of prevention senile dementia, can substitute for chemical synthetic drug, avoid too much chemical synthetic drug toxic and side effect, make european bird cherry pattern glycoside composition have broader development prospect, and become the emerging composition that can apply in health products.
Description
Technical field
The invention belongs to field of health care products, be specifically related to a kind of guarantor containing european bird cherry anthocyanin with prevention senile dementia function
Strong product.
Background technology
Senile dementia, also known as Alzheimer disease (Alzheimer ' s disease, AD), is with cognitive function disappearance as core
Syndrome, be a kind of nerve degenerative diseases, mainly show as gradual memory power and lose and cognitive disorder.Senile dementia
Morbidity concentrates on the elderly of more than 60 years old, and China is as a country that will enter aging, its old dementia patients
Number have the trend of sustainable growth, long due to this disease course of disease again, make a definite diagnosis inspection complexity, therefore in following a period of time, old
Property dementia diagnosis and treatment will be a major challenge.
At present, research finds, the cause of disease of senile dementia has a lot, relevant with the neure damage in cerebral hippocampus district after all,
Region relevant with memory and cognition in brain in patients produces neuropathy patch, is mainly composed of polypeptide, β-starch in patch
The amyloid that sample peptide (A β) is polymerized and get involved in malnutrition therein or the neural dendron of sex change and aixs cylinder, with
And some inflammatory reaction Deiter's cells.Owing to its pathogenic factors relates to a lot of aspect, such as: acetylcholine precursor thing,
But it is the best to there is clinical trial result, or the problem such as toxic and side effect is bigger.Therefore, senile dementia focuses on prevention, in time
Nutriment required for reasonable supplement brain cell physiology, can strengthen the physiological function of brain, thus prevent sending out of senile dementia
Raw.
Summary of the invention
It is an object of the invention to belong to european bird cherry with the Northeast ornamental plant european bird cherry set out, prepare european bird cherry anthocyanin, european bird cherry polysaccharide,
Mix with other active materials, it is provided that a kind of natural health-care products preventing senile dementia.
Carry with european bird cherry anthocyanin, european bird cherry polysaccharide, sorghum rice polypeptide, Chinese chestnut polypeptide, tuckahoe extracts, yam extract, Ligusticum wallichii
Take thing, tuber of dwarf lilyturf extract is used in mixed way as a kind of natural health-care products preventing senile dementia, and each by weight percentage composition is such as
Under:
European bird cherry anthocyanin 30%-50%, european bird cherry polysaccharide 10%-30%, sorghum rice polypeptide 5%-15%,
Chinese chestnut polypeptide 5%-15%, yam extract 5%-10%, tuckahoe extracts 5%-10%,
Rhizoma Chuanxiong extract 5%-10%, tuber of dwarf lilyturf extract 5%-10%.
The european bird cherry anthocyanin that the present invention relates to is extracted by european bird cherry, uses the work of ultrasonic-microwave-complex enzyme zymohydrolysis method of fractional steps
European bird cherry anthocyanin is extracted in skill combination.European bird cherry polysaccharide is also extracted by european bird cherry, uses ultrasonic-microwave-complex enzyme zymohydrolysis substep
The process combination of method extracts european bird cherry polysaccharide.Sorghum rice polypeptide, Chinese chestnut polypeptide are prepared by enzymolysis sorghum rice albumen, Chinese chestnut albumen.
The process combination using complex enzyme zymohydrolysis-microwave-ultrasonic method of fractional steps is extracted european bird cherry anthocyanin and enters with AB-8 macroreticular resin
The step that row static state purifies is as follows:
1) complex enzyme zymohydrolysis extracts: european bird cherry fruit is gone handle, broken, and stoning puts into subzero 80 DEG C of refrigerator freezings overnight, cold
Lyophilizer is dried, taking-up afterwards to be dried, pulverizes.Solid-liquid ratio 1:10-1:50 in mass ratio, is lyophilized european bird cherry
Powder is dissolved in water, and complex enzyme addition is the 1.0%-5.0% of the gross weight of european bird cherry freeze-dried powder, carries out hot water extraction, controls temperature
Degree is for 30-70 DEG C, and pH value range 3.0-6.0 enzymolysis time is 30min-60min, centrifugal, filters, and extract is standby,
Sediment proceeds Microwave Extraction；What complex enzyme used was cellulase with pectase 1:0.5 in mass ratio～2 is mixed
Close；
2) microwave radiation exaraction: solid-liquid ratio 1:10-1:15 in mass ratio, is dissolved in water, microwave power 300W-by the sediment after enzymolysis
500W, the microwave time is 20min-60min, centrifugal, filters, and extract is standby, and sediment proceeds ultrasonic leaching
Carry；
3) ultrasonic extraction: solid-liquid ratio 1:10-1:15 in mass ratio, is dissolved in the sediment after microwave in ethanol, and ultrasonic power is
100W-500W, ultrasonic time is 10min-50min, and pH value is 2.0-6.0, suction filtration, and extract is standby；
4) merging the extract of complex enzyme zymohydrolysis, the extract of microwave radiation technology and the extract of ultrasonic extraction, extract rotary evaporation is dense
Contracting, obtains thick european bird cherry anthocyanin dry powder after freeze-drying；
5) macroreticular resin static state purifies: thick european bird cherry anthocyanin dry powder is made into the aqueous solution of 8mg/ml, and Static Adsorption condition is: temperature
20 DEG C, the addition of resin is 0.16g/ml；Analysis condition is: strippant 70% ethanol, and pH value is 3.0, and temperature is
60 DEG C, obtain european bird cherry anthocyanin dry powder.
The process combination using ultrasonic-microwave-complex enzyme zymohydrolysis method of fractional steps extracts european bird cherry polysaccharide and by Sephadex G-200 layer
The step that analysis purifies is as follows:
1) ultrasonic extraction: european bird cherry fruit is removed handle, broken, stoning, put into subzero 80 DEG C of refrigerator freezings overnight, in freeze-drying
Machine is dried, taking-up afterwards to be dried, pulverizes.By soluble in water for european bird cherry freeze-dried powder, solid-liquid ratio 1:10-in mass ratio
1:50, controls temperature and is 50-90 DEG C, and pH value 5.0-9.0, ultrasonic power is 100W-500W, and ultrasonic time is
10min-50min, centrifugal, collect extract and proceed Microwave Extraction；
2) microwave radiation exaraction: take extract, microwave power 300W-500W, the microwave time is 20min-60min, centrifugal, receives
Collection extract proceeds complex enzyme zymohydrolysis；
3) complex enzyme zymohydrolysis extracts: take extract, adds the complex enzyme of extract weight 1.0%-5.0%, and control temperature is 30-60
DEG C, pH 3.0-6.0, enzymolysis time is 30min-60min, centrifugal, and suction filtration takes filtrate, and filtrate rotary evaporation concentrates,
Adding 95% ethanol precipitate polysaccharides of 4 times of volumes, remove supernatant after 3000r/min is centrifugal, pellet frozen is dried,
European bird cherry Thick many candies.Described complex enzyme is the mixing of cellulase and pectase 1:0.5 in mass ratio～2；
4) again being dissolved by the cryodesiccated european bird cherry Thick many candies appropriate distilled water of addition, Sephadex G-200 chromatography carries out separating pure
Changing, elute with distilled water, flow velocity is 1ml/min, and every 2ml collects a test tube, with H2SO4-anthrone method tracing detection extremely
Sugar-free, collects the most polysaccharide component of content and dialyses in distilled water 24h, and freeze-drying carries out subsequent experimental.With
DEAE-32 is further purified, and phosphate buffer-water is as flowing phase, and gradient mode elutes, and flow velocity is 0.8
Ml/min, every 2ml collect a test tube, with H2SO4-anthrone method tracing detection to sugar-free, collect the polysaccharide group that content is most
Point.
It is european bird cherry anthocyanin, european bird cherry polysaccharide, sorghum rice polypeptide, Chinese chestnut polypeptide, Poria cocos to be extracted that the present invention has the technology of initiative
Thing, yam extract, Rhizoma Chuanxiong extract and tuber of dwarf lilyturf extract are as an overall composition prevention senile dementia, according to existing
, science combination application reasonably combined for nutrition model, is formed and has notable prevention senile dementia mixed health product.
Accompanying drawing explanation
The composition of Fig. 1 embodiment 1 is to H2O2Induction N2The fluorescence micrograph of A cellular damage protection；
Wherein, A: normal group；B: model group；C: compositions-treated 1 group；D: compositions-treated 2 groups.
The composition of Fig. 2 embodiment 1 is to H2O2Induction N2A cellular damage is protected；
Wherein, A: normal group；B: model group；C: compositions-treated 1 group；D: compositions-treated 2 groups.
The composition of Fig. 3 embodiment 1 is to H2O2The N of damage2The fluorescence micrograph of the impact of A reactive oxygen species；
Wherein, A: Normal neuronal cells；B:0.5mmol/l H2O2Process nerve cell；C:0.05mg/ml compositions-treated god
Through cell.
Detailed description of the invention
The embodiment 1 prevention senile dementia health products containing european bird cherry anthocyanin
(1) preparation of european bird cherry anthocyanin
The present embodiment uses the process combination of ultrasonic-microwave-mixed enzyme enzymolysis method of fractional steps extract european bird cherry anthocyanin and use AB-8
Macroreticular resin carries out static purifying, and step is as follows:
1) complex enzyme zymohydrolysis extracts: european bird cherry fruit is gone handle, broken, and stoning puts into subzero 80 DEG C of refrigerator freezings overnight, cold
Lyophilizer is dried, taking-up afterwards to be dried, pulverizes.European bird cherry freeze-dried powder is dissolved in water, and solid-liquid ratio is in mass ratio
1:30, complex enzyme used is the mixing of cellulase and pectase 1:1 in mass ratio, and complex enzyme addition is that european bird cherry is lyophilized
The 3.0% of the gross weight of powder, carries out hot water extraction, and controlling temperature is 50 DEG C, pH value be 5.0 enzymolysis times be 50min,
6000r/min is centrifuged 10min, filters, and extract is standby, and sediment proceeds Microwave Extraction；
2) microwave radiation exaraction: solid-liquid ratio 1:10 in mass ratio, by soluble in water for the sediment after enzymolysis, microwave power 400W,
The microwave time is that 40min, 6000r/min are centrifuged 10min, filters, and extract is standby, and sediment proceeds to surpass
Sound extracts；
3) ultrasonic extraction: solid-liquid ratio 1:10 in mass ratio, is dissolved in the sediment after microwave in 60% ethanol, and ultrasonic power is
400W, ultrasonic time is 30min, and pH value is 4.0, suction filtration, and extract is standby；
4) merging the extract of complex enzyme zymohydrolysis, the extract of microwave radiation technology and the extract of ultrasonic extraction, extract rotary evaporation is dense
Contracting, obtains thick european bird cherry anthocyanin dry powder after freeze-drying；
5) macroreticular resin static state purifies: thick european bird cherry anthocyanin dry powder is made into the aqueous solution of 8mg/ml, and Static Adsorption condition is: temperature
20 DEG C, the addition of resin is 0.16g/ml；Analysis condition is: strippant 70% ethanol, and pH value is 3.0, and temperature is
60 DEG C, obtain european bird cherry anthocyanin dry powder.
(2) preparation of european bird cherry polysaccharide
The process combination using ultrasonic-microwave-complex enzyme zymohydrolysis method of fractional steps extracts european bird cherry polysaccharide and by Sephadex G-200 layer
The step that analysis purifies is as follows:
1) ultrasonic extraction: european bird cherry fruit is removed handle, broken, stoning, put into subzero 80 DEG C of refrigerator freezings overnight, in freeze-drying
Machine is dried, taking-up afterwards to be dried, pulverizes.By soluble in water for european bird cherry freeze-dried powder, solid-liquid ratio is in mass ratio
1:30, controlling temperature is 60 DEG C, pH value 7.0, and ultrasonic power is 400W, and ultrasonic time is 40min,
3000r/min is centrifuged 15min, collects extract and proceeds Microwave Extraction；
2) microwave radiation exaraction: take ultrasonic after extract, microwave power 400W, the microwave time is that 40min, 3000r/min enter
The centrifugal 15min of row, collects extract and proceeds complex enzyme zymohydrolysis；
3) complex enzyme zymohydrolysis extracts: take the extract after microwave, adds the complex enzyme of its gross weight 4.0%, and controlling temperature is 50 DEG C,
PH 4.0, enzymolysis time is that 40min, 3000r/min are centrifuged 15min, suction filtration, and filtrate rotary evaporation concentrates, adds
Enter 95% ethanol precipitate polysaccharides of 4 times of volumes, remove supernatant, freeze-drying after 3000r/min is centrifugal, obtain european bird cherry the most
Sugar.Described complex enzyme is the mixing of cellulase and pectase 1:1 in mass ratio；
4) again being dissolved by the cryodesiccated european bird cherry Thick many candies appropriate distilled water of addition, Sephadex G-200 chromatography carries out separating pure
Changing, elute with distilled water, flow velocity is 1ml/min, and every 2ml collects a test tube, with H2SO4-anthrone method tracing detection extremely
Sugar-free, collects the most polysaccharide component of content and dialyses in distilled water 24h, and freeze-drying carries out subsequent experimental.With
DEAE-32 is further purified, and phosphate buffer-water is as flowing phase, and gradient mode elutes, and flow velocity is 0.8
Ml/min, every 2ml collect a test tube, with H2SO4-anthrone method tracing detection to sugar-free, collect the polysaccharide group that content is most
Point.
(3) preparation of sorghum rice polypeptide
It is as follows that papain extracts sorghum rice polypeptide step:
1) being pulverized by sorghum rice, be 1:15 by solid-liquid ratio, soak 3h in the sodium hydroxide solution that pH is 10.0, Extracting temperature is
50 DEG C, 5000r/min is centrifuged 20min, takes supernatant, precipitates with 90% ammonium sulfate, is again centrifuged, isolated
Precipitation, carries out freeze-drying and obtains sorghum rice albumen；
2) sorghum rice albumen is made into the aqueous solution of 8%, and pH is adjusted to 6.5, and enzyme addition alive is 6000u/g material, and temperature is 60
DEG C, extraction time is 3h, and then boiling water bath 10min goes out enzyme, and 3500r/min is centrifuged 15min, takes supernatant, and pure water is saturating
Analysis, to use molecular cut off be to carry out freeze-drying after l0kDa ultrafiltration plate ultrafiltration, obtains sorghum rice polypeptide powder.
(4) preparation of Chinese chestnut polypeptide
1) Chinese chestnut peeling is dried, pulverizes with pulverizer, is 1:10 by solid-liquid ratio, adds the NaOH of pH 11, and Extracting temperature is
50 DEG C, the time is 3h, centrifugal collecting precipitation, and Chinese chestnut albumen is prepared in freeze-drying.
2) complex enzyme fractional hydrolysis: first, hydrolyzes with compound fertilizer production, the most again with hydrolysis by novo, prepares Chinese chestnut
Polypeptide, compound fertilizer production enzymatic hydrolysis condition is: pH 6.0, and enzyme addition alive is 6000u/g material, time 1h, temperature
55 DEG C, alkali protease enzymatic hydrolysis condition is: pH 8.0, and enzyme addition alive is 6000u/g material, time 3h, temperature 50
℃；Hydrolyzate is heated to 100 DEG C, keeps the vigor killing enzyme for 10 minutes；Using molecular cut off is l0kDa ultrafiltration
The enzymolysis liquid that plate ultrafiltration obtains, collecting molecular weight is the enzymolysis liquid of below 10kDa；By enzymolysis liquid low-temperature freeze-drying machine
Prepare Chinese chestnut polypeptide powder.
(5) preparation of yam extract
At ambient temperature, weighing is ground into the Chinese yam pressed powder of 200 mesh；The ratio of 1:10 in mass ratio weighs Chinese yam solid
Powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate is aseptic cold
Lyophilized dry, obtain yam extract.
(6) preparation of tuckahoe extracts
At ambient temperature, weighing is ground into the Poria cocos pressed powder of 200 mesh；The ratio of 1:10 in mass ratio weighs Poria cocos solid
Powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate is aseptic cold
Lyophilized dry, obtain tuckahoe extracts.
(7) preparation of Rhizoma Chuanxiong extract
At ambient temperature, weighing is ground into the Ligusticum wallichii pressed powder of 200 mesh；The ratio of 1:10 in mass ratio weighs Ligusticum wallichii solid
Powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate is aseptic cold
Lyophilized dry, obtain Rhizoma Chuanxiong extract.
(8) preparation of tuber of dwarf lilyturf extract
At ambient temperature, weighing is ground into the tuber of dwarf lilyturf pressed powder of 200 mesh；The ratio of 1:10 in mass ratio weighs the tuber of dwarf lilyturf
Pressed powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium freeze-drying, get Mai Dongmen extract.
(9) the prevention senile dementia Halth-care composition containing european bird cherry anthocyanin
It is made up of following raw materials by weight:
(10) test cell line
1, composition is to H2O2Induction N2A cellular damage is protected
It is made into single N with obtaining nutrient solution containing 10% hyclone2A cell suspension, with every hole 104Individual cell is inoculated into 6 holes
Plate, every pore volume 2mL, then at 37 DEG C, 5%CO2Incubator is carried out cell cultivate 24h, then to cell at
Reason, arranges 3 experimental group, respectively Normal group, model group and compositions-treated group.Wherein model group H2O2Concentration
For 0.5mmol/l, compositions-treated 1 group is 0.5mmol/l H2O2With the composition of 0.025mg/ml, compositions-treated 2 groups
For 0.5mmol/l H2O2With the composition of 0.05mg/ml, continue to cultivate 1h, under microscope, observe the shape of difference group cell respectively
State.
By the H of 0.5mmol/L2O2Process can significantly reduce N2The vigor of A cell.Add various dose composition it
After, composition can reduce H2O2To N2The damage of A cell, to N2The protected effect of A cell.Its result such as Fig. 1 and Tu
Shown in 2.
From Fig. 1, Fig. 2 it can be seen that normal cell refractivity is strong, cell rule, adhered state is good；H2O2Group cell
Becoming round, refractivity dies down, adherent bad, and compositions-treated group cellular morphology relatively H2O2Group makes moderate progress.H2O2Can damage
Hindering the integrality of cell membrane, reduce the activity of cell, the damage to cell is very big, works as H2O2When concentration is 0.5mmol/l,
Great-hearted cell number only reaches original 52.7%, but, add 0.025mg/ml composition after, great-hearted carefully
Born of the same parents' number is original 71.4%, and when adding the composition of 0.05mg/ml, great-hearted cell number is 91.8%.By
This, it can be deduced that: the composition of various dose is to H2O2The N of induction2A cellular damage has the strongest protective effect, it is possible to
Significantly improve the activity of cell, and there is preferable dose-effect relationship.
2, composition is to H2O2The N of damage2The impact of A reactive oxygen species
By N2A cell carries out kind of a plate, adds composition and the H of 0.5mg/ml2O2Stimulate cell, remove cell culture fluid,
After adding PBS drip washing, add the DCFH-DA diluted of final concentration of 20 μm mol/L, wrap tinfoil, 37 DEG C of cultivations
Incubation 30min in case, is carrying out drip washing with PBS, is adding methyl alcohol 0.5ml ,-20 DEG C of fixing 30min, again after drip washing, add
Entering acetone (4 DEG C of precoolings) 0.5ml and fix 6min, then every 5min is once with PBS drip washing 3 times, finally carries out mounting sight
Examining, result is as shown in Figure 3.
By fluorescence micrograph Fig. 3, in A, normal group cell fluorescence is the dimest, H in B2O2Process N2A cell
Fluorescence Increasing, illustrates that reactive oxygen species level is higher.And after adding composition, the fluorescence intensity in C substantially reduces, with
The degree that normal group cell fluorescence intensity is close, reactive oxygen species level reduce, illustrate composition have strong reduction by
H2O2The effect of the content of the intracellular ROS caused.
(11) zoopery
The composition intervention to mouse cognitive ability.Animal is chosen: kind: ICR rat, sex: male, body weight 18-
22g, quantity: 50.
Packet is tested, and mouse is randomly divided into Normal group (equal-volume physiological saline), model group (equal-volume physiology salt
Water), mixture low, medium and high dosage group (respectively gavage give 50,100,200mg kg-1Composition, every day one
Secondary).Often group 10.In addition to Normal group, each treated animal lumbar injection every day D-galactolipin 150mg/kg and natrium nitrosum
80mg/kg modeling, and give each treated animal gavage relative medicine, continuous 42d.
Morris water maze laboratory: water maze composition is to paint dimmed cylindrical pond and a metal platform.Pond is high
50cm, diameter 80cm, platform diameter 10.0cm.Experiment is carried out in the quiet room of sound insulation, and the laboratory such as pond, mouse cage is each
The position planting object keeps constant.Dying black with black ink after adding water makes water opaque, and labyrinth is divided into 4 quadrants, water
Temperature is maintained at about 25 DEG C.Separately there is a black circular platform (diameter 10cm, high 28cm), be placed in certain quadrant
Centre, is positioned at underwater 1～about 2cm.During test, select quadrant as place of entry, mouse put in water towards pool wall,
Follow the tracks of system record mouse according to water maze and find and climb up platform required time, i.e. escape latency (Escape latency),
Not finding platform that mouse then causes platform in 120s, and stop 20s on platform, escape latency is designated as 120s.It is administered
16th day starts water maze orientation navigation experiment, and every mouse trains 2 times (including 2 quadrant place of entry), time limit every day
It is set to each 120s, platform time of staying 20s, records the escape latency of each group of mouse.Orientation navigation experiment the 6th day
Testing for space exploration, remove platform, mouse is put in water towards pool wall by diagonal quadrant starting point, and record mouse is in 60s
The target quadrant swim time.
Result of the test is as follows:
The impact preclinical on dementia mice platform of table 1 composition
From table 1 result, comparing with Normal group, model control group latency increases substantially；Give height, in,
After the composition of low dosage, low dose group (50mg kg-1Composition) dementia mice have reduction trend, middle dosage incubation period
Group (100mg kg-1Composition) and high dose group (200mg kg-1Composition) dementia mice substantially reduce incubation period.With
Blank group compares, and model group mouse significantly shortens at the swimming time of target quadrant；Compare with model group, in, low dose
The composition group mouse of amount significantly extends at the swimming time of target quadrant.Illustrate that the composition of high, medium and low dosage can improve silly
The ability of learning and memory of slow-witted mouse.
Visible according to result of the test, the present invention contains the prevention senile dementia health products work protected to nerve cell of european bird cherry anthocyanin
With, can improve cognitive ability and ability of learning and memory, thus show prevention senile dementia effect, and have obvious dosage-
Effect relation.Therefore, the present invention has prevention senile dementia effect.
The embodiment 2 prevention senile dementia health products containing european bird cherry anthocyanin
It is made up of following raw materials by weight:
The embodiment 3 prevention senile dementia health products containing european bird cherry anthocyanin
It is made up of following raw materials by weight:
Claims (7)
1. contain the prevention senile dementia health products of european bird cherry anthocyanin, it is characterised in that be made up of following raw materials by weight:
European bird cherry anthocyanin 30%-50%, european bird cherry polysaccharide 10%-30%, sorghum rice polypeptide 5%-15%,
Chinese chestnut polypeptide 5%-15%, yam extract 5%-10%, tuckahoe extracts 5%-10%,
Rhizoma Chuanxiong extract 5%-10%, tuber of dwarf lilyturf extract 5%-10%.
2. the prevention senile dementia health products containing european bird cherry anthocyanin as claimed in claim 1, it is characterised in that: european bird cherry anthocyanin
Preparation method as follows:
1) complex enzyme zymohydrolysis extracts: solid-liquid ratio 1:10-1:50 in mass ratio, and european bird cherry freeze-dried powder is dissolved in water, complex enzyme addition
For the 1.0%-5.0% of the gross weight of european bird cherry freeze-dried powder, carry out hot water extraction, control temperature and be 30-70 DEG C, pH model
Enclosing value 3.0-6.0, enzymolysis time is 30min-60min, centrifugal, filters, and extract is standby, sediment continue into
Row Microwave Extraction；Described complex enzyme is the mixing of cellulase and pectase 1:0.5 in mass ratio～2；
2) microwave radiation exaraction: solid-liquid ratio 1:10-1:15 in mass ratio, is dissolved in water, microwave power 300W-by sediment
500W, the microwave time is 20min-60min, centrifugal, filters, and extract is standby, and sediment proceeds ultrasonic
Extraction；
3) ultrasonic extraction: solid-liquid ratio 1:10-1:15 in mass ratio, is dissolved in sediment in ethanol, and ultrasonic power is 100W-
500W, ultrasonic time is 10min-50min, and pH value is 2.0-6.0, suction filtration, and extract is standby；
4) merging the extract of complex enzyme zymohydrolysis, the extract of microwave radiation technology and the extract of ultrasonic extraction, extract rotates and steams
Send out and concentrate, after freeze-drying, obtain thick european bird cherry anthocyanin dry powder；
5) macroreticular resin static state purifies: thick european bird cherry anthocyanin dry powder is made into the aqueous solution of 8mg/ml, and Static Adsorption condition is: temperature
Spending 20 DEG C, the addition of resin is 0.16g/ml；Analysis condition is: strippant 70% ethanol, and pH value is 3, temperature
Degree is 60 DEG C, obtains european bird cherry anthocyanin dry powder.
3. the prevention senile dementia health products containing european bird cherry anthocyanin as claimed in claim 1, it is characterised in that: described european bird cherry
The preparation method of polysaccharide is as follows:
1) ultrasonic extraction: solid-liquid ratio 1:10-1:50 in mass ratio, by soluble in water for european bird cherry freeze-dried powder, control temperature is 50-
90 DEG C, pH value 5.0-9.0, ultrasonic power is 100W-500W, and ultrasonic time is 10min-50min, from
The heart, takes extract and proceeds Microwave Extraction；
2) microwave radiation exaraction: take extract, microwave power 300W-500W, the microwave time is 20min-60min, from
The heart, takes extract and proceeds complex enzyme zymohydrolysis；
3) complex enzyme zymohydrolysis extracts: taking extract, complex enzyme addition is the 1.0%-5.0% of extract weight, controls temperature
For 30-60 DEG C, pH 3.0-6.0, enzymolysis time is 30min-60min；Suction filtration, filtrate rotary evaporation concentrates, and adds
95% ethanol precipitate polysaccharides of 4 times of volumes, centrifugal, remove supernatant, freeze-drying；Described complex enzyme is fine
Dimension element enzyme and the mixing of pectase 1:0.5 in mass ratio～2.
4. the prevention senile dementia health products containing european bird cherry anthocyanin as claimed in claim 1, it is characterised in that: described Chinese yam
The preparation method of extract: at ambient temperature, weighs the Chinese yam pressed powder being ground into 200 mesh；1:10 in mass ratio
Ratio weighs Chinese yam pressed powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, ultrafiltration
Film ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains yam extract.
5. the prevention senile dementia health products containing european bird cherry anthocyanin as claimed in claim 1, it is characterised in that: described Poria cocos
The preparation method of extract: at ambient temperature, weighs the Poria cocos pressed powder being ground into 200 mesh；1:10 in mass ratio
Ratio weighs Poria cocos pressed powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, ultrafiltration
Film ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains tuckahoe extracts.
6. the prevention senile dementia health products containing european bird cherry anthocyanin as claimed in claim 1, it is characterised in that: described Ligusticum wallichii
The preparation method of extract: at ambient temperature, weighs the Ligusticum wallichii pressed powder being ground into 200 mesh；1:10 in mass ratio
Ratio weighs Ligusticum wallichii pressed powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, ultrafiltration
Film ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains Rhizoma Chuanxiong extract.
7. the prevention senile dementia health products containing european bird cherry anthocyanin as claimed in claim 1, it is characterised in that: described Mai Men
The preparation method of winter extract: at ambient temperature, weighs the tuber of dwarf lilyturf pressed powder being ground into 200 mesh；In mass ratio
1:10 ratio weighs tuber of dwarf lilyturf pressed powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper just mistake
Filter, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains tuber of dwarf lilyturf extract.
Priority Applications (1)
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