CN104906162A - Preparing method and application of rhizoma atractylodis volatile oil - Google Patents
Preparing method and application of rhizoma atractylodis volatile oil Download PDFInfo
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- CN104906162A CN104906162A CN201510263888.2A CN201510263888A CN104906162A CN 104906162 A CN104906162 A CN 104906162A CN 201510263888 A CN201510263888 A CN 201510263888A CN 104906162 A CN104906162 A CN 104906162A
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Abstract
The invention discloses a preparing method and application of rhizoma atractylodis volatile oil, and particularly relates to a method for extracting volatile oil through the reduced pressure distillation method with the assistance of the biological enzymolysis technology. The preparing method comprises the technical steps of raw material processing, immersing, enzymolysis, distillation, extraction and drying, and then the rhizoma atractylodis volatile oil can be obtained. The technical process is simple and reasonable, the operation time is short, the extraction rate of the volatile oil is high, the application range is wide, and very high practical value is achieved. The rhizoma atractylodis volatile oil is especially suitable for the application in preparing anti-tumor products including drugs and healthcare products.
Description
Technical field
The present invention relates to a kind of preparation method of Rhzoma Atractylodis Lanceae volatile oil and gained Rhzoma Atractylodis Lanceae volatile oil preparing the application in antitumor product, belonging to medical art.
Background technology
Cancer is the general designation of a large class malignant tumor.The feature of cancerous cell be unrestrictedly, hypertrophy without end, the nutrient substance in patient body is consumed in a large number; Cancerous cell discharges multiple toxin, makes human body produce series of symptoms; Cancerous cell also can be transferred to whole body growth and breeding everywhere, cause human body to be become thin, unable, anemia, inappetence, heating and serious organ function impaired etc.On the other side have benign tumor, benign tumor is then easily removed clean, generally do not shift, do not recur, extruding and blocking action are only had to organ, tissue, but cancer (malignant tumor) also can disorganize, organ 26S Proteasome Structure and Function, cause downright bad hemorrhage concurrent infection, patient is finally dead due to organ failure.Arrived today of science high speed development, we have reason to believe that cancer is not incurable disease.
The life of the mankind in cancer serious threat, is one of underlying cause of death of most countries in the world today.According to World Health Organization's statistics, the patient about 5,000,000 of cancer is died from the whole world every year, therefore the treatment of cancer and preventative task very arduous.
The dry rhizome that Rhizoma Atractylodis (formal name used at school: Atractylodes lancea) are Atractylodes lancea (Thunb.) DC. or Atractylis chinensis, is generally wild, perennial vertical herbage, the flat sleeping or oblique ascension of root stock, thick long or usually in pimple shape, the raw slightly isometric or closely isometric adventitious root such as most.Stem is upright, and single raw or minority stem becomes to cluster, and bottom or middle part be normal aubergine below, not branch or top but rare from bottom branch, and whole stem branch is by sparse arachnoid hair or without hair.Call red art, Atractylodes spp, Ma Ji, blue or green art, magic arts, Rhizoma Atractylodis Macrocephalae.
The chief value of Rhizoma Atractylodis: have drying damp and strengthening spleen, effect of wind-damp dispelling, cures mainly retention of dampness in middle-JIAO, anemofrigid-damp arthralgia, foot swelling and pain in knee, flaccidity is soft unable, nyctalopia nyctalopia, adjustment gastrointestinal motility function, there is stronger antiulcer action, hepatoprotective effect, Rhizoma Atractylodis extract has the effect eliminating drug resistance shigella flexneri R plasmid, can reduce the generation of bacterial drug resistance.
Other pharmacological actions: (1) has hypoglycemic activity; (2) anti-hypoxia, the main active of Rhizoma Atractylodis anti-hypoxia is sagittol; (3) Atractylodes lancea (Thunb.) DC., Atractylis chinensis, sagittol, hinesol have central inhibitory action; (4) Rhzoma Atractylodis Lanceae volatile oil, hinesol, sagittol have inhibitory action to tumor in vitro, and wherein hinesol effect is stronger; (5) skeleton calcification is promoted, containing the vitamin D relevant with Calcium and phosphorous absorption in Rhizoma Atractylodis.
Rhizoma Atractylodis are seldom seen preparing the application in antitumor product as a kind of Chinese crude drug, thus make the range of application of Rhizoma Atractylodis receive certain restriction, in order to expand the application kind of antitumor drug, the present invention specially provides Rhizoma Atractylodis and is preparing the application in antitumor product.
Summary of the invention
In order to make full use of the nutritive value of Rhizoma Atractylodis, expand the application kind of antitumor product, the preparation method that invention especially provides a kind of Rhzoma Atractylodis Lanceae volatile oil is preparing the application in antitumor product with it.
For achieving the above object, the preparation method of Rhzoma Atractylodis Lanceae volatile oil is by the following technical solutions:
(1) fresh Rhizoma Atractylodis are cleaned up, remove root, be cut into long 1 ~ 3mm segment;
(2) get broken section of Rhizoma Atractylodis, be placed in extractor, with distilled water immersion, and agitating solution;
(3) the enzyme reagent then adding Rhizoma Atractylodis broken section of weight 0.1% ~ 0.5% carries out enzymolysis processing;
(4) after enzymolysis completes, adopt the technique of vapor distillation, collect distillate;
(5) add distillate volume 2 ~ 5 times of organic solvent extractions, and add the desiccant dehydration of Rhizoma Atractylodis broken section of weight 1/5 ~ 1/4;
(6) adopt heating in water bath to reclaim organic solvent, obtain Rhzoma Atractylodis Lanceae volatile oil.
Adding distilled water and the broken segment body of Rhizoma Atractylodis described in step (2), to amass mass ratio be 5 ~ 10:1, and soak time is 20 ~ 40min.
Enzyme described in step (3) is the one in cellulase, amylase, hemicellulase and pectase, and temperature during enzymolysis is set in 30 ~ 45 DEG C, and enzymolysis time is 1 ~ 3 hour.
Desiccant described in step (5) is the one in anhydrous sodium sulfate, anhydrous magnesium sulfate.
Organic solvent described in step (5) is ether.
Grey volatile oil antineoplastic pharmacologically active prepared by the present invention obtains checking by following pharmacological evaluation.
Select human esophagus cancer cell (EC), human large intestine cancer (HT-29) cell or gastric carcinoma cells (SGC) logarithmic (log) phase cell, after trypsin with 0.4% decomposes cell, individual cells suspension is made into containing in the RPMI-1640 of 10% hyclone, be inoculated in 96 well culture plates, every hole 200 μ L, put 37 DEG C, 5%CO
2condition under cultivate 72 hours, observe under inverted microscope.Experimental group and matched group are set, experimental group renew containing variable concentrations Rhzoma Atractylodis Lanceae volatile oil culture medium (concentration of Rhzoma Atractylodis Lanceae volatile oil is 140.7,100.2,80.6mg/ml), matched group then changes the culture medium containing equal-volume solvent, and often group establishes 3 groups of parallel holes, put 37 DEG C, 5%CO
2condition under cultivate 2 ~ 4 days, centrifugal (1000 turns, 5min), abandoning supernatant.Every hole adds the serum-free medium containing 0.15mg/ml MTT of the fresh configuration of 100 μ L.37 DEG C are continued to cultivate 3h.Careful abandoning supernatant, and add 200 μ LDMSO, after the mixing of miniature ultrasonic agitator, microplate reader measures optical density value to test wavelength for 570nm.And calculate the suppression ratio of different agents amount to tumor cell.The results are shown in following table 1.
Inhibition rate of tumor cell=(1-OD experiment/OD contrasts) * 100%
Table 1: the Rhzoma Atractylodis Lanceae volatile oil of various dose is to the suppression ratio of tumor cell
| The concentration (mg/ml) of Rhzoma Atractylodis Lanceae volatile oil | Human esophagus cancer cell (EC) suppression ratio (%) | Human Large Intestine Carcinoma Cells (HT-29) suppression ratio (%) | People's gastric cancer (SGC) cell inhibitory rate (%) |
| 140.7 | 45.02 | 40.22 | 48.75 |
| 100.2 | 57.56 | 57.03 | 60.41 |
| 80.6 | 23.89 | 28.41 | 20.85 |
As shown in Table 1: Rhizoma Atractylodis extract has obvious growth inhibited effect to human esophagus cancer cell (EC), human large intestine cancer (HT-29) cell and gastric carcinoma cells (SGC), and it can be applied in antitumor product.
Rhzoma Atractylodis Lanceae volatile oil prepared by the present invention and one or more pharmaceutically acceptable solids or liquid excipient or adjuvant are combined, and make the dosage form that applicable human or animal is applicable to.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to following embodiments.
Detailed description of the invention
Embodiment 1:
First the Rhizoma Atractylodis that clean up are removed root, be cut into the segment that length is 2mm, get broken section of 100g Rhizoma Atractylodis, add the distilled water immersion 30 minutes of 500 milliliters, period used Glass rod agitating solution every 10 minutes.Soak after 30 minutes, add 0.1g cellulase, enzymolysis 1 hour under the condition of 30 DEG C.After enzymolysis completes, adopt vapor distillation, collect distillate, add the extracted with diethyl ether of distillate volume 3 times, and add 20g anhydrous sodium sulfate dehydration, ether is reclaimed in 37 DEG C of water-baths, obtains Rhzoma Atractylodis Lanceae volatile oil.
Embodiment 2:
First the Rhizoma Atractylodis that clean up are removed root, be cut into the segment that length is 2mm, get broken section of 100g Rhizoma Atractylodis, add the distilled water immersion 40 minutes of 750 milliliters, period used Glass rod agitating solution every 10 minutes.Soak after 40 minutes, add 0.3g cellulase, enzymolysis 2 hours under the condition of 35 DEG C.After enzymolysis completes, adopt vapor distillation, collect distillate, add the extracted with diethyl ether of distillate volume 5 times, and add 23g anhydrous sodium sulfate dehydration, ether is reclaimed in 40 DEG C of water-baths, obtains Rhzoma Atractylodis Lanceae volatile oil.
Embodiment 3:
First the Rhizoma Atractylodis that clean up are removed root, be cut into the segment that length is 2mm, get broken section of 100g Rhizoma Atractylodis, add the distilled water immersion 40 minutes of 1000ml milliliter, period used Glass rod agitating solution every 10 minutes.Soak after 40 minutes, add 0.5g cellulase, enzymolysis 3 hours under the condition of 45 DEG C.After enzymolysis completes, adopt vapor distillation, collect distillate, add the extracted with diethyl ether of distillate volume 5 times, and add 25g anhydrous sodium sulfate dehydration, ether is reclaimed in 40 DEG C of water-baths, obtains Rhzoma Atractylodis Lanceae volatile oil.
Embodiment 4:
Rhizoma Atractylodis extract makes injection
In obtained Rhzoma Atractylodis Lanceae volatile oil, add water, ethanol or their mixture as solvent, and add lecithin as solubilizing agent, acetate is as pH adjusting agent, and namely glucose can be made into injection as osmotic pressure regulator.
Embodiment 5:
Rhizoma Atractylodis extract makes lyophilized injectable powder
In obtained Rhzoma Atractylodis Lanceae volatile oil, add mannitol, glucose as proppant, make lyophilized injectable powder.
Claims (7)
1. a preparation method for Rhzoma Atractylodis Lanceae volatile oil, is characterized in that it mainly adopts following steps:
(1) fresh Rhizoma Atractylodis are cleaned up, remove root, be cut into long 1 ~ 3mm segment;
(2) get broken section of Rhizoma Atractylodis, be placed in extractor, with distilled water immersion, and agitating solution;
(3) the enzyme reagent then adding Rhizoma Atractylodis broken section of weight 0.1% ~ 0.5% carries out enzymolysis processing;
(4) after enzymolysis completes, adopt the technique of vapor distillation, collect distillate;
(5) add distillate volume 2 ~ 5 times of organic solvent extractions, and add the desiccant dehydration of Rhizoma Atractylodis broken section of weight 1/5 ~ 1/4;
(6) adopt heating in water bath to reclaim organic solvent, obtain Rhzoma Atractylodis Lanceae volatile oil.
2. the preparation method of a kind of Rhzoma Atractylodis Lanceae volatile oil according to claim 1, is characterized in that: adding distilled water and the broken segment body of Rhizoma Atractylodis described in step (2), to amass mass ratio be 5 ~ 10:1, and soak time is 20 ~ 40min.
3. the preparation method of a kind of Rhzoma Atractylodis Lanceae volatile oil according to claim 1, it is characterized in that: the enzyme described in step (3) is the one in cellulase, amylase, hemicellulase and pectase, temperature during enzymolysis is set in 30 ~ 45 DEG C, and enzymolysis time is 1 ~ 3 hour.
4. the preparation method of a kind of Rhzoma Atractylodis Lanceae volatile oil according to claim 1, is characterized in that: the desiccant described in step (5) is the one in anhydrous sodium sulfate, anhydrous magnesium sulfate.
5. the preparation method of a kind of Rhzoma Atractylodis Lanceae volatile oil according to claim 1, is characterized in that: the organic solvent described in step (5) is ether.
6. the Rhzoma Atractylodis Lanceae volatile oil prepared according to method described in claim 1 ~ 5 is preparing the application in antitumor product.
7. antitumor product according to claim 6 is medicine, health product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907476A (en) * | 2016-06-20 | 2016-08-31 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for extracting lentinula edodes essential oil through step-by-step composite enzymatic hydrolysis and steam distillation |
| CN111826235A (en) * | 2020-03-27 | 2020-10-27 | 湖北中医药大学 | Method for extracting rhizoma atractylodis volatile oil |
| CN114058020A (en) * | 2021-11-20 | 2022-02-18 | 江苏康爱特环境工程集团有限公司 | Preparation method and application of quaternary phosphonium salt |
-
2015
- 2015-05-22 CN CN201510263888.2A patent/CN104906162A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907476A (en) * | 2016-06-20 | 2016-08-31 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for extracting lentinula edodes essential oil through step-by-step composite enzymatic hydrolysis and steam distillation |
| CN111826235A (en) * | 2020-03-27 | 2020-10-27 | 湖北中医药大学 | Method for extracting rhizoma atractylodis volatile oil |
| CN114058020A (en) * | 2021-11-20 | 2022-02-18 | 江苏康爱特环境工程集团有限公司 | Preparation method and application of quaternary phosphonium salt |
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Application publication date: 20150916 |