CN106244417A - A kind of device for a large amount of plasmid extraction of high flux and manufacture method thereof - Google Patents
A kind of device for a large amount of plasmid extraction of high flux and manufacture method thereof Download PDFInfo
- Publication number
- CN106244417A CN106244417A CN201610619132.1A CN201610619132A CN106244417A CN 106244417 A CN106244417 A CN 106244417A CN 201610619132 A CN201610619132 A CN 201610619132A CN 106244417 A CN106244417 A CN 106244417A
- Authority
- CN
- China
- Prior art keywords
- liquor stream
- waste liquor
- placing trough
- sample placing
- large amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Abstract
The present invention relates to a kind of device for a large amount of plasmid extraction of high flux and manufacture method thereof, this device includes sample placing trough, waste liquor stream ejecting plate and waste collection bucket, described sample placing trough arranges multiple along short transverse and is ladder-like arranged, sample placing trough is provided with multiple sample putting hole, in addition to the sample placing trough of the top, it is equipped with waste liquor stream ejecting plate at other sample placing troughs, waste liquor stream ejecting plate is provided with multiple waste liquor stream and portals, and waste liquid is portalled by waste liquor stream and flows into waste collection bucket.The present invention uses the hard methacrylate sheet that performance is remarkable, has acid-alkali-corrosive-resisting, wear-resisting, pressure, good mechanical property, the feature such as is unlikely to deform, and device performance is remarkable.The present invention can realize sample prepared by single a large amount of plasmid extraction of batch processed, has the function of eluting and collection concurrently, it is achieved that high flux prepared by a large amount of plasmids, high efficiency, also substantially increases reagent utilization rate simultaneously, reduces human cost.
Description
Technical field
The present invention relates to plasmid in large scale extracting preparation field, a kind of dress for a large amount of plasmid extraction of high flux
Put and manufacture method.
Background technology
During carrying out a large amount of plasmid extraction, need with adsorption column sample being adsorbed, washs, desorption,
Collect；The most a large amount of plasmid extraction, are all the magnanimous process of single sample, significantly consume manpower and materials, improve human cost of knowing clearly,
Also reduce work efficiency.
Summary of the invention
It is an object of the invention to: a kind of device for a large amount of plasmid extraction of high flux and manufacture method thereof are provided, logical
Cross the mode of high flux batch processed, it is achieved to the absorption of plasmid extraction sample, washing, desorption, collection；
In order to solve above-mentioned technical problem, the technical solution used in the present invention is:
A kind of device for a large amount of plasmid extraction of high flux, including sample placing trough, waste liquor stream ejecting plate and waste collection
Bucket, described sample placing trough arranges multiple along short transverse and is ladder-like arranged, and sample placing trough is provided with multiple sample
Putting hole, in addition to the sample placing trough of the top, is equipped with waste liquor stream ejecting plate, on waste liquor stream ejecting plate at other sample placing troughs
Being provided with multiple waste liquor stream to portal, waste liquid is portalled by waste liquor stream and flows into waste collection bucket.
Preferably, described waste liquor stream ejecting plate is square plate body, and waste liquor stream ejecting plate is arranged on last layer sample placing trough
Underface, the upper surface of waste liquor stream ejecting plate flushes with the groove top of sample placing trough.
Preferably, described sample placing trough is at least provided with 2, and described sample placing trough is provided with 26 samples and places
Hole.
Preferably, described sample putting hole and waste liquor stream portal and are circular hole.
Preferably, the material of this device is hard lucite.
Preferably, the manufacture method of a kind of device for a large amount of plasmid extraction of high flux, comprise the following steps:
S1, cutting, process hard lucite finish turning according to size, and cut lengths require that tolerance is ± 0.1mm；
S2, finish turning and open location hole, to cutting after hard lucite finish turning, bottom land and the waste liquor stream to sample placing trough
Ejecting plate opens circular hole, perforate precision ± 0.1mm；
S3, assembling, use lucite glue by finish turning and hold successfully location hole part assemble.
The beneficial effects of the present invention is:
1, the present invention uses the hard methacrylate sheet that performance is remarkable, has acid-alkali-corrosive-resisting, wear-resisting, pressure, mechanical
The features such as performance is good, be unlikely to deform, device performance is remarkable.
2, the present invention can realize sample prepared by single a large amount of plasmid extraction of batch processed, has the function of eluting and collection concurrently,
Achieve high flux, high efficiency prepared by a large amount of plasmid, also substantially increase reagent utilization rate simultaneously, reduce human cost.
Accompanying drawing explanation
The present invention is further detailed explanation with detailed description of the invention below in conjunction with the accompanying drawings.
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the flow chart of manufacture method of the present invention；
Reference: 10-sample placing trough, 11-sample putting hole, 20-waste liquor stream ejecting plate, 21-waste liquor stream portal, 30-
Waste collection bucket.
Detailed description of the invention
It is as follows that embodiments of the present invention is described in detail below:
A kind of device for a large amount of plasmid extraction of high flux, including sample placing trough 10, waste liquor stream ejecting plate 20 and waste liquid
Collecting vessel 30, described sample placing trough 10 arranges multiple along short transverse and is ladder-like arranged, and sample placing trough 10 sets
There is multiple sample putting hole 11, in addition to the sample placing trough 10 of the top, at other sample placing troughs 10, be equipped with waste liquor stream
Ejecting plate 20, waste liquor stream ejecting plate 20 is provided with multiple waste liquor stream and portals 21, and waste liquid is portalled 21 inflow waste collection buckets by waste liquor stream
30。
Preferably, described waste liquor stream ejecting plate 20 is square plate body, and waste liquor stream ejecting plate 20 is arranged on last layer sample and puts
Putting the underface of groove 10, the upper surface of waste liquor stream ejecting plate 20 flushes with the groove top of sample placing trough 10.
Preferably, described sample placing trough 10 is at least provided with 2, and in the schematic diagram of the present invention, sample placing trough 10 is arranged
3, described sample placing trough 10 is provided with 26 sample putting holes 11.
Preferably, described sample putting hole 11 and waste liquor stream portal and 21 are circular hole.
Preferably, the material of this device is hard lucite.
Preferably, the manufacture method of a kind of device for a large amount of plasmid extraction of high flux, comprise the following steps:
S1, cutting, process hard lucite finish turning according to size, and cut lengths require that tolerance is ± 0.1mm；
S2, finish turning and open location hole, to cutting after hard lucite finish turning, bottom land and the waste liquid to sample placing trough 10
Flow out plate 20 and open circular hole, perforate precision ± 0.1mm；
S3, assembling, use lucite glue by finish turning and hold successfully location hole part assemble.
The work process of apparatus of the present invention is as follows:
During real work, Plasmid samples to be purified is placed on the sample putting hole 11 in sample placing trough 10, first
First, using eluent A to clean the impurity on Plasmid samples to be purified, the waste liquid after eluting flows into useless by waste liquor stream ejecting plate 20
It is collected in liquid collecting vessel 30, then collection test tube is placed on waste liquor stream and portals on 21, the sample after remove impurity adds
Eluent B, collects target sample.
Invention described above embodiment, is not intended that limiting the scope of the present invention.Any in the present invention
Spirit and principle within amendment, equivalent and the improvement etc. made, should be included in the claim protection model of the present invention
Within enclosing.
Claims (6)
1. the device for a large amount of plasmid extraction of high flux, it is characterised in that: include sample placing trough, waste liquor stream ejecting plate and
Waste collection bucket, described sample placing trough arranges multiple along short transverse and is ladder-like arranged, and sample placing trough is provided with
Multiple sample putting holes, in addition to the sample placing trough of the top, are equipped with waste liquor stream ejecting plate, waste liquid at other sample placing troughs
Outflow plate is provided with multiple waste liquor stream and portals, and waste liquid is portalled by waste liquor stream and flows into waste collection bucket.
Device for a large amount of plasmid extraction of high flux the most according to claim 1, it is characterised in that: described waste liquor stream
Ejecting plate is square plate body, and waste liquor stream ejecting plate is arranged on the underface of last layer sample placing trough, the upper surface of waste liquor stream ejecting plate
Flush with the groove top of sample placing trough.
Device for a large amount of plasmid extraction of high flux the most according to claim 1, it is characterised in that: described sample is put
Putting groove at least provided with 2, described sample placing trough is provided with 26 sample putting holes.
Device for a large amount of plasmid extraction of high flux the most according to claim 1, it is characterised in that: described sample is put
Put hole and waste liquor stream portals and is circular hole.
Device for a large amount of plasmid extraction of high flux the most according to claim 1, it is characterised in that: the material of this device
For hard lucite.
6. the manufacture method for the device of a large amount of plasmid extraction of high flux, it is characterised in that comprise the following steps:
S1, cutting, process hard lucite finish turning according to size, and cut lengths require that tolerance is ± 0.1mm；
S2, finish turning and open location hole, to cutting after hard lucite finish turning, bottom land and the waste liquor stream ejecting plate to sample placing trough
Open circular hole, perforate precision ± 0.1mm；
S3, assembling, use lucite glue by finish turning and hold successfully location hole part assemble.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610619132.1A CN106244417A (en) | 2016-08-01 | 2016-08-01 | A kind of device for a large amount of plasmid extraction of high flux and manufacture method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610619132.1A CN106244417A (en) | 2016-08-01 | 2016-08-01 | A kind of device for a large amount of plasmid extraction of high flux and manufacture method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106244417A true CN106244417A (en) | 2016-12-21 |
Family
ID=57606775
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610619132.1A Pending CN106244417A (en) | 2016-08-01 | 2016-08-01 | A kind of device for a large amount of plasmid extraction of high flux and manufacture method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106244417A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201609601U (en) * | 2010-02-03 | 2010-10-20 | 云南省烟草农业科学研究院 | Multifunctional solid phase extraction framework |
| CN201974327U (en) * | 2011-02-11 | 2011-09-14 | 天津市宝坻区人民医院 | Staining rack for smear and tissue section |
| CN203075997U (en) * | 2013-02-07 | 2013-07-24 | 陕西理工学院 | Test tube rack with improved structure |
| CN203196378U (en) * | 2013-04-26 | 2013-09-18 | 苏州东奇生物科技有限公司 | Foldable solid-phase extraction device |
| CN103418453A (en) * | 2013-07-31 | 2013-12-04 | 南京佳业检测工程有限公司 | Drip tube frame facilitating waste liquor discharge |
| US20140322806A1 (en) * | 2013-04-30 | 2014-10-30 | Corning Incorporated | Spheroid cell culture well article and methods thereof |
| CN204543640U (en) * | 2015-03-03 | 2015-08-12 | 无锡市疾病预防控制中心 | A kind of device of the SPE for sample pre-treatments |
| CN205216891U (en) * | 2015-12-10 | 2016-05-11 | 内蒙古蒙牛乳业（集团）股份有限公司 | Extraction frame |
-
2016
- 2016-08-01 CN CN201610619132.1A patent/CN106244417A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201609601U (en) * | 2010-02-03 | 2010-10-20 | 云南省烟草农业科学研究院 | Multifunctional solid phase extraction framework |
| CN201974327U (en) * | 2011-02-11 | 2011-09-14 | 天津市宝坻区人民医院 | Staining rack for smear and tissue section |
| CN203075997U (en) * | 2013-02-07 | 2013-07-24 | 陕西理工学院 | Test tube rack with improved structure |
| CN203196378U (en) * | 2013-04-26 | 2013-09-18 | 苏州东奇生物科技有限公司 | Foldable solid-phase extraction device |
| US20140322806A1 (en) * | 2013-04-30 | 2014-10-30 | Corning Incorporated | Spheroid cell culture well article and methods thereof |
| CN103418453A (en) * | 2013-07-31 | 2013-12-04 | 南京佳业检测工程有限公司 | Drip tube frame facilitating waste liquor discharge |
| CN204543640U (en) * | 2015-03-03 | 2015-08-12 | 无锡市疾病预防控制中心 | A kind of device of the SPE for sample pre-treatments |
| CN205216891U (en) * | 2015-12-10 | 2016-05-11 | 内蒙古蒙牛乳业（集团）股份有限公司 | Extraction frame |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161221 |