CN106226465A - A kind of method and device of Accurate Determining double-side photic blade light intensity - Google Patents

A kind of method and device of Accurate Determining double-side photic blade light intensity Download PDF

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Publication number
CN106226465A
CN106226465A CN201610519685.XA CN201610519685A CN106226465A CN 106226465 A CN106226465 A CN 106226465A CN 201610519685 A CN201610519685 A CN 201610519685A CN 106226465 A CN106226465 A CN 106226465A
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intensity
blade
illumination
light source
light
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CN106226465B (en
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郭峰
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SHANGHAI AGRIPHENO AGRICULTURAL TECHNOLOGY Co Ltd
ZEALQUEST SCIENTIFIC TECHNOLOGY Co Ltd
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SHANGHAI AGRIPHENO AGRICULTURAL TECHNOLOGY Co Ltd
ZEALQUEST SCIENTIFIC TECHNOLOGY Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098Plants or trees

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
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  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)

Abstract

The invention discloses the method and device of a kind of Accurate Determining double-side photic blade light intensity, the method includes that S1 measures the first mensuration light source to the intensity of incident illumination between blade one side;S2 measures the first mensuration light source through the intensity of emergent light after blade one side;S3 measures the second mensuration light source to the intensity of the incident illumination between blade another side;S4 measures the second mensuration light source through the intensity of emergent light after vacuum side of blade another side;S5 calculates according to S1 S4 and obtains the actual intensity of illumination that described blade receives.The present invention can not only meet mensuration two-sided to blade, additionally it is possible to accurately reflects the actual light reception intensity of blade, and then improves the precision measured.

Description

A kind of method and device of Accurate Determining double-side photic blade light intensity
Technical field
Plant physiology analysis technical field of the present invention, a kind of method of Accurate Determining double-side photic blade light intensity and dress Put.
Background technology
The i.e. luminous energy anabolic effect of photosynthesis, refers to containing chloroplast green plants, animal and some antibacterial, at visible ray Irradiation under, react (being once called as dark reaction) through photoreaction and carbon, utilize photosynthetic pigments, by carbon dioxide (or hydrogen sulfide) and water It is converted into Organic substance, and discharges the biochemical process of oxygen (or hydrogen), be also most importantization in plant physiology metabolic process Learn reaction.And photosynthetic measurement and research always plant physiology, phytoecology, agronomy, forestry, gardening, algae The focus in the fields such as class physiological ecology.
The photosynthesis of plant can be affected by multiple environmental factors, and wherein light reception intensity is that impact is photosynthetic heavy Want factor.Illumination is that plant leaf blade carries out photosynthetic energy source, and the strong of illumination directly affects the photosynthetic of blade with weak meeting Speed.Same blade is under different light reception intensity, and its photosynthetic rate is the most different.The most photosynthetic measuring instrument, as Photosynthetic instrument, luminoscope etc., be required for measuring the light reception intensity of blade while measuring photosynthetic rate.If can not Accurate Determining The light reception intensity of blade, will seriously reduce the using value of photosynthetic measuring instrument.
At present, the method measuring blade light reception intensity is to place a light quantum sensor on blade side to be measured, passes through The intensity measuring incident illumination represents the light reception intensity of blade.But this method is only capable of recording the light reception intensity of blade surface, with Raising and the progress of determination techniques that certainty of measurement requires, some photosynthetic measurement equipment needs from front-back two-sided simultaneously to blade Irradiation.Under these conditions, the actual light situation of blade just becomes more complicated, only by measuring the intensity of incident illumination, nothing Opinion is that one side measures or two-sided mensuration, all can not accurately reflect the actual light reception intensity of blade, and this has become the complicated light of research Under the conditions of according to, photosynthesis measures the technical bottleneck of equipment.For this research equipment higher to required precision, will have a strong impact on Its application effect and using value.
Summary of the invention
It is an object of the invention to provide the method and device of a kind of Accurate Determining double-side photic blade light intensity, can not only expire The two-sided mensuration of foot, additionally it is possible to accurately reflect the actual light reception intensity of blade, and then improve the precision measured.
The technical scheme that the present invention provides is as follows:
A kind of method of Accurate Determining double-side photic blade light intensity, it is characterised in that including:
S1 measures the first mensuration light source to the intensity of incident illumination between blade one side;
S2 measures the first mensuration light source through the intensity of emergent light after blade one side;
S3 measures the second mensuration light source to the intensity of the incident illumination between blade another side;
S4 measures the second mensuration light source through the intensity of emergent light after blade another side;
S5 calculates according to S1-S4 and obtains the actual intensity of illumination that described blade receives.
The technical program is by the intensity of the incident illumination between the first mensuration light source of measurement to blade one side, and passes through The intensity of emergent light after blade one side, the most also measures strong to the incident illumination between blade another side of the second mensuration light source Degree, and through the intensity of emergent light after blade another side, it is possible to calculate the actual intensity of illumination that blade receives, reach double Face measures, additionally it is possible to reflects the actual light reception intensity of blade exactly, and then improves the precision measured.
Preferably, obtaining described blade one side top layer intensity of illumination according to S1 and S4, this intensity of illumination is described blade one The intensity of face incident illumination and the intensity summation of described blade another side emergent light.
Preferably, obtaining described blade another side top layer intensity of illumination according to S2 and S3, this intensity of illumination is described blade The intensity of another side incident illumination and the intensity summation of described one emergent light of blade.
Owing to blade has certain light transmission, therefore blade one side top layer light intensity is intensity and emergent light strong of incident illumination The summation of degree.Same blade another side top layer intensity of illumination is the intensity summation with the intensity of emergent light of incident illumination.
Preferably, the actual intensity of illumination of described blade is the average intensity of illumination of described blade.
The actual light intensity received due to blade is the meansigma methods of whole blade light intensity, so by calculating whole blade light Strong meansigma methods, i.e. can get the actual light intensity that whole blade receives.
Preferably, when the one side of described blade is injected by described first mensuration light source, the another side of described blade is subject to To described second measure light source inject time, the calculation of the actual intensity of illumination of described blade is:
a + b ′ + b + a ′ 2
Wherein, a is the first mensuration light source to the intensity of the incident illumination between blade one side;A ' is that the first mensuration light source passes through The intensity of emergent light after blade one side;B is the second mensuration light source to the intensity of the incident illumination between blade another side;B ' second surveys Determine light source through the intensity of emergent light after blade another side.
Preferably, when the one side of described blade is injected by described first mensuration light source, the another side of described blade enters The intensity penetrating light is zero, and the calculation of the actual intensity of illumination of described blade is:
a + a ′ 2
Wherein, a is the first mensuration light source to the intensity of the incident illumination between blade one side;A ' is that the first mensuration light source passes through The intensity of emergent light after blade one side.
The present invention additionally provides the device of a kind of Accurate Determining double-side photic blade light intensity, including:
First intensity of illumination measurement apparatus, the intensity of the incident illumination between measurement the first mensuration light source to blade one side And shown with data mode by this illumination intensity value measured, and measure the first mensuration light source through emergent light after blade one side Intensity and by measure this illumination intensity value show with data mode;
Second intensity of illumination measurement apparatus, for measuring strong to the incident illumination between blade another side of the second mensuration light source This illumination intensity value of degree show with data mode, and measures the second mensuration light source and pass through the strong of emergent light after blade another side This illumination intensity value of degree shows with data mode.
In the technical program, the first intensity of illumination measurement apparatus and the second intensity of illumination measurement apparatus by arranging are carried out Measure, and obtain concrete intensity of illumination data.
Preferably, described first intensity of illumination measurement apparatus and described second intensity of illumination measurement apparatus are arranged at described The both sides of blade, and it is respectively facing described first mensuration light source and described second mensuration light source.
First intensity of illumination measurement apparatus and the second intensity of illumination measurement apparatus are arranged on the both sides of blade, and will be towards The most each towards measuring light source, the intensity of illumination that blade is an actually-received can be measured as far as possible, and then it is strong to improve illumination The measurement accuracy of degree.
Preferably, described first intensity of illumination measurement apparatus and described second intensity of illumination measurement apparatus specifically use photosynthetic One in instrument, luminoscope or light quantum meter.
By the method and device of a kind of Accurate Determining double-side photic blade light intensity that the present invention provides, it is possible to bring following At least one beneficial effect:
1, the intensity of illumination measurement apparatus that the present invention is arranged by blade both sides, it is achieved same to the intensity of illumination of blade both sides Time measure, effectively calculate the actual light reception intensity of blade by the intensity of illumination of two-sided measurement gained.
2, using the actual intensity of illumination of blade in the present invention is blade this feature of average intensity of illumination, thus calculates leafing The actual intensity of illumination of sheet.And measurement result can directly be shown by intensity of illumination measurement apparatus in the form of data, test Personnel intuitively can be accurately obtained relevant intensity of illumination data.
3, two intensity of illumination measurement apparatus are arranged on blade both sides by the present invention, the accuracy of measurement can be improved, And then improve the final blade actual light of gained that calculates according to strength values.
4, measuring method of the present invention is simple, and the blade actual light that survey calculation obtains is high according to strength values accuracy, more excellent Be to break through existing intensity of illumination measurement apparatus to cannot be carried out the drawback of two planar survey.
Accompanying drawing explanation
Below by the way of the most understandable, accompanying drawings preferred implementation, Accurate Determining is two-sided is subject to a kind of Above-mentioned characteristic, technical characteristic, advantage and the implementation thereof of the method and device of light blade light intensity are further described.
Fig. 1 is the key step schematic diagram of the method for the present invention a kind of Accurate Determining double-side photic blade light intensity;
Fig. 2 is the meaning of a specific embodiment of the method measurement of the present invention a kind of Accurate Determining double-side photic blade light intensity Figure;
Fig. 3 is another specific embodiment of the method measurement of the present invention a kind of Accurate Determining double-side photic blade light intensity It is intended to.
Drawing reference numeral illustrates:
First measures light source A;Second measures light source B;Blade C.
Detailed description of the invention
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will comparison accompanying drawing explanation The detailed description of the invention of the present invention.It should be evident that the accompanying drawing in describing below is only some embodiments of the present invention, for From the point of view of those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to obtain other according to these accompanying drawings Accompanying drawing, and obtain other embodiment.
For more clear explanation, the arrow in accompanying drawing 2,3 of the present invention be light inject direction.
The method of the present invention a kind of Accurate Determining double-side photic blade light intensity is measured mainly by existing intensity of illumination Device is monitored, and intensity of illumination measurement apparatus is arranged on the both sides of blade, for key structure and the merit of the prominent present invention Can, the mounting means concrete for intensity of illumination measurement apparatus omits.
As one specific embodiment of the present invention, as it is shown in figure 1, the side of a kind of Accurate Determining double-side photic blade light intensity Method, including the intensity of the incident illumination between S1 measurement the first mensuration light source to blade one side (one side that i.e. light application time is longer); S2 measures the first mensuration light source through the intensity of emergent light after blade one side;S3 measures the second mensuration light source to blade another side The intensity of the incident illumination between (one side that i.e. light application time is shorter);S4 measures the second mensuration light source and goes out through after blade another side Penetrate the intensity of light;S5 calculates, according to S1-S4, the actual intensity of illumination that blade receives.
The actual intensity of illumination of blade of the present invention is according to blade one side and the intensity of another side incident illumination, and through blade Emergent light Strength co-mputation obtain.The reality of blade can be accurately reflected by intensity of illumination, and then improve the precision measured. Effectively judge the intensity of illumination suffered by blade, provide light intensity data accurately for photosynthetic measurement equipment, make photosynthetic measuring instrument The data acquisition of device closer to the reality of blade by intensity of illumination.
During concrete measurement, strong to the incident illumination between blade one side of the first mensuration light source can be measured according to step S1 Degree;And S4 measures the second mensuration light source through the intensity of emergent light after blade another side, calculating acquisition blade one side top layer light According to intensity, blade one side top layer intensity of illumination is (the i.e. intensity of one incident illumination of blade and the blade another side outgoing of both summations The summation of the intensity of light);Also can measure the first mensuration light source through the intensity of emergent light after blade one side according to S2;And S3 surveys Flow control two measures light source to the intensity of the incident illumination between blade another side, and calculating blade another side top layer intensity of illumination is two Person's summation (i.e. the intensity of blade another side incident illumination and the intensity summation of one emergent light of blade), calculates finally by step S5 Obtain the actual intensity of illumination that described blade receives.
It should be noted that owing to the actual intensity of illumination of blade C is the average intensity of illumination that blade C Surface is subject to, therefore, Can directly calculate according to two summations obtained by above-mentioned calculating and can obtain the actual intensity of illumination of blade C.
And blade C to be measured is placed in the first mensuration light source A and second and measures in the middle of two light sources of light source B, respectively from blade C mono- Face and another side are to light.And the first brightness measuring light source A and second mensuration two light sources of light source B may identical be likely to not With, and blade C is likely located at two light source middles, it is also possible to measure light source apart from one of them relatively near, all can use the party Method determines the actual light intensity that blade C receives.
In the embodiment one of concrete survey calculation:
Referring to shown in Fig. 2, when blade C one side by first measure light source A inject time, the another side of blade C is by the Two measure light source B injects, and the calculation of the actual intensity of illumination of blade C is:
a + b ′ + b + a ′ 2
Wherein it should be noted that the intensity that a is the incident illumination between the first mensuration light source A to blade C one side;A ' is first Measure light source A through the intensity of emergent light after blade C one side;B is the second mensuration light source B to the incidence between blade C another side The intensity of light;B ' is that the second mensuration light source B is through the intensity of emergent light after blade C another side.
In the embodiment two of concrete survey calculation:
When the one side of blade C is injected by the first mensuration light source A, the another side of blade C measures light source B without second and injects Time, the calculation of the actual intensity of illumination of blade C is:
a + a ′ 2
Wherein it should be noted that the intensity that a is the incident illumination between the first mensuration light source A to blade C one side;A ' is first Measure light source A through the intensity of emergent light after blade C one side.
Incident intensity is measured merely, it may be assumed that leaf as used during according to the light intensity of prior art mensuration unilateral irradiation blade The actual light intensity that sheet C accepts is a.
Therefore, use the method for above-mentioned measurement can be additionally used in and revise the error that existing unilateral light intensity assay method brings, make It is more accurate, and then improves the accuracy of measurement data, is shown in Table 1.
The concrete measured data of table 1:
From measured data it can be seen that the determination techniques of existing simple incident intensity cannot accurately reflect blade actual light By force, measuring method of the present invention can reflect blade actual light situation more truely and accurately.By the present invention, can be light Cooperation measurement equipment provides light intensity data accurately.
It should be noted that the data obtained by above-mentioned table 1, be that the scientific research apparatus that blade C is put into the double light source of band enters Row is measured, and then may determine that the actual light intensity of blade.
And the blade grown in nature, actual blade can receive the incident illumination of both direction: blade is simultaneously Accepting the direct irradiation of sunlight, blade another side can be by the reflective irradiation in ground.Although the reflective intensity in ground is less than sunlight Direct projection, but still the photosynthesis of blade can be produced impact.Ground intensity of light reflection can be by ground material, soil constituent, aqueous The impact of the many factors such as amount, covering so that the light situation of blade becomes increasingly complex.And how to determine in nature raw The actual intensity of illumination of long blade, still can adopt and measure with the aforedescribed process.
Referring to shown in Fig. 3, use the method for above-mentioned measurement still can obtain the actual intensity of illumination of blade C more accurately, It is shown in Table 2.
The concrete measured data of table 2:
From measured data it can be seen that the determination techniques of existing simple incident intensity cannot accurately reflect blade actual light According to intensity, the blade actual light intensity in natural environment can be affected by ground intensity of light reflection and blade printing opacity situation own.This Measuring method described in invention can reflect blade actual light situation more truely and accurately.By the present invention, it can be light cooperation Light intensity data accurately is provided with measurement equipment.
Additionally providing the device of a kind of Accurate Determining double-side photic blade light intensity in the present invention, concrete includes the first light According to strength meter (not shown), for measuring the intensity of the incident illumination between the first mensuration light source A to blade C one side And shown with data mode by this illumination intensity value measured, and measure the first mensuration light source A through outgoing after blade C one side This illumination intensity value measured also is shown by the intensity of light with data mode;It is also provided with the second intensity of illumination measurement apparatus simultaneously (not shown), this intensity of illumination of the intensity of the incident illumination between measurement the second mensuration light source B to blade C another side Value shows with data mode, and it is strong through this illumination of the intensity of emergent light after blade C another side to measure the second mensuration light source B Angle value shows with data mode.
In the device embodiment of the Accurate Determining double-side photic blade light intensity of the present invention, the first intensity of illumination measurement apparatus and Second intensity of illumination measurement apparatus is arranged at the both sides of blade C, and is respectively facing the first mensuration light source A and second mensuration light source B.It should be noted that the concrete mounting means of the first intensity of illumination measurement apparatus and the second intensity of illumination measurement apparatus does not do into one The restriction of step, it is only necessary to ensure to be close to blade C, so can further ensure that the intensity of illumination of measurement is close to blade C Suffered actual intensity of illumination.
Of coursing in other embodiments, the first intensity of illumination measurement apparatus and the second intensity of illumination measurement apparatus can be adopted By the one in the photosynthetic instrument that can directly buy in the market, luminoscope or light quantum meter.
It should be noted that, above-described embodiment all can independent assortment as required.The above is only the preferred of the present invention Embodiment, it is noted that for those skilled in the art, in the premise without departing from the principle of the invention Under, it is also possible to making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.

Claims (9)

1. the method for an Accurate Determining double-side photic blade light intensity, it is characterised in that including:
S1 measures the first mensuration light source to the intensity of incident illumination between blade one side;
S2 measures the first mensuration light source through the intensity of emergent light after blade one side;
S3 measures the second mensuration light source to the intensity of the incident illumination between blade another side;
S4 measures the second mensuration light source through the intensity of emergent light after blade another side;
S5 calculates according to S1-S4 and obtains the actual intensity of illumination that described blade receives.
The method of Accurate Determining double-side photic blade light intensity the most according to claim 1, it is characterised in that:
Obtaining described blade one side top layer intensity of illumination according to S1 and S4, this intensity of illumination is the strong of described one incident illumination of blade Degree and the intensity summation of described blade another side emergent light.
The method of Accurate Determining double-side photic blade light intensity the most according to claim 2, it is characterised in that:
Obtaining described blade another side top layer intensity of illumination according to S2 and S3, this intensity of illumination is described blade another side incident illumination The intensity summation of intensity and described one emergent light of blade.
The method of Accurate Determining double-side photic blade light intensity the most according to claim 3, it is characterised in that:
The actual intensity of illumination of described blade is the average intensity of illumination of described blade.
The method of Accurate Determining double-side photic blade light intensity the most according to claim 3, it is characterised in that:
When the one side of described blade is injected by described first mensuration light source, the another side of described blade is surveyed by described second Determining light source when injecting, the calculation of the actual intensity of illumination of described blade is:
a + b ′ + b + a ′ 2
Wherein, a is the first mensuration light source to the intensity of the incident illumination between blade one side;A ' is that the first mensuration light source is through blade The intensity of emergent light after Yi Mian;B is the second mensuration light source to the intensity of the incident illumination between blade another side;B ' second measures light Source is through the intensity of emergent light after blade another side.
The method of Accurate Determining double-side photic blade light intensity the most according to claim 3, it is characterised in that:
When the one side of described blade is injected by described first mensuration light source, the intensity of the another side incident illumination of described blade is Zero, the calculation of the actual intensity of illumination of described blade is:
a + a ′ 2
Wherein, a is the first mensuration light source to the intensity of the incident illumination between blade one side;A ' is that the first mensuration light source is through blade The intensity of emergent light after Yi Mian.
7. the device of an Accurate Determining double-side photic blade light intensity, it is characterised in that including:
First intensity of illumination measurement apparatus, for measure first mensuration light source to blade one side between incident illumination intensity and by This illumination intensity value measured show with data mode, and measure the first mensuration light source through blade simultaneously after emergent light strong This illumination intensity value measured also is shown by degree with data mode;
Second intensity of illumination measurement apparatus, the intensity of the incident illumination between measurement the second mensuration light source to blade another side This illumination intensity value shows with data mode, and measures the second mensuration light source through the intensity of emergent light after blade another side This illumination intensity value shows with data mode.
The device of Accurate Determining double-side photic blade light intensity the most according to claim 7, it is characterised in that including:
Described first intensity of illumination measurement apparatus and described second intensity of illumination measurement apparatus are arranged at the both sides of described blade, And it is respectively facing described first mensuration light source and described second mensuration light source.
9. according to the device of the Accurate Determining double-side photic blade light intensity described in claim 7 or 8, it is characterised in that including:
Described first intensity of illumination measurement apparatus and described second intensity of illumination measurement apparatus specifically use photosynthetic instrument, luminoscope or One in light quantum meter.
CN201610519685.XA 2016-07-05 2016-07-05 A kind of method and device of Accurate Determining double-side photic blade light intensity Active CN106226465B (en)

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Publication number Priority date Publication date Assignee Title
CN1180839A (en) * 1996-08-01 1998-05-06 株式会社佐竹制作所 Content measuring apparatus for plant leaf
CN1815226A (en) * 2006-02-23 2006-08-09 上海交通大学 Canopy leaf chamber for determining plant canopy population photosynthesis
CN1826050A (en) * 2003-05-23 2006-08-30 斯坦尼斯劳·卡平斯基 Methods for altering the level of phytochemicals in plant cells by applying wavelengths of light from 400 NM to 700 NM and apparatus therefore
CN101556240A (en) * 2009-05-22 2009-10-14 天津大学 Chlorophyll measuring apparatus based on RGB (red, green and blue) digital signal
CN101949825A (en) * 2010-08-17 2011-01-19 中国农业大学 Leaf water near infrared non-destructive testing device and method in light open environment
CN102435590A (en) * 2011-08-30 2012-05-02 上海泽泉科技有限公司 Method for confirming actinic light intensity in chlorophyll fluorescence induction curve measurement
CN104931425A (en) * 2015-05-29 2015-09-23 苏州德锐朗智能科技有限公司 Chlorophyll content detection and control method with temperature and humidity compensation function
CN104931438A (en) * 2015-05-29 2015-09-23 苏州德锐朗智能科技有限公司 Photoelectric sensor-based chlorophyll content detection method

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1180839A (en) * 1996-08-01 1998-05-06 株式会社佐竹制作所 Content measuring apparatus for plant leaf
CN1826050A (en) * 2003-05-23 2006-08-30 斯坦尼斯劳·卡平斯基 Methods for altering the level of phytochemicals in plant cells by applying wavelengths of light from 400 NM to 700 NM and apparatus therefore
CN1815226A (en) * 2006-02-23 2006-08-09 上海交通大学 Canopy leaf chamber for determining plant canopy population photosynthesis
CN101556240A (en) * 2009-05-22 2009-10-14 天津大学 Chlorophyll measuring apparatus based on RGB (red, green and blue) digital signal
CN101949825A (en) * 2010-08-17 2011-01-19 中国农业大学 Leaf water near infrared non-destructive testing device and method in light open environment
CN102435590A (en) * 2011-08-30 2012-05-02 上海泽泉科技有限公司 Method for confirming actinic light intensity in chlorophyll fluorescence induction curve measurement
CN104931425A (en) * 2015-05-29 2015-09-23 苏州德锐朗智能科技有限公司 Chlorophyll content detection and control method with temperature and humidity compensation function
CN104931438A (en) * 2015-05-29 2015-09-23 苏州德锐朗智能科技有限公司 Photoelectric sensor-based chlorophyll content detection method

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