CN106222225B - 一种牛骨活性肽的制备方法 - Google Patents
一种牛骨活性肽的制备方法 Download PDFInfo
- Publication number
- CN106222225B CN106222225B CN201610782329.7A CN201610782329A CN106222225B CN 106222225 B CN106222225 B CN 106222225B CN 201610782329 A CN201610782329 A CN 201610782329A CN 106222225 B CN106222225 B CN 106222225B
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- bone
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- vitamin
- concentrated
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- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 73
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 46
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 42
- 102000004190 Enzymes Human genes 0.000 claims abstract description 30
- 108090000790 Enzymes Proteins 0.000 claims abstract description 30
- 239000000243 solution Substances 0.000 claims description 48
- 230000009849 deactivation Effects 0.000 claims description 22
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 20
- 229930003268 Vitamin C Natural products 0.000 claims description 20
- 239000007864 aqueous solution Substances 0.000 claims description 20
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 20
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 20
- 235000019154 vitamin C Nutrition 0.000 claims description 20
- 239000011718 vitamin C Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000000758 substrate Substances 0.000 claims description 14
- 108091005508 Acid proteases Proteins 0.000 claims description 12
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 11
- 238000005238 degreasing Methods 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 11
- 238000002347 injection Methods 0.000 claims description 11
- 239000007924 injection Substances 0.000 claims description 11
- 239000010451 perlite Substances 0.000 claims description 11
- 235000019362 perlite Nutrition 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 10
- 230000003064 anti-oxidating effect Effects 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 2
- 230000036541 health Effects 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 abstract description 10
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 10
- 238000007654 immersion Methods 0.000 abstract description 3
- 229960005070 ascorbic acid Drugs 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 238000002203 pretreatment Methods 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 8
- 230000002000 scavenging effect Effects 0.000 description 8
- -1 hydroxyl free radical Chemical class 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 239000000047 product Substances 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 239000004327 boric acid Substances 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012263 liquid product Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000001828 Gelatine Substances 0.000 description 1
- 244000225942 Viola tricolor Species 0.000 description 1
- 235000004031 Viola x wittrockiana Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610782329.7A CN106222225B (zh) | 2016-08-31 | 2016-08-31 | 一种牛骨活性肽的制备方法 |
Applications Claiming Priority (1)
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CN201610782329.7A CN106222225B (zh) | 2016-08-31 | 2016-08-31 | 一种牛骨活性肽的制备方法 |
Publications (2)
Publication Number | Publication Date |
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CN106222225A CN106222225A (zh) | 2016-12-14 |
CN106222225B true CN106222225B (zh) | 2019-06-21 |
Family
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Family Applications (1)
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CN201610782329.7A Active CN106222225B (zh) | 2016-08-31 | 2016-08-31 | 一种牛骨活性肽的制备方法 |
Country Status (1)
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CN (1) | CN106222225B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109247584A (zh) * | 2017-07-16 | 2019-01-22 | 北京神飞航天应用技术研究院 | 一种适合航天员营养的牛骨胶原蛋白肽粉及其制备方法 |
CN110679858A (zh) * | 2019-09-30 | 2020-01-14 | 南宁学院 | 一种牛骨中钙营养剂的提取方法 |
CN113854574B (zh) * | 2021-10-11 | 2023-11-10 | 杭州佰倍优生物科技有限公司 | 一种新型复方骨肽及其应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1095904A (zh) * | 1994-04-28 | 1994-12-07 | 安徽三体保健品公司 | 牛骨提取液 |
CN105385737A (zh) * | 2015-12-09 | 2016-03-09 | 青海北极牦牛生物科技有限公司 | 一种牦牛骨胶原寡肽的制备工艺 |
-
2016
- 2016-08-31 CN CN201610782329.7A patent/CN106222225B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1095904A (zh) * | 1994-04-28 | 1994-12-07 | 安徽三体保健品公司 | 牛骨提取液 |
CN105385737A (zh) * | 2015-12-09 | 2016-03-09 | 青海北极牦牛生物科技有限公司 | 一种牦牛骨胶原寡肽的制备工艺 |
Non-Patent Citations (3)
Title |
---|
Preparation and structure characterization of soluble bone collagen peptide chelating calcium.;Yong-Guo Jin et al.;《African Journal of Biotechnology》;20110905;第10卷(第50期);第10204-10211页 |
响应面法优化牛骨制备骨多肽工艺的研究;吕雪鹏等;《食品工业科技》;20160215;第37卷(第3期);第265-270页 |
牛骨胶原多肽螯合钙的制备及其结构表征;付文雯;《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》;20110415(第4期);摘要 |
Also Published As
Publication number | Publication date |
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CN106222225A (zh) | 2016-12-14 |
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C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A preparation method of bovine bone active peptide Effective date of registration: 20230323 Granted publication date: 20190621 Pledgee: China Construction Bank Corporation Jingzhou Yuqiao Branch Pledgor: HUBEI REBORN BIOTECH CO.,LTD. Registration number: Y2023980035961 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20190621 Pledgee: China Construction Bank Corporation Jingzhou Yuqiao Branch Pledgor: HUBEI REBORN BIOTECH CO.,LTD. Registration number: Y2023980035961 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A preparation method of bovine bone active peptide Granted publication date: 20190621 Pledgee: China Construction Bank Corporation Jingzhou Yuqiao Branch Pledgor: HUBEI REBORN BIOTECH CO.,LTD. Registration number: Y2024980012718 |