CN106222182A - The IbERF5 gene of coding sweet potato ERF transcription and application - Google Patents
The IbERF5 gene of coding sweet potato ERF transcription and application Download PDFInfo
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Abstract
The invention discloses IbERF5 in Rhizoma Dioscoreae esculentae and encoding gene thereof and the application in regulation and control plant stress tolerance.The wherein IbERF5 gene of coding sweet potato ERF transcription, is the nucleotide sequence shown in SEQ ID NO.1.The protein of IbERF5 gene code is the aminoacid sequence shown in SEQ ID No.2.The present invention isolates the global cDNA of encoding transcription factors IbERF5 from Rhizoma Dioscoreae esculentae, is connected on plant expression vector, utilizes Agrobacterium infestation method to convert plant, it is thus achieved that transfer-gen plant, and improves drought tolerance and the salt tolerance of transgenic plant.This gene can apply to genetic modification of plants.
Description
Technical field
The invention belongs to genetic engineering field, be specifically related to the IbERF5 gene of a kind of coding sweet potato ERF transcription, be somebody's turn to do
The protein of gene code and the recombinant vector containing this gene and the degeneration-resistant application of gene.
Background technology
In plant, many genes are all by environment stress abduction delivering, according to the difference of model of action, are broadly divided into two big
Class: regulator gene and functional gene.What regulator gene mainly played regulatory role in signal transduction and gene expression transcribe because of
Son；Some regulation osmotic potentials of functional gene main code, removing free radical and the enzyme etc. of active oxygen.It is generally believed that plant is to dry
The resistance of the adverse circumstances such as drought and high salt is by controlled by multiple genes, therefore, although utilizing technique for gene engineering to import individual feature gene energy
Increase certain single resistance of plant, but can not the most comprehensively improve the resistance of plant.Transcription factor is as function
The regulation switch of gene expression, can regulate accurately different genes, sends out in plant adverse circumstance signal transduction process
Wave the effect of key, so by the effect strengthening certain transcription factor, multiple and degeneration-resistant relevant functional gene can be promoted
Expressing, this is the very effective approach making plant stress-resistance character obtain comprehensive improvement.
Plant AP2/ERF is a huge transcription factor gene family, containing be made up of 60～70 aminoacid
AP2/ERF domain and gain the name, be present in all of plant.AP2/ERF transcription factor participates in various biological process, including
The environment-stress responses etc. such as plant growing, flower development, fruit development, seed development, damage, pathogenic bacteria defence, high salt, arid.
AP2/ERF class transcription factor participates in the multi-signal transduction pathway such as salicylic acid, jasmonic, ethylene, abscisic acid, and is adverse circumstance letter
Connection exception in number cross-pathway.According to the number containing AP2/ERF domain, AP2/ERF transcription factor contains 5 subgroups, bag
Include AP2 (APETALA 2), RAV (related to ABI3/VP1), DREB (dehydration-responsive element
Binding protein), ERF and other.AP2 family contains 2 AP2/EREBP (ethylene-responsive element
Binding protein) domain, ERF and DREB family is only containing 1 AP2/EREBP domain, and RAV family is except one
Also containing 1 B3 domain outside AP2/EREBP domain.ERF family is a main sub-family of AP2/ERF transcription factor extended familys
Race, plays a significant role in regulation plant biological and abiotic stress reaction.Arabidopsis and rice genome contain respectively
There are 122 and 139 ERF transcription family members, according to features such as gene order phylogenetic tree and albumen conserved domains, intend south
Mustard separately constitutes 12 groups different with 15 with Oryza sativa L. ERF gene.
Rhizoma Dioscoreae esculentae is not only important cereal crops, but also is important industrial crops and energy crop.Rhizoma Dioscoreae esculentae is extensively planted
Being implanted in more than 100 in the world country, China is largest production state in the world, and Rhizoma Dioscoreae esculentae produces and accounts for the 80% of world Rhizoma Dioscoreae esculentae.
Compared with other cereal crops, Rhizoma Dioscoreae esculentae is the most drought-enduring, but Differences is relatively big, in the world the Rhizoma Dioscoreae esculentae plantation of significant proportion
In arid environments, and the world is arid, semiarid zone has accounted for more than 1/3rd of land surface, the arid shadow to plant
Ring and rank first in many natural stress factors.About 2,000,000,000 mu of China's existing marginal land resource (bare place), the most dry
Beach non-irrigated, saline and alkaline ground.In China, agricultural water is in short supply, under cultivated area intense situation, drought-enduring in the plantation of these regional developments
Rhizoma Dioscoreae esculentae, can reasonably utilize land resource, can partly alleviate again grain and energy scarcity problem.By orderly improvement Rhizoma Dioscoreae esculentae
Degeneration-resistant border ability, improves the Rhizoma Dioscoreae esculentae growth adaptability in marginal land district, has important to the grain security guaranteeing China
Strategic importance.Therefore in Rhizoma Dioscoreae esculentae, clone new ERF class transcription factor gene, study its basic biological characteristics and function,
Can be whole plant stress-resistance gene regulatory network and stress response reaction mechanism is provided fundamental basis, and be Crop Improvement resistance
Certain material base is provided.
Applicant discloses a kind of coding sweet potato ERF transcription in patent of invention (Application No. 2015102103933)
IbERF4 gene and application, at this research on the basis of, applicant has proceeded further research, continually looks for sweet
The gene that potato resistance is relevant, provides basis for Rhizoma Dioscoreae esculentae improvement.
Summary of the invention
It is an object of the invention to provide the IbERF5 gene of a kind of coding sweet potato ERF transcription.
Second object of the present invention is to provide the protein of this gene code a kind of.
Third object of the present invention is to provide a kind of expression vector containing this gene.
Fourth object of the present invention is to provide a kind of host cell containing this expression vector.
Final object of the present invention is to provide the purposes of this gene.
Technical scheme is summarized as follows:
The IbERF5 gene of a kind of coding sweet potato ERF transcription, it is the nucleotide sequence shown in SEQ ID NO.1.
Described nucleotide sequence is by 705 base compositions.
The protein of said gene coding, it is the aminoacid sequence shown in SEQ ID No.2.Described sequence is by 234
Individual amino acid residue forms.
A kind of expression vector pCAMBIA1305-IbERF5 containing said gene, it contains shown in SEQ ID NO.1
Nucleotide sequence.
A kind of structure containing expression vector Agrobacterium host cell EHA105:pCAMBIA1305-2 × 35s-IbERF5.
Said gene application in converting plant acquisition transfer-gen plant, especially in preparing transgenic arabidopsis
Application.
Described IbERF5 gene has enhancing plant salt endurance and the function of drought tolerance.
Advantages of the present invention:
The present invention isolates the global cDNA of coding ERF transcription gene from Rhizoma Dioscoreae esculentae, is connected to plant expression vector
On, utilize Agrobacterium infestation method convert plant, it is thus achieved that transfer-gen plant, transfer-gen plant has been carried out resistance analysis, knot
Fruit shows that IbERF5 gene can strengthen drought-resistance ability and the salt resistance ability of positive regulation plant.This gene can apply to plant
Genetic improvement.
Accompanying drawing explanation
The Phylogenetic analysis result of Fig. 1 .IbERF5 aminoacid sequence
Fig. 2 .IbERF5 gene arid, salt stress abduction delivering
Fig. 3 .pCAMBIA1305-2 × 35s-IbERF5 carrier schematic diagram
Fig. 4. unconverted arabidopsis strain and turn IbERF5 gene plant drought resisting and compare
Fig. 5. unconverted arabidopsis strain and turn the anti-salt of IbERF5 gene plant and compare
Detailed description of the invention
Below in conjunction with specific embodiment further describe the present invention, advantages of the present invention and feature will be with describe and
Apparent.But specific experiment method involved in following embodiment is if no special instructions, is conventional method or according to system
The condition making manufacturers instruction suggestion is implemented.
Embodiment 1
In Rhizoma Dioscoreae esculentae, ERF transcription IbERF5 gene order obtains, specific as follows:
Utilize OMEGA RNA test kit, from the fresh Sweet Potato Leaf of 100mg, extract total serum IgE, utilize Reverse Transcription
Box (Bioteke) synthesis cDNA.
Concrete reaction system is as follows:
PCR instrument carries out reverse transcription reaction by following condition: 1,50 DEG C, 45min；2,70 DEG C, 10min；The most on ice
Cooling.
The total serum IgE of extraction is sent Hua Da gene (Beijing Genomic Institute, BGI), passes through high-flux sequence
Obtain Rhizoma Dioscoreae esculentae transcript profile data base, and obtain Rhizoma Dioscoreae esculentae Unigene data base by De novo splicing, wherein
Find after CL5071.Contig1_All Yu NR data base's comparison that this gene may participate in stress response.Will
CL5071.Contig1_All passes through OFR FINDER (http://www.ncbi.nlm.nih.gov/projects/gorf/)
The ORF that online software prediction gene completes, and utilize the Primer Premier 5 design complete ORF primer of amplification:
IbERF5(F):5'ATGGCTTTATCTGAGGAAAG 3'
IbERF5(R):5'TTAAACCCTAATTCCATGAT 3'
Utilize TAKARA company Primerstar GXL archaeal dna polymerase to expand, specifically comprise the following steps that
Reaction condition is as follows: 94 DEG C, 4min；98 DEG C, 10Sec；55 DEG C, 15Sec；68 DEG C, l.5min；72 DEG C, 10min；
30 circulations.OMEGA DNA purification kit is used PCR primer purification, PCR primer after purification to be carried with pEASY-Blunt
Body connects (the pEASY-Blunt Vector Cloning Kit test kit that this process uses TransGEN), and reaction system is such as
Under: cDNA fragment 4ul of IbERF5 gene, pEASY-Blunt carrier 1 μ L.Reaction condition: 20-30 DEG C, 30min.Connect product
Transformed E-Coli.DH5 α competence, is coated on IPTG, 100mg/ of X-Gal, 16ul 50mg/ml containing 40ul 25mg/ml
Cultivate on the LB Agar Plating of mL Amp, form single bacterium colony.Select white colony, use bacterium colony PCR method to confirm
The length scale of Insert Fragment in pEASY-Blunt carrier is consistent with expection.The order-checking of Nanjing Jin Sirui biotechnology company is sent to obtain
Obtain gene order SEQ ID NO.1.
Embodiment 2
IbERF5 protein sequence homogeneous assays, specific as follows:
Order-checking obtains cDNA total length 1235bp, and ORF is 705bp, encodes 234 aminoacid SEQ ID NO.2, translation is obtained
Obtain protein sequence to compare (http://blast.ncbi.nlm.nih.gov/Blast.cgi) with NCBI protein data, obtain
Obtained the plant species homologous genes similar to IbERF5 protein sequence.On the basis of multiple comparisons is analyzed, establish each same
The systematic evolution tree of source plant species gene, refers to Fig. 1.Including Fructus Capsici (Capsicum annuum, CaEREBP-C4), Fructus Lycopersici esculenti
(Solanum lycopersicum, SlERF5), Radix Dauci Sativae (Daucus carota, DcERF1), petunia (Petunia
Hybrida, PhEREBF12), Herba Artemisiae annuae (Artemisia annua), arabidopsis (Arabidopsis thaliana).Utilize
MEGA 5.1 software carries out the structure of systematic evolution tree, obtains IbERF5 sibship nearer with Fructus Capsici and Fructus Lycopersici esculenti sibship.
Embodiment 3
IbERF5 expression (as shown in Figure 2) after arid and salt stress, specific as follows:
The Seedling of clip Soviet Union No. 16 25cm of potato, and retain 3 fully expanded leaves, put into 25 DEG C of trainings in 1/2Hogland nutritional solution
Support 15 days, process 12h, 24h, 48h with 20%PEG6000, take 3 repetitions, after liquid nitrogen flash freezer, be stored in-80 DEG C of refrigerators.Extract RNA
And it being inverted to cDNA, method is as described in Example 1.Employing is the fluorescence quantitative kit of TOYOBO company.Reaction is at quantitative PCR
Carry out on instrument (Applied Biosystems stepone plus), detect the expression of gene according to the method for relative quantification,
The workbook that response procedures provides according to TOYOBO is carried out, and Rhizoma Dioscoreae esculentae Tubulin gene is as the internal reference in reaction, Tubulin
Primer sequence:
F, CAACTACCAGCCACCAACTGT, R, CAAGATCCTCACGAGCTTCAC；
The quantitative primer of IbERF5:
F, TTAAGCTGAGAGGCAGCAAA, R, CTTAATCACCGCCACTTCCT；And this gene after arid, salt stress
Expressed by Salt treatment.
Embodiment 4
The structure of binary plant expression vector pCAMBIA1305-IbERF5, specific as follows:
PCAMBIA1305-2 × 35s-IbERF5 carrier schematic diagram is as it is shown on figure 3, first with pEASY-Blunt-IbERF5
Plasmid is template, uses primer
IbERF5-PstI(F):5'aaCTGCAG ATGGCTTTATCTGAGGAAAG 3'
IbERF5-BamHI(R):5'ccGGATCC TTAAACCCTAATTCCATGAT 3'
Institute in restriction enzyme site PstI and BamHI, its reaction system and condition such as embodiment 1 is introduced respectively before and after IbERF5
State.Then PCR primer and pCAMBIA1305 empty carrier plasmid are respectively with BamHI and PstI double digestion, by the digestion products of the two
Connecting, linked system is as follows:
Connect product Transformed E-Coli.DH5 α, coat the concentration card containing 100mg/ml receive chloramphenicol resistance LB flat board on.37
DEG C cultivating, after 12h, picking list bacterium colony carries out bacterium colony PCR checking, by bacterium positive for bacterium colony PCR checking, shakes bacterium and extracts plasmid, enzyme action
Qualification obtains purpose band, finally send Jin Sirui order-checking company order-checking, result shows carrier pCAMBIA1305-2 × 35s-
IbERF5 builds correct.
Embodiment 5
For the structure of the Agrobacterium strain EHA105:pCAMBIA1305-2 × 35s-IbERF5 of plant transgene, specifically
As follows:
The agrobacterium strains that the present invention uses is EHA105.Use frozen-thawed method to be turned by the expression vector built
Enter Agrobacterium.Detailed process is: 1) ice bath melted EHA105 competent cell, adds at least 100ng and reclaims the expression load of purification
Body constitution grain, mixes gently, ice bath 20～30min；2) liquid nitrogen flash freezer 5min, 37 DEG C of thermal shock 5min, be immediately placed on ice 1～
2min；3) the LB culture medium of 800 μ L antibiotic-frees is added, 28 DEG C, 200rpm recovery 3.5h；4) 4000rpm is centrifuged 3min, sops up
Culture medium；5) mixing residue bacterium solution, be applied in add 100mg/ml card receive mycin and 100mg/ml rifampicin solid LB training base
On；6) it is inverted cultivation 30～48h for 28 DEG C；7) PCR detects positive colony, and 4 DEG C save backup.
Embodiment 6
IbERF5 converts Col wildtype Arabidopsis thaliana, specific as follows:
Positives for embodiment 5 clone is inoculated into the training of 50ml YEP (containing 100 μ g/ml Rif, 100 μ g/ml Kan) liquid
Supporting in base, 28 DEG C of 180rpm continue to cultivate to OD600=0.8.4000rpm is centrifuged 10min, abandons culture medium, collects thalline.By plan
South mustard infiltration buffer, by mycelium dilution OD600=0.6, is prepared as arabidopsis and infects liquid.As arabidopsis bolting 4-5cm
Preparation is infected, and the front 3d infected removes its terminal inflorescence, to utilize the growth of axillary inflorescence.After growing axillary inflorescence, under it
The flowers are in blossom in portion begins can convert during pollination.Water in a large number before conversion, and the alabastrum pollinated and pod are extractd.Preparation altogether
50ml arabidopsis infects liquid (1/2MS, 5% sucrose, 0.05%SilwetL-77), pours culture dish into, is soaked by the inflorescence of arabidopsis
Enter wherein 30s, notice that lotus throne leaf is not infected with bacterium solution.Take out plant, be disposed across in pallet, dark treatment 24h.The most uprightly put
Put square plate normal illumination to cultivate.When fruit pod maturation, results T0 is for seed.
By T0 for before planting seed at 4 DEG C of refrigerator vernalization 3d, after 0.5%NaClO surface sterilization, be seeded in 1/2MS solid
In culture medium, (a great number of elements, trace element halve, containing 20mg/L hygromycin) is cultivated.Select transformant after 7～14d, there is tide
The transformed plant physical ability of chloramphenicol resistance is growing containing in hygromycin culture medium, and root substantially extends, rather than transformant yellow gradually
Dead.When plant to be planted grows 4-5 sheet leaf, extract leaf DNA and carry out PCR qualification, primer and PCR program with in embodiment 1
The clone of segment in the middle of IbERF5 gene.1% agarose gel electrophoresis detection PCR primer.Cross PCR amplification and can obtain purpose sheet
Disconnected for arabidopsis positive transformant, is continued cultivation and treated that fruit pod maturation gathers in the crops T1 for seed.PCR is accredited as planting of the positive
The T1 that strain is gathered in the crops continues sowing for seed, also passes through hygromycin resistance screening, adds up its trait segregation ratio.Through X 2 test
Meet 3:1 segregation ratio in Mendel's law of inheritance.Partial resistance Seedling is implanted in soil, the transgenic kind that results T2 generation isozygotys
Son.
(1) identify turning IbERF5 gene arabidopsis drought resistance
Take IbERF5 process LAN arabidopsis strain (OV-1, OV-2) and wild type (WT) the plant point for converting is sowed at 1/
In 2MS culture medium one week, it is transplanted to Seedling be filled to of the same size moulding containing after Vermiculitum and Nutrition Soil 2:1 (v/v) mix homogeneously
Compost weight is consistent in material culturing pot and in each alms bowl of holding of weighing, and alms bowl face level size are consistent.Stop after 4 weeks watering
Observing phenotype after carrying out drought stress, one week, rehydration observes phenotype (see Fig. 4) in one day subsequently.Can draw, in arabidopsis
Process LAN IbERF5 can be with the response to arid of the positive regulation arabidopsis.
(2) identify turning IbERF5 gene arabidopsis salt-resistance
Take IbERF5 process LAN arabidopsis strain (OV-1, OV-2) and wild type (WT) the plant point for converting is sowed at 1/
In 2MS culture medium one week, it is transplanted to Seedling be filled to of the same size moulding containing after Vermiculitum and Nutrition Soil 2:1 (v/v) mix homogeneously
Compost weight is consistent in material culturing pot and in each alms bowl of holding of weighing, and alms bowl face level size are consistent.After 4 weeks, saturated water
By the NaCl submerging treatment of 300mM after water, observe phenotype after 10 days, and measure relative chlorophyll content (see Fig. 5).Can obtain
Going out, in arabidopsis, process LAN IbERF5 can be with the response to salt of the positive regulation arabidopsis.
Claims (7)
1. the IbERF5 gene of a coding sweet potato ERF transcription, it is characterised in that described IbERF5 gene is SEQ ID
Nucleotide sequence shown in NO.1.
The IbERF5 gene of coding sweet potato ERF transcription the most according to claim 1, it is characterised in that described
IbERF5 gene has enhancing plant salt endurance and the function of drought tolerance.
3. the protein of IbERF5 gene code described in claim 1, it is characterised in that described protein is SEQ ID No.2
Shown aminoacid sequence.
4. the expression vector pCAMBIA1305-2 × 35s-IbERF5 containing gene described in claim 1.
5. the Agrobacterium host cell EHA105:pCAMBIA1305-2 × 35s-containing expression vector described in claim 4
The structure of IbERF5.
6. the application in converting plant acquisition transfer-gen plant of the expression vector described in claim 4 or 5.
Application the most according to claim 6, it is characterised in that described plant is arabidopsis.
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| CN110295176A (en) * | 2019-07-23 | 2019-10-01 | 东北林业大学 | The polypeptide of poplar PsnERF1 gene cDNA and its coding |
| CN114085276A (en) * | 2021-11-17 | 2022-02-25 | 广东省科学院南繁种业研究所 | Upstream regulatory factor IbERF10 and application thereof in regulation of expression of purple sweet potato IbbHLH2 |
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| CN114395566A (en) * | 2022-03-28 | 2022-04-26 | 江苏省农业科学院 | Application of sweet potato ERF transcription factor IbERF4 in promoting synthesis of plant chlorogenic acid substances |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009035547A2 (en) * | 2007-09-07 | 2009-03-19 | University Of Virginia Patent Foundation | Compositions and related methods for modulating alkaloid production by controlling pmt promoter activation mediated by transcriptional factors erf and myc |
| CN102876674A (en) * | 2011-07-15 | 2013-01-16 | 长江大学 | Maize flooding stress response zmERF5 gene promoter |
| WO2013037959A1 (en) * | 2011-09-16 | 2013-03-21 | Vib Vzw | Methods and means to produce abiotic stress tolerant plants |
| CN104862320A (en) * | 2015-04-28 | 2015-08-26 | 江苏省农业科学院 | IbERF4 gene for coding sweet potato ERF transcription factor and application thereof |
| CN105367644A (en) * | 2015-12-23 | 2016-03-02 | 江苏省农业科学院 | Plant stress tolerance associated transcription factor, encoding gene of plant stress tolerance associated transcription factor, and application of encoding gene |
| CN105671058A (en) * | 2016-03-24 | 2016-06-15 | 江苏省农业科学院 | Gene for encoding sweet potato ERF (ethylene responsive factor) transcription factor |
-
2016
- 2016-08-11 CN CN201610659167.8A patent/CN106222182B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009035547A2 (en) * | 2007-09-07 | 2009-03-19 | University Of Virginia Patent Foundation | Compositions and related methods for modulating alkaloid production by controlling pmt promoter activation mediated by transcriptional factors erf and myc |
| CN102876674A (en) * | 2011-07-15 | 2013-01-16 | 长江大学 | Maize flooding stress response zmERF5 gene promoter |
| WO2013037959A1 (en) * | 2011-09-16 | 2013-03-21 | Vib Vzw | Methods and means to produce abiotic stress tolerant plants |
| CN104862320A (en) * | 2015-04-28 | 2015-08-26 | 江苏省农业科学院 | IbERF4 gene for coding sweet potato ERF transcription factor and application thereof |
| CN105367644A (en) * | 2015-12-23 | 2016-03-02 | 江苏省农业科学院 | Plant stress tolerance associated transcription factor, encoding gene of plant stress tolerance associated transcription factor, and application of encoding gene |
| CN105671058A (en) * | 2016-03-24 | 2016-06-15 | 江苏省农业科学院 | Gene for encoding sweet potato ERF (ethylene responsive factor) transcription factor |
Non-Patent Citations (2)
| Title |
|---|
| LIU. J等: "ethylene-responsive-element-binding factor 12", 《GENBANK DATABASE》 * |
| 边小峰等: "甘薯抗逆相关基因IbERF3的克隆与表达分析", 《华北农学报》 * |
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| CN110295176A (en) * | 2019-07-23 | 2019-10-01 | 东北林业大学 | The polypeptide of poplar PsnERF1 gene cDNA and its coding |
| CN114085276A (en) * | 2021-11-17 | 2022-02-25 | 广东省科学院南繁种业研究所 | Upstream regulatory factor IbERF10 and application thereof in regulation of expression of purple sweet potato IbbHLH2 |
| CN114085276B (en) * | 2021-11-17 | 2022-09-16 | 广东省科学院南繁种业研究所 | Upstream regulatory factor IbERF10 and application thereof in regulation and control of IbbHLH2 expression of purple sweet potato |
| CN114134158A (en) * | 2021-12-17 | 2022-03-04 | 广东省科学院南繁种业研究所 | IbDRM gene of purple sweet potato and application thereof |
| CN115927361A (en) * | 2022-03-18 | 2023-04-07 | 华中农业大学 | Gene TgFTS1 related to flowering control and petal senescence of bulbous flowers and application thereof |
| CN115927361B (en) * | 2022-03-18 | 2024-05-24 | 华中农业大学 | Gene TgFTS1 related to flowering regulation and petal aging of bulbous flower and application thereof |
| CN114395566A (en) * | 2022-03-28 | 2022-04-26 | 江苏省农业科学院 | Application of sweet potato ERF transcription factor IbERF4 in promoting synthesis of plant chlorogenic acid substances |
| CN114395566B (en) * | 2022-03-28 | 2022-05-24 | 江苏省农业科学院 | Application of sweet potato ERF transcription factor IbERF4 in promoting synthesis of plant chlorogenic acid substances |
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