CN106220728A - A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid - Google Patents
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid Download PDFInfo
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- CN106220728A CN106220728A CN201610705951.8A CN201610705951A CN106220728A CN 106220728 A CN106220728 A CN 106220728A CN 201610705951 A CN201610705951 A CN 201610705951A CN 106220728 A CN106220728 A CN 106220728A
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- 241000206588 Gracilaria tenuistipitata Species 0.000 title claims abstract description 46
- 239000007788 liquid Substances 0.000 title claims abstract description 45
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 title claims abstract description 34
- 239000010447 natron Substances 0.000 title claims abstract description 34
- 238000005516 engineering process Methods 0.000 title claims abstract description 23
- 239000012528 membrane Substances 0.000 claims abstract description 37
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 30
- 238000000746 purification Methods 0.000 claims abstract description 23
- 239000000047 product Substances 0.000 claims abstract description 22
- 239000011265 semifinished product Substances 0.000 claims abstract description 20
- 239000006228 supernatant Substances 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000000284 extract Substances 0.000 claims abstract description 12
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229920002271 DEAE-Sepharose Polymers 0.000 claims abstract description 10
- 239000012505 Superdex™ Substances 0.000 claims abstract description 10
- 239000003513 alkali Substances 0.000 claims abstract description 10
- 239000012141 concentrate Substances 0.000 claims abstract description 10
- 238000007667 floating Methods 0.000 claims abstract description 10
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims abstract description 10
- 239000012535 impurity Substances 0.000 claims abstract description 10
- 238000005342 ion exchange Methods 0.000 claims abstract description 10
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 229920001817 Agar Polymers 0.000 claims description 21
- 239000008272 agar Substances 0.000 claims description 21
- 238000004519 manufacturing process Methods 0.000 claims description 19
- 238000002203 pretreatment Methods 0.000 claims description 19
- 229920002492 poly(sulfone) Polymers 0.000 claims description 18
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 9
- 239000002244 precipitate Substances 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 238000000605 extraction Methods 0.000 abstract description 16
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 239000013543 active substance Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to the isolated and purified field of phycobniliprotein, it is specifically related to a kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, described Technology comprises the following steps: (1) alkali liquor is collected: the impurity in natron liquid and float are precipitated, again except floating thing, extraction supernatant is standby；(2) concentrate: supernatant is prepared, by membrane filter method, the concentrated solution that protein content is 0.5%～2.0%；(3) ultrafiltration: use NaNO3Height oozes the phycobniliprotein in ultrafiltration separating-purifying concentrated solution, obtains phycobniliprotein semifinished product；(4) isolated and purified: phycobniliprotein semifinished product first to be used DEAE Sepharose ion exchange column purification, then is further purified through SephacrylS 300HR post, obtain phycobniliprotein first product；Phycobniliprotein first product is obtained phycobniliprotein through hydroxyapatite chromatography post, Superdex 75 column purification successively, the present invention effectively extracts highly purified phycobniliprotein from Gracilaria tenuistipitata dish deep processing natron liquid, technique is simple simultaneously, and yield is high, can obtain significant economic benefit.
Description
Technical field
The present invention relates to the isolated and purified field of phycobniliprotein, be specifically related to a kind of from the separation of Gracilaria tenuistipitata dish deep processing natron liquid
The Technology of purification phycobniliprotein.
Background technology
Gracilaria tenuistipitata dish contains abundant active substance, such as functions such as phycobniliprotein, highly unsaturated fatty acid and agar polysaccharide
Active component, especially phycobniliprotein and agar polysaccharide so that Gracilaria tenuistipitata dish has multiple important physiological function, can extensively apply
In fields such as food, medicine and cosmetics.Comprehensive intensification to Gracilaria tenuistipitata research processing experience the most both at home and abroad, the shortest,
The active substance obtaining high yield on the premise of low cost has become as the Main way of research.
Wherein phycobniliprotein not only has good light characteristic of catching, and has good fluorescent characteristic, nourishing healthy merit
Energy and medical value.Its purposes is extremely wide, can be applied to the industry such as food, cosmetics as natural pigment, can be made into again glimmering
Light reagent, in the field of immunology and clinical diagnose.Meanwhile, phycobniliprotein also have antioxidation, antitumor and
Improve the important physiological function such as immunity of organisms, be the valuable source of health product and medicine etc..
In the production and processing of agar product, mainly with ocean near-coastal resources red algae Gracilaria tenuistipitata dish as raw material, through analyzing algae
Body contains the phycobniliprotein of about 6%, in the agar production course of processing, uses substantial amounts of sig water that frond is carried out front place
Reason, the phycobniliprotein of part is dissolved in sig water in the process, and discharges together with other waste water, not only causes waste but also cause
Environmental pollution, therefore, works out the Technology tool extracting phycobniliprotein in the pre-treatment sig water from agar production process
There are far-reaching social meaning and economic worth.
Summary of the invention
The invention provides a kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, effectively
Extracting highly purified phycobniliprotein from Gracilaria tenuistipitata dish deep processing natron liquid, technique is simple simultaneously, and yield is high, can obtain significant warp
Ji benefit.
For realizing object above, the present invention is achieved by the following technical programs:
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, comprises the following steps:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution
Chi Zhong, stands 12～24h, after the impurity in solution, float precipitate completely, removes the floating thing of liquid level, in extraction
Clear liquid is placed in container, standby；
(2) concentrate: by membrane filter method, above-mentioned supernatant is removed a certain amount of sig water, and obtaining protein content is
The concentrated solution of 0.5%～2.0%, and reclaim removed sig water；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little
Molecule protein, obtains phycobniliprotein semifinished product；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Preferably, in described step (2), the protein content of concentrated solution is 0.8%～1.8%.
Preferably, in described step (2), the protein content of concentrated solution is 1.2%～1.6%.
Preferably, the sig water reclaimed in described step (2) is for Gracilaria tenuistipitata dish pre-treatment.
Preferably, in described step (3), polysulfone membrane selected by filter membrane during ultrafiltration.
Preferably, the retaining molecular weight of described polysulfone membrane is 5000～6000.
The invention have the benefit that the present invention first uses NaNO3Height oozes-ultrafiltration separating-purifying phycobniliprotein, safety
Nontoxic, use multiple purification technique to be used in mixed way afterwards, substantially increase purification efficiency and the purity of phycobniliprotein, in the present invention
Utilize Gracilaria tenuistipitata dish deep processing natron liquid containing rich from the Technology of the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid
The feature of rich phycobniliprotein, the isolated and purified system of reasonable construction, effectively extract high-purity from Gracilaria tenuistipitata dish deep processing natron liquid
The phycobniliprotein of degree, technique is simple simultaneously, and yield is high, and can realize the cycling and reutilization of sig water, economizes in raw materials, and improves resource
Utilization ratio, can obtain significant economic benefit, and effectively prevent environmental pollution.
Detailed description of the invention
Further illustrate the technical solution of the present invention below in conjunction with specific embodiment, embodiment is not to be construed as right
The restriction of technical solution.
Embodiment 1:
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, comprises the following steps:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution
Chi Zhong, stands 24h, and impurity, float in solution precipitate completely, removes the floating thing of liquid level, and extraction supernatant is placed in
In container, standby；
(2) concentrate: by membrane filter method, above-mentioned supernatant is removed a certain amount of sig water, and obtaining protein content is
The concentrated solution of 0.5%, and reclaim removed sig water, use it for the pre-treatment of Gracilaria tenuistipitata dish in agar production process；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little
After molecule protein, obtaining phycobniliprotein semifinished product, wherein filter membrane during ultrafiltration selects polysulfone membrane, this polysulfone membrane retaining molecular weight
It is 5000；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Testing result shows: phycobniliprotein extraction ratio is 84.5%.
Embodiment 2:
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, comprises the following steps:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution
Chi Zhong, stands 12h, and impurity, float in solution precipitate completely, removes the floating thing of liquid level, and extraction supernatant is placed in
In container, standby；
(2) concentrate: by membrane filter method, above-mentioned supernatant is removed a certain amount of sig water, and obtaining protein content is
The concentrated solution of 1.8%, and reclaim removed sig water, use it for the pre-treatment of Gracilaria tenuistipitata dish in agar production process；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little
After molecule protein, obtaining phycobniliprotein semifinished product, wherein filter membrane during ultrafiltration selects polysulfone membrane, this polysulfone membrane retaining molecular weight
It is 5000；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Testing result shows: phycobniliprotein extraction ratio is 85.2%.
Embodiment 3:
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, comprises the following steps:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution
Chi Zhong, stands 16h, and impurity, float in solution precipitate completely, removes the floating thing of liquid level, and extraction supernatant is placed in
In container, standby；
(2) concentrate: by membrane filter method, above-mentioned supernatant is removed a certain amount of sig water, and obtaining protein content is
The concentrated solution of 1.2%, and reclaim removed sig water, use it for the pre-treatment of Gracilaria tenuistipitata dish in agar production process；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little
After molecule protein, obtaining phycobniliprotein semifinished product, wherein filter membrane during ultrafiltration selects polysulfone membrane, this polysulfone membrane retaining molecular weight
It is 5000；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Testing result shows: the extraction ratio of phycobniliprotein is 85.4%.
Embodiment 4:
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, comprises the following steps:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution
Chi Zhong, stands 18h, and impurity, float in solution precipitate completely, removes the floating thing of liquid level, and extraction supernatant is placed in
In container, standby；
(2) concentrate: by membrane filter method, above-mentioned supernatant is removed a certain amount of sig water, and obtaining protein content is
The concentrated solution of 2.0%, and reclaim removed sig water, use it for the pre-treatment of Gracilaria tenuistipitata dish in agar production process；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little
After molecule protein, obtaining phycobniliprotein semifinished product, wherein filter membrane during ultrafiltration selects polysulfone membrane, this polysulfone membrane retaining molecular weight
It is 6000；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Testing result shows: phycobniliprotein extraction ratio is 84.2%.
Embodiment 5:
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, comprises the following steps:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution
Chi Zhong, stands 20h, and impurity, float in solution precipitate completely, removes the floating thing of liquid level, and extraction supernatant is placed in
In container, standby；
(2) concentrate: by membrane filter method, above-mentioned supernatant is removed a certain amount of sig water, and obtaining protein content is
The concentrated solution of 0.8%, and reclaim removed sig water, use it for the pre-treatment of Gracilaria tenuistipitata dish in agar production process；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little
After molecule protein, obtaining phycobniliprotein semifinished product, wherein filter membrane during ultrafiltration selects polysulfone membrane, this polysulfone membrane retaining molecular weight
It is 5000；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Testing result shows: the extraction ratio of phycobniliprotein is 84.8%.
Embodiment 6:
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, comprises the following steps:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution
Chi Zhong, stands 14h, and impurity, float in solution precipitate completely, removes the floating thing of liquid level, and extraction supernatant is placed in
In container, standby；
(2) concentrate: by membrane filter method, above-mentioned supernatant is removed a certain amount of sig water, and obtaining protein content is
The concentrated solution of 1.4%, and reclaim removed sig water, use it for the pre-treatment of Gracilaria tenuistipitata dish in agar production process；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little
After molecule protein, obtaining phycobniliprotein semifinished product, wherein filter membrane during ultrafiltration selects polysulfone membrane, this polysulfone membrane retaining molecular weight
It is 6000；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Testing result shows: the extraction ratio of phycobniliprotein is 85.8%.
Embodiment 7:
A kind of Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, comprises the following steps:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution
Chi Zhong, stands 22h, and impurity, float in solution precipitate completely, removes the floating thing of liquid level, and extraction supernatant is placed in
In container, standby；
(2) concentrate: by membrane filter method, above-mentioned supernatant is removed a certain amount of sig water, and obtaining protein content is
The concentrated solution of 1.6%, and reclaim removed sig water, use it for the pre-treatment of Gracilaria tenuistipitata dish in agar production process；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little
After molecule protein, obtaining phycobniliprotein semifinished product, wherein filter membrane during ultrafiltration selects polysulfone membrane, this polysulfone membrane retaining molecular weight
It is 6000；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Testing result shows: the extraction ratio of phycobniliprotein is 85.1%.
It is more than the description to the embodiment of the present invention, by the described above to the disclosed embodiments, makes this area special
Industry technical staff is capable of or uses the present invention.Those skilled in the art are come by the multiple amendment to these embodiments
Saying and will be apparent from, generic principles defined herein can be in the situation without departing from the spirit or scope of the present invention
Under, realize in other embodiments.Therefore, the present invention is not intended to be limited to the embodiments shown herein, and is intended to symbol
Close the widest scope consistent with principles disclosed herein and features of novelty.
Claims (6)
1. the Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid, it is characterised in that include following
Step:
(1) alkali liquor is collected: in agar production process, and the natron liquid after Gracilaria tenuistipitata dish carrying out pre-treatment extracts to solution pool
In, stand 12～24h, after the impurity in solution, float precipitate completely, remove the floating thing of liquid level, extract supernatant
Liquid is placed in container, standby；
(2) concentrate: above-mentioned supernatant is removed a certain amount of sig water by membrane filter method, obtain protein content be 0.5%～
The concentrated solution of 2.0%, and reclaim removed sig water；
(3) ultrafiltration: use NaNO3Height oozes-ultrafiltration separating-purifying concentrated solution in phycobniliprotein, remove inorganic salt and little molecule
Protein, obtains phycobniliprotein semifinished product；
(4) isolated and purified: phycobniliprotein semifinished product is first used DEAE-Sepharose ion exchange column purification, then warp
SephacrylS-300HR post is further purified, and obtains phycobniliprotein first product；By phycobniliprotein first product successively through hydroxyapatite
Chromatographic column, Superdex 75 column purification obtain phycobniliprotein.
Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid the most according to claim 1, it is special
Levying and be, in described step (2), the protein content of concentrated solution is 0.8%～1.8%.
Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid the most according to claim 2, it is special
Levying and be, in described step (2), the protein content of concentrated solution is 1.2%～1.6%.
Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid the most according to claim 1, it is special
Levying and be, the sig water reclaimed in described step (2) is for Gracilaria tenuistipitata dish pre-treatment.
Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid the most according to claim 1, it is special
Levying and be, in described step (3), polysulfone membrane selected by filter membrane during ultrafiltration.
Technology from the Gracilaria tenuistipitata dish deep processing isolated and purified phycobniliprotein of natron liquid the most according to claim 5, it is special
Levying and be, the retaining molecular weight of described polysulfone membrane is 5000～6000.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114760849A (en) * | 2019-07-30 | 2022-07-15 | 特洛弗克有限责任公司 | Plant-based food |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101003565A (en) * | 2006-12-27 | 2007-07-25 | 山东理工大学 | Method for preparing phycocyanin and allophycocyanin at same time |
| CN101519425A (en) * | 2008-02-26 | 2009-09-02 | 上海水产大学 | Method for separating and purifying high-purity phycobiliprotein from spirulina |
| CN104292327A (en) * | 2014-09-28 | 2015-01-21 | 北京林业大学 | Method for extracting phycobiliprotein from spirulina |
| CN105237614A (en) * | 2015-09-09 | 2016-01-13 | 湖北民族学院 | Method of extracting bio-active substances from algae |
| CN105271576A (en) * | 2015-11-10 | 2016-01-27 | 中国科学院海洋研究所 | Wastewater treatment method in agar production |
-
2016
- 2016-08-23 CN CN201610705951.8A patent/CN106220728A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101003565A (en) * | 2006-12-27 | 2007-07-25 | 山东理工大学 | Method for preparing phycocyanin and allophycocyanin at same time |
| CN101519425A (en) * | 2008-02-26 | 2009-09-02 | 上海水产大学 | Method for separating and purifying high-purity phycobiliprotein from spirulina |
| CN104292327A (en) * | 2014-09-28 | 2015-01-21 | 北京林业大学 | Method for extracting phycobiliprotein from spirulina |
| CN105237614A (en) * | 2015-09-09 | 2016-01-13 | 湖北民族学院 | Method of extracting bio-active substances from algae |
| CN105271576A (en) * | 2015-11-10 | 2016-01-27 | 中国科学院海洋研究所 | Wastewater treatment method in agar production |
Non-Patent Citations (4)
| Title |
|---|
| 刘名求;杨贤庆;戚勃;邓建朝;宋莹;: "江蓠活性多糖与藻胆蛋白的研究现状与展望", 食品工业科技, no. 13, pages 1 * |
| 李华佳等: "藻胆蛋白的提取技术与生物活性研究", 食品科学, vol. 26, no. 7, pages 1 * |
| 谷洋洋;刘冰;杜虹;张允允;秦松;: "高纯度藻胆蛋白的分离纯化研究进展", 海洋科学, no. 07, pages 170 - 177 * |
| 陈美珍, 张永雨, 余杰, 谢雄彬: "龙须菜藻胆蛋白的分离及其清除自由基作用的初步研究", 食品科学, no. 03, pages 159 - 162 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114760849A (en) * | 2019-07-30 | 2022-07-15 | 特洛弗克有限责任公司 | Plant-based food |
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