CN113388026B - Method for synchronously extracting phycocyanin and algae oil from spirulina - Google Patents
Method for synchronously extracting phycocyanin and algae oil from spirulina Download PDFInfo
- Publication number
- CN113388026B CN113388026B CN202110885024.XA CN202110885024A CN113388026B CN 113388026 B CN113388026 B CN 113388026B CN 202110885024 A CN202110885024 A CN 202110885024A CN 113388026 B CN113388026 B CN 113388026B
- Authority
- CN
- China
- Prior art keywords
- spirulina
- phycocyanin
- phase
- temperature
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108010053210 Phycocyanin Proteins 0.000 title claims abstract description 75
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 64
- 235000016425 Arthrospira platensis Nutrition 0.000 title claims abstract description 54
- 240000002900 Arthrospira platensis Species 0.000 title claims abstract description 54
- 229940082787 spirulina Drugs 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 52
- 229920000642 polymer Polymers 0.000 claims abstract description 64
- 238000000605 extraction Methods 0.000 claims abstract description 60
- 239000000243 solution Substances 0.000 claims abstract description 33
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 24
- 239000000843 powder Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000011780 sodium chloride Substances 0.000 claims abstract description 10
- 239000007864 aqueous solution Substances 0.000 claims abstract description 9
- 238000007710 freezing Methods 0.000 claims abstract description 7
- 230000008014 freezing Effects 0.000 claims abstract description 7
- 238000000502 dialysis Methods 0.000 claims abstract description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 5
- 229920001223 polyethylene glycol Polymers 0.000 claims description 10
- 239000002202 Polyethylene glycol Substances 0.000 claims description 9
- 230000002209 hydrophobic effect Effects 0.000 claims description 9
- -1 polyethylene Polymers 0.000 claims description 9
- 239000004698 Polyethylene Substances 0.000 claims description 8
- 239000004743 Polypropylene Substances 0.000 claims description 8
- 238000005191 phase separation Methods 0.000 claims description 8
- 229920000573 polyethylene Polymers 0.000 claims description 8
- 229920001155 polypropylene Polymers 0.000 claims description 8
- 238000001764 infiltration Methods 0.000 claims description 4
- 230000008595 infiltration Effects 0.000 claims description 4
- 239000012266 salt solution Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000005470 impregnation Methods 0.000 claims description 2
- 230000010355 oscillation Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 6
- 230000001360 synchronised effect Effects 0.000 abstract description 6
- 238000005265 energy consumption Methods 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 238000002791 soaking Methods 0.000 abstract description 2
- 239000012071 phase Substances 0.000 description 37
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 239000008346 aqueous phase Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000003960 organic solvent Substances 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- IDOQDZANRZQBTP-UHFFFAOYSA-N 2-[2-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=CC=C1OCCO IDOQDZANRZQBTP-UHFFFAOYSA-N 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 229920004929 Triton X-114 Polymers 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000005185 salting out Methods 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 238000000194 supercritical-fluid extraction Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229960000502 poloxamer Drugs 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/106—Production of fats or fatty oils from raw materials by extracting using ultra-sounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/108—Production of fats or fatty oils from raw materials by extracting after-treatment, e.g. of miscellae
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110885024.XA CN113388026B (en) | 2021-08-03 | 2021-08-03 | Method for synchronously extracting phycocyanin and algae oil from spirulina |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110885024.XA CN113388026B (en) | 2021-08-03 | 2021-08-03 | Method for synchronously extracting phycocyanin and algae oil from spirulina |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113388026A CN113388026A (en) | 2021-09-14 |
CN113388026B true CN113388026B (en) | 2022-09-27 |
Family
ID=77622342
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110885024.XA Active CN113388026B (en) | 2021-08-03 | 2021-08-03 | Method for synchronously extracting phycocyanin and algae oil from spirulina |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113388026B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116199770B (en) * | 2022-11-24 | 2024-03-26 | 盐池县怡健生物工程有限公司 | Method for extracting and purifying phycocyanin from spirulina platensis |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010128007A1 (en) * | 2009-05-06 | 2010-11-11 | Technische Universiteit Eindhoven | Self-healing polymers |
SI3318571T1 (en) * | 2011-03-16 | 2021-08-31 | F. Hoffmann-La Roche Ag | Ion exchange chromatography with improved selectivity for the separation of polypeptide monomers, aggregates and fragments by modulation of the mobile phase |
AU2013318647B2 (en) * | 2012-09-21 | 2017-10-26 | Massachusetts Institute Of Technology | Micro-fluidic device and uses thereof |
CN106008705A (en) * | 2016-06-22 | 2016-10-12 | 瑞安市智造科技有限公司 | Method for separating and purifying phycocyanin by means of combination of two aqueous phase extraction and ultrasonic waves |
-
2021
- 2021-08-03 CN CN202110885024.XA patent/CN113388026B/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN113388026A (en) | 2021-09-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109810201B (en) | Ultrasonic wave composite acidic water extraction method for cordyceps polysaccharide and cordycepin in cordyceps militaris | |
CN103160482B (en) | Method for preparing egg white lysozyme and active protein by adopting coseparation | |
CN102488713B (en) | Method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrated solution | |
CN113388026B (en) | Method for synchronously extracting phycocyanin and algae oil from spirulina | |
CN104388178B (en) | A kind of method that DHA algal oil is extracted from alga cells | |
CN103102408B (en) | Method for extracting phycocyanin from spirulina | |
CN106008705A (en) | Method for separating and purifying phycocyanin by means of combination of two aqueous phase extraction and ultrasonic waves | |
CN106906199A (en) | A kind of fast purifying squid viscera albumen enzyme method and application | |
CN104592412A (en) | Method for extracting chestnut polysaccharide | |
CN112941128B (en) | Method for extracting tremella polysaccharide from tremella | |
CN113584108A (en) | Preparation method of spleen aminopeptide | |
CN104388179B (en) | It is a kind of that DHA algal oil and the method for algae protein are extracted from algae | |
CN111978383A (en) | Method for synergistically extracting phycoerythrin and grease from wet algae | |
CN101962635B (en) | Three-step two aqueous phase extraction method of ginger protease | |
CN104558228A (en) | Method for preparing sulphated polysaccharides from abalone | |
CN102515394B (en) | Method for pre-extracting and recovering polyphenol compounds in olive oil treatment wastewater | |
CN1488364A (en) | Method for treating aloe gel, aloe leaf gel and aloe hide glue and aloe polyaccharide obtained thereof | |
CN105622342A (en) | Method for separating 2,3-butanediol | |
CN115626958A (en) | Method for separating edible fungus polysaccharide based on silicon carbide ceramic membrane technology and application | |
CN108314702A (en) | A kind of method of inverse micelle abstraction-impulse electric field combined separation purifying protein | |
CN108409828A (en) | One kind method for extracting proteins from microalgae algae-residue | |
CN110257370B (en) | Method for rapidly extracting rockfish mitochondrial genome DNA | |
CN112480241A (en) | Method for extracting phycocyanin from fresh spirulina harvested in high-salt environment | |
CN113583145A (en) | Method for extracting polysaccharide based on tremella spore fermentation liquor | |
CN112574329A (en) | Method for preparing crude heparin sodium by using sow intestines through biological enzyme method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231101 Address after: 010000 Room 2037, 2nd Floor, Gengyun Building, Xinhua East Street, Saihan District, Hohhot City, Inner Mongolia Autonomous Region Patentee after: Inner Mongolia Keguoguo Technology Service Co.,Ltd. Address before: 341000 No. 86 Hongqi Avenue, Jiangxi, Ganzhou Patentee before: Jiangxi University of Science and Technology Patentee before: Jiangmen Pan-Asian Institute of Bioengineering and Health Effective date of registration: 20231101 Address after: 017000 Spirulina Industry Park E6, Ulan Town, Etuoke Banner, Ordos City, Inner Mongolia Autonomous Region Patentee after: Otog Banner Lvchuang Algae Industry Co.,Ltd. Address before: 010000 Room 2037, 2nd Floor, Gengyun Building, Xinhua East Street, Saihan District, Hohhot City, Inner Mongolia Autonomous Region Patentee before: Inner Mongolia Keguoguo Technology Service Co.,Ltd. |
|
TR01 | Transfer of patent right |