CN108409828A - One kind method for extracting proteins from microalgae algae-residue - Google Patents
One kind method for extracting proteins from microalgae algae-residue Download PDFInfo
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- CN108409828A CN108409828A CN201810488168.XA CN201810488168A CN108409828A CN 108409828 A CN108409828 A CN 108409828A CN 201810488168 A CN201810488168 A CN 201810488168A CN 108409828 A CN108409828 A CN 108409828A
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- algae
- residue
- microalgae
- protein
- extracting proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
Abstract
The invention discloses one kind from microalgae algae-residue method for extracting proteins, includes the following steps：1）Sodium hydroxide solution is added in algae-residue, is stirred heating extraction, to get alkali extract after separation of solid and liquid；2）By step 1）Obtained alkali extract is adjusted with acid pH to acidity, is precipitated to get thick protein after separation of solid and liquid；3）By step 2）Obtained crude protein precipitation redissolved successively, desalination and concentration, dry protein powder.Present invention process flow is reasonable, it is easy to operate, the algae-residue discarded after extraction pigment, grease, pigment grease and polysaccharide is made full use of, turns waste into wealth and increases economic benefit, to extend microalgae recovery link, the overall economy quality of microalgae biomass utilization is effectively increased.
Description
Technical field
The present invention relates to one kind from microalgae algae-residue method for extracting proteins, belongs to biotechnology.
Background technology
Microalgae is that one kind is widely distributed in land, ocean, full of nutrition, can carry out the unicellular life of photosynthetic autotrophy
Object containing abundant grease, pigment, protein, polysaccharide isoreactivity substance in cell, therefore can be widely applied to food, medicine
The fields such as product, cosmetics, feed.
Recent domestic increases the utilization to microalgae cell.Such as the entitled " microalgae greases of CN103849458B
Extracting method " patent of invention, use chloroform and ethyl alcohol volume ratio for 3:1 mixed organic solvents can be extracted effectively in microalgae
98% grease.Such as the patent of invention of CN1272305C entitled " a kind of bloom blue algae extraction-linolenic method ", using stone
Oily ether solvents are extracted gamma-Linolenic acid from bloom blue algae.Such as use dichloromethane high efficiency extraction lutein from chlorella
（Martina D E, Davide D F, Irini A. Novel protocol for lutein extraction from
microalga Chlorella vulgaris[J]. Biochemical Engineering Journal, 2017, 127:
175-179）.Such as the Chinese patent of CN105254551B entitled " method of rapid extraction astaxanthin from haematococcus pluvialis ",
Using the astaxanthin in oil-ethyl alcohol double solvents rapid extraction haematococcus pluvialis.As CN100537532C it is entitled " it is a kind of from
In Dunaliella salina extract carotenoid and edible glycerol method " patent of invention, using supercritical carbon dioxide method from
Carotenoid and edible glycerol are extracted in Dunaliella salina.Some researchs use supercritical CO2Extraction and subcritical normal butane
Extraction fromPhaeodactylum tricornutum、 Nannochloropsis oculataWithPorphyridium cruentumThree kinds of marine microalgaes are extracted ω -3 and omega 6 polyunsaturated fatty acid, carotenoid（Rafael F, Â
ngelo P M, Simone M, et al. Polyunsaturated ω-3 and ω-6 fatty acids, total
carotenoids and antioxidant activity of three marine microalgae extracts
obtained by supercritical CO2 and subcritical n-butane[J]. The Journal of
Supercritical Fluids, 2018, 133(1): 437-443）.Some researchers fromChlorellaSp. it is carried in
Polysaccharides（Song H, He M L, Gu C K, et al. Extraction Optimization, Purification,
Antioxidant Activity, and Preliminary Structural Characterization of Crude
Polysaccharide from an Arctic Chlorella sp. [J]. Polymers, 2018, 10(3): 292）.
However, with the rise of microalgae industry, a large amount of algae-residues are necessarily will produce after extracting these substances in microalgae, and relevant research
It was found that microalgae algae-residue is still important biomass resource, still contain in algae-residue more using component.Some are studied at present
Person has found the algae-residue Amino Acids in Proteins A wide selection of colours and designs after extraction grease, balanced proportion, it is necessary to which amino acid accounts for total amino acid content
44.3%, essential amino acid index, amino acid score, chemical score, biological value, nutrient index and the amino acid of protein
Ratio coefficient scoring is respectively 82.24,63.32,46.66,77.94,35.84 and 74.21, can be used as important albumen
Source has the potential for being developed into protein feed（Fan Xing, Huang Yanfei, Liao Yonghong wait scenedesmus algae-residue nutritional ingredients point
Analysis and protein extraction process optimization [J] process engineering journals, 2015,15 (1): 126-131.）.But the production of current microalgae
Industry chain is not yet ripe, and shortage efficiently uses technology to algae-residue, and high level high-qualityization for having not been achievable algae-residue utilizes process.
Invention content
In order to make up above-mentioned the deficiencies in the prior art, the present invention proposes to extract the side of protein in a kind of algae-residue from microalgae
Method can be extracted by algae-residue and prepare algae albumen.Using the extraction process, during microalgae biomass can be made full use of to develop and use
The a large amount of algae-residues generated realize that high level high-qualityization of algae-residue utilizes process, extend microalgae recovery link, to improve microalgae biomass
The overall economy quality of utilization provides a kind of new method for the comprehensive development and utilization of microalgae resource.
To achieve the above object, the present invention adopts the following technical scheme that：
One kind method for extracting proteins from microalgae algae-residue, includes the following steps：
1）Algae-residue protein alkalinity extraction：Sodium hydroxide solution is added in algae-residue, heating extraction is stirred, is separated by solid-liquid separation
Afterwards to get alkali extract；
2）Isoelectric points of proteins precipitates：By step 1）Obtained alkali extract is adjusted with acid pH to acidity, to get thick after separation of solid and liquid
Protein precipitation；
3）It is prepared by protein powder：By step 2）Obtained crude protein precipitation redissolved successively, desalination and concentration, dry protein
Powder.
The step 1）In, algae-residue is to have extracted any ingredient in phycochrome, algae oil fat, phycochrome grease and polysaccharides
Remaining part afterwards.
The step 1）In, the algae in algae-residue source is chlorella, Du Shi algaes, micro- quasi- ball algae, chlamydomonas, scenedesmus, phaeodactylum tricornutum
Refer to one kind in algae, purple ball algae, spirulina, chrysophyceae, hidden dinoflagellate, haematococcus pluvialis.
The step 1）In, naoh concentration 1wt%-30wt%, extraction conditions are：Solid-to-liquid ratio is 7.5-15:1 g/
L, temperature is 30-80 DEG C, extraction time is 10-60 min.
The step 1）With step 2）In, solid-liquid separating method is one kind in standing, centrifugation, filtering.
The step 2）In, acid is sulfuric acid or hydrochloric acid, adjusts pH to 3.5-5.5.
The step 3）In, desalination and concentration method is ultrafiltration or nanofiltration.
The step 4）In, drying means is one kind in heated-air drying, vacuum drying, freeze-drying, spray drying.
Using algae-residue protein powder made from said extracted method, protein content is 80wt% ~ 95wt%.
The remarkable advantage of the present invention is：The present invention takes full advantage of the characteristics of algae-residue constituent, to extract algae color
Algae-residue after element, algae oil fat, phycochrome grease and polysaccharides is raw material, extracts protein using alkali extraction and acid precipitation, further
Protein powder is prepared by desalination and concentration, drying and other steps, realizes that high level high-qualityization of algae-residue utilizes.This method technique stream simultaneously
Cheng Heli, it is easy to operate, algae-residue is turned waste into wealth and increases economic benefit, to improve the entirety of microalgae biomass utilization
Economy can provide a new way for the microalgae biomass development process of low cost.
Description of the drawings
Fig. 1 is microalgae algae-residue Protein Extraction flow chart.
Specific implementation mode
Technical scheme of the present invention is described with reference to specific embodiment.It is as described below to implement for the representative of the present invention
Example can be used for explaining and support the present invention, but do not made any form of restriction to the present invention, any without departing from the technology of the present invention
Plan content, and according to the technical essence of the invention to simple modification, equivalent change and modification made by following embodiment,
In the range of still falling within technical solution of the present invention.
Embodiment 1
One kind method for extracting proteins from microalgae algae-residue, includes the following steps：
1）Algae-residue protein alkalinity extraction：The chlorella that 750 g have carried phycochrome is added in 100 L 1wt% sodium hydroxide solutions
In algae-residue, agitating and heating extracts 60 min under the conditions of 30 DEG C, and reaction stands 3 h under the conditions of being placed on 4 DEG C, abandons precipitation to get alkali
Extract；
2）Isoelectric points of proteins precipitates：By step 1）Obtained alkali extract 4wt% sulphur acid for adjusting pH to 3.5, is placed on centrifuge
6000 r/min centrifuge 6 min and are precipitated to get thick protein；
3）It is prepared by protein powder：By step 2）It is super for 3 kD using molecular cut off after obtained crude protein precipitation is redissolved
Filter membrane carry out desalination and concentration, then concentrate through 55 DEG C of 24 h of heated-air drying to get protein powder；
4）The protein content for the algae-residue protein powder that this method is prepared is 84wt%.
Embodiment 2
One kind method for extracting proteins from microalgae algae-residue, includes the following steps：
1）Algae-residue protein alkalinity extraction：The Du Shi that 1000 g have carried algae oil fat is added in 100 L 10wt% sodium hydroxide solutions
In algae algae-residue, agitating and heating extracts 50 min under the conditions of 45 DEG C, and reaction is placed on 8000 r/min on centrifuge and centrifuges 5 min,
Precipitation is abandoned to get alkali extract；
2）Isoelectric points of proteins precipitates：By step 1）Obtained alkali extract 72wt% sulphur acid for adjusting pH to 4.0, uses 0.2 μm
Micro-filtrate membrane filtration precipitates to get thick protein；
3）It is prepared by protein powder：By step 2）The use of molecular cut off is 250 Da after obtained crude protein precipitation is redissolved
NF membrane carries out desalination and concentration, and concentrate is 60 DEG C in temperature, and vacuum degree is dried in vacuo 24 h under conditions of being -0.08 MPa,
Up to protein powder；
4）The protein content for the algae-residue protein powder that this method is prepared is 82wt%.
Embodiment 3
One kind method for extracting proteins from microalgae algae-residue, includes the following steps：
1）Algae-residue protein alkalinity extraction：1250 g are added in 100 L 20wt% sodium hydroxide solutions and have carried phycochrome grease
In chlamydomonas algae-residue, agitating and heating extracts 25 min under the conditions of 60 DEG C, and 0.2 μm of micro-filtrate membrane filtration is used after reaction, abandons precipitation, i.e.,
Obtain alkali extract；
2）Isoelectric points of proteins precipitates：By step 1）Obtained alkali extract 6 mol/L salt acid for adjusting pH to 4.5, are placed in centrifugation
10000 r/min centrifuge 3 min and are precipitated to get thick protein on machine；
3）It is prepared by protein powder：By step 2）It is super for 10 kD using molecular cut off after obtained crude protein precipitation is redissolved
Chimney filter carries out desalination and concentration, and freeze-dried 24 h of concentrate is to get protein powder；
4）The protein content for the algae-residue protein powder that this method is prepared is 86wt%.
Embodiment 4
One kind method for extracting proteins from microalgae algae-residue, includes the following steps：
1）Algae-residue protein alkalinity extraction：The spiral that 1500 g have carried algae oil fat is added in 100 L 15wt% sodium hydroxide solutions
In algae algae-residue, agitating and heating extracts 10 min under the conditions of 80 DEG C, and reaction is placed on 10000 r/min on centrifuge and centrifuges 4 min,
Precipitation is abandoned to get alkali extract；
2）Isoelectric points of proteins precipitates：By step 1）Obtained alkali extract 6 mol/L salt acid for adjusting pH to 5.5, are placed in 4 DEG C of ice
8 h are stood in case to precipitate to get thick protein；
3）It is prepared by protein powder：By step 2）It is super for 30 KD using molecular cut off after obtained crude protein precipitation is redissolved
Filter membrane carries out desalination and concentration, and concentrate is spray-dried, and dry condition is：2.0 m of intake3/ min, charging rate 1.2
L/h, 160 DEG C of inlet air temperature are to get protein powder；
4）The protein content for the algae-residue protein powder that this method is prepared is 90wt%.
The foregoing is merely the representative embodiments of the present invention, are not intended to restrict the invention, although with reference to aforementioned
Invention is explained in detail for embodiment, for those skilled in the art, still can be to aforementioned each reality
The technical solution recorded in example is applied to modify or equivalent replacement of some of the technical features.It is all the present invention
Within spirit and principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (9)
1. one kind method for extracting proteins from microalgae algae-residue, it is characterised in that：Include the following steps：
1）Algae-residue protein alkalinity extraction：Sodium hydroxide solution is added in algae-residue, heating extraction is stirred, is separated by solid-liquid separation
Afterwards to get alkali extract；
2）Isoelectric points of proteins precipitates：By step 1）Obtained alkali extract is adjusted with acid pH to acidity, to get thick after separation of solid and liquid
Protein precipitation；
3）It is prepared by protein powder：By step 2）Obtained crude protein precipitation redissolved successively, desalination and concentration, dry protein
Powder.
2. one kind method for extracting proteins from microalgae algae-residue according to claim 1, it is characterised in that：The step
1）In, algae-residue is to have extracted in phycochrome, algae oil fat, phycochrome grease and polysaccharides remaining part after any ingredient.
3. one kind method for extracting proteins from microalgae algae-residue according to claim 1, it is characterised in that：The step
1）In, the algae in algae-residue source is chlorella, Du Shi algaes, micro- quasi- ball algae, chlamydomonas, scenedesmus, Phaeodactylum tricornutum, purple ball algae, spiral
One kind in algae, chrysophyceae, hidden dinoflagellate and haematococcus pluvialis.
4. one kind method for extracting proteins from microalgae algae-residue according to claim 1, it is characterised in that：The step
1）In, naoh concentration 1wt%-30wt%, extraction conditions are：Solid-to-liquid ratio is 7.5-15:1 g/L, temperature be 30-80 DEG C,
Extraction time is 10-60 min.
5. one kind method for extracting proteins from microalgae algae-residue according to claim 1, it is characterised in that：The step
1）With step 2）In, solid-liquid separating method is one kind in standing, centrifuge and filtering.
6. one kind method for extracting proteins from microalgae algae-residue according to claim 1, it is characterised in that：The step
2）In, acid is sulfuric acid or hydrochloric acid, adjusts pH to 3.5-5.5.
7. one kind method for extracting proteins from microalgae algae-residue according to claim 1, it is characterised in that：The step
3）In, desalination and concentration method is ultrafiltration or nanofiltration.
8. one kind method for extracting proteins from microalgae algae-residue according to claim 1, it is characterised in that：The step
3）In, drying means is one kind in heated-air drying, vacuum drying, freeze-drying and spray drying.
9. a kind of algae-residue protein powder as made from claim 1-8 any one of them extracting methods, it is characterised in that：Algae-residue
The protein content of protein powder is 80wt% ~ 95wt%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112553191A (en) * | 2020-12-14 | 2021-03-26 | 恩施藻缘硒肽生物科技有限公司 | Method for efficiently extracting selenoprotein and application |
| WO2021229297A1 (en) * | 2020-05-13 | 2021-11-18 | Sophie's BioNutrients Pte. Ltd. | Methods of producing plant protein from food waste using microalgae |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001072974A3 (en) * | 2000-03-29 | 2002-01-31 | Ascorbex Ltd | L-galactose dehydrogenase from arabidopsis thaliana, and uses thereof |
| CN101429467A (en) * | 2008-12-24 | 2009-05-13 | 青岛生物能源与过程研究所 | Method for simultaneously extracting lipid and protein from microalgae |
| CN101575359A (en) * | 2008-05-05 | 2009-11-11 | 青岛海之骄生物科技有限公司 | Extraction method of water-soluble spirulina phycobiliprotein |
| CN105001971A (en) * | 2015-06-26 | 2015-10-28 | 新奥科技发展有限公司 | Two-step production method for microalgae bio-oil |
-
2018
- 2018-05-21 CN CN201810488168.XA patent/CN108409828A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001072974A3 (en) * | 2000-03-29 | 2002-01-31 | Ascorbex Ltd | L-galactose dehydrogenase from arabidopsis thaliana, and uses thereof |
| CN101575359A (en) * | 2008-05-05 | 2009-11-11 | 青岛海之骄生物科技有限公司 | Extraction method of water-soluble spirulina phycobiliprotein |
| CN101429467A (en) * | 2008-12-24 | 2009-05-13 | 青岛生物能源与过程研究所 | Method for simultaneously extracting lipid and protein from microalgae |
| CN105001971A (en) * | 2015-06-26 | 2015-10-28 | 新奥科技发展有限公司 | Two-step production method for microalgae bio-oil |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021229297A1 (en) * | 2020-05-13 | 2021-11-18 | Sophie's BioNutrients Pte. Ltd. | Methods of producing plant protein from food waste using microalgae |
| CN112553191A (en) * | 2020-12-14 | 2021-03-26 | 恩施藻缘硒肽生物科技有限公司 | Method for efficiently extracting selenoprotein and application |
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