CN106883313A - A kind of method that pectin is extracted from lemon peel - Google Patents
A kind of method that pectin is extracted from lemon peel Download PDFInfo
- Publication number
- CN106883313A CN106883313A CN201510935481.XA CN201510935481A CN106883313A CN 106883313 A CN106883313 A CN 106883313A CN 201510935481 A CN201510935481 A CN 201510935481A CN 106883313 A CN106883313 A CN 106883313A
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- China
- Prior art keywords
- pectin
- lemon peel
- ionic liquid
- treatment
- cellulose
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
Abstract
The invention discloses a kind of extracting method of pectin from lemon peel, comprise the following steps：（1）Lemon peel is processed using ionic liquid, the compositions such as pectin in lemon peel and cellulose are separated；（2）After ionic liquid treatment, water is added the pectin in lemon peel is dissolved in the composition Precipitations such as water, cellulose, hemicellulose and lignin；（3）Supernatant liquid is taken, cellulase treatment is added, cellulose impurities are removed；（4）Processed through cellulase and remove deimpurity liquid by after desolventing technology, addition ethanol makes pectin Precipitation, and with absolute ethanol washing, drying and grinding into powder obtains citrus pectin.Ionic liquid of the invention, microwave and cellulase Integration ofTechnology extract pectin in lemon peel, can only need ionic liquid to process 8-12min with high efficiency extraction pectin, and final pectin extraction rate can reach 25% or so, improve the pectin extraction efficiency of lemon peel.
Description
Technical field
The invention belongs to biological technical field, and in particular to be it is a kind of from lemon peel extract pectin method.
Background technology
Lemon peel constitutes about the 20%~30% of lemon quality, is a kind of reproducibility living resources that can be developed.Contain many bioactive ingredients in lemon peel,
Wherein pectin mass fraction is 25%~30%.
There are pectin the different physiological roles such as antibacterial, reducing blood lipid, radioresistance and anticancer to act on,
It has been widely used in industries such as food, chemical industry, medicine in recent years.If lemon peel is directly abandoned, not only greatly waste of resource, and surrounding enviroment are polluted.Therefore,
The pectin in lemon peel is efficiently extracted, added value of product is improved, environmental pollution is reduced significant.
At present, pectin extraction typically has boiling water extracting, acid system extraction, ion-exchange, microbial method and ammonium oxalate extraction method, and the method for most common of which is acidity extraction.Traditional acidity extraction has that strongly acidic solution consumption is more, power consumption is big, working strength is big, the low shortcoming of extraction efficiency, and it further develops and has been restricted.Microwave-assisted extraction technique is to develop green separation technique faster in recent years,
Have the advantages that easy, efficient, selectivity is strong, have much development potentiality.However, highly acid extractant meeting etching apparatus, can also cause the glycosidic bond in pectin to be broken.Therefore,
Selection utilization is efficient, the extractant of the feature of environmental protection is very necessary.In recent years, ionic liquid is widely used to the extract and separate of bioactivator as a kind of green solvent, but the extraction for pectin is rarely reported.
There is presently no the report that report prepares pectin using ionic liquid, microwave and cellulase integrated technology.
The content of the invention
It is an object of the invention to be directed to the low deficiency of pectin extraction efficiency in the prior art, there is provided a kind of method that utilization ionic liquid, microwave and cellulase Integration ofTechnology extract pectin from lemon peel.
Technical scheme：The extracting method of pectin from lemon peel of the present invention, comprises the following steps：
（1）Fresh Lemon skin is blended,
Its 3.5 times of water of quality, the min of microwave treatment 15 are added to rinse raw material to rinsing liquid in colourless with distilled water；
（2）With ionic liquid aqueous solution it is extractant and combines microwave treatment lemon peel, separates the compositions such as pectin in lemon peel and cellulose；
（3）After ionic liquid treatment, water is added the pectin in lemon peel is dissolved in the composition Precipitations such as water, cellulose, hemicellulose and lignin；
（4）Supernatant liquid is taken, cellulase treatment is added, the temperature for the treatment of is 40-50 DEG C, and process time is 5-20h, removes cellulose impurities；
（5）Processed through cellulase and remove deimpurity liquid, add ethanol to make pectin Precipitation, with absolute ethanol washing, drying and grinding into powder obtains citrus pectin.
Preferably, the ionic liquid is that concentration is 1.0 mol/L [BMM] Cl (chlorination 1- butyl -3- methylimidazoles).
It is further preferred that the temperature of the ionic liquid treatment lemon peel is 85-95 DEG C.
Still more preferably, the time of the ionic liquid treatment lemon peel is 8-12min.
It is an advantage of the invention that：
1st, the inventive method ionic liquid, microwave and enzyme treatment technique is integrated uses, extracting method environmental protection, ionic liquid and cellulase can be recycled and reused, it is to avoid excessive waste water, waste gas in production process, the need for meeting sustainable development.
2nd, the present invention is pectin in inorganic agent extraction lemon peel using ionic liquid, can only need ionic liquid to process 8-12min with high efficiency extraction pectin, and final pectin yield can reach 25% or so, improve the pectin extraction efficiency of lemon peel.
3rd, it be raw material that the present invention use reproducible lemon discarded object, abundant raw material, and regeneration cycle rapidly, contributes to solution there is lack of raw materials problem, while realizing the high level conversion of lemon discarded object.
Specific embodiment
With reference to embodiment, the present invention will be further described：
Embodiment 1
（1）Fresh Lemon skin is blended,
Its 3.5 times of water of quality, the min of microwave treatment 15 are added to rinse raw material to rinsing liquid in colourless with distilled water, obtain lemon peel slurry；
（2）100g lemon peel slurries are taken, 300ml 1.0 is added
Mol/L [BMM] Cl (chlorination 1- butyl -3- methylimidazoles), under the conditions of 90 DEG C and the W of microwave power 400, processes lemon peel 8-12min, and the precipitation of material such as cellulose are separated out to 1000-3000mL water is added in system；
（3）Supernatant liquid is taken, cellulase to final concentration 5FPU/mL is added, 12h is processed under conditions of being 45 DEG C in temperature, remove the cellulose impurities mixed in pectin, obtain liquid pectin；
（4）Liquid pectin removes most of water in 80-90 DEG C of vacuum distillation, adds absolute ethyl alcohol to make pectin Precipitation, is precipitated 2 times with absolute ethanol washing, it is vacuum dried under the conditions of 60 DEG C, pulverizer is crushed, and obtains mulberry pectin powder 5.03g, is 25.15% equivalent to dried lemon peel recovery rate.
Note：Pectin extraction rate/%=(quality of the pectin quality/dried lemon peel of extraction) × 100
Embodiment 2
（1）Fresh Lemon skin is blended,
Its 3.5 times of water of quality, the min of microwave treatment 15 are added to rinse raw material to rinsing liquid in colourless with distilled water, obtain lemon peel slurry；
（2）Take 100g lemon peel slurries, add 200ml 1.0 mol/L [BMM] Cl (chlorination 1- butyl -3- methylimidazoles), under the conditions of 85 DEG C and the W of microwave power 400, lemon peel 8-12min is processed, the precipitation of material such as cellulose are separated out to 1000-4000mL water is added in system；
（3）Supernatant liquid is taken, cellulase to final concentration 6FPU/mL is added, 4-12h is processed under conditions of being 50 DEG C in temperature, remove the cellulose impurities mixed in pectin, obtain liquid pectin；
（4) storng-acid cation exchange resin and weak-base anion-exchange resin pillar that liquid pectin passes through 60cm high；
（5）Liquid pectin is precipitated 2 times in 80-90 DEG C of vacuum distillation moisture removal with absolute ethanol washing, is vacuum dried under the conditions of 60 DEG C, and pulverizer is crushed, and obtains mulberry pectin powder 5.16g, is 25.8% equivalent to dried lemon peel recovery rate.
Note：Pectin extraction rate/%=(quality of the pectin quality/dried lemon peel of extraction) × 100
Although as described above, having been represented with reference to specific preferred embodiment and having described the present invention, it shall not be construed as to the limitation of itself of the invention.Under the premise of the spirit and scope of the present invention for not departing from appended claims definition, can various changes can be made in the form and details to it.
Claims (4)
1. it is a kind of from lemon peel extract pectin method, it is characterised in that comprise the following steps：
（1）Fresh Lemon skin is blended, its 3.5 times of water of quality are added,
The min of microwave treatment 15, raw material is rinsed to rinsing liquid in colourless with distilled water；
（2）With ionic liquid aqueous solution it is extractant and combines microwave treatment lemon peel, separates the compositions such as pectin in lemon peel and cellulose；
（3）After ionic liquid treatment, water is added the pectin in lemon peel is dissolved in the composition Precipitations such as water, cellulose, hemicellulose and lignin；
（4）Supernatant liquid is taken, cellulase treatment is added, the temperature for the treatment of is 40-50 DEG C, and process time is 5-20h, removes cellulose impurities；
（5）Processed through cellulase and remove deimpurity liquid, add ethanol to make pectin Precipitation, with absolute ethanol washing, drying and grinding into powder obtains citrus pectin.
2. the extracting method of the pectin from lemon peel according to claim 1, it is characterised in that：The ionic liquid is that concentration is 1.0 mol/L
[BMM] Cl (chlorination 1- butyl -3- methylimidazoles).
3. the extracting method of the pectin from lemon peel according to claim 1, it is characterised in that：The temperature of the ionic liquid treatment lemon peel is 85-95 DEG C.
4. the extracting method of the pectin from lemon peel according to claim 1, it is characterised in that：The time of the ionic liquid treatment lemon peel is 8-12min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510935481.XA CN106883313A (en) | 2015-12-15 | 2015-12-15 | A kind of method that pectin is extracted from lemon peel |
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| CN201510935481.XA CN106883313A (en) | 2015-12-15 | 2015-12-15 | A kind of method that pectin is extracted from lemon peel |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107443519A (en) * | 2017-07-31 | 2017-12-08 | 惠州市格农科技有限公司 | The improved properties processing method of sheet material |
| CN107486903A (en) * | 2017-07-31 | 2017-12-19 | 惠州市格农科技有限公司 | The surface treatment method of longan sheet material |
| CN108402252A (en) * | 2018-05-17 | 2018-08-17 | 大连民族大学 | A kind of Schisandra chinensis multiple elements design tea beverage |
| CN108822305A (en) * | 2018-06-27 | 2018-11-16 | 西北农林科技大学 | A method of it is pre-processed based on glyoxaline ion liquid and extracts gutta-percha in eucommia bark |
| CN110041446A (en) * | 2019-03-20 | 2019-07-23 | 肖兵 | A kind of biomass comprehensive method for refining |
-
2015
- 2015-12-15 CN CN201510935481.XA patent/CN106883313A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107443519A (en) * | 2017-07-31 | 2017-12-08 | 惠州市格农科技有限公司 | The improved properties processing method of sheet material |
| CN107486903A (en) * | 2017-07-31 | 2017-12-19 | 惠州市格农科技有限公司 | The surface treatment method of longan sheet material |
| CN108402252A (en) * | 2018-05-17 | 2018-08-17 | 大连民族大学 | A kind of Schisandra chinensis multiple elements design tea beverage |
| CN108822305A (en) * | 2018-06-27 | 2018-11-16 | 西北农林科技大学 | A method of it is pre-processed based on glyoxaline ion liquid and extracts gutta-percha in eucommia bark |
| CN108822305B (en) * | 2018-06-27 | 2020-11-27 | 西北农林科技大学 | Method for extracting gutta-percha from eucommia bark based on imidazole ionic liquid pretreatment |
| CN110041446A (en) * | 2019-03-20 | 2019-07-23 | 肖兵 | A kind of biomass comprehensive method for refining |
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Application publication date: 20170623 |