CN106215043A - A kind of roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar and preparation method thereof - Google Patents
A kind of roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the preparation field of health product, be specifically related to a kind of roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar and preparation method thereof.This roxburgh anoectochilus terminal bud buccal tablet includes following raw material by weight: Herba Anoectochili roxburghii 25 35 parts, Radix Salviae Miltiorrhizae 35 parts, the Rhizoma Anemarrhenae 6 10 parts, 68 parts of Fructus Hordei Germinatus, limacia sagittata 13 parts, Herb Gynostemmae Pentaphylli 12 15 parts, Fructus Lycii 46 parts, Ganoderma 58 parts and Herba Dendrobii 23 parts.The present invention is directed to the feature of each raw material, extract preparation accordingly, make effective active constituents extraction rate high, active substance after extracting makes roxburgh anoectochilus terminal bud buccal tablet, has an obvious hypoglycemic activity, and can strengthening the body resistance, improve body immunity, prevent and treat other complication.
Description
Technical field
The invention belongs to the preparation field of health product, be specifically related to a kind of roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar and
Preparation method.
Background technology
When (sugar-free and any sugary food intake in 8 hours) blood glucose is higher than normal range, referred to as hyperglycemia on an empty stomach.Fasting glucose
Normal value 4.0-6.1mmol/L, when two hours blood glucoses are higher than 7.8mmol/L normal range after the meal, it is also possible to be referred to as hyperglycemia.Just
In the case of Chang, by hormonal regulation and this two big regulation system of neuroregulation, human body can guarantee that the source of blood glucose keeps with outlet
Balance, makes blood glucose maintain certain level.But at inherited genetic factors (such as Diabetes family history) with environmental factors (as irrational
Meals, obesity etc.) common effect under, two big regulatory functions get muddled, and arise that the rising of blood sugar level.
Human body is damaged by short time, disposable hyperglycemia without serious.Such as under stress state or excited, highly
Time nervous, may occur in which of short duration hyperglycemia；Once take food substantial amounts of saccharide, it is possible to of short duration hyperglycemia occurs；Subsequently, blood sugar level
Gradually recover normal.But long-term hyperglycemia can make each histoorgan generation pathological changes of whole body, causes acute and chronic complication
Occur.As pancreatic function exhaustion, lose water and rock-soil coupling, malnutrition, resistance decline, impaired renal function, neuropathy,
Retinopathy etc..
Antidiabetic drug the most on the market is generally divided into Chinese medicine antidiabetic drug and Western medicine antidiabetic drug.The side effect of Western medicine antidiabetic drug is
Being easily caused hypoglycemia, such as alpha-glucosidase inhibitor, biguanides and euglycemic agent are used alone and typically will not cause low blood
Sugar, but when being used in conjunction with other drug still it may happen that.Patient likely there will be strong feel on an empty stomach, be in a cold sweat, general weakness, the heart
Throb with fear, trick is shaken, eyes grow dim, headache, the phenomenon such as stupefied, can go into a coma time serious.
And when using Chinese medicine antidiabetic drug to treat, it is not only in that reduction blood glucose, it is often more important that focus on preventing and treating concurrent
Disease, safety is higher, plays and improves the quality of living and prolong long-life effect.
Summary of the invention
Present invention aims to the deficiency of existing hypoglycemic drug, it is provided that a kind of gold thread with effect of lowering blood sugar
Lotus buccal tablet and preparation method thereof.This roxburgh anoectochilus terminal bud buccal tablet biological activity is high, mouthfeel is good, has heat-clearing and toxic substances removing, expelling wind and removing dampness, protects the liver and protect
The effects such as liver, invigorating the lung and the kidney, benefiting vital QI for tranquillizing and reduction hyperglycemia.
For realizing the purpose of the present invention, adopt the following technical scheme that
A kind of roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar, by weight, including following raw material: Herba Anoectochili roxburghii 25-35 part, Radix Salviae Miltiorrhizae
3-5 part, Rhizoma Anemarrhenae 6-10 part, Fructus Hordei Germinatus 6-8 part, limacia sagittata 1-3 part, Herb Gynostemmae Pentaphylli 12-15 part, Fructus Lycii 4-6 part, Ganoderma 5-8 part and stone
Dry measure used in former times 2-3 part.
Preferably, the described roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar, by weight, including following raw material: gold thread
30 parts of lotus, Radix Salviae Miltiorrhizae 4 parts, the Rhizoma Anemarrhenae 8 parts, 7 parts of Fructus Hordei Germinatus, limacia sagittata 2 parts, Herb Gynostemmae Pentaphylli 14 parts, Fructus Lycii 5 parts, Ganoderma 6 parts and Herba Dendrobii 2 parts.
A kind of method preparing the roxburgh anoectochilus terminal bud buccal tablet as above with effect of lowering blood sugar, comprises the following steps:
1) adding 5-8 times of weight portion volume fraction of Herba Anoectochili roxburghii herb is the ethanol solution of 80-90%, and room temperature adverse current soaks 4-6h；So
After Herba Anoectochili roxburghii is soaked system heating and refluxing extraction 2 times, each extraction time is 15-20min, merges 2 extracting solution, concentrated
After extractum 1；Medicinal residues after alcohol extraction again through 3-4 times of weight portion water in 60-70 DEG C extraction 6-8h after extracting solution 1；
2) after Radix Salviae Miltiorrhizae, Herb Gynostemmae Pentaphylli and Herba Dendrobii being mixed, add the water of 6-10 times of weight portion, extract 6-8h at 80-90 DEG C, filter
Extracting solution for the first time；In filtering residue, continuously add the water of 3-5 times of weight portion, extract 4-5h at 65-75 DEG C, filter to carry for the second time
Take liquid；In filtering residue, continuously add the water of 1.5-2 times of weight portion, extract 1-2h at 40-60 DEG C, filter to obtain third time extracting solution；
Merge three extracting solution and obtain extracting solution 2；
3), after the Rhizoma Anemarrhenae and limacia sagittata being mixed, adding 6-8 times of weight portion volume fraction is the ethanol solution of 75%, 75-85 DEG C of backflow
Extracting 3 times, each extraction time is 1-2h, merges 3 extracting solution, concentrated after extractum 2；
4) after Fructus Lycii, Ganoderma and Fructus Hordei Germinatus being mixed, add the water of 3-5 times of weight portion, extract 3-4h at 70-75 DEG C, filter to carry
Take liquid 3；Adding 6-8 times of weight portion volume fraction in filtering residue is the ethanol solution of 75%, extracts 4-6h, extracting solution at 75-85 DEG C
Extractum 3 is obtained after concentrated；
5) after extracting solution 1, extracting solution 2 and extracting solution 3 being merged ,-30 ~-10 DEG C, vacuum be 10-20Pa under conditions of cold
After the dry 10-12h of lyophilizing, prepare lyophilized powder；
6) by lyophilized powder, extractum 1, extractum 2 and extractum 3 mix homogeneously, tabletting, it is dried, sterilizing, prepares roxburgh anoectochilus terminal bud buccal tablet.
Preferably, described preparation has the method for the roxburgh anoectochilus terminal bud buccal tablet of effect of lowering blood sugar, comprises the following steps:
1) adding 6 times of weight portion volume fractions of Herba Anoectochili roxburghii herb is the ethanol solution of 85%, and room temperature adverse current soaks 5h；Then by gold
Anoectochilus roxburghii soaks system heating and refluxing extraction 2 times, and each extraction time is 20min, merges 2 extracting solution, concentrated after obtain extractum
1；Medicinal residues after alcohol extraction again through 4 times of weight portions water in 67 DEG C extraction 7h after extracting solution 1；
2) after Radix Salviae Miltiorrhizae, Herb Gynostemmae Pentaphylli and Herba Dendrobii being mixed, add the water of 8 times of weight portions, extract 7h at 86 DEG C, filter to carry for the first time
Take liquid；In filtering residue, continuously add the water of 4 times of weight portions, extract 4h at 72 DEG C, filter to obtain second time extracting solution；Relay at filtering residue
The continuous water adding 1.5 times of weight portions, extracts 1.5h at 50 DEG C, filters to obtain third time extracting solution；Merge three extracting solution must extract
Liquid 2；
3), after the Rhizoma Anemarrhenae and limacia sagittata being mixed, adding 7 times of weight portion volume fractions is the ethanol solution of 75%, 80 DEG C of reflux, extract, 3
Secondary, each extraction time is 1.5h, merges 3 extracting solution, concentrated after extractum 2；
4) after Fructus Lycii, Ganoderma and Fructus Hordei Germinatus being mixed, add the water of 4 times of weight portions, extract 3.5h at 72 DEG C, filter to obtain extracting solution 3；
Adding 7 times of weight portion volume fractions in filtering residue is the ethanol solution of 75%, extracts 5h at 80 DEG C, must soak after extracting solution is concentrated
Cream 3；
5) after extracting solution 1, extracting solution 2 and extracting solution 3 being merged ,-20 DEG C, vacuum be 15Pa under conditions of lyophilization
After 12h, prepare lyophilized powder；
6) by lyophilized powder, extractum 1, extractum 2 and extractum 3 mix homogeneously, tabletting, it is dried, sterilizing, prepares roxburgh anoectochilus terminal bud buccal tablet.
The beneficial effects of the present invention is:
1) present invention is with Herba Anoectochili roxburghii as primary raw material, and Herba Anoectochili roxburghii enters lung, liver, kidney, bladder warp, can Yin Yang balancing, strengthening the body resistance, tune
And QI and blood, improve body immunity comprehensively；Fructus Hordei Germinatus has aid digestion, fall hyperlipidemia, hypoglycemic activity, can improve unreasonable meals
The hyperglycemia caused；Limacia sagittata has obvious heat-clearing and toxic substances removing, hypoglycemic activity；Herb Gynostemmae Pentaphylli have regulating lipid and reducing weight, invigorating the stomach and promoting digestion,
Benefiting vital QI for tranquillizing, antipyretic and antidote functions；Ganoderma has the effects such as protecting liver and detoxication, strengthening the body resistance, reduction blood fat and blood glucose；Herba Dendrobii has
Reinforcing stomach reg fluid, nourishing YIN and clearing away heat, add essence and mend marrow, qi-restoratives and hold up the effect of win, hyperglycemia is caused dim-sighted, headache is the most largely effective；
Radix Salviae Miltiorrhizae has and improves microcirculation, blood sugar lowering and the effect such as antibacterial；The Rhizoma Anemarrhenae can let out clearly the fire of lung stomach, again can the moon of nourishing the lung and kidney,
There is obvious hypoglycemic activity；Fructus Lycii can be remarkably reinforced the activity of superoxide dismutase in damaged islet cells, improves pancreas
The oxidation resistance of island cell is useful to blood sugar lowering；Nine medical materials, according to tcm theory, are carried out compatibility, from multilamellar by the present invention
Secondary, multipath, Mutiple Targets reach heat-clearing and toxic substances removing, expelling wind and removing dampness, hepatoprotective, invigorating the lung and the kidney, benefiting vital QI for tranquillizing and reduction hyperglycemia
Effect；Although the most single medicine possesses the partial efficacy such as blood sugar lowering or heat-clearing and toxic substances removing, but is used alone and is unable to reach
The comprehensive effect of compound buccal lozenge of the present invention, roxburgh anoectochilus terminal bud buccal tablet of the present invention can be avoided as much as the generation of other complication, and
Reduce the hyperglycemia infringement to each organ；
2) Herba Anoectochili roxburghii herb adverse current in ethanol solution is soaked 4-6h by the present invention so that the effective active composition of Herba Anoectochili roxburghii is in room
It is fully dissolved out under temperature, the most again through the heating and refluxing extraction of short time, effective ingredient can be extracted expeditiously, and fully
Protect its activity, it is to avoid long-time high-temperature heating extracts the infringement to active component；
3) Radix Salviae Miltiorrhizae, Herb Gynostemmae Pentaphylli and Herba Dendrobii are used repeatedly by the present invention, different temperatures interval carries out step by step arithmetic, farthest carry
Getting various active component, and each active component there occurs certain conversion reaction in different temperature ranges, effect becomes apparent from；
4) present invention prepares powder after lyophilised for the extracting solution of each raw material, adds the extractum after concentration, has one after mixing
Fixed viscosity, is easier to molding during tabletting.
Detailed description of the invention
For the openest rather than restriction present invention, below in conjunction with example, the present invention is described in further detail.
Embodiment 1
A kind of roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar, by weight, including following raw material: Herba Anoectochili roxburghii 25 parts, Radix Salviae Miltiorrhizae 5
Part, the Rhizoma Anemarrhenae 6 parts, 8 parts of Fructus Hordei Germinatus, limacia sagittata 1 part, Herb Gynostemmae Pentaphylli 15 parts, Fructus Lycii 4 parts, Ganoderma 8 parts and Herba Dendrobii 2 parts.
A kind of method preparing the roxburgh anoectochilus terminal bud buccal tablet as above with effect of lowering blood sugar, comprises the following steps:
1) adding 5 times of weight portion volume fractions of Herba Anoectochili roxburghii herb is the ethanol solution of 90%, and room temperature adverse current soaks 4h；Then by gold
Anoectochilus roxburghii soaks system heating and refluxing extraction 2 times, and each extraction time is 20min, merges 2 extracting solution, concentrated after obtain extractum
1；Medicinal residues after alcohol extraction again through 4 times of weight portions water in 70 DEG C extraction 6h after extracting solution 1；
2) after Radix Salviae Miltiorrhizae, Herb Gynostemmae Pentaphylli and Herba Dendrobii being mixed, add the water of 6 times of weight portions, extract 8h at 80 DEG C, filter to carry for the first time
Take liquid；In filtering residue, continuously add the water of 3 times of weight portions, extract 4h at 75 DEG C, filter to obtain second time extracting solution；Relay at filtering residue
The continuous water adding 1.5 times of weight portions, extracts 1h at 40 DEG C, filters to obtain third time extracting solution；Merge three extracting solution and obtain extracting solution
2；
3), after the Rhizoma Anemarrhenae and limacia sagittata being mixed, adding 6 times of weight portion volume fractions is the ethanol solution of 75%, 75 DEG C of reflux, extract, 3
Secondary, each extraction time is 1h, merges 3 extracting solution, concentrated after extractum 2；
4) after Fructus Lycii, Ganoderma and Fructus Hordei Germinatus being mixed, add the water of 3 times of weight portions, extract 4h at 70 DEG C, filter to obtain extracting solution 3；?
Adding 6 times of weight portion volume fractions in filtering residue is the ethanol solution of 75%, extracts 6h at 75 DEG C, obtains extractum after extracting solution is concentrated
3；
5) after extracting solution 1, extracting solution 2 and extracting solution 3 being merged ,-30 DEG C, vacuum be 20Pa under conditions of lyophilization
After 10h, prepare lyophilized powder；
6) by lyophilized powder, extractum 1, extractum 2 and extractum 3 mix homogeneously, tabletting, it is dried, sterilizing, prepares roxburgh anoectochilus terminal bud buccal tablet.
Embodiment 2
A kind of roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar, by weight, including following raw material: Herba Anoectochili roxburghii 30 parts, Radix Salviae Miltiorrhizae 4
Part, the Rhizoma Anemarrhenae 8 parts, 7 parts of Fructus Hordei Germinatus, limacia sagittata 2 parts, Herb Gynostemmae Pentaphylli 14 parts, Fructus Lycii 5 parts, Ganoderma 6 parts and Herba Dendrobii 2 parts.
Preparation has the method for the roxburgh anoectochilus terminal bud buccal tablet of effect of lowering blood sugar, comprises the following steps:
1) adding 6 times of weight portion volume fractions of Herba Anoectochili roxburghii herb is the ethanol solution of 85%, and room temperature adverse current soaks 5h；Then by gold
Anoectochilus roxburghii soaks system heating and refluxing extraction 2 times, and each extraction time is 20min, merges 2 extracting solution, concentrated after obtain extractum
1；Medicinal residues after alcohol extraction again through 4 times of weight portions water in 67 DEG C extraction 7h after extracting solution 1；
2) after Radix Salviae Miltiorrhizae, Herb Gynostemmae Pentaphylli and Herba Dendrobii being mixed, add the water of 8 times of weight portions, extract 7h at 86 DEG C, filter to carry for the first time
Take liquid；In filtering residue, continuously add the water of 4 times of weight portions, extract 4h at 72 DEG C, filter to obtain second time extracting solution；Relay at filtering residue
The continuous water adding 1.5 times of weight portions, extracts 1.5h at 50 DEG C, filters to obtain third time extracting solution；Merge three extracting solution must extract
Liquid 2；
3), after the Rhizoma Anemarrhenae and limacia sagittata being mixed, adding 7 times of weight portion volume fractions is the ethanol solution of 75%, 80 DEG C of reflux, extract, 3
Secondary, each extraction time is 1.5h, merges 3 extracting solution, concentrated after extractum 2；
4) after Fructus Lycii, Ganoderma and Fructus Hordei Germinatus being mixed, add the water of 4 times of weight portions, extract 3.5h at 72 DEG C, filter to obtain extracting solution 3；
Adding 7 times of weight portion volume fractions in filtering residue is the ethanol solution of 75%, extracts 5h at 80 DEG C, must soak after extracting solution is concentrated
Cream 3；
5) after extracting solution 1, extracting solution 2 and extracting solution 3 being merged ,-20 DEG C, vacuum be 15Pa under conditions of lyophilization
After 12h, prepare lyophilized powder；
6) by lyophilized powder, extractum 1, extractum 2 and extractum 3 mix homogeneously, tabletting, it is dried, sterilizing, prepares roxburgh anoectochilus terminal bud buccal tablet.
Embodiment 3
A kind of roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar, by weight, including following raw material: Herba Anoectochili roxburghii 35 parts, Radix Salviae Miltiorrhizae 3
Part, the Rhizoma Anemarrhenae 10 parts, 6 parts of Fructus Hordei Germinatus, limacia sagittata 3 parts, Herb Gynostemmae Pentaphylli 12 parts, Fructus Lycii 6 parts, Ganoderma 5 parts and Herba Dendrobii 3 parts.
A kind of method preparing the roxburgh anoectochilus terminal bud buccal tablet as above with effect of lowering blood sugar, comprises the following steps:
1) adding 8 times of weight portion volume fractions of Herba Anoectochili roxburghii herb is the ethanol solution of 80%, and room temperature adverse current soaks 6h；Then by gold
Anoectochilus roxburghii soaks system heating and refluxing extraction 2 times, and each extraction time is 20min, merges 2 extracting solution, concentrated after obtain extractum
1；Medicinal residues after alcohol extraction again through 4 times of weight portions water in 70 DEG C extraction 8h after extracting solution 1；
2) after Radix Salviae Miltiorrhizae, Herb Gynostemmae Pentaphylli and Herba Dendrobii being mixed, add the water of 10 times of weight portions, extract 8h at 80 DEG C, filter for the first time
Extracting solution；In filtering residue, continuously add the water of 5 times of weight portions, extract 5h at 75 DEG C, filter to obtain second time extracting solution；In filtering residue
Continuously add the water of 2 times of weight portions, extract 2h at 60 DEG C, filter to obtain third time extracting solution；Merge three extracting solution and obtain extracting solution
2；
3), after the Rhizoma Anemarrhenae and limacia sagittata being mixed, adding 8 times of weight portion volume fractions is the ethanol solution of 75%, 85 DEG C of reflux, extract,
3 times, each extraction time is 2h, merges 3 extracting solution, concentrated after extractum 2；
4) after Fructus Lycii, Ganoderma and Fructus Hordei Germinatus being mixed, add the water of 5 times of weight portions, extract 4h at 70 DEG C, filter to obtain extracting solution 3；?
Adding 8 times of weight portion volume fractions in filtering residue is the ethanol solution of 75%, extracts 6h at 75 DEG C, obtains extractum after extracting solution is concentrated
3；
5) after extracting solution 1, extracting solution 2 and extracting solution 3 being merged ,-10 DEG C, vacuum be 20Pa under conditions of lyophilization
After 12h, prepare lyophilized powder；
6) by lyophilized powder, extractum 1, extractum 2 and extractum 3 mix homogeneously, tabletting, it is dried, sterilizing, prepares roxburgh anoectochilus terminal bud buccal tablet.
The Ergonomy research of roxburgh anoectochilus terminal bud buccal tablet of the present invention
1) fasting glucose experiment is reduced: select hyperglycemia model animal to be grouped by the fasting blood sugar level of 3 ~ 5 hours, select 1 at random
Model control group and 3 dosage groups (between group, difference is not more than 1.1mmol/L).Experimental group gives variable concentrations given the test agent, model
Matched group gives solvent, continuous 30 days, surveys fasting blood sugar (before fasting is with experiment), relatively each treated animal blood glucose value and blood glucose
Decline percentage rate.
2) carbohydrate tolerance experiment: hyperglycemia model animal fasting 3 ~ 5 hours, experimental group gives variable concentrations given the test agent, mould
Type matched group gives same volume solvent, oral administration of glucose 2.0g/kg or medical starch 3 ~ 5g/kg after 15 ~ 20 minutes, surveys
Determine the blood glucose value to after glucose 0,0.5,2 hours or to the blood glucose value of 0,1,2 hours, observing and nursing pair after medical starch
According to group with given the test agent group to the change of time point Area under the curve of blood glucose each after glucose or medical starch.
Result judges: an index positive in fasting glucose and carbohydrate tolerance binomial index, and to intact animal's fasting glucose
Without impact, i.e. can determine that this given the test agent auxiliary hyperglycemic function results of animal is positive.
Pharmacodynamic experiment
1.1.1 medicine
Roxburgh anoectochilus terminal bud buccal tablet of the present invention (prepared by embodiment 2, is prepared as the reagent containing crude drug 0.2g/1ml)；Metformin hydrochloride
(Shanghai Fahrenheit pharmacy).
1.1.2 animal
Kunming mouse 96, male and female half and half, body weight (22.5 ± 2.5) g, carried by University Of Agriculture and Forestry In Fujian's animal experimental center
Supply.
1.1.3 reagent and instrument
Streptozotocin (STZ) (SERVER company), purchased from magnificent company.Electronic balance AB204-WO type (Switzerland Mei Te
Le-torr company limited)；Hitachi 7150 type automatic clinical chemistry analyzer (Japanese).
1.2 method
1.2.1 modeling is with packet diabetes: animal is bought back, after adaptability feeds 1 week, is randomly divided into normal non-diabetic group 8
With experimental group 40, experimental group is fasting 12 hours before modeling, and modeling uses the injection of STZ 55mg/kg disposable celiac, is administered
Within latter 24 hours, surveying blood glucose, glucose in urine, for three days on end, glucose in urine is qualitative +++ more than, blood glucose value is more than 16.5mmol/ L, and continues constant
Person, is defined as diabetes and is formed.
Modeling success mice is randomly divided into 5 groups, model control group, metformin group, the present invention high, medium and low concentration group.
Hyperlipemia: animal is bought back, after adaptability feeds 1 week, is randomly divided into normal non-hyperlipemia group 8 and experimental group
40, experimental group is fasting 12 hours before modeling, modeling give more than 1 week containing 1% cholesterol, 20% Adeps Sus domestica, 2% sodium cholate, 1% the third
The high lipoprotein emulsion of base sulfur pyrimidine, every day 1 time, continuous 7 days.Modeling success mice is randomly divided into 5 groups, model control group, diformazan
Biguanide group, the present invention high, medium and low concentration group.
1.2.2 be administered and assay method
Normal group and model control group (the isopyknic normal saline of ig)；Metformin hydrochloride group (ig, 0.20g/kg)；This
Invention roxburgh anoectochilus terminal bud buccal tablet group (with water dissolution) (ig every day buccal tablet of the present invention lysate 2.5g/kg, 3.5g/ kg, 4.5g/ respectively
Kg).Each group gastric infusion respectively, 1 time/d, continuous 30 days.Last is administered front 12h fasting, 2h after administration, plucks eye and takes blood, with complete
Automatic biochemistry analyzer measures blood glucose and blood fat.
1.2.3 statistical procedures
All data represent with "-χ ± S ", and data acquisition SPSS11.5 statistical software analyzes and processes, if comparing variance between many groups
Homogeneous one way analysis of variance, if heterogeneity of variance rank test, front and back compares in group with pairing t inspection.
2 results
2.1 blood sugar lowering experiments
From table 1, the present invention high, medium and low concentration group all can significantly reduce the blood glucose of hyperglycemia mouse model.
The impact (-χ ± S) on mouse blood sugar of table 1 roxburgh anoectochilus terminal bud buccal tablet of the present invention
2.2 blood fat reducing experiments
From table 2, the high, medium and low concentration of the present invention all can substantially reduce TC, TG content of hyperlipemia mice model.
The impact (χ ± S) on lipid of mice of table 2 roxburgh anoectochilus terminal bud buccal tablet of the present invention
The foregoing is only presently preferred embodiments of the present invention, all equalizations done according to scope of the present invention patent change and repair
Decorations, all should belong to the covering scope of the present invention.
Claims (4)
1. a roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar, it is characterised in that: by weight, including following raw material: gold thread
Lotus 25-35 part, Radix Salviae Miltiorrhizae 3-5 part, Rhizoma Anemarrhenae 6-10 part, Fructus Hordei Germinatus 6-8 part, limacia sagittata 1-3 part, Herb Gynostemmae Pentaphylli 12-15 part, Fructus Lycii 4-6 part,
Ganoderma 5-8 part and Herba Dendrobii 2-3 part.
The roxburgh anoectochilus terminal bud buccal tablet with effect of lowering blood sugar the most according to claim 1, it is characterised in that: by weight, bag
Include following raw material: Herba Anoectochili roxburghii 30 parts, Radix Salviae Miltiorrhizae 4 parts, the Rhizoma Anemarrhenae 8 parts, 7 parts of Fructus Hordei Germinatus, limacia sagittata 2 parts, Herb Gynostemmae Pentaphylli 14 parts, Fructus Lycii 5 parts, spirit
Sesame 6 parts and Herba Dendrobii 2 parts.
3. the method preparing the roxburgh anoectochilus terminal bud buccal tablet as claimed in claim 1 or 2 with effect of lowering blood sugar, its feature exists
In: comprise the following steps:
1) adding 5-8 times of weight portion volume fraction of Herba Anoectochili roxburghii herb is the ethanol solution of 80-90%, and room temperature adverse current soaks 4-6h；So
After Herba Anoectochili roxburghii is soaked system heating and refluxing extraction 2 times, each extraction time is 15-20min, merges 2 extracting solution, concentrated
After extractum 1；Medicinal residues after alcohol extraction again through 3-4 times of weight portion water in 60-70 DEG C extraction 6-8h after extracting solution 1；
2) after Radix Salviae Miltiorrhizae, Herb Gynostemmae Pentaphylli and Herba Dendrobii being mixed, add the water of 6-10 times of weight portion, extract 6-8h at 80-90 DEG C, filter
Extracting solution for the first time；In filtering residue, continuously add the water of 3-5 times of weight portion, extract 4-5h at 65-75 DEG C, filter to carry for the second time
Take liquid；In filtering residue, continuously add the water of 1.5-2 times of weight portion, extract 1-2h at 40-60 DEG C, filter to obtain third time extracting solution；
Merge three extracting solution and obtain extracting solution 2；
3), after the Rhizoma Anemarrhenae and limacia sagittata being mixed, adding 6-8 times of weight portion volume fraction is the ethanol solution of 75%, 75-85 DEG C of backflow
Extracting 3 times, each extraction time is 1-2h, merges 3 extracting solution, concentrated after extractum 2；
4) after Fructus Lycii, Ganoderma and Fructus Hordei Germinatus being mixed, add the water of 3-5 times of weight portion, extract 3-4h at 70-75 DEG C, filter to carry
Take liquid 3；Adding 6-8 times of weight portion volume fraction in filtering residue is the ethanol solution of 75%, extracts 4-6h, extracting solution at 75-85 DEG C
Extractum 3 is obtained after concentrated；
5) after extracting solution 1, extracting solution 2 and extracting solution 3 being merged ,-30 ~-10 DEG C, vacuum be 10-20Pa under conditions of cold
After the dry 10-12h of lyophilizing, prepare lyophilized powder；
6) by lyophilized powder, extractum 1, extractum 2 and extractum 3 mix homogeneously, tabletting, it is dried, sterilizing, prepares roxburgh anoectochilus terminal bud buccal tablet.
Preparation the most according to claim 3 has the method for the roxburgh anoectochilus terminal bud buccal tablet of effect of lowering blood sugar, it is characterised in that: include
Following steps:
1) adding 6 times of weight portion volume fractions of Herba Anoectochili roxburghii herb is the ethanol solution of 85%, and room temperature adverse current soaks 5h；Then by gold
Anoectochilus roxburghii soaks system heating and refluxing extraction 2 times, and each extraction time is 20min, merges 2 extracting solution, concentrated after obtain extractum
1；Medicinal residues after alcohol extraction again through 4 times of weight portions water in 67 DEG C extraction 7h after extracting solution 1；
2) after Radix Salviae Miltiorrhizae, Herb Gynostemmae Pentaphylli and Herba Dendrobii being mixed, add the water of 8 times of weight portions, extract 7h at 86 DEG C, filter to carry for the first time
Take liquid；In filtering residue, continuously add the water of 4 times of weight portions, extract 4h at 72 DEG C, filter to obtain second time extracting solution；Relay at filtering residue
The continuous water adding 1.5 times of weight portions, extracts 1.5h at 50 DEG C, filters to obtain third time extracting solution；Merge three extracting solution must extract
Liquid 2；
3), after the Rhizoma Anemarrhenae and limacia sagittata being mixed, adding 7 times of weight portion volume fractions is the ethanol solution of 75%, 80 DEG C of reflux, extract, 3
Secondary, each extraction time is 1.5h, merges 3 extracting solution, concentrated after extractum 2；
4) after Fructus Lycii, Ganoderma and Fructus Hordei Germinatus being mixed, add the water of 4 times of weight portions, extract 3.5h at 72 DEG C, filter to obtain extracting solution 3；
Adding 7 times of weight portion volume fractions in filtering residue is the ethanol solution of 75%, extracts 5h at 80 DEG C, must soak after extracting solution is concentrated
Cream 3；
5) after extracting solution 1, extracting solution 2 and extracting solution 3 being merged ,-20 DEG C, vacuum be 15Pa under conditions of lyophilization
After 12h, prepare lyophilized powder；
6) by lyophilized powder, extractum 1, extractum 2 and extractum 3 mix homogeneously, tabletting, it is dried, sterilizing, prepares roxburgh anoectochilus terminal bud buccal tablet.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106822647A (en) * | 2017-03-14 | 2017-06-13 | 华中农业大学 | A kind of hypoglycemic compound preparation of lipid-loweringing and preparation method thereof |
| CN107822134A (en) * | 2017-12-25 | 2018-03-23 | 云南保山嘉宏农业开发（集团）有限公司 | Roxburgh anoectochilus terminal bud buccal tablet and manufacture craft |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100828A (en) * | 2011-01-25 | 2011-06-22 | 王启凌 | Medicament for treating diabetes mellitus |
| CN102499968A (en) * | 2011-12-30 | 2012-06-20 | 杨洪英 | Drug for treating diabetes and preparation method thereof |
| CN102697748A (en) * | 2012-06-21 | 2012-10-03 | 厦门加晟生物科技有限公司 | Roxburgh anoectochilus terminal bud buccal tablet and preparation method thereof |
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2016
- 2016-09-06 CN CN201610803146.9A patent/CN106215043A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100828A (en) * | 2011-01-25 | 2011-06-22 | 王启凌 | Medicament for treating diabetes mellitus |
| CN102499968A (en) * | 2011-12-30 | 2012-06-20 | 杨洪英 | Drug for treating diabetes and preparation method thereof |
| CN102697748A (en) * | 2012-06-21 | 2012-10-03 | 厦门加晟生物科技有限公司 | Roxburgh anoectochilus terminal bud buccal tablet and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106822647A (en) * | 2017-03-14 | 2017-06-13 | 华中农业大学 | A kind of hypoglycemic compound preparation of lipid-loweringing and preparation method thereof |
| CN107822134A (en) * | 2017-12-25 | 2018-03-23 | 云南保山嘉宏农业开发（集团）有限公司 | Roxburgh anoectochilus terminal bud buccal tablet and manufacture craft |
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