CN106214958A - A kind of health beverage of slow down aging and preparation method thereof - Google Patents
A kind of health beverage of slow down aging and preparation method thereof Download PDFInfo
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- CN106214958A CN106214958A CN201610724451.9A CN201610724451A CN106214958A CN 106214958 A CN106214958 A CN 106214958A CN 201610724451 A CN201610724451 A CN 201610724451A CN 106214958 A CN106214958 A CN 106214958A
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- slow down
- chicken
- down aging
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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Abstract
The invention provides health beverage of a kind of slow down aging and preparation method thereof, be made up of Radix Rehmanniae Preparata, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Folium Ilicis, Rhizoma Polygonati, chicken and duck embryo extract；This beverage has effect of kidney tonifying, essence replenishing, strengthening spleen and tonifying lung, Yin-nourishing and body fluid promoting, test proves have the function significantly regulating immunity of organisms, slow down aging, and the multiple medical material that the present invention selects is integration of edible and medicinal herbs, safe without toxic side effect, it is especially suitable for industrialized great production.
Description
Technical field
The invention belongs to technical field of Chinese medicines, particularly relate to health beverage of a kind of slow down aging and preparation method thereof.
Background technology
The mankind explore the reason of aging and pursue " alive for evermore " long-cherished wish that always mankind are fine from ancient times to the present, and study
Think old and feeble to a great extent by genetic control and there is certain program, though people can not " alive for evermore ", but after aging is also subject to
My god, the impact of environment and there is change at random or in advance or accelerate, thus show individual variation.
Chinese medicine thinks that the growth of human body, growth and aging are closely related with " kidney ".The Tang Dynasty Sun Simiao is at the age of one hundred years old
Famous doctor, he thinks that senile pathogenesis is mainly deficiency of kidney-essence, and opinion the kidney invigorating adds the medicine of essence with slow down aging.He
" a health preserving piece " lists Radix Rehmanniae, Rhizoma Polygonati, Semen Pini, Bai Shi, Poria, boxthorn root etc. as health preserving folk prescription.From successive dynasties slow down aging
Formula analysis, accounts for 70% with the kidney invigorating and essence nourishing for main formula person；Mid-aged population carries out determining the pathogenesis of ZANG-FU disease based on the differentiation of symptoms and signs, and also observing suffers from a deficiency of the kidney accounts for
80.4%, illustrate that deficiency of kidney-essence really accounts for critical role in aging.Therefore, the slow down aging prescription applied in recent years occupies with the kidney invigorating
Most.The traditional Chinese medical science is thought, " the congenital foundation is at kidney ", " the foundation of acquired constitution being the spleen "."Nei Jing" is said: " the five internal organs all report gas in stomach, stomach person, and five
Dirty basis is also ", gold dollar doctor's Li Dong Yuan is according to " taste are the vim and vigour root of YIN and YANG bases of a fruit ", it is believed that " taste both and, its life insurance；Taste
Discord, its people dies young ".The good health and a long life of human body depend on the normal of vital organs of the human body function, and the latter depends on that QI of the spleen and stomach is contained
Declining, the strong fortune of taste, the five internal organs are improved oneself；Taste are decayed, and the five internal organs are then hindered.It is important that visible taste essence of water and grain is supported in life lengthening
Property.
Modern medicine study shows, " kidney " alleged by the traditional Chinese medical science has " hypothalamo-pituitary-adrenal axis " and " inferior colliculus
Brain-Pituitary-Thyroid Axis " function, i.e. close with the neuroendocrine of modern medicine and immune relationship.Hypophysis is regulation
Immunoreactive major organs, by secreting the effect of ACTH, promotes acth secretion corticosteroid to rise and reduce or suppression
Too high immunoreation, hypophysis is by effects such as growth hormone simultaneously, excites immunity, strengthens or improve too low immunity anti-
Should, thus reach to adjust immunologic balance, maintain the metastable effect of immunity of organism basic function.Zoopery and clinical sight
Examining confirmation, kidney-replenishing can improve interrenal system irritability, and tonifying kidney-yin can stablize this irritability, illustrates to pass through the kidney invigorating
Cloudy, kidney-replenishing regulation immune function of human body." healthy energy " of motherland's medical science has immune normal function and structure." healthy energy "
In close relations with kidney, spleen, lung, kidney controlling essence storage, for the congenital foundation；The spleen has the function to transport and transform nutrients, for source of generating QI and blood；The lung is concerned with QI of the whole body,
Play compress and subsidy effect.
Visible successive dynasties physician attaches great importance to by the kidney invigorating, spleen invigorating, the treatment of medicine of lung benefiting, and the kidney invigorating, spleen invigorating, lung benefiting are to work as
Front enhancing human body immunity and the main rule for the treatment of of slow down aging, enhancing human body immunity function, to reach the purpose of life lengthening.
Summary of the invention:
The present invention is based on traditional theory of Chinese medical science, using the kidney invigorating, spleen invigorating, lung benefiting as enhancing human body immunity and slow down aging
The main rule for the treatment of, filters out kidney tonifying, essence replenishing, strengthening spleen and tonifying lung, the Chinese medicine of Yin-nourishing and body fluid promoting carry out prescription, it is provided that a kind of can enhancing body
Immunity, the Chinese medicinal health-care drink of slow down aging, its concrete scheme is as follows:
A kind of health beverage of slow down aging, by weight, it is characterised in that: by Radix Rehmanniae Preparata 50-200 part, Fructus Corni
50-200 part, Cortex Moutan 25-100 part, Rhizoma Dioscoreae 25-100 part, Poria 25-100 part, Rhizoma Alismatis 25-100 part, Folium Ilicis 12-50 part,
Rhizoma Polygonati 12-50 part, chicken and duck embryo extract 50-200 part are that active ingredient composition is made；
More specifically, the health beverage of a kind of slow down aging, by weight, it is characterised in that: Radix Rehmanniae Preparata 100 parts, mountain
The Fructus Evodiae 100 parts, Cortex Moutan 50 parts, Rhizoma Dioscoreae 50 parts, 50 parts of Poria, Rhizoma Alismatis 50 parts, Folium Ilicis 25 parts, Rhizoma Polygonati 25 parts, chicken and duck embryo
Extract 100 parts is made for active ingredient composition；
Described chicken and duck embryo extract is made up of following steps: take the wine that the chicken and duck hatching egg of 8-15 days is with 75%
Fine purifiation is clean, breaks eggshell into pieces, takes out embryo, removes allantois, shreds, and cleans with the D-Hanks liquid dual anti-containing high concentration, then uses
DMEM culture medium is cleaned；After cleaning, embryo is put in even oar machine even oar under the conditions of 4-10 DEG C；Take out even oar, add the excess of imports
Pure water and PH buffer, be centrifuged under the conditions of 4-10 DEG C separating, and after being centrifuged, Aspirate supernatant, by cold for supernatant 1-4 DEG C
Hide and stand, filter, fixed the most molten, degerming after embryo extract put into-80 DEG C at lyophilization, by cold at dry extract-20 DEG C
Freeze standby.
Active ingredient composition of the present invention is in use, can be mixing of directly pulverizing, it is also possible to be by
Chinese crude drug extracts active component through this area conventional extraction techniques, mixes with chicken and duck embryo extract, then with pharmaceutically may be used
The pharmaceutic adjuvant mixing accepted, makes required health product.
Present invention also offers the preparation method of the health beverage of a kind of slow down aging, comprise the following steps:
Take the Radix Rehmanniae Preparata of formula ratio, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Folium Ilicis, Rhizoma Polygonati, add medicine 8-
The water of 12 times amount, soaks 30-50min, heated and boiled 30-40min, filters, and medicinal residues are abandoned out, and filtrate is heated to 80 DEG C, add institute
The chicken and duck embryo extract of the amount of stating, stirring, to being completely dissolved, adds sweeting agent seasoning, again filters, sterilizing, and bottling to obtain final product
The health beverage of slow down aging.
Sweeting agent of the present invention can be selected from glucose, fructose, sucrose, maltose, starch sugar and lactose
Plant or multiple mixing.
The invention have the benefit that
Radix Rehmanniae Preparata: sweet in the mouth；Warm in nature；Return liver；Kidney channel；Enrich blood moist；Benefit essence fills out marrow, for blood deficiency and yellow complexion；Dizziness cardiopalmus；Month
Through uncomfortable；Collapse more than；Hepatic and renal YIN deficiency；Hectic fever night sweat；Seminal emission sexual impotence；Infertility；Menoxenia；Metrostaxis；Soreness of the waist and knees；
Hiccough and deaf；The head is dim-sighted；Early whitening of beard and hair；Quench one's thirst；Constipation；Suffer from a deficiency of the kidney dyspnea with rapid and short breath.
Fructus Corni: return Liver Channel；Kidney channel.Liver and kidney tonifying；Convergence is solid de-.Cure mainly: waist is had a dizzy spell；Deafness and tinnitus；The acid of waist knee joint
Soft；Xian essence spermatorrhea；Frequent micturition；Incessant sweating due to debility；Woman uterine bleeding.
Cortex Moutan: nature and flavor: arduous；Cool；It is slightly cold.GUIXIN warp；Liver Channel；Kidney channel；Lung meridian.Function: heat clearing away；Promoting blood circulation to remove blood stasis.
Rhizoma Dioscoreae: sweet in the mouth, property is put down.Return spleen, lung, kidney channel.For spleen reinforcing nourishing the stomach, promote the production of body fluid lung benefiting, the kidney invigorating arresting seminal emission.
Poria: nature and flavor: sweet, light is flat.GUIXIN warp, lung meridian, spleen channel, kidney channel；Dysuria；Edema distension；Phlegm retention cough with dyspnea；
Vomiting；Insufficiency of the spleen lack of appetite；Have loose bowels；Palpitation and restlessness；Insomnia forgetfulness；Seminal emission nebulousurine.
Rhizoma Alismatis: sweet in the mouth；Light；Cold in nature；Return kidney；Bladder warp.Function cures mainly: promoting diuresis to eliminate damp pathogen；Expel the heat-evil treating stranguria.Main dysuria；
The puckery pain of pyretic stranguria；Edema distension；Have loose bowels；Dizziness due to fluid-retention；Seminal emission.
Folium Ilicis: nature and flavor, sweet；Bitter；Cold；Gui Jing, enters liver, lung, stomach, for wind and heat dispersing；Improving eyesight is promoted the production of body fluid.
Rhizoma Polygonati: sweet in the mouth, property is put down.Energy nourishing kidney lung moistening, invigorating the spleen and replenishing QI.
Chicken and duck embryo extract: there is treatment headache, migraine, the effect of strengthening the spleen and stomach, then play the merit of building body
Effect, rich in free amino acid and multiple bio-hormone, especially embryo liquid, rich in active ecological nutrient, is easily human body
Absorbed, body immunity and resistance can be strengthened, nourished skin care, health body-building.
The present invention, based on traditional theory of Chinese medical science, cures mainly principle from kidney tonifying, spleen invigorating, lung benefiting, soothing the liver conduct, through substantial amounts of
Screening test, filters out the Chinese medicine of kidney tonifying, essence replenishing, strengthening spleen and tonifying lung, Yin-nourishing and body fluid promoting, and the pharmaceutical composition of the present invention is through function test
Prove that there is the function significantly regulating immunity of organisms, slow down aging, test result indicate that pharmaceutical composition of the present invention can promote
Enter the formation of hemolysin, strengthen mouse DTH and the ability of carbonic clearance, improve serum activity of SOD, reduce serum MDA and rats'liver
The content of lipofuscin in dirty tissue, extends life span of drosophila melanogaster, and the multiple medical material that the present invention selects is integration of edible and medicinal herbs, safety non-toxic
Side effect.
For can preferably illustrate that beneficial effects of the present invention, the present invention have also listed following test example, it is intended to this is described
Bright, but it is by no means limited to protection scope of the present invention.
One, pharmacodynamic study
Test example 1: the effective ingredient compositions enhancing immunity experimentation of the present invention
1, material
1.1 medicine
Test group 1: mouse stomach is administered, and gives Radix Rehmanniae Preparata 100g, Fructus Corni 100g, Cortex Moutan 50g, Rhizoma Dioscoreae 50g, Poria
The Chinese medicine composition that 50g, Rhizoma Alismatis 50g make；
Test group 2: mouse stomach is administered, and gives Radix Rehmanniae Preparata 100g, Fructus Corni 100g, Cortex Moutan 50g, Rhizoma Dioscoreae 50g, Poria
The pharmaceutical composition that 50g, Rhizoma Alismatis 50g, Rhizoma Polygonati 50g, Folium Ilicis 50g make；
Test group 3: mouse stomach is administered；Give Radix Rehmanniae Preparata 100g, Fructus Corni 200g, Cortex Moutan 50g, Rhizoma Dioscoreae 100g, Fu
The pharmaceutical composition that Siberian cocklebur 50g, Rhizoma Alismatis 100g, Rhizoma Polygonati 25g, Folium Ilicis 25g make；
Test group 4: mouse stomach is administered；Give chicken and duck embryo extract 50g/kg body weight.
Test group 5: mouse stomach is administered；Give Radix Rehmanniae Preparata, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Rhizoma Polygonati, hardship
Fourth tea, chicken and duck extract, the pharmaceutical composition made by the formula of embodiment 1-3 respectively, numbered test group sample 5-1,5-
2、5-3；
Positive controls: mouse stomach is administered, Nidus collocaliae 50g/kg body weight.
Blank group: distilled water, mouse stomach is administered.
1.2 animals: Healthy female ICR mice, body weight 18-22g, test mice original body mass equilibrium between each group.
2, experimental technique
Select healthy mice, each Testing index to set mice 108, be divided into 9 groups, often group 12.Each group mice is all pressed on an equal basis
Dosage continuous per os gavage, blank group gavage equal-volume distilled water, after 30d, survey every immune indexes.
2.1 spleens/body weight ratio and antibody-producting cell test experience
Mice organs/body weight ratios affect experiment: mice organs injection SRBC, puts to death animal after 5d, take thymus, spleen claims
Weight, calculates internal organs/body weight ratio.Prepare splenocyte suspension simultaneously, carry out antibody-producting cell mensuration.
Mouse antibodies cellulation test experience: take spleen, make cell suspension.By after the culture medium heating for dissolving of top layer with etc.
Measure the mixing of double Hanks liquid, subpackage small test tube, often pipe 0.5ml, then (v/v joins with SA liquid to add 50 μ L10%SRBC in pipe
System), 20 μ L splenocyte suspensions, rapidly after mixing, be poured on the slide of brush agarose thin layer, after agar solidification, will be broken
Sheet level button is placed on slide frame, puts into CO2Incubator incubation 1.5h, then joins glass with the complement (1:8) of SA liquid dilution
In horse groove, after continuing incubation 1.5h, count hemolysis plaque number.
2.2 mouse spleen lymphocyte transformation experiments
Aseptic take spleen, prepare splenocyte suspension, wash 2 times with Hanks liquid, be centrifuged 10min (1000r/min) every time, will be thin
Born of the same parents are suspended in 1mL RPMI1640 complete culture solution, and adjusting cell concentration is 3 × 106/mL.Cell suspension point holes is added
Entering in 24 well culture plates, every hole 1mL, a hole adds 75 μ L ConA liquid, and 5%CO, as comparison, is put in another hole2, 37 DEG C of incubators
Middle cultivation 72h.Cultivation terminates front 4h, every hole Aspirate supernatant 0.7mL, adds the 0.7mL RPMI1640 without calf serum complete
Full culture fluid, is simultaneously introduced MTT50 μ L, continues to cultivate 4h.After cultivation terminates, every hole adds 1mL acid isopropyl alcohol, and piping and druming is all
Even, make purple crystal be completely dissolved, at 570nm wavelength, measure OD value, deduct and be not added with ConA hole adding the OD value in ConA hole
OD value represents lymphopoiesis ability.
2.3 mice delayed allergy experiments
Mouse peritoneal injection 2% (v/v) SRBC, after sensitization, 4d measures left back sufficient sole of the foot thickness, then subcutaneous in measuring point
Injecting 20% (v/v) SRBC, every Mus injects 20 μ L, and after injection, 24h measures left back sufficient sole of the foot portion thickness, and same position is measured 3 times, takes
Average.The degree of DTH is represented with foot sole of the foot thickness difference (swelling degree of the paw) before and after attacking.
2.4 mouse antibodies cellulation test experience
Take spleen, make cell suspension.Being mixed by Hanks liquid double with equivalent after the culture medium heating for dissolving of top layer, subpackage is little
Test tube, often pipe 0.5ml, then in pipe, add 50 μ L 10%SRBC (v/v prepares), 20 μ L splenocyte suspensions with SA liquid, mix rapidly
After even, it is poured on the slide of brush agarose thin layer, after agar solidification, fragmentation level button is placed on slide frame, puts into
CO2Incubator incubation 1.5h, then joins in slide frame groove with the complement (1:8) of SA liquid dilution, after continuing incubation 1.5h,
Counting hemolysis plaque number.
2.5 Mouse Bloods purify the blood lysin test experience
Taking Sanguis caprae seu ovis, brine 3 times, the most centrifugal (2000r/min) 10min, every Mus is pressed through lumbar injection 2%
Long-pending SRBC0.2mL carries out immunity.After 4d, extract eyeball and take blood in centrifuge tube, place about 1h, solidification blood is peeled off with tube wall,
Making serum fully analyze, 2000r/min is centrifuged 10min, collects serum.With SA buffer by serum-dilution.After dilution
Serum 1mL puts in test tube, is sequentially added into 10% (V/V) SRBC0.5mL, complement 1mL.Separately set the control tube of not increase serum (with SA
Buffer replaces).Putting after being incubated 25min in 37 DEG C of waters bath with thermostatic control, ice bath terminates reaction.2000r/min is centrifuged 10min, takes
Clear 1mL, adds Dou Shi reagent 1mL.Take 10% (V/V) SRBC0.25mL simultaneously, add Dou Shi reagent to 4mL, fully mix, place
After 10min, sentence control tube in 540nm and make blank, survey each pipe optical density value respectively.The amount of hemolysin is with half hemolysis value
(HC50) represent.
2.6 mice carbonic clearance experiments
India ink to mouse tail vein injection 1:3 dilution, treats that prepared Chinese ink injects timing immediately, injects 10min after prepared Chinese ink,
Take blood 20 μ L from angular vein clump respectively, and be added in 2mL Na2CO3 solution, exist with ultraviolet-uisible spectrophotometer
600nm wavelength sentences Na2CO3Solution makees blank densitometric value (OD).By sacrifice, take liver and spleen is weighed, calculate
Phagocytic index.
2.7 NK cells in mice determinations of activity
Before experiment, 24h is by target cell Secondary Culture, washes 3 times with Hanks liquid before application, adjusts with RPMI1640 complete culture solution
Whole cell concentration is 4 × 105/mL.Mice cervical dislocation is put to death, and aseptic takes spleen, prepares splenocyte suspension, washes 2 with Hanks liquid
Secondary, it is centrifuged 10min (1000r/min) every time.Abandon supernatant cytoplasm to be upspring, add 0.5mL aquesterilisa splitting erythrocyte, 20s
After add 2 times of Hanks liquid of 0.5mL and 8mL Hanks liquid, centrifugal 10min (1000r/min), with 1mL containing 10% calf blood
Clear RPMI1640 complete culture solution is resuspended, counts after 1% glacial acetic acid dilution, and adjusting cell concentration is 2 × 107/mL.Will
Target cell adds 96 well culture plates, every hole 100 μ L, and test hole adds 100 μ L splenocytes (effect target is than 50:1), and Spontaneous release hole adds
Entering 100 μ L culture fluid, maximum release aperture adds 100 μ L 1%NP40, cultivates 4h for 37 DEG C, centrifugal, takes supernatant 100 μ L and puts 96 hole enzymes
In target, add LDH matrix liquid 100 μ L, react 6min, terminate reaction with the HCL of 1mol/L, measure at microplate reader 490nm
Value OD value.
3, experimental result
3.1 body weight change situations
Before testing, all mices all weigh body weight value；Mice accepts after tested material 30 days, and all mices the most all claim
Taking body weight value, because mice original body mass equalizes, after accepting tested material, body weight is also without significant difference, test group 1-12 and positive control
Group compare with blank group, P > 0.05, thus invention formulation on Mouse Weight without affect.
3.2 immunity measurement results
According to " health-care food evaluation and technical specification " relevant criterion, now experiment acquired results is divided into: internal organs/body weight
Ratio measurement, comprises spleen/body weight ratio, thymus/body weight ratio, the results are shown in Table 1；Cellular immune function assay, comprises little
The impact of Mus Splenic vein hemodynamics is tested, is tested mice delayed allergy, the results are shown in Table 2；Humoral immune function measures,
Comprise mouse antibodies cellulation test experience, Mouse Blood purifies the blood lysin test experience, the results are shown in Table 3；Monocytes/macrophages merit
Can measure, comprise the impact test of mice carbonic clearance, Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell test, the results are shown in Table 4；NK
Cytoactive detection, the results are shown in Table 5.
The impact on mice organs/body weight ratio of each group of the table 1
| Group | Spleen/body weight ratio (× 102) | Thymus/body weight ratio (× 102) |
| Test group 1 | 0.40±0.08 | 0.35±0.03 |
| Test group 2 | 0.46±0.06 | 0.34±0.04 |
| Test group 3 | 0.42±0.03 | 0.34±0.03 |
| Test group 4 | 0.42±0.04 | 0.33±0.03 |
| Test group 5 | 0.42±0.01 | 0.31±0.04 |
| Positive controls | 0.42±0.06 | 0.33±0.04 |
| Blank group | 0.42±0.02 | 0.33±0.05 |
Result: each group of impact on mouse spleen/body weight ratio: each test group and positive controls and blank group ratio
Relatively, P > 0.05；The each group of impact on mouse thymus/body weight ratio: test group 1-5 and positive controls and blank group ratio
Relatively, P > 0.05；Conclusion: the pharmaceutical composition of test group 1-5 and positive controls on mice organs/body weight ratio without impact.
Each group of table 2 is to mouse cell immunity function result
| Group | Lymphocyte proliferation ability (OD difference) | Swelling degree of the paw |
| Test group 1 | 0.48±0.06 | 0.52mm±0.04mm |
| Test group 2 | 0.57±0.04 | 0.54mm±0.05mm |
| Test group 3 | 0.62±0.06 | 0.55mm±0.08mm |
| Test group 4 | 0.63±0.06 | 0.52mm±0.06mm |
| Test group 5 | 0.73±0.09 | 0.71mm±0.09mm |
| Positive controls | 0.67±0.04 | 0.63mm±0.07mm |
| Blank group | 0.10±0.03 | 0.31mm±0.03mm |
Remarks: lymphocyte proliferation ability: test group 1-5 and positive controls compare with blank group, P ＜ 0.05；
Swelling degree of the paw: test group 1-5 and positive controls compare with blank group, P ＜ 0.05；
Conclusion: mouse cell immunity test result is positive by each group of this test and positive controls, test group 5 effect is
Excellent, and it is better than positive controls, the therefore preferred Radix Rehmanniae Preparata of the present invention, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Huang
Essence, Folium Ilicis, chicken and duck extractive composition formula proportion be effect highly significant in terms of strengthening mouse cell immunologic function.
Each group of table 3 is to mouse humoral immune function result
| Group | Hemolysis plaque number (× 103/ full spleen) | Half hemolysis value (HC50) |
| Test group 1 | 230±31 | 58±22 |
| Test group 2 | 232±26 | 59±22 |
| Test group 3 | 235±35 | 60±21 |
| Test group 4 | 237±35 | 61±22 |
| Test group 5 | 263±37 | 90±26 |
| Positive controls | 250±32 | 72±18 |
| Blank group | 146±16 | 48±17 |
Remarks: hemolysis plaque number: test group 1-5 of the present invention compares with blank group, P ＜ 0.05；
Half hemolysis value: test group 1-5 of the present invention compares with blank group, P ＜ 0.05.
Conclusion: mouse humoral immune function test result is positive by each group of this test, test group 5 effect is optimum, and
It is better than positive controls, therefore the preferred Radix Rehmanniae Preparata of the present invention, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Rhizoma Polygonati, Herba Lactucae formosanae
Tea, chicken and duck extractive composition formula proportion be effect highly significant in terms of strengthening mouse cell immunologic function.
Each group of table 4 affects result to mouse monokaryon-macrophage function
Remarks: carbonic clearance ability phagocytic index: test group 1-5 of the present invention compares with blank group, P ＜ 0.05；Half
Haemolysis value: phagocytosis chicken red blood cell ability phagocytic percentage compares with blank group with phagocytic index test group 1-5, P ＜ 0.05；
Conclusion: mouse monokaryon-macrophage function result of the test is positive by each group of this test, test group 5 effect is optimum,
And be better than positive controls, therefore the preferred Radix Rehmanniae Preparata of the present invention, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Rhizoma Polygonati,
Folium Ilicis, chicken and duck extractive composition formula proportion be effect highly significant in terms of strengthening mouse cell immunologic function.
Each group of table 5 affects result to NK cells in mice activity
Result: test group 1-5 of the present invention compares with blank group, P ＜ 0.05；
Conclusion: each group of this test affects result of the test to Mice Mice NK cytoactive and is positive, and each group of this test is to little
Mus monocytes/macrophages function test result is positive, and test group 5 effect is optimum, and is better than positive controls, therefore originally
Invent preferred Radix Rehmanniae Preparata, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Rhizoma Polygonati, Folium Ilicis, chicken and duck extract combination composition formula
Effect highly significant in terms of strengthening mouse cell immunologic function.
Totally test result indicate that, preferred compound composition of the present invention can improve the splenocyte of mice and increase
Grow ability, increase antibody-producting cell number, raising serum hemolysin, enhancing Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell
Ability, enhancing mouse monokaryon-macrophage phagocytic function, enhancing NK cells in mice activity；To spleen/body weight ratio, thymus/body
Anharmonic ratio value, swelling degree of the paw are without impact；Illustrate that the action effect of the present invention effective formula proportion compositions enhancing immunity shows
Write.
Test example 2: the Chinese medicine composition slow down aging experimentation of the present invention
1, drosophila survival experiment
1.1 substances and administering mode: with content under the 1.1-medicine item of test example 1.
1.2 experimental animals: Oregon K wild type Drosophila melanogaster (Drosophila melanogaster)
1.3 dosage packets: except blank group, the concentration difference equal 0.4% of each group culture medium pastille.
1.4 test methods: the new fruit bat adult sprouted wings in collecting 8 hours, distinguish under etherization male and female random packet,
Test after weighing respectively.Often group fruit bat 400, male and female half and half.Blank group gives conventional corn powder culture medium；Examination
Test group and give the culture medium containing 0.4% medicine respectively.
1.5 experiment conditions: temperature 25 ± 10 DEG C, relative humidity 45-75%.Within every four days, change fresh culture once.Often
It observed and recorded drosophila survival number and death toll, until fruit bat all death.Calculate half death natural law, average life
With three indexs such as Mean longest lifes.Result such as table 6 below:
Table 6: drosophila survival experiment result
As seen from the above table, under this experiment condition, test group 5 of the present invention delays drosophila survival situation optimum, relatively blank
Matched group, extends average life 4.3-4.7 days.The preferred Radix Rehmanniae Preparata of the present invention, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, pool are described
Rush down, Rhizoma Polygonati, Folium Ilicis, chicken and duck extractive composition formula proportion application effect in slow down aging the most notable.
2, the Chinese medicinal drink that the present invention the prepares research to slow down aging:
2.1 experimental animals: Oregon K wild type Drosophila melanogaster (Drosophila melanogaster)
1.3 administrations: test group: give the present invention and prepare beverage, matched group: give commercially available Nidus collocaliae.
1.4 test methods: the new fruit bat adult sprouted wings in collecting 8 hours, distinguish under etherization male and female random packet,
Test after weighing respectively.Often group fruit bat 400, male and female half and half.Test group and matched group give respectively containing 0.6% medicine
Culture medium.
1.5 experiment conditions: temperature 25 ± 10 DEG C, relative humidity 45-75%.Within every four days, change fresh culture once.Often
It observed and recorded drosophila survival number and death toll, until fruit bat all death.Calculate half death natural law, average life
With three indexs such as Mean longest lifes.
Illustrate that the present invention is more excellent relative to commercially available Nidus collocaliae effect in delaying senility function.
In sum, the health-care Chinese medicinal composition of a kind of slow down aging that the present invention provides and raw material thereof, to strengthening immunity
Power and slow down aging drug effect highly significant, and very production cost is low, is especially suitable for industrialized production.
Detailed description of the invention:
Embodiment 1: the preparation of the health care Chinese medicine beverage of a kind of slow down aging:
Formula: Radix Rehmanniae Preparata 50g, Fructus Corni 200g, Cortex Moutan 25g, Rhizoma Dioscoreae 100g, Poria 100g, Rhizoma Alismatis 25g, Folium Ilicis
25g, Rhizoma Polygonati 100g, chicken and duck embryo extract 50g；
Preparation method:
Take the Radix Rehmanniae Preparata of above-mentioned weight portion meter, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Folium Ilicis, Rhizoma Polygonati add and are used as medicine
The water of thing 8 times amount, soaks 50min, heated and boiled 40min, filters, and medicinal residues are abandoned out, and filtrate is heated to 80 DEG C, adds described amount
Chicken and duck embryo extract, stirring, to being completely dissolved, adds appropriate sweeting agent seasoning, again filters, sterilizing, and bottling to obtain final product
The health beverage of slow down aging.
Wherein the preparation process of chicken and duck embryo extract is as follows: takes the chicken and duck hatching egg of 8 days and cleans with the ethanol of 75%, strikes
Scrambled egg shell, takes out embryo, removes allantois, shreds, and cleans with the D-Hanks liquid dual anti-containing high concentration, then uses DMEM culture medium
Clean；After cleaning, embryo is put in even oar machine even oar under the conditions of 4 DEG C；Take out even oar, add ultra-pure water and PH buffering
Liquid, is centrifuged under the conditions of 4 DEG C separating, after being centrifuged, and Aspirate supernatant, 1 DEG C of cold preservation of supernatant is stood, filters, fixed molten,
Embryo extract after degerming puts into lyophilization at-80 DEG C, by freezing standby at dry extract-20 DEG C.
Embodiment 2: the preparation of the health care Chinese medicine beverage of a kind of slow down aging:
Formula: Radix Rehmanniae Preparata 100g, Fructus Corni 100g, Cortex Moutan 50g, Rhizoma Dioscoreae 50g, Poria 50g, Rhizoma Alismatis 50g, Folium Ilicis
25g, Rhizoma Polygonati 25g, chicken and duck embryo extract 100g；
Preparation method:
Take the Radix Rehmanniae Preparata of above-mentioned weight portion meter, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Folium Ilicis, Rhizoma Polygonati add and are used as medicine
The water of thing 10 times amount, soaks 40min, heated and boiled 40min, filters, and medicinal residues are abandoned out, and filtrate is heated to 80 DEG C, adds described amount
Chicken and duck embryo extract, stirring to being completely dissolved, add appropriate sucrose seasoning, again filter, sterilizing, bottling, to obtain final product
The health beverage of slow down aging.
Wherein the preparation process of chicken and duck embryo extract is as follows: takes the chicken and duck hatching egg of 12 days and cleans with the ethanol of 75%,
Break eggshell into pieces, take out embryo, remove allantois, shred, clean with the D-Hanks liquid dual anti-containing high concentration, then cultivate with DMEM
Base cleans；After cleaning, embryo is put in even oar machine even oar under the conditions of 7 DEG C；Take out even oar, add ultra-pure water and PH delays
Rush liquid, be centrifuged under the conditions of 7 DEG C separating, after being centrifuged, Aspirate supernatant, 3 DEG C of cold preservations of supernatant are stood.Filter, fixed
Molten, degerming after embryo extract put into-80 DEG C at lyophilization, by freezing standby at dry extract-20 DEG C.
Embodiment 3: the preparation of the health care Chinese medicine beverage of a kind of slow down aging:
Formula: Radix Rehmanniae Preparata 200g, Fructus Corni 50g, Cortex Moutan 100g, Rhizoma Dioscoreae 25g, Poria 25g, Rhizoma Alismatis 100g, Folium Ilicis
100g, Rhizoma Polygonati 25g, chicken and duck embryo extract 200g；
Preparation method:
Take the Radix Rehmanniae Preparata of above-mentioned weight portion meter, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Folium Ilicis, Rhizoma Polygonati add and are used as medicine
The water of thing 12 times amount, soaks 30min, heated and boiled 30min, filters, and medicinal residues are abandoned out, and filtrate is heated to 80 DEG C, adds described amount
Chicken and duck embryo extract, stirring to being completely dissolved, add appropriate lactose seasoning, again filter, sterilizing, bottling, to obtain final product
The health beverage of slow down aging.
Wherein the preparation process of chicken and duck embryo extract is as follows: takes the chicken and duck hatching egg of 15 days and cleans with the ethanol of 75%,
Break eggshell into pieces, take out embryo, remove allantois, shred, clean with the D-Hanks liquid dual anti-containing high concentration, then cultivate with DMEM
Base cleans；After cleaning, embryo is put in even oar machine even oar under the conditions of 10 DEG C；Take out even oar, add ultra-pure water and PH delays
Rush liquid, be centrifuged under the conditions of 10 DEG C separating, after being centrifuged, Aspirate supernatant, 4 DEG C of cold preservations of supernatant are stood, filters, fixed
Molten, degerming after embryo extract put into-80 DEG C at lyophilization, by freezing standby at dry extract-20 DEG C.
Claims (5)
1. a health beverage for slow down aging, by weight, it is characterised in that: by Radix Rehmanniae Preparata 50-200 part, Fructus Corni 50-
200 parts, Cortex Moutan 25-100 part, Rhizoma Dioscoreae 25-100 part, Poria 25-100 part, Rhizoma Alismatis 25-100 part, Folium Ilicis 12-50 part, Huang
Essence 12-50 part, chicken and duck embryo extract 50-200 part are that active ingredient composition is made.
The health beverage of a kind of slow down aging the most according to claim 1, by weight, it is characterised in that: by Radix Rehmanniae Preparata
Huang 100 parts, Fructus Corni 100 parts, Cortex Moutan 50 parts, Rhizoma Dioscoreae 50 parts, 50 parts of Poria, Rhizoma Alismatis 50 parts, Folium Ilicis 25 parts, Rhizoma Polygonati 25
Part, chicken and duck embryo extract 100 parts are made for active ingredient composition.
The health beverage of a kind of slow down aging the most according to claim 1 and 2, it is characterised in that: described chicken and duck embryo
Extract is made up of following steps: takes the chicken and duck hatching egg of 8-15 days and cleans with the ethanol of 75%, breaks eggshell into pieces, take out embryo
Tire, removes allantois, shreds, and cleans with the D-Hanks liquid dual anti-containing high concentration, then cleans by DMEM culture medium, cleans complete
After embryo put in even oar machine even oar under the conditions of 4-10 DEG C；Take out even oar, add ultra-pure water and PH buffer, 4-10 DEG C of bar
Be centrifuged under part separating, centrifugal after, Aspirate supernatant, 1-4 DEG C of cold preservation of supernatant is stood, filters, fixed molten, degerming after
Embryo extract put into-80 DEG C at lyophilization, by freezing standby at dry extract-20 DEG C.
The health beverage of a kind of slow down aging the most according to claim 1 and 2, it is characterised in that: described effective ingredient
Compositions is in use, can be mixing of directly pulverizing, it is also possible to be through this area conventional extraction techniques by Chinese crude drug
Extract active component, mix with chicken and duck embryo extract, then mix with pharmaceutically acceptable pharmaceutic adjuvant, make required guarantor
Strong product.
5. the preparation method of the health beverage of a slow down aging, it is characterised in that: comprise the following steps:
Take the Radix Rehmanniae Preparata of formula ratio, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria, Rhizoma Alismatis, Folium Ilicis, Rhizoma Polygonati, add medicine 8-12 times
The water of amount, soaks 30-50min, heated and boiled 30-40min, filters, and medicinal residues are abandoned out, and filtrate is heated to 80 DEG C, add formula ratio
Chicken and duck embryo extract, stirring to being completely dissolved, add sweeting agent seasoning, again filter, sterilizing, bottling, must delay
Old and feeble health beverage.
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