CN106212048B - Silkworm chrysalis Cordyceps sinensis automatic vaccination equipment and its inoculation breeding method - Google Patents
Silkworm chrysalis Cordyceps sinensis automatic vaccination equipment and its inoculation breeding method Download PDFInfo
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- CN106212048B CN106212048B CN201610610671.9A CN201610610671A CN106212048B CN 106212048 B CN106212048 B CN 106212048B CN 201610610671 A CN201610610671 A CN 201610610671A CN 106212048 B CN106212048 B CN 106212048B
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 91
- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 42
- 238000002255 vaccination Methods 0.000 title claims abstract description 24
- 238000011081 inoculation Methods 0.000 title description 15
- 238000009395 breeding Methods 0.000 title description 4
- 230000005540 biological transmission Effects 0.000 claims abstract description 50
- 230000001105 regulatory effect Effects 0.000 claims abstract description 10
- 239000000523 sample Substances 0.000 claims abstract description 9
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 238000003306 harvesting Methods 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 5
- 230000033228 biological regulation Effects 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 239000003638 chemical reducing agent Substances 0.000 abstract description 2
- 239000007921 spray Substances 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 241000190633 Cordyceps Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000003028 elevating effect Effects 0.000 description 3
- 240000001307 Myosotis scorpioides Species 0.000 description 2
- 241000382353 Pupa Species 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 244000283482 Alloteropsis cimicina Species 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000252254 Catostomidae Species 0.000 description 1
- 241001264174 Cordyceps militaris Species 0.000 description 1
- 235000017898 Digitaria ciliaris Nutrition 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
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- 230000001093 anti-cancer Effects 0.000 description 1
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- 210000003056 antler Anatomy 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
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- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A kind of silkworm chrysalis Cordyceps sinensis automatic vaccination equipment, including automatic injector, transmission device, retarder, speed regulating motor and silkworm chrysalis transmission device, electric machine main shaft is connect with retarder, reducer output shaft is connected with transmission device, transmission device is equipped with automatic injector, silkworm chrysalis transmission device is mounted on the lower section of automatic injector, speed probe is installed on silkworm chrysalis transmission device, position sensor is installed on automatic injector, speed probe and position sensor are connected with controller input terminal respectively, and controller output end is connected with speed regulating motor and automatic injector respectively.
Description
Technical field
The present invention relates to cordyceps sinensis to be inoculated with field, in particular to a kind of silkworm chrysalis Cordyceps sinensis automatic vaccination equipment.
Background technique
Cordyceps sinensis abbreviation cordyceps sinensis is precious Chinese medicine, it and ginseng, pilose antler are usually classified as three big tonics by people,
It is expensive, " soft gold " is described as by people.The effect of approving cordyceps sinensis with more and more people, market is to cordyceps sinensis
Demand it is increasing, supply falls short of demand more seems rare for cordyceps sinensis.It is rare in view of Cordyceps Resources, and excavate winter worm
Summer grass can damage environment, research and development can artificial cultivation cordyceps sinensis become the task of top priority.Silkworm chrysalis Cordyceps sinensis (Cordceps
Militaris(L.) Link) the northern winter worm of alias or Cordceps militaris.Cordyceps militaris belongs to Ascomycotina (Ascomycotina), ergot
Cordycepps (Claviciptaceae), Cordyceps (Cordyceps) after being insect infection Chinese caterpillar fungus hypha, form answering for worm and mycelia
Zoarium, be it is a kind of there is invigorating the lung and the kidney, nourishing and fit keeping function, anticancer, antifatigue, anti-aging, improve the immunity of the human body and other effects, have pole
The precious medicinal fungi of high value.Its with it is unique prevent and cure diseases, nourishing and fit keeping function the effects of be increasingly subject to the welcome of consumer,
Gradually instead of wild Chinese caterpillar fungus, become the optimal path for meeting that the market demand and alleviation resource gradually decrease.
Since Hengxian County has more peasant household and enterprise to breed silkworms, the acquisition of silkworm chrysalis is also very convenient, local government and
There is a wish of volume production silkworm chrysalis Cordyceps sinensis in enterprise, but the method yield that traditional artificial infection is cultivated is too low, people be dedicated to as
What efficient, standardization, industrialization ground production silkworm chrysalis Cordyceps sinensis, there are also enterprise, scientific research institutions and individuals to silkworm chrysalis worm at present
Grass how studied by mechanical inoculation, is the related patents retrieved below:
Chinese patent 1, a kind of title: Cordceps militaris liquid inoculator;Application number: 201520600619.6;The applying date:
2015-08-06;Applicant: Liu Xiaohong;Address: 118001 (Kingsoft schools of Yuanbao District, Dandong City, Liaoning Province culture road 325
Area);A kind of abstract: Cordceps militaris liquid inoculator.It includes body, universal wheel, culture box, control valve, cultivates box cover, is fixed
Vacuum chuck, lifting vacuum chuck, atomizing disinfection apparatus and inoculation spray head, organism bottom are provided with universal wheel, the work on body
Make to be provided with multiple fixation type vacuum suckers in plane, the culture that conveyer belt transmission comes is provided on fixation type vacuum sucker
Box is cultivated and is provided with culture box cover on box, and the setting of lifting vacuum chuck is additionally provided with spraying above culture box cover, and on body
Chlorination equipment, inoculation spray head, inoculation spray head are connected with the control valve for controlling inoculum concentration.The utility model structure is simple, connects
Kind is high-efficient, and pollution is low, and inoculum concentration is certain, practical, can be avoided Cordceps militaris and is polluted.
Chinese patent 2, a kind of title: Cordceps militaris liquid inoculator;Application number: 201420414592.7 applyings date:
2014-07-26;Applicant: Jinan Zhan Chuan Science and Technology Ltd.;Address: 250100 Jinan City, Shandong Province Licheng District Hua Longlu
No. 509 ChuangXin Building Room 206;Abstract: a kind of Cordceps militaris liquid inoculator belongs to the industrialized production equipment of food, medical fungus.
Including rack, conveying device, positioning stop gauge, lifting device, opening and closing lid arrangement, classification inoculation apparatus, ventilation bactericidal unit.Conveying
Device is mounted on the rack, and conveyer belt is located at the two sides of conveying device.It positions stop gauge installation on a conveyor, uses cylinder
Drive stock stop lifting.Lifting device is made of culture box fixed mechanism and elevating mechanism, is trained after elevating mechanism drive is fixed
Feeding case assembly is gone up and down.Opening and closing lid arrangement is mounted on the rack, and rear lift-sliding machine is sucked by box cover is cultivated in vacuum chuck group
Structure carries out transverse shifting after first rising culture box.Classification inoculation apparatus is mounted on the rack, and elevating mechanism drives nozzle component lifting.
In inoculation station, it is equipped with blower and air filtration and purification apparatus and ultraviolet lamp tube.The utility model uses automatic vaccination, production effect
Rate is high, and kind amount is accurate, improves the quality stability of product.
Chinese patent 3, a kind of title: edible mushroom cultivating machine;Application number: 201310442444.6;Application number:
201310478301.0 the applying date: 2013-10-14;Address: 312467 Shaoxing, Zhejiang Province city, the villages permanently happy town Kan Yi, Shengzhou
Bank stream cross road 31;Abstract: a kind of edible mushroom cultivating machine belongs to machinery field.Including pedestal, and what is be set on the base beat
Holes mechanism, vaccination mechanism and control unit, wherein the perforating mechanism includes guide post, is slidably arranged in upper in guide post
Pressing plate and lower platen are equipped with spring, the top of top board and the piston rod phase of pressing plate push cylinder between top board and lower platen
Even, above-mentioned top board and lower platen are equipped with several for installing perforating needle through-hole;The vaccination mechanism includes hopper, feeding
Slot, briquetting, inoculation bar and several cylinders, above-mentioned each cylinder pass through solenoid valve respectively and are connected with control unit.Using above-mentioned
Edible mushroom cultivating machine, worker can carry out automatic punching, inoculation by take-off plate, hopper according to the automatic particle of control signal, feed
Material, whole process all use relay to control, fast and easy, and without human intervention, avoid bacterium bag contaminated.
As can be seen that existing be exclusively used in silkworm chrysalis Cordyceps sinensis, the i.e. inoculating facility of Cordceps militaris such as patent 1 from above-mentioned document
With patent 2, it is all made of under conveyer belt send the silkworm chrysalis equipped with culture box to inoculation spray head and is inoculated with, although can be to a certain degree
Raising production efficiency, but this production method the degree of automation is not high, continuously can not rapidly be produced, and do not examine
Consider the easily dead feature of the fragility of silkworm chrysalis, vertical inoculation spray head, can be because spray head move back completely in actual moving process
Silkworm chrysalis begins to mobile and silkworm chrysalis is caused to be scratched injury out, infection and it is dead.And edible mushroom cultivating machine described in patent 3, one
Aspect is not the dedicated inoculating facility for silkworm chrysalis Cordyceps sinensis, and on the other hand, the structure of this inoculation device is excessively complicated, common agriculture
Family is difficult to operate.
Summary of the invention
The object of the present invention is to provide a kind of equipment for silkworm chrysalis Cordyceps sinensis automatic vaccination and its inoculation breeding methods.
To solve the above problems, the technical solution adopted is that:
Silkworm chrysalis Cordyceps sinensis automatic vaccination equipment, including automatic injector, transmission device, retarder, speed regulating motor and silkworm chrysalis pass
Device is sent, electric machine main shaft is connect with retarder, and reducer output shaft is connected with transmission device, and transmission device is equipped with automatic injection
Device, silkworm chrysalis transmission device are mounted on the lower section of automatic injector, and speed probe is equipped on silkworm chrysalis transmission device, automatic injection
Position sensor is installed, speed probe and position sensor are connected with controller input terminal respectively, controller output on device
End is connected with speed regulating motor and automatic injector respectively.
Using above-mentioned silkworm chrysalis Cordyceps sinensis automatic vaccination equipment, the process of silkworm chrysalis Cordyceps sinensis automatic vaccination is as follows:
(1) silkworm chrysalis sorting and cleaning: rotten, rotten silkworm chrysalis is sub-elected, impurity is removed;
(2) automatic vaccination: the silkworm chrysalis after cleaning is put into silkworm chrysalis transmission device, is connect by silkworm chrysalis Cordyceps sinensis automatic vaccination equipment
Kind, every silkworm chrysalis inoculum concentration control is 2-5ml;
(3) sterile culture: the silkworm chrysalis for accomplishing fluently kind is cased with 50cmx30cm incubator and is sent into desinfection chamber culture 10-15
It;
(4) mycelia grows up: cordyceps sinensis cultured in desinfection chamber being put into and continues to cultivate in glass jar, and the 2-3 that sprays water daily
Secondary, mycelia growth later-stage utilization fluorescent lamp suitably gives illumination and accelerates growth.
(5) cordyceps sinensis harvests: cordyceps sinensis can harvest after cultivating 15-20 days in glass jar.
The transmission device includes cam, fifth wheel, fixed spring, connecting rod and telescopic rod, the output shaft of retarder
It is connect with cam, cam and fifth wheel are fixed by fixed spring, and fifth wheel is fixedly connected with the connecting rod, connecting rod and Vertical Square
To in 15-30 ° of angle, connecting rod leading portion is equipped with automatic injector.
The conveyor belt design of the silkworm chrysalis transmission device has the groove of 2-5cm wide, and a lattice groove is filled with a silkworm chrysalis, leads to
It crosses control conveyer belt transmission speed and is inoculated with the revolving speed realization automatic vaccination of cam in the device.
The automatic injector tilts 15-30 ° to silkworm chrysalis transmission device direction of advance, and automatic injector is avoided to scratch silkworm
Pupa causes silkworm chrysalis injured or dead.
The working principle of the invention:
When silkworm chrysalis transmission device send silkworm chrysalis to automatic injector, position sensor detects whether to arrive at recess, supports
When up to recess, transmission device knit stitch, automatic injector injects strain, when sensor, which detects, arrives at projection, position sensor hair
Signal out, automatic injector rise to original position, and controller control silkworm chrysalis transmission device continues to run, turns on silkworm chrysalis transmission device
Fast sensor monitors revolving speed in real time, and feeds back to controller and regulation is compared, and forms closed-loop control system, has Self-tuning System tune
Function is saved, there is incubator in the end of silkworm chrysalis transmission device, is sent into desinfection chamber after the silkworm chrysalis for accomplishing fluently kind is collected and cultivates.
Present invention has an advantage that
(1) present invention is cooperated by simple mechanical structure and controller, be can be realized and is accurately and rapidly carried out to silkworm chrysalis
It beats kind, instead of the mode that traditional artificial progress silkworm chrysalis Cordyceps sinensis is beaten kind, it is too low to solve previous silkworm chrysalis Cordyceps sinensis yield, artificial infection mistake
Accidentally more, being inoculated with caused waste not in place to realize the yield of silkworm chrysalis Cordyceps sinensis has great economic value.
(2) the characteristics of present invention beats kind according to silkworm chrysalis Cordyceps sinensis, optimizes automatic injector, send automatic injector to silkworm chrysalis
Direction out tilts 15-30 ° of angle, can be to avoid because of silkworm chrysalis transmission device when the delay of sensor lead to syringe vertical rise and fall
Operating lag and scratch silkworm chrysalis, cause silkworm chrysalis death situation.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples:
Fig. 1 is the structural diagram of the present invention, it is as follows that meaning is respectively identified in figure: 1- automatic injector;2- transmission device;3-
Retarder;4- speed regulating motor;5- silkworm chrysalis transmission device.
Fig. 2 is the structural schematic diagram of control cabinet for power distribution, and it is as follows respectively to identify meaning in figure: (2-1)-cam;(2-2)-connection
Wheel;(2-3)-fixed spring;(2-4)-connecting rod;(2-5)-telescopic rod.
Specific embodiment
In order to which objects and advantages of the present invention are more clearly understood, the present invention is carried out furtherly below in conjunction with example
Bright, the specific embodiments described herein are merely illustrative of the present invention, is not intended to limit the present invention.
Silkworm chrysalis Cordyceps sinensis automatic vaccination equipment, including automatic injector 1, transmission device 2, retarder 3, speed regulating motor 4 and silkworm
Pupa transmission device 5,4 main shaft of speed regulating motor are connect with retarder 3, and 3 output shaft of retarder is connected with transmission device 2, transmission device 2
Automatic injector 1 is installed, silkworm chrysalis transmission device installation 5 is equipped on the lower section of automatic injector 1, silkworm chrysalis transmission device 5
Speed probe installs position sensor on automatic injector 1, and speed probe and position sensor are defeated with controller respectively
Enter end to be connected, controller output end is connected with speed regulating motor 4 and automatic injector 1 respectively.
The transmission device 2 includes cam 2-1, fifth wheel 2-2, fixed spring 2-3, connecting rod 2-4 and telescopic rod 2-
5, the output shaft of retarder 3 is connect with cam 2-1, and cam 2-1 and fifth wheel 2-2 is fixed by fixed spring 2-3, fifth wheel 2-
2 are fixedly connected with connecting rod 24, and connecting rod 2-4 and vertical direction are in 15-30 ° of angle, and connecting rod 2-4 leading portion is equipped with automatic note
Emitter 1.
The conveyor belt design of the silkworm chrysalis transmission device 5 has the groove of 3cm wide.
The automatic injector 1 tilts 20 ° to 5 direction of advance of silkworm chrysalis transmission device.
When silkworm chrysalis transmission device 5 send silkworm chrysalis to 1 lower section of automatic injector, position sensor detects whether to arrive at recess,
When arriving at recess, 2 knit stitch of transmission device, automatic injector 1 injects strain, when position sensor, which detects, arrives at projection, position
Sensor issues signal, and automatic injector 1 rises to original position, and controller control silkworm chrysalis transmission device 5 continues to run, silkworm chrysalis transmission
Speed probe on device 5 monitors revolving speed in real time, and feeds back to controller and regulation is compared, and forms closed-loop control system,
With self-adjustment function, there is incubator in the end of silkworm chrysalis transmission device 5, is sent into after the silkworm chrysalis for accomplishing fluently kind is collected sterile
It is cultivated room.
Silkworm chrysalis Cordyceps sinensis automatic vaccination and breeding method:
(1) silkworm chrysalis sorting and cleaning: rotten, rotten silkworm chrysalis is sub-elected, impurity is removed;
(2) automatic vaccination: the silkworm chrysalis after cleaning is put into silkworm chrysalis transmission device, is connect by silkworm chrysalis Cordyceps sinensis automatic vaccination equipment
Kind, every silkworm chrysalis inoculum concentration control is 3ml;
(3) sterile culture: the silkworm chrysalis for accomplishing fluently kind is cased with 50cmx30cm incubator and is sent into desinfection chamber culture 12 days;
(4) mycelia grows up: cordyceps sinensis cultured in desinfection chamber is put into and continues to cultivate in glass jar, and is fetched water daily 2 times,
Mycelia growth later-stage utilization fluorescent lamp suitably gives illumination and accelerates growth.
(5) cordyceps sinensis harvests: cordyceps sinensis can harvest after cultivating in glass jar 18 days.
Using silkworm chrysalis Cordyceps sinensis automatic vaccination equipment of the present invention, speed is fast, and silkworm chrysalis is not damaged, and the Juncao value grown is high.
Claims (2)
1. silkworm chrysalis Cordyceps sinensis automatic vaccination equipment, it is characterised in that: including automatic injector (1), transmission device (2), retarder
(3), speed regulating motor (4) and silkworm chrysalis transmission device (5), speed regulating motor (4) main shaft are connect with retarder (3), retarder (3) output
Axis is connected with transmission device (2), and transmission device (2) is equipped with automatic injector (1), and silkworm chrysalis transmission device (5) is mounted on certainly
The lower section of syringe (1) is moved, speed probe is installed on silkworm chrysalis transmission device (5), automatic injector is equipped with position on (1)
Sensor, speed probe and position sensor are connected with controller input terminal respectively, and controller output end is electric with speed regulation respectively
Machine (4) is connected with automatic injector (1);
The transmission device (2) includes cam (2-1), fifth wheel (2-2), fixed spring (2-3), connecting rod (2-4) and stretches
The output shaft of contracting bar (2-5), retarder (3) is connect with cam (2-1), and cam (2-1) and fifth wheel (2-2) pass through fixed spring
(2-3) is fixed, and fifth wheel (2-2) is fixedly connected with connecting rod (2-4), and connecting rod (2-4) and vertical direction are in 15-30 ° of angle,
Connecting rod (2-4) leading portion is equipped with automatic injector (1);
The conveyor belt design of the silkworm chrysalis transmission device (5) has the groove of 2-5cm wide;
The automatic injector (1) tilts 15-30 ° to silkworm chrysalis transmission device (5) direction of advance.
2. using silkworm chrysalis Cordyceps sinensis automatic vaccination equipment described in claim 1, it is characterised in that: the mistake of silkworm chrysalis Cordyceps sinensis automatic vaccination
Journey is as follows:
(1) silkworm chrysalis sorting and cleaning: rotten, rotten silkworm chrysalis is sub-elected, impurity is removed;
(2) automatic vaccination: the silkworm chrysalis after cleaning is put into silkworm chrysalis transmission device, is inoculated with by silkworm chrysalis Cordyceps sinensis automatic vaccination equipment,
Every silkworm chrysalis inoculum concentration control is 2-5ml;
(3) sterile culture: the silkworm chrysalis for accomplishing fluently kind is cased with 50cm × 30cm incubator and is sent into desinfection chamber culture 10-15 days;
(4) mycelia grows up: cordyceps sinensis cultured in desinfection chamber is put into and continues to cultivate in glass jar, and is sprayed water 2-3 times daily, bacterium
Silk growth later-stage utilization fluorescent lamp suitably gives illumination and accelerates growth;
(5) cordyceps sinensis harvests: cordyceps sinensis can harvest after cultivating 15-20 days in glass jar.
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CN108384707A (en) * | 2018-05-04 | 2018-08-10 | 兰陵县政和菌业有限公司 | One kind is without taking off the film just inoculable automatic Inoculation machine of Cordyceps militaris strain |
CN108812080A (en) * | 2018-06-26 | 2018-11-16 | 福建神农菇业股份有限公司 | Bottle cultivated edible fungi culture medium connects bacterium channel forming device and channel forming method |
CN112997975B (en) * | 2021-03-01 | 2023-01-20 | 盐城工业职业技术学院 | High-precision automation device suitable for silkworm chrysalis subcutaneous injection |
CN113973652A (en) * | 2021-10-26 | 2022-01-28 | 力源新资源开发(广东)有限公司 | Automatic inoculation equipment for efficiently obtaining cordyceps sinensis |
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CN203087048U (en) * | 2013-01-16 | 2013-07-31 | 湖北欣恺生物科技有限公司 | Edible and medicinal fungus solid fermentation automatic-inoculation machine |
CN204119844U (en) * | 2014-07-26 | 2015-01-28 | 济南展创科技有限公司 | A kind of Cordceps militaris liquid inoculator |
CN104303835A (en) * | 2014-09-29 | 2015-01-28 | 苏州家和农业生物科技研究中心 | Cultivation method of cordyceps sinensis |
CN204482417U (en) * | 2015-02-03 | 2015-07-22 | 湖南和平生物科技有限公司 | A kind of bag-cultivated edible fungi solid spawn automatic vaccination machine |
CN205030184U (en) * | 2015-08-06 | 2016-02-17 | 辽东学院 | Cordycepsmilitaris liquid inoculation machine |
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