CN106190822B - Total plate count detection case in a kind of aquatic products - Google Patents

Total plate count detection case in a kind of aquatic products Download PDF

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Publication number
CN106190822B
CN106190822B CN201610556364.7A CN201610556364A CN106190822B CN 106190822 B CN106190822 B CN 106190822B CN 201610556364 A CN201610556364 A CN 201610556364A CN 106190822 B CN106190822 B CN 106190822B
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Prior art keywords
culture
aquatic products
chamber
dilution chamber
plate
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CN201610556364.7A
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CN106190822A (en
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金雷
张小军
陈瑜
鲍静姣
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Zhejiang Marine Fisheries Research Institute
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Zhejiang Marine Fisheries Research Institute
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses total plate count detection cases in a kind of aquatic products, including incubator ontology, the incubator ontology includes controller, inject dilution chamber and culture sensing chamber, the injection dilution chamber and culture sensing chamber are separated by fixed plate, the injection dilution chamber upper end is equipped with inlet tube and the rubber sleeve of inlet tube upper end, upper end is equipped with fixed link in the injection dilution chamber, the fixed link lower end is equipped with pressing plate, the dead plate upper end is equipped with electric rotating machine, thermoregulator and humidistat, the electric rotating machine is connect by transmission shaft with turntable, the pressing plate is equipped with circular groove, diluted cup is installed between the circular groove and turntable.Total plate count detection case in the aquatic products can maintain the temperature and humidity in detection case in a certain range, to be conducive to the culture and detection of bacterium colony in aquatic products due to the thermoregulator and humidistat in fixed plate.

Description

Total plate count detection case in a kind of aquatic products
Technical field
The present invention relates to testing equipment field, total plate count detection case in specially a kind of aquatic products.
Background technology
The general designation of the animals and plants and its converted products of product ocean and fresh water fishery production, the Micro biological Tests in aquatic products Method is that aquatic product quality manages essential important component, and aquatic products Micro biological Tests are to weigh food hygiene quality One of important indicator, it is the policy for implementing " putting prevention first ", can be effectively prevented or reduce the hair of zoonosis It is raw, ensure the health of the people.For this purpose, we provide total plate count detection case in a kind of aquatic products.
Invention content
The purpose of the present invention is to provide total plate count detection cases in a kind of aquatic products, to solve to carry in above-mentioned background technology The problem of going out.
To achieve the above object, the present invention provides the following technical solutions:Total plate count detection case in a kind of aquatic products, including Incubator ontology, the incubator ontology include controller, injection dilution chamber and culture sensing chamber, the controller outer surface peace Equipped with control switch, operator control panel and display screen, the injection dilution chamber and culture sensing chamber are separated by fixed plate, the fixation Plate is equipped with aperture, and the injection dilution chamber upper end is equipped with inlet tube and the rubber sleeve of inlet tube upper end, the injection dilution Indoor upper end is equipped with fixed link, and the fixed link lower end is equipped with pressing plate, and the dead plate upper end is equipped with electric rotating machine, temperature Adjuster and humidistat, the electric rotating machine are connect by transmission shaft with turntable, and the pressing plate is equipped with circular groove, institute It states and diluted cup is installed between circular groove and turntable.
Preferably, culture detection chamber interior walls both sides are mounted on sliding shoe, and the sliding shoe is equipped with connecting rod, institute It states connecting rod to connect with oscillating rod by rotation axis, the oscillating rod lower end is equipped with detector.
Preferably, the diluted cup lower end is equipped with catheter, and through oscillating rod, the catheter is interior to pacify the catheter Equipped with flowmeter and solenoid valve.
Preferably, the culture sensing chamber bottom end is equipped with culture dish.
Preferably, timer and wireless communication apparatus are installed, the timer and detector are electrical in the controller Connection, the detector are wirelessly connected by wireless communication apparatus and exterior terminal.
Compared with prior art, the beneficial effects of the invention are as follows:Total plate count detection case in the aquatic products, due to fixed plate On thermoregulator and humidistat, can by the temperature and humidity in detection case maintain in a certain range, favorably The culture and detection of bacterium colony in aquatic products can drive turntable to turn due to the diluted cup on turntable by electric rotating machine It is dynamic, in order to the aquatic products sample dilution in diluted cup, due to the flowmeter and solenoid valve in catheter, sample liquid can be controlled The flow on culture dish is flowed into, is cultivated in order to which multiple culture dishes compare, reduces detection error, due to the timing in controller Device can control the detection time of culture dish, can be by the data transmission after detection due to the wireless communication apparatus in controller To exterior terminal, in order to which people take measures.
Description of the drawings
Fig. 1 is overall structure of the present invention;
Fig. 2 is pressure plate structure schematic diagram of the present invention;
Fig. 3 is fixed plate structure schematic diagram of the present invention;
Fig. 4 is controller internal structure schematic diagram of the present invention;
In figure:1 incubator ontology, 2 controllers, 3 culture sensing chamber, 4 humidistats, 5 operator control panels, 6 control switches, 7 Display screen, 8 rotation axis, 9 oscillating rods, 10 detectors, 11 electric rotating machines, 12 transmission shafts, 13 culture dishes, 14 solenoid valves, 15 connections Bar, 16 sliding shoes, 17 thermoregulators, 18 turntables, 19 catheters, 20 pressing plates, 21 fixed links, 22 rubber sleeves, 23 inlet tubes, 24 diluted cups, 25 flowmeters, 26 circular grooves, 27 fixed plates, 28 apertures, 29 timers, 30 wireless communication apparatus, 31 injections are dilute Release room.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
- 4 are please referred to Fig.1, the present invention provides a kind of technical solution:Total plate count detection case in a kind of aquatic products, including training Case ontology 1 is supported, the incubator ontology 1 includes controller 2, injects dilution chamber 31 and culture sensing chamber 3, outside the controller 2 Surface is equipped with control switch 6, operator control panel 5 and display screen 7, and the injection dilution chamber 31 and culture sensing chamber 3 pass through fixed plate 27 separate, and the fixed plate 27 is equipped with aperture 28, and 31 upper end of injection dilution chamber is equipped with inlet tube 23 and inlet tube 23 The rubber sleeve 22 of upper end, upper end is equipped with fixed link 21 in the injection dilution chamber 31, and 21 lower end of the fixed link is equipped with pressing plate 20,27 upper end of the fixed plate is equipped with electric rotating machine 11, thermoregulator 17 and humidistat 4, the electric rotating machine 11 It is connect with turntable 18 by transmission shaft 12, the pressing plate 20 is equipped with circular groove 26, the circular groove 26 and turntable 18 Between diluted cup 24 is installed.
The 3 inner wall both sides of culture sensing chamber are mounted on sliding shoe 16, and the sliding shoe 16 is equipped with connecting rod 15, institute It states connecting rod 15 to connect with oscillating rod 9 by rotation axis 8,9 lower end of the oscillating rod is equipped with detector 10, the diluted cup 24 Lower end is equipped with catheter 19, and the catheter 19 is equipped with flowmeter 25 and electromagnetism through oscillating rod 9 in the catheter 19 Valve 14,3 bottom end of culture sensing chamber are equipped with culture dish 13, timer 29 and wireless telecommunications are equipped in the controller 2 Device 30, the timer 29 are electrically connected with detector 10, and the detector 10 is whole by wireless communication apparatus 30 and outside End is wirelessly connected, can be by temperature in detection case and wet due to the thermoregulator 17 and humidistat 4 in fixed plate 27 Degree maintains in a certain range, and the culture and detection for being conducive to bacterium colony in aquatic products can due to the diluted cup on turntable 18 To drive turntable 18 to rotate by electric rotating machine 11, in order to the aquatic products sample dilution in diluted cup 24, due to catheter Flowmeter 25 in 19 and solenoid valve 14 can control the flow that sample liquid flows on culture dish 13, in order to multiple culture dishes 13 comparison cultures, the detection time of culture dish 13 can be controlled due to the timer 29 in controller 2 by reducing detection error, by In the wireless communication apparatus 30 in controller 2, the data after detection can be transferred to exterior terminal, be arranged in order to which people take It applies.
Operation principle:Total plate count detection case in the aquatic products, the sample of aquatic products is injected by inlet into diluted cup Product, then the physiological saline of a certain amount of isoconcentration gradient is added to diluted cup 24 and is diluted, it is rotated by turntable 18, allows it It is sufficiently mixed, then is flowed into culture dish 13 by catheter 19, after 29 timing of timer, detector 10 after a period of time Colony counts on automatic detection culture dish 13, and the data after detection can be transferred to by outside by wireless communication apparatus 30 Terminal, in order to which people take measures.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace And modification, the scope of the present invention is defined by the appended.

Claims (1)

1. flora sum detection case in a kind of aquatic products, including incubator ontology(1), it is characterised in that:The incubator ontology (1)Including controller(2), injection dilution chamber(31)With culture sensing chamber(3), the controller(2)Outer surface is equipped with control Switch(6), operator control panel(5)And display screen(7), the culture sensing chamber(3)Inner wall both sides are mounted on sliding shoe(16), described Sliding shoe(16)Connecting rod is installed(15), the connecting rod(15)Pass through rotation axis(8)With oscillating rod(9)Connection, the pendulum Lever(9)Lower end is equipped with detector(10), the controller(2)Timer is inside installed(29)And wireless communication apparatus (30), the timer(29)With detector(10)It is electrically connected, the detector(10)Pass through wireless communication apparatus(30)With Exterior terminal is wirelessly connected, the injection dilution chamber(31)With culture sensing chamber(3)Pass through fixed plate(27)Separate, the fixation Plate(27)It is equipped with aperture(28), the injection dilution chamber(31)Upper end is equipped with inlet tube(23)And inlet tube(23)Upper end Rubber sleeve(22), the injection dilution chamber(31)Interior upper end is equipped with fixed link(21), the fixed link(21)Lower end is equipped with pressure Plate(20), the fixed plate(27)Upper end is equipped with electric rotating machine(11), thermoregulator(17)And humidistat(4), institute State electric rotating machine(11)Pass through transmission shaft(12)With turntable(18)Connection, the pressing plate(20)Equipped with circular groove(26), institute State circular groove(26)With turntable(18)Between diluted cup is installed(24), the diluted cup(24)Lower end is equipped with catheter (19), the catheter(19)Through oscillating rod(9), the catheter(19)Flowmeter is inside installed(25)And solenoid valve (14), the culture sensing chamber(3)Bottom end is equipped with culture dish(13).
CN201610556364.7A 2016-07-15 2016-07-15 Total plate count detection case in a kind of aquatic products Active CN106190822B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111272963A (en) * 2020-03-04 2020-06-12 重庆中科检测技术服务有限公司 Hospital wastewater detection device and method
CN112877178B (en) * 2021-03-10 2022-07-29 太原科技大学 High-efficient isolated culture device of actinomycete

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1413889A2 (en) * 2002-10-25 2004-04-28 Sysmex Corporation Sample analyzers, bacteria analyzers, and solutions for diluting and cleaning
CN104266895A (en) * 2014-10-11 2015-01-07 沈佳特 Automatic continuous multi-stage dilution device for bacterial suspension and real-time counting method
CN204714791U (en) * 2015-06-21 2015-10-21 周赞虎 Clinical laboratory's dilution instrument
CN205120476U (en) * 2015-11-20 2016-03-30 刁殿琰 Medical science inspection liquid feeding diluter

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008022835B3 (en) * 2008-05-12 2009-10-22 Torsten Dr. Matthias analyzer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1413889A2 (en) * 2002-10-25 2004-04-28 Sysmex Corporation Sample analyzers, bacteria analyzers, and solutions for diluting and cleaning
CN104266895A (en) * 2014-10-11 2015-01-07 沈佳特 Automatic continuous multi-stage dilution device for bacterial suspension and real-time counting method
CN204714791U (en) * 2015-06-21 2015-10-21 周赞虎 Clinical laboratory's dilution instrument
CN205120476U (en) * 2015-11-20 2016-03-30 刁殿琰 Medical science inspection liquid feeding diluter

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