CN111272963A - Hospital wastewater detection device and method - Google Patents
Hospital wastewater detection device and method Download PDFInfo
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- CN111272963A CN111272963A CN202010141878.2A CN202010141878A CN111272963A CN 111272963 A CN111272963 A CN 111272963A CN 202010141878 A CN202010141878 A CN 202010141878A CN 111272963 A CN111272963 A CN 111272963A
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- 238000001514 detection method Methods 0.000 title claims abstract description 238
- 239000002351 wastewater Substances 0.000 title claims abstract description 120
- 238000000034 method Methods 0.000 title claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 141
- 238000003860 storage Methods 0.000 claims abstract description 87
- 239000000523 sample Substances 0.000 claims abstract description 46
- 238000010438 heat treatment Methods 0.000 claims abstract description 37
- 239000001963 growth medium Substances 0.000 claims abstract description 23
- 238000000926 separation method Methods 0.000 claims abstract description 22
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 241000588724 Escherichia coli Species 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 239000003153 chemical reaction reagent Substances 0.000 claims description 20
- 239000002906 medical waste Substances 0.000 claims description 17
- 239000002609 medium Substances 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims 1
- 230000001678 irradiating effect Effects 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000004090 dissolution Methods 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 239000006152 selective media Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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- G—PHYSICS
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Abstract
The invention discloses a hospital wastewater detection device and method which can detect a plurality of wastewater samples simultaneously, can detect whether escherichia coli exists in wastewater and can ensure detection accuracy. The hospital wastewater detection device comprises a base, a storage battery, a bottom cover, an annular groove, an ultraviolet lamp, a resistance heating device, a central support column, a rotary disc, a liquid separation cylinder, a liquid storage cylinder, a rotary knob, a transparent detection cylinder, a top plate, a coaming, a support rod, a wastewater detection device, a detection probe, a line arrangement pipe, a position indication line, an LED display and a sealing cover. The detection method comprises the following steps: s1, adding a sample, S2, distributing the sample, S3, adding a culture medium, S4 and irradiating ultraviolet rays to detect the concentration of the flora. By adopting the hospital wastewater detection device and method, the detection accuracy can be ensured, the detection steps are simplified, the operation is simple and convenient, and the detection efficiency can be effectively improved.
Description
Technical Field
The invention relates to hospital wastewater treatment, in particular to a hospital wastewater detection device and a hospital wastewater detection method.
Background
It is well known that: the existing hospital waste water generally contains more germs or viruses, and the hospital waste water is a way to cause disease infection.
The existing hospital wastewater is generally discharged after being disinfected and purified. Before hospital waste water is disinfected and purified, hospital waste water needs to be detected, so that harmful substance information and virus and germ information in the hospital waste water are obtained, and a corresponding disinfection and purification process is formulated.
The existing hospital wastewater detection generally adopts a detection device which can only realize the independent detection of one sample, so that the detection accuracy cannot be judged, and the detection result is easy to be wrong when the detection is wrong.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a hospital wastewater detection device which can detect a plurality of wastewater samples at the same time, can detect whether escherichia coli exists in wastewater and can ensure the detection accuracy.
The technical scheme adopted by the invention for solving the technical problems is as follows: the hospital wastewater detection device comprises a base; the upper surface of the base is provided with an annular groove;
the annular groove is internally provided with ultraviolet lamps which are uniformly distributed along the circumference and resistance heaters which are uniformly distributed along the circumference; the resistance heating devices are distributed at intervals; the outer circumferential surface of the base is provided with a control button for controlling the ultraviolet lamp and the resistance heating device;
a storage battery for providing electric energy for the ultraviolet lamp and the resistance heating device is arranged in the base; a central support column is arranged on the base;
a turntable is rotatably arranged above the central support column; the turntable is provided with limiting holes uniformly distributed along the circumference; a transparent detection cylinder is arranged in the limiting hole; the lower end of the transparent detection cylinder is positioned in the annular groove; a liquid separation cylinder is arranged above the rotary table; the lower end of the liquid separation cylinder is fixedly connected with the central support column;
liquid distributing pipes which are uniformly distributed along the circumference are arranged on the outer circumferential surface of the liquid distributing cylinder; the liquid separating pipe is communicated with the inner cavity of the liquid separating cylinder; the liquid distribution pipe extends to the upper part of the transparent detection cylinder;
a liquid storage cylinder is rotatably arranged in the liquid separation cylinder; the lower end of the liquid storage cylinder is provided with a liquid leakage hole; the liquid outlet cylinder rotates to enable the liquid leakage holes to be aligned with the liquid distribution pipe; the upper end of the liquid storage cylinder is provided with a knob for rotating the liquid storage cylinder; the upper end of the liquid storage cylinder is provided with a sealing cover;
an annular supporting plate is arranged above the turntable; a support rod is arranged between the annular support plate and the turntable;
a waste water detection device is arranged on the annular supporting plate; the waste water detection device is provided with a detection probe, and the detection probe penetrates through the annular supporting plate and extends into the transparent detection cylinder;
the annular supporting plate is provided with a position marking line of the transparent detection cylinder; an alignment line for marking the position of the liquid leakage hole is arranged on the knob;
the wastewater detection device comprises a detection processor and a detection reagent storage box; the detection probe comprises a liquid outlet pipe communicated with the detection reagent storage box and a detection sensor arranged at the lower end of the liquid outlet pipe; a valve is arranged on the liquid outlet pipe; the detection sensor is electrically connected with the detection processor; and an LED display is arranged on the wastewater detection device.
Furthermore, a surrounding plate is arranged on the supporting plate, the LED display screen is arranged on the surrounding plate, and the wastewater detection device is arranged in the surrounding plate.
Furthermore, a mounting groove with an opening at the bottom is arranged in the base, the storage battery is mounted in the mounting groove, and a bottom cover for sealing the mounting groove is arranged at the bottom of the base.
Furthermore, a valve on the liquid outlet pipe adopts an electromagnetic valve.
The invention also provides a method for detecting the hospital wastewater by adopting the hospital wastewater detection device, which comprises the following steps:
s1, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder, and screwing the knob to seal a liquid leakage hole in the liquid storage cylinder by the liquid separation cylinder; the waste water is stored in the liquid storage cylinder;
s2, the liquid storage cylinders respectively flow into the transparent detection cylinders by screwing the knob, so that the quantity of each transparent detection cylinder is the same;
s3, injecting the culture medium stored in the detection reagent storage box into each transparent detection cylinder through a liquid outlet pipe on the wastewater detection device; injecting corresponding culture media according to the floras to be detected in each transparent detection cylinder;
s4, rotating the turntable to enable the transparent detection cylinder to be positioned above the ultraviolet lamp, and then turning on the ultraviolet lamp to irradiate the transparent detection cylinder; the irradiation time is 30min to 60 min;
then, a signal detected by a detection sensor arranged at the lower end of the liquid outlet pipe is transmitted to a wastewater detection device, and the concentration of the flora to be detected in the medical wastewater to be detected is detected by the wastewater detection device.
Furthermore, the invention also discloses a method for detecting escherichia coli in hospital wastewater by using the hospital wastewater detection device, which comprises the following steps:
b1, storing the prepared selective culture medium in a detection reagent storage box of the wastewater detection device, wherein 7.5g of the selective culture medium is weighed, and 10mL of deionized water preheated to 42 +/-1 ℃ is added for full dissolution;
b2, setting a prepared enrichment medium in the transparent detection cylinder; 26.5g of enrichment medium is weighed and added with 1L of deionized water preheated to 42 +/-1 ℃ for fully dissolving;
b3, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder, and screwing the knob at the moment to seal a liquid leakage hole in the liquid storage cylinder by the liquid separation cylinder; the waste water is stored in the liquid storage cylinder; the liquid storage cylinders respectively flow into the transparent detection cylinders to be mixed with the enrichment medium by screwing the knob;
b4, rotating the turntable to enable the transparent detection cylinder to be located above the heating device, and simultaneously controlling the heating device to control the temperature in the transparent detection cylinder to be 42 +/-1 ℃; and statically culturing for 6h under the temperature environment;
b5, and opening a valve on the liquid outlet pipe to enable the selective culture medium stored in the detection reagent storage box to be injected into the transparent detection cylinder; controlling the heating device, controlling the temperature in the transparent detection cylinder to be 42 +/-1 ℃, and continuously culturing for 18-24 h;
b6, controlling a heating device, heating the mixed solution to boiling, heating for 10min, and cooling to room temperature;
and B7, taking the solution in the transparent detection cylinder, dripping 3-4 drops of the solution into a sample hole of the colloidal gold test paper by using a dropper, reacting for 5-10min, and judging the result, wherein the color development is ineffective after more than 30 min.
The invention has the beneficial effects that: the hospital wastewater detection device provided by the invention is simple in structure, is convenient for realizing detection on multiple groups of detection samples at the same time, and ensures that the detection conditions of each sample are the same, so that comparison detection can be carried out, and the detection accuracy can be ensured. Secondly, the hospital wastewater detection device can realize irradiation and heating of ultraviolet rays, and then detect the sample, so that the detection accuracy can be improved, and secondly, the color change of the detected sample can be observed by naked eyes conveniently.
The hospital wastewater detection method provided by the invention can simplify detection steps, is simple and convenient to operate, and can effectively improve detection efficiency.
Drawings
FIG. 1 is an exploded view of a hospital waste water detection device according to an embodiment of the present invention;
FIG. 2 is a perspective view of a hospital waste water test apparatus according to an embodiment of the present invention;
FIG. 3 is a front view of a hospital waste water test apparatus according to an embodiment of the present invention;
FIG. 4 is a top view of a hospital waste water test device in an embodiment of the present invention;
FIG. 5 is a cross-sectional view B-B of FIG. 4;
FIG. 6 is a schematic structural diagram of a wastewater detection apparatus according to an embodiment of the present invention;
the following are marked in the figure: 1-base, 2-accumulator, 3-bottom cover, 4-ring groove, 5-ultraviolet lamp, 6-resistance heating device, 7-central support column, 8-rotary table, 9-liquid separating cylinder, 10-liquid storage cylinder, 11-knob, 12-transparent detection cylinder, 13-top plate, 14-coaming plate, 15-support rod, 16-waste water detection device, 17-detection probe, 172-line arrangement pipe, 18-position marking line, 19-LED display, 20-sealing cover.
Detailed Description
The invention is further illustrated with reference to the following figures and examples.
As shown in fig. 1-4, the hospital wastewater testing apparatus of the present invention comprises a base 1; the upper surface of the base 1 is provided with an annular groove 4;
the annular groove 4 is internally provided with ultraviolet lamps 5 which are uniformly distributed along the circumference and resistance heaters 6 which are uniformly distributed along the circumference; the resistance heating devices 6 are distributed at intervals; the outer circumferential surface of the base 1 is provided with a control button for controlling the ultraviolet lamp 5 and the resistance heating device 6;
a storage battery 2 for supplying electric energy to the ultraviolet lamp 5 and the resistance heating device 6 is arranged in the base 1; a central support column 7 is arranged on the base 1;
a turntable 8 is rotatably arranged above the central support column 7; the turntable 8 is provided with limiting holes 81 uniformly distributed along the circumference; the transparent detection cylinder 12 is arranged in the limiting hole 81; the lower end of the transparent detection cylinder 12 is positioned in the annular groove 4; a liquid separation cylinder 9 is arranged above the rotary table 8; the lower end of the liquid separation cylinder 9 is fixedly connected with the central support column 7;
the outer circumferential surface of the liquid separation cylinder 9 is provided with liquid separation pipes 91 which are uniformly distributed along the circumference; the liquid separating pipe 91 is communicated with the inner cavity of the liquid separating cylinder 9; the liquid separation tube 91 extends to the upper part of the transparent detection cylinder 12;
a liquid storage cylinder 10 is rotatably mounted in the liquid separation cylinder 9; the lower end of the liquid storage cylinder 10 is provided with a liquid leakage hole 101; the liquid outlet cylinder 10 rotates to enable the liquid leakage holes 101 to be aligned with the liquid dividing pipe 91; the upper end of the liquid storage cylinder 10 is provided with a knob 11 for rotating the liquid storage cylinder 10; the upper end of the liquid storage cylinder 10 is provided with a sealing cover 20;
an annular supporting plate 13 is arranged above the rotary table 8; a support rod 15 is arranged between the annular support plate 13 and the rotary table 8;
the annular supporting plate 13 is provided with a waste water detection device 16; the waste water detection device 16 is provided with a detection probe 17, and the detection probe 17 extends into the transparent detection cylinder 12 through the annular supporting plate 13;
the annular supporting plate 13 is provided with a position marking line 18 of the transparent detection cylinder 12; an alignment line for marking the position of the liquid leakage hole 101 is arranged on the knob 11;
the wastewater detection device 16 includes a detection processor 162 and a detection reagent storage tank 161; the detection probe 17 comprises a liquid outlet pipe 171 communicated with the detection reagent storage box 161 and a detection sensor 173 arranged at the lower end of the liquid outlet pipe 171; a valve is arranged on the liquid outlet pipe 171; the detection sensor 173 is electrically connected with the detection processor 162; an LED display 19 is arranged on the wastewater detection device 16.
During the application process, the sample in the liquid storage cylinder 10 is distributed to the transparent detection cylinder 12 through the liquid separation cylinder 9, and the transparent detection cylinder 12 is irradiated by ultraviolet rays and heated by rotating the rotary disc 8. And the detection of the sample in the transparent detection cylinder 12 can be realized by the detection probe 17 of the waste water detection device 16.
In conclusion, the hospital wastewater detection device provided by the invention is simple in structure, is convenient for simultaneously detecting multiple groups of detection samples, can detect whether escherichia coli exists in wastewater, and ensures that the detection conditions of all samples are the same, so that comparison detection can be carried out, and the detection accuracy can be ensured. Secondly, the hospital wastewater detection device can realize irradiation and heating of ultraviolet rays, and then detect the sample, so that the detection accuracy can be improved, and secondly, the color change of the detected sample can be observed by naked eyes conveniently.
In order to facilitate the comparison and observation of the detection result, the device structure is simplified, and further, a surrounding plate 14 is arranged on the supporting plate 13, the LED display screen 19 is arranged on the surrounding plate 14, and the wastewater detection device 16 is arranged in the surrounding plate 14.
In order to facilitate the replacement of the storage battery, a mounting groove with an opening at the bottom is arranged in the base 1, the storage battery 2 is arranged in the mounting groove, and a bottom cover 3 for sealing the mounting groove is arranged at the bottom of the base 1.
For convenience of control, it is preferable that the valve on the outlet pipe 171 is a solenoid valve.
The invention also provides a method for detecting the hospital wastewater by adopting the hospital wastewater detection device, which comprises the following steps:
s1, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder 10, and screwing the knob 11 to seal the liquid leakage hole 101 in the liquid storage cylinder 10 by the liquid separation cylinder 9; the waste water is stored in the liquid storage cylinder 10;
s2, the liquid storage cylinders 10 respectively flow into the transparent detection cylinders 12 by screwing the knob 11, so that the amount of each transparent detection cylinder 12 is the same;
s3, injecting the culture medium stored in the reagent storage box 161 into each transparent cartridge 12 through the outlet tube 171 of the waste water detecting device 16; injecting corresponding culture medium according to the flora to be detected in each transparent detection cylinder 12;
s4, rotating the turntable to enable the transparent detection cylinder to be positioned above the ultraviolet lamp, and then turning on the ultraviolet lamp to irradiate the transparent detection cylinder; the irradiation time is 30min to 60 min;
then, a signal detected by a detection sensor 173 provided at the lower end of the liquid outlet pipe 171 is transmitted to the wastewater detection device 16, and the concentration of the bacterial flora to be detected in the medical wastewater to be detected is detected by the wastewater detection device.
The specific steps of detecting the concentration of the flora by using the wastewater detection device 16 are disclosed in patent application No. 201110461631.X, and the detection device and the detection method for medical wastewater are disclosed in detail.
Furthermore, the invention also discloses a method for detecting escherichia coli in hospital wastewater by using the hospital wastewater detection device, which comprises the following steps:
b1, storing the prepared selective culture medium in a detection reagent storage box 161 of the wastewater detection device 16, wherein 7.5g of the selective culture medium is weighed, and 10mL of deionized water preheated to 42 +/-1 ℃ is added for full dissolution;
b2, setting a prepared enrichment medium in the transparent detection cylinder 12; 26.5g of enrichment medium is weighed and added with 1L of deionized water preheated to 42 +/-1 ℃ for fully dissolving;
b3, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder 10, and screwing the knob 11 to seal the liquid leakage hole 101 in the liquid storage cylinder 10 by the liquid separation cylinder 9; the waste water is stored in the liquid storage cylinder 10; the liquid storage cylinders 10 respectively flow into the transparent detection cylinder 12 to be mixed with the enrichment medium by screwing the knob 11;
b4, rotating the turntable to enable the transparent detection cylinder to be positioned above the heating device, and simultaneously controlling the heating device to control the temperature in the transparent detection cylinder 12 to be 42 +/-1 ℃; and statically culturing for 6h under the temperature environment;
b5 and opening the valve in the outlet tube 171 to allow the selective medium stored in the testing reagent storage cassette 161 to be injected into the transparent cartridge 12; controlling the heating device, controlling the temperature in the transparent detection cylinder 12 to be 42 +/-1 ℃ and continuing culturing for 18-24 h;
b6, controlling a heating device, heating the mixed solution to boiling, heating for 10min, and cooling to room temperature;
and B7, taking the solution in the transparent detection cylinder 12, dripping 3-4 drops of the solution into a sample hole of the colloidal gold test paper by using a dropper, reacting for 5-10min, and judging the result, wherein the color development is ineffective after 30 min.
Example one
By adopting the hospital wastewater detection device, 100ml of hospital wastewater samples are taken, and the concentration of the flora in the wastewater is detected according to the following steps:
s1, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder 10, and screwing the knob 11 to seal the liquid leakage hole 101 in the liquid storage cylinder 10 by the liquid separation cylinder 9; the waste water is stored in the liquid storage cylinder 10;
s2, the liquid storage cylinders 10 respectively flow into the transparent detection cylinders 12 by screwing the knob 11, so that the amount of each transparent detection cylinder 12 is the same;
s3, injecting the culture medium stored in the reagent storage box 161 into each transparent cartridge 12 through the outlet tube 171 of the waste water detecting device 16; injecting corresponding culture medium according to the flora to be detected in each transparent detection cylinder 12;
s4, rotating the turntable to enable the transparent detection cylinder to be positioned above the ultraviolet lamp, and then turning on the ultraviolet lamp to irradiate the transparent detection cylinder; the irradiation time is 30 min;
then, a signal detected by a detection sensor 173 provided at the lower end of the liquid outlet pipe 171 is transmitted to the wastewater detection device 16, and the concentration of the bacterial flora to be detected in the medical wastewater to be detected is detected by the wastewater detection device.
Example two
By adopting the hospital wastewater detection device, 100ml of hospital wastewater samples are taken, and the concentration of the flora in the wastewater is detected according to the following steps:
s1, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder 10, and screwing the knob 11 to seal the liquid leakage hole 101 in the liquid storage cylinder 10 by the liquid separation cylinder 9; the waste water is stored in the liquid storage cylinder 10;
s2, the liquid storage cylinders 10 respectively flow into the transparent detection cylinders 12 by screwing the knob 11, so that the amount of each transparent detection cylinder 12 is the same;
s3, injecting the culture medium stored in the reagent storage box 161 into each transparent cartridge 12 through the outlet tube 171 of the waste water detecting device 16; injecting corresponding culture medium according to the flora to be detected in each transparent detection cylinder 12;
s4, rotating the turntable to enable the transparent detection cylinder to be positioned above the ultraviolet lamp, and then turning on the ultraviolet lamp to irradiate the transparent detection cylinder; the irradiation time is 45 min;
then, a signal detected by a detection sensor 173 provided at the lower end of the liquid outlet pipe 171 is transmitted to the wastewater detection device 16, and the concentration of the bacterial flora to be detected in the medical wastewater to be detected is detected by the wastewater detection device.
EXAMPLE III
By adopting the hospital wastewater detection device, 100ml of hospital wastewater samples are taken, and the concentration of the flora in the wastewater is detected according to the following steps:
s1, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder 10, and screwing the knob 11 to seal the liquid leakage hole 101 in the liquid storage cylinder 10 by the liquid separation cylinder 9; the waste water is stored in the liquid storage cylinder 10;
s2, the liquid storage cylinders 10 respectively flow into the transparent detection cylinders 12 by screwing the knob 11, so that the amount of each transparent detection cylinder 12 is the same;
s3, injecting the culture medium stored in the reagent storage box 161 into each transparent cartridge 12 through the outlet tube 171 of the waste water detecting device 16; injecting corresponding culture medium according to the flora to be detected in each transparent detection cylinder 12;
s4, rotating the turntable to enable the transparent detection cylinder to be positioned above the ultraviolet lamp, and then turning on the ultraviolet lamp to irradiate the transparent detection cylinder; the irradiation time is 60 min;
then, a signal detected by a detection sensor 173 provided at the lower end of the liquid outlet pipe 171 is transmitted to the wastewater detection device 16, and the concentration of the bacterial flora to be detected in the medical wastewater to be detected is detected by the wastewater detection device.
Example four
By adopting the hospital wastewater detection device, 100ml of hospital wastewater samples are taken, and escherichia coli is detected according to the following steps:
b1, storing the prepared selective culture medium in a detection reagent storage box 161 of the wastewater detection device 16, wherein 7.5g of the selective culture medium is weighed, and 10mL of deionized water preheated to 42 +/-1 ℃ is added for full dissolution;
b2, setting a prepared enrichment medium in the transparent detection cylinder 12; 26.5g of enrichment medium is weighed and added with 1L of deionized water preheated to 42 +/-1 ℃ for fully dissolving;
b3, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder 10, and screwing the knob 11 to seal the liquid leakage hole 101 in the liquid storage cylinder 10 by the liquid separation cylinder 9; the waste water is stored in the liquid storage cylinder 10; the liquid storage cylinders 10 respectively flow into the transparent detection cylinder 12 to be mixed with the enrichment medium by screwing the knob 11;
b4, rotating the turntable to enable the transparent detection cylinder to be positioned above the heating device, and simultaneously controlling the heating device to control the temperature in the transparent detection cylinder 12 to be 42 +/-1 ℃; and statically culturing for 6h under the temperature environment;
b5 and opening the valve in the outlet tube 171 to allow the selective medium stored in the testing reagent storage cassette 161 to be injected into the transparent cartridge 12; controlling the heating device, controlling the temperature in the transparent detection cylinder 12 to be 42 +/-1 ℃ and continuing culturing for 18-24 h;
b6, controlling a heating device, heating the mixed solution to boiling, heating for 10min, and cooling to room temperature;
and B7, taking the solution in the transparent detection cylinder 12, dripping 3-4 drops of the solution into a sample hole of the colloidal gold test paper by using a dropper, reacting for 5-10min, and judging the result, wherein the color development is ineffective after 30 min.
Claims (6)
1. Hospital's waste water detection device, its characterized in that: comprises a base (1); an annular groove (4) is formed in the upper surface of the base (1);
ultraviolet lamps (5) which are uniformly distributed along the circumference and resistance heaters (6) which are uniformly distributed along the circumference are arranged in the annular groove (4); the resistance heating devices (6) are distributed at intervals; the outer circumferential surface of the base (1) is provided with a control button for controlling the ultraviolet lamp (5) and the resistance heating device (6);
a storage battery (2) for providing electric energy for the ultraviolet lamp (5) and the resistance heating device (6) is arranged in the base (1); a central support column (7) is arranged on the base (1);
a turntable (8) is rotatably arranged above the central supporting column (7); limiting holes (81) are uniformly distributed along the circumference on the rotary table (8); a transparent detection cylinder (12) is arranged in the limiting hole (81); the lower end of the transparent detection cylinder (12) is positioned in the annular groove (4); a liquid separation cylinder (9) is arranged above the rotary disc (8); the lower end of the liquid separation cylinder (9) is fixedly connected with the central support column (7);
liquid distributing pipes (91) which are uniformly distributed along the circumference are arranged on the outer circumferential surface of the liquid distributing cylinder (9); the liquid separating pipe (91) is communicated with the inner cavity of the liquid separating cylinder (9); the liquid separating pipe (91) extends to the upper part of the transparent detection cylinder (12);
a liquid storage cylinder (10) is rotatably mounted in the liquid separation cylinder (9); the lower end of the liquid storage cylinder (10) is provided with a liquid leakage hole (101); the liquid outlet cylinder (10) rotates to enable the liquid leakage holes (101) to be aligned with the liquid distribution pipe (91); the upper end of the liquid storage cylinder (10) is provided with a knob (11) for rotating the liquid storage cylinder (10); the upper end of the liquid storage cylinder (10) is provided with a sealing cover (20);
an annular supporting plate (13) is arranged above the rotary table (8); a support rod (15) is arranged between the annular support plate (13) and the turntable (8);
a waste water detection device (16) is arranged on the annular supporting plate (13); the waste water detection device (16) is provided with a detection probe (17), and the detection probe (17) penetrates through the annular supporting plate (13) and extends into the transparent detection cylinder (12);
the annular supporting plate (13) is provided with a position marking line (18) of the transparent detection cylinder (12); an alignment line for marking the position of the liquid leakage hole (101) is arranged on the knob (11);
the wastewater detection device (16) comprises a detection processor (162) and a detection reagent storage box (161); the detection probe (17) comprises a liquid outlet pipe (171) communicated with the detection reagent storage box (161) and a detection sensor (173) arranged at the lower end of the liquid outlet pipe (171); a valve is arranged on the liquid outlet pipe (171); the detection sensor (173) is electrically connected with the detection processor (162); and an LED display (19) is arranged on the wastewater detection device (16).
2. The hospital waste water detection device according to claim 1, characterized in that: be provided with bounding wall (14) on backup pad (13), LED display screen (19) set up on bounding wall (14), waste water detection device (16) sets up in bounding wall (14).
3. The hospital waste water detection device according to claim 2, characterized in that: the storage battery charging device is characterized in that a mounting groove with an opening at the bottom is formed in the base (1), the storage battery (2) is mounted in the mounting groove, and a bottom cover (3) for sealing the mounting groove is arranged at the bottom of the base (1).
4. The hospital waste water detection device according to claim 3, characterized in that: and a valve on the liquid outlet pipe (171) adopts an electromagnetic valve.
5. The method for detecting hospital waste water by using the hospital waste water detection device according to any one of claims 1 to 4, comprising the steps of:
s1, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder (10), and screwing the knob (11) to seal the liquid leakage hole (101) in the liquid storage cylinder (10) by the liquid distribution cylinder (9); the waste water is stored in the liquid storage cylinder (10);
s2, the liquid storage cylinders (10) respectively flow into the transparent detection cylinders (12) by screwing the knob (11), so that the amount of each transparent detection cylinder (12) is the same;
s3, injecting the culture medium stored in the detection reagent storage box (161) into each transparent detection cylinder (12) through a liquid outlet pipe (171) on the waste water detection device (16); injecting corresponding culture media according to the flora to be detected in each transparent detection cylinder (12);
s4, rotating the turntable to enable the transparent detection cylinder to be positioned above the ultraviolet lamp, and then turning on the ultraviolet lamp to irradiate the transparent detection cylinder; the irradiation time is 30min to 60 min;
then, a signal detected by a detection sensor (173) arranged at the lower end of the liquid outlet pipe (171) is transmitted to a waste water detection device (16), and the concentration of the flora to be detected in the medical waste water to be detected is detected by the waste water detection device.
6. The method for detecting Escherichia coli in hospital waste water by using the hospital waste water detection device according to any one of claims 1 to 4, comprising the steps of:
b1, storing the prepared selective culture medium in a detection reagent storage box (161) of the wastewater detection device (16), weighing 7.5g of the selective culture medium, adding 10mL of deionized water preheated to 42 +/-1 ℃, and fully dissolving;
b2, setting a prepared enrichment medium in the transparent detection cylinder (12); 26.5g of enrichment medium is weighed and added with 1L of deionized water preheated to 42 +/-1 ℃ for fully dissolving;
b3, taking a hospital wastewater detection sample, putting the hospital wastewater detection sample into the liquid storage cylinder (10), and screwing the knob (11) to seal a liquid leakage hole (101) on the liquid storage cylinder (10) by the liquid distribution cylinder (9); the waste water is stored in the liquid storage cylinder (10); the liquid storage cylinders (10) respectively flow into the transparent detection cylinder (12) to be mixed with the enrichment medium by screwing the knob (11);
b4, rotating the turntable to enable the transparent detection cylinder to be positioned above the heating device, and simultaneously controlling the heating device to control the temperature in the transparent detection cylinder (12) to be 42 +/-1 ℃; and statically culturing for 6h under the temperature environment;
b5, and opening a valve on the liquid outlet pipe (171) to allow the selective culture medium stored in the detection reagent storage box (161) to be injected into the transparent detection cylinder (12); controlling the heating device, controlling the temperature in the transparent detection cylinder (12) to be 42 +/-1 ℃ and continuing culturing for 18-24 h;
b6, controlling a heating device, heating the mixed solution to boiling, heating for 10min, and cooling to room temperature;
and B7, taking the solution in the transparent detection cylinder (12), dripping 3-4 drops of the solution to a sample hole of the colloidal gold test paper by using a dropper, reacting for 5-10min, and judging the result that the color development is ineffective after more than 30 min.
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Application publication date: 20200612 |