CN108318466A - A kind of food safety detection method - Google Patents
A kind of food safety detection method Download PDFInfo
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- CN108318466A CN108318466A CN201810322386.6A CN201810322386A CN108318466A CN 108318466 A CN108318466 A CN 108318466A CN 201810322386 A CN201810322386 A CN 201810322386A CN 108318466 A CN108318466 A CN 108318466A
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- plate
- insulating box
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- 238000001514 detection method Methods 0.000 title claims abstract description 89
- 230000007246 mechanism Effects 0.000 claims abstract description 80
- 238000004806 packaging method and process Methods 0.000 claims abstract description 53
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000007853 buffer solution Substances 0.000 claims abstract description 14
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000284 extract Substances 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 11
- 230000004888 barrier function Effects 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
- 230000013011 mating Effects 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000011435 rock Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 12
- 238000010586 diagram Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to field of food detection, specifically a kind of food safety detection method, this method is detected using detection device, detection device extracts sample by Qu Ye mechanisms, it is manually controlled by touch screen, packaging is carried out to the sample that needs detect by luminous tube, it is manipulated by control box, the addition of buffer solution and fluorescein mixed liquor is carried out by charging mechanism, suitable environment is improved by insulating box, luminous tube is gripped by clamping device, the progress of charging mechanism is controlled by control mechanism, it is detected by testing agency；It is separated with the position of detection using will be operated with insulating box by control box, while independent enclosure space can effectively avoid the interference of other miscellaneous bacterias.This method is hand-held without operator, can not only be carried out at the same time multiple detections, and the accuracy of detection is ensured by control, reduces operation labour, improves precision.
Description
Technical field
The present invention relates to field of food detection, specifically a kind of food safety detection method.
Background technology
The detection of food bacteria containing amount be judge its whether the important means of safety, have an important role, it is existing to contain bacterium
The detection mode of amount mostly uses greatly the microbial contamination in microbial atp biloluminescence method measurement food, and principle is to utilize
Biochemical action makes the ATP of bacterial cell shine, and measures amount of bacteria with its brightness, which detects rapid sensitive, use model
It encloses extensively.
Existing detector is artificial before detection to add buffer solution and fluorescein mixture directly under exposed environment, this
Kind mode is easily mixed into miscellaneous bacteria, while generally can only once detect a luminous tube, and there are prodigious contingency.In consideration of it, this hair
It is bright to provide a kind of food safety detection method, it has the characteristics that：
(1) detection device that this method uses, the position that will be operated with detect using the control box and the insulating box
It is separated, while independent enclosure space can effectively avoid the interference of other miscellaneous bacterias.
(2) detection device that this method uses, before detection, using three luminous tube packaging samples, using described
Clamping device is clamped fixation, hand-held without operator, can not only be carried out at the same time multiple detections, ensures detection by control
Accuracy reduces labour when operation.
(3) detection device that this method uses can be directly by it when carrying out the addition of buffer solution and fluorescein mixed liquor
It is placed in the inside in the packaging hole, the two is made in the way of extruding while entering the inside of multiple luminous tubes, significantly
Labour is reduced, the probability of error is reduced, improves the precision of detection.
(4) detection device that this method uses makes when entering the inside of the detection babinet using the motor
It is whole reciprocal shaking occur, it is ensured that internal mix is uniform, while before entry to the luminous tube carry out capping convenient for its into
Enter to the detection babinet and is used for quickly detecting.
Invention content
For the problems of the prior art, the present invention provides the inspections that a kind of food safety detection method, this method use
Measurement equipment extracts sample by Qu Ye mechanisms, is manually controlled by touch screen, the sample detected to needs by luminous tube
Packaging is carried out, is manipulated by control box, the addition of buffer solution and fluorescein mixed liquor is carried out by charging mechanism, passes through perseverance
Incubator improves suitable environment, is gripped to luminous tube by clamping device, charging mechanism is controlled by control mechanism
Progress, be detected by testing agency；It is separated with the position of detection using will be operated with insulating box by control box,
Independent enclosure space can effectively avoid the interference of other miscellaneous bacterias simultaneously；Before detection, three luminous tube packaging samples, profit are utilized
It is clamped fixation with clamping device, it is hand-held without operator, multiple detections can be not only carried out at the same time, ensure to detect by control
Accuracy, reduce operation when labour；When carrying out the addition of buffer solution and fluorescein mixed liquor, can directly place it in
The inside in packaging hole is made the two in the way of extruding while entering the inside of multiple luminous tubes, substantially reduced labour, subtract
The probability to malfunction less improves the precision of detection；When entering the inside of detection babinet, keep whole appearance reciprocal using motor
Shaking, it is ensured that internal mix is uniform, at the same before entry to luminous tube carry out capping convenient for its enter detection babinet progress
Quickly detection.
The technical solution adopted by the present invention to solve the technical problems is：A kind of food safety detection method, this method are adopted
It is detected with detection device, the detection device includes Qu Ye mechanisms, touch screen, luminous tube, control box, charging mechanism, perseverance
Incubator, clamping device, control mechanism and testing agency；The control box is internally provided with for taking liquid described in packaging and liquid separation
Mechanism, the luminous tube of the Qu Ye mechanisms mating connection liquid separation packaging；Being internally provided with for the control box adds for auxiliary material
The charging mechanism added, the charging mechanism mating connection luminous tube, and controlled described in the bottom of the charging mechanism
Mechanism；The insulating box is fixed in the bottom of the control box, and the insulating box is internally provided with clamping and mobile described shines
The clamping device of pipe；The insulating box is internally provided with the testing agency, the Qu Ye mechanisms, the charging mechanism,
The insulating box, the clamping device, the control mechanism and the testing agency are electrically connected the touch screen；
Detection method includes the following steps for this：
Step 1, Qu Ye mechanisms extract detection sample；
The sample of extraction is sent into multiple luminous tubes by step 2, the Qu Ye mechanisms in step 1；
Luminous tube in step 2 is moved at charging mechanism position by step 3, clamping device, charging mechanism to shine
Buffer solution and fluorescein are added in pipe；Then, clamping device rocks luminous tube, so that the solution in luminous tube is mixed equal
It is even；
Step 4, after step 3, luminous tube is transported in testing agency and is detected by clamping device.
Specifically, the Qu Ye mechanisms include liquid-collecting bottle, fixed ring, solenoid valve and first time material head, the fixed ring is solid
Due to the side-walls of the control box, and the fixed ring is connected together the liquid-collecting bottle；The bottom of the liquid-collecting bottle is connected to institute
Solenoid valve is stated, and the solenoid valve is electrically connected the touch screen；The bottom of the solenoid valve connects described first by hose
Lower material head；First time material head is equipped with three, and the quantity of first time material head, spacing and the luminous tube spacing, number
It measures identical.
Specifically, the surface of the control box opens up the handle hole of symmetrical two circular hole structures, and the control box
Bottom weld the insulating box；The side of the insulating box is equipped with transparency window, and the transparency window is rotatablely connected the constant temperature
Case；Handle is fixed in the side of the transparency window.
Specifically, the charging mechanism include pull rod, connecting rod, second time material head, cover board, connecting plate, piston, packaging hole and
Packaging case；The packaging case is fixed on the top of the control box, and the inside of the packaging case opens up two rows of packaging holes；
The bottom in the packaging hole is connected to second time material head, and second time material head runs through the packaging case；The collection
The internal slide for filling hole connects the piston, and the connecting rod is fixed at the top of the piston；Institute is fixed at the top of the connecting rod
Connecting plate is stated, and the pull rod is fixed at the top of the connecting plate；The pull rod is slidably connected the cover board, and the cover board card
It closes and connects the packaging case.
Specifically, the quantity in the single packaging hole, spacing are identical as quantity, the spacing of first time material head, and
The height in the packaging hole, the height of the connecting rod and bottom are that opening, the height of the cover board of hollow-core construction are identical.
Specifically, the clamping device includes motor, the first electric pushrod, connecting rod and retaining paw, the motor insertion
To the inside of the insulating box, and one end of the motor connects first electric pushrod；The one of first electric pushrod
The fixed connecting rod in end, and three retaining paws are fixed in the side of the connecting rod.
Specifically, the quantity of the retaining paw, spacing and quantity, the spacing of first time material head are identical and described
Retaining paw includes clamping plate, limiting plate, the second electric pushrod and plate tooth；The limiting plate is fixed on the side of the connecting rod, and
Second electric pushrod is fixed in the inside of the limiting plate；The side of second electric pushrod connects the clamping plate, and institute
It states clamping plate and is rotatablely connected the limiting plate；The inside of the clamping plate is equipped with the plate tooth；The clamping plate uses semicircular structure,
And the inside of the clamping plate is equidistantly equipped with the plate tooth of several rubber structures.
Specifically, the control mechanism is set to the bottom end of the charging mechanism, the control mechanism includes by compressing switch, live
Movable plate and the first torsional spring, the movable plate are connected to the inside of the insulating box, and the movable plate by first torsional spring
Side be equipped with it is described by compressing switch；It is described to be electrically connected first electric pushrod and the touch screen by compressing switch.
Specifically, the testing agency includes light barrier, detection babinet, feed inlet, the second torsional spring and baffle, the detection
Babinet is fixed on the inside of the insulating box, and the side of the detection babinet opens up the feed inlet；The top of the feed inlet
The baffle is fixed in portion, and the side of the feed inlet connects the light barrier by second torsional spring.
Specifically, the baffle uses the top that the feed inlet is fixed in inclination angle for 30 ° -45 °, and the bottom of the baffle
Portion is equipped with several protrusions；The side of the baffle opens up groove structure；The sectional area of the feed inlet and the luminous tube
Sectional area is identical, and the spacing of the feed inlet, quantity are identical as the spacing of the luminous tube, quantity.
Beneficial effects of the present invention：
(1) detection device that this method uses, the position that will be operated with detect using the control box and the insulating box
It is separated, while independent enclosure space can effectively avoid the interference of other miscellaneous bacterias.
(2) detection device that this method uses, before detection, using three luminous tube packaging samples, using described
Clamping device is clamped fixation, hand-held without operator, can not only be carried out at the same time multiple detections, ensures detection by control
Accuracy reduces labour when operation.
(3) detection device that this method uses can be directly by it when carrying out the addition of buffer solution and fluorescein mixed liquor
It is placed in the inside in the packaging hole, the two is made in the way of extruding while entering the inside of multiple luminous tubes, significantly
Labour is reduced, the probability of error is reduced, improves the precision of detection.
(4) detection device that this method uses makes when entering the inside of the detection babinet using the motor
It is whole reciprocal shaking occur, it is ensured that internal mix is uniform, while before entry to the luminous tube carry out capping convenient for its into
Enter to the detection babinet and is used for quickly detecting.
Description of the drawings
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the structural schematic diagram for the detection device that this method uses；
Fig. 2 is the structural schematic diagram of charging mechanism shown in FIG. 1；
Fig. 3 is clamping device structural schematic diagram shown in Fig. 2；
Fig. 4 is retaining paw structural schematic diagram shown in Fig. 3；
Fig. 5 is control mechanism structural schematic diagram shown in FIG. 1；
Fig. 6 is the structural schematic diagram of testing agency shown in FIG. 1；
Fig. 7 is the structural schematic diagram of the side of baffle shown in fig. 6.
In figure：1, Qu Ye mechanisms, 11, liquid-collecting bottle, 12, fixed ring, 13, solenoid valve, 14, first time material head, 2, touch screen,
3, luminous tube, 4, control box, 41, handle hole, 5, charging mechanism, 51, pull rod, 52, connecting rod, 53, second time material head, 54, cover board,
55, connecting plate, 56, piston, 57, packaging hole, 58, packaging case, 6, insulating box, 61, transparency window, 62, handle, 7, clamping device,
71, motor, the 72, first electric pushrod, 73, connecting rod, 74, retaining paw, 741, clamping plate, 742, limiting plate, 743, second is electronic
Push rod, 744, plate tooth, 8, control mechanism, 81, by compressing switch, 82, movable plate, the 83, first torsional spring, 9, testing agency, 91, be in the light
Plate, 92, detection babinet, 93, feed inlet, the 94, second torsional spring, 95, baffle, 951, protrusion.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific implementation mode is closed, the present invention is further explained.
As shown in Figure 1, a kind of food safety detection method of the present invention, this method are examined using detection device
It surveys, the detection device includes Qu Ye mechanisms 1, touch screen 2, luminous tube 3, control box 4, charging mechanism 5, insulating box 6, clamping machine
Structure 7, control mechanism 8 and testing agency 9；The Qu Ye mechanisms 1 of the control box 4 being internally provided with for packaging and liquid separation,
The luminous tube 3 of 1 mating connection liquid separation packaging of the Qu Ye mechanisms；Being internally provided with for the control box 4 is added for auxiliary material
The charging mechanism 5, the charging mechanism 5 is connected the luminous tube 3, and is controlled described in the bottom of the charging mechanism 5
Mechanism 8 processed；The bottom of the control box 4 fixes the insulating box 6, and the insulating box 6 is internally provided with clamping and mobile institute
State the clamping device 7 of luminous tube 3；The insulating box 4 is internally provided with the testing agency 9, the Qu Ye mechanisms 1, institute
Charging mechanism 5, the insulating box 6, the clamping device 7, the control mechanism 8 and the testing agency 9 is stated to be electrically connected
The touch screen 2；
Detection method includes the following steps for this：
Step 1, Qu Ye mechanisms 1 extract detection sample；
The sample of extraction is sent into multiple luminous tubes 3 by step 2, the Qu Ye mechanisms 1 in step 1；
Luminous tube 3 in step 2 is moved at 5 position of charging mechanism by step 3, clamping device 7, charging mechanism 5 to
Buffer solution and fluorescein are added in luminous tube 3；Then, clamping device 7 rocks luminous tube 3, makes the solution in luminous tube 3
It is uniformly mixed；
Step 4, after step 3, luminous tube 3 is transported in testing agency 9 and is detected by clamping device 7.
Specifically, as shown in Figure 1, the Qu Ye mechanisms 1 include under liquid-collecting bottle 11, fixed ring 12, solenoid valve 13 and first
Material head 14, the fixed ring 12 are fixed on the side-walls of the control box 4, and the fixed ring 12 is connected together the liquid-collecting bottle
11；The bottom of the liquid-collecting bottle 11 is connected to the solenoid valve 13, and the solenoid valve 13 is electrically connected the touch screen 2；It is described
The bottom of solenoid valve 13 connects first time material head 14 by hose；There are three first time material head 14 is set, and described
Once the quantity, spacing of material head 14 are identical as 3 spacing of the luminous tube, quantity, and the sample that pretreatment is completed is placed in the collection
The solenoid valve 13 is opened in the inside of liquid bottle 11 when carrying out the detection of bacteria containing amount, and the sample that needs detect is made to pass through described the
Once material head 14 enters the inside of the luminous tube 3, realizes the liquid separation to sample, is carried out at the same time convenient for multiple detections, can be real
The control that reality is tested.
Specifically, as shown in Figure 1, the surface of the control box 4 opens up the handle hole 41 of symmetrical two circular hole structures,
And the insulating box 6 is welded in the bottom of the control box 4；The side of the insulating box 6 is equipped with transparency window 61, and the transparency window
611 are rotatablely connected the insulating box 6；Handle 62 is fixed in the side of the transparency window 61, can be to institute by the handle hole 41
It states and is operated manually inside control box 4, opening the transparency window 61 can be to being operated and being located inside the insulating box 6
Reason.
Specifically, as depicted in figs. 1 and 2, the charging mechanism 5 includes pull rod 51, connecting rod 52, second time material head 53, lid
Plate 54, connecting plate 55, piston 56, packaging hole 57 and packaging case 58；The packaging case 58 is fixed on the top of the control box 4,
And the inside of the packaging case 58 opens up two rows of packaging holes 57；The bottom in the packaging hole 57 is connected to second blanking
First 53, and second time material head 53 runs through the packaging case 58；The internal slide in the packaging hole 57 connects the piston
56, and the connecting rod 52 is fixed at the top of the piston 56；The connecting plate 55, and the company are fixed in the top of the connecting rod 52
The pull rod 51 is fixed at the top of fishplate bar 55；The pull rod 51 is slidably connected the cover board 54, and the cover board 54 is connected together
The packaging case 58, opens that the cover board 54 will need to add the buffer solution that adds respectively and fluorescein mixed liquor is placed in the collection
The inside for filling hole 57, covers the cover board 54, pull rod 51 described in pressable when being added, and the decline of the piston 56 is made to press
The packaging hole 57 makes that raw material to be added is needed to be fallen by second feed opening 53 under stress.
Specifically, as depicted in figs. 1 and 2, quantity, spacing and first time material head 14 in the single packaging hole 57
Quantity, spacing it is identical, and the height in the packaging hole 57, the height of the connecting rod 52 and bottom be open, hollow-core construction
The height of the cover board 54 is identical, in order to buffer solution and fluorescein mixed liquor is added inside each luminous tube 3.
Specifically, as shown in figures 1 and 3, the clamping device 7 includes motor 71, the first electric pushrod 72,73 and of connecting rod
Retaining paw 74, the motor 71 are embedded into the inside of the insulating box 6, and one end connection first electricity of the motor 71
Dynamic push rod 72；The connecting rod 73 is fixed in one end of first electric pushrod 72, and three institutes are fixed in the side of the connecting rod 73
Retaining paw 74 is stated, first electric pushrod 72 is opened and can be driven the luminous tube 3 to side movement, open the motor
71, which are allowed to reciprocating rotary, can be such that the luminous tube 3 shakes.
Specifically, as shown in Figure 3 and Figure 4, the quantity of the retaining paw 74, the number of spacing and first time material head 14
Amount, spacing are identical, and the retaining paw 74 includes clamping plate 741, limiting plate 742, the second electric pushrod 743 and plate tooth 744；Institute
The side that limiting plate 742 is fixed on the connecting rod 73 is stated, and second electric pushrod is fixed in the inside of the limiting plate 742
743；The side of second electric pushrod 743 connects the clamping plate 741, and the clamping plate 741 is rotatablely connected the limiting plate
742；The inside of the clamping plate 741 is equipped with the plate tooth 744；The clamping plate 741 uses semicircular structure, and the clamping plate
741 inside is equidistantly equipped with the plate tooth 744 of several rubber structures, opens when second motor 743 is allowed to stretch out i.e.
The clamping plate 741 can be made to open, the luminous tube 3 is put down, can make institute when second electric pushrod 743 is retracted
It states clamping plate 741 and closes and the luminous tube 3 be clamped.
Specifically, as shown in Figure 1 and Figure 5, the control mechanism 8 is set to the bottom end of the charging mechanism 5, the control machine
Structure 8 includes by 81, movable plate 82 and the first torsional spring 83 of compressing switch, and the movable plate 82 is connected to institute by first torsional spring 83
State the inside of insulating box 6, and the side of the movable plate 82 be equipped with it is described by compressing switch 81；It is described by 81 electric connections that compress switch
First electric pushrod 72 and the touch screen 2 can be closed when the clamping device 7, which is moved to, presses the movable plate 82
It closes first electric pushrod 72 and is allowed to stop motion, you can realize charging.
Specifically, as shown in figures 1 to 6, the testing agency 9 include light barrier 91, detection babinet 92, feed inlet 93,
Second torsional spring 94 and baffle 95, the detection babinet 92 are fixed on the inside of the insulating box 6, and the side of the detection babinet 92
Portion opens up the feed inlet 93；The baffle 95 is fixed at the top of the feed inlet 93, and the side of the feed inlet 93 passes through
Second torsional spring 94 connects the light barrier 91, when the luminous tube 3 is pushed by the clamping device 7, the luminous tube 3
Lid lid can be made to rotate due to the blocking of the baffle 95, with the entrance of the luminous tube 3, due to the feed inlet
93 limitation, makes lid close, open the light barrier 91 be sent to it is described detection babinet 92 inside be detected.
Specifically, as shown in Figure 6 and Figure 7, the baffle 95 uses inclination angle to be fixed on the feed inlet 93 for 30 ° -45 °
Top, and the bottom of the baffle 95 is equipped with several protrusions 951；The side of the baffle 95 opens up groove structure；It is described into
The sectional area of material mouth 93 is identical as the sectional area of the luminous tube 3, and the spacing of the feed inlet 93, quantity and the luminous tube
3 spacing, quantity are identical, for the ease of closing the lid of the luminous tube 3.
Sample is extracted by the Qu Ye mechanisms 1, is manually controlled by the touch screen 2, passes through the luminous tube 3
Packaging is carried out to the sample that needs detect, is manipulated by the control box 4, buffer solution is carried out by the charging mechanism 5
With the addition of fluorescein mixed liquor, suitable environment is improved by the insulating box 6, by the clamping device 7 to the hair
Light pipe 3 is gripped, and the progress of the charging mechanism 5 is controlled by the control mechanism 8, passes through the testing agency 9
It is detected；Specifically have：
(1) sample after pretreatment and culture is placed in the inside of the liquid-collecting bottle 11 first, in the inspection for carrying out bacteria containing amount
The clamping plate 741 can be made to close when survey, when second electric pushrod 743 being made to retract the luminous tube 3 is clamped, presss from both sides
The solenoid valve 13 is opened after holding, and so that the sample that needs detect is passed through first time material head 14 and is entered the luminous tube
The liquid separation to sample is realized in 3 inside, is carried out at the same time convenient for multiple detections.
(2) it then opens first electric pushrod 72 and can be driven the luminous tube 3 to side movement, when the clamping
Mechanism 7 can close first electric pushrod 72 when being moved to the pressing movable plate 82 and be allowed to stop motion, at this point, opening
The inside that the cover board 54 will need the buffer solution added respectively and fluorescein mixed liquor to be placed in the packaging hole 57 covers described
Cover board 54, pull rod 51 described in pressable when being added, so that the decline of the piston 56 is pressed the packaging hole 57 makes needs add
The raw material added is fallen by second feed opening 53 under stress.
(3) feed after the completion of, open the motor 71 be allowed to reciprocating rotary can make the luminous tube 3 shake, make inside
Mixing is abundant；Make first electric pushrod 72 continue to press on to move right, when reaching the side of the testing agency 9, institute
When stating clamping device 7 and being pushed into the luminous tube 3, the lid of the luminous tube 3 can make lid due to the blocking of the baffle 95
Son rotation, as the entrance of the luminous tube 3 makes lid close due to the limitation of the feed inlet 93, opens the light barrier
91 inside for being sent to the detection babinet 92 are detected, and the bacterium in food is measured using microbial atp biloluminescence method
Pollution level.
The food bacteria containing amount automatic tester of the present invention, using by the control box 4 and the insulating box 6 will operate with
The position of detection is separated, while independent enclosure space can effectively avoid the interference of other miscellaneous bacterias；Before detection, three are utilized
3 packaging sample of a luminous tube, fixation is clamped using the clamping device 7, hand-held without operator, not only can be simultaneously
Multiple detections are carried out, the accuracy of detection is ensured by control, reduce labour when operation；Carrying out buffer solution and fluorescein
When the addition of mixed liquor, the inside in the packaging hole 57 can be directly placed it in, the two is made in the way of extruding while being entered
To the inside of multiple luminous tubes 3, labour is substantially reduced, reduces the probability of error, improves the precision of detection；It is entering
When the inside of the detection babinet 92, entirety is set reciprocal shaking occur using the motor 71, it is ensured that internal mix is uniform,
Capping is carried out to the luminous tube 3 before entry simultaneously to enter the detection babinet 92 convenient for it and be used for quickly detecting.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the description in the above embodiment and specification only illustrates the present invention
Principle, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these variation and
Improvement is both fallen in the scope of protection of present invention.The claimed scope of the invention is by appended claims and its equivalent
Object defines.
Claims (10)
1. a kind of food safety detection method, it is characterised in that this method is detected using detection device, the detection device
Including Qu Ye mechanisms (1), touch screen (2), luminous tube (3), control box (4), charging mechanism (5), insulating box (6), clamping device
(7), control mechanism (8) and testing agency (9)；The control box (4) is internally provided with for taking liquid described in packaging and liquid separation
Mechanism (1), the luminous tube (3) of Qu Ye mechanisms (1) the mating connection liquid separation packaging；The inside of the control box (4) is set
Be useful for the charging mechanism (5) of auxiliary material addition, the charging mechanism (5) is connected the luminous tube (3), and it is described plus
Expect control mechanism (8) described in the bottom of mechanism (5)；The insulating box (6), and the perseverance are fixed in the bottom of the control box (4)
The clamping device (7) for being internally provided with clamping and the mobile luminous tube (3) of incubator (6)；The inside of the insulating box (4)
Equipped with the testing agency (9), the Qu Ye mechanisms (1), the charging mechanism (5), the insulating box (6), the clamping machine
Structure (7), the control mechanism (8) and the testing agency (9) are electrically connected the touch screen (2)；
Detection method includes the following steps for this：
Step 1, Qu Ye mechanisms (1) extract detection sample；
The sample of extraction is sent into multiple luminous tubes (3) by step 2, the Qu Ye mechanisms (1) in step 1；
Luminous tube (3) in step 2 is moved at charging mechanism (5) position by step 3, clamping device (7), charging mechanism
(5) addition buffer solution and fluorescein into luminous tube (3)；Then, clamping device (7) rocks luminous tube (3), makes to shine
The solution managed in (3) is uniformly mixed；
Step 4, after step 3, luminous tube (3) is transported in testing agency (9) and is detected by clamping device (7).
2. a kind of food safety detection method according to claim 1, it is characterised in that：The Qu Ye mechanisms (1) include
Liquid-collecting bottle (11), fixed ring (12), solenoid valve (13) and first time material head (14), the fixed ring (12) are fixed on the operation
The side-walls of case (4), and the fixed ring (12) is connected together the liquid-collecting bottle (11)；The bottom of the liquid-collecting bottle (11) is connected to
The solenoid valve (13), and the solenoid valve (13) is electrically connected the touch screen (2)；The bottom of the solenoid valve (13) passes through
Hose connects first time material head (14)；There are three first time material head (14) sets, and first time material head (14)
Quantity, spacing are identical as the luminous tube (3) spacing, quantity.
3. a kind of food safety detection method according to claim 1, it is characterised in that：The surface of the control box (4)
The handle hole (41) of symmetrical two circular hole structures is opened up, and the insulating box (6) is welded in the bottom of the control box (4)；
The side of the insulating box (6) is equipped with transparency window (61), and the transparency window (61) is rotatablely connected the insulating box (6)；It is described
Handle (62) is fixed in the side of transparency window (61).
4. a kind of food safety detection method according to claim 1, it is characterised in that：The charging mechanism (5) includes
Pull rod (51), connecting rod (52), second time material head (53), cover board (54), connecting plate (55), piston (56), packaging hole (57) sum aggregate
Mounted box (58)；The packaging case (58) is fixed on the top of the control box (4), and the inside of the packaging case (58) opens up two
Arrange the packaging hole (57)；The bottom of the packaging hole (57) is connected to second time material head (53), and second blanking
Head (53) runs through the packaging case (58)；The internal slide of the packaging hole (57) connects the piston (56), and the work
The connecting rod (52) is fixed at the top for filling in (56)；The connecting plate (55), and the connection are fixed in the top of the connecting rod (52)
The pull rod (51) is fixed at the top of plate (55)；The pull rod (51) is slidably connected the cover board (54), and the cover board (54)
Be connected together the packaging case (58).
5. a kind of food safety detection method according to claim 4, it is characterised in that：The single packaging hole (57)
Quantity, spacing it is identical as quantity, the spacing of first time material head (14), and the height in the packaging hole (57), the company
The height of bar (52) and bottom are that opening, the height of the cover board (54) of hollow-core construction are identical.
6. a kind of food safety detection method according to claim 1, it is characterised in that：The clamping device (7) includes
Motor (71), the first electric pushrod (72), connecting rod (73) and retaining paw (74), the motor (71) are embedded into the insulating box
(6) inside, and one end of the motor (71) connects first electric pushrod (72)；First electric pushrod (72)
The connecting rod (73) is fixed in one end, and three retaining paws (74) are fixed in the side of the connecting rod (73).
7. a kind of food safety detection method according to claim 6, it is characterised in that：The number of the retaining paw (74)
Amount, spacing are identical as quantity, the spacing of first time material head (14), and the retaining paw (74) includes clamping plate (741), limit
Position plate (742), the second electric pushrod (743) and plate tooth (744)；The limiting plate (742) is fixed on the side of the connecting rod (73)
Portion, and second electric pushrod (743) is fixed in the inside of the limiting plate (742)；The one of second electric pushrod (743)
Side connects the clamping plate (741), and the clamping plate (741) is rotatablely connected the limiting plate (742)；The clamping plate (741) it is interior
Side is equipped with the plate tooth (744)；The clamping plate (741) is using semicircular structure, and the inside of the clamping plate (741) is equidistantly set
There is the plate tooth (744) of several rubber structures.
8. a kind of food safety detection method according to claim 6, it is characterised in that：The control mechanism (8) is set to
The bottom end of the charging mechanism (5), the control mechanism (8) include by compress switch (81), movable plate (82) and the first torsional spring
(83), the movable plate (82) is connected to the inside of the insulating box (6), and the movable plate by first torsional spring (83)
(82) side is equipped with described by compress switch (81)；It is described by compress switch (81) be electrically connected first electric pushrod (72) and
The touch screen (2).
9. a kind of food safety detection method according to claim 1, it is characterised in that：The testing agency (9) includes
Light barrier (91), detection babinet (92), feed inlet (93), the second torsional spring (94) and baffle (95), the detection babinet (92) are solid
The feed inlet (93) is opened up due to the side of the inside of the insulating box (6), and the detection babinet (92)；The feed inlet
(93) baffle (95) is fixed at top, and the side of the feed inlet (93) passes through described in second torsional spring (94) connection
Light barrier (91).
10. a kind of food safety detection method according to claim 9, it is characterised in that：The baffle (95), which uses, to be inclined
Angle is 30 ° -45 ° tops for being fixed on the feed inlet (93), and the bottom of the baffle (95) is equipped with several protrusions
(951)；The side of the baffle (95) opens up groove structure；The sectional area of the feed inlet (93) and the luminous tube (3)
Sectional area is identical, and the spacing of the feed inlet (93), quantity are identical as spacing, the quantity of the luminous tube (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810322386.6A CN108318466B (en) | 2018-04-11 | 2018-04-11 | Food safety detection method |
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| CN201810322386.6A CN108318466B (en) | 2018-04-11 | 2018-04-11 | Food safety detection method |
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| CN108318466B CN108318466B (en) | 2020-09-22 |
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| CN108318466B (en) | 2020-09-22 |
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Effective date of registration: 20200826 Address after: 674200 Yongsheng Industrial Park, Yongsheng County, Lijiang City, Yunnan Province Applicant after: LIJIANG SANCHUAN INDUSTRIAL GROUP Co.,Ltd. Address before: 310058 Zhejiang University, 866 Tong Road, Hangzhou, Zhejiang, Yuhang, Xihu District Applicant before: Fan Xuehua |
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