CN106190823B - A kind of E. coli detection device in aquatic products - Google Patents
A kind of E. coli detection device in aquatic products Download PDFInfo
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- CN106190823B CN106190823B CN201610556531.8A CN201610556531A CN106190823B CN 106190823 B CN106190823 B CN 106190823B CN 201610556531 A CN201610556531 A CN 201610556531A CN 106190823 B CN106190823 B CN 106190823B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention discloses the E. coli detection devices in a kind of aquatic products, including detection device ontology, the detection device ontology includes detection case and controller, the detection case includes control room and culture sensing chamber, the control room is separated by fixed plate and culture sensing chamber, electric rotating machine is installed in the control room, first motor, second motor, thermoregulator and humidistat, culture detection interior is equipped with homogenizer, fermentation tube and fixed disk, the fermentation tube is mounted on by support column in fixed plate, the fixed disk is mounted on by supporting rod in fixed plate, the fixed disk is equipped with culture dish and detector, the electric rotating machine is electrically connected with the swingle in homogenizer, the swingle is equipped with paddle.E. coli detection device in the aquatic products, in order to uniform dilute solution, is conducive to reduce the error examined due to homogenizer.
Description
Technical field
The present invention relates to testing equipment field, the E. coli detection device in specially a kind of aquatic products.
Background technology
The general designation of the animals and plants and its converted products of aquatic products ocean and fresh water fishery production, the microorganism inspection in aquatic products
Proved recipe method is that aquatic product quality manages essential important component, can be effectively prevented or reduce zoonosis
Generation, ensure the health of the people.Contain Escherichia coli in aquatic products, Escherichia coli be in people and many animal intestinal tracts most
A kind of most bacterium of main and quantity, main parasitic is in big enteral.When it invades some positions of human body, it can cause to infect, such as
Peritonitis, cholecystitis, cystitis and diarrhea etc..Symptom of the people after ehec infection is stomachache, vomiting, diarrhea and fever.
Infection may be fatefulue, especially to child and old man, for this purpose, we provide the E. coli detection in a kind of aquatic products
Device.
Invention content
The purpose of the present invention is to provide the E. coli detection devices in a kind of aquatic products, to solve above-mentioned background technology
The problem of middle proposition.
To achieve the above object, the present invention provides the following technical solutions：A kind of E. coli detection device in aquatic products,
Including detection device ontology, the detection device ontology includes detection case and controller, and the detection case includes control room and training
Sensing chamber is supported, the control room is separated by fixed plate and culture sensing chamber, and electric rotating machine, first are equipped in the control room
Motor, the second motor, thermoregulator and humidistat, culture detection interior are equipped with homogenizer, fermentation tube and consolidate
Price fixing, the fermentation tube are mounted on by support column in fixed plate, and the fixed disk is mounted on by supporting rod in fixed plate, institute
It states fixed disk and is equipped with culture dish and detector, the electric rotating machine is electrically connected with the swingle in homogenizer, the rotation
Bar is equipped with paddle, and the homogenizer is connect by catheter with fermentation tube, and drawing liquid pump is equipped in the catheter.
Preferably, the homogenizer passes through the first injection of the first liquid injection pipe and the second liquid injection pipe and detection case upper end respectively
Mouth is connected with the second injection port, and the fermentation tube side is equipped with the first rotation axis, and first rotation axis is equipped with rotor plate, institute
Fermentation tube is stated respectively to connect with the third injection port of detection case upper end and the 4th injection port by third liquid injection pipe and the 4th liquid injection pipe
It connects.
Preferably, the indoor upper end of culture detection is equipped with sterilizer and telescopic hydraulic bar, in the telescopic hydraulic bar
End is equipped with the 5th injection port, is hollow form in the telescopic hydraulic bar, and the telescopic hydraulic bar passes through the second rotation axis and inoculation
Stick connects.
Preferably, the detection case upper end is equipped with fixed link and hydraulic device, and the fixed link upper end is equipped with alarm
Device.
Preferably, the controller outer surface is equipped with display screen, control switch and operator control panel, is equipped in the controller
Central processing unit, alarm module, display module and timer, the timer are electrically connected by alarm module and alarm,
The detector is electrically connected with the central processing unit, and the central processing unit is electrically connected by display module and display screen.
Preferably, chamber door is equipped with by hinge on the outside of the detection case, the hinge is equipped with fixed screw, the case
Door is equipped with handle.
Compared with prior art, the beneficial effects of the invention are as follows：E. coli detection device in the aquatic products, due to equal
Matter device is conducive to reduce the error examined, due to controlling indoor thermoregulator and humidity tune in order to uniform dilute solution
Device is saved, the temperature and humidity in detection case can be maintained in a certain range, to be conducive to the culture and detection of aquatic products, by
Indoor sterilizer is detected in culture, it can be ensured that the sterile culture environment in detection case can due to the timer in controller
To control the detection time of culture dish, alarmed by alarm when reaching the predetermined time.
Description of the drawings
Fig. 1 is overall structure of the present invention；
Fig. 2 is shell mechanism schematic diagram of the present invention；
Fig. 3 is controller internal structure schematic diagram of the present invention；
In figure：1 detection device ontology, 2 control rooms, 3 culture sensing chamber, 4 first liquid injection pipes, 5 second liquid injection pipes, 6 homogeneous
Device, 7 drawing liquid pumps, 8 catheters, 9 paddles, 10 swingles, 11 fixed plates, 12 electric rotating machines, 13 thermoregulators, 14 supports
Column, 15 fermentation tubes, 16 first motors, 17 rotor plates, 18 humidistats, 19 first rotation axis, 20 second motors, 21 are fixed
Disk, 22 culture dishes, 23 supporting rods, 24 second rotation axis, 25 detectors, 26 alarm modules, 27 operator control panels, 28 control switches, 29
Display screen, 30 controllers, 31 loop-carriers, 32 hydraulic devices, 33 telescopic hydraulic bars, 34 the 5th injection ports, 35 sterilizers, 36 are fixed
Bar, 37 alarms, 38 third liquid injection pipes, 39 the 4th injection ports, 40 the 4th liquid injection pipes, 41 third injection ports, 42 detection cases, 43
Two injection ports, 44 first injection ports, 45 chamber doors, 46 fixed screws, 47 hinges, 48 handles, 49 timers, 50 display modules, 51
Central processing unit.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
- 3 are please referred to Fig.1, the present invention provides a kind of technical solution：A kind of E. coli detection device in aquatic products, packet
Detection device ontology 1 is included, the detection device ontology 1 includes detection case 42 and controller 30, and the detection case 42 includes control
Room 2 and culture sensing chamber 3, the control room 2 are separated by fixed plate 11 and culture sensing chamber 3, are equipped in the control room 2
Electric rotating machine 12, first motor 16, the second motor 20, thermoregulator 13 and humidistat 18, in the culture sensing chamber 3
Homogenizer 6, fermentation tube 15 and fixed disk 21, the fermentation tube 15 are installed to be mounted in fixed plate 11 by support column 14, institute
Fixed disk 21 to be stated to be mounted in fixed plate 11 by supporting rod 23, the fixed disk 21 is equipped with culture dish 22 and detector 25,
The electric rotating machine 12 is electrically connected with the swingle 10 in homogenizer 6, and the swingle 10 is equipped with paddle 9, described equal
Matter device 6 is connect by catheter 8 with fermentation tube 15, and drawing liquid pump 7 is equipped in the catheter 8.
The homogenizer 6 passes through the first injection port of the first liquid injection pipe 4 and the second liquid injection pipe 2 and 42 upper end of detection case respectively
44 and second injection port 43 connect, 15 side of the fermentation tube is equipped with the first rotation axis 19, and first rotation axis 19 is equipped with
Rotor plate 17, the fermentation tube 15 are noted by third liquid injection pipe 38 and the 4th liquid injection pipe 40 and the third of 42 upper end of detection case respectively
Loophole 41 and the 4th injection port 39 connect, and upper end is equipped with sterilizer 35 and telescopic hydraulic bar 33, institute in the culture sensing chamber 3
It states 33 upper end of telescopic hydraulic bar and is equipped with the 5th injection port 34, be hollow form, the telescopic hydraulic bar in the telescopic hydraulic bar 33
33 are connect by the second rotation axis 24 with loop-carrier 31, and 42 upper end of the detection case is equipped with fixed link 36 and hydraulic device 32,
36 upper end of the fixed link is equipped with alarm 37, and 30 outer surface of the controller is equipped with display screen 29,28 and of control switch
Operator control panel 27, the controller 30 is interior equipped with central processing unit 51, alarm module 26, display module 50 and timer 49, described
Timer 49 is electrically connected by alarm module 26 and alarm 37, and the detector 25 is electrically connected with central processing unit 51,
The central processing unit 51 is electrically connected by display module 50 and display screen 29, and 42 outside of the detection case is pacified by hinge 47
Equipped with chamber door 45, the hinge 47 is equipped with fixed screw 46, and the chamber door 45 is equipped with handle 48, due to homogenizer 6, so as to
In uniform dilute solution, be conducive to reduce the error examined, due to the thermoregulator 13 and humidistat in control room 2
18, the temperature and humidity in detection case can be maintained in a certain range, to be conducive to the culture and detection of aquatic products, due to
Cultivate the sterilizer 35 in sensing chamber 3, it can be ensured that the sterile culture environment in detection case 42, due to the timing in controller 30
Device 49 can control the detection time of culture dish 22, be alarmed by alarm 37 when reaching the predetermined time.
Operation principle：E. coli detection device in the aquatic products notes sterile saline and aquatic products lapping liquid
Enter into homogenizer 6, makes uniform dilution.Measuring samples are inoculated in fermentation tube 15 again, are then injected to fermentation tube 15
Lactose cholate can then be reported as coliform feminine gender, if any aerogenesis person, will produce such as all lactose cholate fermentation tube not aerogenesis
The fermentation tube 15 of gas on culture dish 22, is cultivated by 31 transferred species of loop-carrier, is carried out to incubation time by timer 49
Periodically, it is alarmed by alarm 37 when reaching the predetermined time, reminds people, then take out, observe colonial morphology, and do
Gram's staining and verification test.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (2)
1. the E. coli detection device in a kind of aquatic products, including detection device ontology（1）, it is characterised in that：The detection
Device body（1）Including detection case（42）And controller（30）, the detection case（42）Including control room（2）With culture sensing chamber
（3）, the detection case（42）Upper end is equipped with fixed link（36）And hydraulic device（32）, the fixed link（36）Upper end is equipped with
Alarm（37）, the culture sensing chamber（3）Interior upper end is equipped with sterilizer（35）And telescopic hydraulic bar（33）, the flexible liquid
Compression bar（33）Upper end is equipped with the 5th injection port（34）, the telescopic hydraulic bar（33）Interior is hollow form, the telescopic hydraulic bar
（33）Pass through the second rotation axis（24）With loop-carrier（31）Connection, the control room（2）Pass through fixed plate（11）It is detected with culture
Room（3）Separate, the control room（2）Electric rotating machine is inside installed（12）, first motor（16）, the second motor（20）, temperature tune
Save device（13）And humidistat（18）, the culture sensing chamber（3）Homogenizer is inside installed（6）, fermentation tube（15）And fixation
Disk（21）, the fermentation tube（15）Pass through support column（14）Mounted on fixed plate（11）On, the fixed disk（21）Pass through support
Bar（23）Mounted on fixed plate（11）On, the fixed disk（21）It is equipped with culture dish（22）And detector（25）, the homogeneous
Device（6）Pass through the first liquid injection pipe respectively（4）With the second liquid injection pipe（5）With detection case（42）First injection port of upper end（44）With
Two injection ports（43）Connection, the fermentation tube（15）Side is equipped with the first rotation axis（19）, first rotation axis（19）Installation
There is rotor plate（17）, the fermentation tube（15）Pass through third liquid injection pipe respectively（38）With the 4th liquid injection pipe（40）With detection case（42）
The third injection port of upper end（41）With the 4th injection port（39）Connection, the electric rotating machine（12）With homogenizer（6）In rotation
Bar（10）It is electrically connected, the swingle（10）Paddle is installed（9）, the homogenizer（6）Pass through catheter（8）With fermentation
Pipe（15）Connection, the catheter（8）Drawing liquid pump is inside installed（7）, the controller（30）Outer surface is equipped with display screen
（29）, control switch（28）And operator control panel（27）, the controller（30）It is interior to be equipped with central processing unit（51）, alarm module
（26）, display module（50）And timer（49）, the timer（49）Pass through alarm module（26）With alarm（37）Electrically
Connection, the detector（25）With central processing unit（51）It is electrically connected, the central processing unit（51）Pass through display module
（50）With display screen（29）It is electrically connected.
2. the E. coli detection device in a kind of aquatic products according to claim 1, it is characterised in that：The detection case
（42）Outside passes through hinge（47）Chamber door is installed（45）, the hinge（47）It is equipped with fixed screw（46）, the chamber door
（45）It is equipped with handle（48）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610556531.8A CN106190823B (en) | 2016-07-15 | 2016-07-15 | A kind of E. coli detection device in aquatic products |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610556531.8A CN106190823B (en) | 2016-07-15 | 2016-07-15 | A kind of E. coli detection device in aquatic products |
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| Publication Number | Publication Date |
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| CN106190823A CN106190823A (en) | 2016-12-07 |
| CN106190823B true CN106190823B (en) | 2018-11-02 |
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| CN201610556531.8A Active CN106190823B (en) | 2016-07-15 | 2016-07-15 | A kind of E. coli detection device in aquatic products |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112899138A (en) * | 2021-01-11 | 2021-06-04 | 浙江省海洋水产研究所 | Device and method for determining microbial colony number |
| CN112899148A (en) * | 2021-01-11 | 2021-06-04 | 浙江省海洋水产研究所 | Microbial colony count measuring device and measuring method |
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| CN201165524Y (en) * | 2007-12-14 | 2008-12-17 | 姜世民 | Multi-culture medium automatic bacteria isolated culture instrument |
| CN103194385A (en) * | 2013-03-14 | 2013-07-10 | 华珊微生物科技有限公司 | Microbial sample processing system |
| CN204661647U (en) * | 2015-03-25 | 2015-09-23 | 山东鑫科生物科技股份有限公司 | A kind of sample disposal inoculation culture device |
| CN205188296U (en) * | 2015-10-20 | 2016-04-27 | 武汉迪艾斯科技有限公司 | Automatic change kind of formula blood and cultivate appearance |
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2016
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1650291A1 (en) * | 2004-10-25 | 2006-04-26 | Kawasaki Jukogyo Kabushiki Kaisha | Automatic cell cultivation apparatus utilizing autoclave sterilization and method for using the same |
| JP2006174828A (en) * | 2004-11-29 | 2006-07-06 | Olympus Corp | Biological sample-culturing and observing system, incubator box, supply means, and culture container |
| WO2006122089A2 (en) * | 2005-05-09 | 2006-11-16 | Isolagen Technologies, Inc. | Cell growth system |
| CN201165524Y (en) * | 2007-12-14 | 2008-12-17 | 姜世民 | Multi-culture medium automatic bacteria isolated culture instrument |
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