CN106190823A - A kind of E. coli detection device in aquatic products - Google Patents

A kind of E. coli detection device in aquatic products Download PDF

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Publication number
CN106190823A
CN106190823A CN201610556531.8A CN201610556531A CN106190823A CN 106190823 A CN106190823 A CN 106190823A CN 201610556531 A CN201610556531 A CN 201610556531A CN 106190823 A CN106190823 A CN 106190823A
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detection device
aquatic products
homogenizer
coli
fermentation tube
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CN201610556531.8A
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CN106190823B (en
Inventor
金雷
蒋雪燕
陈瑜
鲍静姣
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Zhejiang Marine Fisheries Research Institute
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Zhejiang Marine Fisheries Research Institute
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Molecular Biology (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses the E. coli detection device in a kind of aquatic products, including detection device body, described detection device body includes detection case and controller, described detection case includes control room and cultivates sensing chamber, described control room is separated with cultivating sensing chamber by fixed plate, in described control room, electric rotating machine is installed, first motor, second motor, thermoregulator and humidistat, in described cultivation sensing chamber, homogenizer is installed, fermentation tube and fixed disk, described fermentation tube is arranged in fixed plate by support column, described fixed disk is arranged in fixed plate by support bar, described fixed disk is provided with culture dish and detector, described electric rotating machine is electrically connected with the swingle in homogenizer, described swingle is provided with paddle.E. coli detection device in these aquatic products, due to homogenizer, in order to uniformly dilute solution, is conducive to reducing the error of inspection.

Description

A kind of E. coli detection device in aquatic products
Technical field
The present invention relates to detect apparatus field, the E. coli detection device in a kind of aquatic products.
Background technology
Animals and plants that aquatic products ocean and fresh water fishery produce and the general designation of converted products, the microorganism inspection in aquatic products Proved recipe method is that aquatic product quality manages requisite important component part, can be effectively prevented or reduce zoonosis Generation, ensure the people healthy.Containing escherichia coli in aquatic products, escherichia coli be in people and many animal intestinals Main and that quantity is most a kind of antibacterials, main parasitic is at big enteral.When it invades some positions of human body, infection can be caused, as Peritonitis, cholecystitis, cystitis and diarrhoea etc..People's symptom after ehec infection is for have a stomachache, vomit, suffer from diarrhoea and to generate heat. Infection is probably fatefulue, especially to child and old man, to this end, we provide the E. coli detection in a kind of aquatic products Device.
Summary of the invention
It is an object of the invention to provide the E. coli detection device in a kind of aquatic products, to solve above-mentioned background technology The problem of middle proposition.
For achieving the above object, the present invention provides following technical scheme: the E. coli detection device in a kind of aquatic products, Including detection device body, described detection device body includes that detection case and controller, described detection case include control room and training Support sensing chamber, described control room by fixed plate with cultivate sensing chamber separate, be provided with in described control room electric rotating machine, first Motor, the second motor, thermoregulator and humidistat, be provided with homogenizer, fermentation tube and consolidate in described cultivation sensing chamber Price fixing, described fermentation tube is arranged in fixed plate by support column, and described fixed disk is arranged in fixed plate by support bar, institute Stating fixed disk and be provided with culture dish and detector, described electric rotating machine is electrically connected with the swingle in homogenizer, described rotation Bar is provided with paddle, and described homogenizer is connected with fermentation tube by catheter, is provided with drawing liquid pump in described catheter.
Preferably, described homogenizer is injected with the first of detection case upper end by the first liquid injection pipe and the second liquid injection pipe respectively Mouth and the second injection port connect, and described fermentation tube side is provided with the first rotary shaft, and described first rotary shaft is provided with rotor plate, institute State fermentation tube to be connected by the 3rd liquid injection pipe and the 4th liquid injection pipe and the 3rd injection port of detection case upper end and the 4th injection port respectively Connect.
Preferably, in described cultivation sensing chamber, upper end is provided with steriliser and telescopic hydraulic bar, on described telescopic hydraulic bar End is provided with the 5th injection port, is hollow form in described telescopic hydraulic bar, and described telescopic hydraulic bar passes through the second rotary shaft and inoculation Rod connects.
Preferably, described detection case upper end is provided with fixing bar and hydraulic means, and described fixing bar upper end is provided with warning Device.
Preferably, described controller outer surface is provided with display screen, controls switch and operation screen, is provided with in described controller Central processing unit, alarm module, display module and timer, described timer is electrically connected with alarm by alarm module, Described detector is electrically connected with the central processing unit, and described central processing unit is electrically connected with display screen by display module.
Preferably, being provided with chamber door by hinge outside described detection case, described hinge is provided with fixed screw, described case Door is provided with handle.
Compared with prior art, the invention has the beneficial effects as follows: the E. coli detection device in these aquatic products, due to all Matter device, in order to uniformly dilute solution, is conducive to reducing the error of inspection, owing to the thermoregulator in control room and humidity are adjusted Joint device, can maintain in certain scope by the temperature and humidity in detection case, beneficially the cultivation of aquatic products and detection, by Steriliser in cultivating sensing chamber, it can be ensured that the aseptic culture environment in detection case, due to the timer in controller, can To control the detection time of culture dish, reported to the police by alarm when arriving the scheduled time.
Accompanying drawing explanation
Fig. 1 is overall structure schematic diagram of the present invention;
Fig. 2 is shell mechanism schematic diagram of the present invention;
Fig. 3 is controller internal structure schematic diagram of the present invention;
In figure: 1 detection device body, 2 control rooms, 3 cultivate sensing chamber, 4 first liquid injection pipes, 5 second liquid injection pipes, 6 homogenizers, 7 Drawing liquid pump, 8 catheters, 9 paddles, 10 swingles, 11 fixed plates, 12 electric rotating machines, 13 thermoregulators, 14 support columns, 15 Fermentation tube, 16 first motors, 17 rotor plates, 18 humidistats, 19 first rotary shafts, 20 second motors, 21 fixed disks, 22 trainings Support ware, 23 support bars, 24 second rotary shafts, 25 detectors, 26 alarm modules, 27 operation screen, 28 control switches, 29 display screens, 30 controllers, 31 loop carriers, 32 hydraulic means, 33 telescopic hydraulic bars, 34 the 5th injection ports, 35 sterilisers, 36 fix bar, 37 report Alert device, 38 the 3rd liquid injection pipes, 39 the 4th injection ports, 40 the 4th liquid injection pipes, 41 the 3rd injection ports, 42 detection cases, 43 second injections Mouth, 44 first injection ports, 45 chamber doors, 46 fixed screws, 47 hinges, 48 handles, 49 timers, 50 display modules, 51 centre Reason device.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments wholely.Based on Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under not making creative work premise Embodiment, broadly falls into the scope of protection of the invention.
Referring to Fig. 1-3, the present invention provides a kind of technical scheme: the E. coli detection device in a kind of aquatic products, bag Including detection device body 1, described detection device body 1 includes that detection case 42 and controller 30, described detection case 42 include controlling Room 2 and cultivation sensing chamber 3, described control room 2 is separated with cultivating sensing chamber 3 by fixed plate 11, is provided with in described control room 2 Electric rotating machine the 12, first motor the 16, second motor 20, thermoregulator 13 and humidistat 18, in described cultivation sensing chamber 3 Being provided with homogenizer 6, fermentation tube 15 and fixed disk 21, described fermentation tube 15 is arranged in fixed plate 11 by support column 14, institute Stating fixed disk 21 to be arranged in fixed plate 11 by support bar 23, described fixed disk 21 is provided with culture dish 22 and detector 25, Described electric rotating machine 12 is electrically connected with the swingle 10 in homogenizer 6, and described swingle 10 is provided with paddle 9, described all Matter device 6 is connected with fermentation tube 15 by catheter 8, is provided with drawing liquid pump 7 in described catheter 8.
Described homogenizer 6 is respectively by the first liquid injection pipe 4 and the second liquid injection pipe 2 and the first injection port of detection case 42 upper end 44 and second injection port 43 connect, described fermentation tube 15 side is provided with the first rotary shaft 19, and described first rotary shaft 19 is provided with Rotor plate 17, described fermentation tube 15 is respectively by the 3rd liquid injection pipe 38 and the 4th liquid injection pipe 40 and the 3rd note of detection case 42 upper end Loophole 41 and the 4th injection port 39 connect, and in described cultivation sensing chamber 3, upper end is provided with steriliser 35 and telescopic hydraulic bar 33, institute State telescopic hydraulic bar 33 upper end and be provided with the 5th injection port 34, be hollow form in described telescopic hydraulic bar 33, described telescopic hydraulic bar 33 are connected with loop carrier 31 by the second rotary shaft 24, and described detection case 42 upper end is provided with fixing bar 36 and hydraulic means 32, Described fixing bar 36 upper end is provided with alarm 37, and described controller 30 outer surface is provided with display screen 29, controls switch 28 and Operation screen 27, is provided with central processing unit 51, alarm module 26, display module 50 and timer 49 in described controller 30, described Timer 49 is electrically connected with alarm 37 by alarm module 26, and described detector 25 is electrically connected with central processing unit 51, Described central processing unit 51 is electrically connected with display screen 29 by display module 50, is pacified by hinge 47 outside described detection case 42 Equipped with chamber door 45, described hinge 47 is provided with fixed screw 46, and described chamber door 45 is provided with handle 48, due to homogenizer 6, in order to In uniform dilute solution, be conducive to reducing the error of inspection, due to the thermoregulator 13 in control room 2 and humidistat 18, can the temperature and humidity in detection case be maintained in certain scope, beneficially the cultivation of aquatic products and detection, due to Cultivate the steriliser 35 in sensing chamber 3, it can be ensured that the aseptic culture environment in detection case 42, due to the timing in controller 30 Device 49, can control the detection time of culture dish 22, is reported to the police by alarm 37 when arriving the scheduled time.
Operation principle: the E. coli detection device in these aquatic products, notes sterile saline and aquatic products lapping liquid Enter in homogenizer 6, do uniformly diluent.Measuring samples is inoculated in fermentation tube 15 again, then injects to fermentation tube 15 Lactose cholate, such as all lactose cholate fermentation tube the most not aerogenesis, then can be reported as coliform negative, if any aerogenesis person, will produce The fermentation tube 15 of gas by loop carrier 31 transferred species, is cultivated, is carried out incubation time by timer 49 on culture dish 22 Regularly, reported to the police by alarm 37 when arriving the scheduled time, remind people, then take out, observe colonial morphology, and do Gram’s staining and verification test.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, permissible Understand and these embodiments can be carried out multiple change without departing from the principles and spirit of the present invention, revise, replace And modification, the scope of the present invention be defined by the appended.

Claims (6)

1. the E. coli detection device in aquatic products, including detection device body (1), it is characterised in that: described detection Device body (1) includes detection case (42) and controller (30), and described detection case (42) includes control room (2) and cultivates sensing chamber (3), described control room (2) is separated with cultivating sensing chamber (3) by fixed plate (11), and described control room is provided with rotation in (2) Motor (12), the first motor (16), the second motor (20), thermoregulator (13) and humidistat (18), described cultivation is examined Being provided with homogenizer (6), fermentation tube (15) and fixed disk (21) in surveying room (3), described fermentation tube (15) passes through support column (14) Being arranged in fixed plate (11), described fixed disk (21) is arranged in fixed plate (11) by support bar (23), described fixed disk (21) culture dish (22) and detector (25), described electric rotating machine (12) and swingle (10) electricity in homogenizer (6) it are provided with Property connect, described swingle (10) is provided with paddle (9), and described homogenizer (6) is by catheter (8) and fermentation tube (15) even Connect, drawing liquid pump (7) is installed in described catheter (8).
E. coli detection device in a kind of aquatic products the most according to claim 1, it is characterised in that: described homogenizer (6) respectively by the first liquid injection pipe (4) and the second liquid injection pipe (2) and first injection port (44) and second of detection case (42) upper end Injection port (43) connects, and described fermentation tube (15) side is provided with the first rotary shaft (19), and described first rotary shaft (19) is provided with Rotor plate (17), described fermentation tube (15) is respectively by the 3rd liquid injection pipe (38) and the 4th liquid injection pipe (40) and detection case (42) 3rd injection port (41) of end and the 4th injection port (39) connect.
E. coli detection device in a kind of aquatic products the most according to claim 1, it is characterised in that: described cultivation is examined Surveying room (3) interior upper end and be provided with steriliser (35) and telescopic hydraulic bar (33), described telescopic hydraulic bar (33) upper end is provided with the 5th Injection port (34), is hollow form in described telescopic hydraulic bar (33), and described telescopic hydraulic bar (33) passes through the second rotary shaft (24) It is connected with loop carrier (31).
E. coli detection device in a kind of aquatic products the most according to claim 1, it is characterised in that: described detection case (42) upper end is provided with fixing bar (36) and hydraulic means (32), and described fixing bar (36) upper end is provided with alarm (37).
5. according to the E. coli detection device in a kind of aquatic products described in claim 1 or 4, it is characterised in that: described control Device processed (30) outer surface is provided with display screen (29), controls switch (28) and operation screen (27), and described controller is provided with in (30) Central processing unit (51), alarm module (26), display module (50) and timer (49), described timer (49) is by warning mould Block (26) is electrically connected with alarm (37), and described detector (25) is electrically connected with central processing unit (51), described centre Reason device (51) is electrically connected with display screen (29) by display module (50).
E. coli detection device in a kind of aquatic products the most according to claim 1, it is characterised in that: described detection case (42) outside is provided with chamber door (45) by hinge (47), and described hinge (47) is provided with fixed screw (46), described chamber door (45) handle (48) it is provided with.
CN201610556531.8A 2016-07-15 2016-07-15 A kind of E. coli detection device in aquatic products Active CN106190823B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112899148A (en) * 2021-01-11 2021-06-04 浙江省海洋水产研究所 Microbial colony count measuring device and measuring method
CN112899138A (en) * 2021-01-11 2021-06-04 浙江省海洋水产研究所 Device and method for determining microbial colony number

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JP2006174828A (en) * 2004-11-29 2006-07-06 Olympus Corp Biological sample-culturing and observing system, incubator box, supply means, and culture container
WO2006122089A2 (en) * 2005-05-09 2006-11-16 Isolagen Technologies, Inc. Cell growth system
CN201165524Y (en) * 2007-12-14 2008-12-17 姜世民 Multi-culture medium automatic bacteria isolated culture instrument
CN103194385A (en) * 2013-03-14 2013-07-10 华珊微生物科技有限公司 Microbial sample processing system
CN204661647U (en) * 2015-03-25 2015-09-23 山东鑫科生物科技股份有限公司 A kind of sample disposal inoculation culture device
CN205188296U (en) * 2015-10-20 2016-04-27 武汉迪艾斯科技有限公司 Automatic change kind of formula blood and cultivate appearance

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1650291A1 (en) * 2004-10-25 2006-04-26 Kawasaki Jukogyo Kabushiki Kaisha Automatic cell cultivation apparatus utilizing autoclave sterilization and method for using the same
JP2006174828A (en) * 2004-11-29 2006-07-06 Olympus Corp Biological sample-culturing and observing system, incubator box, supply means, and culture container
WO2006122089A2 (en) * 2005-05-09 2006-11-16 Isolagen Technologies, Inc. Cell growth system
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CN204661647U (en) * 2015-03-25 2015-09-23 山东鑫科生物科技股份有限公司 A kind of sample disposal inoculation culture device
CN205188296U (en) * 2015-10-20 2016-04-27 武汉迪艾斯科技有限公司 Automatic change kind of formula blood and cultivate appearance

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112899148A (en) * 2021-01-11 2021-06-04 浙江省海洋水产研究所 Microbial colony count measuring device and measuring method
CN112899138A (en) * 2021-01-11 2021-06-04 浙江省海洋水产研究所 Device and method for determining microbial colony number

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