CN106179549A - A kind of micro-fluidic chip and application thereof - Google Patents
A kind of micro-fluidic chip and application thereof Download PDFInfo
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- CN106179549A CN106179549A CN201610685846.2A CN201610685846A CN106179549A CN 106179549 A CN106179549 A CN 106179549A CN 201610685846 A CN201610685846 A CN 201610685846A CN 106179549 A CN106179549 A CN 106179549A
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- 239000007788 liquid Substances 0.000 claims abstract description 112
- 238000000034 method Methods 0.000 claims abstract description 15
- 239000002699 waste material Substances 0.000 claims description 21
- 238000001514 detection method Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 3
- 230000000977 initiatory effect Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 abstract description 88
- 239000013068 control sample Substances 0.000 abstract description 6
- 238000010276 construction Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 description 8
- 238000013461 design Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
- B01F33/3017—Mixing chamber
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0883—Serpentine channels
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
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Abstract
The invention discloses a kind of micro-fluidic chip, multiple parallel reaction channel sample can be realized and automatically throw in and the control of staged concentration.Micro-fluidic chip rotates on centrifugal force platform, by the adjustment to speed so that the sample in sample room enters helical duct, and flows in helical duct；Control sample by the size of regulation centrifugal force and the dimensional structure of metering valve and enter order and the volume of mixed liquid chamber.The invention also discloses method based on this device, and the microfluidic chip structure of corresponding plurality of liquid, including multiple sample rooms, a plurality of helical duct.The present invention is by the centrifugal force size suffered by regulation micro-fluidic chip, it is achieved that the Multi-example on micro-fluidic chip is thrown in and staged concentration control apparatus automatically, and simple in construction is easy to operate.
Description
Technical field
The present invention relates to microfluidic art, the multiple parallel reaction channel sample on a kind of micro-fluidic chip is certainly
Move input and staged concentration control apparatus and method.
Background technology
Centrifugal power-actuated micro-fluidic chip, is to utilize micro-fluidic chip produced centrifugal masterpiece when moving in a circle
For the driving force of liquid stream, regulate by changing chip rotary speed with designing different channel configurations and control the dynamic of fluid
Characteristic.It is a kind of technology more unique in micro-fluidic actuation techniques that centrifugal force drives, compared with other micro fluid dynamcis modes
It has the advantages such as easy to process, low cost, integrated level height, high flux, fluid flowing pulse free.Centrifugal force drives scope wide,
All can be driven on whole chip simultaneously, drive and realize simply, it is not necessary to extra pumping, it might even be possible to directly utilize existing
CD player.Microfluidic analysis technology passes through micro-processing technology by Functional Unit devices such as micro-valve, microchannel, micro-reative cell, Micropumps
It is integrated in as integrated circuit on chip material, coordinates optical detection, electrochemical measuring technique, may be implemented in cell, molecule
The detection of level, again because it is at material and the low consumption of reagent, in-situ study, the superiority such as the most real-time, at chemistry, biological field
Middle application is more and more extensive.
Centrifugal power-actuated micro-fluidic chip needs to realize the mixing of different liquids certain concentration, carry out testing and
During feature measurement, existing solution or employing human configuration, time-consuming long efficiency is low；Buy and prepared solution, cost
High；Interrupt experiments after chip runs a step, runs next step, again to realize function above after sample-adding.
And use and manually throw in sample, operation complexity, it is readily incorporated error, cost is high.
Summary of the invention
The technical problem to be solved designs to overcome the deficiencies in the prior art.Can be uninterruptedly
In the case of experiment, in same micro-fluidic chip, realize n (n >=1) plant the quantitative mixing of liquid, and by detection equipment pair
The method that mixture carries out detecting.
The present invention solves above-mentioned technical problem by the following technical solutions:
The one provided according to the present invention realizes the quantitatively mixing of n (n >=1) kind liquid in same micro-fluidic chip
Micro-fluidic chip, including chip body, helical duct, sample room, waste liquid chamber, measuring room, micro-valve, microchannel, mixed liquid chamber；In order to
It is easy to describe, using same from the radius of chip body center points toward edge as reference, by relatively close chip body center
Position is referred to as nearly heart side, and the position being relatively distant from chip body center is referred to as telecentricity side, described chip body arranges n bar spiral and leads to
Road, n is the integer more than or equal to 1, and described n bar helical duct is the most spaced apart on chip body, and every helical duct is from leaning on
Nearly chip body center initiates, and is spirally extended to terminate near chip body marginal position, and initiating terminal is provided with sample room, terminates
End is provided with waste liquid chamber, has measuring room along helical duct outer rim interval layout, and measuring room nearly heart side communicates with helical duct, meter
Amount telecentricity side, room connects has micro-valve, micro-valve to be opened on the helical duct of its telecentricity side, the measuring room of outermost helical duct remote
Heart side sets gradually micro-valve and microchannel, and microchannel telecentricity side arranges mixed liquid chamber.
By to sample room, helical duct, measuring room, micro-valve, the design of mixed liquid chamber and waste liquid pool etc., adjusting means is subject to
Centrifugal force size make sample in sample room enter corresponding helical duct, and by the speed size to rotatable platform
Regulation controls the flow regime of the fluid sample on micro-fluidic chip, makes sample under centrifugal action, enters and be full of meter
Amount room, surplus liquid flows into waste liquid pool, then under the micro-valve arrangement of capillary tube controls, with " the measuring room from outer layer helical duct
Measuring room to internal layer helical duct " order, sequentially enter mixed liquid chamber.By the size design to measuring room, control to enter
The volume of mixed liquid chamber；By the design to micro-valve, it is achieved sample enters the order of mixed liquid chamber.So, can be by different for concentration molten
Liquid is integrated on chip, it is achieved the purpose that the sample of multiple parallel reaction channel is thrown in automatically and certain concentration controls.Simple in construction,
Easy to operate.
The n bar helical duct arranged on micro-fluidic chip, n is alternatively the integer more than or equal to 2.And the bar number of helical duct
N, till maximum can increase to be limited can not be further added by because of the volume of described micro flow chip.
As the scheme optimized further of the micro-fluidic chip of the present invention, helical duct, sample room, waste liquid chamber can set
Put passage.
On the micro-fluidic chip that Ben Mingfa provides, micro-valve of the helical duct that ecto-entad is different, can be by its valve
The design of value, reaches to make fluid order on demand move in amount liquid chamber or the mixed liquid chamber in outside.As: can be designed to each
The resistance of micro-valve is from each micro-valve of telecentricity laterally nearly heart side, and its threshold value is gradually increased, during so that the micro flow chip speed of rotation becomes big,
The micro-valve realizing distal end is opened prior to the micro-valve of proximal part, during so that the micro flow chip speed of rotation becomes big, it is achieved nearly heart side amount liquid
Liquid in room sequentially enters in the amount liquid chamber of telecentricity side helical duct.
By to amount liquid chamber volume be arranged as required to, it is possible to achieve different liquids in mixed liquid chamber with need ratio
Mixing.
Although the accompanying drawing of the micro-fluidic chip that the present invention provides is circle, but the shape of chip can design as required
Become as on all rotatable platforms such as square, ellipse, rectangle, triangle, acceptable geometry (includes asymmetric
Geometric figure).
Need to realize the mixing of n kind liquid quantitative, and when mixture being detected by detection equipment, can be by following step
Rapid operation:
(1) in there is the sample room of micro-fluidic chip of n bar helical duct, it is separately added into liquid to be mixed；
(2) being placed on rotation platform by described micro flow chip, make micro-fluidic chip rotate, liquid enters helical duct,
And fill the multiple measuring rooms being positioned at helical duct telecentricity side successively, surplus liquid enters the waste liquid being positioned at helical duct clearing end
Room；
(3) gradually accelerate the speed of rotation of micro-fluidic chip, make liquid in measuring room with " telecentricity side helical duct
At first, the liquid in the measuring room of nearest heart side helical duct is last for liquid in measuring room " the order of ecto-entad, successively
Entering mixed liquid chamber, surplus liquid enters in waste liquid chamber；
(6) each liquid completes mixing and reaction in mixed liquid chamber, uses detection equipment to examine the liquid in mixed liquid chamber
Survey.
The present invention uses above technical scheme compared with prior art, has following technical effect that the present invention is based on turning
Micro flow control chip device on moving platform, by changing the velocity of rotation of rotatable platform, controls fluid sample in helical duct
Flow regime, control liquid and enter and be full of measuring room, and by regulate the opening and closing of micro-valve control fluid sample from outer to
Inside sequentially enter mixed liquid chamber.By the dimensional structure of measuring room and micro-valve is designed, it is achieved automatically throwing in and dense of several samples
The degree solution in step variation.The concrete size of counter structure can be entered according to the difference of application experiment object, operation sample
Row regulation, to adapt to different types of experimental implementation.Present invention achieves multiple parallel reaction channel sample automatically to throw in and specific
The control of concentration, and the mixed liquid chamber at the solution place of variable concentrations is distributed on one piece of micro-fluidic chip, available detection dress
Put and detect simultaneously.Simple in construction and easy to operate.
The track of helical duct, can be obtained by multiple equation of locus, such as, and track (X, the Y) parameter of helical duct
Equation can be:
Xt=r+R*t/2* π * cos (t) Yt=r+R*t/2* π * sin (t)
T is the radian between this locus of points and X-axis, and r is the distance of helical duct starting point and chip center, and (r+R) is
Helical duct terminating point and the distance of chip center.
Accompanying drawing explanation
Fig. 1 is bar number " n " value of helical duct on micro-fluidic chip in present invention when being 1, the schematic diagram of micro-fluidic chip.
Fig. 2 is bar number " n " value of helical duct on micro-fluidic chip in present invention when being 1, the top view of micro-fluidic chip.
Fig. 3 is bar number " n " value of helical duct on micro-fluidic chip in present invention when being 2, the schematic diagram of micro-fluidic chip.
Fig. 4 is bar number " n " value of helical duct on micro-fluidic chip in present invention when being 3, the schematic diagram of micro-fluidic chip.
Fig. 5 is bar number " n " value of helical duct on micro-fluidic chip in present invention when being 4, the schematic diagram of micro-fluidic chip.
Fig. 6 is bar number " n " value of helical duct on micro-fluidic chip in present invention when being 4, and micro-fluidic chip relates to office
Portion's helical duct, amount liquid chamber, micro-valve, microchannel, the local partial enlarged drawing of mixed liquid chamber.
Reference: 1-chip body, 2-sample room, 3-measuring room, 4-helical duct, the micro-valve of 5-, 6-microchannel, 7-mixes
Liquid chamber, 8-waste liquid chamber, 9-motor, 10-passage, 11-main shaft.
Detailed description of the invention
Below in conjunction with the accompanying drawings technical scheme is described further:
As shown in Figure 1 and Figure 2, it is a kind of micro-fluidic chip of the present invention, chip body arranges 1 helical duct, and accordingly
Sample room, helical duct, waste liquid chamber, measuring room, micro-valve, microchannel and mixed liquid chamber.Sample room is located close to chip center
Position, waste liquid chamber is located close to chip edge position, and described helical duct has proximal part and distal end, proximal part and sample
Room communicates, and distal end communicates with waste liquid pool, and multiple measuring rooms are distributed in outside helical duct along helical duct, and leads to spiral
Road communicates, and connecting outside each measuring room has a micro-valve；Microchannel is opened on the mixed liquid chamber of correspondence respectively.
Liquid joining sample room, uses slewing to make chip rotate, sample moves along helical duct, enters metering
In room, regulate chip rotary speed, make in samples room, regulate chip slewing rate so that sample enters helical duct, so
After under the influence of centrifugal force, sample respectively enters in 10 measuring rooms, and now the micro-valve of capillary tube is closed, sample without
Method flows out from measuring room, therefore can fill full measuring room moment, and redundant sample enters in waste liquid chamber；Increase chip rotating speed, make sample
Product respectively enter 10 mixed liquid chambers.Pre-setting the resistance of micro-valve, and the size of measuring room, micro-valve controls sample and enters mixed liquid
The order of room, the size Control sample of measuring room enters the volume of mixed liquid chamber, can realize the proportioning of sample room different proportion.
Due in chip structure can trapped air, when helical duct internal diameter is the least, passage need to be designed, passage position and
Quantity can be adjusted according to concrete application experiment scene.
Shown in Fig. 3, it is a kind of micro-fluidic chip of the present invention, chip body arranges 2 helical ducts, and corresponding sample
Room, helical duct, waste liquid chamber, measuring room, micro-valve, microchannel and mixed liquid chamber.Automatically throwing in and specific of 2 kinds of samples can be realized
The control of concentration.Specific operation process is as follows, is initially charged in 2 sample rooms two kinds of samples (sample A, sample B) (in order to describe
Convenient, here the helical duct adding sample A is referred to as helical duct A, the helical duct adding sample B is referred to as helical duct B), adjust
Save the speed that micro-hydraulic control chip rotates so that sample A and sample B respectively enters in the helical duct of correspondence, then at centrifugal force
Effect under, two kinds of samples respectively enter in the measuring room outside corresponding helical duct, now, the micro-valve of capillary tube of helical duct
Being closed, sample cannot flow out from measuring room, therefore can fill full measuring room moment, and redundant sample enters waste liquid chamber
In.Micro-valve of outer helical passage is farther away from chip center compared to micro-valve of inner side helical duct, therefore suffered centrifugal force is bigger,
Can open prior to micro-valve of inner side helical duct.Increase the rotating speed of micro flow chip so that micro-valve of outer helical passage is in be beaten
Open state, micro-valve of inner side helical duct is closed, then in the measuring room of outer helical passage, sample can pass through micro-valve
Mixed liquid chamber is entered through microchannel；Continuing to increase motor speed so that micro-valve of inner side helical duct is opened, inner side spiral leads to
Sample in the measuring room in road can pass through micro-valve, and under the influence of centrifugal force, moment by the measuring room of outer helical passage,
Micro-valve, microchannel, enter mixed liquid chamber.In the process, micro-valve controls the order of the mixed liquid chamber of sample entrance, the size of measuring room
Control sample and enter the volume of mixed liquid chamber, the proportioning of sample room different proportion can be realized.
2 helical ducts are set on table 1 micro-fluidic chip body, to realize 10 kinds of proportionings of 2 kinds of liquid
| The volume (microlitre) of upper 10 measuring rooms of helical duct A | 6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 | 5 |
| The volume (microlitre) of upper 10 measuring rooms of helical duct B | 15 | 13 | 11 | 17 | 19 | 16 | 11 | 13 | 14 | 8 |
| A: B (volume ratio) of mixed liquor in mixed liquid chamber | 6/15 | 7/13 | 8/11 | 9/17 | 10/19 | 1/6 | 2/11 | 3/13 | 4/14 | 5/8 |
Additionally, other operation schemes can be taked, as liquid can being placed in sample room simultaneously, by changing the knot of sample room
Structure and the contact angle with helical duct, it is achieved friction speed selects the sample in different sample room to enter the purpose of helical duct.
All the time acting on of the centrifugal force being subject in rotation process due to liquid so that only need to according to applicable liquid kind and demand not
The same regulation carrying out speed, measuring room size, micro-valve size and the design of structure channel size, measuring room and mixed liquid chamber quantity
Adjustment, just can realize two kinds of samples automatically throw in and solution concentration is stepped change.
Fig. 4 is a kind of micro-fluidic chip of the present invention, and chip body arranges 3 helical ducts, and corresponding sample room, spiral shell
Rotation passage, waste liquid chamber, measuring room, micro-valve, microchannel and mixed liquid chamber.Automatically throwing in and certain concentration of 3 kinds of samples can be realized
Control.Specific operation process is as follows, first puts in 3 sample rooms two kinds of samples (sample A, sample B, sample C) (in order to retouch
Stating conveniently, here the helical duct adding sample A is referred to as helical duct A, the helical duct adding sample B is referred to as helical duct B, adds
The helical duct of sample C is referred to as helical duct C).Regulate the speed that micro-hydraulic control chip rotates so that sample A, sample B and sample C
Respectively enteing in the helical duct of correspondence, the most under the influence of centrifugal force, 3 kinds of samples respectively enter outside corresponding helical duct
Measuring room in, now, the micro-valve of capillary tube of helical duct is closed, and sample cannot flow out from measuring room, therefore meeting
Moment fills full measuring room, and redundant sample enters in waste liquid chamber.Compared to inside it, each spiral leads to micro-valve of outer helical passage
Micro-valve in road is farther away from chip center, therefore suffered centrifugal force is bigger, can open prior to micro-valve of helical duct inside it.Increase micro-
The rotating speed of stream chip so that micro-valve of outermost helical duct is in open mode, inside it at micro-valve of all helical ducts
In closed mode, then in the measuring room of outermost helical duct, sample can enter mixed liquid chamber by micro-valve through microchannel；Continue
Continuous increase motor speed so that inside it, micro-valve of helical duct is opened successively, the sample in the measuring room of inner side helical duct
Meeting is by micro-valve, and under the influence of centrifugal force, moment passes through the measuring room of opposite exterior lateral sides helical duct, micro-valve, enters relatively
The measuring room of the helical duct in outside, and eventually pass through the mixed liquid chamber of microchannel entrance.In the process, micro-valve control sample enters
Entering the order of mixed liquid chamber, the size Control sample of measuring room enters the volume of mixed liquid chamber, can realize joining of sample room different proportion
Ratio.
3 helical ducts are set on table 2 micro-fluidic chip body, to realize 9 kinds of proportionings of 3 kinds of liquid
| The volume (microlitre) of upper 9 measuring rooms of helical duct A | 6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 |
| The volume (microlitre) of upper 9 measuring rooms of helical duct B | 8 | 10 | 9 | 13 | 15 | 7 | 9 | 8 | 13 |
| The volume (microlitre) of upper 9 measuring rooms of helical duct C | 11 | 14 | 12 | 13 | 17 | 7 | 11 | 13 | 17 |
| A: B: C (volume ratio) of mixed liquor in mixed liquid chamber | 6/8/11 | 7/10/14 | 8/9/12 | 9/13/13 | 10/15/17 | 1/7/7 | 2/9/11 | 3/8/13 | 4/13/17 |
Fig. 5 is a kind of micro-fluidic chip of the present invention, and chip body arranges 4 helical ducts, and corresponding sample room, spiral shell
Rotation passage, waste liquid chamber, measuring room, micro-valve, microchannel and mixed liquid chamber.Automatically throwing in and certain concentration of 4 kinds of samples can be realized
Control.Specific operation process is as follows, is first put in 4 sample rooms by two kinds of samples (sample A, sample B, sample C, sample D)
(for convenience, here the helical duct adding sample A being referred to as helical duct A, the helical duct adding sample B is referred to as spiral
Passage B, the helical duct adding sample C is referred to as helical duct C, and the helical duct adding sample D is referred to as helical duct D).Regulate micro-liquid
The speed that control chip rotates so that sample A, sample B, sample C and sample D respectively enter in the helical duct of correspondence, then exist
Under the effect of centrifugal force, 4 kinds of samples respectively enter in the measuring room outside corresponding helical duct, now, and the capillary of helical duct
Managing micro-valve to be closed, sample cannot flow out from measuring room, therefore can fill full measuring room moment, and redundant sample enters useless
In liquid chamber.Micro-valve of micro-valve of opposite exterior lateral sides helical duct each helical duct compared to inside it is farther away from chip center, therefore institute
Bigger by centrifugal force, can open prior to micro-valve of helical duct inside it.Increase the rotating speed of micro flow chip so that outermost spiral
Micro-valve of passage is in open mode, and inside it, micro-valve of all helical ducts is closed, then outermost helical duct
Measuring room in sample can enter mixed liquid chamber by micro-valve through microchannel；Continue to increase motor speed so that spiral shell inside it
Micro-valve of rotation passage is opened successively, and the sample in the measuring room of inner side helical duct can pass sequentially through micro-valve, and at centrifugal force
Under effect, by the measuring room of opposite exterior lateral sides helical duct, micro-valve, enter the measuring room of the helical duct of opposite exterior lateral sides, and
Mixed liquid chamber is entered eventually through microchannel.In the process, micro-valve controls the order of the mixed liquid chamber of sample entrance, the size of measuring room
Control sample and enter the volume of mixed liquid chamber, the proportioning of sample room different proportion can be realized.
4 helical ducts are set on table 3 micro-fluidic chip body, to realize 9 kinds of proportionings of 4 kinds of liquid
Above content is to combine concrete preferred implementation further description made for the present invention, it is impossible to assert
Being embodied as of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of present inventive concept, it is also possible to make some simple deductions or replacement, all should be considered as belonging to the present invention's
Protection domain.
Claims (9)
1. a micro-fluidic chip, it is characterised in that: include chip body (1), helical duct (4), sample room (2), waste liquid chamber
(8), measuring room (3), micro-valve (5), microchannel (6), mixed liquid chamber (7)；With same from chip body (1) center points toward edge
The position at relatively close chip body (1) center, as reference, is referred to as nearly heart side by radius, is relatively distant from chip body (1) center
Position be referred to as telecentricity side, described chip body (1) arranging n bar helical duct, n is the integer more than or equal to 1, described n bar spiral shell
Rotation passage is the most spaced apart on chip body (1), and every helical duct (4) initiates near chip body (1) center, spiral shell
Rotation extends close to chip body (1) marginal position and terminates, and initiating terminal is provided with sample room (2), and clearing end is provided with waste liquid chamber
(8), having measuring room (3) along helical duct (4) outer rim interval layout, the nearly heart side of measuring room (3) communicates with helical duct (4),
Measuring room (3) telecentricity side connects the helical duct (4) having micro-valve (5), micro-valve (5) to be opened on its telecentricity side, and outermost spiral leads to
The telecentricity side of the measuring room (3) in road (4) sets gradually micro-valve (5) and microchannel (6), and microchannel (6) telecentricity side arranges mixed
Liquid chamber (7).
Micro-fluidic chip the most according to claim 1, it is characterised in that n bar helical duct (4) arranged on chip, n is
Integer more than or equal to 2.
Micro-fluidic chip the most according to claim 1 and 2, track (X, the Y) parametric equation of described helical duct is:
Xt=r+R*t/2* π * cos (t) Yt=r+R*t/2* π * sin (t)
T is the point on helical duct and the angle between the line of chip center and X-axis, and r is in helical duct starting point and chip
The distance of the heart, (r+R) is the distance of helical duct terminating point and chip center.
4. according to the micro-fluidic chip described in claim 1,2 or 3, it is characterised in that the bar number n of helical duct (4), maximum can
Increase to till the chip body because of described micro flow chip (1) limited and can not be further added by.
5. according to the micro-fluidic chip described in claim 1,2 or 3, it is characterised in that helical duct (4), sample room (2), useless
Passage (10) it is additionally provided with on liquid chamber (8).
6. according to the micro-fluidic chip described in claim 1,2 or 3, it is characterised in that by each micro-valve of telecentricity laterally nearly heart side
(5), its threshold value is gradually increased, during so that the micro flow chip speed of rotation becomes big, it is achieved micro-valve (5) of distal end is micro-prior to proximal part
Valve (5) is opened.
7. according to the micro-fluidic chip described in claim 1,2 or 3, it is characterised in that the volume of described measuring room (3) is according to need
Arrange, to realize different liquids in mixed liquid chamber (7) with the ratio mixing needed.
8. according to the micro-fluidic chip described in claim 1 to 7, it is characterised in that described chip body (1) is circular.
9. one kind utilizes the micro-fluidic chip as according to any one of claim 1 to 8 to realize automatically throwing in and fixed of n kind liquid
Amount mixing, and the method detected mixture by detection equipment, comprise the following steps:
(1) in there is the sample room (2) of micro-fluidic chip of n bar helical duct, it is separately added into liquid to be mixed；
(2) being placed on rotation platform by described micro flow chip, make micro-fluidic chip rotate, liquid enters helical duct (4), and
Filling the multiple measuring rooms (3) being positioned at helical duct (4) telecentricity side successively, surplus liquid enters and is positioned at helical duct (4) termination
The waste liquid chamber (8) of end；
(3) gradually accelerate the speed of rotation of micro-fluidic chip, make the liquid in measuring room (3) with " telecentricity side helical duct (4)
Measuring room (3) in liquid at first, the liquid in the measuring room (3) of nearest heart side helical duct (4) is last " ecto-entad
Order, sequentially enter mixed liquid chamber (7), surplus liquid enters in waste liquid chamber (8)；
(6) each liquid completes mixing and reaction in mixed liquid chamber (7), uses detection equipment to carry out the liquid in mixed liquid chamber (7)
Detection.
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