CN106174260A - A kind of black Bulbus Allii water extract purposes in promoting intestinal emptying and containing the food of black Bulbus Allii water extract and medicine - Google Patents
A kind of black Bulbus Allii water extract purposes in promoting intestinal emptying and containing the food of black Bulbus Allii water extract and medicine Download PDFInfo
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- CN106174260A CN106174260A CN201610541341.9A CN201610541341A CN106174260A CN 106174260 A CN106174260 A CN 106174260A CN 201610541341 A CN201610541341 A CN 201610541341A CN 106174260 A CN106174260 A CN 106174260A
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- bulbus allii
- black bulbus
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- water extract
- black
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- 239000000284 extract Substances 0.000 title claims abstract description 69
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 21
- 239000003814 drug Substances 0.000 title claims abstract description 16
- 235000013305 food Nutrition 0.000 title claims abstract description 16
- 230000001737 promoting effect Effects 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000012675 alcoholic extract Substances 0.000 claims abstract description 6
- 238000000622 liquid--liquid extraction Methods 0.000 claims abstract description 6
- 230000008569 process Effects 0.000 claims abstract description 6
- 238000000638 solvent extraction Methods 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims description 8
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 6
- 235000013824 polyphenols Nutrition 0.000 claims description 6
- 102000019197 Superoxide Dismutase Human genes 0.000 claims description 4
- 108010012715 Superoxide dismutase Proteins 0.000 claims description 4
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 claims description 3
- 210000002784 stomach Anatomy 0.000 description 21
- 210000000936 intestine Anatomy 0.000 description 19
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 18
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 12
- 238000000605 extraction Methods 0.000 description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- ZFAHNWWNDFHPOH-YFKPBYRVSA-N S-allylcysteine Chemical compound OC(=O)[C@@H](N)CSCC=C ZFAHNWWNDFHPOH-YFKPBYRVSA-N 0.000 description 8
- UBAXRAHSPKWNCX-UHFFFAOYSA-N diallyl trisulfide Chemical compound C=CCSSSCC=C UBAXRAHSPKWNCX-UHFFFAOYSA-N 0.000 description 6
- 230000001141 propulsive effect Effects 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- ZFAHNWWNDFHPOH-UHFFFAOYSA-N S-Allyl-L-cystein Natural products OC(=O)C(N)CSCC=C ZFAHNWWNDFHPOH-UHFFFAOYSA-N 0.000 description 4
- PFRGXCVKLLPLIP-UHFFFAOYSA-N diallyl disulfide Chemical compound C=CCSSCC=C PFRGXCVKLLPLIP-UHFFFAOYSA-N 0.000 description 4
- GJYVZUKSNFSLCL-UHFFFAOYSA-N dichloromethanol Chemical compound OC(Cl)Cl GJYVZUKSNFSLCL-UHFFFAOYSA-N 0.000 description 4
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- XUHLIQGRKRUKPH-GCXOYZPQSA-N Alliin Natural products N[C@H](C[S@@](=O)CC=C)C(O)=O XUHLIQGRKRUKPH-GCXOYZPQSA-N 0.000 description 3
- 240000002234 Allium sativum Species 0.000 description 3
- UBJVUCKUDDKUJF-UHFFFAOYSA-N Diallyl sulfide Chemical compound C=CCSCC=C UBJVUCKUDDKUJF-UHFFFAOYSA-N 0.000 description 3
- XUHLIQGRKRUKPH-UHFFFAOYSA-N S-allyl-L-cysteine sulfoxide Natural products OC(=O)C(N)CS(=O)CC=C XUHLIQGRKRUKPH-UHFFFAOYSA-N 0.000 description 3
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- XUHLIQGRKRUKPH-DYEAUMGKSA-N alliin Chemical compound OC(=O)[C@@H](N)C[S@@](=O)CC=C XUHLIQGRKRUKPH-DYEAUMGKSA-N 0.000 description 3
- 235000015295 alliin Nutrition 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002212 flavone derivatives Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 108010068906 gamma-glutamylcysteine Proteins 0.000 description 3
- 235000004611 garlic Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 3
- RITKHVBHSGLULN-CRCLSJGQSA-N γ-glutamylcysteine Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](CS)C(O)=O RITKHVBHSGLULN-CRCLSJGQSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 108010005094 Advanced Glycation End Products Proteins 0.000 description 1
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 230000010718 Oxidation Activity Effects 0.000 description 1
- 235000011158 Prunus mume Nutrition 0.000 description 1
- 244000018795 Prunus mume Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical class [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 description 1
- 235000010081 allicin Nutrition 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 210000002318 cardia Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- RMKCQUWJDRTEHE-UHFFFAOYSA-N diallyl tetrasulfane Natural products C=CCSSSSCC=C RMKCQUWJDRTEHE-UHFFFAOYSA-N 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- FGXWKSZFVQUSTL-UHFFFAOYSA-N domperidone Chemical compound C12=CC=CC=C2NC(=O)N1CCCN(CC1)CCC1N1C2=CC=C(Cl)C=C2NC1=O FGXWKSZFVQUSTL-UHFFFAOYSA-N 0.000 description 1
- 229960001253 domperidone Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- PYGSKMBEVAICCR-UHFFFAOYSA-N hexa-1,5-diene Chemical group C=CCCC=C PYGSKMBEVAICCR-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 230000035943 smell Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000005987 sulfurization reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention discloses a kind of black Bulbus Allii water extract purposes in promoting intestinal emptying, and contain food and the medicine of described black Bulbus Allii water extract, described black Bulbus Allii water extract after the alcoholic extract black Bulbus Allii of 50% to obtain the thick extract of black Bulbus Allii, the water layer obtained so that water carries out liquid-liquid extraction by thick for described black Bulbus Allii extract again, can make small intestinal Emptying Rate under process effective dose is 65% to 80%.
Description
[technical field]
The present invention relates to the purposes field of black Bulbus Allii extract, particularly relate to black Bulbus Allii water extract in promoting intestinal emptying
Purposes, and comprise medicine and the food of black Bulbus Allii water extract.
[background technology]
Since ancient times, Bulbus Allii is in diet and is widely used as food materials or flavouring, in addition to as food materials,
Also having good physiologically active, the especially medical material of " medicine-food two-purpose ", Traditional Chinese Medicine ancient books and records are recorded: Bulbus Allii acrid in the mouth, warm in nature, have
The effect of " warming middle-JIAO stomach invigorating, helps digestion and regulate the flow of vital energy, and detoxify parasite killing ".Research report also indicates that Bulbus Allii has good antioxidation, antibacterial, pre-
The physiologically actives such as anti-cardiovascular disease and good antitumor action.Additionally, research also indicates that Bulbus Allii also has good relieving constipation effect
Really.Bulbus Allii main active, from fat-soluble sulfide contained in Bulbus Allii, comprises alliin (alliin), garlicin
(allicin), diallyl sulfide (diallyl suifide, DAS), diallyl disulfide (diallyl
Disulfide, DADS) and diallyl trisulfide (diallyl trisulfide, DATS) etc., but, Bulbus Allii is through broken
Rear produce unique acid, essentially from garlicin, allow the stepping back of many prestiges.
The manufacture process of black Bulbus Allii be mainly control garbled fresh garlic to ferment in the environment of high temperature with high humidity ripe
Becoming, this reacts for Mei Na.Receiving in reaction procedure on prunus mume (sieb.) sieb.et zucc., the distinctive garlicin of Bulbus Allii is converted into without garlic odour, the sulfuration of low irritant
Thing, gamma-glutamyl cysteine (γ-glutamyl cysteine) changes generation S-allyl cysteine (S-allyl
Cysyeine, SAC), breaks down proteins becomes amino acid, carbohydrate breakdown to become fructose, and garlic clove is also changed into by original white
Pitchy, last cooled being dried forms.Black Bulbus Allii is soft and high resilience, after entrance soft rotten fragrant and sweet and pungent sense without Bulbus Allii and
Unhappy abnormal smells from the patient, direct-edible, for the health food that American-European countries in recent years, Japan and Korea S etc. are popular.Mei Na reaction is sent out
The raw amido being primarily due in food produces non-ferment browning reaction with carbonyl, it is considered that, the product of Mei Na reaction
(maillard reaction products, MRP) can give the special color and luster of food and local flavor, and research also indicates that, black Bulbus Allii system
Cheng Zhong, Mei Na reaction makes saccharide have the antioxidant activity more higher than Bulbus Allii with the MRP of amino acid.But the freshest few people
Inquire into other purposes of black Bulbus Allii extract further.
[summary of the invention]
Present stage, have no the research in terms of promoting the intestines and stomach emptying of the black Bulbus Allii extract and report, in consideration of it, the present invention proposes
A kind of black Bulbus Allii water extract purposes in promoting the intestines and stomach emptying, and black Bulbus Allii water extract applies in food and medicine.
The preparation method of described black Bulbus Allii water extract obtains the thick extract of black Bulbus Allii after the alcoholic extract black Bulbus Allii of 50%,
The water layer thicker for black Bulbus Allii extract obtained so that water carries out liquid-liquid extraction.
Described black Bulbus Allii water extract can make small intestinal Emptying Rate under process effective dose is at 200mg/KG～500mg/KG
Be 65% to 80%.
The Determination of Polyphenols of described black Bulbus Allii extract is 50mg/ml to 62mg/ml.
The superoxide dismutase activity of described black Bulbus Allii extract is 4000U/g to 5000U/g.
Described black Bulbus Allii extract comprises water-soluble sulfide.
Further, the present invention reoffers a kind of food with the intestines and stomach air emptying function, and it comprises black Bulbus Allii water extract.
Further, the present invention reoffers a kind of medicine with the intestines and stomach air emptying function, and it comprises black Bulbus Allii water extract.
The beneficial effects of the present invention is: in black Bulbus Allii manufacture process, Bulbus Allii, without any processing Rogue program, makes
When preparing food or medicine with black Bulbus Allii water-soluble extract, the most relatively relaxing the zest of the intestines and stomach, the intestines and stomach emptying effect levels off to
The medicine of general commercially available promotion the intestines and stomach emptying.
[accompanying drawing explanation]
The thick extract of Fig. 1, black Bulbus Allii is with 100mg black Bulbus Allii: 100ml normal hexane, dichloro methanol, ethyl acetate, n-butyl alcohol and
Water carries out being layered extraction procedure figure.
[detailed description of the invention]
The present invention proposes a kind of black Bulbus Allii water extract purposes in promoting the intestines and stomach emptying, and one has promotion the intestines and stomach
The food containing black Bulbus Allii water extract of air emptying function and medicine.
Slightly extract obtaining black Bulbus Allii after the alcoholic extract black Bulbus Allii that the preparation method of above-mentioned black Bulbus Allii water extract can use 50%
Thing, then black Bulbus Allii is slightly extracted thing carry out, with water, water layer that liquid-liquid extraction obtained and obtain, but be not limited, also can directly with
Water extraction black Bulbus Allii slightly extracts thing and obtains black Bulbus Allii water extraction thing.
Above mode can make the Determination of Polyphenols of black Bulbus Allii water extract between 50mg/ml to 62mg/ml, superoxides discrimination
Change enzymatic activity can be extracted out in water layer between 4000U/g to 5000U/g, the extract this means, possessing antioxidant activity.
Additionally, in black Bulbus Allii manufacture process, Bulbus Allii, without any processing Rogue program, is only induced by the regulation and control of temperature with humidity
Mei Na reacts, and therefore, the allinase in raw Bulbus Allii can't disengage cracking acid propylhomoserin and produce garlicin, or makes garlicin change
For alliin.But the gamma-glutamyl cysteine in Bulbus Allii is converted into more non-irritating water solublity sulfur-containing compound S-
Allyl cysteine (SAC).Therefore, when using black Bulbus Allii water-soluble extract to prepare food or medicine, the stimulation to the intestines and stomach
Property the most relatively relaxes.
In order to be more fully understood that technical scheme, it is described in detail further below by way of specific embodiment:
1, the extraction mode of black Bulbus Allii extract:
Weigh after black Bulbus Allii is smashed, use the ethanol of 50%, with black Bulbus Allii: the weight ratio of ethanol=1:4 extracts 3 times, to take
Obtain black Bulbus Allii and slightly extract thing.Afterwards, the normal hexane of the 5 times of weight slightly extracting thing with black Bulbus Allii carries out liquid-liquid extraction, filtration after having extracted, weight
This step multiple at least 3 times, after extraction completely, more sequentially with the dichloro methanol of 100mg black Bulbus Allii: 100ml, ethyl acetate, n-butyl alcohol
And water carry out liquid-liquid extraction according to abovementioned steps, extraction process coordinates analyzes its composition with GC or HPLC, to confirm to have extracted
Entirely, the extracting and demixing of 5 opposed polarities of normal hexane, dichloro methanol, ethyl acetate, n-butyl alcohol and water can finally be obtained.
2, Intestinal propulsive rate measures:
The gavage solution configured by rat fills food, and gavage solution contains the activated carbon of 10% weight ratio and black Bulbus Allii to be measured
Thick extract, then tracing computation Intestinal propulsive rate, using activated carbon is that the characteristic utilizing its color and easily observation is as the intestines and stomach
Another index of emptying.After 30 minutes, rat decaptitates execution, cuts off abdominal cavity subsequently and pinions at cardia with line, by complete to stomach, small intestinal
Whole taking-up, observes the dry weight of feces, grain number, moisture and shape, and calculates carbon transfer rate and represent Intestinal propulsive rate (the intestines and stomach empties
Rate), the formula of Intestinal propulsive rate is as follows:
Carbon transfer rate=(activated carbon labelling/intestinal length) x100%
Its result is as shown in Table 1:
The thick extract of table one, black Bulbus Allii extract thick with raw Bulbus Allii compares for the intestines and stomach emptying
The extraction mode of the thick extract of raw Bulbus Allii is identical with the extraction mode of the thick extract of black Bulbus Allii, weighs after being smashed by raw Bulbus Allii,
Use the ethanol of 50%, with raw Bulbus Allii: the weight ratio of ethanol=1:4 extracts 3 times, slightly extract thing obtaining raw Bulbus Allii.
Table one be comparative drug DMP (many Pulis are many, Domperidone) (Taiwan Yongxin Medicines and Chemical Reagents Co., Ltd.,
Licence font size 032358, health care AC32358100), the thick extract of black Bulbus Allii of 50% alcoholic extract, 50% alcoholic extract
Raw Bulbus Allii slightly takes extraction thing, the Intestinal propulsive rate (the intestines and stomach Emptying Rate) under treatment dosage is 500mg/kg.As shown in Table 1, feeding is black
The thick extract of Bulbus Allii can effectively facilitate the intestines and stomach emptying, and its Intestinal propulsive rate reaches 62 ± 8.2%, is better than the thick extract of raw Bulbus Allii, close to DMP
The intestines and stomach Emptying Rate.
As shown in Fig. 1 extraction procedure figure, by thick for black Bulbus Allii extract the most sequentially with 100mg black Bulbus Allii: 100ml normal hexane,
Dichloro methanol, ethyl acetate, n-butyl alcohol and water carry out layering extraction, during using total extraction quantity as 100%, each part layering
Ratio is as shown in Table 2:
Table two
As shown in Table 2, after difference layering extraction, n-butanol layer and water layer have higher extraction yield, therefore select this
Two-layer carries out oxidation resistance analysis discussion.
Use conventional polyphenol, flavone and superoxide dismutase method of testing to n-butanol layer, water layer extract, and
The thick extract of raw Bulbus Allii carries out polyphenol, flavone and superoxide dismutase activity assay, result as shown in Table 3:
Table three
The result display black Bulbus Allii water layer extract SOD activity of table three is 4460.43 ± 329.68U/g, and Determination of Polyphenols is
59.52 ± 0.18mg/mL, general flavone content is then that n-butanol layer content is higher, represents that water layer extract possesses bigger resisting
Oxidation activity potentiality, therefore select water layer extract carry out the intestines and stomach depletion test, result as shown in Table 4:
Table four
Can be learnt by table four, when dosage is 500mg/kg, black Bulbus Allii water layer extract Emptying Rate up to 69.3 ± 8.1%,
Leveling off to and usually promote the medicine DMP of the intestines and stomach emptying, therefore black Bulbus Allii water layer extract has the effect promoting the intestines and stomach emptying really.
The foregoing is only illustrative, rather than be restricted person.Any spirit and scope without departing from the present invention, and to it
The equivalent modifications carried out or change, in claim attached after being intended to be limited solely by.
Claims (6)
1. the black Bulbus Allii water extract purposes in promoting intestinal emptying, it is characterised in that described black Bulbus Allii water extract with
To obtain the thick extract of black Bulbus Allii after the alcoholic extract black Bulbus Allii of 50%, thicker for described black Bulbus Allii extract is carried out liquid-liquid extraction with water
And the water layer obtained, described black Bulbus Allii water extract can make small intestinal arrange under process effective dose is at 200mg/KG ~ 500mg/KG
Empty rate is 65% to 80%.
2. the black Bulbus Allii water extract as claimed in claim 1 purposes in promoting intestinal emptying, it is characterised in that described is black
The Determination of Polyphenols of Bulbus Allii water extract is 50 mg/ml to 62 mg/ml.
3. the black Bulbus Allii water extract as claimed in claim 1 purposes in promoting intestinal emptying, it is characterised in that described is black
The superoxide dismutase activity of Bulbus Allii water extract is 4000 U/g to 5000 U/g.
4. the black Bulbus Allii water extract as claimed in claim 1 purposes in promoting intestinal emptying, it is characterised in that described is black
Bulbus Allii water extract comprises water-soluble sulfide.
5. one kind contains the food just like the black Bulbus Allii water extract described in claim 1,2,3 or 4, it is characterised in that described food
It is applied to promote the purposes of intestinal emptying.
6. one kind contains the medicine just like the black Bulbus Allii water extract described in claim 1,2,3 or 4, it is characterised in that described medicine
It is applied to promote the purposes of intestinal emptying.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610541341.9A CN106174260B (en) | 2016-07-11 | 2016-07-11 | Application of black garlic water extract in promoting intestinal tract to drain air, and food and medicine containing black garlic water extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610541341.9A CN106174260B (en) | 2016-07-11 | 2016-07-11 | Application of black garlic water extract in promoting intestinal tract to drain air, and food and medicine containing black garlic water extract |
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| CN106174260A true CN106174260A (en) | 2016-12-07 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107334913A (en) * | 2016-12-12 | 2017-11-10 | 育生（厦门）生物科技有限公司 | Black garlic extract and preparation method thereof and purposes |
| CN107647276A (en) * | 2017-08-31 | 2018-02-02 | 天津科技大学 | A kind of black garlic slurry stone mill vermicelli and preparation method thereof |
| TWI654990B (en) * | 2016-12-12 | 2019-04-01 | 大陸商育生（廈門）生物科技有限公司 | Preparing method of black garlic extracts |
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| CN107334913A (en) * | 2016-12-12 | 2017-11-10 | 育生（厦门）生物科技有限公司 | Black garlic extract and preparation method thereof and purposes |
| TWI654990B (en) * | 2016-12-12 | 2019-04-01 | 大陸商育生（廈門）生物科技有限公司 | Preparing method of black garlic extracts |
| CN107334913B (en) * | 2016-12-12 | 2021-04-30 | 育生（厦门）生物科技有限公司 | Black garlic extract and preparation method and application thereof |
| CN107647276A (en) * | 2017-08-31 | 2018-02-02 | 天津科技大学 | A kind of black garlic slurry stone mill vermicelli and preparation method thereof |
Also Published As
| Publication number | Publication date |
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| CN106174260B (en) | 2021-08-17 |
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