CN106171890A - A kind of method of intermittent warming Bulbus Lilii son's ball phase transformation - Google Patents
A kind of method of intermittent warming Bulbus Lilii son's ball phase transformation Download PDFInfo
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- CN106171890A CN106171890A CN201610570494.6A CN201610570494A CN106171890A CN 106171890 A CN106171890 A CN 106171890A CN 201610570494 A CN201610570494 A CN 201610570494A CN 106171890 A CN106171890 A CN 106171890A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
A kind of method that the invention provides intermittent warming Bulbus Lilii son's ball phase transformation, described method is: OT Bulbus Lilii " Robina " scale taking cuttage at 25 DEG C obtains the little young ball of Bulbus Lilii after 20 weeks, little young ball is selected and carried out intermittent warming, considerably increases lily ball and breed cost.By research Bulbus Lilii son's ball phase conversion mechanism, shortening Bulbus Lilii juvenile stage, thus shorten the Lilies breeding time limit, reduction Bulbus Lilii breeds cost and reaches lily ball industrialization.
Description
Technical field
A kind of method that the present invention relates to intermittent warming Bulbus Lilii son's ball phase transformation, belongs to breeding and cultivation field, gardens.
Background technology
Bulbus Lilii is Liliaceae lilium perennial herb flowering bulb.Commonly use and do flower culture, be also world-renowned five
One of big Fresh Cutting flower.Bulbus Lilii is in addition to common Lilium Pollyanna, oriental hybrid lily, lilium longiflorum hybrid system, and LA, OT, OA etc. are novel
Hybrid system kind gradually come out (Liu little Xi etc., 2011.；Wu Longyun etc., 2013.；Ge Liang, 2012 etc.).OT strain Bulbus Lilii excellent
Point has cane thick, scape just, the features such as petal plumpness is beautiful, and planting time is short, and disease resistance is strong, long fresh-keeping period so that international city
The demand of OT Bulbus Lilii is incrementally increased by field.Since OT Bulbus Lilii introduces China, the most well received, its cultivated area constantly expands
Greatly, development 0T Bulbus Lilii be following Bulbus Lilii industry development priority (woods Xiao Hong etc., 2013；Week is thick high, and 2004).
The domestic substantial amounts of fresh cut lily flowers of annual consumption, some scale Bulbus Lilii flower shows also consume a large amount of lily ball.But
These lily balls mostly are import, it is achieved lily ball industrialization is always the dream of Bulbus Lilii researcher.Lily ball produces
There are many important technological problems to need to solve during industry: virus-free, shorten kind of ball incubation time thus reduce kind of a ball and train into
This, strengthen kind of ball resistance (the most degeneration-resistant saline and alkaline and drought stress) etc..
Bulbus Lilii grows and is divided into three phases: juvenile growth phase, grow up trophophase, reproductive stage.The transformation of three phases
It is called phase transformation.The little young ball First Year of Bulbus Lilii after cuttage only grows 1 leaf, and photosynthesis is few；Nature needs through 1-2
The season of growth (1-2) just can be trained the young ball of stem；After growing stem, photosynthesis is strengthened, organic matter accumulation is accelerated,
Lily ball expands could be accelerated.Bulbus Lilii majority kind happiness subacidity soil, soils of north China meta-alkali, the season of growth is to Bulbus Lilii root system
Causing lasting Saline Alkali Stress, this had both affected kind of a ball speed of growth, again impact kind ball quality.Coerce and may cause plant membrane system
Peroxidating；By improving oxidation resistance, the anti-adversity ability of plant can be improved.Studied by relevant phase transition temperature, for Bulbus Lilii
Plant ball degeneration-resistant molecular breeding and important foundation is provided.Thus improve lily cultivar resistance, reach lily ball industrialization.
Summary of the invention
It is an object of the invention to provide the present invention provides a kind of parks' water system water circulation to purify technique.
In order to achieve the above object, the present invention provides following technical scheme:
A kind of method of intermittent warming Bulbus Lilii son's ball phase transformation, it is characterised in that: take healthy OT Bulbus Lilii Robina kind ball, with squama
Stem for according to be divided into outer, in, interior different levels carry out classification cuttage；General planting is carried out after being divided into three to process；Room temperature 25 DEG C
Process 20 weeks, follow-up intermittent warming, breaking dormancy.
Further, at the peat composed of rotten mosses: Vermiculitum: perlite is in the substrate of 2:1:1, cuttage outer layer, middle level, internal layer squama respectively
Three layers of scale, after 25 DEG C of cuttages 20 weeks, are randomly selected 300 pieces by sheet, add up the young ball weight formed on each layer scale, classification
Standard is as follows: 800～900mg, 700～800mg, 600～700mg, 500～600mg, 400～500mg, 300～400mg,
200～300mg, 100～200mg.Add up the quantity generating young ball on each heavyweight each layer scale.
Further, select the outer scale of Bulbus Lilii " Robina " kind after under the conditions of 25 DEG C cuttage 20 weeks, select
The little young ball of weight Bulbus Lilii in the range of 500-600mg, carries out the process of temperature and time during intermittent warming to little young ball,
Then breaking dormancy K cryogenic treatment 10 weeks under the conditions of 4 DEG C.
Further, Bulbus Lilii outer scale is carried out cuttage, extend cutting time, adjust room temperature and process the time, select weight
Measure the young ball in 500-600mg scope, young ball is carried out 4 weeks intermittent warming of 15 DEG C, then it is carried out 4 DEG C of K cryogenic treatment 10
Week, breaking dormancy.
Further, select Bulbus Lilii high-quality kind ball to outer layer, middle level, the classification cuttage of internal layer scale, skewer under the conditions of 25 DEG C
Inserting 20 weeks, the young ball forming scale cuttage carries out weight grading, and grade scale is as follows: 800～900mg, 700～800mg,
600～700mg, 500～600mg, 400～500mg, 300～400mg, 200～300mg, 100～200mg, 8 grades；So
After all young balls are preserved 4 weeks under the conditions of 15 DEG C, K cryogenic treatment 10 weeks under the conditions of 4 DEG C.After in basket general planting cultivate.
The present invention, in the research of young ball intermittent warming induced transformation, takes OT Bulbus Lilii " Robina " scale of cuttage at 25 DEG C
Obtain the little young ball of Bulbus Lilii after 20 weeks, little young ball is selected and carried out intermittent warming, finds after process: (1) outer bulb, middle bulb skewer
Inserting the little young ball grown all to exist 100～900mg, interior bulb fails to grow the young ball of 700～900mg, it may be possible to by
Insufficient in the nutrient accumulation of interior bulb, fail to provide sufficient nutrition to little young ball.Scale formation 600～the energy of 900mg son's ball
Power is from high to low: outer scale ＞ middle level scale ＞ internal layer scale；Scale formed 300～600mg son's ball ability by height to
The end is: middle level scale ＞ outer scale ＞ internal layer scale；Scale forms 100～300mg.(2) in the examination of time-triggered protocol that relents
In testing, the Bulbus Lilii son's ball through the intermittent warming of 15 DEG C is more vulnerable to certain than Bulbus Lilii son's ball of 11 DEG C, 19 DEG C intermittent warming and lures
Lead, improve trunk diameter growth rate, produce phase transformation.(3), during room temperature processes the research of induced transformation, the lily bulb of cuttage is through 20
The room temperature process in week 25 DEG C, 4 weeks 15 DEG C of intermittent warming and the generation of 10 weeks K cryogenic treatment induced transformations preferably of 4 DEG C.(4) each
On level scale, different young ball sizes are in the impact of young ball phase transformation, find that the young ball that young ball specific mass that quality is big is little is more easy to produce
Raw phase transformation.
Studied by relevant phase transition temperature, provide important foundation for the degeneration-resistant molecular breeding of lily ball.Thus improve hundred
Close varietal resistance, reach lily ball industrialization.Considerably increase lily ball and breed cost.By research Bulbus Lilii son's ball phase transformation
Mechanism, shortens Bulbus Lilii juvenile stage, thus shortens the Lilies breeding time limit, and reduction Bulbus Lilii breeds cost and reaches lily ball industrialization.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to
Other accompanying drawing is obtained according to these accompanying drawings.
Fig. 1 difference scale cuttage forms young ball capacity of water；
Fig. 2 difference inducing temperature and time bearing ball growth stem statistics；
Fig. 3 extends cutting time to young ball phase transformation impact；
Fig. 4 difference cuttage position Bulbus Lilii son's ball growth stem rate.
Detailed description of the invention
Technical scheme is clearly and completely described by the accompanying drawing below in conjunction with the present invention, it is clear that retouched
The embodiment stated is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, this
The every other embodiment that field those of ordinary skill is obtained under not making creative work premise, broadly falls into the present invention
The scope of protection.
Embodiment 1
1.1 test material
Take healthy OT Bulbus Lilii Robina kind ball, with bulb for according to be divided into outer, in, interior different levels carry out classification cuttage.
General planting is carried out after being divided into three to process.Room temperature 25 DEG C processes 20 weeks, follow-up intermittent warming, breaking dormancy.According to experiment need
Carry out selecting and processing respectively.
1.1.1 testing equipment equipment
1) Medical refrigerator YC-260 (U.S. of middle section Pedicellus et Pericarpium Trapae)
2) intelligence illumination box (Ningbo Hai Shusaifu experimental apparatus factory)
1.2 EXPERIMENTAL DESIGN
1.2.1 different cuttage positions form young ball size ability
At the peat composed of rotten mosses: Vermiculitum: perlite is in the substrate of 2:1:1, cuttage outer layer, middle level, internal layer scale respectively, at 25 DEG C of skewers
After inserting 20 weeks, three layers of scale being randomly selected 300 pieces, add up the young ball weight formed on each layer scale, grade scale is as follows:
800～900mg, 700～800mg, 600～700mg, 500～600mg, 400～500mg, 300～400mg, 200～300mg,
100～200mg.Add up the quantity generating young ball on each heavyweight each layer scale.
1.2.2 young ball inducing temperature and the time impact on young ball phase transformation
Select the outer scale of Bulbus Lilii " Robina " kind after under the conditions of 25 DEG C cuttage 20 weeks, select weight at 500-
The little young ball of Bulbus Lilii in the range of 600mg, carries out the process of temperature and time during intermittent warming, then at 4 DEG C to little young ball
Under the conditions of breaking dormancy K cryogenic treatment 10 weeks.Young ball inducing temperature and the processing method of time are shown in Table 2-1.
Table 2-1 son's bulb temperature and the process of time
Table.2-1The induction temperature and time to deal with bulblets
1.2.3 the cutting time impact on young ball phase transformation is extended
Bulbus Lilii outer scale is carried out cuttage, extends cutting time, adjust room temperature and process the time, select weight at 500-
The young ball of 600mg scope, carries out 4 weeks intermittent warming of 15 DEG C to young ball, then it is carried out 4 DEG C of K cryogenic treatment 10 weeks, break not
Sleep." Robina " extends the processing method of cutting time and is shown in Table 2-2.
Table 2-2 extends cutting time and processes
Table.2-2Extend the time of cutting processing of bulblets
The different young ball sizes impact on young ball phase transformation on the most each level scale
Selecting Bulbus Lilii high-quality kind ball to outer layer, middle level, the classification cuttage of internal layer scale, under the conditions of 25 DEG C, cuttage 20 weeks, right
The young ball that scale cuttage is formed carries out weight grading, and grade scale is as follows: 800～900mg, 700～800mg, 600～700mg,
500～600mg, 400～500mg, 300～400mg, 200～300mg, 100～200mg, 8 grades.Then by all young balls
Preserve 4 weeks under the conditions of 15 DEG C, K cryogenic treatment 10 weeks under the conditions of 4 DEG C.After in basket general planting cultivate.
1.3 results and analysis
1.3.1 different scale cuttages form young ball size ability
As seen from Figure 1, the little young ball that outer bulb, middle bulb cuttage grow all existed in each stage, and interior bulb is not
The young ball of 700～900 can be grown, it may be possible to owing to the nutrient accumulation of interior bulb is insufficient, fail to provide abundance to little young ball
Nutrition.The ability of scale formation 600～900mg son's ball is from high to low: outer scale ＞ middle level scale ＞ internal layer scale；Scale
The ability of formation 300～600mg son's ball by height is on earth: middle level scale ＞ outer scale ＞ internal layer scale；Scale formed 100～
The ability of 300mg son's ball is from high to low: internal layer scale ＞ middle level scale ＞ outer scale.
1.3.2 inducing temperature and the time impact on young ball phase transformation
Such as Fig. 2 it can be seen that in intermittent warming, temperature T5, T2, T6 are respectively alternating temperature 11 DEG C, 15 DEG C and 19 DEG C, it is main
Stem nematode rate trunk diameter growth rate when 15 DEG C is the highest, optimum trunk diameter growth.In whether intermittent warming, 15 DEG C of changes in T1, T2
The T2 trunk diameter growth rate that temperature processes is approximately 2 times without intermittent warming T1.Illustrate that Bulbus Lilii son's ball needs intermittent warming.When alternating temperature
Between process in, T3, T2, T4, T3 be 15 DEG C process 2 weeks, T2 be 15 DEG C process 4 weeks, T4 be 15 DEG C process 6 weeks, result is stem
Downward trend after taking the lead in rising, at T2, during 15 DEG C process 4 weeks, growth stem is the highest.
1.3.3 cutting time is extended on young ball phase transformation impact
Such as Fig. 3, in room temperature induction produces the research of phase transformation, lily bulb during the room temperature cuttage of 25 DEG C, along with
The change of room temperature time, Bulbus Lilii son's ball trunk diameter growth rate be E1 be 92.875%, E2 be 75%, E3 be 86.9565%.Stem is raw
Long rate is E1 ＞ E3 ＞ E2, illustrates that the room temperature of 20 weeks processes i.e. available higher trunk diameter growth rate, 22 weeks, at the room temperature of 24 weeks
Reason will not increase Bulbus Lilii son's ball growth stem rate, and along with the room temperature time changes, stem rate rises after falling before.In E1:20 week 25
Bulbus Lilii son's ball, i.e. available higher growth stem rate is induced to promote Bulbus Lilii son's ball phase under conditions of DEG C+4 weeks in week 15 DEG C+10 4 DEG C
Become.
On the most each level scale, different young ball sizes are on young ball phase transformation impact
Such as Fig. 4, in the ability that Bulbus Lilii son's ball quality is 600～900mg growth stems it is from high to low: outside the scale ＞ of middle level
Layer scale ＞ internal layer scale；300～600mg son's ball growth stem ability by height be on earth: internal layer scale ＞ outer scale ＞
Middle level scale；The internal layer scale of the young ball of 100～300mg, middle level scale, outer scale all fail to grow stem.Bulbus Lilii is described
Young ball quality is the major reason of Bulbus Lilii son's ball growth stem, and Bulbus Lilii son's ball quality is the biggest, and the probability of growth stem is the biggest.
1.4 conclusions and discussion
In the research of young ball intermittent warming induced transformation, take OT Bulbus Lilii " Robina " scale of cuttage at 25 DEG C after 20 weeks
Obtain the little young ball of Bulbus Lilii, little young ball is selected and carried out intermittent warming, finds after process: (1) outer bulb, middle bulb cuttage growth
The little young ball gone out all exists 100～900mg, and interior bulb fails to grow the young ball of 700～900mg, it may be possible to due to interior squama
The nutrient accumulation of stem is insufficient, fails to provide sufficient nutrition to little young ball.The ability of scale formation 600～900mg son's ball is by height
To low it is: outer scale ＞ middle level scale ＞ internal layer scale；The ability of scale formation 300～600mg son's ball by height is on earth: in
Layer scale ＞ outer scale ＞ internal layer scale；Scale forms 100～300mg.(2) in the test relenting time-triggered protocol, warp
Bulbus Lilii son's ball of the intermittent warming crossing 15 DEG C is more vulnerable to certain induction than Bulbus Lilii son's ball of 11 DEG C, 19 DEG C intermittent warming, promotes
Trunk diameter growth rate, produces phase transformation.(3) room temperature process induced transformation research in, the lily bulb of cuttage through 20 weeks 25 DEG C
Room temperature process, 4 weeks 15 DEG C of intermittent warming and the generation of 10 weeks K cryogenic treatment induced transformations preferably of 4 DEG C.(4) each level squama
On sheet, different young ball sizes are in the impact of young ball phase transformation, find that the young ball that young ball specific mass that quality is big is little is more easily generated phase
Become.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, and any
Those familiar with the art, in the technical scope that the invention discloses, can readily occur in change or replace, should contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should described be as the criterion with scope of the claims.
Claims (6)
1. the method for intermittent warming Bulbus Lilii son's ball phase transformation, it is characterised in that: take healthy lily ball, with bulb for according to dividing
For outer, in, interior different levels carry out classification cuttage；General planting is carried out after being divided into three to process；Room temperature 25 DEG C processes 20 weeks, after
Continuous intermittent warming, breaking dormancy.
2. the method for intermittent warming Bulbus Lilii son's ball phase transformation as claimed in claim 2, it is characterised in that: at the peat composed of rotten mosses: Vermiculitum: Margarita
Rock is in the substrate of 2:1:1, and cuttage outer layer, middle level, internal layer scale, after 25 DEG C of cuttages 20 weeks, random to three layers of scale respectively
Choosing 300 pieces, add up the young ball weight formed on each layer scale, grade scale is as follows: 800～900mg, 700～800mg, 600
～700mg, 500～600mg, 400～500mg, 300～400mg, 200～300mg, 100～200mg.Add up each heavyweight
The quantity of young ball is generated on each layer scale.
3. the method for intermittent warming Bulbus Lilii son's ball phase transformation as claimed in claim 2, it is characterised in that: select under the conditions of 25 DEG C
The outer scale of Bulbus Lilii " Robina " kind after cuttage 20 weeks, selects the little young ball of weight Bulbus Lilii in the range of 500-600mg,
Little young ball is carried out the process of temperature and time, then breaking dormancy K cryogenic treatment 10 under the conditions of 4 DEG C during intermittent warming
Week.
4. the method for intermittent warming Bulbus Lilii son's ball phase transformation as claimed in claim 2, it is characterised in that: Bulbus Lilii outer scale is entered
Row cuttage, extends cutting time, adjusts room temperature and processes the time, selects weight at the young ball of 500-600mg scope, carries out young ball
4 weeks intermittent warming of 15 DEG C, then it is carried out 4 DEG C of K cryogenic treatment 10 weeks, breaking dormancy.
5. the method for intermittent warming Bulbus Lilii son's ball phase transformation as claimed in claim 2, it is characterised in that: select Bulbus Lilii high-quality kind ball
To outer layer, middle level, the classification cuttage of internal layer scale, cuttage 20 weeks under the conditions of 25 DEG C, the young ball forming scale cuttage carries out weight
Amount classification, grade scale is as follows: 800～900mg, 700～800mg, 600～700mg, 500～600mg, 400～500mg,
300～400mg, 200～300mg, 100～200mg, 8 grades；Then all young balls are preserved 4 weeks under the conditions of 15 DEG C,
K cryogenic treatment 10 weeks under the conditions of 4 DEG C.After in basket general planting cultivate.
6. the method for intermittent warming Bulbus Lilii son's ball phase transformation as claimed in claim 2, it is characterised in that: described lily ball is strong
Health OT Bulbus Lilii Robina kind ball.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109463277A (en) * | 2018-11-23 | 2019-03-15 | 北京农学院 | Hybrid lily offspring's high-efficiency in-vitro balling-up and the method for shortening juvenile phase |
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| CN101711493A (en) * | 2009-11-11 | 2010-05-26 | 玉溪明珠花卉股份有限公司 | Propagation of lily seedball by directly peeling scale |
| CN102484967A (en) * | 2010-12-02 | 2012-06-06 | 辽宁省农业科学院花卉研究所 | Breeding method for oriental lily seedball |
| CN102577804A (en) * | 2012-03-01 | 2012-07-18 | 玉溪明珠花卉股份有限公司 | Method for increasing rate of scale cuttage propagation of lily |
-
2016
- 2016-07-19 CN CN201610570494.6A patent/CN106171890A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101711493A (en) * | 2009-11-11 | 2010-05-26 | 玉溪明珠花卉股份有限公司 | Propagation of lily seedball by directly peeling scale |
| CN102484967A (en) * | 2010-12-02 | 2012-06-06 | 辽宁省农业科学院花卉研究所 | Breeding method for oriental lily seedball |
| CN102577804A (en) * | 2012-03-01 | 2012-07-18 | 玉溪明珠花卉股份有限公司 | Method for increasing rate of scale cuttage propagation of lily |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109463277A (en) * | 2018-11-23 | 2019-03-15 | 北京农学院 | Hybrid lily offspring's high-efficiency in-vitro balling-up and the method for shortening juvenile phase |
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Application publication date: 20161207 |