CN106171347A - A kind of lily bud scale cottage method - Google Patents

Abstract

One lily bud scale cottage method of the present invention, a kind of method disclosing reproductive efficiency improving the mitogenetic clove of lily bud scale, it is applied to lily bud scale propagation production system.This invention relate to a kind of reagent A and processing method, it is possible to makes scale mitogenetic bulbec within a short period of time of process under normal temperature environment, and has higher differentiation rate.Invention significantly improves lily bud scale cuttage efficiency, to improving lily bud scale Reproduction efficiency, there is important actual application value.The present invention is A reagent for improving the reagent of scale lining rate, can not only significantly shorten through this agent treated and germinate the small seed ball time, and breeding coefficient increases.

Description

A kind of lily bud scale cottage method

Technical field

The present invention relates to method for propagation technical field, particularly relate to a kind of Bulbus Lilii cottage method.

Background technology

Bulbus Lilii propagation method has two kinds: sexual propagation (seminal propagation) and asexual propagation.Seminal propagation growth is slow, typically needs Taking 3 years can bloom and the most solid, the most only new PE curriculum uses seminal propagation to produce, and other all kinds Bulbus Liliies are equal For clone, vegetative manner expanding propagation can only be passed through.Asexual reproduction method mainly has following several: division propagation, pearl Bud breeding, tissue culture propagation and scale lining (Dan Yan etc., 2006).Division propagation can only obtain 1-3 clove in 1 year, such as volume Pellet, reproduction speed is slow, and breeding coefficient is low, the most easily causes the degeneration of lily cultivar；Bulbil breeding can be only applied to spy Fixed kind, such as tiger lily, Lilium sulphureum Baker and phonetic loan characters three kinds；Though tissue culture propagation improves breeding coefficient, but required skill Art and production cost are high, are difficult to by general Producer popularization and application；Scale cuttage technique is most-often used in Bulbus Lilii breeding Method, the method low cost, simple to operate and breeding coefficient is high (Liu Renkun etc., 2010).The scale of Bulbus Lilii is a lot, each squama Sheet base portion can produce several clove, and each clove can develop into again a complete plant, such a kind of ball warp squama Typically can produce up to a hundred after sheet cuttage and plant ball, the method simplicity is easy to spread, is now widely used for the rule of lily ball Modelling and in commercially producing (Van Tuyl, 1996).

Lily bud scale is the organ of multiplication breeding new life, for some Bulbus Lilii original seeds, though tissue culture technique can reach To detoxification, the most numerous purpose, but investment of production is big, required technical merit, relatively costly, seldom straight in large-scale production Connect use group and cultivate ball.Scale cuttage is widely used in the production of Bulbus Lilii, general each kind of ball peelable scale 10-20 sheet, Each scale can obtain 1 by cuttage and just can form commodity kind ball to multiple small seed balls, small seed ball plantation 2-3, be applied to cut-flower Produce and in garden cultivation (Liu little Feng etc., 2009).This propagation method can reduce kind of ball cost more than 10 times, is scale kind ball Key technique in propagation production.

Scale cottage propagation substantially increases the breeding coefficient of Bulbus Lilii, thus is widely used in the large-scale production of Bulbus Lilii In.The factor affecting lily bud scale cottage propagation is a lot, and endogenous cause of ill has breediness and scale position, and exopathogenic factor includes temperature, humidity And various physically or chemically derivant processes.The scale cuttage technique of oriental hybrid lily ' rope freshwater mussel ' is carried out by Liu Renkun etc. (2010) Research, is tested, it is believed that at 9-10 from the disinfecting of early stage scale, scale level, cutting time and HORMONE TREATMENT Use outer scale cuttage gained breeding coefficient and scale utilization rate higher the moon, reach 3.72；Liu little Feng etc. (2009) are to 6 kinds of cultivations In the scale cuttage technique research of training Bulbus Lilii, draw in different cutting mediums and variable concentrations growth regulator, Yelloween All there are relatively low rotting rate and higher breeding coefficient compared with other kinds, reach 2.14；Niu Lixin etc. (2005) respectively from pretreatment, The cuttage technique of 4 kinds of oriental hybrid lilies has been done system and has ground by the several respects such as the screening of cutting medium, accelerating germination link, nutritional solution apolegamy Study carefully；Hao Ruijie (2012) etc. have studied hormon concentration and process time, the different cuttage degree of depth to lanzhou lily scale cuttage The impact of indices；External also have corresponding research, Matsuo and Van Tuyl (1986) points out scale holding conditions Intermittent warming, it is possible to fast breeding goes out clove, when initial temperature is 35 DEG C, some cuttage material i.e. produced little at about 20 days Bulb, but for the bulb of most of Lilium Pollyanna hybrids system, initial temperature can promote the formation of clove at 23 DEG C, it After temperature is reduced to 17 DEG C, the generation of stem can be induced.But above-mentioned these cottage method processes are the most loaded down with trivial details, often exist Early stage needs in processing just to complete (2010) such as cuttage experiment, Liu Renkuns more strict to temperature requirement through several processes Though having obtained higher breeding coefficient, but by seasonal effect too greatly, and the use of growth regulator also considerably increases cuttage The cost of breeding, these factors limit cottage propagation spread in production application.Traditional lily bud scale cuttage Method step is the most loaded down with trivial details, from initial scale Cold pretreatment, sterilization method, the use of growth regulator, cutting medium And the aspect such as cuttage season all explored, the most time-consuming, and add production cost, constrain its big on producing Area is promoted；And study and point out, it is not that cottage propagation coefficient is the highest more good, usual cuttage gained clove diameter and breeding Coefficient is inversely proportional to, i.e. breeding coefficient is the highest, and gained clove diameter is the least, and utilization rate aborning is the lowest, thus cuttage Breeding coefficient is that 2-3 is advisable.Therefore, the technical process of simplification lily bud scale cottage propagation, and improve the coefficient of cottage propagation, right In the scale of lily ball and commercially produce significant.

Summary of the invention

The present invention is directed to the many drawbacks in existing lily bud scale cottage propagation, it is provided that a kind of quickly raising cuttage is numerous Grow the reagent A of efficiency, and combine suitable disinfection way, the problem such as solve that rotting rate is high and breeding coefficient is low.

The method quickly improving lily bud scale cottage propagation efficiency, it is characterised in that the scale sterilized is soaked in and contains Have in certain density reagent A.Wherein the volume fraction of reagent A is 4%, and soak time is 12h.

The inventive method have employed the sterilized scale 12h of reagent A immersion treatment of 4%, and lower about 25 days of cuttage is the most raw Going out small seed ball, scale rotting rate is low, and obtain higher cottage propagation coefficient 2.25 (average each scale breed 2-3 little Seed ball), this cottage method is not affected by temperature and cuttage season, is suitable for large-scale production, introduces a fine variety popularization for Bulbus Lilii further Efficient technical scheme is provided.

Accompanying drawing explanation

Fig. 1 is that 25 days post processing groups of ' rope freshwater mussel ' cuttage grow comparison diagram with matched group.

Process group: the reagent A immersion treatment 12h scale through 4% go out ball situation.Matched group: unused reagent A immersion treatment Scale growing state after cuttage 25 days.

Fig. 2 is ' rope freshwater mussel ' scale growing state figure after reagent A processes 50 days.

After cuttage 50 days, process group with compare growing state figure.Process group: the reagent A of 4% processes scale growth under 12h Situation map；Matched group: the scale growing state figure processed without reagent A.

Fig. 3 is 60 days post processing groups of ' Siberia ' cuttage and compare growing state comparison diagram.

' Siberia ' process group with to ball rate comparison diagram of breaking forth.Process group: the reagent A process 12h through 4% goes out ball Figure；Comparison figure: the scale processed without reagent A goes out ball figure.

Detailed description of the invention

For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, specifically real below in conjunction with the present invention Execute example the technical scheme in the embodiment of the present invention is clearly and completely described.

1, choosing kind of a ball: choose growth consistent, ' rope freshwater mussel ' the Bulbus Lilii ball preserving about 50 days in freezer is test material, Put about next day of room temperature.

2, scale sterilization: stripping outer layer without the healthy and strong scale of pest and disease damage is test material, uses the Bravo of 800 times of liquid (thiophanate methyl or other disinfectant) is to scale soaking disinfection 20min.

3, present embodiment is A reagent for improving the reagent of lily bud scale cottage propagation coefficient.

4, present embodiment is provided with different Concentraton gradient and place to the reagent A improving lily bud scale cottage propagation coefficient The reason time, Concentraton gradient: 2%, 4%, 6%；Process time: 6h/12h/24h, design two factor three levels orthogonal test (see Table 1), totally 10 process combination, 50 scales of each combined treatment, and after scale immersion, cuttage is in the peat composed of rotten mosses, observes note at any time The growing state of record scale, counts ball scale number and goes out ball number (being shown in Table 2).

Table 1 factor level table

Table 1 The combination of the experiment

After scale is soaking, cuttage, in the peat composed of rotten mosses, is placed in outside greenhouse at random.Through the scale that reagent A immersion treatment is crossed, About 12 days scale base portions of cuttage i.e. start to expand, and start to bear small seed ball when 25 days；In matched group, cuttage started after 42 days Bear small seed ball.After lower 60 days differentiation rates of scale cuttage are stable, its long ball scale number, small seed ball sum and breeding coefficient are entered Row statistics, as shown in table 2.

From this table, after reagent A processes, process 5 and obtained the highest breeding coefficient 2.25, and under this process, Scale differentiation rate is up to 96%, is considered as the optimal processing combination of this test.

Table 2 orthogonal experiments analytical table

Table 2 Analyze of the experiment

Use T-inspection that the result of the test of optimal processing combination 5 and comparison is carried out variance analysis.

Group statistic

Independent sample is checked

Check through T-, it is known that the scale lining coefficient processed through reagent A is notable with contrast difference, thus through the reagent A of 4% Process 12h and can significantly improve the breeding coefficient of cuttage scale.

Gained breeding coefficient and forefathers research being contrasted, Liu little Feng etc. (2009) is to 6 kinds of oriental hybrid lily cultivars In the research of Cutting test, different soaking time, different cuttage environment, different growth regulator and different time lukewarm water are attempted Processing the impact on cottage propagation coefficient, though finally obtaining higher breeding coefficient 2.14, but institute is through complex steps, and cost is relatively High；Liu Renkun etc. (2010) disinfect from scale respectively, study squama in terms of scale level, cutting time and HORMONE TREATMENT 4 The optimum condition of sheet cottage propagation, breeding coefficient is up to 3.72, but this breeding coefficient is more serious by cuttage seasonal effect, must Must carry out in 9-10 month, and scale bear the small seed ball time needed for longer, be 35 days, more late than this experiment about 10 days, separately Although breeding coefficient is the highest in this experiment outer, but its small seed ball utilization rate is low, and seed ball maximum gauge is only 4 millimeters, thus I It can be seen that not breeding coefficient the highest more good, between 2-3, gained seed ball utilization rate is the highest；Yang Xun (2005) is to east In the research of side's lily bud scale Technique on Cuttage Propagation, use the nutritional solution seed soaking of different ratio to treat cuttage scale, and done accelerating germination Processing, breeding coefficient reaches 2.68, but initially germinating small seed ball required time is relatively long, needs about 5-8 week.Cottage propagation in the past Research in, carried out multi-step process in cuttage early stage, and added growth regulator and nutritional solution, in a disguised form added cuttage The cost of breeding, not from actually improving reproductive efficiency problem.This test is from improving reproductive efficiency and breeding coefficient for research Point of penetration, while improving breeding coefficient, shortens out again the ball time, and method is simple, is not required to the equipment of complexity With expensive reagent, it is easy to spread.

5, the optimal processing utilizing gained in 4 combines different lily cultivar (such as, tiger lily, ' Siberia ' etc. Deng), all obtain higher breeding coefficient.

6, the method is in October, 15, December and 16 years 3 monthly overtestings that carry out, and is not limited season by cuttage.

Claims (5)

1. a Bulbus Lilii cottage method, it is characterised in that including: lily bud scale is carried out immersion treatment, described reagent through reagent A A is polysorbas20；Described lily bud scale is utilized to carry out cuttage.

Method the most according to claim 1, it is characterised in that the volume fraction of described reagent A is 2%-6%.

Method the most according to claim 1, it is characterised in that the volume fraction of described reagent A is 4%.

4. according to the method described in claim 1-3 any one, it is characterised in that the time of described immersion treatment is that 6-24 is little Time.

5. according to the method described in claim 1-4 any one, it is characterised in that the time of described immersion treatment is 12 little Time.